Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1340990 | RHN82063.1 | 52.838 | 229 | 52 | 3 | 99 | 325 | 5 | 179 | 5.13e-60 | 200 |
| Msa1340990 | RHN82063.1 | 77.660 | 188 | 27 | 2 | 13 | 200 | 2 | 174 | 3.36e-90 | 277 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1340990 | sp|Q9XIK6|SNL6_ARATH | 34.194 | 155 | 93 | 3 | 9 | 158 | 80 | 230 | 1.83e-15 | 80.9 |
| Msa1340990 | sp|Q9XIK6|SNL6_ARATH | 31.765 | 170 | 89 | 3 | 99 | 267 | 87 | 230 | 1.74e-11 | 68.9 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1340990 | A0A396K1D1 | 52.838 | 229 | 52 | 3 | 99 | 325 | 5 | 179 | 2.45e-60 | 200 |
| Msa1340990 | A0A396K1D1 | 77.660 | 188 | 27 | 2 | 13 | 200 | 2 | 174 | 1.61e-90 | 277 |
| Gene ID | Type | Classification |
|---|---|---|
| Msa1340990 | TR | Others |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1340990 | MtrunA17_Chr1g0205831 | 77.660 | 188 | 27 | 2 | 13 | 200 | 2 | 174 | 3.09e-94 | 277 |
| Msa1340990 | MtrunA17_Chr1g0205831 | 52.838 | 229 | 52 | 3 | 99 | 325 | 5 | 179 | 4.72e-64 | 200 |
| Msa1340990 | MtrunA17_Chr1g0205821 | 60.000 | 185 | 47 | 2 | 1 | 170 | 1 | 173 | 2.91e-65 | 203 |
| Msa1340990 | MtrunA17_Chr1g0205821 | 55.682 | 176 | 55 | 1 | 104 | 279 | 21 | 173 | 1.97e-57 | 183 |
| Msa1340990 | MtrunA17_Chr1g0205821 | 51.807 | 83 | 40 | 0 | 202 | 284 | 10 | 92 | 8.52e-20 | 85.1 |
| Msa1340990 | MtrunA17_Chr4g0075861 | 38.489 | 278 | 136 | 6 | 1 | 277 | 1 | 244 | 3.75e-40 | 144 |
| Msa1340990 | MtrunA17_Chr4g0075861 | 38.889 | 180 | 80 | 5 | 113 | 292 | 26 | 175 | 7.73e-20 | 89.0 |
| Msa1340990 | MtrunA17_Chr4g0075971 | 40.143 | 279 | 130 | 8 | 1 | 277 | 1 | 244 | 2.77e-39 | 146 |
| Msa1340990 | MtrunA17_Chr4g0075971 | 41.436 | 181 | 74 | 7 | 113 | 292 | 26 | 175 | 2.51e-20 | 91.7 |
| Msa1340990 | MtrunA17_Chr7g0218891 | 31.271 | 291 | 160 | 7 | 1 | 280 | 1 | 262 | 1.91e-30 | 116 |
| Msa1340990 | MtrunA17_Chr7g0218891 | 32.323 | 198 | 103 | 5 | 100 | 293 | 17 | 187 | 9.42e-17 | 79.0 |
| Msa1340990 | MtrunA17_Chr1g0205811 | 56.436 | 101 | 43 | 1 | 99 | 199 | 17 | 116 | 3.50e-29 | 108 |
| Msa1340990 | MtrunA17_Chr1g0205811 | 50.847 | 118 | 46 | 2 | 208 | 325 | 17 | 122 | 4.90e-29 | 108 |
| Msa1340990 | MtrunA17_Chr1g0205811 | 61.250 | 80 | 29 | 1 | 5 | 82 | 4 | 83 | 1.99e-25 | 98.6 |
| Msa1340990 | MtrunA17_Chr1g0206771 | 39.634 | 164 | 93 | 3 | 2 | 164 | 5 | 163 | 1.90e-28 | 108 |
| Msa1340990 | MtrunA17_Chr1g0206771 | 33.714 | 175 | 86 | 1 | 99 | 273 | 19 | 163 | 3.28e-25 | 100 |
| Msa1340990 | MtrunA17_Chr3g0077891 | 40.828 | 169 | 91 | 3 | 11 | 170 | 6 | 174 | 2.60e-28 | 108 |
| Msa1340990 | MtrunA17_Chr3g0077891 | 36.898 | 187 | 89 | 4 | 99 | 279 | 11 | 174 | 7.22e-23 | 93.6 |
| Msa1340990 | MtrunA17_Chr3g0077941 | 40.476 | 168 | 92 | 3 | 11 | 170 | 6 | 173 | 1.09e-26 | 103 |
| Msa1340990 | MtrunA17_Chr3g0077941 | 36.066 | 183 | 95 | 4 | 99 | 279 | 11 | 173 | 1.38e-18 | 82.0 |
| Msa1340990 | MtrunA17_Chr1g0205801 | 59.740 | 77 | 31 | 0 | 6 | 82 | 5 | 81 | 1.28e-25 | 99.0 |
| Msa1340990 | MtrunA17_Chr1g0205801 | 53.535 | 99 | 38 | 2 | 99 | 190 | 15 | 112 | 1.80e-23 | 93.2 |
| Msa1340990 | MtrunA17_Chr1g0205801 | 55.172 | 87 | 38 | 1 | 208 | 294 | 15 | 100 | 6.31e-23 | 91.7 |
