AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1344580

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1344580 MtrunA17_Chr1g0206771 91.463 164 14 0 1 164 1 164 1.33e-110 313
Msa1344580 MtrunA17_Chr1g0206781 49.600 125 44 1 6 130 1 106 4.68e-33 114
Msa1344580 MtrunA17_Chr1g0206781 61.905 84 31 1 94 177 10 92 3.23e-27 99.8
Msa1344580 MtrunA17_Chr3g0077911 45.806 155 74 3 13 160 5 156 1.69e-32 114
Msa1344580 MtrunA17_Chr3g0077911 46.835 79 38 2 96 173 9 84 1.98e-12 62.4
Msa1344580 MtrunA17_Chr5g0415031 44.737 152 77 2 13 157 40 191 5.48e-32 121
Msa1344580 MtrunA17_Chr5g0415031 51.351 74 36 0 96 169 44 117 5.92e-17 78.6
Msa1344580 MtrunA17_Chr3g0077861 47.742 155 71 4 13 160 5 156 5.73e-32 113
Msa1344580 MtrunA17_Chr1g0205821 38.372 172 98 2 5 168 2 173 2.57e-31 112
Msa1344580 MtrunA17_Chr1g0205821 40.964 83 49 0 92 174 10 92 2.84e-12 62.4
Msa1344580 MtrunA17_Chr3g0077891 40.476 168 75 3 17 165 9 170 1.48e-30 110
Msa1344580 MtrunA17_Chr3g0077891 51.389 72 34 1 9 79 92 163 5.45e-16 72.4
Msa1344580 MtrunA17_Chr3g0077941 41.104 163 84 2 13 163 5 167 7.96e-30 108
Msa1344580 MtrunA17_Chr8g0364231 41.509 159 86 2 6 157 32 190 1.25e-29 115
Msa1344580 MtrunA17_Chr8g0364231 55.405 74 33 0 96 169 43 116 4.00e-19 84.7
Msa1344580 MtrunA17_Chr1g0205831 37.500 160 91 2 16 174 2 153 2.25e-29 107
Msa1344580 MtrunA17_Chr6g0452001 42.208 154 78 3 13 157 60 211 5.82e-29 113
Msa1344580 MtrunA17_Chr6g0452001 50.667 75 37 0 96 170 64 138 3.46e-17 79.0
Msa1344580 MtrunA17_Chr4g0075861 41.139 158 79 2 15 163 88 240 2.54e-28 108
Msa1344580 MtrunA17_Chr4g0075861 42.667 150 78 3 17 163 13 157 3.02e-24 97.8
Msa1344580 MtrunA17_Chr4g0075861 41.176 85 47 1 91 175 8 89 4.84e-11 60.8
Msa1344580 MtrunA17_Chr4g0075971 40.506 158 80 2 15 163 88 240 2.72e-27 107
Msa1344580 MtrunA17_Chr4g0075971 42.857 154 76 3 15 163 11 157 6.25e-25 101
Msa1344580 MtrunA17_Chr4g0075971 43.038 79 42 1 91 169 8 83 1.90e-11 62.4
Msa1344580 MtrunA17_Chr3g0077881 40.854 164 84 4 13 163 5 168 4.92e-27 101
Msa1344580 MtrunA17_Chr3g0077881 45.783 83 44 1 96 177 9 91 8.37e-13 63.9
Msa1344580 MtrunA17_Chr6g0452021 41.333 150 83 1 13 157 40 189 2.92e-26 104
Msa1344580 MtrunA17_Chr1g0209201 44.961 129 64 3 19 145 19 142 8.57e-26 97.1
Msa1344580 MtrunA17_Chr1g0209201 50.685 73 35 1 92 164 13 84 2.19e-17 75.1
Msa1344580 MtrunA17_Chr1g0205861 50.617 81 40 0 1 81 1 81 9.80e-21 83.2
Msa1344580 MtrunA17_Chr1g0205861 39.806 103 56 1 92 194 13 109 2.40e-17 74.3
