AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1349480

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1349480 MtrunA17_Chr5g0396901 97.312 893 24 0 1 893 25 917 0.0 1804
Msa1349480 MtrunA17_Chr8g0347481 53.333 600 246 8 1 595 1 571 0.0 635
Msa1349480 MtrunA17_Chr4g0042731 29.773 309 168 15 247 539 142 417 8.22e-25 108
Msa1349480 MtrunA17_Chr4g0075541 31.474 251 130 9 280 525 131 344 1.27e-24 107
Msa1349480 MtrunA17_Chr4g0059121 29.845 258 142 10 282 536 104 325 1.18e-23 103
Msa1349480 MtrunA17_Chr4g0065211 30.556 288 150 11 264 535 1139 1392 2.16e-23 107
Msa1349480 MtrunA17_Chr3g0117731 30.233 258 141 9 282 536 103 324 2.22e-23 102
Msa1349480 MtrunA17_Chr5g0406251 26.104 249 146 8 282 528 337 549 5.12e-23 104
Msa1349480 MtrunA17_Chr4g0047191 27.273 253 145 10 282 531 92 308 3.10e-22 99.4
Msa1349480 MtrunA17_Chr4g0047211 27.273 253 145 10 282 531 87 303 3.85e-22 99.0
Msa1349480 MtrunA17_Chr4g0047201 26.616 263 155 9 282 541 248 475 5.44e-22 100
Msa1349480 MtrunA17_Chr2g0305141 29.373 303 166 12 264 549 1101 1372 7.50e-22 102
Msa1349480 MtrunA17_Chr5g0397931 28.013 307 165 16 246 534 421 689 8.25e-22 101
Msa1349480 MtrunA17_Chr1g0211611 26.908 249 144 8 282 528 343 555 8.88e-22 100
Msa1349480 MtrunA17_Chr2g0282321 25.954 262 158 9 282 541 209 436 7.87e-21 96.7
Msa1349480 MtrunA17_Chr6g0461841 31.618 272 152 8 270 536 1001 1243 8.25e-21 99.0
Msa1349480 MtrunA17_Chr6g0450871 28.137 263 143 10 279 526 118 349 1.14e-20 95.1
Msa1349480 MtrunA17_Chr7g0244621 29.134 254 143 9 278 523 82 306 1.60e-20 94.4
Msa1349480 MtrunA17_Chr4g0059181 26.996 263 146 8 279 526 135 366 1.67e-20 94.7
Msa1349480 MtrunA17_Chr3g0143531 27.092 251 145 9 282 530 67 281 1.94e-20 92.8
Msa1349480 MtrunA17_Chr2g0310761 27.687 307 166 12 242 526 933 1205 2.27e-20 97.4
Msa1349480 MtrunA17_Chr3g0117811 25.779 353 192 13 212 526 17 337 2.43e-20 94.0
Msa1349480 MtrunA17_Chr8g0357611 26.687 326 185 11 222 526 1344 1636 3.12e-20 97.1
Msa1349480 MtrunA17_Chr1g0202101 24.315 292 167 10 245 519 273 527 4.46e-20 95.1
Msa1349480 MtrunA17_Chr4g0072741 29.153 295 163 10 247 524 834 1099 6.48e-20 95.9
Msa1349480 MtrunA17_Chr2g0310781 27.213 305 166 13 242 524 839 1109 4.97e-18 89.7
Msa1349480 MtrunA17_Chr4g0068411 28.772 285 152 13 246 516 458 705 5.68e-18 89.0
Msa1349480 MtrunA17_Chr6g0449241 26.316 304 172 12 242 526 20 290 1.25e-17 85.5
Msa1349480 MtrunA17_Chr2g0294351 27.857 280 145 14 265 528 485 723 8.52e-17 85.5
Msa1349480 MtrunA17_Chr3g0090261 25.101 247 146 9 280 523 126 336 1.35e-16 82.8
Msa1349480 MtrunA17_Chr3g0116981 25.413 303 169 11 242 523 20 286 1.42e-16 82.4
Msa1349480 MtrunA17_Chr1g0196631 28.629 248 139 7 289 530 955 1170 1.81e-16 84.7
Msa1349480 MtrunA17_Chr5g0403721 26.531 245 150 9 279 523 28 242 4.05e-16 81.3
Msa1349480 MtrunA17_Chr1g0171571 28.696 230 115 9 326 526 106 315 5.34e-16 82.4
Msa1349480 MtrunA17_Chr5g0443471 27.881 269 156 11 280 541 205 442 9.29e-16 81.3
Msa1349480 MtrunA17_Chr5g0394681 27.461 193 111 6 334 526 201 364 4.52e-15 78.2
Msa1349480 MtrunA17_Chr1g0191171 24.252 301 173 13 246 530 687 948 5.45e-15 79.7
Msa1349480 MtrunA17_Chr5g0427651 25.581 301 165 15 247 528 645 905 1.09e-14 78.6
Msa1349480 MtrunA17_Chr1g0157231 25.789 190 105 6 282 470 188 342 1.93e-14 76.3
Msa1349480 MtrunA17_Chr8g0380551 28.226 248 136 11 282 519 266 481 3.55e-14 76.3
Msa1349480 MtrunA17_Chr1g0179331 24.497 298 172 12 247 528 554 814 8.09e-14 75.9
Msa1349480 MtrunA17_Chr3g0105891 25.820 244 142 8 285 523 371 580 1.29e-13 74.7
Msa1349480 MtrunA17_Chr5g0437741 29.694 229 114 9 282 509 536 718 1.80e-13 74.7
Msa1349480 MtrunA17_Chr4g0000431 23.288 292 182 10 243 521 19 281 2.29e-13 74.3
Msa1349480 MtrunA17_Chr1g0209511 26.766 269 150 11 277 524 352 594 5.88e-13 72.8
Msa1349480 MtrunA17_Chr3g0115171 26.778 239 137 8 277 513 322 524 1.51e-12 71.2
Msa1349480 MtrunA17_Chr1g0171451 28.000 275 149 13 265 526 433 671 1.80e-12 71.2
Msa1349480 MtrunA17_Chr4g0051191 27.273 242 134 8 277 514 203 406 3.86e-12 69.3
Msa1349480 MtrunA17_Chr6g0450571 23.675 283 178 9 243 514 11 266 6.30e-12 69.3
Msa1349480 MtrunA17_Chr8g0359691 26.733 303 147 16 255 523 674 935 7.03e-12 69.7
Msa1349480 MtrunA17_Chr4g0053411 24.702 336 170 14 239 523 458 761 8.04e-12 69.3
Msa1349480 MtrunA17_Chr2g0280191 26.190 252 131 11 241 469 676 895 1.24e-11 68.9
Msa1349480 MtrunA17_Chr1g0209521 24.752 303 166 13 255 527 368 638 1.83e-11 68.2
Msa1349480 MtrunA17_Chr8g0384301 26.293 232 133 8 285 514 349 544 3.27e-11 67.4
Msa1349480 MtrunA17_Chr4g0041201 26.316 285 160 13 257 531 559 803 4.53e-11 67.0
Msa1349480 MtrunA17_Chr5g0423121 25.769 260 144 11 277 521 377 602 5.61e-11 66.2
Msa1349480 MtrunA17_Chr7g0276571 26.592 267 138 12 280 523 457 688 6.50e-11 66.2
Msa1349480 MtrunA17_Chr3g0111551 26.728 217 122 8 285 499 113 294 7.69e-11 64.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1349480 AT1G64300.2 52.510 777 297 14 1 767 1 715 0.0 785
Msa1349480 AT1G64300.1 52.510 777 297 14 1 767 1 715 0.0 785
Msa1349480 AT5G41730.2 52.291 742 295 11 1 726 1 699 0.0 764
Msa1349480 AT5G41730.1 52.291 742 295 11 1 726 1 699 0.0 764
Msa1349480 AT1G01450.1 53.590 390 151 7 211 594 2 367 1.45e-131 403
Msa1349480 AT1G14000.1 31.392 309 165 17 247 540 161 437 1.82e-24 107
Msa1349480 AT1G62400.1 30.645 248 131 8 280 523 130 340 5.47e-24 105
Msa1349480 AT3G46930.2 25.959 339 198 10 224 545 179 481 7.60e-24 107
Msa1349480 AT3G46930.1 25.959 339 198 10 224 545 144 446 1.02e-23 106
Msa1349480 AT3G46920.2 28.378 370 191 16 192 530 816 1142 1.09e-22 105
Msa1349480 AT3G46920.1 28.378 370 191 16 192 530 750 1076 1.18e-22 105
