AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1347810

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1347810 MtrunA17_Chr4g0038511 98.582 282 4 0 1 282 1 282 0.0 568
Msa1347810 MtrunA17_Chr1g0161461 53.597 278 111 5 6 272 77 347 5.25e-91 273
Msa1347810 MtrunA17_Chr4g0042041 44.361 133 57 3 6 137 76 192 6.97e-25 99.8
Msa1347810 MtrunA17_Chr2g0319301 52.083 96 45 1 9 103 72 167 2.33e-23 95.5
Msa1347810 MtrunA17_Chr4g0060591 45.000 100 55 0 7 106 29 128 4.59e-21 89.0
Msa1347810 MtrunA17_Chr1g0167821 40.000 140 71 4 11 144 95 227 9.97e-19 83.2
Msa1347810 MtrunA17_Chr2g0323031 45.263 95 51 1 11 104 110 204 2.05e-18 82.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1347810 AT3G19860.1 62.348 247 79 5 6 246 5 243 7.52e-95 281
Msa1347810 AT3G19860.3 62.348 247 79 5 6 246 36 274 1.47e-94 281
Msa1347810 AT3G19860.2 61.943 247 80 5 6 246 58 296 4.17e-94 281
Msa1347810 AT4G36060.2 44.364 275 103 8 9 274 29 262 7.00e-54 176
Msa1347810 AT4G36060.1 43.636 275 105 7 9 274 47 280 3.48e-53 175
Msa1347810 AT4G36060.4 45.106 235 87 6 9 235 29 229 1.16e-51 170
Msa1347810 AT4G36060.3 45.106 235 87 6 9 235 47 247 5.78e-51 169
Msa1347810 AT5G54680.1 37.975 158 70 4 11 167 76 206 7.04e-22 92.0
Msa1347810 AT4G14410.2 40.152 132 64 4 11 132 129 255 1.60e-18 83.6
Msa1347810 AT4G14410.3 40.152 132 64 4 11 132 129 255 1.60e-18 83.6
Msa1347810 AT4G14410.1 40.152 132 64 4 11 132 135 261 2.76e-18 83.2
Msa1347810 AT1G51070.1 44.681 94 51 1 11 103 71 164 6.51e-18 80.9
Msa1347810 AT3G23210.2 35.606 132 77 2 11 134 138 269 7.97e-18 82.0
Msa1347810 AT1G51070.2 44.681 94 51 1 11 103 137 230 1.46e-17 81.3
Msa1347810 AT3G23210.1 35.606 132 77 2 11 134 167 298 1.75e-17 81.3
Msa1347810 AT3G47640.1 37.113 97 61 0 7 103 28 124 8.81e-16 75.5
Msa1347810 AT3G47640.3 37.113 97 61 0 7 103 28 124 8.81e-16 75.5
Msa1347810 AT3G47640.2 37.113 97 61 0 7 103 28 124 8.81e-16 75.5

Find 97 sgRNAs with CRISPR-Local

Find 169 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTTAGGAGAGTTTGTGATTA+AGG 0.196287 tig0010115:-18773 None:intergenic
AATGATAAAGCGACCATTCT+AGG 0.234923 tig0010115:+17536 Msa1347810:CDS
AATTGTATTCGGAGCTAAAA+AGG 0.247255 tig0010115:-17998 None:intergenic
TATTTCATCTAGTCTTTGTT+TGG 0.256862 tig0010115:+18807 Msa1347810:three_prime_UTR
CGAACTACCTTCTGTGAAAC+TGG 0.280327 tig0010115:-18347 None:intergenic
TAATCACAAACTCTCCTAAA+AGG 0.301913 tig0010115:+18776 Msa1347810:three_prime_UTR
TCCTTAGTTGCAGCTGATAC+TGG 0.331417 tig0010115:-17807 None:intergenic
TTCAATTTGTCACAAGGAAT+AGG 0.340147 tig0010115:-18691 None:intergenic
TGTTGTTCAACAATTGTATT+CGG 0.342434 tig0010115:-18009 None:intergenic
TTCTTCATCTCACAGTGTTC+AGG 0.343603 tig0010115:+18381 Msa1347810:CDS
GGTAGCCGGTCCCACGTATC+AGG 0.344490 tig0010115:+18066 Msa1347810:CDS
ACCAGTATCAGCTGCAACTA+AGG 0.363603 tig0010115:+17806 Msa1347810:CDS
TAGTGGAAGAGTTGTCGTTT+TGG 0.365267 tig0010115:-16426 None:intergenic
TAGCAGGTGTAATTTCCATT+GGG 0.369888 tig0010115:-17157 None:intergenic
AACAAGAACAAGAATAAGAT+TGG 0.370287 tig0010115:+16505 Msa1347810:five_prime_UTR