| Msa1340990 | MtrunA17_Chr3g0077881 | 40.588 | 170 | 83 | 5 | 11 | 167 | 6 | 170 | 1.08e-24 | 98.6 |
| Msa1340990 | MtrunA17_Chr3g0077881 | 36.413 | 184 | 87 | 5 | 99 | 276 | 11 | 170 | 4.04e-20 | 86.3 |
| Msa1340990 | MtrunA17_Chr3g0077911 | 38.418 | 177 | 81 | 4 | 99 | 274 | 11 | 160 | 1.40e-23 | 95.1 |
| Msa1340990 | MtrunA17_Chr3g0077911 | 42.208 | 154 | 75 | 4 | 12 | 158 | 7 | 153 | 1.21e-22 | 92.4 |
| Msa1340990 | MtrunA17_Chr3g0077911 | 42.353 | 85 | 45 | 2 | 208 | 292 | 11 | 91 | 1.68e-13 | 67.4 |
| Msa1340990 | MtrunA17_Chr3g0077861 | 39.752 | 161 | 83 | 4 | 12 | 165 | 7 | 160 | 1.26e-20 | 87.4 |
| Msa1340990 | MtrunA17_Chr3g0077861 | 37.195 | 164 | 78 | 3 | 112 | 274 | 21 | 160 | 2.58e-20 | 86.3 |
| Msa1340990 | MtrunA17_Chr5g0415031 | 35.484 | 155 | 89 | 4 | 11 | 158 | 41 | 191 | 1.01e-17 | 84.3 |
| Msa1340990 | MtrunA17_Chr5g0415031 | 29.891 | 184 | 103 | 2 | 87 | 267 | 31 | 191 | 4.65e-17 | 82.4 |
| Msa1340990 | MtrunA17_Chr5g0415031 | 35.780 | 109 | 65 | 1 | 208 | 311 | 46 | 154 | 4.62e-12 | 67.0 |
| Msa1340990 | MtrunA17_Chr1g0206781 | 43.158 | 95 | 49 | 1 | 191 | 285 | 2 | 91 | 9.66e-17 | 75.1 |
| Msa1340990 | MtrunA17_Chr1g0206781 | 44.872 | 78 | 43 | 0 | 99 | 176 | 14 | 91 | 4.93e-15 | 70.5 |
| Msa1340990 | MtrunA17_Chr1g0206781 | 35.878 | 131 | 59 | 3 | 1 | 131 | 1 | 106 | 1.51e-13 | 66.6 |
| Msa1340990 | MtrunA17_Chr8g0364231 | 35.897 | 156 | 87 | 6 | 11 | 158 | 40 | 190 | 8.65e-16 | 78.6 |
| Msa1340990 | MtrunA17_Chr8g0364231 | 33.728 | 169 | 89 | 2 | 99 | 267 | 45 | 190 | 1.44e-15 | 77.8 |
| Msa1340990 | MtrunA17_Chr8g0364231 | 50.000 | 74 | 36 | 1 | 208 | 281 | 45 | 117 | 2.96e-13 | 70.9 |
| Msa1340990 | MtrunA17_Chr1g0209201 | 40.625 | 128 | 66 | 3 | 4 | 131 | 10 | 127 | 1.32e-15 | 72.8 |
| Msa1340990 | MtrunA17_Chr1g0209201 | 34.307 | 137 | 57 | 3 | 104 | 240 | 24 | 127 | 9.60e-12 | 62.0 |
| Msa1340990 | MtrunA17_Chr6g0452001 | 35.065 | 154 | 91 | 3 | 11 | 158 | 61 | 211 | 1.99e-15 | 77.4 |
| Msa1340990 | MtrunA17_Chr6g0452001 | 29.586 | 169 | 96 | 1 | 99 | 267 | 66 | 211 | 7.18e-12 | 66.6 |
| Msa1340990 | MtrunA17_Chr6g0452021 | 34.667 | 150 | 91 | 3 | 13 | 158 | 43 | 189 | 3.84e-15 | 75.9 |
| Msa1340990 | MtrunA17_Chr1g0205861 | 52.381 | 63 | 30 | 0 | 13 | 75 | 16 | 78 | 1.28e-14 | 69.3 |
| Msa1340990 | MtrunA17_Chr8g0364021 | 44.286 | 70 | 39 | 0 | 13 | 82 | 44 | 113 | 7.57e-12 | 64.3 |
| Msa1340990 | MtrunA17_Chr3g0077811 | 46.053 | 76 | 40 | 1 | 8 | 82 | 8 | 83 | 2.54e-11 | 60.1 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1340990 | AT1G27260.1 | 37.572 | 173 | 89 | 2 | 99 | 271 | 6 | 159 | 4.32e-25 | 101 |
| Msa1340990 | AT1G27260.1 | 35.519 | 183 | 103 | 3 | 11 | 183 | 1 | 178 | 2.34e-23 | 96.7 |
| Msa1340990 | AT1G27260.1 | 45.070 | 71 | 39 | 0 | 208 | 278 | 6 | 76 | 9.90e-11 | 61.2 |
| Msa1340990 | AT1G24230.1 | 36.025 | 161 | 90 | 4 | 11 | 170 | 12 | 160 | 1.24e-21 | 92.4 |
| Msa1340990 | AT1G23810.1 | 31.507 | 219 | 133 | 4 | 99 | 316 | 17 | 219 | 8.16e-21 | 90.1 |
| Msa1340990 | AT1G23810.1 | 28.261 | 184 | 110 | 5 | 16 | 183 | 17 | 194 | 2.49e-13 | 68.9 |
| Msa1340990 | AT1G24250.1 | 32.127 | 221 | 140 | 6 | 99 | 316 | 17 | 230 | 6.30e-20 | 87.8 |