Msa1349480 AT5G58950.1 27.451 255 140 11 276 523 247 463 1.56e-21 100
Msa1349480 AT2G35050.1 28.618 304 166 14 242 526 968 1239 4.23e-21 100
Msa1349480 AT2G35050.2 28.618 304 166 14 242 526 968 1239 4.23e-21 100
Msa1349480 AT5G01850.1 27.642 246 145 7 282 523 63 279 1.15e-20 94.7
Msa1349480 AT4G38470.2 25.794 252 149 8 282 531 332 547 2.81e-20 96.3
Msa1349480 AT5G57610.1 27.723 303 173 10 247 532 780 1053 3.88e-20 97.1
Msa1349480 AT5G01850.2 29.907 214 120 6 314 523 116 303 1.10e-19 92.4
Msa1349480 AT4G35780.1 24.590 244 146 8 282 523 334 541 1.33e-19 94.4
Msa1349480 AT4G38470.1 25.506 247 146 8 282 526 332 542 1.63e-19 94.0
Msa1349480 AT5G50000.1 26.616 263 147 8 279 526 128 359 1.84e-19 92.0
Msa1349480 AT1G04700.1 30.380 237 137 6 289 524 821 1030 2.27e-19 94.4
Msa1349480 AT1G04700.2 30.380 237 137 6 289 524 821 1030 2.27e-19 94.4
Msa1349480 AT3G01490.1 28.244 262 144 11 279 526 154 385 2.28e-19 92.0
Msa1349480 AT2G17700.1 26.000 250 145 10 282 528 328 540 2.86e-19 93.2
Msa1349480 AT1G79570.2 26.403 303 175 10 242 526 958 1230 3.57e-19 94.0
Msa1349480 AT1G79570.1 26.403 303 175 10 242 526 958 1230 3.57e-19 94.0
Msa1349480 AT1G16270.1 27.143 280 167 8 258 533 890 1136 4.03e-19 93.6
Msa1349480 AT1G16270.2 27.143 280 167 8 258 533 890 1136 4.03e-19 93.6
Msa1349480 AT1G16270.3 27.143 280 167 8 258 533 890 1136 4.03e-19 93.6
Msa1349480 AT3G24715.1 28.873 284 172 9 243 522 842 1099 4.40e-19 93.6
Msa1349480 AT3G24715.3 28.873 284 172 9 243 522 842 1099 4.40e-19 93.6
Msa1349480 AT3G24715.2 28.873 284 172 9 243 522 842 1099 4.40e-19 93.6
Msa1349480 AT4G31170.7 27.092 251 136 9 280 523 175 385 5.92e-19 90.9
Msa1349480 AT4G31170.6 27.092 251 136 9 280 523 175 385 5.92e-19 90.9
Msa1349480 AT4G31170.5 27.092 251 136 9 280 523 175 385 5.92e-19 90.9
Msa1349480 AT4G31170.1 27.092 251 136 9 280 523 175 385 5.92e-19 90.9
Msa1349480 AT4G31170.2 27.092 251 136 9 280 523 175 385 5.92e-19 90.9
Msa1349480 AT4G31170.3 27.092 251 136 9 280 523 175 385 5.92e-19 90.9
Msa1349480 AT4G31170.8 27.092 251 136 9 280 523 175 385 5.92e-19 90.9
Msa1349480 AT3G50730.1 25.403 248 156 8 277 523 77 296 2.20e-18 88.6
Msa1349480 AT3G06630.2 28.283 297 160 15 247 528 433 691 2.71e-18 90.5
Msa1349480 AT4G23050.2 27.815 302 158 14 246 528 466 726 2.73e-18 90.5
Msa1349480 AT4G23050.1 27.815 302 158 14 246 528 465 725 2.77e-18 90.5
Msa1349480 AT5G66710.1 25.641 273 163 8 278 544 113 351 4.37e-18 88.2
Msa1349480 AT2G40860.4 30.964 197 98 6 340 514 24 204 9.53e-18 88.2
Msa1349480 AT5G49470.3 27.857 280 145 14 265 528 510 748 1.06e-17 88.6
Msa1349480 AT5G49470.4 27.857 280 145 14 265 528 484 722 1.22e-17 88.6
Msa1349480 AT2G40860.2 30.144 209 108 6 340 526 122 314 1.39e-17 88.2
Msa1349480 AT3G27560.1 27.600 250 146 10 282 526 71 290 1.47e-17 85.9
Msa1349480 AT5G11850.1 25.503 298 171 12 246 529 607 867 1.53e-17 88.2
Msa1349480 AT2G40860.1 30.964 197 98 6 340 514 122 302 1.55e-17 87.8
Msa1349480 AT5G49470.7 27.857 280 145 14 265 528 383 621 1.71e-17 87.8
Msa1349480 AT2G40860.3 30.964 197 98 6 340 514 122 302 1.72e-17 87.8
Msa1349480 AT5G49470.1 27.857 280 145 14 265 528 223 461 2.25e-17 86.7
Msa1349480 AT5G40540.1 27.668 253 148 11 276 523 64 286 2.35e-17 85.1
Msa1349480 AT2G24360.1 26.050 238 143 7 286 523 180 384 2.43e-17 85.9
Msa1349480 AT3G22750.1 24.528 265 151 8 279 526 120 352 3.75e-17 85.1
Msa1349480 AT3G57830.3 27.737 274 162 10 279 527 398 660 5.85e-17 86.3
Msa1349480 AT3G57830.1 27.737 274 162 10 279 527 398 660 5.85e-17 86.3
Msa1349480 AT4G18950.2 28.571 252 138 10 282 526 200 416 9.52e-17 84.3
Msa1349480 AT4G18950.1 28.571 252 138 10 282 526 200 416 1.07e-16 84.3
Msa1349480 AT3G63260.1 24.060 266 155 8 279 526 129 365 1.10e-16 83.6
Msa1349480 AT4G24480.1 26.073 303 166 13 247 528 668 933 1.54e-16 85.1
Msa1349480 AT2G42290.1 28.731 268 159 9 282 527 389 646 1.92e-16 84.3
Msa1349480 AT5G50180.1 27.236 246 145 9 282 523 65 280 2.04e-16 82.4
Msa1349480 AT1G67890.1 27.273 275 152 13 265 528 506 743 2.09e-16 84.7
Msa1349480 AT5G40540.3 29.439 214 120 9 314 523 3 189 3.68e-16 79.7
Msa1349480 AT4G24480.2 25.589 297 170 13 247 528 668 928 4.71e-16 83.6
Msa1349480 AT4G24480.3 25.589 297 170 13 247 528 668 928 5.91e-16 83.2
Msa1349480 AT5G40540.2 29.439 214 120 9 314 523 24 210 7.35e-16 79.3
Msa1349480 AT3G06620.1 27.798 277 149 13 265 528 513 751 7.84e-16 82.8
Msa1349480 AT3G50720.1 26.848 257 159 9 276 531 88 316 1.63e-15 80.1
Msa1349480 AT3G58760.1 29.000 300 156 18 247 526 165 427 6.68e-15 79.0
Msa1349480 AT3G58760.3 29.000 300 156 18 247 526 228 490 8.60e-15 79.0
Msa1349480 AT3G58760.5 29.000 300 156 18 247 526 228 490 8.60e-15 79.0
Msa1349480 AT3G06630.1 28.261 276 145 15 247 507 433 670 8.90e-15 79.0
Msa1349480 AT4G14780.1 26.316 190 109 5 339 526 178 338 1.04e-14 77.4
Msa1349480 AT3G46930.4 24.715 263 149 8 224 473 179 405 3.26e-14 76.3
Msa1349480 AT3G46930.3 24.715 263 149 8 224 473 179 405 3.44e-14 76.3
Msa1349480 AT2G31800.1 25.987 304 177 14 243 530 188 459 3.99e-14 76.3
Msa1349480 AT3G46930.5 24.715 263 149 8 224 473 144 370 5.60e-14 75.5
Msa1349480 AT3G46930.6 24.715 263 149 8 224 473 83 309 7.38e-14 74.3
Msa1349480 AT3G58640.1 28.205 273 146 13 257 519 565 797 8.96e-14 76.3
Msa1349480 AT3G58640.2 28.205 273 146 13 257 519 565 797 8.96e-14 76.3
Msa1349480 AT1G50240.3 24.211 285 161 12 243 513 1 244 2.08e-13 75.1
Msa1349480 AT1G50240.2 24.211 285 161 12 243 513 1 244 2.08e-13 75.1
Msa1349480 AT5G66850.1 25.703 249 153 10 283 530 394 611 3.25e-13 74.3
Msa1349480 AT5G01890.1 23.743 358 209 13 191 527 646 960 3.44e-13 74.3
Msa1349480 AT5G66850.2 25.703 249 153 10 283 530 394 611 4.23e-13 73.9