TTAGGAGAGTTTGTGATTAA+GGG 0.370502 tig0010115:-18772 None:intergenic
GATACGTGGGACCGGCTACC+TGG 0.378928 tig0010115:-18063 None:intergenic
ACATATGTTGAACAAGGGTT+AGG 0.393166 tig0010115:-17973 None:intergenic
TCTGAAATGCAGACCCTGAT+AGG 0.394717 tig0010115:+17507 Msa1347810:intron
AGGATGTTGATTAGCATAGT+AGG 0.395188 tig0010115:-17941 None:intergenic
GCAGGTACCGGCATTGGAAC+TGG 0.401184 tig0010115:-17892 None:intergenic
TCCTGGGTCTTCCGCAGATC+AGG 0.409087 tig0010115:+18182 Msa1347810:CDS
CTAGCAGGTGTAATTTCCAT+TGG 0.415608 tig0010115:-17158 None:intergenic
GACCTTGAGTTGAAGACTCC+TGG 0.425984 tig0010115:+18165 Msa1347810:CDS
GTTTACTAACTTGAGAAGTA+AGG 0.433635 tig0010115:-17583 None:intergenic
TTCTTTGAGTTGTGAGGGAA+AGG 0.439952 tig0010115:+18642 Msa1347810:three_prime_UTR
TCTGTTCTCTCTTCTTAACT+AGG 0.442611 tig0010115:+18579 Msa1347810:three_prime_UTR
GTTCAATTTCCAGTTGTCTC+AGG 0.452373 tig0010115:+17723 Msa1347810:intron
CGCCTCTTCATCCAGGTAGC+CGG 0.455426 tig0010115:+18052 Msa1347810:CDS
TCTTTGAGCCTTGCTCTCCA+TGG 0.456467 tig0010115:-18115 None:intergenic
ATAACGGAATGATCCATTGC+AGG 0.461908 tig0010115:-17844 None:intergenic
GGCTACCTGGATGAAGAGGC+GGG 0.476225 tig0010115:-18050 None:intergenic
TTCACAGAAGGTAGTTCGTT+AGG 0.482876 tig0010115:+18352 Msa1347810:CDS
GAACCAGCAGGTACCGGCAT+TGG 0.486966 tig0010115:-17898 None:intergenic
AGGCTGACCGAGAAAAGTTA+AGG 0.489877 tig0010115:+17192 Msa1347810:CDS
GTTCCAATGCCGGTACCTGC+TGG 0.492349 tig0010115:+17895 Msa1347810:CDS
GGATGTTGATTAGCATAGTA+GGG 0.493252 tig0010115:-17940 None:intergenic
GGTGAAAAGTGAAAGGATAG+TGG 0.493351 tig0010115:-16443 None:intergenic
CATATGTTGAACAAGGGTTA+GGG 0.508234 tig0010115:-17972 None:intergenic
ACCGGCATTGGAACTGGATA+CGG 0.508537 tig0010115:-17886 None:intergenic
ACCTTGAGTTGAAGACTCCT+GGG 0.508601 tig0010115:+18166 Msa1347810:CDS
CAAATCATCCATGGAGAGCA+AGG 0.508721 tig0010115:+18107 Msa1347810:CDS
ACAAGAACAAGAATAAGATT+GGG 0.509441 tig0010115:+16506 Msa1347810:five_prime_UTR
GAAAATGTTGAACGTACGTC+TGG 0.514766 tig0010115:+18839 Msa1347810:three_prime_UTR
TCCGTATCCAGTTCCAATGC+CGG 0.524495 tig0010115:+17885 Msa1347810:CDS
AGACCAGTGTGGCTAAATGT+AGG 0.525327 tig0010115:+18740 Msa1347810:three_prime_UTR
GAATGATCCATTGCAGGCCA+TGG 0.527462 tig0010115:-17838 None:intergenic
ACCCTCACAGTAGCTTCAAA+AGG 0.527695 tig0010115:-18482 None:intergenic
TGACAAATTGAAATTGCAGA+TGG 0.531031 tig0010115:+18702 Msa1347810:three_prime_UTR
GAAAGGCTAGTGAATGAGAA+TGG 0.532624 tig0010115:+18434 Msa1347810:exon
TGCAATTTCAATTTGTCACA+AGG 0.534577 tig0010115:-18697 None:intergenic
ATCTAGTAAGTCGTCGAGGA+AGG 0.538754 tig0010115:+18321 Msa1347810:CDS
TTGAACTGTATCTCCTAGAA+TGG 0.541064 tig0010115:-17549 None:intergenic
AATGGATCATTCCGTTATGA+TGG 0.543467 tig0010115:+17849 Msa1347810:CDS
GGAATTGAACCAGCAGGTAC+CGG 0.545624 tig0010115:-17904 None:intergenic
ACCTGCTAGAAAGACACAAA+AGG 0.554651 tig0010115:+17172 Msa1347810:CDS
GTATCCCCGCCTCTTCATCC+AGG 0.556309 tig0010115:+18045 Msa1347810:CDS
TATGTATCCATGGCCTGCAA+TGG 0.558315 tig0010115:+17831 Msa1347810:CDS
