AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1351330


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1351330 MtrunA17_Chr2g0296781 96.512 172 6 0 41 212 1 172 2.87e-116 347
Msa1351330 MtrunA17_Chr1g0196541 58.140 172 72 0 41 212 1 172 3.11e-63 209
Msa1351330 MtrunA17_Chr6g0476431 94.318 88 5 0 41 128 1 88 2.66e-56 174
Msa1351330 MtrunA17_Chr6g0477261 93.333 60 4 0 152 211 1 60 2.07e-30 117
Msa1351330 MtrunA17_Chr6g0451441 31.746 189 119 5 17 199 64 248 1.43e-23 97.8
Msa1351330 MtrunA17_Chr5g0441581 32.961 179 107 4 37 212 32 200 9.28e-20 87.0
Msa1351330 MtrunA17_Chr5g0448761 27.660 188 126 5 37 215 60 246 7.22e-14 70.1
Msa1351330 MtrunA17_Chr2g0329031 28.177 181 115 5 35 210 12 182 7.01e-13 67.0
Msa1351330 MtrunA17_Chr2g0291591 24.870 193 131 3 27 212 54 239 7.55e-12 63.9
Msa1351330 MtrunA17_Chr7g0258861 28.000 175 121 3 37 206 10 184 1.88e-11 62.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1351330 AT1G76320.2 32.222 180 106 5 41 214 1 170 3.36e-17 80.1
Msa1351330 AT1G76320.1 32.222 180 106 5 41 214 1 170 3.99e-17 80.1
Msa1351330 AT1G76320.4 32.222 180 106 5 41 214 1 170 3.99e-17 80.1
Msa1351330 AT1G76320.3 32.222 180 106 5 41 214 1 170 4.12e-17 79.7
Msa1351330 AT4G38180.1 26.316 171 118 3 37 199 73 243 2.67e-12 65.9
Msa1351330 AT4G15090.1 23.370 184 136 3 37 215 51 234 4.41e-11 62.4
Msa1351330 AT4G15090.5 23.370 184 136 3 37 215 51 234 4.41e-11 62.4
Msa1351330 AT4G15090.3 23.370 184 136 3 37 215 51 234 4.41e-11 62.4
Msa1351330 AT4G15090.2 23.370 184 136 3 37 215 51 234 4.41e-11 62.4
Msa1351330 AT4G15090.4 23.370 184 136 3 37 215 51 234 4.41e-11 62.4