Msa1349480 AT3G06630.4 28.571 238 120 13 247 470 433 634 4.89e-13 73.6
Msa1349480 AT3G06630.3 28.571 238 120 13 247 470 433 634 4.89e-13 73.6
Msa1349480 AT3G06630.5 28.571 238 120 13 247 470 433 634 4.89e-13 73.6
Msa1349480 AT3G59830.1 27.586 290 162 13 247 519 193 451 5.14e-13 72.8
Msa1349480 AT3G06630.6 28.571 238 120 13 247 470 349 550 5.60e-13 73.2
Msa1349480 AT5G18700.1 25.847 236 142 10 285 520 46 248 7.89e-13 73.2
Msa1349480 AT1G67890.3 27.907 258 130 15 265 507 506 722 3.25e-12 70.9
Msa1349480 AT1G67890.2 27.907 258 130 15 265 507 506 722 3.25e-12 70.9
Msa1349480 AT1G67890.4 27.907 258 130 15 265 507 378 594 4.24e-12 70.5
Msa1349480 AT2G31010.1 25.685 292 159 13 257 538 531 774 5.09e-12 70.5
Msa1349480 AT2G31010.2 25.685 292 159 13 257 538 531 774 5.09e-12 70.5
Msa1349480 AT3G53930.2 27.586 261 134 13 247 491 19 240 6.29e-12 70.1
Msa1349480 AT3G53930.1 27.586 261 134 13 247 491 19 240 6.67e-12 70.1
Msa1349480 AT5G49470.5 28.182 220 104 12 265 469 510 690 9.86e-12 69.3
Msa1349480 AT5G49470.6 28.182 220 104 12 265 469 510 690 1.08e-11 69.3
Msa1349480 AT2G42640.1 28.044 271 141 13 265 524 547 774 1.15e-11 69.3
Msa1349480 AT2G42640.4 28.044 271 141 13 265 524 509 736 1.39e-11 68.9
Msa1349480 AT5G38210.3 24.739 287 162 10 280 544 391 645 1.69e-11 68.6
Msa1349480 AT5G38210.2 25.175 286 162 10 280 544 409 663 1.75e-11 68.6
Msa1349480 AT5G07140.1 26.014 296 177 13 242 528 321 583 2.79e-11 67.8
Msa1349480 AT3G01490.2 27.632 228 122 10 279 492 154 352 3.19e-11 66.6
Msa1349480 AT4G08480.1 26.172 256 147 9 261 514 529 744 3.25e-11 67.8
Msa1349480 AT1G69270.1 25.820 244 144 10 270 510 291 500 4.83e-11 67.0
Msa1349480 AT5G40540.4 28.061 196 114 8 276 469 64 234 5.18e-11 65.1
Msa1349480 AT5G38210.1 25.175 286 162 10 280 544 397 651 5.51e-11 67.0
Msa1349480 AT4G08500.1 26.336 262 153 9 255 514 353 576 6.00e-11 66.6
Msa1349480 AT3G02880.1 31.500 200 99 9 272 467 375 540 6.09e-11 66.6
Msa1349480 AT3G19700.1 28.497 193 105 8 282 469 730 894 7.65e-11 66.6

Find 163 sgRNAs with CRISPR-Local

Find 205 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCTCTCCTGCTTTGATATTT+TGG 0.080066 tig0010964:+14799 Msa1349480:intergenic
CTCTCAGATTCTGAGACTTT+TGG 0.146039 tig0010964:+13965 Msa1349480:intergenic
ATCAAACTCACAAGATATTT+TGG 0.164131 tig0010964:+14753 Msa1349480:intergenic
TTGATTCCCTTTGTTAATAT+TGG 0.196770 tig0010964:+13937 Msa1349480:intergenic
CAAGCAAAAGTTGTTGGATT+TGG 0.204645 tig0010964:-15080 Msa1349480:CDS
GAGATTTGGCAAACAATATT+TGG 0.205668 tig0010964:-15753 Msa1349480:CDS
GATCCTAACAAAACACTAAT+TGG 0.207436 tig0010964:+15524 Msa1349480:intergenic
AATTGCTTGCAAATTTCTTT+TGG 0.212048 tig0010964:+15728 Msa1349480:intergenic
TCTCCTCACTTGGTAATCTT+TGG 0.230174 tig0010964:+15502 Msa1349480:intergenic
GCTTATAAAGTTGTTGAAAA+AGG 0.240086 tig0010964:-14480 Msa1349480:CDS
GTGTCATCAGAAATTGATTT+TGG 0.241755 tig0010964:+14285 Msa1349480:intergenic
GTAGGATATTACAAGGATTA+AGG 0.243595 tig0010964:+15135 Msa1349480:intergenic
TATTCCTACTATAGATAATA+AGG 0.252638 tig0010964:-14424 Msa1349480:CDS
AAGAGATTAACCCTATAATT+TGG 0.257480 tig0010964:-14986 Msa1349480:CDS
GTCCTCTTAGAGAGCTTTCC+AGG 0.263161 tig0010964:-16036 Msa1349480:CDS
AACTGACCTTGGTCTTTCTT+TGG 0.265277 tig0010964:+14195 Msa1349480:intergenic
GGTTACCAGATCTTTAATTA+TGG 0.266121 tig0010964:+14311 Msa1349480:intergenic
CATAGATATTGCAATATGTT+TGG 0.282076 tig0010964:+15353 Msa1349480:intergenic
TCAGGTGCATACCAAATTAT+AGG 0.292831 tig0010964:+14975 Msa1349480:intergenic
CTGGTTTCTCATCTATTTGT+AGG 0.294229 tig0010964:-14695 Msa1349480:CDS
TTTGTCTTTAACCTGTTAAA+TGG 0.297516 tig0010964:+13812 Msa1349480:intergenic
CGGCACATTGGTGAGGTTCT+TGG 0.303933 tig0010964:-16223 Msa1349480:CDS
TATGGTCATGTCTCAGATTA+AGG 0.304997 tig0010964:-13485 None:intergenic
TGGAAGTCTAAAAGCGCTTA+TGG 0.306735 tig0010964:-16203 Msa1349480:CDS
TGAAACATGGCAGCATCTTC+TGG 0.314429 tig0010964:+13655 Msa1349480:intergenic
AAAAGAAATTTGCAAGCAAT+TGG 0.319977 tig0010964:-15726 Msa1349480:CDS
AATATACATTAAGCATTGTT+TGG 0.325498 tig0010964:-15996 Msa1349480:CDS
GGATTGTCATCTGTTGAAAA+TGG 0.333696 tig0010964:-15059 Msa1349480:CDS
ATTCTTTGAAGAAGTGAGTT+TGG 0.334226 tig0010964:+15640 Msa1349480:intergenic
GTCCTTTGATTGCTCACATA+TGG 0.339488 tig0010964:+13569 Msa1349480:intergenic
AGCATTGTGAGGATGCAAAA+AGG 0.341134 tig0010964:-13719 Msa1349480:CDS
CATTATCTGGTCGGGTTTCA+AGG 0.346406 tig0010964:-14051 Msa1349480:CDS
TTGACACGTCTTATTCTTGT+TGG 0.355333 tig0010964:+13755 Msa1349480:intergenic
GAAATTGATGAATGGTAATT+CGG 0.363355 tig0010964:-15588 Msa1349480:CDS
TCGGGTTTCAAGGACAAATA+AGG 0.364030 tig0010964:-14041 Msa1349480:CDS
TTTAAGGAAATTCAATGGTT+AGG 0.370820 tig0010964:-15458 Msa1349480:CDS
GAGACCAATCACCATTTAAC+AGG 0.371640 tig0010964:-13823 Msa1349480:CDS
ATTGTTTGGATTCAAAAGAT+TGG 0.378122 tig0010964:-15982 Msa1349480:CDS
GTGAGAAAGTGATAACAAAT+TGG 0.378799 tig0010964:+15376 Msa1349480:intergenic
TTCTTTGGCTTCATTTGTGA+TGG 0.379395 tig0010964:+14210 Msa1349480:intergenic
TTAACCTGTTAAATGGTGAT+TGG 0.382051 tig0010964:+13819 Msa1349480:intergenic
ATAGATATTGCAATATGTTT+GGG 0.387928 tig0010964:+15354 Msa1349480:intergenic
AGTTCAAGCTGATTTAGTTC+AGG 0.389760 tig0010964:+14615 Msa1349480:intergenic
ATCTCATGCTTCAAATGGCC+AGG 0.399868 tig0010964:-15199 Msa1349480:CDS
TTGAAGACAGAACTACAAAA+TGG 0.399911 tig0010964:-16072 Msa1349480:CDS
ATTGTAACATTCTTCTTTGA+CGG 0.401100 tig0010964:+14240 Msa1349480:intergenic
TTGGATTCAAAAGATTGGTC+TGG 0.401687 tig0010964:-15977 Msa1349480:CDS
TGAGGAGAAGATTGGGAAGG+GGG 0.403016 tig0010964:-15487 Msa1349480:CDS
GGGTTAATCTCTTCACCAAT+TGG 0.403263 tig0010964:+14996 Msa1349480:intergenic
