Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1337420 | AES97386.1 | 72.222 | 108 | 29 | 1 | 69 | 175 | 2 | 109 | 2.77e-45 | 155 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1337420 | G7JX45 | 72.222 | 108 | 29 | 1 | 69 | 175 | 2 | 109 | 1.32e-45 | 155 |
| Gene ID | Type | Classification |
|---|---|---|
| Msa1337420 | TF | B3 |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 32 sgRNAs with CRISPR-Local
Find 126 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CAGACTTCAATGATTTCTTA+TGG | 0.226332 | tig0001526:+88972 | Msa1337420:CDS |
| AATATATACAAAACCTGATA+TGG | 0.287652 | tig0001526:-88370 | None:intergenic |
| AACTGTACATCTTCCATATC+AGG | 0.290482 | tig0001526:+88357 | Msa1337420:CDS |
| TGTACTCAAACGACAAGTTT+TGG | 0.321936 | tig0001526:+88324 | Msa1337420:CDS |
| GAGACATTCTGTAACAATAA+TGG | 0.322182 | tig0001526:+89112 | Msa1337420:CDS |
| GGAATTCTTCAAGAAACTTT+GGG | 0.343998 | tig0001526:-88525 | None:intergenic |
| TGGAATTCTTCAAGAAACTT+TGG | 0.344507 | tig0001526:-88526 | None:intergenic |
| TCCAAACTGGTTTCAAATCA+TGG | 0.348731 | tig0001526:-88546 | None:intergenic |
| AATGTAACTTTCAACAATTC+AGG | 0.360324 | tig0001526:+88598 | Msa1337420:CDS |
| TCCATGATTTGAAACCAGTT+TGG | 0.362429 | tig0001526:+88545 | Msa1337420:CDS |
| AGACTTCAATGATTTCTTAT+GGG | 0.387413 | tig0001526:+88973 | Msa1337420:CDS |
| TTATTATGTCCACAGGAATT+CGG | 0.416128 | tig0001526:+88493 | Msa1337420:intron |
| TTCTTTCTACTTCAAATGGC+TGG | 0.419263 | tig0001526:+88292 | None:intergenic |
| TATTCAGGGATTCATTGCTT+TGG | 0.427325 | tig0001526:-89069 | None:intergenic |
| TTTGTCTCATGCCTTCGAAA+TGG | 0.428599 | tig0001526:-88669 | None:intergenic |
| AACGGATAGTGTCTCCAGTC+TGG | 0.449616 | tig0001526:-89138 | None:intergenic |
| CATAAGAAATCATTGAAGTC+TGG | 0.456714 | tig0001526:-88971 | None:intergenic |
| TCCCATCGAAGAACTTGAAA+CGG | 0.464329 | tig0001526:-89156 | None:intergenic |
| GTCCGTTTGTTCATGTCTAC+AGG | 0.467630 | tig0001526:+89185 | Msa1337420:CDS |
| ATCCGTTTCAAGTTCTTCGA+TGG | 0.488393 | tig0001526:+89154 | Msa1337420:CDS |
| ACATCTATCAGATTCCAAAC+TGG | 0.492899 | tig0001526:-88559 | None:intergenic |
| ATCTATCCTTTACTGACTAC+AGG | 0.503065 | tig0001526:+88622 | Msa1337420:CDS |
| TCCGTTTCAAGTTCTTCGAT+GGG | 0.508162 | tig0001526:+89155 | Msa1337420:CDS |
| AACAATAATGGATTCCAGAC+TGG | 0.518151 | tig0001526:+89124 | Msa1337420:CDS |
| TGGAATCTGATAGATGTTGA+TGG | 0.518673 | tig0001526:+88565 | Msa1337420:CDS |
| AGAGATTCTTTCCATTTCGA+AGG | 0.525219 | tig0001526:+88658 | Msa1337420:CDS |
