Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1339460 | KEH15779.1 | 87.162 | 296 | 37 | 1 | 1 | 295 | 1 | 296 | 0.0 | 546 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1339460 | sp|Q9SZL8|FRS5_ARATH | 28.571 | 266 | 173 | 4 | 30 | 295 | 68 | 316 | 6.86e-26 | 110 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1339460 | A0A072TE13 | 87.162 | 296 | 37 | 1 | 1 | 295 | 1 | 296 | 0.0 | 546 |
| Gene ID | Type | Classification |
|---|---|---|
| Msa1339460 | TF | FAR1 |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1339460 | MtrunA17_Chr1g0172971 | 82.051 | 117 | 20 | 1 | 1 | 116 | 1 | 117 | 6.79e-59 | 197 |
| Msa1339460 | MtrunA17_Chr6g0475591 | 89.041 | 73 | 8 | 0 | 188 | 260 | 1 | 73 | 2.20e-42 | 140 |
| Msa1339460 | MtrunA17_Chr7g0258861 | 31.559 | 263 | 158 | 5 | 36 | 295 | 8 | 251 | 1.14e-38 | 138 |
| Msa1339460 | MtrunA17_Chr2g0324011 | 29.658 | 263 | 162 | 4 | 36 | 295 | 8 | 250 | 9.02e-33 | 127 |
| Msa1339460 | MtrunA17_Chr1g0202341 | 31.559 | 263 | 156 | 6 | 34 | 294 | 50 | 290 | 6.71e-31 | 122 |
| Msa1339460 | MtrunA17_Chr3g0082121 | 30.588 | 255 | 150 | 6 | 41 | 295 | 49 | 276 | 1.77e-29 | 117 |
| Msa1339460 | MtrunA17_Chr1g0164591 | 32.727 | 165 | 102 | 2 | 131 | 295 | 1 | 156 | 1.13e-24 | 101 |
| Msa1339460 | MtrunA17_Chr5g0448761 | 26.966 | 267 | 176 | 4 | 32 | 295 | 57 | 307 | 1.31e-24 | 103 |
| Msa1339460 | MtrunA17_Chr8g0363841 | 29.762 | 252 | 152 | 7 | 40 | 288 | 22 | 251 | 2.63e-24 | 102 |
| Msa1339460 | MtrunA17_Chr1g0203721 | 26.437 | 261 | 163 | 6 | 40 | 294 | 68 | 305 | 2.92e-22 | 97.1 |
| Msa1339460 | MtrunA17_Chr2g0296781 | 27.413 | 259 | 160 | 6 | 39 | 295 | 1 | 233 | 1.51e-21 | 94.7 |
| Msa1339460 | MtrunA17_Chr2g0287651 | 26.871 | 294 | 183 | 9 | 9 | 294 | 48 | 317 | 9.35e-19 | 86.7 |
| Msa1339460 | MtrunA17_Chr6g0458391 | 27.519 | 258 | 144 | 6 | 41 | 295 | 231 | 448 | 3.46e-18 | 84.7 |
| Msa1339460 | MtrunA17_Chr5g0441581 | 26.217 | 267 | 163 | 8 | 32 | 295 | 26 | 261 | 1.16e-17 | 83.2 |
| Msa1339460 | MtrunA17_Chr5g0431001 | 26.515 | 264 | 169 | 8 | 40 | 295 | 66 | 312 | 1.28e-16 | 79.7 |
| Msa1339460 | MtrunA17_Chr1g0203051 | 23.482 | 247 | 166 | 4 | 50 | 294 | 94 | 319 | 9.40e-16 | 77.4 |
| Msa1339460 | MtrunA17_Chr1g0198761 | 25.357 | 280 | 180 | 8 | 26 | 295 | 38 | 298 | 9.61e-16 | 77.4 |
| Msa1339460 | MtrunA17_Chr1g0196541 | 25.869 | 259 | 162 | 8 | 39 | 294 | 1 | 232 | 1.14e-15 | 77.4 |
| Msa1339460 | MtrunA17_Chr2g0276841 | 24.206 | 252 | 167 | 7 | 49 | 295 | 84 | 316 | 3.41e-15 | 75.9 |
| Msa1339460 | MtrunA17_Chr2g0291591 | 24.232 | 293 | 171 | 11 | 16 | 294 | 44 | 299 | 1.52e-14 | 73.6 |
| Msa1339460 | MtrunA17_Chr6g0477261 | 31.034 | 116 | 80 | 0 | 180 | 295 | 7 | 122 | 2.77e-14 | 73.2 |