| Msa1340990 | AT1G24250.1 | 28.718 | 195 | 106 | 6 | 16 | 183 | 17 | 205 | 4.34e-13 | 68.6 |
| Msa1340990 | AT1G10450.2 | 34.194 | 155 | 93 | 3 | 9 | 158 | 29 | 179 | 1.71e-16 | 80.9 |
| Msa1340990 | AT1G10450.2 | 31.765 | 170 | 89 | 3 | 99 | 267 | 36 | 179 | 1.64e-12 | 68.9 |
| Msa1340990 | AT1G10450.1 | 34.194 | 155 | 93 | 3 | 9 | 158 | 29 | 179 | 1.71e-16 | 80.9 |
| Msa1340990 | AT1G10450.1 | 31.765 | 170 | 89 | 3 | 99 | 267 | 36 | 179 | 1.64e-12 | 68.9 |
| Msa1340990 | AT1G10450.3 | 34.194 | 155 | 93 | 3 | 9 | 158 | 80 | 230 | 1.86e-16 | 80.9 |
| Msa1340990 | AT1G10450.3 | 31.765 | 170 | 89 | 3 | 99 | 267 | 87 | 230 | 1.76e-12 | 68.9 |
| Msa1340990 | AT5G15020.1 | 35.948 | 153 | 85 | 5 | 13 | 158 | 52 | 198 | 1.11e-15 | 78.6 |
| Msa1340990 | AT5G15020.1 | 28.421 | 190 | 108 | 3 | 99 | 285 | 55 | 219 | 7.38e-11 | 63.9 |
| Msa1340990 | AT5G15020.2 | 35.948 | 153 | 85 | 5 | 13 | 158 | 52 | 198 | 1.17e-15 | 78.6 |
| Msa1340990 | AT5G15020.2 | 28.421 | 190 | 108 | 3 | 99 | 285 | 55 | 219 | 7.58e-11 | 63.9 |
| Msa1340990 | AT1G24190.1 | 34.940 | 166 | 92 | 6 | 11 | 166 | 12 | 171 | 1.57e-15 | 78.2 |
| Msa1340990 | AT1G24190.1 | 30.556 | 180 | 97 | 6 | 99 | 275 | 17 | 171 | 1.03e-11 | 66.6 |
| Msa1340990 | AT1G24190.3 | 34.940 | 166 | 92 | 6 | 11 | 166 | 12 | 171 | 1.59e-15 | 78.2 |
| Msa1340990 | AT1G24190.3 | 30.556 | 180 | 97 | 6 | 99 | 275 | 17 | 171 | 1.18e-11 | 66.2 |
| Msa1340990 | AT1G24190.2 | 34.940 | 166 | 92 | 6 | 11 | 166 | 12 | 171 | 1.60e-15 | 78.2 |
| Msa1340990 | AT1G24190.2 | 30.556 | 180 | 97 | 6 | 99 | 275 | 17 | 171 | 1.10e-11 | 66.2 |
| Msa1340990 | AT3G01320.1 | 33.918 | 171 | 97 | 6 | 11 | 174 | 55 | 216 | 2.26e-15 | 77.8 |
| Msa1340990 | AT3G01320.1 | 28.108 | 185 | 105 | 3 | 99 | 283 | 60 | 216 | 5.74e-11 | 64.3 |
| Msa1340990 | AT3G01320.2 | 33.918 | 171 | 97 | 6 | 11 | 174 | 55 | 216 | 2.86e-15 | 77.4 |
| Msa1340990 | AT3G01320.2 | 28.108 | 185 | 105 | 3 | 99 | 283 | 60 | 216 | 5.63e-11 | 64.3 |
| Msa1340990 | AT1G70060.2 | 35.484 | 155 | 90 | 4 | 11 | 158 | 12 | 163 | 1.36e-14 | 75.1 |
| Msa1340990 | AT1G70060.2 | 30.178 | 169 | 96 | 2 | 99 | 267 | 17 | 163 | 9.83e-12 | 66.6 |
| Msa1340990 | AT1G70060.1 | 35.484 | 155 | 90 | 4 | 11 | 158 | 12 | 163 | 1.36e-14 | 75.1 |
| Msa1340990 | AT1G70060.1 | 30.178 | 169 | 96 | 2 | 99 | 267 | 17 | 163 | 9.83e-12 | 66.6 |
| Msa1340990 | AT1G24200.1 | 32.515 | 163 | 86 | 6 | 9 | 159 | 10 | 160 | 7.52e-13 | 67.0 |
| Msa1340990 | AT1G24200.1 | 44.578 | 83 | 43 | 2 | 191 | 273 | 3 | 82 | 4.97e-12 | 64.3 |
| Msa1340990 | AT1G59890.4 | 34.416 | 154 | 86 | 4 | 13 | 158 | 45 | 191 | 7.62e-13 | 70.1 |
| Msa1340990 | AT1G59890.2 | 34.416 | 154 | 86 | 4 | 13 | 158 | 45 | 191 | 7.79e-13 | 69.7 |
| Msa1340990 | AT1G59890.3 | 34.416 | 154 | 86 | 4 | 13 | 158 | 45 | 191 | 8.59e-13 | 69.7 |
| Msa1340990 | AT1G59890.1 | 34.416 | 154 | 86 | 4 | 13 | 158 | 45 | 191 | 8.60e-13 | 69.7 |
| Msa1340990 | AT1G27280.1 | 31.646 | 158 | 103 | 2 | 24 | 176 | 4 | 161 | 1.50e-12 | 66.6 |
| Msa1340990 | AT1G27280.1 | 30.168 | 179 | 104 | 4 | 107 | 285 | 4 | 161 | 1.37e-11 | 63.9 |
| Msa1340990 | AT5G15025.1 | 45.714 | 70 | 38 | 0 | 13 | 82 | 47 | 116 | 2.41e-12 | 63.5 |