| GCTGTAACGAACTCATCATT+CGG | 0.528661 | tig0001526:-89034 | None:intergenic |
| TTCCTGTAGACATGAACAAA+CGG | 0.548025 | tig0001526:-89187 | None:intergenic |
| GTCCATCCTGTAGTCAGTAA+AGG | 0.571628 | tig0001526:-88628 | None:intergenic |
| GAAATGGAAAGAATCTCTGA+TGG | 0.583229 | tig0001526:-88653 | None:intergenic |
| ATCCTTTACTGACTACAGGA+TGG | 0.606783 | tig0001526:+88626 | Msa1337420:CDS |
| AAGAAATCATTGAAGTCTGG+TGG | 0.613745 | tig0001526:-88968 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ACATAATTTGGTTATAATAA+AGG | - | tig0001526:88899-88918 | None:intergenic | 15.0% |
| !! | ACATAATTTGGTTATAATAA+AGG | - | tig0001526:88899-88918 | None:intergenic | 15.0% |
| !! | AATATATACAAAACCTGATA+TGG | - | tig0001526:88373-88392 | None:intergenic | 20.0% |
| !!! | TTAAACAGAGTATTTAGTAA+AGG | - | tig0001526:88398-88417 | None:intergenic | 20.0% |
| !! | TGTCAAAAAAAAATGATTTG+CGG | - | tig0001526:88804-88823 | None:intergenic | 20.0% |
| !! | AAAACATGTATTTGAGAATT+TGG | - | tig0001526:88931-88950 | None:intergenic | 20.0% |
| !!! | GTTTTTTAACTTAGTATTCA+GGG | - | tig0001526:89086-89105 | None:intergenic | 20.0% |
| !! | CTGAATACTAAGTTAAAAAA+CGG | + | tig0001526:89085-89104 | Msa1337420:CDS | 20.0% |
| !! | AATATATACAAAACCTGATA+TGG | - | tig0001526:88373-88392 | None:intergenic | 20.0% |
| !!! | TTAAACAGAGTATTTAGTAA+AGG | - | tig0001526:88398-88417 | None:intergenic | 20.0% |
| !! | TGTCAAAAAAAAATGATTTG+CGG | - | tig0001526:88804-88823 | None:intergenic | 20.0% |
| !! | AAAACATGTATTTGAGAATT+TGG | - | tig0001526:88931-88950 | None:intergenic | 20.0% |
| !!! | GTTTTTTAACTTAGTATTCA+GGG | - | tig0001526:89086-89105 | None:intergenic | 20.0% |
| !! | CTGAATACTAAGTTAAAAAA+CGG | + | tig0001526:89085-89104 | Msa1337420:CDS | 20.0% |
| ! | AATGTAACTTTCAACAATTC+AGG | + | tig0001526:88598-88617 | Msa1337420:CDS | 25.0% |
| !! | AAAAAAAATGATTTGCGGTT+CGG | - | tig0001526:88799-88818 | None:intergenic | 25.0% |
| ! | TGGTGAATAAGTACATAATT+TGG | - | tig0001526:88911-88930 | None:intergenic | 25.0% |
| !! | ATACATGTTTTGCAAATTAC+AGG | + | tig0001526:88940-88959 | Msa1337420:intron | 25.0% |
| ! | AGACTTCAATGATTTCTTAT+GGG | + | tig0001526:88973-88992 | Msa1337420:CDS | 25.0% |
| !!! | CGTTTTTTAACTTAGTATTC+AGG | - | tig0001526:89087-89106 | None:intergenic | 25.0% |
| ! | ATACTAAGTTAAAAAACGGT+TGG | + | tig0001526:89089-89108 | Msa1337420:CDS | 25.0% |
| ! | TACTAAGTTAAAAAACGGTT+GGG | + | tig0001526:89090-89109 | Msa1337420:CDS | 25.0% |