CCATATGTTGTTCTTACATA+AGG 0.409101 tig0010964:+13503 Msa1349480:intergenic
TACCAAGTGAGGAGAAGATT+GGG 0.409714 tig0010964:-15494 Msa1349480:CDS
ACAGTAGCCACATCCTTTGT+TGG 0.413590 tig0010964:+14387 Msa1349480:intergenic
TCATTACCACCAACATTATC+TGG 0.424357 tig0010964:-14064 Msa1349480:CDS
ATGCTGCCATGTTTCAGATA+TGG 0.424537 tig0010964:-13648 Msa1349480:CDS
GAGAATGCTGGGGAGATGTC+AGG 0.427765 tig0010964:-15863 Msa1349480:CDS
TGGATTCAAAAGATTGGTCT+GGG 0.432597 tig0010964:-15976 Msa1349480:CDS
TTATAAGCAAACATTTCATA+AGG 0.434328 tig0010964:+14495 Msa1349480:intergenic
CATCTTCACAAACTGTGCTT+TGG 0.436780 tig0010964:+14343 Msa1349480:intergenic
TGCATCCATAATTAAAGATC+TGG 0.439192 tig0010964:-14316 Msa1349480:CDS
TTGTAACATTCTTCTTTGAC+GGG 0.441892 tig0010964:+14241 Msa1349480:intergenic
CTCAAAGGGCTCAATGCTAA+TGG 0.444928 tig0010964:+13527 Msa1349480:intergenic
CTCAAAGGACTCAATGGAAA+TGG 0.446507 tig0010964:+13593 Msa1349480:intergenic
TTCCCAATCTTCTCCTCACT+TGG 0.447928 tig0010964:+15492 Msa1349480:intergenic
ATTCCTACTATAGATAATAA+GGG 0.450157 tig0010964:-14423 Msa1349480:CDS
TGAAAATGATTATCCTCAAA+AGG 0.452553 tig0010964:+14837 Msa1349480:intergenic
GCAAGCAATTGGAGAATGCT+TGG 0.459665 tig0010964:-15715 Msa1349480:CDS
GAGAACAAAATCTGTCTCCT+TGG 0.464084 tig0010964:+15239 Msa1349480:intergenic
GGAGATGTCAGGGTTGGATC+AGG 0.464317 tig0010964:-15852 Msa1349480:CDS
TTACCAAGTGAGGAGAAGAT+TGG 0.469923 tig0010964:-15495 Msa1349480:CDS
AGGAATTTAAGGAAATTCAA+TGG 0.476317 tig0010964:-15463 Msa1349480:CDS
ACCACCAACATTATCTGGTC+GGG 0.478543 tig0010964:-14059 Msa1349480:CDS
CACCGCCAATGAGTCCTTCA+AGG 0.481111 tig0010964:-13685 Msa1349480:CDS
AAAGCCGATGCATATAGCTT+TGG 0.483467 tig0010964:-14894 Msa1349480:CDS
CAGGTGCATACCAAATTATA+GGG 0.486379 tig0010964:+14976 Msa1349480:intergenic
TCACCAACACATAATCCAAT+TGG 0.489553 tig0010964:-15011 Msa1349480:CDS
TACAAGGATTAAGGTTTCCA+TGG 0.498240 tig0010964:+15144 Msa1349480:intergenic
TATGGACTCAAAGGACTCAA+TGG 0.498751 tig0010964:+13587 Msa1349480:intergenic
TTCACCTTCTTTGAATACCC+TGG 0.500775 tig0010964:+16018 Msa1349480:intergenic
CTACCCTTATTATCTATAGT+AGG 0.503856 tig0010964:+14420 Msa1349480:intergenic
ACCAACCAAAATATCAAAGC+AGG 0.507591 tig0010964:-14804 Msa1349480:CDS
GTTCTTACATAAGGACTCAA+AGG 0.508350 tig0010964:+13512 Msa1349480:intergenic
ATTGAAGCGATCGAGAATGC+TGG 0.508806 tig0010964:-15875 Msa1349480:CDS
CTTTCCAGGGTATTCAAAGA+AGG 0.511780 tig0010964:-16022 Msa1349480:CDS
TCTAGCTCAGTTAAAACCTC+AGG 0.514317 tig0010964:+14957 Msa1349480:intergenic
TTGAAGCGATCGAGAATGCT+GGG 0.515187 tig0010964:-15874 Msa1349480:CDS
ACTGATCCGTCTCAGCGTCC+TGG 0.518950 tig0010964:-14714 Msa1349480:CDS
CTTCTGGTGTATGACCTTGA+AGG 0.519264 tig0010964:+13671 Msa1349480:intergenic
AGAATGCTTGGAGAGAAGAT+AGG 0.519476 tig0010964:-15703 Msa1349480:CDS
AGTTGATGAACAAGGATTTG+TGG 0.524686 tig0010964:-15286 Msa1349480:CDS
TGCTGGGGAGATGTCAGGGT+TGG 0.526191 tig0010964:-15858 Msa1349480:CDS
GGAAATCCAATATTAACAAA+GGG 0.528711 tig0010964:-13943 Msa1349480:CDS
CCTTATGTAAGAACAACATA+TGG 0.529072 tig0010964:-13503 Msa1349480:CDS
CTATAACCCCAGTTAAAAGA+AGG 0.532741 tig0010964:-13991 Msa1349480:CDS
AGGAAATCCAATATTAACAA+AGG 0.532942 tig0010964:-13944 Msa1349480:CDS
ATATTATATGTCCTCTCCAC+AGG 0.535682 tig0010964:+14537 Msa1349480:intergenic
TATGACCTTGAAGGACTCAT+TGG 0.538589 tig0010964:+13680 Msa1349480:intergenic
CAAGAAAATATGATGTGGAA+TGG 0.539842 tig0010964:-15799 Msa1349480:CDS
ACACAATCGCAGATGAGATA+AGG 0.544064 tig0010964:-16108 Msa1349480:CDS
GTGCCGGAATGGAACAGCTC+CGG 0.546196 tig0010964:-16243 None:intergenic
TTTGTTCTCTATACCTGTGG+TGG 0.547282 tig0010964:-15225 Msa1349480:CDS
GTAGATTGTTGTGACATTGA+AGG 0.548073 tig0010964:-14579 Msa1349480:CDS
ATGCTTGGAGAGAAGATAGG+TGG 0.548742 tig0010964:-15700 Msa1349480:CDS
TATAATTTGGTATGCACCTG+AGG 0.550991 tig0010964:-14973 Msa1349480:CDS
TTCTTACATAAGGACTCAAA+GGG 0.551714 tig0010964:+13513 Msa1349480:intergenic
GGTTGATCTCATGCTTCAAA+TGG 0.555778 tig0010964:-15204 Msa1349480:CDS
AACATATTGCAATATCTATG+TGG 0.560041 tig0010964:-15350 Msa1349480:CDS
ACCCTGGAAAGCTCTCTAAG+AGG 0.560256 tig0010964:+16034 Msa1349480:intergenic
ATGGAACAGCTCCGGCACAT+TGG 0.561500 tig0010964:-16235 Msa1349480:CDS
AGCAATCAAAGGACTAGAAG+AGG 0.562315 tig0010964:-13560 Msa1349480:CDS
GGCGAGCGATATGGAAACAA+AGG 0.564210 tig0010964:+14774 Msa1349480:intergenic
CAAGAACCTCACCAATGTGC+CGG 0.564531 tig0010964:+16224 Msa1349480:intergenic
GTGGAGATATTCAATGCCCC+TGG 0.567677 tig0010964:+15181 Msa1349480:intergenic
TCAATGTCACAACAATCTAC+AGG 0.568956 tig0010964:+14582 Msa1349480:intergenic
GACCTTGAAGGACTCATTGG+CGG 0.570717 tig0010964:+13683 Msa1349480:intergenic
TTGTTGAAGAAATTGATGAA+TGG 0.570718 tig0010964:-15596 Msa1349480:CDS
TCACCAATTGGATTATGTGT+TGG 0.571772 tig0010964:+15008 Msa1349480:intergenic
GAGAAACCAGGACGCTGAGA+CGG 0.572704 tig0010964:+14708 Msa1349480:intergenic
TACCACCAACATTATCTGGT+CGG 0.572730 tig0010964:-14060 Msa1349480:CDS
GTCTGAGCCAACAAAGGATG+TGG 0.574953 tig0010964:-14394 Msa1349480:CDS
TTGCTCACATATGGACTCAA+AGG 0.582014 tig0010964:+13578 Msa1349480:intergenic
TCTCATGCTTCAAATGGCCA+GGG 0.583448 tig0010964:-15198 Msa1349480:CDS
AGTGAGGAGAAGATTGGGAA+GGG 0.587219 tig0010964:-15489 Msa1349480:CDS
TCGATCGCTTCAATAACCGC+AGG 0.587690 tig0010964:+15884 Msa1349480:intergenic
GTGAGGAGAAGATTGGGAAG+GGG 0.591731 tig0010964:-15488 Msa1349480:CDS
GAGGCTCATAGAAGCACTCA+AGG 0.595114 tig0010964:-15678 Msa1349480:CDS
GTCCATATGTGAGCAATCAA+AGG 0.596259 tig0010964:-13571 Msa1349480:CDS
CATACCAAAGCTATATGCAT+CGG 0.599646 tig0010964:+14890 Msa1349480:intergenic
AAAGCTAGAAACTCACAAGA+AGG 0.600879 tig0010964:-15110 Msa1349480:CDS
