AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1341240

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1341240 MtrunA17_Chr4g0067981 98.611 504 6 1 1 504 178 680 0.0 1009
Msa1341240 MtrunA17_Chr2g0294911 55.929 506 139 12 2 504 177 601 5.75e-162 472
Msa1341240 MtrunA17_Chr1g0161431 51.724 174 74 5 1 168 158 327 2.80e-41 156
Msa1341240 MtrunA17_Chr3g0137101 74.026 77 20 0 27 103 187 263 8.37e-31 126
Msa1341240 MtrunA17_Chr3g0123131 60.870 92 34 1 17 106 176 267 1.48e-27 116
Msa1341240 MtrunA17_Chr8g0376881 60.204 98 35 2 1 96 172 267 6.93e-27 114
Msa1341240 MtrunA17_Chr2g0304841 41.848 184 88 7 34 210 209 380 7.01e-27 114
Msa1341240 MtrunA17_Chr1g0149841 55.340 103 38 1 11 105 156 258 3.66e-26 111
Msa1341240 MtrunA17_Chr4g0031961 54.082 98 37 2 11 100 96 193 8.93e-25 104
Msa1341240 MtrunA17_Chr4g0074411 42.675 157 73 5 34 179 197 347 3.09e-24 106
Msa1341240 MtrunA17_Chr7g0255711 65.672 67 23 0 33 99 86 152 1.38e-23 100
Msa1341240 MtrunA17_Chr1g0149831 65.753 73 22 1 33 105 123 192 1.69e-23 99.0
Msa1341240 MtrunA17_Chr4g0074401 41.212 165 79 6 34 187 199 356 1.57e-22 101
Msa1341240 MtrunA17_Chr6g0473731 46.939 98 46 1 15 106 72 169 1.07e-20 94.7
Msa1341240 MtrunA17_Chr4g0074421 63.077 65 24 0 28 92 123 187 2.20e-20 89.4
Msa1341240 MtrunA17_Chr7g0275891 48.000 100 35 2 37 123 190 285 4.53e-20 91.7
Msa1341240 MtrunA17_Chr4g0009851 57.971 69 29 0 26 94 157 225 5.69e-20 90.9
Msa1341240 MtrunA17_Chr5g0400241 57.971 69 29 0 28 96 301 369 1.88e-19 91.3
Msa1341240 MtrunA17_Chr3g0134461 60.000 75 22 2 14 87 125 192 2.53e-19 86.3
Msa1341240 MtrunA17_Chr7g0275401 40.580 138 74 2 2 131 148 285 5.40e-19 87.4
Msa1341240 MtrunA17_Chr1g0149851 52.381 84 25 1 33 101 186 269 9.73e-19 89.4
Msa1341240 MtrunA17_Chr4g0009981 51.852 81 34 2 15 94 96 172 2.74e-18 84.7
Msa1341240 MtrunA17_Chr4g0054981 50.000 78 37 1 37 114 309 384 2.96e-18 87.8
Msa1341240 MtrunA17_Chr4g0009901 51.852 81 36 1 15 95 148 225 4.04e-18 84.7
Msa1341240 MtrunA17_Chr3g0134481 62.264 53 20 0 37 89 131 183 7.63e-17 79.0
Msa1341240 MtrunA17_Chr4g0040481 58.333 60 25 0 37 96 157 216 1.37e-16 82.0
Msa1341240 MtrunA17_Chr2g0324891 58.333 60 25 0 37 96 160 219 2.32e-16 81.3
Msa1341240 MtrunA17_Chr1g0198601 50.000 82 40 1 26 106 199 280 3.06e-16 80.5
Msa1341240 MtrunA17_Chr6g0471041 58.621 58 24 0 37 94 171 228 3.89e-16 80.5
Msa1341240 MtrunA17_Chr6g0469321 67.308 52 17 0 54 105 70 121 5.80e-16 75.9
Msa1341240 MtrunA17_Chr7g0223971 38.095 105 32 1 26 97 77 181 9.84e-16 79.7
Msa1341240 MtrunA17_Chr4g0009991 53.521 71 28 2 18 87 50 116 1.00e-14 70.9
Msa1341240 MtrunA17_Chr5g0417521 57.627 59 24 1 34 91 11 69 1.05e-14 75.5
Msa1341240 MtrunA17_Chr7g0274471 46.739 92 40 2 38 128 218 301 1.44e-14 75.9
Msa1341240 MtrunA17_Chr5g0422621 39.496 119 58 5 25 131 186 302 2.28e-14 74.7
Msa1341240 MtrunA17_Chr6g0461641 55.932 59 25 1 34 91 38 96 5.60e-14 73.6
Msa1341240 MtrunA17_Chr2g0321461 55.932 59 25 1 34 91 41 99 9.97e-14 72.8
Msa1341240 MtrunA17_Chr4g0042451 54.237 59 26 1 34 91 13 71 3.04e-13 70.9
Msa1341240 MtrunA17_Chr2g0310011 57.627 59 24 1 34 91 31 89 4.81e-13 70.5
Msa1341240 MtrunA17_Chr7g0261911 44.792 96 44 3 34 128 258 345 6.39e-13 70.9
Msa1341240 MtrunA17_Chr8g0356501 50.649 77 37 1 16 91 15 91 6.64e-13 70.1
Msa1341240 MtrunA17_Chr1g0190301 43.478 92 43 2 38 128 175 258 9.12e-13 70.1
Msa1341240 MtrunA17_Chr4g0045361 53.731 67 30 1 35 100 271 337 9.96e-13 70.1
Msa1341240 MtrunA17_Chr5g0402631 55.738 61 26 1 37 96 213 273 1.10e-12 69.3
Msa1341240 MtrunA17_Chr4g0062921 52.941 68 31 1 35 101 232 299 1.15e-12 69.7
Msa1341240 MtrunA17_Chr1g0197171 60.000 55 21 1 38 91 44 98 1.87e-12 68.2
Msa1341240 MtrunA17_Chr5g0409731 56.364 55 23 1 38 91 25 79 6.70e-12 66.2
Msa1341240 MtrunA17_Chr7g0254981 56.364 55 23 1 38 91 50 104 6.97e-12 66.2
Msa1341240 MtrunA17_Chr8g0391911 52.381 63 24 1 41 97 190 252 1.62e-11 64.7
Msa1341240 MtrunA17_Chr2g0290991 58.182 55 22 1 38 91 39 93 2.15e-11 65.1
Msa1341240 MtrunA17_Chr2g0291021 58.182 55 22 1 38 91 49 103 3.57e-11 64.7
Msa1341240 MtrunA17_Chr1g0175081 50.943 53 25 1 40 91 24 76 6.34e-11 59.3
Msa1341240 MtrunA17_Chr1g0162931 41.111 90 48 2 37 121 17 106 8.80e-11 62.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1341240 AT3G16857.3 47.672 537 201 26 4 504 128 620 1.33e-118 362
Msa1341240 AT3G16857.2 47.269 531 212 24 4 504 198 690 6.10e-118 362
Msa1341240 AT3G16857.1 46.457 508 205 23 4 481 198 668 3.46e-107 334
Msa1341240 AT2G01760.3 60.748 107 31 1 37 143 134 229 9.47e-33 127
Msa1341240 AT2G01760.1 60.748 107 31 1 37 143 199 294 4.75e-32 127
Msa1341240 AT2G01760.2 60.748 107 31 1 37 143 227 322 1.06e-31 126
Msa1341240 AT5G58080.1 54.867 113 48 2 29 140 185 295 2.36e-29 122
Msa1341240 AT2G25180.1 37.945 253 122 10 9 237 161 402 5.18e-28 118
Msa1341240 AT4G31920.1 46.667 120 55 2 12 124 150 267 9.62e-25 108
Msa1341240 AT3G46640.2 60.759 79 28 1 25 100 128 206 1.20e-24 104
Msa1341240 AT3G46640.1 60.759 79 28 1 25 100 128 206 1.20e-24 104
Msa1341240 AT3G46640.3 60.759 79 28 1 25 100 128 206 1.76e-24 104
