AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1339000

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1339000 MtrunA17_Chr7g0264171 94.382 178 10 0 1 178 87 264 2.76e-122 357
Msa1339000 MtrunA17_Chr7g0264161 88.750 160 18 0 1 160 48 207 6.75e-104 300
Msa1339000 MtrunA17_Chr2g0326761 61.364 176 63 2 1 176 67 237 1.62e-66 215
Msa1339000 MtrunA17_Chr1g0199681 66.216 148 50 0 1 148 149 296 2.96e-64 209
Msa1339000 MtrunA17_Chr1g0181211 65.269 167 48 4 1 160 83 246 3.76e-63 206
Msa1339000 MtrunA17_Chr4g0028971 59.444 180 69 3 1 177 66 244 3.75e-61 201
Msa1339000 MtrunA17_Chr3g0115271 54.667 150 61 2 1 148 76 220 6.45e-49 166
Msa1339000 MtrunA17_Chr8g0389081 52.000 150 66 2 2 149 95 240 2.92e-46 161
Msa1339000 MtrunA17_Chr1g0156321 50.331 151 72 1 2 152 106 253 5.73e-46 160
Msa1339000 MtrunA17_Chr4g0070181 48.667 150 71 2 2 149 85 230 3.07e-44 155
Msa1339000 MtrunA17_Chr2g0301251 49.333 150 70 2 2 149 85 230 7.70e-44 154
Msa1339000 MtrunA17_Chr2g0285461 48.026 152 75 2 2 152 86 234 4.50e-42 149
Msa1339000 MtrunA17_Chr2g0282961 48.684 152 71 1 2 146 108 259 1.08e-41 147
Msa1339000 MtrunA17_Chr5g0424931 50.000 150 61 2 1 145 78 218 1.16e-41 146
Msa1339000 MtrunA17_Chr2g0276781 47.682 151 74 2 2 151 85 231 4.94e-41 146
Msa1339000 MtrunA17_Chr4g0029671 48.428 159 74 1 1 151 114 272 8.57e-41 145
Msa1339000 MtrunA17_Chr3g0109391 48.366 153 73 3 2 152 89 237 1.77e-40 145
Msa1339000 MtrunA17_Chr5g0433301 47.712 153 74 3 2 152 87 235 2.38e-40 144
Msa1339000 MtrunA17_Chr2g0289221 47.682 151 73 3 1 148 80 227 5.73e-40 139
Msa1339000 MtrunA17_Chr8g0376771 46.980 149 73 2 2 148 85 229 6.98e-40 143
Msa1339000 MtrunA17_Chr4g0046371 46.203 158 74 1 2 148 94 251 8.99e-40 142
Msa1339000 MtrunA17_Chr2g0326281 45.349 172 73 3 1 151 116 287 1.05e-39 142
Msa1339000 MtrunA17_Chr5g0417281 46.753 154 69 3 1 148 70 216 1.09e-39 140
Msa1339000 MtrunA17_Chr4g0009751 47.333 150 74 2 1 149 80 225 7.49e-39 139
Msa1339000 MtrunA17_Chr8g0385791 47.682 151 73 3 2 150 121 267 1.41e-38 139
Msa1339000 MtrunA17_Chr8g0346711 46.711 152 76 2 1 151 90 237 1.49e-37 136
Msa1339000 MtrunA17_Chr7g0239061 46.528 144 74 2 1 144 75 215 4.39e-37 135
Msa1339000 MtrunA17_Chr5g0417211 45.513 156 69 3 1 147 71 219 3.37e-35 127
Msa1339000 MtrunA17_Chr5g0436761 43.312 157 72 3 1 147 71 220 1.65e-32 120
Msa1339000 MtrunA17_Chr1g0178011 41.892 148 78 2 1 145 71 213 3.18e-32 120
Msa1339000 MtrunA17_Chr2g0277461 41.781 146 82 3 1 145 74 217 1.88e-31 117