TAGTAGGGGTACGGCTGCAT+GGG 0.560037 tig0010115:-17925 None:intergenic
AGCAGGTGTAATTTCCATTG+GGG 0.561289 tig0010115:-17156 None:intergenic
ATAGTAGGGGTACGGCTGCA+TGG 0.562866 tig0010115:-17926 None:intergenic
AACTAAGGACTATGTATCCA+TGG 0.564461 tig0010115:+17821 Msa1347810:CDS
CGGCTACCTGGATGAAGAGG+CGG 0.573692 tig0010115:-18051 None:intergenic
TAGTGTAGTGGTGAGCAGAA+AGG 0.576515 tig0010115:+18417 Msa1347810:CDS
GCATCAACCATAGATAGATA+AGG 0.576735 tig0010115:+16552 Msa1347810:five_prime_UTR
ACCCAGGAGTCTTCAACTCA+AGG 0.576772 tig0010115:-18167 None:intergenic
GGTCTGAAGTTGAACCCCAA+TGG 0.587215 tig0010115:+17142 Msa1347810:intron
TGATTAGCATAGTAGGGGTA+CGG 0.592321 tig0010115:-17934 None:intergenic
AAACCTACATTTAGCCACAC+TGG 0.600095 tig0010115:-18743 None:intergenic
TTAAGAAGAGAGAACAGAAG+CGG 0.601746 tig0010115:-18574 None:intergenic
AAGGGAACATATGTTGAACA+AGG 0.603401 tig0010115:-17979 None:intergenic
GTTGAACGTACGTCTGGACT+CGG 0.603755 tig0010115:+18845 Msa1347810:three_prime_UTR
GACCGGCTACCTGGATGAAG+AGG 0.604106 tig0010115:-18054 None:intergenic
TTCTGCTGCAACTATTGTAG+AGG 0.604657 tig0010115:+18516 Msa1347810:three_prime_UTR
GGAACTGGATACGGATATGT+TGG 0.606135 tig0010115:-17877 None:intergenic
AAGGAAACCAGTTTCACAGA+AGG 0.607572 tig0010115:+18340 Msa1347810:CDS
GAGCAAGGCTCAAAGAAATG+AGG 0.610042 tig0010115:+18122 Msa1347810:CDS
ACCTGATCTGCGGAAGACCC+AGG 0.614780 tig0010115:-18183 None:intergenic
AGATACAGTTCAATTGCTGA+AGG 0.616836 tig0010115:+17559 Msa1347810:CDS
TGTTGGTGGAGCCATCATAA+CGG 0.621484 tig0010115:-17860 None:intergenic
CAAGGGTTAGGGATGACTGC+AGG 0.622814 tig0010115:-17961 None:intergenic
AGGCGGGGATACGTATTGAG+TGG 0.624785 tig0010115:-18034 None:intergenic
AAGACTAGATGAAATAAGCA+TGG 0.625916 tig0010115:-18800 None:intergenic
TAGCTCATCAAGTAGTGTAG+TGG 0.626211 tig0010115:+18405 Msa1347810:CDS
CTGACCGAGAAAAGTTAAGG+AGG 0.634700 tig0010115:+17195 Msa1347810:CDS
AGCAGCACTAACCTGATCTG+CGG 0.637188 tig0010115:-18193 None:intergenic
TGCATGGGAATTGAACCAGC+AGG 0.642923 tig0010115:-17910 None:intergenic
GGATCACAGCAAGCAAACGA+CGG 0.646485 tig0010115:-16405 None:intergenic
TGACCGAGAAAAGTTAAGGA+GGG 0.651662 tig0010115:+17196 Msa1347810:CDS
GCTAAATGAAGAATCTCGTG+AGG 0.654605 tig0010115:+17625 Msa1347810:CDS
TGTGAGGGAAAGGCTGCGTG+AGG 0.655698 tig0010115:+18652 Msa1347810:three_prime_UTR
ACTGGATACGGATATGTTGG+TGG 0.657323 tig0010115:-17874 None:intergenic
AGTCTTCAACTCAAGGTCTG+TGG 0.666361 tig0010115:-18160 None:intergenic
GATGTTGATTAGCATAGTAG+GGG 0.671724 tig0010115:-17939 None:intergenic
GCTACCTGGATGAAGAGGCG+GGG 0.673729 tig0010115:-18049 None:intergenic
AGGGAACATATGTTGAACAA+GGG 0.680887 tig0010115:-17978 None:intergenic
TCTTCAAGTTGAGACCAGTG+TGG 0.762333 tig0010115:+18729 Msa1347810:three_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTTAATTATTTTGTGTTTT+TGG + tig0010115:17097-17116 Msa1347810:intron 10.0%
!!! AAAAAGCTCAAATTTTTAAA+AGG - tig0010115:16685-16704 None:intergenic 15.0%
!!! TATAAATTGATTCAAATCAA+TGG + tig0010115:16851-16870 Msa1347810:intron 15.0%