Msa1341240 AT5G59570.2 59.036 83 31 2 25 104 125 207 6.33e-24 102
Msa1341240 AT5G59570.1 59.036 83 31 2 25 104 125 207 6.33e-24 102
Msa1341240 AT5G05090.1 62.667 75 26 1 24 96 65 139 2.92e-23 99.8
Msa1341240 AT2G40970.1 66.667 66 22 0 31 96 98 163 3.60e-23 99.0
Msa1341240 AT3G10760.1 69.355 62 19 0 35 96 102 163 1.17e-22 99.4
Msa1341240 AT1G67710.3 63.529 85 25 2 18 96 94 178 8.36e-21 95.5
Msa1341240 AT1G67710.2 79.310 58 11 1 40 96 130 187 1.04e-20 95.1
Msa1341240 AT1G67710.1 79.310 58 11 1 40 96 195 252 1.59e-20 95.1
Msa1341240 AT5G07210.1 51.765 85 37 1 17 97 205 289 3.88e-20 94.4
Msa1341240 AT5G07210.2 51.765 85 37 1 17 97 197 281 4.25e-20 94.0
Msa1341240 AT5G44190.2 62.121 66 25 0 31 96 140 205 7.81e-20 91.3
Msa1341240 AT5G44190.1 62.121 66 25 0 31 96 140 205 8.55e-20 91.7
Msa1341240 AT2G27070.1 51.190 84 37 1 17 96 200 283 4.47e-19 90.9
Msa1341240 AT5G49240.1 50.617 81 40 0 22 102 207 287 2.60e-17 82.8
Msa1341240 AT2G20570.1 51.087 92 31 2 5 96 134 211 3.76e-17 84.0
Msa1341240 AT2G20570.2 48.936 94 46 1 5 96 134 227 1.49e-16 82.4
Msa1341240 AT4G18020.8 49.315 73 36 1 40 112 298 369 6.15e-15 77.8
Msa1341240 AT4G18020.11 49.315 73 36 1 40 112 298 369 6.15e-15 77.8
Msa1341240 AT4G18020.4 49.315 73 36 1 40 112 298 369 6.15e-15 77.8
Msa1341240 AT4G18020.5 49.315 73 36 1 40 112 298 369 6.15e-15 77.8
Msa1341240 AT4G18020.10 49.315 73 36 1 40 112 298 369 6.15e-15 77.8
Msa1341240 AT4G18020.7 49.315 73 36 1 40 112 298 369 6.15e-15 77.8
Msa1341240 AT4G18020.3 48.000 75 38 1 38 112 296 369 6.92e-15 77.8
Msa1341240 AT4G18020.2 48.000 75 38 1 38 112 296 369 6.92e-15 77.8
Msa1341240 AT4G18020.1 48.000 75 38 1 38 112 296 369 6.92e-15 77.8
Msa1341240 AT4G18020.6 48.000 75 38 1 38 112 296 369 6.92e-15 77.8
Msa1341240 AT4G18020.9 48.000 75 38 1 38 112 296 369 6.92e-15 77.8
Msa1341240 AT3G04030.1 52.000 75 31 3 34 107 41 111 3.54e-14 74.7
Msa1341240 AT3G04030.3 52.000 75 31 3 34 107 41 111 3.80e-14 74.7
Msa1341240 AT3G62670.1 53.623 69 27 2 27 90 200 268 3.91e-14 74.7
Msa1341240 AT5G18240.2 57.627 59 24 1 34 91 41 99 1.12e-13 73.2
Msa1341240 AT5G18240.3 57.627 59 24 1 34 91 41 99 1.12e-13 73.2
Msa1341240 AT5G18240.5 57.627 59 24 1 34 91 41 99 1.23e-13 73.2
Msa1341240 AT5G18240.1 57.627 59 24 1 34 91 41 99 1.29e-13 73.2
Msa1341240 AT5G18240.4 57.627 59 24 1 34 91 41 99 1.29e-13 73.2
Msa1341240 AT5G29000.2 40.000 110 56 3 22 128 215 317 1.37e-13 73.2
Msa1341240 AT5G29000.1 40.000 110 56 3 22 128 172 274 1.39e-13 72.8
Msa1341240 AT5G29000.3 40.000 110 56 3 22 128 172 274 1.39e-13 72.8
Msa1341240 AT3G04030.2 48.649 74 34 2 34 107 41 110 6.36e-13 70.9
Msa1341240 AT1G79430.2 57.627 59 24 1 34 91 30 88 7.05e-13 70.5
Msa1341240 AT3G04450.4 42.424 99 47 3 33 128 193 284 7.40e-13 70.9
Msa1341240 AT3G04450.2 42.424 99 47 3 33 128 193 284 7.40e-13 70.9
Msa1341240 AT3G04450.1 42.424 99 47 3 33 128 233 324 7.54e-13 70.9
Msa1341240 AT1G69580.2 52.542 59 27 1 34 91 30 88 1.74e-12 68.9
Msa1341240 AT1G69580.1 52.542 59 27 1 34 91 30 88 1.74e-12 68.9
Msa1341240 AT3G24120.2 58.182 55 22 1 38 91 41 95 2.01e-12 68.6
Msa1341240 AT3G24120.1 58.182 55 22 1 38 91 41 95 2.07e-12 68.2
Msa1341240 AT5G45580.1 56.364 55 23 1 38 91 7 61 2.27e-12 67.8
Msa1341240 AT5G45580.2 56.364 55 23 1 38 91 23 77 2.62e-12 67.8
Msa1341240 AT3G12730.1 52.174 69 26 2 30 91 9 77 2.66e-12 67.0
Msa1341240 AT1G25550.1 48.611 72 36 1 25 95 196 267 3.01e-12 68.6
Msa1341240 AT2G03500.1 54.839 62 27 1 37 97 232 293 3.27e-12 68.9
Msa1341240 AT5G29000.4 48.571 70 35 1 22 90 215 284 3.60e-12 67.8
Msa1341240 AT4G13640.2 58.182 55 22 1 38 91 37 91 5.58e-12 67.0
Msa1341240 AT4G13640.1 58.182 55 22 1 38 91 37 91 6.05e-12 67.0
Msa1341240 AT3G04450.3 54.237 59 26 1 33 90 233 291 7.68e-12 67.0
Msa1341240 AT2G01060.1 55.738 61 26 1 32 91 9 69 1.55e-11 65.5
Msa1341240 AT1G68670.1 46.914 81 40 2 23 100 198 278 3.81e-11 65.1

Find 160 sgRNAs with CRISPR-Local

Find 229 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCAAATATTCTTCAAACTTC+TGG 0.147805 tig0004802:-30482 Msa1341240:three_prime_UTR
TGGAATTTCTGACGGTCTTA+TGG 0.190497 tig0004802:-31418 Msa1341240:CDS
TCATCCTGCCCGAAATTATC+AGG 0.256695 tig0004802:+30838 None:intergenic
AACCTCTTCCCTGATAATTT+CGG 0.273856 tig0004802:-30847 Msa1341240:CDS
TATCAGGGAAGAGGTTAGTA+TGG 0.283763 tig0004802:+30854 None:intergenic
ACCTCTTCCCTGATAATTTC+GGG 0.288070 tig0004802:-30846 Msa1341240:CDS
TTCTTTCTGATTGCAGTTAC+CGG 0.308965 tig0004802:-30600 Msa1341240:three_prime_UTR
TTGTGCCATCTGCATCAATA+AGG 0.312582 tig0004802:+31530 None:intergenic
CATTTCAGTGCTTTGATTCA+TGG 0.319626 tig0004802:+31317 None:intergenic
ATTTCAAGCCGAAGTTAATT+CGG 0.322921 tig0004802:-31232 Msa1341240:CDS
CTTCTGCTAATGAAGGAAAT+TGG 0.323941 tig0004802:-32979 Msa1341240:CDS
GGAAAATCTCCTTTCTCTCT+TGG 0.333648 tig0004802:-30988 Msa1341240:CDS
TTCCGGATGTTTATGTTCTT+CGG 0.335747 tig0004802:-30093 Msa1341240:three_prime_UTR
CTTCTCCAAATCTCAACCTT+GGG 0.339068 tig0004802:+31718 None:intergenic
TGAAGAGGTATGCTTGGGTT+AGG 0.342605 tig0004802:-30302 Msa1341240:three_prime_UTR
TGCAAATTGGCGAGTTGCTT+TGG 0.342865 tig0004802:+31621 None:intergenic