Msa1339000 MtrunA17_Chr5g0425661 38.028 142 70 3 1 133 93 225 6.29e-26 99.4
Msa1339000 MtrunA17_Chr8g0356211 42.424 99 57 0 53 151 2 100 3.93e-25 98.6
Msa1339000 MtrunA17_Chr2g0277531 37.838 148 85 5 1 145 81 224 3.15e-22 91.3
Msa1339000 MtrunA17_Chr5g0424801 32.667 150 76 5 6 148 76 207 1.48e-17 79.0
Msa1339000 MtrunA17_Chr5g0424741 31.977 172 88 6 1 145 71 240 8.18e-16 74.3
Msa1339000 MtrunA17_Chr5g0425261 32.000 150 77 5 6 148 76 207 1.82e-15 73.2
Msa1339000 MtrunA17_Chr8g0345771 31.496 127 82 2 40 162 167 292 2.16e-11 61.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1339000 AT2G28350.2 63.636 154 50 1 1 148 65 218 9.89e-63 205
Msa1339000 AT2G28350.1 63.636 154 50 1 1 148 65 218 1.44e-62 205
Msa1339000 AT4G30080.1 64.000 150 52 2 1 148 74 223 1.83e-59 196
Msa1339000 AT1G77850.2 50.000 148 72 1 1 146 73 220 6.74e-46 159
Msa1339000 AT1G77850.1 50.000 148 72 1 1 146 73 220 6.94e-46 159
Msa1339000 AT1G19220.1 50.667 150 68 2 2 149 85 230 1.46e-44 157
Msa1339000 AT5G20730.3 49.007 151 71 2 2 150 86 232 1.23e-43 154
Msa1339000 AT5G20730.2 49.007 151 71 2 2 150 86 232 1.32e-43 154
Msa1339000 AT5G20730.1 49.007 151 71 2 2 150 86 232 1.35e-43 154
Msa1339000 AT1G19850.1 49.007 151 75 1 2 152 117 265 6.25e-43 152
Msa1339000 AT1G19850.2 49.007 151 75 1 2 152 106 254 6.69e-43 152
Msa1339000 AT1G30330.1 49.669 151 74 2 2 152 86 234 2.91e-41 147
Msa1339000 AT1G30330.3 49.669 151 74 2 2 152 88 236 2.91e-41 147
Msa1339000 AT1G30330.2 49.669 151 74 2 2 152 88 236 2.91e-41 147
Msa1339000 AT5G60450.2 47.170 159 76 2 1 151 101 259 4.03e-41 147
Msa1339000 AT5G60450.1 47.170 159 76 2 1 151 125 283 4.32e-41 147
Msa1339000 AT5G37020.2 47.712 153 74 3 2 152 85 233 4.55e-41 147
Msa1339000 AT5G37020.1 47.712 153 74 3 2 152 85 233 6.18e-41 146
Msa1339000 AT1G59750.4 50.000 150 72 3 2 151 84 230 1.71e-40 144
Msa1339000 AT1G59750.2 50.000 150 72 3 2 151 84 230 1.78e-40 144
Msa1339000 AT1G59750.1 50.000 150 72 3 2 151 84 230 2.12e-40 144
Msa1339000 AT1G59750.3 50.000 150 72 3 2 151 84 230 2.12e-40 144
Msa1339000 AT2G33860.1 45.455 154 77 1 2 148 109 262 9.53e-40 142
Msa1339000 AT3G61830.2 44.805 154 76 2 2 152 37 184 1.82e-38 138
Msa1339000 AT3G61830.1 44.805 154 76 2 2 152 88 235 3.24e-38 138
Msa1339000 AT5G62000.2 45.695 151 76 2 2 150 123 269 1.13e-37 137
Msa1339000 AT5G62000.5 45.695 151 76 2 2 150 123 269 1.13e-37 137
Msa1339000 AT5G62000.3 45.695 151 76 2 2 150 123 269 1.13e-37 137
Msa1339000 AT5G62000.1 45.695 151 76 2 2 150 123 269 1.13e-37 137