| Msa1340990 | AT1G27220.1 | 40.260 | 77 | 46 | 0 | 5 | 81 | 6 | 82 | 3.43e-12 | 64.7 |
| Msa1340990 | AT5G15040.1 | 41.791 | 67 | 39 | 0 | 99 | 165 | 6 | 72 | 1.19e-11 | 60.5 |
| Msa1340990 | AT5G15040.1 | 37.681 | 69 | 43 | 0 | 206 | 274 | 4 | 72 | 5.02e-11 | 58.9 |
| Msa1340990 | AT5G15040.3 | 41.791 | 67 | 39 | 0 | 99 | 165 | 6 | 72 | 1.19e-11 | 60.5 |
| Msa1340990 | AT5G15040.3 | 37.681 | 69 | 43 | 0 | 206 | 274 | 4 | 72 | 5.02e-11 | 58.9 |
| Msa1340990 | AT5G15040.2 | 41.791 | 67 | 39 | 0 | 99 | 165 | 6 | 72 | 1.19e-11 | 60.5 |
| Msa1340990 | AT5G15040.2 | 37.681 | 69 | 43 | 0 | 206 | 274 | 4 | 72 | 5.02e-11 | 58.9 |
Find 48 sgRNAs with CRISPR-Local
Find 128 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ACTGCTGTTGCAAACGAATT+TGG | 0.114157 | tig0004660:-1599 | None:intergenic |
| TGAAACTCCTACATGATTTC+AGG | 0.203193 | tig0004660:+1417 | Msa1340990:CDS |
| GTCCCAAAAGTAGATCTTTA+TGG | 0.235078 | tig0004660:-1745 | None:intergenic |
| AACTCTAAAATCAATTCTTT+TGG | 0.284021 | tig0004660:-2017 | None:intergenic |
| AACTCTCATATCAATTCTTT+TGG | 0.286653 | tig0004660:-1690 | None:intergenic |
| CTGCTGTTGCAAACGAATTT+GGG | 0.300087 | tig0004660:-1598 | None:intergenic |
| AACTCTTCTATCAATTCTTT+TGG | 0.305538 | tig0004660:-1441 | None:intergenic |
| AGACCATAAAGATCTACTTT+TGG | 0.325498 | tig0004660:+1742 | Msa1340990:CDS |
| TGAAGCAATGACGTTTATAA+AGG | 0.363280 | tig0004660:+1346 | Msa1340990:CDS |
| AGTAGAATTAATCCCTTGAT+TGG | 0.383598 | tig0004660:+1323 | Msa1340990:CDS |
| CGTCATTGCTTCACCAATCA+AGG | 0.390356 | tig0004660:-1336 | None:intergenic |
| AGTTGCATTTCCAGATAAGA+TGG | 0.401348 | tig0004660:+1625 | Msa1340990:CDS |
| AGTTGCATTTCAAGATAAGA+TGG | 0.422713 | tig0004660:+1952 | Msa1340990:CDS |
| ACTTGTGTTTCAAGATAAGA+AGG | 0.472355 | tig0004660:+1376 | Msa1340990:CDS |
| AGTTGTCAAAGATGGAGTTA+TGG | 0.472541 | tig0004660:+1460 | Msa1340990:CDS |
| TTCAACATCTTCTTGCCAGC+AGG | 0.472583 | tig0004660:+1518 | Msa1340990:CDS |
| CTTCTATTCTTCTTGCCACC+AGG | 0.488485 | tig0004660:+1554 | Msa1340990:CDS |
| GATAGAAGAGTTGTCAAAGA+TGG | 0.492886 | tig0004660:+1452 | Msa1340990:CDS |
| AATCAAGGGATTAATTCTAC+TGG | 0.502349 | tig0004660:-1321 | None:intergenic |
| GAAACTCCTACATGATTTCA+GGG | 0.508203 | tig0004660:+1418 | Msa1340990:CDS |
| ACACAAAATCTCACTTCCAT+TGG | 0.512949 | tig0004660:+2117 | Msa1340990:CDS |
| ACACAAAATCTCACTTCCAT+TGG | 0.514630 | tig0004660:+1790 | Msa1340990:CDS |
| GATGAACAACAAGGTGATGA+TGG | 0.529706 | tig0004660:+2145 | Msa1340990:CDS |
| GATGATGAACAACAAGGTGA+TGG | 0.541884 | tig0004660:+1866 | Msa1340990:CDS |
| AACGAATTTGGGAATAGAAG+TGG | 0.545086 | tig0004660:-1587 | None:intergenic |
| GATGAACAACAAGGTGGAGA+TGG | 0.545992 | tig0004660:+1818 | Msa1340990:CDS |
| TGAACTTCAAGTTGATGAAG+TGG | 0.554268 | tig0004660:+2231 | Msa1340990:CDS |
| CTCAACACTTGGTTGCCAGC+AGG | 0.555626 | tig0004660:+2094 | Msa1340990:CDS |
| GATGCTGAACAACAAGGTGA+TGG | 0.558029 | tig0004660:+2193 | Msa1340990:CDS |
| ACGAATTTGGGAATAGAAGT+GGG | 0.571692 | tig0004660:-1586 | None:intergenic |
| AGTGGGATTTCATCTCCTGC+TGG | 0.573929 | tig0004660:-1533 | None:intergenic |