CTGGAATTGATGAATGTTCC+TGG 0.347527 tig0004802:-32149 Msa1341240:CDS
GGAATTTCTGACGGTCTTAT+GGG 0.349221 tig0004802:-31417 Msa1341240:CDS
GAACAAGGTAATCTCCAAAA+TGG 0.349947 tig0004802:-30955 Msa1341240:CDS
CTAGACTTTGCGCTGGGAGC+TGG 0.350433 tig0004802:+31835 None:intergenic
GAAAACCCAAGGTTGAGATT+TGG 0.350909 tig0004802:-31723 Msa1341240:CDS
AGCAGAGATGCTTCTTTGCT+TGG 0.351628 tig0004802:-31009 Msa1341240:CDS
AAATGTTGCCAGCCACTTGC+AGG 0.358647 tig0004802:-32114 Msa1341240:intron
GAAGTTAATTCGGGAATTAA+AGG 0.362242 tig0004802:-31222 Msa1341240:CDS
TAAAGAAGCCTCGGGTTGTT+TGG 0.371579 tig0004802:-32892 Msa1341240:CDS
CCAGCAGAAATCCCGGGATT+GGG 0.372360 tig0004802:-31157 Msa1341240:CDS
CAAATATTCTTCAAACTTCT+GGG 0.373124 tig0004802:-30481 Msa1341240:three_prime_UTR
CTTTGTACATGAACATGGTT+TGG 0.376891 tig0004802:-30033 Msa1341240:three_prime_UTR
TGAAAATTCAACAAGGGTTA+AGG 0.379057 tig0004802:-30902 Msa1341240:CDS
CTGGGAGTAGTGTACTATAA+TGG 0.381832 tig0004802:-30463 Msa1341240:three_prime_UTR
CCAAGATTACCGAACCTCTT+GGG 0.383663 tig0004802:-31459 Msa1341240:CDS
AATGTTGACTGCCACCATTT+TGG 0.384208 tig0004802:+30941 None:intergenic
CATGAGTGCACTTCTGAAAC+AGG 0.384874 tig0004802:-30812 Msa1341240:intron
TTTCAAGCCGAAGTTAATTC+GGG 0.385444 tig0004802:-31231 Msa1341240:CDS
AACGAAGAGCTCTGTGGAAC+AGG 0.393856 tig0004802:+31351 None:intergenic
TTTCTCTCTTGGAGAAGGCT+TGG 0.399650 tig0004802:-30977 Msa1341240:CDS
CAGCAGCCACAAACTGTTGA+TGG 0.399900 tig0004802:+32840 None:intergenic
TCTGTTAGTAATTTCCCTCT+TGG 0.407707 tig0004802:-31294 Msa1341240:CDS
CGAGCTCCATCAACAGTTTG+TGG 0.408027 tig0004802:-32864 Msa1341240:CDS
CCTTCTCCAAATCTCAACCT+TGG 0.410833 tig0004802:+31717 None:intergenic
CCCAAGAGGTTCGGTAATCT+TGG 0.413044 tig0004802:+31459 None:intergenic
TTCGGCTTGAAATGAGCCTT+TGG 0.414980 tig0004802:+31242 None:intergenic
GGCTGAGAAATGAATTATTC+TGG 0.423210 tig0004802:+31928 None:intergenic
CCCAATCCCGGGATTTCTGC+TGG 0.423497 tig0004802:+31157 None:intergenic
TTCCCTCTTGGAAGCACTCC+AGG 0.424109 tig0004802:-31282 Msa1341240:CDS
AGCCCACAAGAGGCATCATT+TGG 0.424795 tig0004802:-31909 Msa1341240:CDS
TGCTGCAGACTGGTGCAAAT+TGG 0.428667 tig0004802:+31608 None:intergenic
TGCTTTGGCTCCATGTTAGT+AGG 0.429194 tig0004802:+31636 None:intergenic
ACCAGCAGAAATCCCGGGAT+TGG 0.435479 tig0004802:-31158 Msa1341240:CDS
AGTCCCCGCTTCTGCTACAC+AGG 0.436130 tig0004802:-31559 Msa1341240:CDS
GTTCTTGAAGAGGTATGCTT+GGG 0.439729 tig0004802:-30307 Msa1341240:three_prime_UTR
GAAGAGGTATGCTTGGGTTA+GGG 0.445920 tig0004802:-30301 Msa1341240:three_prime_UTR
ACTCTTCAAGCAGCAGGACT+TGG 0.447810 tig0004802:-31810 Msa1341240:CDS
ATTTCAGTGCTTTGATTCAT+GGG 0.452203 tig0004802:+31318 None:intergenic
TTAGTATGGGAGCTCGCGTC+AGG 0.455250 tig0004802:+30868 None:intergenic
CTTTGATCCATGAGAGGCAT+TGG 0.455626 tig0004802:+31762 None:intergenic
TGTACTATAATGGTTTAGTC+TGG 0.456530 tig0004802:-30453 Msa1341240:three_prime_UTR
TGACTTTGACGGGTATTCTC+TGG 0.456582 tig0004802:-30656 Msa1341240:CDS
TCCAAGATTACCGAACCTCT+TGG 0.457743 tig0004802:-31460 Msa1341240:CDS
AGAATGAGTTTGACTTTGAC+GGG 0.458157 tig0004802:-30666 Msa1341240:CDS
TGTTCTTGAAGAGGTATGCT+TGG 0.458201 tig0004802:-30308 Msa1341240:three_prime_UTR
CTTCAAACTCTTGCAGCAGC+TGG 0.460273 tig0004802:-31858 Msa1341240:CDS
GAAGAACATAAAATTAAAAG+AGG 0.460919 tig0004802:+30184 None:intergenic
TCGGGAATTAAAGGATCAGC+TGG 0.463527 tig0004802:-31213 Msa1341240:CDS
AGGCTTACCAATGCCTCTCA+TGG 0.463932 tig0004802:-31769 Msa1341240:CDS
TATTCGTCTTCTGCTAATGA+AGG 0.466948 tig0004802:-32986 None:intergenic
AACTGAACCAGCAGAAATCC+CGG 0.470254 tig0004802:-31164 Msa1341240:CDS
TAGCATTGGACCTCTAGGTT+GGG 0.471457 tig0004802:+31506 None:intergenic
GGTAGGTCAACTGTGAAATC+AGG 0.471735 tig0004802:-31789 Msa1341240:CDS
TCACTTAGCATTGGACCTCT+AGG 0.481945 tig0004802:+31501 None:intergenic
TGCCAAATGATGCCTCTTGT+GGG 0.486957 tig0004802:+31907 None:intergenic
TTTCCCTGTGTAGCAGAAGC+GGG 0.487937 tig0004802:+31555 None:intergenic
AAACCAACAAATCTTCTTCA+CGG 0.488919 tig0004802:-31663 Msa1341240:CDS
ACTCCGTGAAGAAGATTTGT+TGG 0.489018 tig0004802:+31660 None:intergenic
CAGCAGAAATCCCGGGATTG+GGG 0.489487 tig0004802:-31156 Msa1341240:CDS
TCTCCTTTCTCTCTTGGAGA+AGG 0.491761 tig0004802:-30982 Msa1341240:CDS
TTTAATTCCCGAATTAACTT+CGG 0.493406 tig0004802:+31224 None:intergenic
CAAGGGATTGTGCTGCAGAC+TGG 0.501077 tig0004802:+31598 None:intergenic
TTCTTGCAGCAGGAAGGCAT+TGG 0.502837 tig0004802:-30697 Msa1341240:intron
GGGCAGTCTACTGTGTCAAA+TGG 0.502999 tig0004802:-31438 Msa1341240:CDS
AAGCCTTCTCCAAGAGAGAA+AGG 0.504157 tig0004802:+30979 None:intergenic
TGTGCCATCTGCATCAATAA+GGG 0.506429 tig0004802:+31531 None:intergenic
GACCGAAGAACATAAACATC+CGG 0.506870 tig0004802:+30091 None:intergenic
AGGAATCATATGTTAAACCT+TGG 0.509543 tig0004802:+31124 None:intergenic
GAGAATGAGTTTGACTTTGA+CGG 0.512088 tig0004802:-30667 Msa1341240:CDS
CCAATGCTAAGTGAAAATAC+TGG 0.512986 tig0004802:-31492 Msa1341240:CDS
GTATGATACCTGCAAGTGGC+TGG 0.513699 tig0004802:+32106 None:intergenic
GTAGACTGCCCCAAGAGGTT+CGG 0.516395 tig0004802:+31450 None:intergenic