!!! TGAATTGTAATTGTGTTTTT+CGG - tig0010115:16474-16493 None:intergenic 20.0%
!!! ATCTTCTTCAATTTCAATTT+TGG + tig0010115:16579-16598 Msa1347810:intron 20.0%
!! TGTCAATTAAACAATAAAGA+TGG - tig0010115:16647-16666 None:intergenic 20.0%
!! TCTTTATTGTTTAATTGACA+TGG + tig0010115:16647-16666 Msa1347810:intron 20.0%
!! CTTTATTGTTTAATTGACAT+GGG + tig0010115:16648-16667 Msa1347810:intron 20.0%
!! CATTGAATCAAAGTAATTTA+GGG - tig0010115:16729-16748 None:intergenic 20.0%
!! ACATTGAATCAAAGTAATTT+AGG - tig0010115:16730-16749 None:intergenic 20.0%
!! ATTAGATTTCTTAGAAGTTA+GGG + tig0010115:16913-16932 Msa1347810:intron 20.0%
!!! CTTTCTTTTTTTGATCATTT+TGG + tig0010115:17035-17054 Msa1347810:intron 20.0%
!!! ATTTTGGTTGTTATTATTGA+AGG + tig0010115:17051-17070 Msa1347810:intron 20.0%
!!! TAATTGTGTTTTTCGGAAAA+AGG - tig0010115:16467-16486 None:intergenic 25.0%
! AACAAGAACAAGAATAAGAT+TGG + tig0010115:16505-16524 Msa1347810:five_prime_UTR 25.0%
! ACAAGAACAAGAATAAGATT+GGG + tig0010115:16506-16525 Msa1347810:five_prime_UTR 25.0%
!! GATTTTACCTTATCTATCTA+TGG - tig0010115:16562-16581 None:intergenic 25.0%
!!! ATGTACGAGTTTTGTATAAT+TGG + tig0010115:16763-16782 Msa1347810:intron 25.0%
! ATAATTGGTTAATCCCAAAT+TGG + tig0010115:16778-16797 Msa1347810:intron 25.0%
! AAACTCATACAATTCTCAAT+AGG - tig0010115:16884-16903 None:intergenic 25.0%
!! CTAACTTCTAAGAAATCTAA+TGG - tig0010115:16914-16933 None:intergenic 25.0%
! CATTAGATTTCTTAGAAGTT+AGG + tig0010115:16912-16931 Msa1347810:intron 25.0%
! TGTTGTTCAACAATTGTATT+CGG - tig0010115:18012-18031 None:intergenic 25.0%
!!! ATTAAAGCTGTTTTTGTTGT+CGG + tig0010115:18266-18285 Msa1347810:intron 25.0%
!!! CTTACTAGATTTTTTTTGTG+CGG - tig0010115:18311-18330 None:intergenic 25.0%
!! TATTTCATCTAGTCTTTGTT+TGG + tig0010115:18807-18826 Msa1347810:three_prime_UTR 25.0%
! CTTAGAAGTTAGGGTATTAT+TGG + tig0010115:16922-16941 Msa1347810:intron 30.0%
!! TTATTGGCTTCATAATGTGT+AGG + tig0010115:16938-16957 Msa1347810:intron 30.0%
!!! AATGAACAGTTTTTAGAGCT+GGG + tig0010115:17227-17246 Msa1347810:CDS 30.0%
CTAGAATTCATTCATGTGTT+AGG + tig0010115:17414-17433 Msa1347810:intron 30.0%
!! TATCATTTTTAGGCCTATCA+GGG - tig0010115:17523-17542 None:intergenic 30.0%
!! TTATCATTTTTAGGCCTATC+AGG - tig0010115:17524-17543 None:intergenic 30.0%
!! ATGGTCGCTTTATCATTTTT+AGG - tig0010115:17533-17552 None:intergenic 30.0%
GTTTACTAACTTGAGAAGTA+AGG - tig0010115:17586-17605 None:intergenic 30.0%
ATTGTATTCGGAGCTAAAAA+GGG - tig0010115:18000-18019 None:intergenic 30.0%
AATTGTATTCGGAGCTAAAA+AGG - tig0010115:18001-18020 None:intergenic 30.0%
!!! TTTTTTGGACGAGAATAGAA+GGG - tig0010115:18549-18568 None:intergenic 30.0%
!!! AAACTTTTCTTTGAGTTGTG+AGG + tig0010115:18636-18655 Msa1347810:three_prime_UTR 30.0%
!!! AACTTTTCTTTGAGTTGTGA+GGG + tig0010115:18637-18656 Msa1347810:three_prime_UTR 30.0%
TTCAATTTGTCACAAGGAAT+AGG - tig0010115:18694-18713 None:intergenic 30.0%
TGCAATTTCAATTTGTCACA+AGG - tig0010115:18700-18719 None:intergenic 30.0%
TGACAAATTGAAATTGCAGA+TGG + tig0010115:18702-18721 Msa1347810:three_prime_UTR 30.0%
