AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1349130

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1349130 MtrunA17_Chr1g0175601 95.000 180 8 1 1 179 1 180 4.90e-122 342
Msa1349130 MtrunA17_Chr1g0180591 80.447 179 35 0 1 179 1 179 1.52e-103 295
Msa1349130 MtrunA17_Chr1g0175611 84.524 168 26 0 1 168 1 168 2.15e-101 289
Msa1349130 MtrunA17_Chr1g0169761 78.771 179 38 0 1 179 1 179 2.57e-101 289
Msa1349130 MtrunA17_Chr4g0074101 70.408 196 41 1 1 179 1 196 5.05e-95 274
Msa1349130 MtrunA17_Chr8g0376821 78.916 166 35 0 1 166 1 166 4.88e-92 266
Msa1349130 MtrunA17_Chr8g0357771 58.286 175 73 0 1 175 1 175 7.86e-69 207
Msa1349130 MtrunA17_Chr8g0356071 52.381 168 74 2 1 168 1 162 3.91e-53 166
Msa1349130 MtrunA17_Chr4g0052061 39.634 164 87 3 1 164 1 152 1.14e-30 109
Msa1349130 MtrunA17_Chr3g0099411 42.029 138 77 2 2 136 9 146 1.12e-29 108
Msa1349130 MtrunA17_Chr3g0119901 39.053 169 86 4 1 157 8 171 3.08e-29 107
Msa1349130 MtrunA17_Chr1g0206991 44.444 117 55 2 2 112 9 121 2.91e-26 99.0
Msa1349130 MtrunA17_Chr1g0206721 45.133 113 60 1 2 112 9 121 3.22e-26 100
Msa1349130 MtrunA17_Chr5g0419601 33.533 167 93 3 1 152 8 171 7.06e-26 99.4
Msa1349130 MtrunA17_Chr3g0085241 43.860 114 62 1 1 112 40 153 7.16e-26 99.4
Msa1349130 MtrunA17_Chr1g0206701 45.946 111 57 2 2 110 9 118 1.81e-25 98.2
Msa1349130 MtrunA17_Chr3g0099421 45.045 111 58 2 2 110 34 143 4.88e-25 97.4
Msa1349130 MtrunA17_Chr1g0149391 44.144 111 59 2 2 110 9 118 1.37e-24 95.9
Msa1349130 MtrunA17_Chr2g0300421 40.157 127 73 2 2 126 9 134 2.99e-24 93.2
Msa1349130 MtrunA17_Chr1g0208561 43.564 101 55 1 2 100 9 109 3.77e-24 94.4
Msa1349130 MtrunA17_Chr3g0089141 53.247 77 36 0 2 78 9 85 5.35e-24 94.0
Msa1349130 MtrunA17_Chr2g0292791 54.545 77 35 0 2 78 9 85 6.36e-24 94.0
Msa1349130 MtrunA17_Chr8g0355091 44.144 111 59 2 2 110 9 118 6.64e-24 94.0
Msa1349130 MtrunA17_Chr4g0052081 39.041 146 84 3 1 143 1 144 1.17e-23 92.0
Msa1349130 MtrunA17_Chr2g0325971 43.243 111 60 2 2 110 9 118 1.42e-23 92.8
Msa1349130 MtrunA17_Chr1g0187361 51.948 77 37 0 2 78 9 85 8.01e-23 91.3
Msa1349130 MtrunA17_Chr1g0188671 43.564 101 55 1 2 100 47 147 8.09e-23 92.0
Msa1349130 MtrunA17_Chr1g0208581 42.574 101 56 1 2 100 9 109 9.02e-23 90.9
Msa1349130 MtrunA17_Chr3g0089151 41.818 110 62 1 2 109 9 118 1.78e-22 87.4
Msa1349130 MtrunA17_Chr2g0333981 36.879 141 72 3 1 138 11 137 3.34e-22 88.2
Msa1349130 MtrunA17_Chr2g0300441 44.068 118 61 3 2 117 9 123 4.24e-22 89.4
Msa1349130 MtrunA17_Chr2g0317481 50.685 73 36 0 2 74 9 81 1.03e-21 88.2
Msa1349130 MtrunA17_Chr2g0317451 50.685 73 36 0 2 74 9 81 1.11e-21 88.2
Msa1349130 MtrunA17_Chr5g0422421 53.623 69 32 0 2 70 9 77 1.70e-21 87.8
Msa1349130 MtrunA17_Chr1g0206691 45.361 97 51 1 18 112 8 104 1.58e-20 84.7
Msa1349130 MtrunA17_Chr4g0018951 36.090 133 73 2 14 140 24 150 1.61e-20 83.6