AGAAGGGATGATGAAGAGGA+AGG 0.517568 tig0004802:-32941 Msa1341240:CDS
ACATCGATGTTGCCATGTAA+AGG 0.519974 tig0004802:+31087 None:intergenic
AGAAGCATCTCTGCTATGTC+CGG 0.523262 tig0004802:+31017 None:intergenic
AAAGAGAAGGGATGATGAAG+AGG 0.527771 tig0004802:-32945 Msa1341240:CDS
GTGCCAAATGATGCCTCTTG+TGG 0.529194 tig0004802:+31906 None:intergenic
CTTCCCTGATAATTTCGGGC+AGG 0.529625 tig0004802:-30842 Msa1341240:CDS
CGTCAACACTAAAGAAGCCT+CGG 0.531313 tig0004802:-32901 Msa1341240:CDS
CTCGCATATTCATGTTGCCA+AGG 0.540431 tig0004802:+31580 None:intergenic
TGGAATTGATGAATGTTCCT+GGG 0.540747 tig0004802:-32148 Msa1341240:CDS
CTAGCCACTCTTCAAGCAGC+AGG 0.540998 tig0004802:-31816 Msa1341240:CDS
TCAACAGTTTGTGGCTGCTG+TGG 0.541281 tig0004802:-32855 Msa1341240:CDS
TTAGCATTGGACCTCTAGGT+TGG 0.542224 tig0004802:+31505 None:intergenic
TTGTGTTTCTTGCAGCAGGA+AGG 0.544167 tig0004802:-30703 Msa1341240:intron
CAAGGTTGAGATTTGGAGAA+GGG 0.545110 tig0004802:-31716 Msa1341240:CDS
CAAGATTACCGAACCTCTTG+GGG 0.546140 tig0004802:-31458 Msa1341240:CDS
GGCACGATTTCGTCAATGAA+CGG 0.547334 tig0004802:-31888 Msa1341240:CDS
TTCATTTCTCAGCCCACAAG+AGG 0.554489 tig0004802:-31919 Msa1341240:CDS
GAGCAGAAGAAAAGCCCTGA+TGG 0.559461 tig0004802:+31046 None:intergenic
GCCCGAAATTATCAGGGAAG+AGG 0.559846 tig0004802:+30845 None:intergenic
CAGCAGGAAGGCATTGGACA+AGG 0.560036 tig0004802:-30691 Msa1341240:CDS
CAATGCCTCTCATGGATCAA+AGG 0.561353 tig0004802:-31761 Msa1341240:CDS
CTTGGAGAAGGCTTGGAACA+AGG 0.563016 tig0004802:-30970 Msa1341240:CDS
TCGCATGCAAATCCTTTACA+TGG 0.566200 tig0004802:-31099 Msa1341240:CDS
CAGCTGCTGCAAGAGTTTGA+AGG 0.567437 tig0004802:+31859 None:intergenic
GGAGAAACTCGAGAAAGAGA+AGG 0.568448 tig0004802:-32958 Msa1341240:CDS
AGGGCAGAATTGCAGAAGCA+AGG 0.569014 tig0004802:-30282 Msa1341240:three_prime_UTR
GAATTCTTTGTACATGAACA+TGG 0.574074 tig0004802:-30038 Msa1341240:three_prime_UTR
TAACTGCAATCAGAAAGAAC+TGG 0.577103 tig0004802:+30604 None:intergenic
AAATCCCTTATTGATGCAGA+TGG 0.581980 tig0004802:-31535 Msa1341240:CDS
TCACGGAGTTCCTACTAACA+TGG 0.585473 tig0004802:-31646 Msa1341240:CDS
AATATCGATTGTATCTTCGA+AGG 0.588508 tig0004802:-31971 Msa1341240:CDS
GTGGCTGCTGTGGATCAACT+AGG 0.590323 tig0004802:-32845 Msa1341240:CDS
CAGTCTGCAGCACAATCCCT+TGG 0.591458 tig0004802:-31597 Msa1341240:CDS
GAGAAACTCGAGAAAGAGAA+GGG 0.591527 tig0004802:-32957 Msa1341240:CDS
ATTTCCCTGTGTAGCAGAAG+CGG 0.596362 tig0004802:+31554 None:intergenic
ATCAGGGAAGAGGTTAGTAT+GGG 0.598648 tig0004802:+30855 None:intergenic
CCAAGGTTGAGATTTGGAGA+AGG 0.603069 tig0004802:-31717 Msa1341240:CDS
AGAGTGGCTAGACTTTGCGC+TGG 0.604456 tig0004802:+31828 None:intergenic
TTATGTTGAAAATTCAACAA+GGG 0.606022 tig0004802:-30908 Msa1341240:CDS
TCGCATATTCATGTTGCCAA+GGG 0.608401 tig0004802:+31581 None:intergenic
GAATTTCTGACGGTCTTATG+GGG 0.610921 tig0004802:-31416 Msa1341240:CDS
GAGTGGCTAGACTTTGCGCT+GGG 0.613125 tig0004802:+31829 None:intergenic
CAACATCGATGTCTCTCCGT+CGG 0.614346 tig0004802:-31076 Msa1341240:CDS
ATATCTAGTATCACAACCAA+AGG 0.615031 tig0004802:-31258 Msa1341240:CDS
GTCAACACTAAAGAAGCCTC+GGG 0.618987 tig0004802:-32900 Msa1341240:CDS
AAGTCCTGCTGCTTGAAGAG+TGG 0.619281 tig0004802:+31812 None:intergenic
AACAGTATGATACCTGCAAG+TGG 0.620491 tig0004802:+32102 None:intergenic
GTGTCAAATGGAATTTCTGA+CGG 0.620630 tig0004802:-31426 Msa1341240:CDS
AGCAGAAATCCCGGGATTGG+GGG 0.622120 tig0004802:-31155 Msa1341240:CDS
TTGTATCTTCGAAGGCTGAG+TGG 0.625218 tig0004802:-31963 Msa1341240:CDS
CATCCTGCCCGAAATTATCA+GGG 0.630656 tig0004802:+30839 None:intergenic
AGGTTCCTTTGATCCATGAG+AGG 0.631553 tig0004802:+31756 None:intergenic
CCCTGATGGACTAAAACCGA+CGG 0.643382 tig0004802:+31060 None:intergenic
ATAAATCTGCAAACTCGCGT+AGG 0.643837 tig0004802:-30254 Msa1341240:three_prime_UTR
CAAGGTAATCTCCAAAATGG+TGG 0.644703 tig0004802:-30952 Msa1341240:CDS
ACTGAACCAGCAGAAATCCC+GGG 0.645616 tig0004802:-31163 Msa1341240:CDS
GTTGTCGAATAATTTAGACT+CGG 0.649630 tig0004802:+30520 None:intergenic
TACCTGGAGTGCTTCCAAGA+GGG 0.655640 tig0004802:+31280 None:intergenic
GTTGTGATACTAGATATACC+TGG 0.656765 tig0004802:+31264 None:intergenic
TTCCCTGTGTAGCAGAAGCG+GGG 0.660017 tig0004802:+31556 None:intergenic
TTCAAGCAGCAGGACTTGGT+AGG 0.662359 tig0004802:-31806 Msa1341240:CDS
ACACAGTAGACTGCCCCAAG+AGG 0.664618 tig0004802:+31445 None:intergenic
TTTATGTTGAAAATTCAACA+AGG 0.669918 tig0004802:-30909 Msa1341240:CDS
TAAAGGATTTGCATGCGATG+AGG 0.672320 tig0004802:+31104 None:intergenic
TCTTATGGGGAGAAATAACA+TGG 0.679947 tig0004802:-31403 Msa1341240:CDS
ATACCTGGAGTGCTTCCAAG+AGG 0.686422 tig0004802:+31279 None:intergenic
GTGACAGCAACTATTTACAC+CGG 0.687900 tig0004802:+30581 None:intergenic
TCGACAGACCAAACAACCCG+AGG 0.690366 tig0004802:+32866 None:intergenic
TTGACTGCATAAAATGCACA+AGG 0.702818 tig0004802:-30393 Msa1341240:three_prime_UTR
GTCCCCGCTTCTGCTACACA+GGG 0.706393 tig0004802:-31558 Msa1341240:CDS
ATGGCACAATCCCAACCTAG+AGG 0.710994 tig0004802:-31516 Msa1341240:CDS
TGTGGAACAGGATTAAACGA+AGG 0.721159 tig0004802:+31363 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAATGGTTTTAAAGAAAATT+TGG + tig0004802:30433-30452 None:intergenic 15.0%