Msa1344580 MtrunA17_Chr7g0218891 36.129 155 90 3 17 162 100 254 1.08e-20 86.7
Msa1344580 MtrunA17_Chr7g0218891 33.516 182 109 5 1 173 1 179 7.73e-20 84.3
Msa1344580 MtrunA17_Chr7g0256011 48.193 83 43 0 1 83 1 83 1.55e-19 80.1
Msa1344580 MtrunA17_Chr1g0205811 52.055 73 35 0 16 88 14 86 1.35e-18 77.8
Msa1344580 MtrunA17_Chr1g0205811 48.387 93 44 2 95 184 14 105 1.94e-17 74.7
Msa1344580 MtrunA17_Chr8g0364021 48.611 72 37 0 9 80 37 108 2.06e-15 72.0
Msa1344580 MtrunA17_Chr8g0364021 40.816 98 54 1 96 193 45 138 2.00e-12 63.9
Msa1344580 MtrunA17_Chr1g0205801 43.836 73 41 0 16 88 12 84 5.49e-14 65.5
Msa1344580 MtrunA17_Chr1g0205801 45.000 80 44 0 95 174 12 91 1.24e-12 61.6
Msa1344580 MtrunA17_Chr2g0328701 41.584 101 56 1 69 169 3 100 5.57e-14 65.9
Msa1344580 MtrunA17_Chr2g0328701 40.777 103 53 3 15 114 28 125 6.26e-13 62.8
Msa1344580 MtrunA17_Chr7g0218911 49.180 61 31 0 20 80 2 62 7.69e-14 65.1
Msa1344580 MtrunA17_Chr3g0077811 46.479 71 37 1 93 162 11 81 2.79e-12 61.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1344580 AT1G24190.1 42.581 155 76 4 13 157 11 162 1.09e-30 118
Msa1344580 AT1G24190.1 55.882 68 30 0 96 163 15 82 9.35e-18 81.3
Msa1344580 AT1G24190.3 42.581 155 76 4 13 157 11 162 1.14e-30 118
Msa1344580 AT1G24190.3 55.882 68 30 0 96 163 15 82 9.36e-18 81.3
Msa1344580 AT1G24190.2 42.581 155 76 4 13 157 11 162 1.21e-30 118
Msa1344580 AT1G24190.2 55.882 68 30 0 96 163 15 82 9.62e-18 81.3
Msa1344580 AT3G01320.1 42.384 151 81 2 13 157 54 204 2.62e-30 117
Msa1344580 AT3G01320.1 55.405 74 33 0 96 169 58 131 2.27e-18 82.8
Msa1344580 AT3G01320.2 42.384 151 81 2 13 157 54 204 3.21e-30 117
Msa1344580 AT3G01320.2 55.405 74 33 0 96 169 58 131 2.38e-18 82.8
Msa1344580 AT5G15020.1 42.105 152 79 2 13 157 49 198 7.21e-30 115
Msa1344580 AT5G15020.1 53.521 71 33 0 96 166 53 123 4.02e-17 79.3
Msa1344580 AT5G15020.2 42.105 152 79 2 13 157 49 198 7.85e-30 115
Msa1344580 AT5G15020.2 53.521 71 33 0 96 166 53 123 4.09e-17 79.3
Msa1344580 AT1G24230.1 34.524 168 95 3 13 179 11 164 1.61e-29 109
Msa1344580 AT1G24230.1 26.174 149 100 3 13 154 83 228 2.57e-13 66.6
Msa1344580 AT1G70060.2 40.994 161 83 2 7 157 5 163 2.69e-29 114
Msa1344580 AT1G70060.2 54.545 77 35 0 96 172 15 91 2.57e-18 82.8
Msa1344580 AT1G70060.1 40.994 161 83 2 7 157 5 163 2.69e-29 114
Msa1344580 AT1G70060.1 54.545 77 35 0 96 172 15 91 2.57e-18 82.8
Msa1344580 AT1G24200.1 37.333 150 90 1 12 157 10 159 3.31e-28 105
Msa1344580 AT1G24200.1 50.000 68 34 0 96 163 15 82 1.41e-14 69.3
Msa1344580 AT1G27260.1 36.538 156 84 2 14 156 1 154 9.16e-27 102