TTAGGAGAGTTTGTGATTAA+GGG - tig0010115:18775-18794 None:intergenic 30.0%
TTTAGGAGAGTTTGTGATTA+AGG - tig0010115:18776-18795 None:intergenic 30.0%
TAATCACAAACTCTCCTAAA+AGG + tig0010115:18776-18795 Msa1347810:three_prime_UTR 30.0%
AAGACTAGATGAAATAAGCA+TGG - tig0010115:18803-18822 None:intergenic 30.0%
GGAAAAAGGTGAAAAGTGAA+AGG - tig0010115:16453-16472 None:intergenic 35.0%
GCATCAACCATAGATAGATA+AGG + tig0010115:16552-16571 Msa1347810:five_prime_UTR 35.0%
!! AGGAACAAATTTTCAAGCAG+TGG + tig0010115:16958-16977 Msa1347810:intron 35.0%
!! AATTTTCAAGCAGTGGCTTT+TGG + tig0010115:16965-16984 Msa1347810:intron 35.0%
! TTTTATGCGTTTACTGCGAA+AGG + tig0010115:17121-17140 Msa1347810:intron 35.0%
TAGCAGGTGTAATTTCCATT+GGG - tig0010115:17160-17179 None:intergenic 35.0%
!!! CAATGAACAGTTTTTAGAGC+TGG + tig0010115:17226-17245 Msa1347810:CDS 35.0%
! TTAGAGCTGGGTAACATTTT+AGG + tig0010115:17239-17258 Msa1347810:CDS 35.0%
! TTTTCAACTACATGTTGCGA+AGG + tig0010115:17342-17361 Msa1347810:intron 35.0%
AATGATAAAGCGACCATTCT+AGG + tig0010115:17536-17555 Msa1347810:CDS 35.0%
TTGAACTGTATCTCCTAGAA+TGG - tig0010115:17552-17571 None:intergenic 35.0%
AGATACAGTTCAATTGCTGA+AGG + tig0010115:17559-17578 Msa1347810:CDS 35.0%
! CGTGAGGTAATATTTTCTCT+CGG + tig0010115:17641-17660 Msa1347810:intron 35.0%
! TCTGGGACATAAATTTACAG+AGG + tig0010115:17686-17705 Msa1347810:intron 35.0%
AACTAAGGACTATGTATCCA+TGG + tig0010115:17821-17840 Msa1347810:CDS 35.0%
AATGGATCATTCCGTTATGA+TGG + tig0010115:17849-17868 Msa1347810:CDS 35.0%
! GATGTTGATTAGCATAGTAG+GGG - tig0010115:17942-17961 None:intergenic 35.0%
! GGATGTTGATTAGCATAGTA+GGG - tig0010115:17943-17962 None:intergenic 35.0%
! AGGATGTTGATTAGCATAGT+AGG - tig0010115:17944-17963 None:intergenic 35.0%
CATATGTTGAACAAGGGTTA+GGG - tig0010115:17975-17994 None:intergenic 35.0%
ACATATGTTGAACAAGGGTT+AGG - tig0010115:17976-17995 None:intergenic 35.0%
AGGGAACATATGTTGAACAA+GGG - tig0010115:17981-18000 None:intergenic 35.0%
AAGGGAACATATGTTGAACA+AGG - tig0010115:17982-18001 None:intergenic 35.0%
CTCCAAAAACAAATCATCCA+TGG + tig0010115:18098-18117 Msa1347810:CDS 35.0%
!!! CTCCATGGATGATTTGTTTT+TGG - tig0010115:18103-18122 None:intergenic 35.0%
!!! GTTAGTGCTGCTTTTTTCTT+GGG + tig0010115:18204-18223 Msa1347810:intron 35.0%
!! TTTTTGTTGTCGGATTTTGC+AGG + tig0010115:18276-18295 Msa1347810:intron 35.0%
AAAAATCTAGTAAGTCGTCG+AGG + tig0010115:18317-18336 Msa1347810:CDS 35.0%
!!! CTTTTTTGGACGAGAATAGA+AGG - tig0010115:18550-18569 None:intergenic 35.0%
TTAAGAAGAGAGAACAGAAG+CGG - tig0010115:18577-18596 None:intergenic 35.0%
TCTGTTCTCTCTTCTTAACT+AGG + tig0010115:18579-18598 Msa1347810:three_prime_UTR 35.0%
! GAAATAAGCATGGTCCTTTT+AGG - tig0010115:18793-18812 None:intergenic 35.0%
!! TAGTGGAAGAGTTGTCGTTT+TGG - tig0010115:16429-16448 None:intergenic 40.0%
GGTGAAAAGTGAAAGGATAG+TGG - tig0010115:16446-16465 None:intergenic 40.0%
GAACTACACAGCACCAATTT+GGG - tig0010115:16794-16813 None:intergenic 40.0%
TGAACTACACAGCACCAATT+TGG - tig0010115:16795-16814 None:intergenic 40.0%