AACTTGATGTCAATGATGAC+TGG 0.601724 tig0010964:+14014 Msa1349480:intergenic
AGAATGCTGGGGAGATGTCA+GGG 0.601811 tig0010964:-15862 Msa1349480:CDS
TGAGAGAGAATTGTGGTCCA+AGG 0.604253 tig0010964:-15256 Msa1349480:CDS
TCCTCTTAGAGAGCTTTCCA+GGG 0.606681 tig0010964:-16035 Msa1349480:CDS
TTTCTCTGAGTACTTACAAG+AGG 0.613541 tig0010964:+14914 Msa1349480:intergenic
ACCCGACCAGATAATGTTGG+TGG 0.618092 tig0010964:+14058 Msa1349480:intergenic
AGCATGAGATCAACCACCAC+AGG 0.618436 tig0010964:+15212 Msa1349480:intergenic
CTTGGAGAGAAGATAGGTGG+AGG 0.618932 tig0010964:-15697 Msa1349480:CDS
AAAATATCAAAGCAGGAGAG+AGG 0.620693 tig0010964:-14797 Msa1349480:CDS
GAAACCCGACCAGATAATGT+TGG 0.622199 tig0010964:+14055 Msa1349480:intergenic
CTACAAATAGATGAGAAACC+AGG 0.623694 tig0010964:+14696 Msa1349480:intergenic
AACTATCCATATCTGAAACA+TGG 0.625924 tig0010964:+13642 Msa1349480:intergenic
ACAGTAATGATAAGGTATTG+TGG 0.632731 tig0010964:-15547 Msa1349480:CDS
ATGTCACAACAATCTACAGG+TGG 0.639302 tig0010964:+14585 Msa1349480:intergenic
ATGCTAGAGTGAATATGTCG+TGG 0.640538 tig0010964:+16134 Msa1349480:intergenic
AGGATTGATCAAAACATACT+CGG 0.640765 tig0010964:+14635 Msa1349480:intergenic
CAAAAGTCTCAGAATCTGAG+AGG 0.650694 tig0010964:-13964 Msa1349480:CDS
TCTCTAAGAGGACATTCAAG+AGG 0.651164 tig0010964:+16046 Msa1349480:intergenic
ACAGCTCCGGCACATTGGTG+AGG 0.651948 tig0010964:-16230 Msa1349480:CDS
GCTTGCAAGAAAATATGATG+TGG 0.653459 tig0010964:-15804 Msa1349480:CDS
TAGTGAGTCTGAGCCAACAA+AGG 0.653550 tig0010964:-14400 Msa1349480:CDS
CGATCGCTTCAATAACCGCA+GGG 0.654532 tig0010964:+15885 Msa1349480:intergenic
AAGTGAGGAGAAGATTGGGA+AGG 0.655591 tig0010964:-15490 Msa1349480:CDS
AATTGGATTATGTGTTGGTG+AGG 0.655784 tig0010964:+15013 Msa1349480:intergenic
TGTGACGGAGTTGATGAACA+AGG 0.656381 tig0010964:-15294 Msa1349480:CDS
CTCATGCTTCAAATGGCCAG+GGG 0.668271 tig0010964:-15197 Msa1349480:CDS
GATCCAAAGATTACCAAGTG+AGG 0.673638 tig0010964:-15505 Msa1349480:CDS
ATGAAGCCAAAGAAAGACCA+AGG 0.681190 tig0010964:-14201 Msa1349480:intron
TGTAACATTCTTCTTTGACG+GGG 0.687811 tig0010964:+14242 Msa1349480:intergenic
GTACTAACAACAGCATTGTG+AGG 0.690491 tig0010964:-13730 Msa1349480:CDS
GATAGTTCTTTGAAGATACG+AGG 0.692687 tig0010964:-13626 Msa1349480:CDS
TGTCACAACAATCTACAGGT+GGG 0.697110 tig0010964:+14586 Msa1349480:intergenic
TGAAGCGATCGAGAATGCTG+GGG 0.704654 tig0010964:-15873 Msa1349480:CDS
TTTGCTTTGCTATTTCCCTG+CGG 0.707457 tig0010964:-15900 Msa1349480:CDS
ACATACATGAGAGAGAATTG+TGG 0.732943 tig0010964:-15263 Msa1349480:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTATATATATATATATAGC+AGG + tig0010964:15580-15599 Msa1349480:intergenic 10.0%
!!! AATATACATTAAGCATTGTT+TGG - tig0010964:13726-13745 Msa1349480:CDS 20.0%
!! ATAGATATTGCAATATGTTT+GGG + tig0010964:14371-14390 Msa1349480:intergenic 20.0%
!! TTATAAGCAAACATTTCATA+AGG + tig0010964:15230-15249 Msa1349480:intergenic 20.0%
!! CTTATAAAGTTGTTGAAAAA+GGG - tig0010964:15243-15262 Msa1349480:CDS 20.0%
!! TATTCCTACTATAGATAATA+AGG - tig0010964:15298-15317 Msa1349480:CDS 20.0%
!! ATTCCTACTATAGATAATAA+GGG - tig0010964:15299-15318 Msa1349480:CDS 20.0%
!!! TCAGTTAGAAAAAAAGTATA+TGG - tig0010964:15832-15851 Msa1349480:CDS 20.0%
! ATTGTTTGGATTCAAAAGAT+TGG - tig0010964:13740-13759 Msa1349480:CDS 25.0%
!! AATTGCTTGCAAATTTCTTT+TGG + tig0010964:13997-14016 Msa1349480:intergenic 25.0%
! AAAAGAAATTTGCAAGCAAT+TGG - tig0010964:13996-14015 Msa1349480:CDS 25.0%
! TTGTTGAAGAAATTGATGAA+TGG - tig0010964:14126-14145 Msa1349480:intron 25.0%
! GAAATTGATGAATGGTAATT+CGG - tig0010964:14134-14153 Msa1349480:intron 25.0%
! TCACAAAAACAGTAATGATA+AGG - tig0010964:14167-14186 Msa1349480:intron 25.0%
! AGGAATTTAAGGAAATTCAA+TGG - tig0010964:14259-14278 Msa1349480:CDS 25.0%
! TTTAAGGAAATTCAATGGTT+AGG - tig0010964:14264-14283 Msa1349480:CDS 25.0%
! CATAGATATTGCAATATGTT+TGG + tig0010964:14372-14391 Msa1349480:intergenic 25.0%
! AACATATTGCAATATCTATG+TGG - tig0010964:14372-14391 Msa1349480:CDS 25.0%
!!! TATTTTCAAGCAAAAGTTGT+TGG - tig0010964:14636-14655 Msa1349480:CDS 25.0%
! AAGAGATTAACCCTATAATT+TGG - tig0010964:14736-14755 Msa1349480:CDS 25.0%
! TGAAAATGATTATCCTCAAA+AGG + tig0010964:14888-14907 Msa1349480:intergenic 25.0%
!! TTTGAGGATAATCATTTTCA+AGG - tig0010964:14888-14907 Msa1349480:CDS 25.0%
!! TTGAGGATAATCATTTTCAA+GGG - tig0010964:14889-14908 Msa1349480:CDS 25.0%
!! ATCAAACTCACAAGATATTT+TGG + tig0010964:14972-14991 Msa1349480:intergenic 25.0%
! TGAGTTTGATCAAAAAATGT+TGG - tig0010964:14982-15001 Msa1349480:CDS 25.0%
! GCTTATAAAGTTGTTGAAAA+AGG - tig0010964:15242-15261 Msa1349480:CDS 25.0%
!!! ATCAGAAATTGATTTTGGAT+AGG + tig0010964:15435-15454 Msa1349480:intergenic 25.0%
! ATTGTAACATTCTTCTTTGA+CGG + tig0010964:15485-15504 Msa1349480:intergenic 25.0%
! CAATAAAATTAAACTGACCT+TGG + tig0010964:15541-15560 Msa1349480:intergenic 25.0%
! TGTATTGAATTGATTGAAAC+AGG - tig0010964:15610-15629 Msa1349480:CDS 25.0%
! AGGAAATCCAATATTAACAA+AGG - tig0010964:15778-15797 Msa1349480:CDS 25.0%
! GGAAATCCAATATTAACAAA+GGG - tig0010964:15779-15798 Msa1349480:CDS 25.0%
! TTGATTCCCTTTGTTAATAT+TGG + tig0010964:15788-15807 Msa1349480:intergenic 25.0%
!! GTTAGAAAAAAAGTATATGG+AGG - tig0010964:15835-15854 Msa1349480:CDS 25.0%
!!! CTTGTTTTCAGATTTAAACT+AGG + tig0010964:15880-15899 Msa1349480:intergenic 25.0%
! TTTGTCTTTAACCTGTTAAA+TGG + tig0010964:15913-15932 Msa1349480:intergenic 25.0%
TTGAAGACAGAACTACAAAA+TGG - tig0010964:13650-13669 Msa1349480:CDS 30.0%
CAAGAAAATATGATGTGGAA+TGG - tig0010964:13923-13942 Msa1349480:CDS 30.0%
!!! ATGATCCAAAACTTTTTCAG+TGG - tig0010964:13947-13966 Msa1349480:CDS 30.0%
! AATCTCCACTGAAAAAGTTT+TGG + tig0010964:13955-13974 Msa1349480:intergenic 30.0%