| ! | AATGTAACTTTCAACAATTC+AGG | + | tig0001526:88598-88617 | Msa1337420:CDS | 25.0% |
| !! | AAAAAAAATGATTTGCGGTT+CGG | - | tig0001526:88799-88818 | None:intergenic | 25.0% |
| ! | TGGTGAATAAGTACATAATT+TGG | - | tig0001526:88911-88930 | None:intergenic | 25.0% |
| !! | ATACATGTTTTGCAAATTAC+AGG | + | tig0001526:88940-88959 | Msa1337420:intron | 25.0% |
| ! | AGACTTCAATGATTTCTTAT+GGG | + | tig0001526:88973-88992 | Msa1337420:CDS | 25.0% |
| !!! | CGTTTTTTAACTTAGTATTC+AGG | - | tig0001526:89087-89106 | None:intergenic | 25.0% |
| ! | ATACTAAGTTAAAAAACGGT+TGG | + | tig0001526:89089-89108 | Msa1337420:CDS | 25.0% |
| ! | TACTAAGTTAAAAAACGGTT+GGG | + | tig0001526:89090-89109 | Msa1337420:CDS | 25.0% |
| TCTGATTAAGTTAGACAACA+TGG | - | tig0001526:88438-88457 | None:intergenic | 30.0% | |
| CAAATGTTTATTATGTCCAC+AGG | + | tig0001526:88486-88505 | Msa1337420:intron | 30.0% | |
| TTATTATGTCCACAGGAATT+CGG | + | tig0001526:88493-88512 | Msa1337420:intron | 30.0% | |
| GGAATTCTTCAAGAAACTTT+GGG | - | tig0001526:88528-88547 | None:intergenic | 30.0% | |
| TGGAATTCTTCAAGAAACTT+TGG | - | tig0001526:88529-88548 | None:intergenic | 30.0% | |
| ! | CACTTTGACTAATTTGCATA+TGG | + | tig0001526:88825-88844 | Msa1337420:intron | 30.0% |
| CATAAGAAATCATTGAAGTC+TGG | - | tig0001526:88974-88993 | None:intergenic | 30.0% | |
| CAGACTTCAATGATTTCTTA+TGG | + | tig0001526:88972-88991 | Msa1337420:CDS | 30.0% | |
| ! | GATTTTCATTGCACAGAAAA+AGG | - | tig0001526:89015-89034 | None:intergenic | 30.0% |
| GAGACATTCTGTAACAATAA+TGG | + | tig0001526:89112-89131 | Msa1337420:CDS | 30.0% | |
| TCTGATTAAGTTAGACAACA+TGG | - | tig0001526:88438-88457 | None:intergenic | 30.0% | |
| CAAATGTTTATTATGTCCAC+AGG | + | tig0001526:88486-88505 | Msa1337420:intron | 30.0% | |
| TTATTATGTCCACAGGAATT+CGG | + | tig0001526:88493-88512 | Msa1337420:intron | 30.0% | |
| GGAATTCTTCAAGAAACTTT+GGG | - | tig0001526:88528-88547 | None:intergenic | 30.0% | |
| TGGAATTCTTCAAGAAACTT+TGG | - | tig0001526:88529-88548 | None:intergenic | 30.0% | |
| ! | CACTTTGACTAATTTGCATA+TGG | + | tig0001526:88825-88844 | Msa1337420:intron | 30.0% |
| CATAAGAAATCATTGAAGTC+TGG | - | tig0001526:88974-88993 | None:intergenic | 30.0% | |
| CAGACTTCAATGATTTCTTA+TGG | + | tig0001526:88972-88991 | Msa1337420:CDS | 30.0% | |
| ! | GATTTTCATTGCACAGAAAA+AGG | - | tig0001526:89015-89034 | None:intergenic | 30.0% |
| GAGACATTCTGTAACAATAA+TGG | + | tig0001526:89112-89131 | Msa1337420:CDS | 30.0% | |
| ! | TGTACTCAAACGACAAGTTT+TGG | + | tig0001526:88324-88343 | Msa1337420:CDS | 35.0% |