Msa1344580 AT1G10450.3 39.264 163 83 3 7 157 72 230 1.54e-25 103
Msa1344580 AT1G10450.3 48.529 68 35 0 96 163 85 152 1.25e-12 66.2
Msa1344580 AT1G10450.2 39.264 163 83 3 7 157 21 179 1.61e-25 103
Msa1344580 AT1G10450.2 48.529 68 35 0 96 163 34 101 1.44e-12 65.9
Msa1344580 AT1G10450.1 39.264 163 83 3 7 157 21 179 1.61e-25 103
Msa1344580 AT1G10450.1 48.529 68 35 0 96 163 34 101 1.44e-12 65.9
Msa1344580 AT1G59890.4 41.060 151 74 3 17 157 46 191 3.95e-25 102
Msa1344580 AT1G59890.4 50.000 74 37 0 96 169 46 119 3.69e-15 73.6
Msa1344580 AT1G59890.3 41.060 151 74 3 17 157 46 191 3.95e-25 102
Msa1344580 AT1G59890.3 50.000 74 37 0 96 169 46 119 3.49e-15 73.6
Msa1344580 AT1G59890.2 41.060 151 74 3 17 157 46 191 4.23e-25 102
Msa1344580 AT1G59890.2 50.000 74 37 0 96 169 46 119 3.56e-15 73.6
Msa1344580 AT1G59890.1 41.060 151 74 3 17 157 46 191 4.27e-25 102
Msa1344580 AT1G59890.1 50.000 74 37 0 96 169 46 119 3.62e-15 73.6
Msa1344580 AT1G70030.1 35.762 151 90 2 13 157 8 157 5.10e-23 90.5
Msa1344580 AT1G70030.1 44.118 68 38 0 99 166 15 82 4.57e-13 64.3
Msa1344580 AT1G24250.1 29.843 191 105 3 1 164 1 189 6.89e-21 87.4
Msa1344580 AT1G23810.1 32.184 174 102 3 7 164 5 178 1.32e-20 86.3
Msa1344580 AT5G15025.1 55.882 68 30 0 13 80 44 111 2.11e-19 80.1
Msa1344580 AT5G15025.1 42.857 77 44 0 94 170 46 122 3.69e-14 66.6
Msa1344580 AT4G12020.2 59.701 67 26 1 13 79 305 370 2.57e-18 82.8
Msa1344580 AT4G12020.2 46.667 75 39 1 95 169 308 381 4.59e-15 73.2
Msa1344580 AT4G12020.4 59.701 67 26 1 13 79 305 370 2.60e-18 82.8
Msa1344580 AT4G12020.4 46.667 75 39 1 95 169 308 381 4.63e-15 73.2
Msa1344580 AT4G12020.1 59.701 67 26 1 13 79 305 370 2.61e-18 82.8
Msa1344580 AT4G12020.1 46.667 75 39 1 95 169 308 381 4.62e-15 73.2
Msa1344580 AT4G12020.9 59.701 67 26 1 13 79 305 370 2.61e-18 82.8
Msa1344580 AT4G12020.9 46.667 75 39 1 95 169 308 381 4.62e-15 73.2
Msa1344580 AT4G12020.7 59.701 67 26 1 13 79 305 370 2.82e-18 82.8
Msa1344580 AT4G12020.7 46.667 75 39 1 95 169 308 381 4.94e-15 73.2
Msa1344580 AT4G12020.5 59.701 67 26 1 13 79 238 303 2.82e-18 82.8
Msa1344580 AT4G12020.5 46.667 75 39 1 95 169 241 314 4.94e-15 73.2
Msa1344580 AT4G12020.3 59.701 67 26 1 13 79 305 370 3.09e-18 82.4
Msa1344580 AT4G12020.3 46.667 75 39 1 95 169 308 381 5.25e-15 73.2
Msa1344580 AT4G12020.10 59.701 67 26 1 13 79 305 370 3.24e-18 82.4
Msa1344580 AT4G12020.10 46.667 75 39 1 95 169 308 381 5.61e-15 73.2
Msa1344580 AT4G12020.6 59.701 67 26 1 13 79 305 370 3.51e-18 82.4