Msa1339000 AT5G62000.4 45.695 151 76 2 2 150 123 269 1.22e-37 137
Msa1339000 AT5G37020.3 54.630 108 49 0 45 152 31 138 1.96e-37 136
Msa1339000 AT4G23980.2 46.575 146 74 2 2 146 75 217 3.20e-37 135
Msa1339000 AT4G23980.1 46.575 146 74 2 2 146 75 217 3.57e-37 135
Msa1339000 AT2G33860.2 56.731 104 45 0 45 148 2 105 5.04e-37 133
Msa1339000 AT2G46530.1 41.420 169 89 3 2 166 84 246 1.09e-36 134
Msa1339000 AT2G46530.3 41.420 169 89 3 2 166 105 267 1.26e-36 134
Msa1339000 AT1G35520.1 44.295 149 77 2 2 150 89 231 4.55e-34 126
Msa1339000 AT1G35540.1 46.099 141 70 2 6 146 93 227 7.95e-34 125
Msa1339000 AT1G34310.1 43.448 145 76 1 6 150 93 231 3.57e-33 124
Msa1339000 AT1G35240.1 42.953 149 79 2 2 150 89 231 6.92e-33 123
Msa1339000 AT1G34410.1 43.709 151 75 2 2 150 89 231 1.20e-32 122
Msa1339000 AT2G46530.2 45.161 124 67 1 44 166 36 159 2.72e-32 121
Msa1339000 AT1G34390.1 44.138 145 73 2 2 146 89 225 6.47e-32 120
Msa1339000 AT1G34170.2 40.000 155 77 4 2 150 87 231 2.58e-28 109
Msa1339000 AT1G34170.1 40.000 155 77 4 2 150 87 231 3.05e-28 109
Msa1339000 AT1G34170.3 40.000 155 77 4 2 150 87 231 4.20e-28 109
Msa1339000 AT1G43950.1 39.568 139 75 4 6 144 93 222 3.31e-23 92.0
Msa1339000 AT2G46870.1 36.082 97 61 1 37 133 27 122 3.85e-13 66.6
Msa1339000 AT1G01030.2 34.545 110 66 2 40 144 51 159 3.16e-11 61.2
Msa1339000 AT3G25730.1 30.952 126 70 2 45 168 183 293 4.58e-11 60.8
Msa1339000 AT1G01030.1 34.545 110 66 2 40 144 51 159 4.83e-11 60.8
Msa1339000 AT4G01500.1 31.200 125 73 4 18 133 1 121 5.77e-11 60.5

Find 46 sgRNAs with CRISPR-Local

Find 53 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CATCAACGTGTCTGAAATTA+AGG 0.241218 tig0002988:+7487 Msa1339000:CDS
CCTTTGTCAGGGTGGAAATT+TGG 0.252828 tig0002988:+7857 Msa1339000:CDS
GCATTTCAGGCATGTTTATA+GGG 0.274751 tig0002988:+7676 Msa1339000:CDS
GGCATTTCAGGCATGTTTAT+AGG 0.293252 tig0002988:+7675 Msa1339000:CDS
ATGGTGAAAAGTGGCATTTC+AGG 0.316984 tig0002988:+7663 Msa1339000:CDS
TGGAAATTTGGTAGTGGAAT+TGG 0.321491 tig0002988:+7869 Msa1339000:CDS
TTGGAATTCGGAGGGCTAAA+AGG 0.363443 tig0002988:+7813 Msa1339000:CDS
TTTGATAATGATGGTGTTGC+TGG 0.376636 tig0002988:+7464 Msa1339000:CDS
GTTGGAGTCGATCCTTTGTC+AGG 0.383121 tig0002988:+7845 Msa1339000:CDS
AGTGACAAGAAACTTGCTTC+TGG 0.385156 tig0002988:+7746 Msa1339000:CDS
CTAACTTGGTTAATATTCAT+AGG 0.385591 tig0002988:-7440 Msa1339000:intergenic
AAGAGGCATTTGTTGACAAC+TGG 0.392584 tig0002988:+7707 Msa1339000:CDS