| TGTTGTTCATCACCATCCAA+TGG | 0.575143 | tig0004660:-1806 | None:intergenic |
| TGTTGTTCATCACCATCCAA+TGG | 0.575143 | tig0004660:-2133 | None:intergenic |
| AGAAGTGGGAATTCATGTCC+TGG | 0.583648 | tig0004660:-1572 | None:intergenic |
| ACTCAACACTTGGTTGCCAG+TGG | 0.586298 | tig0004660:+1766 | Msa1340990:CDS |
| GTCATTGCTTCACCAATCAA+GGG | 0.590508 | tig0004660:-1335 | None:intergenic |
| AAAATCTCACTTCCATTGGA+TGG | 0.592611 | tig0004660:+1794 | Msa1340990:CDS |
| AAAATCTCACTTCCATTGGA+TGG | 0.592611 | tig0004660:+2121 | Msa1340990:CDS |
| GGTGGCAAGAAGAATAGAAG+TGG | 0.618772 | tig0004660:-1551 | None:intergenic |
| TGAACAACAAGGTGATGGAT+CGG | 0.624867 | tig0004660:+1871 | Msa1340990:CDS |
| CAGAAAGATGCTGAACAACA+AGG | 0.627330 | tig0004660:+2187 | Msa1340990:CDS |
| CTCAACACTTGGTTGCCAGT+GGG | 0.645795 | tig0004660:+1767 | Msa1340990:CDS |
| GTGGCAAGAAGAATAGAAGT+GGG | 0.680483 | tig0004660:-1550 | None:intergenic |
| CAGAAAGATGATGAACAACA+AGG | 0.695192 | tig0004660:+1860 | Msa1340990:CDS |
| AGTGGGAATTCATGTCCTGG+TGG | 0.708807 | tig0004660:-1569 | None:intergenic |
| GATGGTGATGAACAACAAGG+TGG | 0.767576 | tig0004660:+1812 | Msa1340990:CDS |
| TTGGATGGTGATGAACAACA+AGG | 0.791947 | tig0004660:+1809 | Msa1340990:CDS |
| TTGGATGGTGATGAACAACA+AGG | 0.791947 | tig0004660:+2136 | Msa1340990:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AACTCTAAAATCAATTCTTT+TGG | - | tig0004660:2020-2039 | None:intergenic | 20.0% |
| !!! | AGACCATAAAAATTTACTTT+TGG | + | tig0004660:2069-2088 | Msa1340990:CDS | 20.0% |
| !!! | GACCATAAAAATTTACTTTT+GGG | + | tig0004660:2070-2089 | Msa1340990:CDS | 20.0% |
| !!! | AACTCTAAAATCAATTCTTT+TGG | - | tig0004660:2020-2039 | None:intergenic | 20.0% |
| !!! | AGACCATAAAAATTTACTTT+TGG | + | tig0004660:2069-2088 | Msa1340990:CDS | 20.0% |
| !!! | GACCATAAAAATTTACTTTT+GGG | + | tig0004660:2070-2089 | Msa1340990:CDS | 20.0% |
| !! | AACTCTTCTATCAATTCTTT+TGG | - | tig0004660:1444-1463 | None:intergenic | 25.0% |
| !! | AACTCTCATATCAATTCTTT+TGG | - | tig0004660:1693-1712 | None:intergenic | 25.0% |
| !!! | GATTTTAGAGTTTTCAAAGA+AGG | + | tig0004660:2028-2047 | Msa1340990:CDS | 25.0% |
| !! | GTCCCAAAAGTAAATTTTTA+TGG | - | tig0004660:2075-2094 | None:intergenic | 25.0% |
| !! | AACTCTTCTATCAATTCTTT+TGG | - | tig0004660:1444-1463 | None:intergenic | 25.0% |
| !! | AACTCTCATATCAATTCTTT+TGG | - | tig0004660:1693-1712 | None:intergenic | 25.0% |
| !!! | GATTTTAGAGTTTTCAAAGA+AGG | + | tig0004660:2028-2047 | Msa1340990:CDS | 25.0% |
| !! | GTCCCAAAAGTAAATTTTTA+TGG | - | tig0004660:2075-2094 | None:intergenic | 25.0% |
| AATCAAGGGATTAATTCTAC+TGG | - | tig0004660:1324-1343 | None:intergenic | 30.0% | |
| AGTAGAATTAATCCCTTGAT+TGG | + | tig0004660:1323-1342 | Msa1340990:CDS | 30.0% | |
| TGAAGCAATGACGTTTATAA+AGG | + | tig0004660:1346-1365 | Msa1340990:CDS | 30.0% | |
| ACTTGTGTTTCAAGATAAGA+AGG | + | tig0004660:1376-1395 | Msa1340990:CDS | 30.0% | |
| ! | TCATACTTTTCCATCTTATC+TGG | - | tig0004660:1638-1657 | None:intergenic | 30.0% |
| ! | GATATGAGAGTTTTCAAAGA+AGG | + | tig0004660:1701-1720 | Msa1340990:CDS | 30.0% |
| ! | AGACCATAAAGATCTACTTT+TGG | + | tig0004660:1742-1761 | Msa1340990:CDS | 30.0% |