Msa1344580 AT4G12020.6 46.667 75 39 1 95 169 308 381 5.97e-15 73.2
Msa1344580 AT4G12020.8 59.701 67 26 1 13 79 305 370 3.51e-18 82.4
Msa1344580 AT4G12020.8 46.667 75 39 1 95 169 308 381 5.97e-15 73.2
Msa1344580 AT5G15040.1 52.174 69 33 0 14 82 1 69 4.06e-18 75.9
Msa1344580 AT5G15040.1 43.590 78 44 0 93 170 1 78 5.63e-13 62.4
Msa1344580 AT5G15040.3 52.174 69 33 0 14 82 1 69 4.06e-18 75.9
Msa1344580 AT5G15040.3 43.590 78 44 0 93 170 1 78 5.63e-13 62.4
Msa1344580 AT5G15040.2 52.174 69 33 0 14 82 1 69 4.06e-18 75.9
Msa1344580 AT5G15040.2 43.590 78 44 0 93 170 1 78 5.63e-13 62.4
Msa1344580 AT4G12020.11 59.701 67 26 1 13 79 305 370 4.99e-18 82.0
Msa1344580 AT4G12020.11 46.667 75 39 1 95 169 308 381 8.15e-15 72.4
Msa1344580 AT1G70030.2 36.000 125 72 2 1 117 7 131 2.40e-16 72.8
Msa1344580 AT1G70030.2 44.118 68 38 0 99 166 26 93 2.26e-13 64.7
Msa1344580 AT1G27250.1 43.750 80 43 1 2 79 5 84 4.17e-16 72.0
Msa1344580 AT1G27250.1 42.857 77 44 0 86 162 12 88 9.31e-13 63.2
Msa1344580 AT1G70030.3 38.384 99 55 1 13 105 19 117 1.32e-15 70.5
Msa1344580 AT1G70030.3 44.118 68 38 0 99 166 26 93 1.38e-13 65.1
Msa1344580 AT1G27220.1 46.575 73 37 1 9 79 5 77 3.82e-15 70.5
Msa1344580 AT1G27220.1 42.308 78 45 0 86 163 5 82 3.17e-13 65.5
Msa1344580 AT1G27270.1 33.537 164 85 3 15 156 13 174 1.16e-14 70.5
Msa1344580 AT1G27280.1 29.371 143 92 1 28 161 5 147 2.08e-13 66.6
Msa1344580 AT1G27280.1 39.394 66 40 0 13 78 78 143 3.54e-11 60.5
Msa1344580 AT1G27240.1 39.437 71 43 0 12 82 10 80 1.33e-12 63.9

Find 31 sgRNAs with CRISPR-Local

Find 34 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGAATTTGTGAAAGCTATTA+AGG 0.207434 tig0006809:+45809 Msa1344580:CDS
AGATTGAAGGATTTGTTTAA+AGG 0.287517 tig0006809:+45636 Msa1344580:CDS
TAATCAAACTTGCTCCCTTT+TGG 0.325065 tig0006809:-45696 None:intergenic
AAAGGGAGCAAGTTTGATTA+TGG 0.329129 tig0006809:+45699 Msa1344580:CDS
GTACTGATTTGATATTTCTT+TGG 0.342104 tig0006809:-45933 None:intergenic
AGAGTGAAGGAACTATTTAA+AGG 0.356818 tig0006809:+45873 Msa1344580:CDS
GATTGAAGGATTTGTTTAAA+GGG 0.359025 tig0006809:+45637 Msa1344580:CDS
AAATGTTATGATGGATTTCA+AGG 0.397261 tig0006809:+45581 Msa1344580:CDS
GCTTCTTCCAACACTCCAAA+TGG 0.411742 tig0006809:-45729 None:intergenic
GGGCATACAAATTTAACGTT+GGG 0.437947 tig0006809:+45894 Msa1344580:CDS
GATTATGGCATAGATCCATT+TGG 0.439528 tig0006809:+45714 Msa1344580:CDS
TTCAACATCTTCGTGCCAAA+AGG 0.444915 tig0006809:+45681 Msa1344580:CDS
GAGTGAAGGAACTATTTAAA+GGG 0.467701 tig0006809:+45874 Msa1344580:CDS
