AlfalfaGEDB | Msa1345440

M. sativa cultivar zhongmu-4 / Msa1345440

KEGG: -
GO: GO:0001666 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004712 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005789 GO:0006082 GO:0006464 GO:0006468 GO:0006629 GO:0006720 GO:0006721 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0008299 GO:0008610 GO:0009058 GO:0009628 GO:0009685 GO:0009686 GO:0009719 GO:0009723 GO:0009725 GO:0009743 GO:0009744 GO:0009746 GO:0009750 GO:0009756 GO:0009966 GO:0009968 GO:0009987 GO:0010033 GO:0010035 GO:0010038 GO:0010039 GO:0010104 GO:0010105 GO:0010182 GO:0010646 GO:0010648 GO:0010817 GO:0012505 GO:0016020 GO:0016053 GO:0016101 GO:0016102 GO:0016114 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0019752 GO:0023051 GO:0023052 GO:0023057 GO:0031984 GO:0034284 GO:0034285 GO:0036211 GO:0036293 GO:0040034 GO:0042175 GO:0042221 GO:0042445 GO:0042446 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043436 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044260 GO:0044267 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044425 GO:0044432 GO:0044444 GO:0044446 GO:0044464 GO:0046394 GO:0046777 GO:0048506 GO:0048509 GO:0048510 GO:0048519 GO:0048523 GO:0048580 GO:0048583 GO:0048585 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051239 GO:0051302 GO:0051716 GO:0065007 GO:0065008 GO:0070297 GO:0070298 GO:0070482 GO:0070887 GO:0071241 GO:0071248 GO:0071281 GO:0071310 GO:0071322 GO:0071704 GO:0098827 GO:0140096 GO:1901564 GO:1901576 GO:1901700 GO:1901701 GO:1902531 GO:1902532 GO:2000026 GO:2000035 GO:2000069 GO:2000280
PFAM: PF07714.20

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1345440	ABN07953.1	100.000	91	0	0	1	91	192	282	3.84e-59	192

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1345440	sp\|Q9FPR3\|EDR1_ARATH	58.571	70	29	0	1	70	857	926	3.85e-23	94.7

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1345440	A2Q2V9	100.000	91	0	0	1	91	192	282	1.83e-59	192

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1345440	MtrunA17_Chr2g0294351	100.000	91	0	0	1	91	654	744	4.32e-60	194
Msa1345440	MtrunA17_Chr4g0068411	76.667	90	21	0	1	90	648	737	2.94e-38	134
Msa1345440	MtrunA17_Chr1g0151541	57.534	73	31	0	1	73	851	923	3.68e-26	100
Msa1345440	MtrunA17_Chr1g0191171	54.545	77	35	0	1	77	877	953	2.25e-24	95.1
Msa1345440	MtrunA17_Chr6g0482711	57.143	70	30	0	1	70	848	917	4.48e-24	94.4
Msa1345440	MtrunA17_Chr8g0392871	47.778	90	39	1	1	82	927	1016	1.77e-23	92.8
Msa1345440	MtrunA17_Chr5g0397931	53.846	65	30	0	1	65	611	675	5.70e-23	91.3
Msa1345440	MtrunA17_Chr1g0179331	50.000	68	34	0	1	68	745	812	3.03e-20	83.6
Msa1345440	MtrunA17_Chr3g0132791	50.000	68	34	0	1	68	843	910	4.05e-19	80.1
Msa1345440	MtrunA17_Chr1g0171451	47.059	68	36	0	1	68	604	671	1.82e-18	78.2
Msa1345440	MtrunA17_Chr5g0427651	45.205	73	40	0	1	73	836	908	1.01e-17	76.3