Msa1349130 MtrunA17_Chr5g0422941 48.000 75 39 0 2 76 9 83 2.82e-20 84.3
Msa1349130 MtrunA17_Chr7g0246431 40.385 104 59 1 1 104 1 101 1.26e-19 82.4
Msa1349130 MtrunA17_Chr8g0379021 35.252 139 75 4 2 138 12 137 1.33e-19 81.3
Msa1349130 MtrunA17_Chr8g0380021 43.269 104 56 1 1 104 17 117 2.19e-19 82.4
Msa1349130 MtrunA17_Chr4g0020221 41.509 106 56 1 1 100 1 106 3.86e-19 81.6
Msa1349130 MtrunA17_Chr7g0238991 55.224 67 30 0 13 79 32 98 1.57e-18 80.1
Msa1349130 MtrunA17_Chr3g0097101 42.453 106 58 1 1 106 16 118 1.77e-18 79.7
Msa1349130 MtrunA17_Chr2g0284911 42.308 104 57 1 1 104 16 116 3.01e-18 79.3
Msa1349130 MtrunA17_Chr8g0351041 38.462 104 62 1 1 104 1 102 5.85e-18 76.6
Msa1349130 MtrunA17_Chr5g0427921 56.667 60 26 0 1 60 1 60 6.16e-18 73.9
Msa1349130 MtrunA17_Chr1g0187351 60.377 53 21 0 18 70 8 60 8.18e-18 73.9
Msa1349130 MtrunA17_Chr1g0173161 50.769 65 32 0 1 65 1 65 1.40e-17 73.6
Msa1349130 MtrunA17_Chr2g0279821 52.174 69 33 0 1 69 1 69 2.33e-17 76.6
Msa1349130 MtrunA17_Chr5g0427401 39.823 113 54 4 1 105 1 107 3.73e-17 75.9
Msa1349130 MtrunA17_Chr8g0351031 38.462 104 62 1 1 104 1 102 5.18e-17 75.5
Msa1349130 MtrunA17_Chr8g0351051 31.469 143 90 2 1 143 1 135 5.27e-17 75.9
Msa1349130 MtrunA17_Chr3g0111841 44.928 69 37 1 8 75 2 70 6.98e-17 71.2
Msa1349130 MtrunA17_Chr3g0077501 53.226 62 29 0 1 62 1 62 8.56e-17 75.1
Msa1349130 MtrunA17_Chr5g0412461 34.483 145 77 4 1 140 1 132 1.46e-16 74.3
Msa1349130 MtrunA17_Chr4g0057151 46.479 71 38 0 1 71 1 71 2.85e-16 73.2
Msa1349130 MtrunA17_Chr4g0071601 51.429 70 34 0 1 70 11 80 3.57e-16 72.4
Msa1349130 MtrunA17_Chr4g0049901 41.860 86 49 1 1 86 27 111 4.89e-16 73.2
Msa1349130 MtrunA17_Chr4g0049541 47.826 69 36 0 1 69 1 69 6.46e-16 72.8
Msa1349130 MtrunA17_Chr5g0412271 44.928 69 38 0 1 69 1 69 1.10e-15 72.0
Msa1349130 MtrunA17_Chr5g0411691 40.777 103 55 2 1 103 1 97 1.16e-15 72.0
Msa1349130 MtrunA17_Chr3g0142191 50.000 62 31 0 1 62 1 62 1.43e-15 71.6
Msa1349130 MtrunA17_Chr5g0425191 60.000 50 20 0 1 50 1 50 1.49e-15 68.9
Msa1349130 MtrunA17_Chr5g0404951 48.387 62 32 0 1 62 1 62 2.13e-15 71.2
Msa1349130 MtrunA17_Chr1g0164791 59.184 49 20 0 1 49 1 49 2.49e-15 71.2
Msa1349130 MtrunA17_Chr6g0474371 34.532 139 81 4 1 134 1 134 2.58e-15 70.5
Msa1349130 MtrunA17_Chr1g0204111 46.377 69 37 0 1 69 17 85 2.67e-15 71.6
Msa1349130 MtrunA17_Chr5g0420081 50.877 57 28 0 1 57 1 57 3.54e-15 70.9
Msa1349130 MtrunA17_Chr5g0420091 52.632 57 27 0 1 57 1 57 3.70e-15 70.5
Msa1349130 MtrunA17_Chr4g0044071 55.769 52 23 0 1 52 1 52 4.24e-15 67.8
Msa1349130 MtrunA17_Chr8g0377561 52.632 57 27 0 1 57 1 57 5.71e-15 67.0
Msa1349130 MtrunA17_Chr2g0308251 41.096 73 42 1 1 72 1 73 6.56e-15 66.6
Msa1349130 MtrunA17_Chr7g0220111 52.632 57 27 0 1 57 1 57 6.81e-15 70.1