| Msa1339460 | MtrunA17_Chr2g0294841 | 25.581 | 258 | 159 | 10 | 41 | 287 | 51 | 286 | 1.11e-13 | 71.2 |
| Msa1339460 | MtrunA17_Chr5g0440451 | 22.426 | 272 | 162 | 7 | 34 | 288 | 42 | 281 | 6.55e-13 | 68.9 |
| Msa1339460 | MtrunA17_Chr2g0329031 | 24.231 | 260 | 156 | 8 | 41 | 294 | 20 | 244 | 8.74e-13 | 68.6 |
| Msa1339460 | MtrunA17_Chr1g0207551 | 23.221 | 267 | 179 | 6 | 31 | 295 | 30 | 272 | 2.08e-11 | 64.3 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1339460 | AT4G38180.1 | 28.571 | 266 | 173 | 4 | 30 | 295 | 68 | 316 | 6.97e-27 | 110 |
| Msa1339460 | AT5G28530.3 | 23.462 | 260 | 169 | 6 | 40 | 287 | 60 | 301 | 7.29e-16 | 78.2 |
| Msa1339460 | AT5G28530.2 | 23.462 | 260 | 169 | 6 | 40 | 287 | 60 | 301 | 7.29e-16 | 78.2 |
| Msa1339460 | AT5G28530.1 | 23.462 | 260 | 169 | 6 | 40 | 287 | 60 | 301 | 7.94e-16 | 78.2 |
| Msa1339460 | AT1G10240.2 | 24.335 | 263 | 171 | 8 | 40 | 295 | 53 | 294 | 4.05e-15 | 75.9 |
| Msa1339460 | AT1G10240.1 | 24.335 | 263 | 171 | 8 | 40 | 295 | 53 | 294 | 4.05e-15 | 75.9 |
| Msa1339460 | AT4G15090.1 | 25.506 | 247 | 163 | 6 | 50 | 295 | 66 | 292 | 4.43e-12 | 67.0 |
| Msa1339460 | AT4G15090.5 | 25.506 | 247 | 163 | 6 | 50 | 295 | 66 | 292 | 4.43e-12 | 67.0 |
| Msa1339460 | AT4G15090.3 | 25.506 | 247 | 163 | 6 | 50 | 295 | 66 | 292 | 4.43e-12 | 67.0 |
| Msa1339460 | AT4G15090.2 | 25.506 | 247 | 163 | 6 | 50 | 295 | 66 | 292 | 4.43e-12 | 67.0 |
| Msa1339460 | AT4G15090.4 | 25.506 | 247 | 163 | 6 | 50 | 295 | 66 | 292 | 4.43e-12 | 67.0 |
| Msa1339460 | AT1G76320.3 | 23.695 | 249 | 154 | 8 | 50 | 294 | 12 | 228 | 2.28e-11 | 64.7 |
| Msa1339460 | AT1G76320.1 | 22.267 | 247 | 160 | 6 | 50 | 294 | 12 | 228 | 2.83e-11 | 64.3 |
| Msa1339460 | AT1G76320.4 | 22.267 | 247 | 160 | 6 | 50 | 294 | 12 | 228 | 2.83e-11 | 64.3 |
| Msa1339460 | AT1G76320.2 | 22.267 | 247 | 160 | 6 | 50 | 294 | 12 | 228 | 2.93e-11 | 64.3 |
| Msa1339460 | AT2G27110.2 | 26.316 | 171 | 109 | 4 | 127 | 295 | 108 | 263 | 4.51e-11 | 63.9 |
| Msa1339460 | AT2G27110.1 | 26.316 | 171 | 109 | 4 | 127 | 295 | 108 | 263 | 4.51e-11 | 63.9 |
Find 45 sgRNAs with CRISPR-Local
Find 56 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CAACAAGATTATGCTCTATT+TGG | 0.198611 | tig0003325:-14728 | Msa1339460:CDS |
| GCCGATATTTATTGTTTGTT+CGG | 0.341758 | tig0003325:+14678 | None:intergenic |
| AACAATAAATATCGGCCATT+AGG | 0.388497 | tig0003325:-14671 | Msa1339460:CDS |
| ACCGAACAAACAATAAATAT+CGG | 0.389395 | tig0003325:-14679 | Msa1339460:CDS |
| TTCTCATACTCAACAAGAAC+TGG | 0.395633 | tig0003325:+15208 | None:intergenic |
| TAAAGAATCATGGAAGTCTT+TGG | 0.408500 | tig0003325:-15437 | Msa1339460:CDS |
| ATAACACTGGATTCTCTGTT+AGG | 0.417808 | tig0003325:-15366 | Msa1339460:CDS |
| CGAGAAGTAAATGAAGTTCA+AGG | 0.430014 | tig0003325:-15031 | Msa1339460:CDS |