AGTTTCTTGTCACTGACATA+TGG 0.394738 tig0002988:-7737 Msa1339000:intergenic
GTGAACTTAATGTTGGAATT+CGG 0.402297 tig0002988:+7801 Msa1339000:CDS
GTGACAAGAAACTTGCTTCT+GGG 0.423889 tig0002988:+7747 Msa1339000:CDS
CATCATTATCAAAACTAACT+TGG 0.449861 tig0002988:-7454 Msa1339000:intergenic
GTTGTCAACAAATGCCTCTT+CGG 0.456311 tig0002988:-7704 Msa1339000:intergenic
ATTTGTCCTGCACCACCTTA+CGG 0.476461 tig0002988:+7893 Msa1339000:CDS
GAAAACAGTGAACTTAATGT+TGG 0.494744 tig0002988:+7794 Msa1339000:CDS
TCAGGGTGGAAATTTGGTAG+TGG 0.505938 tig0002988:+7863 Msa1339000:CDS
TTGGGATAGATGTCCTGAAA+AGG 0.529804 tig0002988:-7632 Msa1339000:intergenic
AATCCTCCGTAAGGTGGTGC+AGG 0.533772 tig0002988:-7899 Msa1339000:intergenic
TGACAAGAAACTTGCTTCTG+GGG 0.545499 tig0002988:+7748 Msa1339000:CDS
CCAAATTTCCACCCTGACAA+AGG 0.548832 tig0002988:-7857 Msa1339000:intergenic
GCTAAAAGGCGAAATAATGT+TGG 0.555377 tig0002988:+7827 Msa1339000:CDS
CATTTCAGGCATGTTTATAG+GGG 0.556177 tig0002988:+7677 Msa1339000:CDS
AACTATGGAAATCCTCCGTA+AGG 0.559080 tig0002988:-7908 Msa1339000:intergenic
AGCGTTTCGGCACAATATCT+AGG 0.559188 tig0002988:-7578 Msa1339000:intergenic
GGCATTTGTTGACAACTGGA+TGG 0.569244 tig0002988:+7711 Msa1339000:CDS
AACTTAATGTTGGAATTCGG+AGG 0.572730 tig0002988:+7804 Msa1339000:CDS
AGGATGTACATGGTGAAAAG+TGG 0.579569 tig0002988:+7654 Msa1339000:CDS
TCTGATGCAAACACTGGTGG+TGG 0.592766 tig0002988:+7545 Msa1339000:CDS
ATCTATCCCAAGGATGTACA+TGG 0.592936 tig0002988:+7644 Msa1339000:CDS
GGATAGATGTCCTGAAAAGG+AGG 0.594077 tig0002988:-7629 Msa1339000:intergenic
TTGGAGTCGATCCTTTGTCA+GGG 0.596740 tig0002988:+7846 Msa1339000:CDS
ATGAAGTGTATGCAAAACTC+AGG 0.607388 tig0002988:+7411 Msa1339000:CDS
TTTATAGGGGCACGCCGAAG+AGG 0.616360 tig0002988:+7690 Msa1339000:CDS
GTTTGCATCAGATTGTGTCA+AGG 0.616878 tig0002988:-7534 Msa1339000:intergenic
ACACAATCTGATGCAAACAC+TGG 0.627018 tig0002988:+7539 Msa1339000:CDS
ACTTAATGTTGGAATTCGGA+GGG 0.629032 tig0002988:+7805 Msa1339000:CDS
TATGGAAATCCTCCGTAAGG+TGG 0.634599 tig0002988:-7905 Msa1339000:intergenic
GAGTCGATCCTTTGTCAGGG+TGG 0.667216 tig0002988:+7849 Msa1339000:CDS
TGTCCTGCACCACCTTACGG+AGG 0.685773 tig0002988:+7896 Msa1339000:CDS
GATAGATGTCCTGAAAAGGA+GGG 0.691897 tig0002988:-7628 Msa1339000:intergenic
CAATCTGATGCAAACACTGG+TGG 0.725224 tig0002988:+7542 Msa1339000:CDS
TTAGCTGAATAGTCCAAACG+AGG 0.756003 tig0002988:-7605 Msa1338990:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! CTAACTTGGTTAATATTCAT+AGG - tig0002988:7443-7462 Msa1339000:intergenic 25.0%