!!! AAACTTTTTCAGTGGAGATT+TGG - tig0010964:13955-13974 Msa1349480:CDS 30.0%
GAGATTTGGCAAACAATATT+TGG - tig0010964:13969-13988 Msa1349480:CDS 30.0%
! ATTCTTTGAAGAAGTGAGTT+TGG + tig0010964:14085-14104 Msa1349480:intergenic 30.0%
!! ACAGTAATGATAAGGTATTG+TGG - tig0010964:14175-14194 Msa1349480:intron 30.0%
GATCCTAACAAAACACTAAT+TGG + tig0010964:14201-14220 Msa1349480:intergenic 30.0%
GTGAGAAAGTGATAACAAAT+TGG + tig0010964:14349-14368 Msa1349480:intergenic 30.0%
! GTTTCCATGGTAAATGTTTT+TGG + tig0010964:14568-14587 Msa1349480:intergenic 30.0%
GTAGGATATTACAAGGATTA+AGG + tig0010964:14590-14609 Msa1349480:intergenic 30.0%
! GCTTTTAGTAGGATATTACA+AGG + tig0010964:14597-14616 Msa1349480:intergenic 30.0%
!!! ATATGTTTTGAGTTGTTGAC+AGG - tig0010964:14855-14874 Msa1349480:CDS 30.0%
!!! TATGTTTTGAGTTGTTGACA+GGG - tig0010964:14856-14875 Msa1349480:CDS 30.0%
AGGATTGATCAAAACATACT+CGG + tig0010964:15090-15109 Msa1349480:intergenic 30.0%
CTACCCTTATTATCTATAGT+AGG + tig0010964:15305-15324 Msa1349480:intergenic 30.0%
TGCATCCATAATTAAAGATC+TGG - tig0010964:15406-15425 Msa1349480:CDS 30.0%
GGTTACCAGATCTTTAATTA+TGG + tig0010964:15414-15433 Msa1349480:intergenic 30.0%
!! GTGTCATCAGAAATTGATTT+TGG + tig0010964:15440-15459 Msa1349480:intergenic 30.0%
TTGTAACATTCTTCTTTGAC+GGG + tig0010964:15484-15503 Msa1349480:intergenic 30.0%
!! GATTTTGTTGCTTGCAATTT+AGG + tig0010964:15640-15659 Msa1349480:intergenic 30.0%
TTAACCTGTTAAATGGTGAT+TGG + tig0010964:15906-15925 Msa1349480:intergenic 30.0%
! AACTATCCATATCTGAAACA+TGG + tig0010964:16083-16102 Msa1349480:intergenic 30.0%
TTCTTACATAAGGACTCAAA+GGG + tig0010964:16212-16231 Msa1349480:intergenic 30.0%
CCATATGTTGTTCTTACATA+AGG + tig0010964:16222-16241 Msa1349480:intergenic 30.0%
CCTTATGTAAGAACAACATA+TGG - tig0010964:16219-16238 Msa1349480:CDS 30.0%
! TTGGATTCAAAAGATTGGTC+TGG - tig0010964:13745-13764 Msa1349480:CDS 35.0%
!! TGGATTCAAAAGATTGGTCT+GGG - tig0010964:13746-13765 Msa1349480:CDS 35.0%
GCTTGCAAGAAAATATGATG+TGG - tig0010964:13918-13937 Msa1349480:CDS 35.0%
!! TGGCCAATTAGTGTTTTGTT+AGG - tig0010964:14195-14214 Msa1349480:intron 35.0%
! GAAAGAGTTTTCTCTTGTGA+CGG - tig0010964:14413-14432 Msa1349480:CDS 35.0%
!! AGTTGATGAACAAGGATTTG+TGG - tig0010964:14436-14455 Msa1349480:CDS 35.0%
ACATACATGAGAGAGAATTG+TGG - tig0010964:14459-14478 Msa1349480:CDS 35.0%
! GATTTTGTTCTCTATACCTG+TGG - tig0010964:14494-14513 Msa1349480:CDS 35.0%
!!! CATGGTAAATGTTTTTGGAG+TGG + tig0010964:14563-14582 Msa1349480:intergenic 35.0%
CACTCCAAAAACATTTACCA+TGG - tig0010964:14561-14580 Msa1349480:CDS 35.0%
TACAAGGATTAAGGTTTCCA+TGG + tig0010964:14581-14600 Msa1349480:intergenic 35.0%
!!! GTGAGTTTCTAGCTTTTAGT+AGG + tig0010964:14608-14627 Msa1349480:intergenic 35.0%
AAAGCTAGAAACTCACAAGA+AGG - tig0010964:14612-14631 Msa1349480:CDS 35.0%
CAAGCAAAAGTTGTTGGATT+TGG - tig0010964:14642-14661 Msa1349480:CDS 35.0%
GGATTGTCATCTGTTGAAAA+TGG - tig0010964:14663-14682 Msa1349480:CDS 35.0%
! AATTGGATTATGTGTTGGTG+AGG + tig0010964:14712-14731 Msa1349480:intergenic 35.0%
TCACCAACACATAATCCAAT+TGG - tig0010964:14711-14730 Msa1349480:CDS 35.0%
TCACCAATTGGATTATGTGT+TGG + tig0010964:14717-14736 Msa1349480:intergenic 35.0%
CAGGTGCATACCAAATTATA+GGG + tig0010964:14749-14768 Msa1349480:intergenic 35.0%
TCAGGTGCATACCAAATTAT+AGG + tig0010964:14750-14769 Msa1349480:intergenic 35.0%
TATAATTTGGTATGCACCTG+AGG - tig0010964:14749-14768 Msa1349480:CDS 35.0%
TTTCTCTGAGTACTTACAAG+AGG + tig0010964:14811-14830 Msa1349480:intergenic 35.0%
CATACCAAAGCTATATGCAT+CGG + tig0010964:14835-14854 Msa1349480:intergenic 35.0%
ACCAACCAAAATATCAAAGC+AGG - tig0010964:14918-14937 Msa1349480:CDS 35.0%
!!! TCCTGCTTTGATATTTTGGT+TGG + tig0010964:14922-14941 Msa1349480:intergenic 35.0%
! TCTCTCCTGCTTTGATATTT+TGG + tig0010964:14926-14945 Msa1349480:intergenic 35.0%
AAAATATCAAAGCAGGAGAG+AGG - tig0010964:14925-14944 Msa1349480:CDS 35.0%
CTACAAATAGATGAGAAACC+AGG + tig0010964:15029-15048 Msa1349480:intergenic 35.0%
CTGGTTTCTCATCTATTTGT+AGG - tig0010964:15027-15046 Msa1349480:CDS 35.0%
! AGTTCAAGCTGATTTAGTTC+AGG + tig0010964:15110-15129 Msa1349480:intergenic 35.0%
TCAATGTCACAACAATCTAC+AGG + tig0010964:15143-15162 Msa1349480:intergenic 35.0%
GTAGATTGTTGTGACATTGA+AGG - tig0010964:15143-15162 Msa1349480:CDS 35.0%
! TTTTCTCAAGAGTTTTCCTG+TGG - tig0010964:15169-15188 Msa1349480:CDS 35.0%
ATATTATATGTCCTCTCCAC+AGG + tig0010964:15188-15207 Msa1349480:intergenic 35.0%
TGTAACATTCTTCTTTGACG+GGG + tig0010964:15483-15502 Msa1349480:intergenic 35.0%
! TTCTTTGGCTTCATTTGTGA+TGG + tig0010964:15515-15534 Msa1349480:intergenic 35.0%
TCATTACCACCAACATTATC+TGG - tig0010964:15658-15677 Msa1349480:CDS 35.0%
AACTTGATGTCAATGATGAC+TGG + tig0010964:15711-15730 Msa1349480:intergenic 35.0%
CTATAACCCCAGTTAAAAGA+AGG - tig0010964:15731-15750 Msa1349480:CDS 35.0%
! TTGACACGTCTTATTCTTGT+TGG + tig0010964:15970-15989 Msa1349480:intergenic 35.0%
! GATAGTTCTTTGAAGATACG+AGG - tig0010964:16096-16115 Msa1349480:CDS 35.0%
GTTCTTACATAAGGACTCAA+AGG + tig0010964:16213-16232 Msa1349480:intergenic 35.0%
!! TGGAAGTCTAAAAGCGCTTA+TGG - tig0010964:13519-13538 Msa1349480:CDS 40.0%
ATGCTAGAGTGAATATGTCG+TGG + tig0010964:13591-13610 Msa1349480:intergenic 40.0%
ACACAATCGCAGATGAGATA+AGG - tig0010964:13614-13633 Msa1349480:CDS 40.0%
! TCTCTAAGAGGACATTCAAG+AGG + tig0010964:13679-13698 Msa1349480:intergenic 40.0%
! CTTTCCAGGGTATTCAAAGA+AGG - tig0010964:13700-13719 Msa1349480:CDS 40.0%
TTCACCTTCTTTGAATACCC+TGG + tig0010964:13707-13726 Msa1349480:intergenic 40.0%
!! TTTGCTTTGCTATTTCCCTG+CGG - tig0010964:13822-13841 Msa1349480:CDS 40.0%
AGAATGCTTGGAGAGAAGAT+AGG - tig0010964:14019-14038 Msa1349480:CDS 40.0%