| AGCTGGAATATATTTGAAGT+TGG | 0.433136 | tig0003325:-15149 | Msa1339460:CDS |
| TGAATGTCTATAAGATATAT+AGG | 0.445219 | tig0003325:-15117 | Msa1339460:CDS |
| GCTCTATTTGGTGATGTTGT+CGG | 0.445253 | tig0003325:-14716 | Msa1339460:CDS |
| GAAAGGGTTGGTTGTAAAGC+TGG | 0.447509 | tig0003325:-15166 | Msa1339460:CDS |
| CGTTATGCTTGTAACAAAGA+AGG | 0.449439 | tig0003325:-15283 | Msa1339460:CDS |
| GCTTTAATAAATTCTAAAGC+TGG | 0.450264 | tig0003325:-15001 | Msa1339460:CDS |
| CTCAAGTAGCGCAAATGCTC+TGG | 0.460440 | tig0003325:+15061 | None:intergenic |
| ACAAAATAAGAAATCGGATA+AGG | 0.469178 | tig0003325:-15317 | Msa1339460:CDS |
| AGTACAAAATACTAACCTAA+TGG | 0.480025 | tig0003325:+14656 | None:intergenic |
| GAAGTGATGGGTCAAGGAGT+TGG | 0.484292 | tig0003325:-14956 | Msa1339460:CDS |
| TTACAACCAACCCTTTCCTC+TGG | 0.485330 | tig0003325:+15172 | None:intergenic |
| TTTAATTATGCATATAACAC+TGG | 0.485542 | tig0003325:-15379 | Msa1339460:CDS |
| TCTAATGACAACTCGTCAAA+AGG | 0.488137 | tig0003325:-14888 | Msa1339460:CDS |
| CAATTCACAAAATAAGAAAT+CGG | 0.490263 | tig0003325:-15323 | Msa1339460:CDS |
| CGTCAAAAGGAGATGGTGGC+TGG | 0.492100 | tig0003325:-14875 | Msa1339460:CDS |
| GAGAACATCTTATGAAGTGA+TGG | 0.499875 | tig0003325:-14969 | Msa1339460:CDS |
| TGTCTTCTGCAGCATCAACT+TGG | 0.502977 | tig0003325:+14783 | None:intergenic |
| CCATGATTCTTTATCACTCA+AGG | 0.503274 | tig0003325:+15447 | None:intergenic |
| TTTAAGTCCGAAAATCGAAA+AGG | 0.508080 | tig0003325:+14917 | None:intergenic |
| TGTCTATAAGATATATAGGT+GGG | 0.523740 | tig0003325:-15113 | Msa1339460:CDS |
| CCTTGAGTGATAAAGAATCA+TGG | 0.524357 | tig0003325:-15447 | Msa1339460:CDS |
| TAACAAAGAAGGTTTCAAGA+AGG | 0.538422 | tig0003325:-15272 | Msa1339460:CDS |
| CACTTCATAAGATGTTCTCA+TGG | 0.541827 | tig0003325:+14973 | None:intergenic |
| AACAAAGAAGGTTTCAAGAA+GGG | 0.545537 | tig0003325:-15271 | Msa1339460:CDS |
| AAATGCTCTGGTTTATGTAA+TGG | 0.552634 | tig0003325:+15073 | None:intergenic |
| TGAAAATTCAGTTATTGATG+CGG | 0.565546 | tig0003325:-15488 | Msa1339460:CDS |
| ATCTGAGAGTGGACAACTGA+CGG | 0.566425 | tig0003325:-15512 | Msa1339460:CDS |
| GTGATGGGTCAAGGAGTTGG+AGG | 0.568802 | tig0003325:-14953 | Msa1339460:CDS |
| TCTTATGAAGTGATGGGTCA+AGG | 0.589029 | tig0003325:-14962 | Msa1339460:CDS |
| GACAACTCGTCAAAAGGAGA+TGG | 0.599038 | tig0003325:-14882 | Msa1339460:CDS |
| AGAACATCTTATGAAGTGAT+GGG | 0.601988 | tig0003325:-14968 | Msa1339460:CDS |
| AACTCGTCAAAAGGAGATGG+TGG | 0.603142 | tig0003325:-14879 | Msa1339460:CDS |
| ATATCAACTTCATCTGAGAG+TGG | 0.614980 | tig0003325:-15523 | Msa1339460:CDS |
| AATGAAGCTATCTCGAAACC+AGG | 0.620625 | tig0003325:-14821 | Msa1339460:CDS |
| AGAACTGGACTGTCACATGT+TGG | 0.620791 | tig0003325:+15223 | None:intergenic |
| GGGTCAAGGAGTTGGAGGCA+CGG | 0.621882 | tig0003325:-14948 | Msa1339460:CDS |