AGCAGGTGTAATTTCCATTG+GGG - tig0010115:17159-17178 None:intergenic 40.0%
CTAGCAGGTGTAATTTCCAT+TGG - tig0010115:17161-17180 None:intergenic 40.0%
ACCTGCTAGAAAGACACAAA+AGG + tig0010115:17172-17191 Msa1347810:CDS 40.0%
TGACCGAGAAAAGTTAAGGA+GGG + tig0010115:17196-17215 Msa1347810:CDS 40.0%
! GATCCCTCCTTAACTTTTCT+CGG - tig0010115:17202-17221 None:intergenic 40.0%
AGAGCACAGAGTAATTCAAG+AGG - tig0010115:17479-17498 None:intergenic 40.0%
TTGAATTACTCTGTGCTCTG+TGG + tig0010115:17480-17499 Msa1347810:intron 40.0%
GCTAAATGAAGAATCTCGTG+AGG + tig0010115:17625-17644 Msa1347810:CDS 40.0%
ATGAATCACCTTGATGCTTC+TGG + tig0010115:17668-17687 Msa1347810:intron 40.0%
TGAATCACCTTGATGCTTCT+GGG + tig0010115:17669-17688 Msa1347810:intron 40.0%
!! TTTATGTCCCAGAAGCATCA+AGG - tig0010115:17679-17698 None:intergenic 40.0%
GTTCAATTTCCAGTTGTCTC+AGG + tig0010115:17723-17742 Msa1347810:intron 40.0%
! CATTTTTCTCCTGAGACAAC+TGG - tig0010115:17735-17754 None:intergenic 40.0%
! ATAACGGAATGATCCATTGC+AGG - tig0010115:17847-17866 None:intergenic 40.0%
TGATTAGCATAGTAGGGGTA+CGG - tig0010115:17937-17956 None:intergenic 40.0%
!!! GTCTTGTTTTCCTGATACGT+GGG - tig0010115:18079-18098 None:intergenic 40.0%
!!! AGTCTTGTTTTCCTGATACG+TGG - tig0010115:18080-18099 None:intergenic 40.0%
!!! GGTTAGTGCTGCTTTTTTCT+TGG + tig0010115:18203-18222 Msa1347810:intron 40.0%
ACCTATAGATGCAGTAGAAG+AGG + tig0010115:18237-18256 Msa1347810:intron 40.0%
ACCTCTTCTACTGCATCTAT+AGG - tig0010115:18241-18260 None:intergenic 40.0%
AAGGAAACCAGTTTCACAGA+AGG + tig0010115:18340-18359 Msa1347810:CDS 40.0%
TTCACAGAAGGTAGTTCGTT+AGG + tig0010115:18352-18371 Msa1347810:CDS 40.0%
TTCTTCATCTCACAGTGTTC+AGG + tig0010115:18381-18400 Msa1347810:CDS 40.0%
TAGCTCATCAAGTAGTGTAG+TGG + tig0010115:18405-18424 Msa1347810:CDS 40.0%
GAAAGGCTAGTGAATGAGAA+TGG + tig0010115:18434-18453 Msa1347810:exon 40.0%
! CTACTGTGAGGGTTTTCATT+GGG + tig0010115:18492-18511 Msa1347810:three_prime_UTR 40.0%
TTCTGCTGCAACTATTGTAG+AGG + tig0010115:18516-18535 Msa1347810:three_prime_UTR 40.0%
!!! ACAGAAGCGGCTTTCTTTTT+TGG - tig0010115:18564-18583 None:intergenic 40.0%
!! TTCTTTGAGTTGTGAGGGAA+AGG + tig0010115:18642-18661 Msa1347810:three_prime_UTR 40.0%
AAACCTACATTTAGCCACAC+TGG - tig0010115:18746-18765 None:intergenic 40.0%
GAAAATGTTGAACGTACGTC+TGG + tig0010115:18839-18858 Msa1347810:three_prime_UTR 40.0%
! GTGGCTTTTGGACATGTCAA+AGG + tig0010115:16977-16996 Msa1347810:intron 45.0%
!! GCCTTTTGTGTCTTTCTAGC+AGG - tig0010115:17176-17195 None:intergenic 45.0%
AGGCTGACCGAGAAAAGTTA+AGG + tig0010115:17192-17211 Msa1347810:CDS 45.0%
CTGACCGAGAAAAGTTAAGG+AGG + tig0010115:17195-17214 Msa1347810:CDS 45.0%
CAAAATGTGAGCAGAGTCAC+TGG + tig0010115:17297-17316 Msa1347810:intron 45.0%
! TCTGAAATGCAGACCCTGAT+AGG + tig0010115:17507-17526 Msa1347810:intron 45.0%
ACCAGTATCAGCTGCAACTA+AGG + tig0010115:17806-17825 Msa1347810:CDS 45.0%
TCCTTAGTTGCAGCTGATAC+TGG - tig0010115:17810-17829 None:intergenic 45.0%
TATGTATCCATGGCCTGCAA+TGG + tig0010115:17831-17850 Msa1347810:CDS 45.0%
! TGTTGGTGGAGCCATCATAA+CGG - tig0010115:17863-17882 None:intergenic 45.0%
