AlfalfaGEDB | Msa1342220

M. sativa cultivar zhongmu-4 / Msa1342220

KEGG: K08065
GO: GO:0003674 GO:0003700 GO:0006355 GO:0008150 GO:0009889 GO:0010468 GO:0010556 GO:0019219 GO:0019222 GO:0031323 GO:0031326 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0060255 GO:0065007 GO:0080090 GO:0140110 GO:1903506 GO:2000112 GO:2001141
PFAM: PF00808.26

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1342220	XP_003630291.1	95.455	220	4	3	1	220	1	214	2.95e-150	426

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1342220	sp\|Q9SIT9\|NFYB7_ARATH	67.391	184	47	6	45	218	35	215	3.60e-71	219

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1342220	G7LE45	95.455	220	4	3	1	220	1	214	1.41e-150	426

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TFs/TRs:

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Gene ID	Type	Classification
Msa1342220	TF	NF-YB

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
No data available in table

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1342220	MtrunA17_Chr8g0384451	95.455	220	4	3	1	220	1	214	2.72e-154	426
Msa1342220	MtrunA17_Chr3g0102351	76.364	110	26	0	32	141	13	122	1.62e-60	188
Msa1342220	MtrunA17_Chr5g0446491	75.221	113	28	0	28	140	5	117	9.72e-60	186
Msa1342220	MtrunA17_Chr2g0290491	78.704	108	21	1	45	152	16	121	4.55e-59	183
Msa1342220	MtrunA17_Chr8g0382931	73.913	115	30	0	40	154	22	136	3.57e-58	182
Msa1342220	MtrunA17_Chr7g0263601	70.270	111	32	1	30	139	9	119	5.13e-57	178
Msa1342220	MtrunA17_Chr4g0076321	77.660	94	21	0	47	140	20	113	9.58e-54	169
Msa1342220	MtrunA17_Chr1g0185911	72.917	96	26	0	40	135	23	118	5.95e-53	168
Msa1342220	MtrunA17_Chr1g0195851	66.964	112	37	0	29	140	19	130	1.19e-52	168
Msa1342220	MtrunA17_Chr2g0296321	66.990	103	34	0	45	147	65	167	3.53e-49	161

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1342220	AT2G13570.1	67.391	184	47	6	45	218	35	215	3.66e-72	219
Msa1342220	AT4G14540.1	72.951	122	32	1	27	147	1	122	3.07e-64	196
Msa1342220	AT5G47640.1	73.276	116	30	1	43	158	24	138	8.08e-60	186
Msa1342220	AT3G53340.5	56.081	148	56	2	45	185	28	173	3.22e-55	174
Msa1342220	AT3G53340.1	56.081	148	56	2	45	185	28	173	3.22e-55	174
Msa1342220	AT2G37060.2	78.947	95	20	0	45	139	29	123	3.64e-55	174
Msa1342220	AT2G37060.3	78.947	95	20	0	45	139	29	123	3.64e-55	174
Msa1342220	AT2G37060.1	78.947	95	20	0	45	139	29	123	3.64e-55	174
Msa1342220	AT2G38880.3	68.571	105	33	0	35	139	10	114	2.09e-54	171
Msa1342220	AT2G38880.2	68.571	105	33	0	35	139	10	114	2.59e-54	171

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Find 22 sgRNAs with CRISPR-Local