Msa1349130 MtrunA17_Chr1g0159661 32.407 108 68 2 1 105 1 106 6.83e-15 68.9
Msa1349130 MtrunA17_Chr5g0417661 51.852 54 26 0 1 54 56 109 7.90e-15 67.8
Msa1349130 MtrunA17_Chr3g0111931 47.541 61 32 0 18 78 6 66 9.24e-15 67.0
Msa1349130 MtrunA17_Chr7g0246381 44.928 69 33 1 1 64 1 69 1.14e-14 65.5
Msa1349130 MtrunA17_Chr8g0366231 59.184 49 20 0 1 49 1 49 1.17e-14 69.3
Msa1349130 MtrunA17_Chr3g0122251 54.386 57 26 0 1 57 1 57 1.44e-14 69.3
Msa1349130 MtrunA17_Chr4g0061671 50.000 58 29 0 1 58 1 58 1.45e-14 68.2
Msa1349130 MtrunA17_Chr4g0061691 49.123 57 29 0 1 57 1 57 1.57e-14 68.9
Msa1349130 MtrunA17_Chr3g0125241 41.538 65 38 0 1 65 26 90 1.98e-14 68.2
Msa1349130 MtrunA17_Chr6g0455511 52.632 57 27 0 1 57 1 57 2.61e-14 68.6
Msa1349130 MtrunA17_Chr7g0220121 55.102 49 22 0 1 49 1 49 3.64e-14 68.6
Msa1349130 MtrunA17_Chr3g0134451 41.026 78 45 1 1 77 1 78 5.94e-14 68.6
Msa1349130 MtrunA17_Chr1g0166611 68.182 44 14 0 15 58 19 62 1.32e-13 64.7
Msa1349130 MtrunA17_Chr4g0018421 47.541 61 32 0 1 61 1 61 4.13e-13 64.7
Msa1349130 MtrunA17_Chr4g0060841 39.241 79 46 1 1 77 1 79 1.76e-12 64.3
Msa1349130 MtrunA17_Chr3g0111831 52.083 48 23 0 18 65 20 67 3.31e-12 59.7
Msa1349130 MtrunA17_Chr8g0345001 45.588 68 36 1 3 70 19 85 5.71e-12 61.2
Msa1349130 MtrunA17_Chr3g0093131 49.057 53 26 1 19 70 36 88 6.60e-11 58.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1349130 AT2G34440.1 43.103 174 83 4 1 161 1 171 1.23e-35 122
Msa1349130 AT3G66656.1 74.242 66 17 0 1 66 1 66 2.79e-31 112
Msa1349130 AT2G24840.1 40.800 125 66 2 1 121 62 182 4.84e-28 105
Msa1349130 AT5G60440.1 40.127 157 75 3 2 154 7 148 9.94e-27 103
Msa1349130 AT1G01530.1 41.727 139 77 2 1 139 6 140 5.03e-26 100
Msa1349130 AT1G65360.1 38.053 113 70 0 1 113 6 118 4.03e-22 89.4
Msa1349130 AT3G57230.2 34.177 158 91 5 1 158 1 145 4.79e-20 82.0
Msa1349130 AT1G47760.1 42.000 100 46 2 1 100 1 88 1.16e-19 82.0
Msa1349130 AT5G13790.2 43.564 101 45 2 1 100 1 90 1.75e-19 83.2
Msa1349130 AT3G57230.1 33.136 169 100 5 1 169 1 156 2.58e-19 82.4
Msa1349130 AT3G57230.4 33.136 169 100 5 1 169 1 156 2.58e-19 82.4
Msa1349130 AT4G36590.1 36.496 137 57 3 2 120 7 131 3.37e-19 82.0
Msa1349130 AT5G13790.1 50.725 69 34 0 1 69 1 69 1.38e-18 80.9
Msa1349130 AT2G22630.1 36.691 139 59 4 1 139 1 110 1.51e-18 80.1
Msa1349130 AT2G22630.2 36.691 139 59 4 1 139 1 110 1.51e-18 80.1
Msa1349130 AT2G45660.2 38.889 108 63 2 1 108 1 105 1.67e-18 78.2
Msa1349130 AT3G61120.1 54.839 62 28 0 1 62 1 62 2.40e-18 79.7
Msa1349130 AT1G72350.1 42.308 130 64 4 2 131 43 161 7.47e-18 78.2
Msa1349130 AT4G09960.2 36.364 143 83 3 1 143 1 135 1.03e-17 77.8
Msa1349130 AT2G45660.1 38.889 108 63 2 1 108 1 105 1.03e-17 77.4
Msa1349130 AT4G09960.1 36.364 143 83 3 1 143 1 135 1.09e-17 77.8
Msa1349130 AT4G37940.1 52.174 69 33 0 1 69 1 69 1.15e-17 77.8