Msa1345440	MtrunA17_Chr7g0244621	39.706	68	41	0	1	68	242	309	1.05e-14	67.4
Msa1345440	MtrunA17_Chr6g0461841	36.765	68	43	0	1	68	1166	1233	8.97e-13	62.4
Msa1345440	MtrunA17_Chr4g0065211	35.294	68	44	0	1	68	1320	1387	1.83e-12	61.2
Msa1345440	MtrunA17_Chr4g0072741	36.765	68	43	0	1	68	1034	1101	4.21e-12	60.5
Msa1345440	MtrunA17_Chr2g0305141	32.468	77	52	0	1	77	1282	1358	6.20e-12	59.7
Msa1345440	MtrunA17_Chr1g0196631	33.824	68	45	0	1	68	1099	1166	8.68e-12	59.3
Msa1345440	MtrunA17_Chr3g0143531	35.714	70	45	0	1	70	210	279	1.23e-11	58.5
Msa1345440	MtrunA17_Chr1g0211611	35.714	70	45	0	1	70	486	555	9.77e-11	56.2

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1345440	AT3G06640.2	74.419	86	22	0	1	86	634	719	1.59e-42	147
Msa1345440	AT3G06640.3	74.419	86	22	0	1	86	634	719	1.59e-42	147
Msa1345440	AT3G06640.1	77.500	80	18	0	1	80	634	713	1.77e-41	144
Msa1345440	AT5G49470.1	67.816	87	28	0	1	87	392	478	2.15e-41	141
Msa1345440	AT5G49470.7	67.816	87	28	0	1	87	552	638	4.19e-41	142
Msa1345440	AT1G67890.1	70.732	82	24	0	1	82	674	755	4.42e-41	143
Msa1345440	AT5G49470.3	67.816	87	28	0	1	87	679	765	5.66e-41	142
Msa1345440	AT3G06620.1	73.171	82	22	0	1	82	682	763	5.75e-41	142
Msa1345440	AT5G49470.4	67.816	87	28	0	1	87	653	739	6.48e-41	142
Msa1345440	AT5G49470.2	67.816	87	28	0	1	87	740	826	7.58e-41	142
Msa1345440	AT3G06630.2	76.000	75	18	0	1	75	622	696	3.80e-37	131
Msa1345440	AT5G11850.1	60.870	69	27	0	1	69	797	865	1.48e-25	99.0
Msa1345440	AT4G23050.1	53.521	71	33	0	1	71	655	725	2.84e-24	95.1
Msa1345440	AT4G23050.2	53.521	71	33	0	1	71	656	726	2.95e-24	95.1
Msa1345440	AT1G08720.1	58.571	70	29	0	1	70	857	926	3.92e-24	94.7
Msa1345440	AT1G67890.4	75.510	49	12	0	1	49	546	594	4.29e-24	94.7
Msa1345440	AT1G67890.3	75.510	49	12	0	1	49	674	722	6.61e-24	94.4
Msa1345440	AT1G67890.2	75.510	49	12	0	1	49	674	722	6.61e-24	94.4
Msa1345440	AT3G06630.1	79.592	49	10	0	1	49	622	670	8.06e-24	94.0
Msa1345440	AT1G18160.1	48.889	90	44	1	1	90	903	990	1.77e-23	93.2
Msa1345440	AT1G73660.1	51.429	70	34	0	1	70	936	1005	3.63e-21	86.7
Msa1345440	AT5G03730.2	50.000	68	34	0	1	68	741	808	1.15e-18	79.3
Msa1345440	AT5G03730.1	50.000	68	34	0	1	68	741	808	1.15e-18	79.3
Msa1345440	AT1G08720.2	58.000	50	21	0	1	50	857	906	6.88e-16	71.6
Msa1345440	AT4G24480.3	44.118	68	38	0	1	68	859	926	7.45e-16	71.2
Msa1345440	AT4G24480.1	44.118	68	38	0	1	68	864	931	8.30e-16	71.2
Msa1345440	AT4G24480.2	44.118	68	38	0	1	68	859	926	8.80e-16	71.2
Msa1345440	AT1G18160.2	56.000	50	22	0	1	50	903	952	3.39e-14	66.6
Msa1345440	AT5G01850.1	36.765	68	43	0	1	68	215	282	7.43e-13	62.8
Msa1345440	AT5G01850.2	36.765	68	43	0	1	68	239	306	8.76e-13	62.4
Msa1345440	AT1G04700.1	35.294	68	44	0	1	68	965	1032	1.55e-12	62.0
Msa1345440	AT1G04700.2	35.294	68	44	0	1	68	965	1032	1.55e-12	62.0
Msa1345440	AT4G35780.1	34.524	84	55	0	1	84	477	560	3.65e-12	60.8