!!! TATAATTCTTTTAAAAACAC+TGG + tig0004802:30691-30710 None:intergenic 15.0%
!!! AAAAAATGATTTGTTTGTAA+TGG + tig0004802:32265-32284 None:intergenic 15.0%
!!! CATGTGTAAATATTTTTATT+TGG - tig0004802:32840-32859 Msa1341240:CDS 15.0%
!!! TTATTTGTGATTTTATGTTA+TGG - tig0004802:32979-32998 Msa1341240:CDS 15.0%
!!! ACTATTGATTCATTTCTTAT+TGG - tig0004802:30520-30539 Msa1341240:three_prime_UTR 20.0%
!! ATCTATTATAGAACTAATAG+AGG + tig0004802:30548-30567 None:intergenic 20.0%
!!! ATCAATTCCAGAATTTTTTT+GGG + tig0004802:30828-30847 None:intergenic 20.0%
!!! AGGGATATAAAATTTAACTT+GGG + tig0004802:30931-30950 None:intergenic 20.0%
!! TAGGGATATAAAATTTAACT+TGG + tig0004802:30932-30951 None:intergenic 20.0%
!! TCGTTAAAAATATCATAACT+TGG + tig0004802:31803-31822 None:intergenic 20.0%
!! TTTATGTTGAAAATTCAACA+AGG - tig0004802:32077-32096 Msa1341240:intron 20.0%
!! TTATGTTGAAAATTCAACAA+GGG - tig0004802:32078-32097 Msa1341240:intron 20.0%
!! GAAGAACATAAAATTAAAAG+AGG + tig0004802:32805-32824 None:intergenic 20.0%
! GATCTATCTAAAAGACTATA+TGG - tig0004802:30256-30275 Msa1341240:three_prime_UTR 25.0%
! GAATAATTAAGATTCAGTTG+TGG - tig0004802:30358-30377 Msa1341240:three_prime_UTR 25.0%
! AATCAATAGTAGAACTTCTA+GGG + tig0004802:30510-30529 None:intergenic 25.0%
! CATACAAGAAAAATGTATGA+CGG + tig0004802:30628-30647 None:intergenic 25.0%
!!! ATTCTTTTAAAAACACTGGA+TGG + tig0004802:30687-30706 None:intergenic 25.0%
! AGGTACTCAAACAAATATTA+GGG + tig0004802:30734-30753 None:intergenic 25.0%
!! AAGGTACTCAAACAAATATT+AGG + tig0004802:30735-30754 None:intergenic 25.0%
! AGAAATATCAAACTTGAATC+AGG + tig0004802:30791-30810 None:intergenic 25.0%
!!! CATCAATTCCAGAATTTTTT+TGG + tig0004802:30829-30848 None:intergenic 25.0%
! TTTAATTCCCGAATTAACTT+CGG + tig0004802:31765-31784 None:intergenic 25.0%
!! GTTGAATTTTCAACATAAAG+AGG + tig0004802:32076-32095 None:intergenic 25.0%
! CTCATAATTCATTCTTTCTA+TGG - tig0004802:32205-32224 Msa1341240:intron 25.0%
! TCAAATATTCTTCAAACTTC+TGG - tig0004802:32504-32523 Msa1341240:intron 25.0%
! CAAATATTCTTCAAACTTCT+GGG - tig0004802:32505-32524 Msa1341240:intron 25.0%
!! TCTAGCAATGTTCTAATTTT+AGG - tig0004802:32654-32673 Msa1341240:intron 25.0%
!! CTAGCAATGTTCTAATTTTA+GGG - tig0004802:32655-32674 Msa1341240:intron 25.0%
!! AAAAACTTTTGAAGAGTCCT+GGG + tig0004802:30206-30225 None:intergenic 30.0%
!! AAAAAACTTTTGAAGAGTCC+TGG + tig0004802:30207-30226 None:intergenic 30.0%
ATAGAGAACTAAGAAACTGT+AGG + tig0004802:30484-30503 None:intergenic 30.0%
GAATCAATAGTAGAACTTCT+AGG + tig0004802:30511-30530 None:intergenic 30.0%
TAATAGATTGAACTGCTTCA+AGG - tig0004802:30560-30579 Msa1341240:three_prime_UTR 30.0%
AATAGATTGAACTGCTTCAA+GGG - tig0004802:30561-30580 Msa1341240:three_prime_UTR 30.0%
! TATATCCCTATGTTTTCTTG+TGG - tig0004802:30942-30961 Msa1341240:CDS 30.0%
! AATATCGATTGTATCTTCGA+AGG - tig0004802:31015-31034 Msa1341240:CDS 30.0%
! TTGGGTTTTCAAAACTGAAA+AGG + tig0004802:31253-31272 None:intergenic 30.0%
!!! TTTTCAGTTTTGAAAACCCA+AGG - tig0004802:31252-31271 Msa1341240:CDS 30.0%
AAACCAACAAATCTTCTTCA+CGG - tig0004802:31323-31342 Msa1341240:CDS 30.0%
ATTTCAGTGCTTTGATTCAT+GGG + tig0004802:31671-31690 None:intergenic 30.0%
ATATCTAGTATCACAACCAA+AGG - tig0004802:31728-31747 Msa1341240:CDS 30.0%
ATTTCAAGCCGAAGTTAATT+CGG - tig0004802:31754-31773 Msa1341240:CDS 30.0%
GAAGTTAATTCGGGAATTAA+AGG - tig0004802:31764-31783 Msa1341240:CDS 30.0%
AGGAATCATATGTTAAACCT+TGG + tig0004802:31865-31884 None:intergenic 30.0%
TGAAAATTCAACAAGGGTTA+AGG - tig0004802:32084-32103 Msa1341240:intron 30.0%
GTTGTCGAATAATTTAGACT+CGG + tig0004802:32469-32488 None:intergenic 30.0%
TGTACTATAATGGTTTAGTC+TGG - tig0004802:32533-32552 Msa1341240:intron 30.0%
! TTGTTCTGACTATTCTTTTC+CGG - tig0004802:32876-32895 Msa1341240:CDS 30.0%
GAATTCTTTGTACATGAACA+TGG - tig0004802:32948-32967 Msa1341240:CDS 30.0%
TATTCGTCTTCTGCTAATGA+AGG - tig0004802:30018-30037 Msa1341240:three_prime_UTR 35.0%
CTTCTGCTAATGAAGGAAAT+TGG - tig0004802:30025-30044 Msa1341240:three_prime_UTR 35.0%
! GTCTTTTAGATAGATCTGCA+CGG + tig0004802:30252-30271 None:intergenic 35.0%
GCAATCAAGTTTCATCCTAT+TGG - tig0004802:30394-30413 Msa1341240:three_prime_UTR 35.0%
TCAAGTTTCATCCTATTGGT+TGG - tig0004802:30398-30417 Msa1341240:three_prime_UTR 35.0%
GAGAAAGACAATACGCTAAA+TGG + tig0004802:30450-30469 None:intergenic 35.0%
GACGGAAAATGAATACATTC+AGG + tig0004802:30610-30629 None:intergenic 35.0%
AAACCTTAGCAATCTCATCT+TGG - tig0004802:30657-30676 Msa1341240:CDS 35.0%
!! ACCTTAGCTATGAAGTATTG+TGG - tig0004802:30750-30769 Msa1341240:intron 35.0%
ACCACAATACTTCATAGCTA+AGG + tig0004802:30754-30773 None:intergenic 35.0%
TGGAATTGATGAATGTTCCT+GGG - tig0004802:30838-30857 Msa1341240:CDS 35.0%
GATGACCACAAGAAAACATA+GGG + tig0004802:30950-30969 None:intergenic 35.0%
AGATGACCACAAGAAAACAT+AGG + tig0004802:30951-30970 None:intergenic 35.0%
! GTTTTCTTGTGGTCATCTAA+CGG - tig0004802:30953-30972 Msa1341240:CDS 35.0%
GGCTGAGAAATGAATTATTC+TGG + tig0004802:31061-31080 None:intergenic 35.0%
AAATCCCTTATTGATGCAGA+TGG - tig0004802:31451-31470 Msa1341240:CDS 35.0%
