Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1348150 | KEH21040.1 | 97.778 | 45 | 1 | 0 | 1 | 45 | 1 | 45 | 1.79e-23 | 97.1 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1348150 | sp|Q7F9R9|PLI2B_ORYSJ | 64.444 | 45 | 16 | 0 | 1 | 45 | 1 | 45 | 7.04e-16 | 69.7 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1348150 | A0A072U5A9 | 97.778 | 45 | 1 | 0 | 1 | 45 | 1 | 45 | 8.53e-24 | 97.1 |
| Gene ID | Type | Classification |
|---|---|---|
| Msa1348150 | TR | Others |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1348150 | MtrunA17_Chr8g0386501 | 97.778 | 45 | 1 | 0 | 1 | 45 | 1 | 45 | 1.64e-27 | 97.1 |
| Msa1348150 | MtrunA17_Chr3g0113051 | 70.455 | 44 | 13 | 0 | 1 | 44 | 1 | 44 | 1.34e-17 | 71.2 |
| Msa1348150 | MtrunA17_Chr1g0152431 | 63.636 | 44 | 16 | 0 | 1 | 44 | 1 | 44 | 5.36e-15 | 64.3 |
| Msa1348150 | MtrunA17_Chr8g0349391 | 59.091 | 44 | 18 | 0 | 1 | 44 | 1 | 44 | 6.16e-13 | 59.3 |
| Msa1348150 | MtrunA17_Chr4g0059371 | 58.140 | 43 | 18 | 0 | 2 | 44 | 3 | 45 | 7.72e-13 | 58.5 |
| Msa1348150 | MtrunA17_Chr7g0247111 | 59.091 | 44 | 18 | 0 | 1 | 44 | 1 | 44 | 7.75e-13 | 58.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1348150 | AT3G55770.4 | 65.909 | 44 | 15 | 0 | 1 | 44 | 1 | 44 | 5.80e-16 | 66.2 |
| Msa1348150 | AT3G55770.6 | 65.909 | 44 | 15 | 0 | 1 | 44 | 1 | 44 | 6.67e-16 | 65.9 |
| Msa1348150 | AT1G10200.1 | 65.909 | 44 | 15 | 0 | 1 | 44 | 1 | 44 | 1.89e-15 | 65.9 |
| Msa1348150 | AT2G45800.1 | 63.636 | 44 | 16 | 0 | 1 | 44 | 1 | 44 | 2.79e-15 | 66.2 |
| Msa1348150 | AT1G01780.1 | 61.364 | 44 | 17 | 0 | 1 | 44 | 1 | 44 | 8.54e-14 | 62.0 |
| Msa1348150 | AT3G61230.1 | 58.140 | 43 | 18 | 0 | 2 | 44 | 3 | 45 | 8.80e-14 | 62.0 |
| Msa1348150 | AT3G55770.7 | 65.909 | 44 | 15 | 0 | 1 | 44 | 1 | 44 | 1.26e-13 | 61.6 |
| Msa1348150 | AT3G55770.3 | 65.909 | 44 | 15 | 0 | 1 | 44 | 1 | 44 | 1.81e-13 | 60.8 |
| Msa1348150 | AT3G55770.5 | 65.909 | 44 | 15 | 0 | 1 | 44 | 1 | 44 | 1.81e-13 | 60.8 |
| Msa1348150 | AT3G55770.2 | 65.909 | 44 | 15 | 0 | 1 | 44 | 1 | 44 | 1.81e-13 | 60.8 |
| Msa1348150 | AT3G55770.1 | 65.909 | 44 | 15 | 0 | 1 | 44 | 1 | 44 | 1.81e-13 | 60.8 |
| Msa1348150 | AT2G39900.1 | 63.636 | 44 | 16 | 0 | 1 | 44 | 1 | 44 | 5.25e-13 | 59.7 |
Find 10 sgRNAs with CRISPR-Local
Find 6 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CACAAGCCTTGCATTTCTGT+TGG | 0.387121 | tig0010194:-142408 | None:intergenic |
| ACAAGCCTTGCATTTCTGTT+GGG | 0.412168 | tig0010194:-142407 | None:intergenic |
| TCAATAACCATGTCTTTCAC+TGG | 0.477166 | tig0010194:+142382 | None:intergenic |
| GTTGATACATTATCTGCTGA+TGG | 0.560388 | tig0010194:+142448 | Msa1348150:CDS |
| TTGTGATAAAACTGTTCACT+TGG | 0.580761 | tig0010194:+142426 | Msa1348150:CDS |
| TTCAGATGTAATCAATGCAA+TGG | 0.611041 | tig0010194:+142493 | Msa1348150:CDS |
| CGTATGTATATACCGCGAGA+AGG | 0.635292 | tig0010194:-142516 | None:intergenic |
| TGGAACCCAACAGAAATGCA+AGG | 0.666590 | tig0010194:+142402 | Msa1348150:CDS |
| TTGGGTTCCAGTGAAAGACA+TGG | 0.695332 | tig0010194:-142389 | None:intergenic |
| TCAATGCAATGGCCTTCTCG+CGG | 0.702963 | tig0010194:+142504 | Msa1348150:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| TTGTGATAAAACTGTTCACT+TGG | + | tig0010194:142426-142445 | Msa1348150:CDS | 30.0% | |
| TTCAGATGTAATCAATGCAA+TGG | + | tig0010194:142493-142512 | Msa1348150:CDS | 30.0% | |
| GTTGATACATTATCTGCTGA+TGG | + | tig0010194:142448-142467 | Msa1348150:CDS | 35.0% | |
| ACAAGCCTTGCATTTCTGTT+GGG | - | tig0010194:142410-142429 | None:intergenic | 40.0% | |
| TGGAACCCAACAGAAATGCA+AGG | + | tig0010194:142402-142421 | Msa1348150:CDS | 45.0% | |
| CACAAGCCTTGCATTTCTGT+TGG | - | tig0010194:142411-142430 | None:intergenic | 45.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| tig0010194 | gene | 142391 | 142525 | 142391 | ID=Msa1348150;Name=Msa1348150 |
| tig0010194 | mRNA | 142391 | 142525 | 142391 | ID=Msa1348150-mRNA-1;Parent=Msa1348150;Name=Msa1348150-mRNA-1;_AED=0.08;_eAED=0.08;_QI=0|-1|0|1|-1|1|1|0|45 |
| tig0010194 | exon | 142391 | 142525 | 142391 | ID=Msa1348150-mRNA-1:exon:6221;Parent=Msa1348150-mRNA-1 |
| tig0010194 | CDS | 142391 | 142525 | 142391 | ID=Msa1348150-mRNA-1:cds;Parent=Msa1348150-mRNA-1 |
| Gene Sequence |
| Protein sequence |