Msa1349130 AT4G09960.6 36.364 143 83 3 1 143 2 136 1.16e-17 77.8
Msa1349130 AT4G22950.2 38.776 98 54 1 1 92 1 98 1.44e-17 76.3
Msa1349130 AT4G37940.4 52.174 69 33 0 1 69 1 69 1.54e-17 77.0
Msa1349130 AT4G37940.3 52.174 69 33 0 1 69 1 69 1.63e-17 76.3
Msa1349130 AT4G09960.5 36.364 143 83 3 1 143 11 145 1.67e-17 77.4
Msa1349130 AT3G58780.1 40.566 106 60 1 1 106 16 118 1.80e-17 77.4
Msa1349130 AT3G57390.3 49.275 69 35 0 1 69 1 69 1.96e-17 77.0
Msa1349130 AT4G09960.3 36.364 143 83 3 1 143 27 161 1.98e-17 77.8
Msa1349130 AT3G58780.5 40.566 106 60 1 1 106 22 124 2.05e-17 77.4
Msa1349130 AT3G58780.4 40.566 106 60 1 1 106 16 118 2.69e-17 77.8
Msa1349130 AT3G04100.1 47.692 65 34 0 2 66 14 78 2.85e-17 76.3
Msa1349130 AT4G09960.4 40.952 105 59 1 1 105 96 197 2.87e-17 78.2
Msa1349130 AT3G57390.1 49.275 69 35 0 1 69 1 69 3.19e-17 77.0
Msa1349130 AT2G42830.1 39.623 106 61 1 1 106 16 118 3.80e-17 76.6
Msa1349130 AT2G42830.2 39.623 106 61 1 1 106 16 118 4.12e-17 76.6
Msa1349130 AT2G22540.3 39.316 117 55 2 1 117 1 101 6.13e-17 75.9
Msa1349130 AT2G22540.1 39.316 117 55 2 1 117 1 101 6.13e-17 75.9
Msa1349130 AT2G45650.1 47.826 69 36 0 1 69 1 69 9.54e-17 75.9
Msa1349130 AT2G14210.1 58.621 58 24 0 1 58 1 58 1.02e-16 75.5
Msa1349130 AT2G14210.2 58.621 58 24 0 1 58 1 58 1.11e-16 75.1
Msa1349130 AT4G22950.3 39.450 109 60 2 1 108 1 104 1.12e-16 75.1
Msa1349130 AT4G22950.1 39.450 109 60 2 1 108 1 104 1.12e-16 75.1
Msa1349130 AT1G29962.1 46.377 69 37 0 2 70 8 76 2.09e-16 73.6
Msa1349130 AT1G28460.1 46.377 69 37 0 2 70 8 76 2.17e-16 73.6
Msa1349130 AT3G58780.2 39.623 106 54 2 1 106 16 111 2.36e-16 74.3
Msa1349130 AT1G22130.1 29.609 179 85 4 1 143 1 174 2.55e-16 75.5
Msa1349130 AT4G24540.1 35.455 110 65 2 1 105 1 109 2.98e-16 73.9
Msa1349130 AT1G77980.1 46.154 78 41 1 1 77 1 78 3.24e-16 75.1
Msa1349130 AT5G65070.4 51.724 58 28 0 1 58 1 58 3.88e-16 72.0
Msa1349130 AT3G54340.1 30.435 184 104 5 1 166 1 178 4.21e-16 73.6
Msa1349130 AT4G11880.2 55.172 58 26 0 1 58 1 58 4.30e-16 72.4
Msa1349130 AT4G11880.3 55.172 58 26 0 1 58 1 58 4.30e-16 72.4
Msa1349130 AT5G10140.2 54.386 57 26 0 1 57 1 57 4.92e-16 72.0
Msa1349130 AT5G51860.2 36.538 104 64 1 1 104 1 102 6.03e-16 72.8
Msa1349130 AT5G51860.1 36.538 104 64 1 1 104 1 102 6.61e-16 72.8
Msa1349130 AT5G20240.2 46.970 66 35 0 1 66 1 66 6.99e-16 71.2
Msa1349130 AT5G10140.4 54.386 57 26 0 1 57 1 57 7.24e-16 72.0
Msa1349130 AT5G65070.1 51.724 58 28 0 1 58 1 58 7.24e-16 72.4
Msa1349130 AT5G10140.3 53.448 58 27 0 1 58 1 58 9.14e-16 72.0
Msa1349130 AT5G10140.1 54.386 57 26 0 1 57 1 57 9.51e-16 72.0
Msa1349130 AT5G62165.17 31.731 104 68 1 1 104 1 101 9.85e-16 71.2
Msa1349130 AT5G62165.12 31.731 104 68 1 1 104 1 101 9.85e-16 71.2
Msa1349130 AT5G62165.11 31.731 104 68 1 1 104 1 101 9.85e-16 71.2