| ! | GTACTCAAACGACAAGTTTT+GGG | + | tig0001526:88325-88344 | Msa1337420:CDS | 35.0% |
| AACTGTACATCTTCCATATC+AGG | + | tig0001526:88357-88376 | Msa1337420:CDS | 35.0% | |
| ! | TCCATGATTTGAAACCAGTT+TGG | + | tig0001526:88545-88564 | Msa1337420:CDS | 35.0% |
| ! | TCCAAACTGGTTTCAAATCA+TGG | - | tig0001526:88549-88568 | None:intergenic | 35.0% |
| ACATCTATCAGATTCCAAAC+TGG | - | tig0001526:88562-88581 | None:intergenic | 35.0% | |
| TGGAATCTGATAGATGTTGA+TGG | + | tig0001526:88565-88584 | Msa1337420:CDS | 35.0% | |
| ! | ATCTATCCTTTACTGACTAC+AGG | + | tig0001526:88622-88641 | Msa1337420:CDS | 35.0% |
| GAAATGGAAAGAATCTCTGA+TGG | - | tig0001526:88656-88675 | None:intergenic | 35.0% | |
| AGAGATTCTTTCCATTTCGA+AGG | + | tig0001526:88658-88677 | Msa1337420:CDS | 35.0% | |
| TATGGCAGTTAGATCTGAAT+TGG | + | tig0001526:88843-88862 | Msa1337420:intron | 35.0% | |
| AAGAAATCATTGAAGTCTGG+TGG | - | tig0001526:88971-88990 | None:intergenic | 35.0% | |
| TATTCAGGGATTCATTGCTT+TGG | - | tig0001526:89072-89091 | None:intergenic | 35.0% | |
| !! | AACAATAATGGATTCCAGAC+TGG | + | tig0001526:89124-89143 | Msa1337420:CDS | 35.0% |
| TTCCTGTAGACATGAACAAA+CGG | - | tig0001526:89190-89209 | None:intergenic | 35.0% | |
| ! | TGTACTCAAACGACAAGTTT+TGG | + | tig0001526:88324-88343 | Msa1337420:CDS | 35.0% |
| ! | GTACTCAAACGACAAGTTTT+GGG | + | tig0001526:88325-88344 | Msa1337420:CDS | 35.0% |
| AACTGTACATCTTCCATATC+AGG | + | tig0001526:88357-88376 | Msa1337420:CDS | 35.0% | |
| ! | TCCATGATTTGAAACCAGTT+TGG | + | tig0001526:88545-88564 | Msa1337420:CDS | 35.0% |
| ! | TCCAAACTGGTTTCAAATCA+TGG | - | tig0001526:88549-88568 | None:intergenic | 35.0% |
| ACATCTATCAGATTCCAAAC+TGG | - | tig0001526:88562-88581 | None:intergenic | 35.0% | |
| TGGAATCTGATAGATGTTGA+TGG | + | tig0001526:88565-88584 | Msa1337420:CDS | 35.0% | |
| ! | ATCTATCCTTTACTGACTAC+AGG | + | tig0001526:88622-88641 | Msa1337420:CDS | 35.0% |
| GAAATGGAAAGAATCTCTGA+TGG | - | tig0001526:88656-88675 | None:intergenic | 35.0% | |
| AGAGATTCTTTCCATTTCGA+AGG | + | tig0001526:88658-88677 | Msa1337420:CDS | 35.0% | |
| TATGGCAGTTAGATCTGAAT+TGG | + | tig0001526:88843-88862 | Msa1337420:intron | 35.0% | |
| AAGAAATCATTGAAGTCTGG+TGG | - | tig0001526:88971-88990 | None:intergenic | 35.0% | |
| TATTCAGGGATTCATTGCTT+TGG | - | tig0001526:89072-89091 | None:intergenic | 35.0% | |
| !! | AACAATAATGGATTCCAGAC+TGG | + | tig0001526:89124-89143 | Msa1337420:CDS | 35.0% |