! CATCATTATCAAAACTAACT+TGG - tig0002988:7457-7476 Msa1339000:intergenic 25.0%
!!! CAAGTTAGTTTTGATAATGA+TGG + tig0002988:7455-7474 Msa1339000:CDS 25.0%
GAAAACAGTGAACTTAATGT+TGG + tig0002988:7794-7813 Msa1339000:CDS 30.0%
GTGAACTTAATGTTGGAATT+CGG + tig0002988:7801-7820 Msa1339000:CDS 30.0%
! GAGTTTTGCATACACTTCAT+CGG - tig0002988:7411-7430 None:intergenic 35.0%
ATGAAGTGTATGCAAAACTC+AGG + tig0002988:7411-7430 Msa1339000:CDS 35.0%
!! TTTGATAATGATGGTGTTGC+TGG + tig0002988:7464-7483 Msa1339000:CDS 35.0%
! CATCAACGTGTCTGAAATTA+AGG + tig0002988:7487-7506 Msa1339000:CDS 35.0%
! TTTTCACCATGTACATCCTT+GGG - tig0002988:7653-7672 Msa1339000:intergenic 35.0%
GCATTTCAGGCATGTTTATA+GGG + tig0002988:7676-7695 Msa1339000:CDS 35.0%
CATTTCAGGCATGTTTATAG+GGG + tig0002988:7677-7696 Msa1339000:CDS 35.0%
AGTTTCTTGTCACTGACATA+TGG - tig0002988:7740-7759 Msa1339000:intergenic 35.0%
AACTTAATGTTGGAATTCGG+AGG + tig0002988:7804-7823 Msa1339000:CDS 35.0%
ACTTAATGTTGGAATTCGGA+GGG + tig0002988:7805-7824 Msa1339000:CDS 35.0%
GCTAAAAGGCGAAATAATGT+TGG + tig0002988:7827-7846 Msa1339000:CDS 35.0%
!! TGGAAATTTGGTAGTGGAAT+TGG + tig0002988:7869-7888 Msa1339000:CDS 35.0%
GTTTGCATCAGATTGTGTCA+AGG - tig0002988:7537-7556 Msa1339000:intergenic 40.0%
ACACAATCTGATGCAAACAC+TGG + tig0002988:7539-7558 Msa1339000:CDS 40.0%
!! CAAACGAGGAAAAAGCGTTT+CGG - tig0002988:7594-7613 Msa1339000:intergenic 40.0%
TTAGCTGAATAGTCCAAACG+AGG - tig0002988:7608-7627 Msa1339000:intergenic 40.0%
GATAGATGTCCTGAAAAGGA+GGG - tig0002988:7631-7650 Msa1339000:intergenic 40.0%
TTGGGATAGATGTCCTGAAA+AGG - tig0002988:7635-7654 Msa1339000:intergenic 40.0%
!! TTTTCAGGACATCTATCCCA+AGG + tig0002988:7634-7653 Msa1339000:CDS 40.0%
ATCTATCCCAAGGATGTACA+TGG + tig0002988:7644-7663 Msa1339000:CDS 40.0%
! CTTTTCACCATGTACATCCT+TGG - tig0002988:7654-7673 Msa1339000:intergenic 40.0%
AGGATGTACATGGTGAAAAG+TGG + tig0002988:7654-7673 Msa1339000:CDS 40.0%
ATGGTGAAAAGTGGCATTTC+AGG + tig0002988:7663-7682 Msa1339000:CDS 40.0%
GGCATTTCAGGCATGTTTAT+AGG + tig0002988:7675-7694 Msa1339000:CDS 40.0%
GTTGTCAACAAATGCCTCTT+CGG - tig0002988:7707-7726 Msa1339000:intergenic 40.0%
!! AAGAGGCATTTGTTGACAAC+TGG + tig0002988:7707-7726 Msa1339000:CDS 40.0%
! AGTGACAAGAAACTTGCTTC+TGG + tig0002988:7746-7765 Msa1339000:CDS 40.0%
! GTGACAAGAAACTTGCTTCT+GGG + tig0002988:7747-7766 Msa1339000:CDS 40.0%
! TGACAAGAAACTTGCTTCTG+GGG + tig0002988:7748-7767 Msa1339000:CDS 40.0%
CAATCTGATGCAAACACTGG+TGG + tig0002988:7542-7561 Msa1339000:CDS 45.0%