Msa1345440	AT5G57610.1	35.294	68	44	0	1	68	980	1047	5.97e-12	60.1

Find 43 sgRNAs with CRISPR-Local

Find 170 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GGTCCGACGTTTACAGCTTT+GGG	0.248911	tig0007163:+125	Msa1345440:intron
ACTATTAAGTTTAAGATCTA+TGG	0.271950	tig0007163:-1069	None:intergenic
AGGTCCGACGTTTACAGCTT+TGG	0.324983	tig0007163:+124	Msa1345440:intron
AATTCATAAACCTCTGATAA+TGG	0.333663	tig0007163:-1266	None:intergenic
CTCGCGGCGGCTGATGTATA+AGG	0.338415	tig0007163:+927	Msa1345440:three_prime_UTR
GAACCATCGGCTAGAAATTC+CGG	0.360246	tig0007163:+465	Msa1345440:CDS
ACAAAATAACTACAATAGTT+TGG	0.364744	tig0007163:-1042	None:intergenic
ACCAACATTCCAGGAACTTC+TGG	0.366306	tig0007163:+781	Msa1345440:CDS
TAATAGTGCTGCTCTATGTT+TGG	0.373735	tig0007163:+1085	Msa1345440:three_prime_UTR
TAAGCCTTTCCAGAAGTTCC+TGG	0.388590	tig0007163:-790	None:intergenic
GGCTCTCCGACCAACATTCC+AGG	0.393570	tig0007163:+772	Msa1345440:CDS
CTTCTATAATCGAGAGTTGT+TGG	0.404809	tig0007163:+509	Msa1345440:CDS
CACCAGCAGATCTGGCTGCT+TGG	0.434818	tig0007163:-844	None:intergenic
CACTGTGGATCAATATCTTC+CGG	0.442758	tig0007163:-484	None:intergenic
TTCCAAGCAGCCAGATCTGC+TGG	0.459522	tig0007163:+842	Msa1345440:CDS
GTATATTACTATCAGTGATC+CGG	0.474943	tig0007163:+751	Msa1345440:intron
TTGCAGGTTATTGGAGCTGT+AGG	0.490152	tig0007163:+436	Msa1345440:intron
TCCAGAAGTTCCTGGAATGT+TGG	0.492115	tig0007163:-782	None:intergenic
TGGAATGTTGGTCGGAGAGC+CGG	0.496889	tig0007163:-770	None:intergenic
CTTCCGGAATTTCTAGCCGA+TGG	0.500022	tig0007163:-468	None:intergenic
GTCCGACGTTTACAGCTTTG+GGG	0.504812	tig0007163:+126	Msa1345440:five_prime_UTR
AGCAGCCAGATCTGCTGGTG+GGG	0.516345	tig0007163:+847	Msa1345440:CDS
ACAGCTTTGGGGTGATAATG+TGG	0.526146	tig0007163:+137	Msa1345440:exon
CAGCTTTGGGGTGATAATGT+GGG	0.539435	tig0007163:+138	Msa1345440:exon
TTTGGCAAGATTTAATGTTG+TGG	0.541679	tig0007163:-1024	None:intergenic
CATTCCAGGAACTTCTGGAA+AGG	0.573284	tig0007163:+786	Msa1345440:CDS
GATGAGTTGGTACAAACAGC+TGG	0.574675	tig0007163:+991	Msa1345440:three_prime_UTR
CTGTAGGATTTATGAACCAT+CGG	0.575199	tig0007163:+452	Msa1345440:CDS
GGACACTCTCAATGCAATGC+AGG	0.576007	tig0007163:+186	Msa1345440:CDS
TCTGGCTGCTTGGAACTGAA+TGG	0.582787	tig0007163:-834	None:intergenic
TTCCTCATGAGAAGATGAGT+TGG	0.584410	tig0007163:+978	Msa1345440:three_prime_UTR
TACCAACTCATCTTCTCATG+AGG	0.594831	tig0007163:-980	None:intergenic
CGGAAGATATTGATCCACAG+TGG	0.605158	tig0007163:+485	Msa1345440:CDS
GAAGTTCCTGGAATGTTGGT+CGG	0.609876	tig0007163:-778	None:intergenic
AAGCAGCCAGATCTGCTGGT+GGG	0.611552	tig0007163:+846	Msa1345440:CDS
GGAAGATATTGATCCACAGT+GGG	0.614299	tig0007163:+486	Msa1345440:CDS
CTCTCAAAGATGGATCAGCT+AGG	0.621875	tig0007163:-888	None:intergenic
GGCTTAAAGAACTTCAGAGA+CGG	0.624923	tig0007163:+807	Msa1345440:CDS
TTGCATTGAGAGTGTCCCAA+GGG	0.640217	tig0007163:-180	None:intergenic