| !! | GACCATAAAGATCTACTTTT+GGG | + | tig0004660:1743-1762 | Msa1340990:CDS | 30.0% |
| AGTTGCATTTCAAGATAAGA+TGG | + | tig0004660:1952-1971 | Msa1340990:CDS | 30.0% | |
| ! | TAGAGTTTTCAAAGAAGGAA+TGG | + | tig0004660:2033-2052 | Msa1340990:CDS | 30.0% |
| AATCAAGGGATTAATTCTAC+TGG | - | tig0004660:1324-1343 | None:intergenic | 30.0% | |
| AGTAGAATTAATCCCTTGAT+TGG | + | tig0004660:1323-1342 | Msa1340990:CDS | 30.0% | |
| TGAAGCAATGACGTTTATAA+AGG | + | tig0004660:1346-1365 | Msa1340990:CDS | 30.0% | |
| ACTTGTGTTTCAAGATAAGA+AGG | + | tig0004660:1376-1395 | Msa1340990:CDS | 30.0% | |
| ! | TCATACTTTTCCATCTTATC+TGG | - | tig0004660:1638-1657 | None:intergenic | 30.0% |
| ! | GATATGAGAGTTTTCAAAGA+AGG | + | tig0004660:1701-1720 | Msa1340990:CDS | 30.0% |
| ! | AGACCATAAAGATCTACTTT+TGG | + | tig0004660:1742-1761 | Msa1340990:CDS | 30.0% |
| !! | GACCATAAAGATCTACTTTT+GGG | + | tig0004660:1743-1762 | Msa1340990:CDS | 30.0% |
| AGTTGCATTTCAAGATAAGA+TGG | + | tig0004660:1952-1971 | Msa1340990:CDS | 30.0% | |
| ! | TAGAGTTTTCAAAGAAGGAA+TGG | + | tig0004660:2033-2052 | Msa1340990:CDS | 30.0% |
| TGAAACTCCTACATGATTTC+AGG | + | tig0004660:1417-1436 | Msa1340990:CDS | 35.0% | |
| GAAACTCCTACATGATTTCA+GGG | + | tig0004660:1418-1437 | Msa1340990:CDS | 35.0% | |
| GATAGAAGAGTTGTCAAAGA+TGG | + | tig0004660:1452-1471 | Msa1340990:CDS | 35.0% | |
| AGTTGTCAAAGATGGAGTTA+TGG | + | tig0004660:1460-1479 | Msa1340990:CDS | 35.0% | |
| ACGAATTTGGGAATAGAAGT+GGG | - | tig0004660:1589-1608 | None:intergenic | 35.0% | |
| AACGAATTTGGGAATAGAAG+TGG | - | tig0004660:1590-1609 | None:intergenic | 35.0% | |
| AGTTGCATTTCCAGATAAGA+TGG | + | tig0004660:1625-1644 | Msa1340990:CDS | 35.0% | |
| ! | GAGAGTTTTCAAAGAAGGAA+TGG | + | tig0004660:1706-1725 | Msa1340990:CDS | 35.0% |
| ! | AGTTTTCAAAGAAGGAATGG+TGG | + | tig0004660:1709-1728 | Msa1340990:CDS | 35.0% |
| GTCCCAAAAGTAGATCTTTA+TGG | - | tig0004660:1748-1767 | None:intergenic | 35.0% | |
| ACACAAAATCTCACTTCCAT+TGG | + | tig0004660:1790-1809 | Msa1340990:CDS | 35.0% | |
| AAAATCTCACTTCCATTGGA+TGG | + | tig0004660:1794-1813 | Msa1340990:CDS | 35.0% | |
| CAGAAAGATGATGAACAACA+AGG | + | tig0004660:1860-1879 | Msa1340990:CDS | 35.0% | |
| ! | AGTTTTCAAAGAAGGAATGG+TGG | + | tig0004660:1709-1728 | Msa1340990:CDS | 35.0% |
| ACACAAAATCTCACTTCCAT+TGG | + | tig0004660:1790-1809 | Msa1340990:CDS | 35.0% | |
| AAAATCTCACTTCCATTGGA+TGG | + | tig0004660:1794-1813 | Msa1340990:CDS | 35.0% | |
| !! | TGAACTTCAAGTTGATGAAG+TGG | + | tig0004660:2231-2250 | Msa1340990:CDS | 35.0% |
| TGAAACTCCTACATGATTTC+AGG | + | tig0004660:1417-1436 | Msa1340990:CDS | 35.0% | |
| GAAACTCCTACATGATTTCA+GGG | + | tig0004660:1418-1437 | Msa1340990:CDS | 35.0% | |
| GATAGAAGAGTTGTCAAAGA+TGG | + | tig0004660:1452-1471 | Msa1340990:CDS | 35.0% | |
| AGTTGTCAAAGATGGAGTTA+TGG | + | tig0004660:1460-1479 | Msa1340990:CDS | 35.0% | |
| ACGAATTTGGGAATAGAAGT+GGG | - | tig0004660:1589-1608 | None:intergenic | 35.0% | |
| AACGAATTTGGGAATAGAAG+TGG | - | tig0004660:1590-1609 | None:intergenic | 35.0% | |
| AGTTGCATTTCCAGATAAGA+TGG | + | tig0004660:1625-1644 | Msa1340990:CDS | 35.0% | |
| ! | GAGAGTTTTCAAAGAAGGAA+TGG | + | tig0004660:1706-1725 | Msa1340990:CDS | 35.0% |