CATAGATCCATTTGGAGTGT+TGG 0.482305 tig0006809:+45722 Msa1344580:CDS
TATCAAATCAGTACTGATGA+TGG 0.498943 tig0006809:+45942 Msa1344580:CDS
TCAACATCTTCGTGCCAAAA+GGG 0.534332 tig0006809:+45682 Msa1344580:CDS
TCCATGAGACATGTTATCGC+TGG 0.536428 tig0006809:-45469 None:intergenic
TGGTGTCATAGCAAGATTGA+AGG 0.543726 tig0006809:+45623 Msa1344580:CDS
AGGGCATACAAATTTAACGT+TGG 0.547701 tig0006809:+45893 Msa1344580:CDS
TCCAGCGATAACATGTCTCA+TGG 0.552003 tig0006809:+45468 Msa1344580:CDS
AGTTGAGTTTCAATATGAGA+GGG 0.554210 tig0006809:+45776 Msa1344580:CDS
TGACGTAGAAGCAGAAGACA+TGG 0.554579 tig0006809:+46019 Msa1344580:CDS
GCTATTGCTTCTTCCATCGT+GGG 0.554911 tig0006809:-45498 None:intergenic
GAAAGCTATTAAGGATTGCA+AGG 0.560574 tig0006809:+45818 Msa1344580:CDS
TGCTATTGCTTCTTCCATCG+TGG 0.562428 tig0006809:-45499 None:intergenic
AAGTTGAGTTTCAATATGAG+AGG 0.581901 tig0006809:+45775 Msa1344580:CDS
AGAAGCAGAAGACATGGAAG+TGG 0.582532 tig0006809:+46025 Msa1344580:CDS
ATGTCTCGCGAAAAGAGTGA+AGG 0.647795 tig0006809:+45860 Msa1344580:CDS
GCTATGAAAATTGATACAAG+TGG 0.667045 tig0006809:+45603 Msa1344580:CDS
AGAAGCAATAGCATATGTCG+AGG 0.681954 tig0006809:+45509 Msa1344580:CDS
TCATGGAGAAGTTCCCACGA+TGG 0.689488 tig0006809:+45485 Msa1344580:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TGAATTTTTAAATGTTATGA+TGG + tig0006809:45572-45591 Msa1344580:CDS 15.0%
!!! AAATGTTATGATGGATTTCA+AGG + tig0006809:45581-45600 Msa1344580:CDS 25.0%
!! AGATTGAAGGATTTGTTTAA+AGG + tig0006809:45636-45655 Msa1344580:CDS 25.0%
!! GATTGAAGGATTTGTTTAAA+GGG + tig0006809:45637-45656 Msa1344580:CDS 25.0%
! TGAATTTGTGAAAGCTATTA+AGG + tig0006809:45809-45828 Msa1344580:CDS 25.0%
!!! GTACTGATTTGATATTTCTT+TGG - tig0006809:45936-45955 None:intergenic 25.0%
GCTATGAAAATTGATACAAG+TGG + tig0006809:45603-45622 Msa1344580:CDS 30.0%
!! GGGCACACAAATTTAATTTT+AGG + tig0006809:45657-45676 Msa1344580:CDS 30.0%
AAGTTGAGTTTCAATATGAG+AGG + tig0006809:45775-45794 Msa1344580:CDS 30.0%
AGTTGAGTTTCAATATGAGA+GGG + tig0006809:45776-45795 Msa1344580:CDS 30.0%
AGAGTGAAGGAACTATTTAA+AGG + tig0006809:45873-45892 Msa1344580:CDS 30.0%
GAGTGAAGGAACTATTTAAA+GGG + tig0006809:45874-45893 Msa1344580:CDS 30.0%
!! TATCAAATCAGTACTGATGA+TGG + tig0006809:45942-45961 Msa1344580:CDS 30.0%
!! TAATCAAACTTGCTCCCTTT+TGG - tig0006809:45699-45718 None:intergenic 35.0%
AAAGGGAGCAAGTTTGATTA+TGG + tig0006809:45699-45718 Msa1344580:CDS 35.0%
GATTATGGCATAGATCCATT+TGG + tig0006809:45714-45733 Msa1344580:CDS 35.0%
! GAAACTCAACTTTTACTGCA+TGG - tig0006809:45767-45786 None:intergenic 35.0%