| TTCCTGTAGACATGAACAAA+CGG | - | tig0001526:89190-89209 | None:intergenic | 35.0% | |
| ! | ATCCTTTACTGACTACAGGA+TGG | + | tig0001526:88626-88645 | Msa1337420:CDS | 40.0% |
| TTTGTCTCATGCCTTCGAAA+TGG | - | tig0001526:88672-88691 | None:intergenic | 40.0% | |
| CAAATCGTGCTACACTATGT+TGG | + | tig0001526:88688-88707 | Msa1337420:intron | 40.0% | |
| GCTGTAACGAACTCATCATT+CGG | - | tig0001526:89037-89056 | None:intergenic | 40.0% | |
| !! | ATCCGTTTCAAGTTCTTCGA+TGG | + | tig0001526:89154-89173 | Msa1337420:CDS | 40.0% |
| !! | TCCGTTTCAAGTTCTTCGAT+GGG | + | tig0001526:89155-89174 | Msa1337420:CDS | 40.0% |
| TCCCATCGAAGAACTTGAAA+CGG | - | tig0001526:89159-89178 | None:intergenic | 40.0% | |
| ! | ATCCTTTACTGACTACAGGA+TGG | + | tig0001526:88626-88645 | Msa1337420:CDS | 40.0% |
| TTTGTCTCATGCCTTCGAAA+TGG | - | tig0001526:88672-88691 | None:intergenic | 40.0% | |
| CAAATCGTGCTACACTATGT+TGG | + | tig0001526:88688-88707 | Msa1337420:intron | 40.0% | |
| GCTGTAACGAACTCATCATT+CGG | - | tig0001526:89037-89056 | None:intergenic | 40.0% | |
| !! | ATCCGTTTCAAGTTCTTCGA+TGG | + | tig0001526:89154-89173 | Msa1337420:CDS | 40.0% |
| !! | TCCGTTTCAAGTTCTTCGAT+GGG | + | tig0001526:89155-89174 | Msa1337420:CDS | 40.0% |
| TCCCATCGAAGAACTTGAAA+CGG | - | tig0001526:89159-89178 | None:intergenic | 40.0% | |
| !!! | CTCAAACGACAAGTTTTGGG+AGG | + | tig0001526:88328-88347 | Msa1337420:CDS | 45.0% |
| GTCCATCCTGTAGTCAGTAA+AGG | - | tig0001526:88631-88650 | None:intergenic | 45.0% | |
| TTCCAAGAGCGAGGATGAAT+CGG | - | tig0001526:88720-88739 | None:intergenic | 45.0% | |
| TTTGTGCTTCTGCTGTGGAA+GGG | - | tig0001526:88772-88791 | None:intergenic | 45.0% | |
| GTCCGTTTGTTCATGTCTAC+AGG | + | tig0001526:89185-89204 | Msa1337420:CDS | 45.0% | |
| !!! | CTCAAACGACAAGTTTTGGG+AGG | + | tig0001526:88328-88347 | Msa1337420:CDS | 45.0% |
| GTCCATCCTGTAGTCAGTAA+AGG | - | tig0001526:88631-88650 | None:intergenic | 45.0% | |
| TTCCAAGAGCGAGGATGAAT+CGG | - | tig0001526:88720-88739 | None:intergenic | 45.0% | |
| TTTGTGCTTCTGCTGTGGAA+GGG | - | tig0001526:88772-88791 | None:intergenic | 45.0% | |
| GTCCGTTTGTTCATGTCTAC+AGG | + | tig0001526:89185-89204 | Msa1337420:CDS | 45.0% | |
| TTCACTTCGCCGAATTCCTG+TGG | - | tig0001526:88505-88524 | None:intergenic | 50.0% | |
| GATTCATCCTCGCTCTTGGA+AGG | + | tig0001526:88719-88738 | Msa1337420:intron | 50.0% | |
| GAAGGGAGGGAAATCATCAG+TGG | - | tig0001526:88755-88774 | None:intergenic | 50.0% | |
| CTTTGTGCTTCTGCTGTGGA+AGG | - | tig0001526:88773-88792 | None:intergenic | 50.0% | |