! CCAGTATTTTCACTTAGCAT+TGG + tig0004802:31497-31516 None:intergenic 35.0%
CCAATGCTAAGTGAAAATAC+TGG - tig0004802:31494-31513 Msa1341240:CDS 35.0%
GTGTCAAATGGAATTTCTGA+CGG - tig0004802:31560-31579 Msa1341240:CDS 35.0%
TCTTATGGGGAGAAATAACA+TGG - tig0004802:31583-31602 Msa1341240:CDS 35.0%
CATTTCAGTGCTTTGATTCA+TGG + tig0004802:31672-31691 None:intergenic 35.0%
TCTGTTAGTAATTTCCCTCT+TGG - tig0004802:31692-31711 Msa1341240:CDS 35.0%
GTTGTGATACTAGATATACC+TGG + tig0004802:31725-31744 None:intergenic 35.0%
TTTCAAGCCGAAGTTAATTC+GGG - tig0004802:31755-31774 Msa1341240:CDS 35.0%
! TTTTCTTCTGCTCAACAAAC+CGG - tig0004802:31950-31969 Msa1341240:CDS 35.0%
GAACAAGGTAATCTCCAAAA+TGG - tig0004802:32031-32050 Msa1341240:intron 35.0%
AACCTCTTCCCTGATAATTT+CGG - tig0004802:32139-32158 Msa1341240:CDS 35.0%
!!! TTTTTTGTGTTTCTTGCAGC+AGG - tig0004802:32279-32298 Msa1341240:intron 35.0%
! GAGAATGAGTTTGACTTTGA+CGG - tig0004802:32319-32338 Msa1341240:intron 35.0%
! AGAATGAGTTTGACTTTGAC+GGG - tig0004802:32320-32339 Msa1341240:intron 35.0%
TAACTGCAATCAGAAAGAAC+TGG + tig0004802:32385-32404 None:intergenic 35.0%
TTCTTTCTGATTGCAGTTAC+CGG - tig0004802:32386-32405 Msa1341240:intron 35.0%
TTGACTGCATAAAATGCACA+AGG - tig0004802:32593-32612 Msa1341240:intron 35.0%
!!! ATTTTAGGGTGTTCTTGAAG+AGG - tig0004802:32669-32688 Msa1341240:intron 35.0%
TTCCGGATGTTTATGTTCTT+CGG - tig0004802:32893-32912 Msa1341240:CDS 35.0%
CTTTGTACATGAACATGGTT+TGG - tig0004802:32953-32972 Msa1341240:CDS 35.0%
GAGAAACTCGAGAAAGAGAA+GGG - tig0004802:30047-30066 Msa1341240:three_prime_UTR 40.0%
AAAGAGAAGGGATGATGAAG+AGG - tig0004802:30059-30078 Msa1341240:three_prime_UTR 40.0%
TAGATCTGCACGGAAAACAA+AGG + tig0004802:30242-30261 None:intergenic 40.0%
GGTTTCTGATACCAACCAAT+AGG + tig0004802:30412-30431 None:intergenic 40.0%
GAGATTGCTAAGGTTTGTGA+TGG + tig0004802:30653-30672 None:intergenic 40.0%
GAACCAAGATGAGATTGCTA+AGG + tig0004802:30663-30682 None:intergenic 40.0%
! AGTTTGATATTTCTGCGCAG+AGG - tig0004802:30797-30816 Msa1341240:intron 40.0%
GGCTGTTCCCAAAAAAATTC+TGG - tig0004802:30818-30837 Msa1341240:CDS 40.0%
CTGGAATTGATGAATGTTCC+TGG - tig0004802:30837-30856 Msa1341240:CDS 40.0%
AACAGTATGATACCTGCAAG+TGG + tig0004802:30887-30906 None:intergenic 40.0%
GTTATTCGCCTACTTCAGAA+AGG - tig0004802:30904-30923 Msa1341240:CDS 40.0%
GAAAACCCAAGGTTGAGATT+TGG - tig0004802:31263-31282 Msa1341240:CDS 40.0%
CTTCTCCAAATCTCAACCTT+GGG + tig0004802:31271-31290 None:intergenic 40.0%
CAAGGTTGAGATTTGGAGAA+GGG - tig0004802:31270-31289 Msa1341240:CDS 40.0%
! GGGCAACAACAACTTTTGAA+TGG - tig0004802:31290-31309 Msa1341240:CDS 40.0%
ACTCCGTGAAGAAGATTTGT+TGG + tig0004802:31329-31348 None:intergenic 40.0%
TCGCATATTCATGTTGCCAA+GGG + tig0004802:31408-31427 None:intergenic 40.0%
TGTGCCATCTGCATCAATAA+GGG + tig0004802:31458-31477 None:intergenic 40.0%
TTGTGCCATCTGCATCAATA+AGG + tig0004802:31459-31478 None:intergenic 40.0%
TGGAATTTCTGACGGTCTTA+TGG - tig0004802:31568-31587 Msa1341240:CDS 40.0%
! GGAATTTCTGACGGTCTTAT+GGG - tig0004802:31569-31588 Msa1341240:CDS 40.0%
! GAATTTCTGACGGTCTTATG+GGG - tig0004802:31570-31589 Msa1341240:CDS 40.0%
TGTGGAACAGGATTAAACGA+AGG + tig0004802:31626-31645 None:intergenic 40.0%
TTCAAAAACGAAGAGCTCTG+TGG + tig0004802:31644-31663 None:intergenic 40.0%
TAAAGGATTTGCATGCGATG+AGG + tig0004802:31885-31904 None:intergenic 40.0%
TCGCATGCAAATCCTTTACA+TGG - tig0004802:31887-31906 Msa1341240:CDS 40.0%
ACATCGATGTTGCCATGTAA+AGG + tig0004802:31902-31921 None:intergenic 40.0%
GGAAAATCTCCTTTCTCTCT+TGG - tig0004802:31998-32017 Msa1341240:intron 40.0%
CAAGGTAATCTCCAAAATGG+TGG - tig0004802:32034-32053 Msa1341240:intron 40.0%
!! AATGTTGACTGCCACCATTT+TGG + tig0004802:32048-32067 None:intergenic 40.0%
ATCAGGGAAGAGGTTAGTAT+GGG + tig0004802:32134-32153 None:intergenic 40.0%
TATCAGGGAAGAGGTTAGTA+TGG + tig0004802:32135-32154 None:intergenic 40.0%
ACCTCTTCCCTGATAATTTC+GGG - tig0004802:32140-32159 Msa1341240:CDS 40.0%
GTGACAGCAACTATTTACAC+CGG + tig0004802:32408-32427 None:intergenic 40.0%
CTGGGAGTAGTGTACTATAA+TGG - tig0004802:32523-32542 Msa1341240:intron 40.0%
! TGTTCTTGAAGAGGTATGCT+TGG - tig0004802:32678-32697 Msa1341240:intron 40.0%
! GTTCTTGAAGAGGTATGCTT+GGG - tig0004802:32679-32698 Msa1341240:intron 40.0%
ATAAATCTGCAAACTCGCGT+AGG - tig0004802:32732-32751 Msa1341240:intron 40.0%
GACCGAAGAACATAAACATC+CGG + tig0004802:32898-32917 None:intergenic 40.0%
GGAGAAACTCGAGAAAGAGA+AGG - tig0004802:30046-30065 Msa1341240:three_prime_UTR 45.0%
AGAAGGGATGATGAAGAGGA+AGG - tig0004802:30063-30082 Msa1341240:three_prime_UTR 45.0%
CGTCAACACTAAAGAAGCCT+CGG - tig0004802:30103-30122 Msa1341240:three_prime_UTR 45.0%
GTCAACACTAAAGAAGCCTC+GGG - tig0004802:30104-30123 Msa1341240:three_prime_UTR 45.0%
! TAAAGAAGCCTCGGGTTGTT+TGG - tig0004802:30112-30131 Msa1341240:three_prime_UTR 45.0%
! ACATTTTCTCTAGTGAGCCC+AGG + tig0004802:30858-30877 None:intergenic 45.0%
!! ACTTGGGTCCTTTCTGAAGT+AGG + tig0004802:30915-30934 None:intergenic 45.0%
TTCTTGTGGTCATCTAACGG+AGG - tig0004802:30956-30975 Msa1341240:CDS 45.0%
TTGTATCTTCGAAGGCTGAG+TGG - tig0004802:31023-31042 Msa1341240:CDS 45.0%