CAAGCAGCCAGATCTGCTGG+TGG	0.652620	tig0007163:+845	Msa1345440:CDS
ATTGCATTGAGAGTGTCCCA+AGG	0.679627	tig0007163:-181	None:intergenic
CACCCCAAAGCTGTAAACGT+CGG	0.692604	tig0007163:-128	None:intergenic
TCGATTATAGAAGCCCACTG+TGG	0.723770	tig0007163:-499	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	CTTTTAATATTAAACATTAT+TGG	+	tig0007163:700-719	Msa1345440:intron	10.0%
!!!	CTTTTAATATTAAACATTAT+TGG	+	tig0007163:700-719	Msa1345440:intron	10.0%
!!!	TTTAATTTTATAAATTCCTG+TGG	-	tig0007163:241-260	None:intergenic	15.0%
!!	AAAACAAAATATACATATCA+TGG	+	tig0007163:627-646	Msa1345440:intron	15.0%
!!!	CATTGTAATGTAATTTTATA+TGG	+	tig0007163:1141-1160	Msa1345440:three_prime_UTR	15.0%
!!!	TTTAATTTTATAAATTCCTG+TGG	-	tig0007163:241-260	None:intergenic	15.0%
!!	AAAACAAAATATACATATCA+TGG	+	tig0007163:627-646	Msa1345440:intron	15.0%
!!!	CATTGTAATGTAATTTTATA+TGG	+	tig0007163:1141-1160	Msa1345440:three_prime_UTR	15.0%
!!!	TATTTTGTTTTTTACACCAA+AGG	-	tig0007163:618-637	None:intergenic	20.0%
!!	ATATTAAAAGTAAGTGAAGT+TGG	-	tig0007163:690-709	None:intergenic	20.0%
!!	ACAAAATAACTACAATAGTT+TGG	-	tig0007163:1045-1064	None:intergenic	20.0%
!!!	ACTATTAAGTTTAAGATCTA+TGG	-	tig0007163:1072-1091	None:intergenic	20.0%
!!!	ACCATTTTTAATTGTACAAA+AGG	+	tig0007163:1175-1194	Msa1345440:three_prime_UTR	20.0%
!!!	ACCTTTTGTACAATTAAAAA+TGG	-	tig0007163:1179-1198	None:intergenic	20.0%
!!!	TATTTTGTTTTTTACACCAA+AGG	-	tig0007163:618-637	None:intergenic	20.0%
!!	ATATTAAAAGTAAGTGAAGT+TGG	-	tig0007163:690-709	None:intergenic	20.0%
!!	ACAAAATAACTACAATAGTT+TGG	-	tig0007163:1045-1064	None:intergenic	20.0%
!!!	ACTATTAAGTTTAAGATCTA+TGG	-	tig0007163:1072-1091	None:intergenic	20.0%
!!!	ACCATTTTTAATTGTACAAA+AGG	+	tig0007163:1175-1194	Msa1345440:three_prime_UTR	20.0%
!!!	ACCTTTTGTACAATTAAAAA+TGG	-	tig0007163:1179-1198	None:intergenic	20.0%
!	TTGTTACTAAAATTCCACTT+TGG	+	tig0007163:73-92	Msa1345440:intron	25.0%
!	CAAAATGGATCATTATAATG+TGG	+	tig0007163:312-331	Msa1345440:intron	25.0%
!!	GATTTTCACATTTCTTTCAA+TGG	+	tig0007163:649-668	Msa1345440:intron	25.0%
!	AAAGAAAAAACTCTCAAAGA+TGG	-	tig0007163:901-920	None:intergenic	25.0%
!	TACAAAAGGTAGAGAAAAAA+AGG	+	tig0007163:1189-1208	Msa1345440:three_prime_UTR	25.0%
!!	ATAATGGAACTAAAAAGTGT+GGG	-	tig0007163:1253-1272	None:intergenic	25.0%
!	AATTCATAAACCTCTGATAA+TGG	-	tig0007163:1269-1288	None:intergenic	25.0%
!	TTGTTACTAAAATTCCACTT+TGG	+	tig0007163:73-92	Msa1345440:intron	25.0%
!	CAAAATGGATCATTATAATG+TGG	+	tig0007163:312-331	Msa1345440:intron	25.0%
!!	GATTTTCACATTTCTTTCAA+TGG	+	tig0007163:649-668	Msa1345440:intron	25.0%
!	AAAGAAAAAACTCTCAAAGA+TGG	-	tig0007163:901-920	None:intergenic	25.0%
!	TACAAAAGGTAGAGAAAAAA+AGG	+	tig0007163:1189-1208	Msa1345440:three_prime_UTR	25.0%
!!	ATAATGGAACTAAAAAGTGT+GGG	-	tig0007163:1253-1272	None:intergenic	25.0%
!	AATTCATAAACCTCTGATAA+TGG	-	tig0007163:1269-1288	None:intergenic	25.0%