Msa1349130 AT4G11880.4 55.172 58 26 0 1 58 1 58 1.15e-15 72.4
Msa1349130 AT4G11880.1 55.172 58 26 0 1 58 1 58 1.15e-15 72.4
Msa1349130 AT5G20240.1 46.970 66 35 0 1 66 1 66 1.34e-15 72.0
Msa1349130 AT5G62165.6 31.731 104 68 1 1 104 1 101 1.56e-15 70.5
Msa1349130 AT5G62165.9 31.731 104 68 1 1 104 1 101 1.56e-15 70.5
Msa1349130 AT5G62165.7 31.731 104 68 1 1 104 1 101 1.56e-15 70.5
Msa1349130 AT5G62165.8 31.731 104 68 1 1 104 1 101 1.56e-15 70.5
Msa1349130 AT5G62165.13 31.731 104 68 1 1 104 1 101 1.56e-15 70.5
Msa1349130 AT1G46408.1 40.164 122 61 5 1 110 1 122 1.58e-15 72.8
Msa1349130 AT5G65070.3 49.231 65 31 1 1 63 1 65 1.95e-15 72.0
Msa1349130 AT3G30260.1 54.386 57 26 0 1 57 1 57 1.99e-15 72.0
Msa1349130 AT5G65070.2 51.724 58 28 0 1 58 1 58 2.30e-15 71.6
Msa1349130 AT1G77950.3 43.590 78 43 1 1 77 1 78 3.08e-15 71.6
Msa1349130 AT1G77950.2 43.590 78 43 1 1 77 1 78 3.08e-15 71.6
Msa1349130 AT1G77950.1 43.590 78 43 1 1 77 1 78 3.08e-15 71.6
Msa1349130 AT1G77950.4 43.590 78 43 1 1 77 1 78 3.08e-15 71.6
Msa1349130 AT5G65070.5 51.724 58 28 0 1 58 1 58 3.32e-15 70.9
Msa1349130 AT3G58780.3 54.237 59 27 0 1 59 16 74 3.85e-15 71.6
Msa1349130 AT5G51870.2 54.386 57 26 0 1 57 1 57 4.64e-15 69.7
Msa1349130 AT2G22540.2 60.000 50 20 0 1 50 1 50 5.20e-15 70.9
Msa1349130 AT5G62165.10 31.731 104 68 1 1 104 1 101 5.47e-15 70.1
Msa1349130 AT5G62165.4 31.731 104 68 1 1 104 1 101 5.47e-15 70.1
Msa1349130 AT5G62165.3 31.731 104 68 1 1 104 1 101 5.62e-15 70.1
Msa1349130 AT5G62165.1 31.731 104 68 1 1 104 1 101 5.62e-15 70.1
Msa1349130 AT5G62165.2 31.731 104 68 1 1 104 1 101 5.62e-15 70.1
Msa1349130 AT4G18960.1 54.098 61 28 0 2 62 18 78 5.68e-15 70.9
Msa1349130 AT5G51870.1 54.386 57 26 0 1 57 1 57 5.94e-15 70.1
Msa1349130 AT1G69120.2 61.224 49 19 0 1 49 1 49 6.97e-15 70.5
Msa1349130 AT5G51870.5 53.448 58 27 0 1 58 24 81 7.34e-15 69.7
Msa1349130 AT2G03710.3 52.632 57 27 0 1 57 1 57 7.76e-15 69.3
Msa1349130 AT5G51870.4 53.448 58 27 0 1 58 1 58 1.06e-14 69.7
Msa1349130 AT5G60910.1 50.877 57 28 0 1 57 1 57 1.29e-14 69.7
Msa1349130 AT1G69120.1 61.224 49 19 0 1 49 1 49 1.33e-14 70.1
Msa1349130 AT1G71692.1 60.000 50 20 0 1 50 1 50 1.46e-14 68.9
Msa1349130 AT5G51870.3 54.386 57 26 0 1 57 11 67 1.49e-14 69.3
Msa1349130 AT1G26310.1 59.184 49 20 0 1 49 1 49 1.68e-14 69.7
Msa1349130 AT5G15800.1 52.632 57 27 0 1 57 1 57 1.91e-14 69.3
Msa1349130 AT3G02310.2 52.632 57 27 0 1 57 1 57 2.12e-14 68.6
Msa1349130 AT5G23260.3 61.224 49 19 0 1 49 1 49 2.46e-14 68.9
Msa1349130 AT5G23260.2 61.224 49 19 0 1 49 1 49 2.75e-14 68.9
Msa1349130 AT5G15800.2 52.632 57 27 0 1 57 1 57 2.78e-14 69.3
Msa1349130 AT1G18750.4 41.772 79 44 1 1 77 1 79 2.86e-14 70.1
Msa1349130 AT1G18750.1 41.772 79 44 1 1 77 1 79 2.88e-14 70.1
Msa1349130 AT5G23260.4 61.224 49 19 0 1 49 17 65 2.96e-14 69.3