| ATGTCTATAAGATATATAGG+TGG | 0.643489 | tig0003325:-15114 | Msa1339460:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TTTAATTATGCATATAACAC+TGG | - | tig0003325:14820-14839 | Msa1339460:CDS | 20.0% |
| !! | CAATTCACAAAATAAGAAAT+CGG | - | tig0003325:14876-14895 | Msa1339460:CDS | 20.0% |
| !! | TGAATGTCTATAAGATATAT+AGG | - | tig0003325:15082-15101 | Msa1339460:CDS | 20.0% |
| ! | TGAAAATTCAGTTATTGATG+CGG | - | tig0003325:14711-14730 | Msa1339460:CDS | 25.0% |
| !!! | CTTATTTTGTGAATTGTTCT+TGG | + | tig0003325:14872-14891 | None:intergenic | 25.0% |
| ! | ACAAAATAAGAAATCGGATA+AGG | - | tig0003325:14882-14901 | Msa1339460:CDS | 25.0% |
| ! | ATGTCTATAAGATATATAGG+TGG | - | tig0003325:15085-15104 | Msa1339460:CDS | 25.0% |
| ! | TGTCTATAAGATATATAGGT+GGG | - | tig0003325:15086-15105 | Msa1339460:CDS | 25.0% |
| !!! | GCTTTAATAAATTCTAAAGC+TGG | - | tig0003325:15198-15217 | Msa1339460:CDS | 25.0% |
| !! | GAATTTTCCTTTTCGATTTT+CGG | - | tig0003325:15275-15294 | Msa1339460:CDS | 25.0% |
| !!! | AAGACATAGCTAGTATTTTT+TGG | - | tig0003325:15433-15452 | Msa1339460:CDS | 25.0% |
| !!! | AGACATAGCTAGTATTTTTT+GGG | - | tig0003325:15434-15453 | Msa1339460:CDS | 25.0% |
| ! | ACCGAACAAACAATAAATAT+CGG | - | tig0003325:15520-15539 | Msa1339460:CDS | 25.0% |
| TAAAGAATCATGGAAGTCTT+TGG | - | tig0003325:14762-14781 | Msa1339460:CDS | 30.0% | |
| TAACAAAGAAGGTTTCAAGA+AGG | - | tig0003325:14927-14946 | Msa1339460:CDS | 30.0% | |
| AACAAAGAAGGTTTCAAGAA+GGG | - | tig0003325:14928-14947 | Msa1339460:CDS | 30.0% | |
| TAAAGAAAAACCAGAGGAAA+GGG | - | tig0003325:15017-15036 | Msa1339460:CDS | 30.0% | |
| ! | AGCTGGAATATATTTGAAGT+TGG | - | tig0003325:15050-15069 | Msa1339460:CDS | 30.0% |
| ! | AAATGCTCTGGTTTATGTAA+TGG | + | tig0003325:15129-15148 | None:intergenic | 30.0% |
| AGAACATCTTATGAAGTGAT+GGG | - | tig0003325:15231-15250 | Msa1339460:CDS | 30.0% | |
| TTTAAGTCCGAAAATCGAAA+AGG | + | tig0003325:15285-15304 | None:intergenic | 30.0% | |
| TGGATATCATAGTAAAAACC+TGG | + | tig0003325:15399-15418 | None:intergenic | 30.0% | |
| CAACAAGATTATGCTCTATT+TGG | - | tig0003325:15471-15490 | Msa1339460:CDS | 30.0% | |
| GCCGATATTTATTGTTTGTT+CGG | + | tig0003325:15524-15543 | None:intergenic | 30.0% | |
| AACAATAAATATCGGCCATT+AGG | - | tig0003325:15528-15547 | Msa1339460:CDS | 30.0% | |
| ATATCAACTTCATCTGAGAG+TGG | - | tig0003325:14676-14695 | Msa1339460:CDS | 35.0% | |
| CCATGATTCTTTATCACTCA+AGG | + | tig0003325:14755-14774 | None:intergenic | 35.0% | |
| CCTTGAGTGATAAAGAATCA+TGG | - | tig0003325:14752-14771 | Msa1339460:CDS | 35.0% | |
| ATAACACTGGATTCTCTGTT+AGG | - | tig0003325:14833-14852 | Msa1339460:CDS | 35.0% | |
| CGTTATGCTTGTAACAAAGA+AGG | - | tig0003325:14916-14935 | Msa1339460:CDS | 35.0% | |