! GAAGCACTCAAGGAGAAAAA+AGG - tig0010964:14054-14073 Msa1349480:CDS 40.0%
GATCCAAAGATTACCAAGTG+AGG - tig0010964:14217-14236 Msa1349480:CDS 40.0%
! TCTCCTCACTTGGTAATCTT+TGG + tig0010964:14223-14242 Msa1349480:intergenic 40.0%
TTACCAAGTGAGGAGAAGAT+TGG - tig0010964:14227-14246 Msa1349480:CDS 40.0%
TACCAAGTGAGGAGAAGATT+GGG - tig0010964:14228-14247 Msa1349480:CDS 40.0%
! TAGGACAAAGTTTTGCTCTG+AGG - tig0010964:14283-14302 Msa1349480:CDS 40.0%
!!! TTTGCTCTGAGGCATTTTCT+TGG - tig0010964:14294-14313 Msa1349480:CDS 40.0%
!!! TTGCTCTGAGGCATTTTCTT+GGG - tig0010964:14295-14314 Msa1349480:CDS 40.0%
GAGAACAAAATCTGTCTCCT+TGG + tig0010964:14486-14505 Msa1349480:intergenic 40.0%
TTTGTTCTCTATACCTGTGG+TGG - tig0010964:14497-14516 Msa1349480:CDS 40.0%
GGTTGATCTCATGCTTCAAA+TGG - tig0010964:14518-14537 Msa1349480:CDS 40.0%
GGGTTAATCTCTTCACCAAT+TGG + tig0010964:14729-14748 Msa1349480:intergenic 40.0%
! TCTAGCTCAGTTAAAACCTC+AGG + tig0010964:14768-14787 Msa1349480:intergenic 40.0%
AAAGCCGATGCATATAGCTT+TGG - tig0010964:14828-14847 Msa1349480:CDS 40.0%
! GACAGGGAAAATTCCTTTTG+AGG - tig0010964:14872-14891 Msa1349480:CDS 40.0%
!!! AGATATTTTGGCGAGCGATA+TGG + tig0010964:14960-14979 Msa1349480:intergenic 40.0%
TGTCACAACAATCTACAGGT+GGG + tig0010964:15139-15158 Msa1349480:intergenic 40.0%
ATGTCACAACAATCTACAGG+TGG + tig0010964:15140-15159 Msa1349480:intergenic 40.0%
CATCTTCACAAACTGTGCTT+TGG + tig0010964:15382-15401 Msa1349480:intergenic 40.0%
ATGAAGCCAAAGAAAGACCA+AGG - tig0010964:15521-15540 Msa1349480:CDS 40.0%
! AACTGACCTTGGTCTTTCTT+TGG + tig0010964:15530-15549 Msa1349480:intergenic 40.0%
TACCACCAACATTATCTGGT+CGG - tig0010964:15662-15681 Msa1349480:CDS 40.0%
TCGGGTTTCAAGGACAAATA+AGG - tig0010964:15681-15700 Msa1349480:CDS 40.0%
!! TTGGAGGCCTTCTTTTAACT+GGG + tig0010964:15741-15760 Msa1349480:intergenic 40.0%
! TTTGGAGGCCTTCTTTTAAC+TGG + tig0010964:15742-15761 Msa1349480:intergenic 40.0%
!!! TCAGATTCTGAGACTTTTGG+AGG + tig0010964:15757-15776 Msa1349480:intergenic 40.0%
!!! CTCTCAGATTCTGAGACTTT+TGG + tig0010964:15760-15779 Msa1349480:intergenic 40.0%
CAAAAGTCTCAGAATCTGAG+AGG - tig0010964:15758-15777 Msa1349480:CDS 40.0%
GAGACCAATCACCATTTAAC+AGG - tig0010964:15899-15918 Msa1349480:CDS 40.0%
GTACTAACAACAGCATTGTG+AGG - tig0010964:15992-16011 Msa1349480:CDS 40.0%
AGCATTGTGAGGATGCAAAA+AGG - tig0010964:16003-16022 Msa1349480:CDS 40.0%
GCATTGTGAGGATGCAAAAA+GGG - tig0010964:16004-16023 Msa1349480:CDS 40.0%
TATGACCTTGAAGGACTCAT+TGG + tig0010964:16045-16064 Msa1349480:intergenic 40.0%
ATGCTGCCATGTTTCAGATA+TGG - tig0010964:16074-16093 Msa1349480:CDS 40.0%
CTCAAAGGACTCAATGGAAA+TGG + tig0010964:16132-16151 Msa1349480:intergenic 40.0%
TATGGACTCAAAGGACTCAA+TGG + tig0010964:16138-16157 Msa1349480:intergenic 40.0%
TTGCTCACATATGGACTCAA+AGG + tig0010964:16147-16166 Msa1349480:intergenic 40.0%
GTCCATATGTGAGCAATCAA+AGG - tig0010964:16151-16170 Msa1349480:CDS 40.0%
GTCCTTTGATTGCTCACATA+TGG + tig0010964:16156-16175 Msa1349480:intergenic 40.0%
AGCAATCAAAGGACTAGAAG+AGG - tig0010964:16162-16181 Msa1349480:CDS 40.0%
TCCTCTTAGAGAGCTTTCCA+GGG - tig0010964:13687-13706 Msa1349480:CDS 45.0%
ATTGAAGCGATCGAGAATGC+TGG - tig0010964:13847-13866 Msa1349480:CDS 45.0%
TTGAAGCGATCGAGAATGCT+GGG - tig0010964:13848-13867 Msa1349480:CDS 45.0%
GCAAGCAATTGGAGAATGCT+TGG - tig0010964:14007-14026 Msa1349480:CDS 45.0%
ATGCTTGGAGAGAAGATAGG+TGG - tig0010964:14022-14041 Msa1349480:CDS 45.0%
TTCCCAATCTTCTCCTCACT+TGG + tig0010964:14233-14252 Msa1349480:intergenic 45.0%
AAGTGAGGAGAAGATTGGGA+AGG - tig0010964:14232-14251 Msa1349480:CDS 45.0%
AGTGAGGAGAAGATTGGGAA+GGG - tig0010964:14233-14252 Msa1349480:CDS 45.0%
!!! TGCTCTGAGGCATTTTCTTG+GGG - tig0010964:14296-14315 Msa1349480:CDS 45.0%
!! TGTGACGGAGTTGATGAACA+AGG - tig0010964:14428-14447 Msa1349480:CDS 45.0%
TGAGAGAGAATTGTGGTCCA+AGG - tig0010964:14466-14485 Msa1349480:CDS 45.0%
ATCTCATGCTTCAAATGGCC+AGG - tig0010964:14523-14542 Msa1349480:CDS 45.0%
TCTCATGCTTCAAATGGCCA+GGG - tig0010964:14524-14543 Msa1349480:CDS 45.0%
! TCAAGAGTTTTCCTGTGGAG+AGG - tig0010964:15174-15193 Msa1349480:CDS 45.0%
TAGTGAGTCTGAGCCAACAA+AGG - tig0010964:15322-15341 Msa1349480:CDS 45.0%
ACAGTAGCCACATCCTTTGT+TGG + tig0010964:15338-15357 Msa1349480:intergenic 45.0%
ACCACCAACATTATCTGGTC+GGG - tig0010964:15663-15682 Msa1349480:CDS 45.0%
GAAACCCGACCAGATAATGT+TGG + tig0010964:15670-15689 Msa1349480:intergenic 45.0%
CATTATCTGGTCGGGTTTCA+AGG - tig0010964:15671-15690 Msa1349480:CDS 45.0%
!! TGGAGGCCTTCTTTTAACTG+GGG + tig0010964:15740-15759 Msa1349480:intergenic 45.0%
CTTCTGGTGTATGACCTTGA+AGG + tig0010964:16054-16073 Msa1349480:intergenic 45.0%
TGAAACATGGCAGCATCTTC+TGG + tig0010964:16070-16089 Msa1349480:intergenic 45.0%
CTCAAAGGGCTCAATGCTAA+TGG + tig0010964:16198-16217 Msa1349480:intergenic 45.0%
CAAGAACCTCACCAATGTGC+CGG + tig0010964:13501-13520 Msa1349480:intergenic 50.0%
GTCCTCTTAGAGAGCTTTCC+AGG - tig0010964:13686-13705 Msa1349480:CDS 50.0%
ACCCTGGAAAGCTCTCTAAG+AGG + tig0010964:13691-13710 Msa1349480:intergenic 50.0%
CGATCGCTTCAATAACCGCA+GGG + tig0010964:13840-13859 Msa1349480:intergenic 50.0%
TCGATCGCTTCAATAACCGC+AGG + tig0010964:13841-13860 Msa1349480:intergenic 50.0%
TGAAGCGATCGAGAATGCTG+GGG - tig0010964:13849-13868 Msa1349480:CDS 50.0%
AGAATGCTGGGGAGATGTCA+GGG - tig0010964:13860-13879 Msa1349480:CDS 50.0%
CTTGGAGAGAAGATAGGTGG+AGG - tig0010964:14025-14044 Msa1349480:CDS 50.0%
!! GAGGCTCATAGAAGCACTCA+AGG - tig0010964:14044-14063 Msa1349480:CDS 50.0%
GTGAGGAGAAGATTGGGAAG+GGG - tig0010964:14234-14253 Msa1349480:CDS 50.0%
TGAGGAGAAGATTGGGAAGG+GGG - tig0010964:14235-14254 Msa1349480:CDS 50.0%