| ! | AGTTTTCAAAGAAGGAATGG+TGG | + | tig0004660:1709-1728 | Msa1340990:CDS | 35.0% |
| GTCCCAAAAGTAGATCTTTA+TGG | - | tig0004660:1748-1767 | None:intergenic | 35.0% | |
| ACACAAAATCTCACTTCCAT+TGG | + | tig0004660:1790-1809 | Msa1340990:CDS | 35.0% | |
| AAAATCTCACTTCCATTGGA+TGG | + | tig0004660:1794-1813 | Msa1340990:CDS | 35.0% | |
| CAGAAAGATGATGAACAACA+AGG | + | tig0004660:1860-1879 | Msa1340990:CDS | 35.0% | |
| ! | AGTTTTCAAAGAAGGAATGG+TGG | + | tig0004660:1709-1728 | Msa1340990:CDS | 35.0% |
| ACACAAAATCTCACTTCCAT+TGG | + | tig0004660:1790-1809 | Msa1340990:CDS | 35.0% | |
| AAAATCTCACTTCCATTGGA+TGG | + | tig0004660:1794-1813 | Msa1340990:CDS | 35.0% | |
| !! | TGAACTTCAAGTTGATGAAG+TGG | + | tig0004660:2231-2250 | Msa1340990:CDS | 35.0% |
| GTCATTGCTTCACCAATCAA+GGG | - | tig0004660:1338-1357 | None:intergenic | 40.0% | |
| ! | TTTTGGCCCTGAAATCATGT+AGG | - | tig0004660:1427-1446 | None:intergenic | 40.0% |
| GTGGCAAGAAGAATAGAAGT+GGG | - | tig0004660:1553-1572 | None:intergenic | 40.0% | |
| CTGCTGTTGCAAACGAATTT+GGG | - | tig0004660:1601-1620 | None:intergenic | 40.0% | |
| ACTGCTGTTGCAAACGAATT+TGG | - | tig0004660:1602-1621 | None:intergenic | 40.0% | |
| !!! | TACTTTTGGGACTCAACACT+TGG | + | tig0004660:1756-1775 | Msa1340990:CDS | 40.0% |
| TGTTGTTCATCACCATCCAA+TGG | - | tig0004660:1809-1828 | None:intergenic | 40.0% | |
| ! | TTGGATGGTGATGAACAACA+AGG | + | tig0004660:1809-1828 | Msa1340990:CDS | 40.0% |
| GATGATGAACAACAAGGTGA+TGG | + | tig0004660:1866-1885 | Msa1340990:CDS | 40.0% | |
| !! | TGAACAACAAGGTGATGGAT+CGG | + | tig0004660:1871-1890 | Msa1340990:CDS | 40.0% |
| !!! | TACTTTTGGGACTCAACACT+TGG | + | tig0004660:1756-1775 | Msa1340990:CDS | 40.0% |
| TGTTGTTCATCACCATCCAA+TGG | - | tig0004660:1809-1828 | None:intergenic | 40.0% | |
| ! | TTGGATGGTGATGAACAACA+AGG | + | tig0004660:1809-1828 | Msa1340990:CDS | 40.0% |
| GATGAACAACAAGGTGATGA+TGG | + | tig0004660:2145-2164 | Msa1340990:CDS | 40.0% | |
| CAGAAAGATGCTGAACAACA+AGG | + | tig0004660:2187-2206 | Msa1340990:CDS | 40.0% | |
| GTCATTGCTTCACCAATCAA+GGG | - | tig0004660:1338-1357 | None:intergenic | 40.0% | |
| ! | TTTTGGCCCTGAAATCATGT+AGG | - | tig0004660:1427-1446 | None:intergenic | 40.0% |
| GTGGCAAGAAGAATAGAAGT+GGG | - | tig0004660:1553-1572 | None:intergenic | 40.0% | |
| CTGCTGTTGCAAACGAATTT+GGG | - | tig0004660:1601-1620 | None:intergenic | 40.0% | |
| ACTGCTGTTGCAAACGAATT+TGG | - | tig0004660:1602-1621 | None:intergenic | 40.0% | |
| !!! | TACTTTTGGGACTCAACACT+TGG | + | tig0004660:1756-1775 | Msa1340990:CDS | 40.0% |
| TGTTGTTCATCACCATCCAA+TGG | - | tig0004660:1809-1828 | None:intergenic | 40.0% | |
| ! | TTGGATGGTGATGAACAACA+AGG | + | tig0004660:1809-1828 | Msa1340990:CDS | 40.0% |
| GATGATGAACAACAAGGTGA+TGG | + | tig0004660:1866-1885 | Msa1340990:CDS | 40.0% | |
| !! | TGAACAACAAGGTGATGGAT+CGG | + | tig0004660:1871-1890 | Msa1340990:CDS | 40.0% |
| !!! | TACTTTTGGGACTCAACACT+TGG | + | tig0004660:1756-1775 | Msa1340990:CDS | 40.0% |
| TGTTGTTCATCACCATCCAA+TGG | - | tig0004660:1809-1828 | None:intergenic | 40.0% | |
| ! | TTGGATGGTGATGAACAACA+AGG | + | tig0004660:1809-1828 | Msa1340990:CDS | 40.0% |