TTCATTTCTCAGCCCACAAG+AGG - tig0004802:31067-31086 Msa1341240:CDS 45.0%
TGCCAAATGATGCCTCTTGT+GGG + tig0004802:31082-31101 None:intergenic 45.0%
GGCACGATTTCGTCAATGAA+CGG - tig0004802:31098-31117 Msa1341240:CDS 45.0%
GGTAGGTCAACTGTGAAATC+AGG - tig0004802:31197-31216 Msa1341240:CDS 45.0%
! CTTTGATCCATGAGAGGCAT+TGG + tig0004802:31227-31246 None:intergenic 45.0%
CAATGCCTCTCATGGATCAA+AGG - tig0004802:31225-31244 Msa1341240:CDS 45.0%
AGGTTCCTTTGATCCATGAG+AGG + tig0004802:31233-31252 None:intergenic 45.0%
CCTTCTCCAAATCTCAACCT+TGG + tig0004802:31272-31291 None:intergenic 45.0%
CCAAGGTTGAGATTTGGAGA+AGG - tig0004802:31269-31288 Msa1341240:CDS 45.0%
TCACGGAGTTCCTACTAACA+TGG - tig0004802:31340-31359 Msa1341240:CDS 45.0%
! TGCTTTGGCTCCATGTTAGT+AGG + tig0004802:31353-31372 None:intergenic 45.0%
TGCAAATTGGCGAGTTGCTT+TGG + tig0004802:31368-31387 None:intergenic 45.0%
CTCGCATATTCATGTTGCCA+AGG + tig0004802:31409-31428 None:intergenic 45.0%
ATTTCCCTGTGTAGCAGAAG+CGG + tig0004802:31435-31454 None:intergenic 45.0%
! TAGCATTGGACCTCTAGGTT+GGG + tig0004802:31483-31502 None:intergenic 45.0%
! TTAGCATTGGACCTCTAGGT+TGG + tig0004802:31484-31503 None:intergenic 45.0%
! TCACTTAGCATTGGACCTCT+AGG + tig0004802:31488-31507 None:intergenic 45.0%
TCCAAGATTACCGAACCTCT+TGG - tig0004802:31526-31545 Msa1341240:CDS 45.0%
CCAAGATTACCGAACCTCTT+GGG - tig0004802:31527-31546 Msa1341240:CDS 45.0%
CAAGATTACCGAACCTCTTG+GGG - tig0004802:31528-31547 Msa1341240:CDS 45.0%
! TTCGGCTTGAAATGAGCCTT+TGG + tig0004802:31747-31766 None:intergenic 45.0%
TCGGGAATTAAAGGATCAGC+TGG - tig0004802:31773-31792 Msa1341240:CDS 45.0%
AACTGAACCAGCAGAAATCC+CGG - tig0004802:31822-31841 Msa1341240:CDS 45.0%
!! AGAAGCATCTCTGCTATGTC+CGG + tig0004802:31972-31991 None:intergenic 45.0%
AGCAGAGATGCTTCTTTGCT+TGG - tig0004802:31977-31996 Msa1341240:intron 45.0%
TCTCCTTTCTCTCTTGGAGA+AGG - tig0004802:32004-32023 Msa1341240:intron 45.0%
AAGCCTTCTCCAAGAGAGAA+AGG + tig0004802:32010-32029 None:intergenic 45.0%
TTTCTCTCTTGGAGAAGGCT+TGG - tig0004802:32009-32028 Msa1341240:intron 45.0%
CATCCTGCCCGAAATTATCA+GGG + tig0004802:32150-32169 None:intergenic 45.0%
TCATCCTGCCCGAAATTATC+AGG + tig0004802:32151-32170 None:intergenic 45.0%
!! CATGAGTGCACTTCTGAAAC+AGG - tig0004802:32174-32193 Msa1341240:intron 45.0%
TTGTGTTTCTTGCAGCAGGA+AGG - tig0004802:32283-32302 Msa1341240:intron 45.0%
! TGACTTTGACGGGTATTCTC+TGG - tig0004802:32330-32349 Msa1341240:intron 45.0%
! TGAAGAGGTATGCTTGGGTT+AGG - tig0004802:32684-32703 Msa1341240:intron 45.0%
! GAAGAGGTATGCTTGGGTTA+GGG - tig0004802:32685-32704 Msa1341240:intron 45.0%
CGAGCTCCATCAACAGTTTG+TGG - tig0004802:30140-30159 Msa1341240:three_prime_UTR 50.0%
CAGCAGCCACAAACTGTTGA+TGG + tig0004802:30149-30168 None:intergenic 50.0%
TCAACAGTTTGTGGCTGCTG+TGG - tig0004802:30149-30168 Msa1341240:three_prime_UTR 50.0%
GACAGTATGTATCCTCACCC+AGG - tig0004802:30186-30205 Msa1341240:three_prime_UTR 50.0%
!! CTTTTGAAGAGTCCTGGGTG+AGG + tig0004802:30201-30220 None:intergenic 50.0%
AAATGTTGCCAGCCACTTGC+AGG - tig0004802:30872-30891 Msa1341240:CDS 50.0%
GTATGATACCTGCAAGTGGC+TGG + tig0004802:30883-30902 None:intergenic 50.0%
!! AGCCCACAAGAGGCATCATT+TGG - tig0004802:31077-31096 Msa1341240:CDS 50.0%
GTGCCAAATGATGCCTCTTG+TGG + tig0004802:31083-31102 None:intergenic 50.0%
CAGCTGCTGCAAGAGTTTGA+AGG + tig0004802:31130-31149 None:intergenic 50.0%
CTTCAAACTCTTGCAGCAGC+TGG - tig0004802:31128-31147 Msa1341240:CDS 50.0%
! AAGTCCTGCTGCTTGAAGAG+TGG + tig0004802:31177-31196 None:intergenic 50.0%
ACTCTTCAAGCAGCAGGACT+TGG - tig0004802:31176-31195 Msa1341240:CDS 50.0%
! TTCAAGCAGCAGGACTTGGT+AGG - tig0004802:31180-31199 Msa1341240:CDS 50.0%
AGGCTTACCAATGCCTCTCA+TGG - tig0004802:31217-31236 Msa1341240:CDS 50.0%
TGCTGCAGACTGGTGCAAAT+TGG + tig0004802:31381-31400 None:intergenic 50.0%
TTTCCCTGTGTAGCAGAAGC+GGG + tig0004802:31434-31453 None:intergenic 50.0%
ATGGCACAATCCCAACCTAG+AGG - tig0004802:31470-31489 Msa1341240:CDS 50.0%
! CCCAAGAGGTTCGGTAATCT+TGG + tig0004802:31530-31549 None:intergenic 50.0%
GGGCAGTCTACTGTGTCAAA+TGG - tig0004802:31548-31567 Msa1341240:CDS 50.0%
AACGAAGAGCTCTGTGGAAC+AGG + tig0004802:31638-31657 None:intergenic 50.0%
! TACCTGGAGTGCTTCCAAGA+GGG + tig0004802:31709-31728 None:intergenic 50.0%
! ATACCTGGAGTGCTTCCAAG+AGG + tig0004802:31710-31729 None:intergenic 50.0%
ACTGAACCAGCAGAAATCCC+GGG - tig0004802:31823-31842 Msa1341240:CDS 50.0%
GATTGGGGGATGACAAACCA+AGG - tig0004802:31845-31864 Msa1341240:CDS 50.0%
CAACATCGATGTCTCTCCGT+CGG - tig0004802:31910-31929 Msa1341240:CDS 50.0%
!!! TCCGTCGGTTTTAGTCCATC+AGG - tig0004802:31925-31944 Msa1341240:CDS 50.0%
!! CCCTGATGGACTAAAACCGA+CGG + tig0004802:31929-31948 None:intergenic 50.0%
!!! CCGTCGGTTTTAGTCCATCA+GGG - tig0004802:31926-31945 Msa1341240:CDS 50.0%
GAGCAGAAGAAAAGCCCTGA+TGG + tig0004802:31943-31962 None:intergenic 50.0%
CTTGGAGAAGGCTTGGAACA+AGG - tig0004802:32016-32035 Msa1341240:intron 50.0%
GCCCGAAATTATCAGGGAAG+AGG + tig0004802:32144-32163 None:intergenic 50.0%
CTTCCCTGATAATTTCGGGC+AGG - tig0004802:32144-32163 Msa1341240:CDS 50.0%