Msa1349130 AT5G23260.1 61.224 49 19 0 1 49 17 65 3.05e-14 68.9
Msa1349130 AT1G18750.3 41.772 79 44 1 1 77 1 79 3.16e-14 70.1
Msa1349130 AT1G24260.3 52.632 57 27 0 1 57 1 57 3.34e-14 68.6
Msa1349130 AT1G24260.1 52.632 57 27 0 1 57 1 57 3.43e-14 68.9
Msa1349130 AT1G24260.2 52.632 57 27 0 1 57 1 57 3.51e-14 68.6
Msa1349130 AT1G69540.1 44.872 78 42 1 1 77 1 78 3.59e-14 69.7
Msa1349130 AT2G03710.1 52.632 57 27 0 1 57 1 57 3.64e-14 68.9
Msa1349130 AT2G03710.2 52.632 57 27 0 1 57 1 57 3.68e-14 68.9
Msa1349130 AT1G69540.2 44.872 78 42 1 1 77 1 78 3.94e-14 69.7
Msa1349130 AT3G02310.1 52.632 57 27 0 1 57 1 57 4.51e-14 68.6
Msa1349130 AT5G65060.3 52.727 55 26 0 1 55 1 55 4.78e-14 67.4
Msa1349130 AT5G62165.14 48.276 58 30 0 1 58 1 58 5.14e-14 65.1
Msa1349130 AT5G65050.2 50.909 55 27 0 1 55 1 55 5.29e-14 67.0
Msa1349130 AT5G65060.2 52.727 55 26 0 1 55 1 55 7.16e-14 66.6
Msa1349130 AT5G65080.3 50.000 56 28 0 1 56 8 63 8.36e-14 66.6
Msa1349130 AT1G17310.1 52.055 73 33 1 2 74 48 118 8.67e-14 67.0
Msa1349130 AT5G65060.1 52.727 55 26 0 1 55 1 55 8.72e-14 66.6
Msa1349130 AT1G17310.2 52.055 73 33 1 2 74 59 129 1.11e-13 67.0
Msa1349130 AT5G65080.2 50.909 55 27 0 1 55 8 62 1.13e-13 66.6
Msa1349130 AT5G65050.5 50.909 55 27 0 1 55 1 55 1.41e-13 65.9
Msa1349130 AT5G65050.3 50.909 55 27 0 1 55 1 55 1.45e-13 66.2
Msa1349130 AT5G65050.1 50.909 55 27 0 1 55 1 55 1.53e-13 65.9
Msa1349130 AT4G37435.1 29.480 173 97 4 1 150 1 171 4.42e-13 64.7
Msa1349130 AT2G03060.3 54.000 50 23 0 1 50 1 50 5.67e-13 66.2
Msa1349130 AT2G03060.7 54.000 50 23 0 1 50 1 50 5.67e-13 66.2
Msa1349130 AT2G03060.1 54.000 50 23 0 1 50 1 50 5.67e-13 66.2
Msa1349130 AT2G03060.2 54.000 50 23 0 1 50 1 50 6.44e-13 66.2
Msa1349130 AT1G28450.1 40.000 70 42 0 2 71 8 77 1.33e-12 63.5
Msa1349130 AT2G26320.1 43.103 58 33 0 1 58 17 74 2.89e-12 60.8
Msa1349130 AT1G60920.1 38.202 89 45 3 3 81 5 93 2.09e-11 60.1
Msa1349130 AT1G77080.11 66.667 39 13 0 1 39 1 39 2.73e-11 59.7
Msa1349130 AT1G77080.5 66.667 39 13 0 1 39 1 39 2.77e-11 59.7
Msa1349130 AT1G77080.12 66.667 39 13 0 1 39 1 39 3.10e-11 58.9
Msa1349130 AT1G77080.4 66.667 39 13 0 1 39 1 39 5.17e-11 59.3
Msa1349130 AT1G77080.2 66.667 39 13 0 1 39 1 39 5.93e-11 58.9

Find 30 sgRNAs with CRISPR-Local

Find 36 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTCTTCTGATTCTTCCAATA+TGG 0.230078 tig0010653:+108175 Msa1349130:CDS
GGTGACAATCCTTACTCTTT+TGG 0.280846 tig0010653:+108077 Msa1349130:CDS
CCTTCAGAGTTCCTACGTTT+AGG 0.407154 tig0010653:-108146 None:intergenic
ATGTATGATGCTTCTAGCTC+AGG 0.444160 tig0010653:+108388 Msa1349130:CDS
TACGTTTAGGCTTTAGTCCA+AGG 0.465520 tig0010653:-108133 None:intergenic
GCAGCAACAACATCAACTCC+TGG 0.473203 tig0010653:-108104 None:intergenic
GCTATTGTTGTGTTCTCTCC+AGG 0.475407 tig0010653:+108056 Msa1349130:CDS