| GATGAACAACAAGGTGATGA+TGG | + | tig0004660:2145-2164 | Msa1340990:CDS | 40.0% | |
| CAGAAAGATGCTGAACAACA+AGG | + | tig0004660:2187-2206 | Msa1340990:CDS | 40.0% | |
| CGTCATTGCTTCACCAATCA+AGG | - | tig0004660:1339-1358 | None:intergenic | 45.0% | |
| TTCAACATCTTCTTGCCAGC+AGG | + | tig0004660:1518-1537 | Msa1340990:CDS | 45.0% | |
| GGTGGCAAGAAGAATAGAAG+TGG | - | tig0004660:1554-1573 | None:intergenic | 45.0% | |
| CTTCTATTCTTCTTGCCACC+AGG | + | tig0004660:1554-1573 | Msa1340990:CDS | 45.0% | |
| AGAAGTGGGAATTCATGTCC+TGG | - | tig0004660:1575-1594 | None:intergenic | 45.0% | |
| ! | AGTGAGATTTTGTGTCCCAC+TGG | - | tig0004660:1785-1804 | None:intergenic | 45.0% |
| ! | GATGGTGATGAACAACAAGG+TGG | + | tig0004660:1812-1831 | Msa1340990:CDS | 45.0% |
| GATGAACAACAAGGTGGAGA+TGG | + | tig0004660:1818-1837 | Msa1340990:CDS | 45.0% | |
| ! | AGTGAGATTTTGTGTCCTGC+TGG | - | tig0004660:2112-2131 | None:intergenic | 45.0% |
| GATGCTGAACAACAAGGTGA+TGG | + | tig0004660:2193-2212 | Msa1340990:CDS | 45.0% | |
| CGTCATTGCTTCACCAATCA+AGG | - | tig0004660:1339-1358 | None:intergenic | 45.0% | |
| TTCAACATCTTCTTGCCAGC+AGG | + | tig0004660:1518-1537 | Msa1340990:CDS | 45.0% | |
| GGTGGCAAGAAGAATAGAAG+TGG | - | tig0004660:1554-1573 | None:intergenic | 45.0% | |
| CTTCTATTCTTCTTGCCACC+AGG | + | tig0004660:1554-1573 | Msa1340990:CDS | 45.0% | |
| AGAAGTGGGAATTCATGTCC+TGG | - | tig0004660:1575-1594 | None:intergenic | 45.0% | |
| ! | AGTGAGATTTTGTGTCCCAC+TGG | - | tig0004660:1785-1804 | None:intergenic | 45.0% |
| ! | GATGGTGATGAACAACAAGG+TGG | + | tig0004660:1812-1831 | Msa1340990:CDS | 45.0% |
| GATGAACAACAAGGTGGAGA+TGG | + | tig0004660:1818-1837 | Msa1340990:CDS | 45.0% | |
| ! | AGTGAGATTTTGTGTCCTGC+TGG | - | tig0004660:2112-2131 | None:intergenic | 45.0% |
| GATGCTGAACAACAAGGTGA+TGG | + | tig0004660:2193-2212 | Msa1340990:CDS | 45.0% | |
| AGTGGGATTTCATCTCCTGC+TGG | - | tig0004660:1536-1555 | None:intergenic | 50.0% | |
| AGTGGGAATTCATGTCCTGG+TGG | - | tig0004660:1572-1591 | None:intergenic | 50.0% | |
| ACTCAACACTTGGTTGCCAG+TGG | + | tig0004660:1766-1785 | Msa1340990:CDS | 50.0% | |
| CTCAACACTTGGTTGCCAGT+GGG | + | tig0004660:1767-1786 | Msa1340990:CDS | 50.0% | |
| AGTGGGATTTCATCTCCTGC+TGG | - | tig0004660:1536-1555 | None:intergenic | 50.0% | |
| AGTGGGAATTCATGTCCTGG+TGG | - | tig0004660:1572-1591 | None:intergenic | 50.0% | |
| ACTCAACACTTGGTTGCCAG+TGG | + | tig0004660:1766-1785 | Msa1340990:CDS | 50.0% | |
| CTCAACACTTGGTTGCCAGT+GGG | + | tig0004660:1767-1786 | Msa1340990:CDS | 50.0% | |
| CTCAACACTTGGTTGCCAGC+AGG | + | tig0004660:2094-2113 | Msa1340990:CDS | 55.0% | |
| CTCAACACTTGGTTGCCAGC+AGG | + | tig0004660:2094-2113 | Msa1340990:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| tig0004660 | gene | 1311 | 2288 | 1311 | ID=Msa1340990;Name=Msa1340990 |
| tig0004660 | mRNA | 1311 | 2288 | 1311 | ID=Msa1340990-mRNA-1;Parent=Msa1340990;Name=Msa1340990-mRNA-1;_AED=0.01;_eAED=0.01;_QI=0|-1|0|1|-1|1|1|0|325 |
| tig0004660 | exon | 1311 | 2288 | 1311 | ID=Msa1340990-mRNA-1:exon:2947;Parent=Msa1340990-mRNA-1 |
| tig0004660 | CDS | 1311 | 2288 | 1311 | ID=Msa1340990-mRNA-1:cds;Parent=Msa1340990-mRNA-1 |
| Gene Sequence |
| Protein sequence |