! TTCTTGCAGCAGGAAGGCAT+TGG - tig0004802:32289-32308 Msa1341240:intron 50.0%
AGGGCAGAATTGCAGAAGCA+AGG - tig0004802:32704-32723 Msa1341240:intron 50.0%
TCGACAGACCAAACAACCCG+AGG + tig0004802:30123-30142 None:intergenic 55.0%
GTGGCTGCTGTGGATCAACT+AGG - tig0004802:30159-30178 Msa1341240:three_prime_UTR 55.0%
GAGTGGCTAGACTTTGCGCT+GGG + tig0004802:31160-31179 None:intergenic 55.0%
AGAGTGGCTAGACTTTGCGC+TGG + tig0004802:31161-31180 None:intergenic 55.0%
CTAGCCACTCTTCAAGCAGC+AGG - tig0004802:31170-31189 Msa1341240:CDS 55.0%
CAAGGGATTGTGCTGCAGAC+TGG + tig0004802:31391-31410 None:intergenic 55.0%
CAGTCTGCAGCACAATCCCT+TGG - tig0004802:31389-31408 Msa1341240:CDS 55.0%
TTCCCTGTGTAGCAGAAGCG+GGG + tig0004802:31433-31452 None:intergenic 55.0%
GTAGACTGCCCCAAGAGGTT+CGG + tig0004802:31539-31558 None:intergenic 55.0%
ACACAGTAGACTGCCCCAAG+AGG + tig0004802:31544-31563 None:intergenic 55.0%
!! TTCCCTCTTGGAAGCACTCC+AGG - tig0004802:31704-31723 Msa1341240:CDS 55.0%
ACCAGCAGAAATCCCGGGAT+TGG - tig0004802:31828-31847 Msa1341240:CDS 55.0%
CCAGCAGAAATCCCGGGATT+GGG - tig0004802:31829-31848 Msa1341240:CDS 55.0%
CAGCAGAAATCCCGGGATTG+GGG - tig0004802:31830-31849 Msa1341240:CDS 55.0%
AGCAGAAATCCCGGGATTGG+GGG - tig0004802:31831-31850 Msa1341240:CDS 55.0%
GTTTGTCATCCCCCAATCCC+GGG + tig0004802:31843-31862 None:intergenic 55.0%
GGTTTGTCATCCCCCAATCC+CGG + tig0004802:31844-31863 None:intergenic 55.0%
TTAGTATGGGAGCTCGCGTC+AGG + tig0004802:32121-32140 None:intergenic 55.0%
!! CAGCAGGAAGGCATTGGACA+AGG - tig0004802:32295-32314 Msa1341240:intron 55.0%
GGCGAGAGTCTCTTCTAGAC+AGG + tig0004802:32364-32383 None:intergenic 55.0%
CTAGACTTTGCGCTGGGAGC+TGG + tig0004802:31154-31173 None:intergenic 60.0%
AGTCCCCGCTTCTGCTACAC+AGG - tig0004802:31427-31446 Msa1341240:CDS 60.0%
GTCCCCGCTTCTGCTACACA+GGG - tig0004802:31428-31447 Msa1341240:CDS 60.0%
CCCAATCCCGGGATTTCTGC+TGG + tig0004802:31832-31851 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
tig0004802 gene 30006 33002 30006 ID=Msa1341240;Name=Msa1341240
tig0004802 mRNA 30006 33002 30006 ID=Msa1341240-mRNA-1;Parent=Msa1341240;Name=Msa1341240-mRNA-1;_AED=0.05;_eAED=0.05;_QI=0|1|0.75|1|1|1|4|633|504
tig0004802 exon 30006 30710 30006 ID=Msa1341240-mRNA-1:exon:3055;Parent=Msa1341240-mRNA-1
tig0004802 exon 30813 31994 30813 ID=Msa1341240-mRNA-1:exon:3054;Parent=Msa1341240-mRNA-1
tig0004802 exon 32115 32191 32115 ID=Msa1341240-mRNA-1:exon:3053;Parent=Msa1341240-mRNA-1
tig0004802 exon 32819 33002 32819 ID=Msa1341240-mRNA-1:exon:3052;Parent=Msa1341240-mRNA-1
tig0004802 CDS 32819 33002 32819 ID=Msa1341240-mRNA-1:cds;Parent=Msa1341240-mRNA-1
tig0004802 CDS 32115 32191 32115 ID=Msa1341240-mRNA-1:cds;Parent=Msa1341240-mRNA-1
tig0004802 CDS 30813 31994 30813 ID=Msa1341240-mRNA-1:cds;Parent=Msa1341240-mRNA-1
tig0004802 CDS 30639 30710 30639 ID=Msa1341240-mRNA-1:cds;Parent=Msa1341240-mRNA-1
tig0004802 three_prime_UTR 30006 30638 30006 ID=Msa1341240-mRNA-1:three_prime_utr;Parent=Msa1341240-mRNA-1
Gene Sequence

>Msa1341240

GATGATGCTGATTATTCGTCTTCTGCTAATGAAGGAAATTGGAGAAACTCGAGAAAGAGAAGGGATGATGAAGAGGAAGGTGACGATAGAGATGACTCGTCAACACTAAAGAAGCCTCGGGTTGTTTGGTCTGTCGAGCTCCATCAACAGTTTGTGGCTGCTGTGGATCAACTAGGAATTGACAAGGCTGTTCCCAAAAAAATTCTGGAATTGATGAATGTTCCTGGGCTCACTAGAGAAAATGTTGCCAGCCACTTGCAGAAATATCGATTGTATCTTCGAAGGCTGAGTGGAGTTTCTCAGCACCAGAATAATTCATTTCTCAGCCCACAAGAGGCATCATTTGGCACGATTTCGTCAATGAACGGACTTGACCTTCAAACTCTTGCAGCAGCTGGCCAGCTCCCAGCGCAAAGTCTAGCCACTCTTCAAGCAGCAGGACTTGGTAGGTCAACTGTGAAATCAGGCTTACCAATGCCTCTCATGGATCAAAGGAACCTTTTCAGTTTTGAAAACCCAAGGTTGAGATTTGGAGAAGGGCAACAACAACTTTTGAATGGTAGTAATAATAAACCAACAAATCTTCTTCACGGAGTTCCTACTAACATGGAGCCAAAGCAACTCGCCAATTTGCACCAGTCTGCAGCACAATCCCTTGGCAACATGAATATGCGAGTCCCCGCTTCTGCTACACAGGGAAATCCCTTATTGATGCAGATGGCACAATCCCAACCTAGAGGTCCAATGCTAAGTGAAAATACTGGTTCTCATGTTCCAAGATTACCGAACCTCTTGGGGCAGTCTACTGTGTCAAATGGAATTTCTGACGGTCTTATGGGGAGAAATAACATGGCTAGTAGCAGTAGAGCGCCTTCGTTTAATCCTGTTCCACAGAGCTCTTCGTTTTTGAATTTTCCCATGAATCAAAGCACTGAAATGTCTGTTAGTAATTTCCCTCTTGGAAGCACTCCAGGTATATCTAGTATCACAACCAAAGGCTCATTTCAAGCCGAAGTTAATTCGGGAATTAAAGGATCAGCTGGATTTCCAAGTTATGATATTTTTAACGAACTGAACCAGCAGAAATCCCGGGATTGGGGGATGACAAACCAAGGTTTAACATATGATTCCTCATCGCATGCAAATCCTTTACATGGCAACATCGATGTCTCTCCGTCGGTTTTAGTCCATCAGGGCTTTTCTTCTGCTCAACAAACCGGACATAGCAGAGATGCTTCTTTGCTTGGAAAATCTCCTTTCTCTCTTGGAGAAGGCTTGGAACAAGGTAATCTCCAAAATGGTGGCAGTCAACATTTCAATCCTCTTTATGTTGAAAATTCAACAAGGGTTAAGGCTGAAAGAGTTCCTGACGCGAGCTCCCATACTAACCTCTTCCCTGATAATTTCGGGCAGGATGATCTCATGAGTGCACTTCTGAAACAGCAGGAAGGCATTGGACAAGGCGAGAATGAGTTTGACTTTGACGGGTATTCTCTGGACAACATTCCTGTCTAG
Protein sequence

>Msa1341240

DDADYSSSANEGNWRNSRKRRDDEEEGDDRDDSSTLKKPRVVWSVELHQQFVAAVDQLGIDKAVPKKILELMNVPGLTRENVASHLQKYRLYLRRLSGVSQHQNNSFLSPQEASFGTISSMNGLDLQTLAAAGQLPAQSLATLQAAGLGRSTVKSGLPMPLMDQRNLFSFENPRLRFGEGQQQLLNGSNNKPTNLLHGVPTNMEPKQLANLHQSAAQSLGNMNMRVPASATQGNPLLMQMAQSQPRGPMLSENTGSHVPRLPNLLGQSTVSNGISDGLMGRNNMASSSRAPSFNPVPQSSSFLNFPMNQSTEMSVSNFPLGSTPGISSITTKGSFQAEVNSGIKGSAGFPSYDIFNELNQQKSRDWGMTNQGLTYDSSSHANPLHGNIDVSPSVLVHQGFSSAQQTGHSRDASLLGKSPFSLGEGLEQGNLQNGGSQHFNPLYVENSTRVKAERVPDASSHTNLFPDNFGQDDLMSALLKQQEGIGQGENEFDFDGYSLDNIPV*