!	TATAATGATCCATTTTGCAG+CGG	-	tig0007163:309-328	None:intergenic	30.0%
!	ACAGTTTCTCTAATTTTTGC+AGG	+	tig0007163:420-439	Msa1345440:intron	30.0%
!	CTCTAATTTTTGCAGGTTAT+TGG	+	tig0007163:427-446	Msa1345440:intron	30.0%
!!	TTTTTACACCAAAGGTTGAT+AGG	-	tig0007163:610-629	None:intergenic	30.0%
	GTATATTACTATCAGTGATC+CGG	+	tig0007163:751-770	Msa1345440:intron	30.0%
!!!	TTTTTTCTTTTTGTCACTCG+CGG	+	tig0007163:911-930	Msa1345440:three_prime_UTR	30.0%
	TTTGGCAAGATTTAATGTTG+TGG	-	tig0007163:1027-1046	None:intergenic	30.0%
!!	GATAATGGAACTAAAAAGTG+TGG	-	tig0007163:1254-1273	None:intergenic	30.0%
!!!	CTTTTTAGTTCCATTATCAG+AGG	+	tig0007163:1256-1275	Msa1345440:three_prime_UTR	30.0%
!	TATAATGATCCATTTTGCAG+CGG	-	tig0007163:309-328	None:intergenic	30.0%
!	ACAGTTTCTCTAATTTTTGC+AGG	+	tig0007163:420-439	Msa1345440:intron	30.0%
!	CTCTAATTTTTGCAGGTTAT+TGG	+	tig0007163:427-446	Msa1345440:intron	30.0%
!!	TTTTTACACCAAAGGTTGAT+AGG	-	tig0007163:610-629	None:intergenic	30.0%
	GTATATTACTATCAGTGATC+CGG	+	tig0007163:751-770	Msa1345440:intron	30.0%
!!!	TTTTTTCTTTTTGTCACTCG+CGG	+	tig0007163:911-930	Msa1345440:three_prime_UTR	30.0%
	TTTGGCAAGATTTAATGTTG+TGG	-	tig0007163:1027-1046	None:intergenic	30.0%
!!	GATAATGGAACTAAAAAGTG+TGG	-	tig0007163:1254-1273	None:intergenic	30.0%
!!!	CTTTTTAGTTCCATTATCAG+AGG	+	tig0007163:1256-1275	Msa1345440:three_prime_UTR	30.0%
	CATACACACTTCTCATTTGA+GGG	-	tig0007163:15-34	None:intergenic	35.0%
	GCACAAAAAGATATCCAAAG+TGG	-	tig0007163:90-109	None:intergenic	35.0%
!!!	GATATCTTTTTGTGCTGCTT+GGG	+	tig0007163:95-114	Msa1345440:intron	35.0%
	TTGCAACAGAAAAAATCCCT+TGG	+	tig0007163:164-183	Msa1345440:CDS	35.0%
	TGCAACAGAAAAAATCCCTT+GGG	+	tig0007163:165-184	Msa1345440:CDS	35.0%
	TTCATATGTACATGAACCAC+AGG	+	tig0007163:222-241	Msa1345440:intron	35.0%
	AATGAAAAACCGCTGCAAAA+TGG	+	tig0007163:297-316	Msa1345440:intron	35.0%
!	GCAGTTTTTACTATCATCTC+AGG	+	tig0007163:334-353	Msa1345440:intron	35.0%
	GAAACATAACAACAGGCATT+TGG	-	tig0007163:362-381	None:intergenic	35.0%
	AGACAGTGAAACATAACAAC+AGG	-	tig0007163:369-388	None:intergenic	35.0%
	CTGTAGGATTTATGAACCAT+CGG	+	tig0007163:452-471	Msa1345440:CDS	35.0%
	CTTCTATAATCGAGAGTTGT+TGG	+	tig0007163:509-528	Msa1345440:CDS	35.0%
!!	AAAGGTTGATAGGATGTGTT+TGG	-	tig0007163:600-619	None:intergenic	35.0%
	AAACACATCCTATCAACCTT+TGG	+	tig0007163:599-618	Msa1345440:intron	35.0%
!	TAATAGTGCTGCTCTATGTT+TGG	+	tig0007163:1085-1104	Msa1345440:three_prime_UTR	35.0%
	CATACACACTTCTCATTTGA+GGG	-	tig0007163:15-34	None:intergenic	35.0%
	GCACAAAAAGATATCCAAAG+TGG	-	tig0007163:90-109	None:intergenic	35.0%
!!!	GATATCTTTTTGTGCTGCTT+GGG	+	tig0007163:95-114	Msa1345440:intron	35.0%
	TTGCAACAGAAAAAATCCCT+TGG	+	tig0007163:164-183	Msa1345440:CDS	35.0%
	TGCAACAGAAAAAATCCCTT+GGG	+	tig0007163:165-184	Msa1345440:CDS	35.0%