AGGCTTTAGTCCAAGGTGCT+TGG 0.485644 tig0010653:-108126 None:intergenic
GTTGTTGCTGCCAAGCACCT+TGG 0.516004 tig0010653:+108116 Msa1349130:CDS
CCTGGGTGACCAAAAGAGTA+AGG 0.526567 tig0010653:-108086 None:intergenic
ACATTCTCAAAGCGTCGGAC+GGG 0.532546 tig0010653:+107984 Msa1349130:CDS
CACATTCTCAAAGCGTCGGA+CGG 0.538823 tig0010653:+107983 Msa1349130:CDS
TCAAGAACTTGCTGAGCTGT+TGG 0.539928 tig0010653:+108208 Msa1349130:CDS
GAAAGACTCCAACACGAGGC+AGG 0.554822 tig0010653:+107959 Msa1349130:CDS
CCTAAACGTAGGAACTCTGA+AGG 0.584431 tig0010653:+108146 Msa1349130:CDS
GTCGCAAAATTAAGATTGAG+AGG 0.585479 tig0010653:+107934 Msa1349130:CDS
CTAGCTCAGGATCCAGTGGT+TGG 0.586637 tig0010653:+108401 Msa1349130:CDS
GCTTCTAGCTCAGGATCCAG+TGG 0.592947 tig0010653:+108397 Msa1349130:CDS
ATATTGGAAGAATCAGAAGA+GGG 0.595456 tig0010653:-108173 None:intergenic
CAGCAACAACATCAACTCCT+GGG 0.611229 tig0010653:-108103 None:intergenic
GAATGTGACCTGCCTCGTGT+TGG 0.614175 tig0010653:-107967 None:intergenic
CATATTGGAAGAATCAGAAG+AGG 0.623619 tig0010653:-108174 None:intergenic
AGGTCACATTCTCAAAGCGT+CGG 0.637904 tig0010653:+107979 Msa1349130:CDS
GATTCTGAAGCAAGATGATG+TGG 0.645379 tig0010653:+108274 Msa1349130:CDS
CTGTTGGCACAAATCCGAGA+AGG 0.647867 tig0010653:+108224 Msa1349130:CDS
AAAGAGTAAGGATTGTCACC+TGG 0.657655 tig0010653:-108074 None:intergenic
TCGCAAAATTAAGATTGAGA+GGG 0.676979 tig0010653:+107935 Msa1349130:CDS
TATTGGAAGAATCAGAAGAG+GGG 0.697047 tig0010653:-108172 None:intergenic
GGGTGAAAGACTCCAACACG+AGG 0.715970 tig0010653:+107955 Msa1349130:CDS
TTGGACTAAAGCCTAAACGT+AGG 0.730866 tig0010653:+108135 Msa1349130:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTGATTCAATTTTTCCATAT+TGG - tig0010653:108192-108211 None:intergenic 20.0%
TCGCAAAATTAAGATTGAGA+GGG + tig0010653:107935-107954 Msa1349130:CDS 30.0%
!!! AATGAATTGTCGATTTTGTG+CGG + tig0010653:108023-108042 Msa1349130:CDS 30.0%
ATATTGGAAGAATCAGAAGA+GGG - tig0010653:108176-108195 None:intergenic 30.0%
GTCGCAAAATTAAGATTGAG+AGG + tig0010653:107934-107953 Msa1349130:CDS 35.0%
TATTGGAAGAATCAGAAGAG+GGG - tig0010653:108175-108194 None:intergenic 35.0%
CATATTGGAAGAATCAGAAG+AGG - tig0010653:108177-108196 None:intergenic 35.0%
CTCTTCTGATTCTTCCAATA+TGG + tig0010653:108175-108194 Msa1349130:CDS 35.0%
AAAGAGTAAGGATTGTCACC+TGG - tig0010653:108077-108096 None:intergenic 40.0%
! GGTGACAATCCTTACTCTTT+TGG + tig0010653:108077-108096 Msa1349130:CDS 40.0%
! TACGTTTAGGCTTTAGTCCA+AGG - tig0010653:108136-108155 None:intergenic 40.0%
TTGGACTAAAGCCTAAACGT+AGG + tig0010653:108135-108154 Msa1349130:CDS 40.0%
! CAGCTTTCTTTTCACCTTCT+CGG - tig0010653:108241-108260 None:intergenic 40.0%
!! GATTCTGAAGCAAGATGATG+TGG + tig0010653:108274-108293 Msa1349130:CDS 40.0%
!! ATGTATGATGCTTCTAGCTC+AGG + tig0010653:108388-108407 Msa1349130:CDS 40.0%