Find 37 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AACAACAAAGATCTGAACAA+AGG	0.635439	tig0005289:-67250	Msa1342220:CDS
AAGATCAGATTGATTCCATA+GGG	0.573421	tig0005289:-67058	Msa1342220:CDS
ACATTTATCCGATGCTTCCC+CGG	0.490805	tig0005289:+67415	None:intergenic
AGATTGATTCCATAGGGCAT+TGG	0.544180	tig0005289:-67052	Msa1342220:CDS
ATGGAATCAATCTGATCTTG+CGG	0.627874	tig0005289:+67062	None:intergenic
ATTCTACCAACATTTGCTAT+AGG	0.386839	tig0005289:+67530	None:intergenic
ATTTGGGCTATTACAACGTT+AGG	0.507634	tig0005289:-67353	Msa1342220:CDS
CAAGATCAGATTGATTCCAT+AGG	0.513351	tig0005289:-67059	Msa1342220:CDS
CAATGGTGATGATATTATTT+GGG	0.366036	tig0005289:-67369	Msa1342220:CDS
CATAGTAATAATTTGCCAAA+TGG	0.423743	tig0005289:-67668	Msa1342220:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
TTTCTTCCTATAGCAAATGT+TGG	-	tig0005289:67190-67209	Msa1342220:CDS	30.0%
TGCAAATGGAAAAATCTCAA+AGG	-	tig0005289:67237-67256	Msa1342220:CDS	30.0%
AACAACAAAGATCTGAACAA+AGG	-	tig0005289:67476-67495	Msa1342220:CDS	30.0%
ATTTGGGCTATTACAACGTT+AGG	-	tig0005289:67373-67392	Msa1342220:CDS	35.0%
TGATCAGTAGTCATATAAGG+AGG	+	tig0005289:67595-67614	None:intergenic	35.0%
GGTTGATCAGTAGTCATATA+AGG	+	tig0005289:67598-67617	None:intergenic	35.0%
TGAATGGAATTTGGAGAGAA+AGG	+	tig0005289:67631-67650	None:intergenic	35.0%
ATGGAATCAATCTGATCTTG+CGG	+	tig0005289:67667-67686	None:intergenic	35.0%
GACAGTTCAAGAATGTGTGT+CGG	-	tig0005289:67270-67289	Msa1342220:CDS	40.0%
GAATTTGGAGAGAAAGGAAG+TGG	+	tig0005289:67625-67644	None:intergenic	40.0%

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Chromosome	Type	Strat	End	Strand	Name
tig0005289	gene	67043	67705	67043	ID=Msa1342220;Name=Msa1342220
tig0005289	mRNA	67043	67705	67043	ID=Msa1342220-mRNA-1;Parent=Msa1342220;Name=Msa1342220-mRNA-1;_AED=0.00;_eAED=0.00;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|220
tig0005289	exon	67043	67705	67043	ID=Msa1342220-mRNA-1:exon:3503;Parent=Msa1342220-mRNA-1
tig0005289	CDS	67043	67705	67043	ID=Msa1342220-mRNA-1:cds;Parent=Msa1342220-mRNA-1

Gene Sequence

>Msa1342220

ATGGATGATGAGAGTCATAGTAATAATTTGCCAAATGGATTCAACACAGAAAACCCTGAAAGTCATTGTTTAAAAACAATCAACAACAACAACAACAATAATCATAATCATCATCACAATAGCAATAGCAATAAAGAACAAGATCGTTTTCTTCCTATAGCAAATGTTGGTAGAATAATGAAAAAAGTTATTCCTGCAAATGGAAAAATCTCAAAGGATGCAAAAGAGACAGTTCAAGAATGTGTGTCGGAGTTCATAAGTTTTGTCACCGGGGAAGCATCGGATAAATGTCAAAGAGAAAAAAGAAAGACAATCAATGGTGATGATATTATTTGGGCTATTACAACGTTAGGCTTTGAAGATTATGTGGAACCATTAAAATGTTACCTTCAAAAATATAGAGATATTGAAGGTGAAAAAGTCAATGTTCCAAAACAACAAAGATCTGAACAAAGGCTACATCAACATCAACATCAACATAATCAAGATGAATTAAATAATCAACATTTCAACAATAGTGTATATACATCTACAAATCTCATGTCTCAACCTCCTTATATGACTACTGATCAACCATTTCCACTTCCTTTCTCTCCAAATTCCATTCAAAAACAATTACGACCGCAAGATCAGATTGATTCCATAGGGCATTGGTATGAATGA

Protein sequence

>Msa1342220

MDDESHSNNLPNGFNTENPESHCLKTINNNNNNNHNHHHNSNSNKEQDRFLPIANVGRIMKKVIPANGKISKDAKETVQECVSEFISFVTGEASDKCQREKRKTINGDDIIWAITTLGFEDYVEPLKCYLQKYRDIEGEKVNVPKQQRSEQRLHQHQHQHNQDELNNQHFNNSVYTSTNLMSQPPYMTTDQPFPLPFSPNSIQKQLRPQDQIDSIGHWYE*