	TTCATATGTACATGAACCAC+AGG	+	tig0007163:222-241	Msa1345440:intron	35.0%
	AATGAAAAACCGCTGCAAAA+TGG	+	tig0007163:297-316	Msa1345440:intron	35.0%
!	GCAGTTTTTACTATCATCTC+AGG	+	tig0007163:334-353	Msa1345440:intron	35.0%
	GAAACATAACAACAGGCATT+TGG	-	tig0007163:362-381	None:intergenic	35.0%
	AGACAGTGAAACATAACAAC+AGG	-	tig0007163:369-388	None:intergenic	35.0%
	CTGTAGGATTTATGAACCAT+CGG	+	tig0007163:452-471	Msa1345440:CDS	35.0%
	CTTCTATAATCGAGAGTTGT+TGG	+	tig0007163:509-528	Msa1345440:CDS	35.0%
!!	AAAGGTTGATAGGATGTGTT+TGG	-	tig0007163:600-619	None:intergenic	35.0%
	AAACACATCCTATCAACCTT+TGG	+	tig0007163:599-618	Msa1345440:intron	35.0%
!	TAATAGTGCTGCTCTATGTT+TGG	+	tig0007163:1085-1104	Msa1345440:three_prime_UTR	35.0%
	TCTCATTTGAGGGTTCGTTA+CGG	-	tig0007163:5-24	None:intergenic	40.0%
	GCATACACACTTCTCATTTG+AGG	-	tig0007163:16-35	None:intergenic	40.0%
	TAGAGAGATAGCGCAATTGA+GGG	-	tig0007163:46-65	None:intergenic	40.0%
	ATAGAGAGATAGCGCAATTG+AGG	-	tig0007163:47-66	None:intergenic	40.0%
!!	GGATATCTTTTTGTGCTGCT+TGG	+	tig0007163:94-113	Msa1345440:intron	40.0%
	CACTGTGGATCAATATCTTC+CGG	-	tig0007163:487-506	None:intergenic	40.0%
	GGAAGATATTGATCCACAGT+GGG	+	tig0007163:486-505	Msa1345440:CDS	40.0%
	GGCTTAAAGAACTTCAGAGA+CGG	+	tig0007163:807-826	Msa1345440:CDS	40.0%
	TTCCTCATGAGAAGATGAGT+TGG	+	tig0007163:978-997	Msa1345440:three_prime_UTR	40.0%
	TACCAACTCATCTTCTCATG+AGG	-	tig0007163:983-1002	None:intergenic	40.0%
	TCTCATTTGAGGGTTCGTTA+CGG	-	tig0007163:5-24	None:intergenic	40.0%
	GCATACACACTTCTCATTTG+AGG	-	tig0007163:16-35	None:intergenic	40.0%
	TAGAGAGATAGCGCAATTGA+GGG	-	tig0007163:46-65	None:intergenic	40.0%
	ATAGAGAGATAGCGCAATTG+AGG	-	tig0007163:47-66	None:intergenic	40.0%
!!	GGATATCTTTTTGTGCTGCT+TGG	+	tig0007163:94-113	Msa1345440:intron	40.0%
	CACTGTGGATCAATATCTTC+CGG	-	tig0007163:487-506	None:intergenic	40.0%
	GGAAGATATTGATCCACAGT+GGG	+	tig0007163:486-505	Msa1345440:CDS	40.0%
	GGCTTAAAGAACTTCAGAGA+CGG	+	tig0007163:807-826	Msa1345440:CDS	40.0%
	TTCCTCATGAGAAGATGAGT+TGG	+	tig0007163:978-997	Msa1345440:three_prime_UTR	40.0%
	TACCAACTCATCTTCTCATG+AGG	-	tig0007163:983-1002	None:intergenic	40.0%
!!	TTGTGCTGCTTGGGAAATTC+AGG	+	tig0007163:104-123	Msa1345440:intron	45.0%
!!	ACAGCTTTGGGGTGATAATG+TGG	+	tig0007163:137-156	Msa1345440:exon	45.0%
!!	CAGCTTTGGGGTGATAATGT+GGG	+	tig0007163:138-157	Msa1345440:exon	45.0%
!!	TTGCATTGAGAGTGTCCCAA+GGG	-	tig0007163:183-202	None:intergenic	45.0%
!!	ATTGCATTGAGAGTGTCCCA+AGG	-	tig0007163:184-203	None:intergenic	45.0%
	TTGCAGGTTATTGGAGCTGT+AGG	+	tig0007163:436-455	Msa1345440:intron	45.0%
!	GAACCATCGGCTAGAAATTC+CGG	+	tig0007163:465-484	Msa1345440:CDS	45.0%
	CGGAAGATATTGATCCACAG+TGG	+	tig0007163:485-504	Msa1345440:CDS	45.0%
	TCGATTATAGAAGCCCACTG+TGG	-	tig0007163:502-521	None:intergenic	45.0%
	GAAGTTCCTGGAATGTTGGT+CGG	-	tig0007163:781-800	None:intergenic	45.0%