GAAAGCTATTAAGGATTGCA+AGG + tig0006809:45818-45837 Msa1344580:CDS 35.0%
AGGGCATACAAATTTAACGT+TGG + tig0006809:45893-45912 Msa1344580:CDS 35.0%
GGGCATACAAATTTAACGTT+GGG + tig0006809:45894-45913 Msa1344580:CDS 35.0%
AGAAGCAATAGCATATGTCG+AGG + tig0006809:45509-45528 Msa1344580:CDS 40.0%
!! TGGTGTCATAGCAAGATTGA+AGG + tig0006809:45623-45642 Msa1344580:CDS 40.0%
TTCAACATCTTCGTGCCAAA+AGG + tig0006809:45681-45700 Msa1344580:CDS 40.0%
TCAACATCTTCGTGCCAAAA+GGG + tig0006809:45682-45701 Msa1344580:CDS 40.0%
! CATAGATCCATTTGGAGTGT+TGG + tig0006809:45722-45741 Msa1344580:CDS 40.0%
TCCAGCGATAACATGTCTCA+TGG + tig0006809:45468-45487 Msa1344580:CDS 45.0%
TCCATGAGACATGTTATCGC+TGG - tig0006809:45472-45491 None:intergenic 45.0%
GCTATTGCTTCTTCCATCGT+GGG - tig0006809:45501-45520 None:intergenic 45.0%
TGCTATTGCTTCTTCCATCG+TGG - tig0006809:45502-45521 None:intergenic 45.0%
GCTTCTTCCAACACTCCAAA+TGG - tig0006809:45732-45751 None:intergenic 45.0%
ATGTCTCGCGAAAAGAGTGA+AGG + tig0006809:45860-45879 Msa1344580:CDS 45.0%
TGACGTAGAAGCAGAAGACA+TGG + tig0006809:46019-46038 Msa1344580:CDS 45.0%
AGAAGCAGAAGACATGGAAG+TGG + tig0006809:46025-46044 Msa1344580:CDS 45.0%
TCATGGAGAAGTTCCCACGA+TGG + tig0006809:45485-45504 Msa1344580:CDS 50.0%
Chromosome Type Strat End Strand Name
tig0006809 gene 45465 46052 45465 ID=Msa1344580;Name=Msa1344580
tig0006809 mRNA 45465 46052 45465 ID=Msa1344580-mRNA-1;Parent=Msa1344580;Name=Msa1344580-mRNA-1;_AED=0.05;_eAED=0.05;_QI=0|-1|0|1|-1|1|1|0|195
tig0006809 exon 45465 46052 45465 ID=Msa1344580-mRNA-1:exon:4605;Parent=Msa1344580-mRNA-1
tig0006809 CDS 45465 46052 45465 ID=Msa1344580-mRNA-1:cds;Parent=Msa1344580-mRNA-1
Gene Sequence

>Msa1344580

ATGTCCAGCGATAACATGTCTCATGGAGAAGTTCCCACGATGGAAGAAGCAATAGCATATGTCGAGGCTGTAAAAGACGAGTTTCAAGATGAGATAGAAAAGTATGATGAATTTTTAAATGTTATGATGGATTTCAAGGCTATGAAAATTGATACAAGTGGTGTCATAGCAAGATTGAAGGATTTGTTTAAAGGGCACACAAATTTAATTTTAGGATTCAACATCTTCGTGCCAAAAGGGAGCAAGTTTGATTATGGCATAGATCCATTTGGAGTGTTGGAAGAAGCAAAAGCATATGTCCATGCAGTAAAAGTTGAGTTTCAATATGAGAGGGAAAAGTATGATGAATTTGTGAAAGCTATTAAGGATTGCAAGGCTGAGAGAATTGATACAAAATGTCTCGCGAAAAGAGTGAAGGAACTATTTAAAGGGCATACAAATTTAACGTTGGGATTTAACAACTTTCTGCCAAAGAAATATCAAATCAGTACTGATGATGGTGATGATGATGATGCTGAAGATGATGATGATGATGATGATGATGATGATGAGACTGACGTAGAAGCAGAAGACATGGAAGTGGATTAA
Protein sequence

>Msa1344580

MSSDNMSHGEVPTMEEAIAYVEAVKDEFQDEIEKYDEFLNVMMDFKAMKIDTSGVIARLKDLFKGHTNLILGFNIFVPKGSKFDYGIDPFGVLEEAKAYVHAVKVEFQYEREKYDEFVKAIKDCKAERIDTKCLAKRVKELFKGHTNLTLGFNNFLPKKYQISTDDGDDDDAEDDDDDDDDDDETDVEAEDMEVD*