AGGTCACATTCTCAAAGCGT+CGG + tig0010653:107979-107998 Msa1349130:CDS 45.0%
GCTATTGTTGTGTTCTCTCC+AGG + tig0010653:108056-108075 Msa1349130:CDS 45.0%
CAGCAACAACATCAACTCCT+GGG - tig0010653:108106-108125 None:intergenic 45.0%
CCTTCAGAGTTCCTACGTTT+AGG - tig0010653:108149-108168 None:intergenic 45.0%
CCTAAACGTAGGAACTCTGA+AGG + tig0010653:108146-108165 Msa1349130:CDS 45.0%
!! TCAAGAACTTGCTGAGCTGT+TGG + tig0010653:108208-108227 Msa1349130:CDS 45.0%
!! TTCGCTTTTCTTCCAACCAC+TGG - tig0010653:108416-108435 None:intergenic 45.0%
!! CACATTCTCAAAGCGTCGGA+CGG + tig0010653:107983-108002 Msa1349130:CDS 50.0%
!! ACATTCTCAAAGCGTCGGAC+GGG + tig0010653:107984-108003 Msa1349130:CDS 50.0%
! GATTTTGTGCGGAGCAGAAG+TGG + tig0010653:108034-108053 Msa1349130:CDS 50.0%
CCTGGGTGACCAAAAGAGTA+AGG - tig0010653:108089-108108 None:intergenic 50.0%
! CCTTACTCTTTTGGTCACCC+AGG + tig0010653:108086-108105 Msa1349130:CDS 50.0%
GCAGCAACAACATCAACTCC+TGG - tig0010653:108107-108126 None:intergenic 50.0%
! AGGCTTTAGTCCAAGGTGCT+TGG - tig0010653:108129-108148 None:intergenic 50.0%
CTGTTGGCACAAATCCGAGA+AGG + tig0010653:108224-108243 Msa1349130:CDS 50.0%
GGGTGAAAGACTCCAACACG+AGG + tig0010653:107955-107974 Msa1349130:CDS 55.0%
GAAAGACTCCAACACGAGGC+AGG + tig0010653:107959-107978 Msa1349130:CDS 55.0%
GAATGTGACCTGCCTCGTGT+TGG - tig0010653:107970-107989 None:intergenic 55.0%
GTTGTTGCTGCCAAGCACCT+TGG + tig0010653:108116-108135 Msa1349130:CDS 55.0%
!! GCTTCTAGCTCAGGATCCAG+TGG + tig0010653:108397-108416 Msa1349130:CDS 55.0%
CTAGCTCAGGATCCAGTGGT+TGG + tig0010653:108401-108420 Msa1349130:CDS 55.0%
Chromosome Type Strat End Strand Name
tig0010653 gene 107927 108466 107927 ID=Msa1349130;Name=Msa1349130
tig0010653 mRNA 107927 108466 107927 ID=Msa1349130-mRNA-1;Parent=Msa1349130;Name=Msa1349130-mRNA-1;_AED=0.10;_eAED=0.11;_QI=0|-1|0|1|-1|1|1|0|179
tig0010653 exon 107927 108466 107927 ID=Msa1349130-mRNA-1:exon:6683;Parent=Msa1349130-mRNA-1
tig0010653 CDS 107927 108466 107927 ID=Msa1349130-mRNA-1:cds;Parent=Msa1349130-mRNA-1
Gene Sequence

>Msa1349130

ATGGGTCGTCGCAAAATTAAGATTGAGAGGGTGAAAGACTCCAACACGAGGCAGGTCACATTCTCAAAGCGTCGGACGGGCTTATTCAAGAAAGCAAATGAATTGTCGATTTTGTGCGGAGCAGAAGTGGCTATTGTTGTGTTCTCTCCAGGTGACAATCCTTACTCTTTTGGTCACCCAGGAGTTGATGTTGTTGCTGCCAAGCACCTTGGACTAAAGCCTAAACGTAGGAACTCTGAAGGAAACCCCTCTTCTGATTCTTCCAATATGGAAAAATTGAATCAAGAACTTGCTGAGCTGTTGGCACAAATCCGAGAAGGTGAAAAGAAAGCTGAGACTCATGATGAGATTCTGAAGCAAGATGATGTGGCAAAACTCTCAGAGCTTAAAGAATTGAGAGATTCGTACAAAGATCTTCAAGATTGCATAAAATTGCGTCTCAGTGATATCGAGATATCAGAATGTATGATGCTTCTAGCTCAGGATCCAGTGGTTGGAAGAAAAGCGAAAACGTCAAAAAAGAAAAGAAGAAAAAATTGA
Protein sequence

>Msa1349130

MGRRKIKIERVKDSNTRQVTFSKRRTGLFKKANELSILCGAEVAIVVFSPGDNPYSFGHPGVDVVAAKHLGLKPKRRNSEGNPSSDSSNMEKLNQELAELLAQIREGEKKAETHDEILKQDDVAKLSELKELRDSYKDLQDCIKLRLSDIEISECMMLLAQDPVVGRKAKTSKKKRRKN*