	ACCAACATTCCAGGAACTTC+TGG	+	tig0007163:781-800	Msa1345440:CDS	45.0%
	TCCAGAAGTTCCTGGAATGT+TGG	-	tig0007163:785-804	None:intergenic	45.0%
!	CATTCCAGGAACTTCTGGAA+AGG	+	tig0007163:786-805	Msa1345440:CDS	45.0%
	TAAGCCTTTCCAGAAGTTCC+TGG	-	tig0007163:793-812	None:intergenic	45.0%
	CTCTCAAAGATGGATCAGCT+AGG	-	tig0007163:891-910	None:intergenic	45.0%
!	TTTCTTTTTGTCACTCGCGG+CGG	+	tig0007163:914-933	Msa1345440:three_prime_UTR	45.0%
!!	GATGAGTTGGTACAAACAGC+TGG	+	tig0007163:991-1010	Msa1345440:three_prime_UTR	45.0%
!!	TTGTGCTGCTTGGGAAATTC+AGG	+	tig0007163:104-123	Msa1345440:intron	45.0%
!!	ACAGCTTTGGGGTGATAATG+TGG	+	tig0007163:137-156	Msa1345440:exon	45.0%
!!	CAGCTTTGGGGTGATAATGT+GGG	+	tig0007163:138-157	Msa1345440:exon	45.0%
!!	TTGCATTGAGAGTGTCCCAA+GGG	-	tig0007163:183-202	None:intergenic	45.0%
!!	ATTGCATTGAGAGTGTCCCA+AGG	-	tig0007163:184-203	None:intergenic	45.0%
	TTGCAGGTTATTGGAGCTGT+AGG	+	tig0007163:436-455	Msa1345440:intron	45.0%
!	GAACCATCGGCTAGAAATTC+CGG	+	tig0007163:465-484	Msa1345440:CDS	45.0%
	CGGAAGATATTGATCCACAG+TGG	+	tig0007163:485-504	Msa1345440:CDS	45.0%
	TCGATTATAGAAGCCCACTG+TGG	-	tig0007163:502-521	None:intergenic	45.0%
	GAAGTTCCTGGAATGTTGGT+CGG	-	tig0007163:781-800	None:intergenic	45.0%
	ACCAACATTCCAGGAACTTC+TGG	+	tig0007163:781-800	Msa1345440:CDS	45.0%
	TCCAGAAGTTCCTGGAATGT+TGG	-	tig0007163:785-804	None:intergenic	45.0%
!	CATTCCAGGAACTTCTGGAA+AGG	+	tig0007163:786-805	Msa1345440:CDS	45.0%
	TAAGCCTTTCCAGAAGTTCC+TGG	-	tig0007163:793-812	None:intergenic	45.0%
	CTCTCAAAGATGGATCAGCT+AGG	-	tig0007163:891-910	None:intergenic	45.0%
!	TTTCTTTTTGTCACTCGCGG+CGG	+	tig0007163:914-933	Msa1345440:three_prime_UTR	45.0%
!!	GATGAGTTGGTACAAACAGC+TGG	+	tig0007163:991-1010	Msa1345440:three_prime_UTR	45.0%
	AGGTCCGACGTTTACAGCTT+TGG	+	tig0007163:124-143	Msa1345440:intron	50.0%
	GGTCCGACGTTTACAGCTTT+GGG	+	tig0007163:125-144	Msa1345440:intron	50.0%
	GTCCGACGTTTACAGCTTTG+GGG	+	tig0007163:126-145	Msa1345440:five_prime_UTR	50.0%
	CACCCCAAAGCTGTAAACGT+CGG	-	tig0007163:131-150	None:intergenic	50.0%
	GGACACTCTCAATGCAATGC+AGG	+	tig0007163:186-205	Msa1345440:CDS	50.0%
	CTGTCTGTTTCGAGCTTAGC+TGG	+	tig0007163:383-402	Msa1345440:intron	50.0%
!!	CTTCCGGAATTTCTAGCCGA+TGG	-	tig0007163:471-490	None:intergenic	50.0%
	TCTGGCTGCTTGGAACTGAA+TGG	-	tig0007163:837-856	None:intergenic	50.0%
	AGGTCCGACGTTTACAGCTT+TGG	+	tig0007163:124-143	Msa1345440:intron	50.0%
	GGTCCGACGTTTACAGCTTT+GGG	+	tig0007163:125-144	Msa1345440:intron	50.0%
	GTCCGACGTTTACAGCTTTG+GGG	+	tig0007163:126-145	Msa1345440:five_prime_UTR	50.0%
	CACCCCAAAGCTGTAAACGT+CGG	-	tig0007163:131-150	None:intergenic	50.0%
	GGACACTCTCAATGCAATGC+AGG	+	tig0007163:186-205	Msa1345440:CDS	50.0%
	CTGTCTGTTTCGAGCTTAGC+TGG	+	tig0007163:383-402	Msa1345440:intron	50.0%
!!	CTTCCGGAATTTCTAGCCGA+TGG	-	tig0007163:471-490	None:intergenic	50.0%
	TCTGGCTGCTTGGAACTGAA+TGG	-	tig0007163:837-856	None:intergenic	50.0%