GGGAAGGGGGAAGGAATTTA+AGG - tig0010964:14248-14267 Msa1349480:CDS 50.0%
AGCATGAGATCAACCACCAC+AGG + tig0010964:14513-14532 Msa1349480:intergenic 50.0%
CTCATGCTTCAAATGGCCAG+GGG - tig0010964:14525-14544 Msa1349480:CDS 50.0%
GTGGAGATATTCAATGCCCC+TGG + tig0010964:14544-14563 Msa1349480:intergenic 50.0%
GGCGAGCGATATGGAAACAA+AGG + tig0010964:14951-14970 Msa1349480:intergenic 50.0%
GTCTGAGCCAACAAAGGATG+TGG - tig0010964:15328-15347 Msa1349480:CDS 50.0%
ACCCGACCAGATAATGTTGG+TGG + tig0010964:15667-15686 Msa1349480:intergenic 50.0%
GACCTTGAAGGACTCATTGG+CGG + tig0010964:16042-16061 Msa1349480:intergenic 50.0%
ATGGAACAGCTCCGGCACAT+TGG - tig0010964:13487-13506 Msa1349480:CDS 55.0%
CGGCACATTGGTGAGGTTCT+TGG - tig0010964:13499-13518 Msa1349480:CDS 55.0%
GAGAATGCTGGGGAGATGTC+AGG - tig0010964:13859-13878 Msa1349480:CDS 55.0%
GGAGATGTCAGGGTTGGATC+AGG - tig0010964:13870-13889 Msa1349480:CDS 55.0%
GAGAAGATTGGGAAGGGGGA+AGG - tig0010964:14239-14258 Msa1349480:CDS 55.0%
GAGAAACCAGGACGCTGAGA+CGG + tig0010964:15017-15036 Msa1349480:intergenic 55.0%
CACCGCCAATGAGTCCTTCA+AGG - tig0010964:16037-16056 Msa1349480:CDS 55.0%
ACAGCTCCGGCACATTGGTG+AGG - tig0010964:13492-13511 Msa1349480:CDS 60.0%
TGCTGGGGAGATGTCAGGGT+TGG - tig0010964:13864-13883 Msa1349480:CDS 60.0%
ACTGATCCGTCTCAGCGTCC+TGG - tig0010964:15008-15027 Msa1349480:CDS 60.0%
Chromosome Type Strat End Strand Name
tig0010964 gene 13487 16257 13487 ID=Msa1349480;Name=Msa1349480
tig0010964 mRNA 13487 16257 13487 ID=Msa1349480-mRNA-1;Parent=Msa1349480;Name=Msa1349480-mRNA-1;_AED=0.01;_eAED=0.01;_QI=0|0|0|1|1|1|2|0|893
tig0010964 exon 14202 16257 14202 ID=Msa1349480-mRNA-1:exon:6940;Parent=Msa1349480-mRNA-1
tig0010964 exon 13487 14112 13487 ID=Msa1349480-mRNA-1:exon:6939;Parent=Msa1349480-mRNA-1
tig0010964 CDS 14202 16257 14202 ID=Msa1349480-mRNA-1:cds;Parent=Msa1349480-mRNA-1
tig0010964 CDS 13487 14112 13487 ID=Msa1349480-mRNA-1:cds;Parent=Msa1349480-mRNA-1
Gene Sequence

>Msa1349480

ATGGAACAGCTCCGGCACATTGGTGAGGTTCTTGGAAGTCTAAAAGCGCTTATGGTTTTAAGCGACGAAATTCAAATCAATCAACGACAATGTTGTTTGCTCCACGACATATTCACTCTAGCATTCGACACAATCGCAGATGAGATAAGGCAGAATCTGAAGCTTGAAGACAGAACTACAAAATGGAAGCCTCTTGAATGTCCTCTTAGAGAGCTTTCCAGGGTATTCAAAGAAGGTGAAATATACATTAAGCATTGTTTGGATTCAAAAGATTGGTCTGGGAAAGCACTCACACTTTCTCAGAACAGAGAATGTGTTGAGTTTCACATTCATAATTTGCTTTGCTATTTCCCTGCGGTTATTGAAGCGATCGAGAATGCTGGGGAGATGTCAGGGTTGGATCAGGATGAGAATTCGAAGAAGAAATTGATGCTTGCAAGAAAATATGATGTGGAATGGAATGATCCAAAACTTTTTCAGTGGAGATTTGGCAAACAATATTTGGTTCCAAAAGAAATTTGCAAGCAATTGGAGAATGCTTGGAGAGAAGATAGGTGGAGGCTCATAGAAGCACTCAAGGAGAAAAAAGGTTCTTCCAAACTCACTTCTTCAAAGAATGATCAACATTTTGCAGATATGTTGTTGAAGAAATTGATGAATGGTAATTCGGATAAAACTAATCACAAAAACAGTAATGATAAGGTATTGTGGCCAATTAGTGTTTTGTTAGGATCCAAAGATTACCAAGTGAGGAGAAGATTGGGAAGGGGGAAGGAATTTAAGGAAATTCAATGGTTAGGACAAAGTTTTGCTCTGAGGCATTTTCTTGGGGAGAGAGAAGCTTATGAAAATGAGATTTCCAATTTGTTATCACTTTCTCACCCAAACATATTGCAATATCTATGTGGTTTTTATGATGAAGAGAAGAAAGAGTTTTCTCTTGTGACGGAGTTGATGAACAAGGATTTGTGGACATACATGAGAGAGAATTGTGGTCCAAGGAGACAGATTTTGTTCTCTATACCTGTGGTGGTTGATCTCATGCTTCAAATGGCCAGGGGCATTGAATATCTCCACTCCAAAAACATTTACCATGGAAACCTTAATCCTTGTAATATCCTACTAAAAGCTAGAAACTCACAAGAAGGTTATTTTCAAGCAAAAGTTGTTGGATTTGGATTGTCATCTGTTGAAAATGGTGAAATTTATAATGCTTCTAGATCCTCACCAACACATAATCCAATTGGTGAAGAGATTAACCCTATAATTTGGTATGCACCTGAGGTTTTAACTGAGCTAGAACAAATGAAAAATGCTTCTACCTCTTGTAAGTACTCAGAGAAAGCCGATGCATATAGCTTTGGTATGATATGTTTTGAGTTGTTGACAGGGAAAATTCCTTTTGAGGATAATCATTTTCAAGGGATTAGAACCAACCAAAATATCAAAGCAGGAGAGAGGCCTTTGTTTCCATATCGCTCGCCAAAATATCTTGTGAGTTTGATCAAAAAATGTTGGCAAACTGATCCGTCTCAGCGTCCTGGTTTCTCATCTATTTGTAGGATTTTGCGCTACATCAAAAAGTTTCTTTCAATGAACACCGAGTATGTTTTGATCAATCCTGAACTAAATCAGCTTGAACTTCAGAACCCACCTGTAGATTGTTGTGACATTGAAGGAATTTTTCTCAAGAGTTTTCCTGTGGAGAGGACATATAATATGTCTTGTGTTTCACAAATACCTTATGAAATGTTTGCTTATAAAGTTGTTGAAAAAGGGAAGATTAATAATCAGAATCTGAGTAATAATACTATTCCTACTATAGATAATAAGGGTAGTGAGTCTGAGCCAACAAAGGATGTGGCTACTGTTCGTAGCGAAGAAAGTGATGACCAAAGCACAGTTTGTGAAGATGTTGATGCATCCATAATTAAAGATCTGGTAACCTATCCAAAATCAATTTCTGATGACACACAGTCTATTTTTTATGATACCCCGTCAAAGAAGAATGTTACAATAAAGAAACCATCACAAATGAAGCCAAAGAAAGACCAAGGAACGCCTAAATTGCAAGCAACAAAATCATTACCACCAACATTATCTGGTCGGGTTTCAAGGACAAATAAGGCGAACCAGTCATCATTGACATCAAGTTCTATAACCCCAGTTAAAAGAAGGCCTCCAAAAGTCTCAGAATCTGAGAGGAAATCCAATATTAACAAAGGGAATCAATCAGCATCAGTGTCAACTTCAAGCTCAGTTAGAAAAAAAGTATATGGAGGCAATGTCTCAGATTCTAAGCCTAGTTTAAATCTGAAAACAAGAGACCAATCACCATTTAACAGGTTAAAGACAAAAAAATCACAACTAACGATGAATTCTTCTTCGAGTCCAACAAGAATAAGACGTGTCAATAGTACTAACAACAGCATTGTGAGGATGCAAAAAGGGTTCATTTCAACACCGCCAATGAGTCCTTCAAGGTCATACACCAGAAGATGCTGCCATGTTTCAGATATGGATAGTTCTTTGAAGATACGAGGTAGATTGTCACCATTTCCATTGAGTCCTTTGAGTCCATATGTGAGCAATCAAAGGACTAGAAGAGGAAGCTTGTCACCATTAGCATTGAGCCCTTTGAGTCCTTATGTAAGAACAACATATGGTCATGTCTCAGATTAA
Protein sequence

>Msa1349480

MEQLRHIGEVLGSLKALMVLSDEIQINQRQCCLLHDIFTLAFDTIADEIRQNLKLEDRTTKWKPLECPLRELSRVFKEGEIYIKHCLDSKDWSGKALTLSQNRECVEFHIHNLLCYFPAVIEAIENAGEMSGLDQDENSKKKLMLARKYDVEWNDPKLFQWRFGKQYLVPKEICKQLENAWREDRWRLIEALKEKKGSSKLTSSKNDQHFADMLLKKLMNGNSDKTNHKNSNDKVLWPISVLLGSKDYQVRRRLGRGKEFKEIQWLGQSFALRHFLGEREAYENEISNLLSLSHPNILQYLCGFYDEEKKEFSLVTELMNKDLWTYMRENCGPRRQILFSIPVVVDLMLQMARGIEYLHSKNIYHGNLNPCNILLKARNSQEGYFQAKVVGFGLSSVENGEIYNASRSSPTHNPIGEEINPIIWYAPEVLTELEQMKNASTSCKYSEKADAYSFGMICFELLTGKIPFEDNHFQGIRTNQNIKAGERPLFPYRSPKYLVSLIKKCWQTDPSQRPGFSSICRILRYIKKFLSMNTEYVLINPELNQLELQNPPVDCCDIEGIFLKSFPVERTYNMSCVSQIPYEMFAYKVVEKGKINNQNLSNNTIPTIDNKGSESEPTKDVATVRSEESDDQSTVCEDVDASIIKDLVTYPKSISDDTQSIFYDTPSKKNVTIKKPSQMKPKKDQGTPKLQATKSLPPTLSGRVSRTNKANQSSLTSSSITPVKRRPPKVSESERKSNINKGNQSASVSTSSSVRKKVYGGNVSDSKPSLNLKTRDQSPFNRLKTKKSQLTMNSSSSPTRIRRVNSTNNSIVRMQKGFISTPPMSPSRSYTRRCCHVSDMDSSLKIRGRLSPFPLSPLSPYVSNQRTRRGSLSPLALSPLSPYVRTTYGHVSD*