| GTCTGCTTAATCCTAAAAAC+AGG | - | tig0003325:14953-14972 | Msa1339460:CDS | 35.0% | |
| TTCTCATACTCAACAAGAAC+TGG | + | tig0003325:14994-15013 | None:intergenic | 35.0% | |
| GAAAGCTAAAGAAAAACCAG+AGG | - | tig0003325:15011-15030 | Msa1339460:CDS | 35.0% | |
| CTAAAGAAAAACCAGAGGAA+AGG | - | tig0003325:15016-15035 | Msa1339460:CDS | 35.0% | |
| CGAGAAGTAAATGAAGTTCA+AGG | - | tig0003325:15168-15187 | Msa1339460:CDS | 35.0% | |
| CACTTCATAAGATGTTCTCA+TGG | + | tig0003325:15229-15248 | None:intergenic | 35.0% | |
| GAGAACATCTTATGAAGTGA+TGG | - | tig0003325:15230-15249 | Msa1339460:CDS | 35.0% | |
| TCTAATGACAACTCGTCAAA+AGG | - | tig0003325:15311-15330 | Msa1339460:CDS | 35.0% | |
| !! | TCACATGTTGGCCTGTTTTT+AGG | + | tig0003325:14967-14986 | None:intergenic | 40.0% |
| !! | TCTTATGAAGTGATGGGTCA+AGG | - | tig0003325:15237-15256 | Msa1339460:CDS | 40.0% |
| AATGAAGCTATCTCGAAACC+AGG | - | tig0003325:15378-15397 | Msa1339460:CDS | 40.0% | |
| !!! | GCTAGTATTTTTTGGGCAGA+TGG | - | tig0003325:15441-15460 | Msa1339460:CDS | 40.0% |
| !! | GCTCTATTTGGTGATGTTGT+CGG | - | tig0003325:15483-15502 | Msa1339460:CDS | 40.0% |
| ATCTGAGAGTGGACAACTGA+CGG | - | tig0003325:14687-14706 | Msa1339460:CDS | 45.0% | |
| AGAACTGGACTGTCACATGT+TGG | + | tig0003325:14979-14998 | None:intergenic | 45.0% | |
| GAAAAACCAGAGGAAAGGGT+TGG | - | tig0003325:15021-15040 | Msa1339460:CDS | 45.0% | |
| TTACAACCAACCCTTTCCTC+TGG | + | tig0003325:15030-15049 | None:intergenic | 45.0% | |
| !! | GAAAGGGTTGGTTGTAAAGC+TGG | - | tig0003325:15033-15052 | Msa1339460:CDS | 45.0% |
| GACAACTCGTCAAAAGGAGA+TGG | - | tig0003325:15317-15336 | Msa1339460:CDS | 45.0% | |
| AACTCGTCAAAAGGAGATGG+TGG | - | tig0003325:15320-15339 | Msa1339460:CDS | 45.0% | |
| TGTCTTCTGCAGCATCAACT+TGG | + | tig0003325:15419-15438 | None:intergenic | 45.0% | |
| CTCAAGTAGCGCAAATGCTC+TGG | + | tig0003325:15141-15160 | None:intergenic | 50.0% | |
| !! | GAAGTGATGGGTCAAGGAGT+TGG | - | tig0003325:15243-15262 | Msa1339460:CDS | 50.0% |
| !! | GTGATGGGTCAAGGAGTTGG+AGG | - | tig0003325:15246-15265 | Msa1339460:CDS | 55.0% |
| ! | CGTCAAAAGGAGATGGTGGC+TGG | - | tig0003325:15324-15343 | Msa1339460:CDS | 55.0% |
| GGGTCAAGGAGTTGGAGGCA+CGG | - | tig0003325:15251-15270 | Msa1339460:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| tig0003325 | gene | 14667 | 15554 | 14667 | ID=Msa1339460;Name=Msa1339460 |
| tig0003325 | mRNA | 14667 | 15554 | 14667 | ID=Msa1339460-mRNA-1;Parent=Msa1339460;Name=Msa1339460-mRNA-1;_AED=0.00;_eAED=0.00;_QI=0|-1|0|1|-1|1|1|0|295 |
| tig0003325 | exon | 14667 | 15554 | 14667 | ID=Msa1339460-mRNA-1:exon:2135;Parent=Msa1339460-mRNA-1 |
| tig0003325 | CDS | 14667 | 15554 | 14667 | ID=Msa1339460-mRNA-1:cds;Parent=Msa1339460-mRNA-1 |
| Gene Sequence |
| Protein sequence |