ACTGGATACGGATATGTTGG+TGG - tig0010115:17877-17896 None:intergenic 45.0%
GGAACTGGATACGGATATGT+TGG - tig0010115:17880-17899 None:intergenic 45.0%
CAAATCATCCATGGAGAGCA+AGG + tig0010115:18107-18126 Msa1347810:CDS 45.0%
GAGCAAGGCTCAAAGAAATG+AGG + tig0010115:18122-18141 Msa1347810:CDS 45.0%
AGTCTTCAACTCAAGGTCTG+TGG - tig0010115:18163-18182 None:intergenic 45.0%
!! ACCTTGAGTTGAAGACTCCT+GGG + tig0010115:18166-18185 Msa1347810:CDS 45.0%
ATCTAGTAAGTCGTCGAGGA+AGG + tig0010115:18321-18340 Msa1347810:CDS 45.0%
CGAACTACCTTCTGTGAAAC+TGG - tig0010115:18350-18369 None:intergenic 45.0%
TAGTGTAGTGGTGAGCAGAA+AGG + tig0010115:18417-18436 Msa1347810:CDS 45.0%
!!! TGCCTTTTGAAGCTACTGTG+AGG + tig0010115:18480-18499 Msa1347810:three_prime_UTR 45.0%
!!! GCCTTTTGAAGCTACTGTGA+GGG + tig0010115:18481-18500 Msa1347810:three_prime_UTR 45.0%
ACCCTCACAGTAGCTTCAAA+AGG - tig0010115:18485-18504 None:intergenic 45.0%
! GCTACTGTGAGGGTTTTCAT+TGG + tig0010115:18491-18510 Msa1347810:three_prime_UTR 45.0%
!! TCTTCAAGTTGAGACCAGTG+TGG + tig0010115:18729-18748 Msa1347810:three_prime_UTR 45.0%
AGACCAGTGTGGCTAAATGT+AGG + tig0010115:18740-18759 Msa1347810:three_prime_UTR 45.0%
GGATCACAGCAAGCAAACGA+CGG - tig0010115:16408-16427 None:intergenic 50.0%
! GGTCTGAAGTTGAACCCCAA+TGG + tig0010115:17142-17161 Msa1347810:intron 50.0%
GAATGATCCATTGCAGGCCA+TGG - tig0010115:17841-17860 None:intergenic 50.0%
TCCGTATCCAGTTCCAATGC+CGG + tig0010115:17885-17904 Msa1347810:CDS 50.0%
! ACCGGCATTGGAACTGGATA+CGG - tig0010115:17889-17908 None:intergenic 50.0%
GGAATTGAACCAGCAGGTAC+CGG - tig0010115:17907-17926 None:intergenic 50.0%
TGCATGGGAATTGAACCAGC+AGG - tig0010115:17913-17932 None:intergenic 50.0%
! GTTTTCCTGATACGTGGGAC+CGG - tig0010115:18074-18093 None:intergenic 50.0%
TCTTTGAGCCTTGCTCTCCA+TGG - tig0010115:18118-18137 None:intergenic 50.0%
!! GACCTTGAGTTGAAGACTCC+TGG + tig0010115:18165-18184 Msa1347810:CDS 50.0%
ACCCAGGAGTCTTCAACTCA+AGG - tig0010115:18170-18189 None:intergenic 50.0%
AGCAGCACTAACCTGATCTG+CGG - tig0010115:18196-18215 None:intergenic 50.0%
GTTGAACGTACGTCTGGACT+CGG + tig0010115:18845-18864 Msa1347810:three_prime_UTR 50.0%
! TAGTAGGGGTACGGCTGCAT+GGG - tig0010115:17928-17947 None:intergenic 55.0%
! ATAGTAGGGGTACGGCTGCA+TGG - tig0010115:17929-17948 None:intergenic 55.0%
CAAGGGTTAGGGATGACTGC+AGG - tig0010115:17964-17983 None:intergenic 55.0%
AGGCGGGGATACGTATTGAG+TGG - tig0010115:18037-18056 None:intergenic 55.0%
!! GCAGGTACCGGCATTGGAAC+TGG - tig0010115:17895-17914 None:intergenic 60.0%
GTTCCAATGCCGGTACCTGC+TGG + tig0010115:17895-17914 Msa1347810:CDS 60.0%
!! GAACCAGCAGGTACCGGCAT+TGG - tig0010115:17901-17920 None:intergenic 60.0%
GTATCCCCGCCTCTTCATCC+AGG + tig0010115:18045-18064 Msa1347810:CDS 60.0%
GCTACCTGGATGAAGAGGCG+GGG - tig0010115:18052-18071 None:intergenic 60.0%
GGCTACCTGGATGAAGAGGC+GGG - tig0010115:18053-18072 None:intergenic 60.0%
CGGCTACCTGGATGAAGAGG+CGG - tig0010115:18054-18073 None:intergenic 60.0%
CGCCTCTTCATCCAGGTAGC+CGG + tig0010115:18052-18071 Msa1347810:CDS 60.0%