AGCGTTTCGGCACAATATCT+AGG - tig0002988:7581-7600 Msa1339000:intergenic 45.0%
!!! CGAAACGCTTTTTCCTCGTT+TGG + tig0002988:7592-7611 Msa1339000:CDS 45.0%
! TTCAGCTAACCCTCCTTTTC+AGG + tig0002988:7619-7638 Msa1339000:CDS 45.0%
GGATAGATGTCCTGAAAAGG+AGG - tig0002988:7632-7651 Msa1339000:intergenic 45.0%
! GGCATTTGTTGACAACTGGA+TGG + tig0002988:7711-7730 Msa1339000:CDS 45.0%
TTGGAATTCGGAGGGCTAAA+AGG + tig0002988:7813-7832 Msa1339000:CDS 45.0%
TTGGAGTCGATCCTTTGTCA+GGG + tig0002988:7846-7865 Msa1339000:CDS 45.0%
CCAAATTTCCACCCTGACAA+AGG - tig0002988:7860-7879 Msa1339000:intergenic 45.0%
CCTTTGTCAGGGTGGAAATT+TGG + tig0002988:7857-7876 Msa1339000:CDS 45.0%
TCAGGGTGGAAATTTGGTAG+TGG + tig0002988:7863-7882 Msa1339000:CDS 45.0%
ATTTGTCCTGCACCACCTTA+CGG + tig0002988:7893-7912 Msa1339000:CDS 45.0%
TATGGAAATCCTCCGTAAGG+TGG - tig0002988:7908-7927 Msa1339000:intergenic 45.0%
TCTGATGCAAACACTGGTGG+TGG + tig0002988:7545-7564 Msa1339000:CDS 50.0%
GTTGGAGTCGATCCTTTGTC+AGG + tig0002988:7845-7864 Msa1339000:CDS 50.0%
! TTTATAGGGGCACGCCGAAG+AGG + tig0002988:7690-7709 Msa1339000:CDS 55.0%
GAGTCGATCCTTTGTCAGGG+TGG + tig0002988:7849-7868 Msa1339000:CDS 55.0%
AATCCTCCGTAAGGTGGTGC+AGG - tig0002988:7902-7921 Msa1339000:intergenic 55.0%
TGTCCTGCACCACCTTACGG+AGG + tig0002988:7896-7915 Msa1339000:CDS 60.0%
Chromosome Type Strat End Strand Name
tig0002988 gene 7392 7928 7392 ID=Msa1339000;Name=Msa1339000
tig0002988 mRNA 7392 7928 7392 ID=Msa1339000-mRNA-1;Parent=Msa1339000;Name=Msa1339000-mRNA-1;_AED=0.33;_eAED=0.33;_QI=0|-1|0|1|-1|1|1|0|178
tig0002988 exon 7392 7928 7392 ID=Msa1339000-mRNA-1:exon:1914;Parent=Msa1339000-mRNA-1
tig0002988 CDS 7392 7928 7392 ID=Msa1339000-mRNA-1:cds;Parent=Msa1339000-mRNA-1
Gene Sequence

>Msa1339000

ATGGCTAATCATGAGACCGATGAAGTGTATGCAAAACTCAGGCTTGTTCCTATGAATATTAACCAAGTTAGTTTTGATAATGATGGTGTTGCTGGCATCAACGTGTCTGAAATTAAGGATAAACATCAATCTTTTGCGAAAACCTTGACACAATCTGATGCAAACACTGGTGGTGGCTTTTCTTGTCCTAGATATTGTGCCGAAACGCTTTTTCCTCGTTTGGACTATTCAGCTAACCCTCCTTTTCAGGACATCTATCCCAAGGATGTACATGGTGAAAAGTGGCATTTCAGGCATGTTTATAGGGGCACGCCGAAGAGGCATTTGTTGACAACTGGATGGAGTCCATATGTCAGTGACAAGAAACTTGCTTCTGGGGATTCAATTGTGTTTTTGAGATCTGAAAACAGTGAACTTAATGTTGGAATTCGGAGGGCTAAAAGGCGAAATAATGTTGGAGTCGATCCTTTGTCAGGGTGGAAATTTGGTAGTGGAATTGGAATTTGTCCTGCACCACCTTACGGAGGATTTCCATAG
Protein sequence

>Msa1339000

MANHETDEVYAKLRLVPMNINQVSFDNDGVAGINVSEIKDKHQSFAKTLTQSDANTGGGFSCPRYCAETLFPRLDYSANPPFQDIYPKDVHGEKWHFRHVYRGTPKRHLLTTGWSPYVSDKKLASGDSIVFLRSENSELNVGIRRAKRRNNVGVDPLSGWKFGSGIGICPAPPYGGFP*