| !! | GTAGCTTTGTGCTTCTGCTG+TGG | - | tig0001526:88777-88796 | None:intergenic | 50.0% |
| AACGGATAGTGTCTCCAGTC+TGG | - | tig0001526:89141-89160 | None:intergenic | 50.0% | |
| TTCACTTCGCCGAATTCCTG+TGG | - | tig0001526:88505-88524 | None:intergenic | 50.0% | |
| GATTCATCCTCGCTCTTGGA+AGG | + | tig0001526:88719-88738 | Msa1337420:intron | 50.0% | |
| GAAGGGAGGGAAATCATCAG+TGG | - | tig0001526:88755-88774 | None:intergenic | 50.0% | |
| CTTTGTGCTTCTGCTGTGGA+AGG | - | tig0001526:88773-88792 | None:intergenic | 50.0% | |
| !! | GTAGCTTTGTGCTTCTGCTG+TGG | - | tig0001526:88777-88796 | None:intergenic | 50.0% |
| AACGGATAGTGTCTCCAGTC+TGG | - | tig0001526:89141-89160 | None:intergenic | 50.0% | |
| ! | GAGCGAGGATGAATCGGCTT+TGG | - | tig0001526:88714-88733 | None:intergenic | 55.0% |
| ! | AGCCGATTCATCCTCGCTCT+TGG | + | tig0001526:88715-88734 | Msa1337420:intron | 55.0% |
| ! | GAAGTGTCCTTCCAAGAGCG+AGG | - | tig0001526:88729-88748 | None:intergenic | 55.0% |
| TGCTTCTGCTGTGGAAGGGA+GGG | - | tig0001526:88768-88787 | None:intergenic | 55.0% | |
| ! | GAGCGAGGATGAATCGGCTT+TGG | - | tig0001526:88714-88733 | None:intergenic | 55.0% |
| ! | AGCCGATTCATCCTCGCTCT+TGG | + | tig0001526:88715-88734 | Msa1337420:intron | 55.0% |
| ! | GAAGTGTCCTTCCAAGAGCG+AGG | - | tig0001526:88729-88748 | None:intergenic | 55.0% |
| TGCTTCTGCTGTGGAAGGGA+GGG | - | tig0001526:88768-88787 | None:intergenic | 55.0% | |
| GTGCTTCTGCTGTGGAAGGG+AGG | - | tig0001526:88769-88788 | None:intergenic | 60.0% | |
| GTGCTTCTGCTGTGGAAGGG+AGG | - | tig0001526:88769-88788 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| tig0001526 | gene | 88307 | 89219 | 88307 | ID=Msa1337420;Name=Msa1337420 |
| tig0001526 | mRNA | 88307 | 89219 | 88307 | ID=Msa1337420-mRNA-1;Parent=Msa1337420;Name=Msa1337420-mRNA-1;_AED=0.45;_eAED=0.45;_QI=0|0|0|1|0.5|0.66|3|0|175 |
| tig0001526 | exon | 88307 | 88378 | 88307 | ID=Msa1337420-mRNA-1:exon:1166;Parent=Msa1337420-mRNA-1 |
| tig0001526 | exon | 88508 | 88705 | 88508 | ID=Msa1337420-mRNA-1:exon:1167;Parent=Msa1337420-mRNA-1 |
| tig0001526 | exon | 88962 | 89219 | 88962 | ID=Msa1337420-mRNA-1:exon:1168;Parent=Msa1337420-mRNA-1 |
| tig0001526 | CDS | 88307 | 88378 | 88307 | ID=Msa1337420-mRNA-1:cds;Parent=Msa1337420-mRNA-1 |
| tig0001526 | CDS | 88508 | 88705 | 88508 | ID=Msa1337420-mRNA-1:cds;Parent=Msa1337420-mRNA-1 |
| tig0001526 | CDS | 88962 | 89219 | 88962 | ID=Msa1337420-mRNA-1:cds;Parent=Msa1337420-mRNA-1 |
| Gene Sequence |
| Protein sequence |