GACCGGCTACCTGGATGAAG+AGG - tig0010115:18057-18076 None:intergenic 60.0%
TCCTGGGTCTTCCGCAGATC+AGG + tig0010115:18182-18201 Msa1347810:CDS 60.0%
ACCTGATCTGCGGAAGACCC+AGG - tig0010115:18186-18205 None:intergenic 60.0%
TGTGAGGGAAAGGCTGCGTG+AGG + tig0010115:18652-18671 Msa1347810:three_prime_UTR 60.0%
GATACGTGGGACCGGCTACC+TGG - tig0010115:18066-18085 None:intergenic 65.0%
GGTAGCCGGTCCCACGTATC+AGG + tig0010115:18066-18085 Msa1347810:CDS 65.0%
Chromosome Type Strat End Strand Name
tig0010115 gene 16361 18873 16361 ID=Msa1347810;Name=Msa1347810
tig0010115 mRNA 16361 18873 16361 ID=Msa1347810-mRNA-1;Parent=Msa1347810;Name=Msa1347810-mRNA-1;_AED=0.11;_eAED=0.11;_QI=231|1|1|1|0.75|0.6|5|423|282
tig0010115 exon 16361 16573 16361 ID=Msa1347810-mRNA-1:exon:6052;Parent=Msa1347810-mRNA-1
tig0010115 exon 17143 17260 17143 ID=Msa1347810-mRNA-1:exon:6053;Parent=Msa1347810-mRNA-1
tig0010115 exon 17519 17646 17519 ID=Msa1347810-mRNA-1:exon:6054;Parent=Msa1347810-mRNA-1
tig0010115 exon 17736 18203 17736 ID=Msa1347810-mRNA-1:exon:6055;Parent=Msa1347810-mRNA-1
tig0010115 exon 18298 18873 18298 ID=Msa1347810-mRNA-1:exon:6056;Parent=Msa1347810-mRNA-1
tig0010115 five_prime_UTR 16361 16573 16361 ID=Msa1347810-mRNA-1:five_prime_utr;Parent=Msa1347810-mRNA-1
tig0010115 five_prime_UTR 17143 17160 17143 ID=Msa1347810-mRNA-1:five_prime_utr;Parent=Msa1347810-mRNA-1
tig0010115 CDS 17161 17260 17161 ID=Msa1347810-mRNA-1:cds;Parent=Msa1347810-mRNA-1
tig0010115 CDS 17519 17646 17519 ID=Msa1347810-mRNA-1:cds;Parent=Msa1347810-mRNA-1
tig0010115 CDS 17736 18203 17736 ID=Msa1347810-mRNA-1:cds;Parent=Msa1347810-mRNA-1
tig0010115 CDS 18298 18450 18298 ID=Msa1347810-mRNA-1:cds;Parent=Msa1347810-mRNA-1
tig0010115 three_prime_UTR 18451 18873 18451 ID=Msa1347810-mRNA-1:three_prime_utr;Parent=Msa1347810-mRNA-1
Gene Sequence

>Msa1347810

ATGGAAATTACACCTGCTAGAAAGACACAAAAGGCTGACCGAGAAAAGTTAAGGAGGGATCGACTCAATGAACAGTTTTTAGAGCTGGGTAACATTTTAGACCCTGATAGGCCTAAAAATGATAAAGCGACCATTCTAGGAGATACAGTTCAATTGCTGAAGGACCTTACTTCTCAAGTTAGTAAACTAAAAGATGAATATACTATGCTAAATGAAGAATCTCGTGAGTTGTCTCAGGAGAAAAATGATCTCAGAGAAGAAAAAGCTTCTCTTAAATCAGATATTGAGAATTTGAATAACCAGTATCAGCTGCAACTAAGGACTATGTATCCATGGCCTGCAATGGATCATTCCGTTATGATGGCTCCACCAACATATCCGTATCCAGTTCCAATGCCGGTACCTGCTGGTTCAATTCCCATGCAGCCGTACCCCTACTATGCTAATCAACATCCTGCAGTCATCCCTAACCCTTGTTCAACATATGTTCCCTTTTTAGCTCCGAATACAATTGTTGAACAACAATCCACTCAATACGTATCCCCGCCTCTTCATCCAGGTAGCCGGTCCCACGTATCAGGAAAACAAGACTCCAAAAACAAATCATCCATGGAGAGCAAGGCTCAAAGAAATGAGGATTCTAATGATGTAGCCACAGACCTTGAGTTGAAGACTCCTGGGTCTTCCGCAGATCAGGATTTAACATCCGCACAAAAAAAATCTAGTAAGTCGTCGAGGAAGGAAACCAGTTTCACAGAAGGTAGTTCGTTAGGCAAGTGTTCTTCATCTCACAGTGTTCAGGATAGCTCATCAAGTAGTGTAGTGGTGAGCAGAAAGGCTAGTGAATGA
Protein sequence

>Msa1347810

MEITPARKTQKADREKLRRDRLNEQFLELGNILDPDRPKNDKATILGDTVQLLKDLTSQVSKLKDEYTMLNEESRELSQEKNDLREEKASLKSDIENLNNQYQLQLRTMYPWPAMDHSVMMAPPTYPYPVPMPVPAGSIPMQPYPYYANQHPAVIPNPCSTYVPFLAPNTIVEQQSTQYVSPPLHPGSRSHVSGKQDSKNKSSMESKAQRNEDSNDVATDLELKTPGSSADQDLTSAQKKSSKSSRKETSFTEGSSLGKCSSSHSVQDSSSSSVVVSRKASE*