	TGGAATGTTGGTCGGAGAGC+CGG	-	tig0007163:773-792	None:intergenic	55.0%
	TTCCAAGCAGCCAGATCTGC+TGG	+	tig0007163:842-861	Msa1345440:CDS	55.0%
	AAGCAGCCAGATCTGCTGGT+GGG	+	tig0007163:846-865	Msa1345440:CDS	55.0%
!	CTTTTCCCCACCAGCAGATC+TGG	-	tig0007163:855-874	None:intergenic	55.0%
	TGGAATGTTGGTCGGAGAGC+CGG	-	tig0007163:773-792	None:intergenic	55.0%
	TTCCAAGCAGCCAGATCTGC+TGG	+	tig0007163:842-861	Msa1345440:CDS	55.0%
	AAGCAGCCAGATCTGCTGGT+GGG	+	tig0007163:846-865	Msa1345440:CDS	55.0%
!	CTTTTCCCCACCAGCAGATC+TGG	-	tig0007163:855-874	None:intergenic	55.0%
	GGCTCTCCGACCAACATTCC+AGG	+	tig0007163:772-791	Msa1345440:CDS	60.0%
	CACCAGCAGATCTGGCTGCT+TGG	-	tig0007163:847-866	None:intergenic	60.0%
	CAAGCAGCCAGATCTGCTGG+TGG	+	tig0007163:845-864	Msa1345440:CDS	60.0%
	AGCAGCCAGATCTGCTGGTG+GGG	+	tig0007163:847-866	Msa1345440:CDS	60.0%
	CTCGCGGCGGCTGATGTATA+AGG	+	tig0007163:927-946	Msa1345440:three_prime_UTR	60.0%
	GGCTCTCCGACCAACATTCC+AGG	+	tig0007163:772-791	Msa1345440:CDS	60.0%
	CACCAGCAGATCTGGCTGCT+TGG	-	tig0007163:847-866	None:intergenic	60.0%
	CAAGCAGCCAGATCTGCTGG+TGG	+	tig0007163:845-864	Msa1345440:CDS	60.0%
	AGCAGCCAGATCTGCTGGTG+GGG	+	tig0007163:847-866	Msa1345440:CDS	60.0%
	CTCGCGGCGGCTGATGTATA+AGG	+	tig0007163:927-946	Msa1345440:three_prime_UTR	60.0%

Chromosome	Type	Strat	End	Strand	Name
tig0007163	gene	1	1298	1	ID=Msa1345440;Name=Msa1345440
tig0007163	mRNA	1	1298	1	ID=Msa1345440-mRNA-1;Parent=Msa1345440;Name=Msa1345440-mRNA-1;_AED=0.12;_eAED=0.12;_QI=53\|1\|1\|1\|0.66\|0.75\|4\|406\|91
tig0007163	exon	1	25	1	ID=Msa1345440-mRNA-1:exon:4993;Parent=Msa1345440-mRNA-1
tig0007163	exon	126	207	126	ID=Msa1345440-mRNA-1:exon:4994;Parent=Msa1345440-mRNA-1
tig0007163	exon	442	536	442	ID=Msa1345440-mRNA-1:exon:4995;Parent=Msa1345440-mRNA-1
tig0007163	exon	766	1298	766	ID=Msa1345440-mRNA-1:exon:4996;Parent=Msa1345440-mRNA-1
tig0007163	five_prime_UTR	1	25	1	ID=Msa1345440-mRNA-1:five_prime_utr;Parent=Msa1345440-mRNA-1
tig0007163	five_prime_UTR	126	153	126	ID=Msa1345440-mRNA-1:five_prime_utr;Parent=Msa1345440-mRNA-1
tig0007163	CDS	154	207	154	ID=Msa1345440-mRNA-1:cds;Parent=Msa1345440-mRNA-1
tig0007163	CDS	442	536	442	ID=Msa1345440-mRNA-1:cds;Parent=Msa1345440-mRNA-1
tig0007163	CDS	766	892	766	ID=Msa1345440-mRNA-1:cds;Parent=Msa1345440-mRNA-1
tig0007163	three_prime_UTR	893	1298	893	ID=Msa1345440-mRNA-1:three_prime_utr;Parent=Msa1345440-mRNA-1

Gene Sequence	>Msa1345440 ATGTGGGAACTTGCAACAGAAAAAATCCCTTGGGACACTCTCAATGCAATGCAGGTTATTGGAGCTGTAGGATTTATGAACCATCGGCTAGAAATTCCGGAAGATATTGATCCACAGTGGGCTTCTATAATCGAGAGTTGTTGGCACACTGATCCGGCTCTCCGACCAACATTCCAGGAACTTCTGGAAAGGCTTAAAGAACTTCAGAGACGGTATGCCATTCAGTTCCAAGCAGCCAGATCTGCTGGTGGGGAAAAGACTCAAAAAGAGTCCTAG
Protein sequence	>Msa1345440 MWELATEKIPWDTLNAMQVIGAVGFMNHRLEIPEDIDPQWASIIESCWHTDPALRPTFQELLERLKELQRRYAIQFQAARSAGGEKTQKES*