Find 37 sgRNAs with CRISPR-Local

Find 116 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTACACTGCGTTTGAGTTAT+AGG 0.177962 tig0012201:-51402 None:intergenic
AATGCGTGTATTTATTTAGA+TGG 0.249459 tig0012201:+51435 Msa1351330:CDS
TATGCTGAACGGGTGGGTTT+TGG 0.279098 tig0012201:+51267 Msa1351330:CDS
TTAGATCGAACAAGAATATA+GGG 0.302032 tig0012201:+51526 Msa1351330:CDS
TGCGTTTGAGTTATAGGATT+TGG 0.304891 tig0012201:-51396 None:intergenic
ATTTGAGTCTATTGATGAAT+TGG 0.319207 tig0012201:+51140 Msa1351330:CDS
GGTTATGAAAATCTCACATT+TGG 0.334024 tig0012201:+51636 Msa1351330:CDS
TTTAGATCGAACAAGAATAT+AGG 0.366556 tig0012201:+51525 Msa1351330:CDS
CTAAAATACCTTGCTTTCCC+TGG 0.410842 tig0012201:-51507 None:intergenic
TCCTTTGTCGTTGAAGCAAA+TGG 0.422234 tig0012201:+51615 Msa1351330:CDS
CCTTGTGATGGGATGTCATT+CGG 0.449339 tig0012201:+51210 Msa1351330:CDS
CATAATCATGATCTAAGTCC+AGG 0.457184 tig0012201:+51489 Msa1351330:CDS
TTACAAGAATTATGCTGAAC+GGG 0.461474 tig0012201:+51257 Msa1351330:CDS
AAGCAAGACGACTACGACTT+GGG 0.474410 tig0012201:+51688 Msa1351330:CDS
TATAACTCAAACGCAGTGTA+AGG 0.477984 tig0012201:+51404 Msa1351330:CDS
AATGATTGTTTAGAAGCAAA+TGG 0.480295 tig0012201:+51168 Msa1351330:CDS
GATGTCATTCGGATCTGAAA+AGG 0.494917 tig0012201:+51221 Msa1351330:CDS
TTTGTTGTATTAGTGCAAGA+AGG 0.500028 tig0012201:+51117 Msa1351330:intron
AAAGCAAGACGACTACGACT+TGG 0.536463 tig0012201:+51687 Msa1351330:CDS
ATCTAAGTCCAGGGAAAGCA+AGG 0.538270 tig0012201:+51499 Msa1351330:CDS
AAGAATTATGCTGAACGGGT+GGG 0.547191 tig0012201:+51261 Msa1351330:CDS
AGCATAATTCTTGTAATACT+CGG 0.547475 tig0012201:-51250 None:intergenic
ACCATTTGCTTCAACGACAA+AGG 0.554941 tig0012201:-51616 None:intergenic
CAAGAATTATGCTGAACGGG+TGG 0.555569 tig0012201:+51260 Msa1351330:CDS
TTAGAACTCAATGATCAAGC+TGG 0.558672 tig0012201:+51567 Msa1351330:CDS
TTGCTTCAACGACAAAGGAT+CGG 0.564208 tig0012201:-51611 None:intergenic
AGACGACTACGACTTGGGAC+AGG 0.570488 tig0012201:+51693 Msa1351330:CDS
TAGAACTCAATGATCAAGCT+GGG 0.577189 tig0012201:+51568 Msa1351330:CDS
ATGTACTCAAGTTCAAGAAG+AGG 0.581014 tig0012201:+51736 Msa1351330:CDS
ATTACAAGAATTATGCTGAA+CGG 0.604383 tig0012201:+51256 Msa1351330:CDS
AAGAAGAAACACCTTGTGAT+GGG 0.621474 tig0012201:+51199 Msa1351330:CDS
ATATAGGGGCACAAATGAAG+AGG 0.627813 tig0012201:+51541 Msa1351330:CDS
GAAGAAGAAACACCTTGTGA+TGG 0.629834 tig0012201:+51198 Msa1351330:CDS
TAGATCGAACAAGAATATAG+GGG 0.631587 tig0012201:+51527 Msa1351330:CDS
CCGAATGACATCCCATCACA+AGG 0.631658 tig0012201:-51210 None:intergenic
TAGGGGCACAAATGAAGAGG+AGG 0.634884 tig0012201:+51544 Msa1351330:CDS
ATAATCATGATCTAAGTCCA+GGG 0.638934 tig0012201:+51490 Msa1351330:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAAAATAAGTTCAAAAAA+AGG + tig0012201:51294-51313 Msa1351330:CDS 10.0%
!! AAAAAAATAAGTTCAAAAAA+AGG + tig0012201:51294-51313 Msa1351330:CDS 10.0%
!! TAAATGAAAAATCAATCAAT+CGG - tig0012201:51056-51075 None:intergenic 15.0%
!! TAAATGAAAAATCAATCAAT+CGG - tig0012201:51056-51075 None:intergenic 15.0%
!!! TGTTTTTATAGATCATTAGT+AGG + tig0012201:51021-51040 Msa1351330:intron 20.0%
!! CTGTGTTAGTTAATTAATAT+AGG + tig0012201:51081-51100 Msa1351330:intron 20.0%
!!! TGTTTTTATAGATCATTAGT+AGG + tig0012201:51021-51040 Msa1351330:intron 20.0%
!! CTGTGTTAGTTAATTAATAT+AGG + tig0012201:51081-51100 Msa1351330:intron 20.0%
! ATTTGAGTCTATTGATGAAT+TGG + tig0012201:51140-51159 Msa1351330:CDS 25.0%
! AATGATTGTTTAGAAGCAAA+TGG + tig0012201:51168-51187 Msa1351330:CDS 25.0%
! AGCATAATTCTTGTAATACT+CGG - tig0012201:51253-51272 None:intergenic 25.0%
! ATTACAAGAATTATGCTGAA+CGG + tig0012201:51256-51275 Msa1351330:CDS 25.0%
! AATGCGTGTATTTATTTAGA+TGG + tig0012201:51435-51454 Msa1351330:CDS 25.0%
! TTTAGATCGAACAAGAATAT+AGG + tig0012201:51525-51544 Msa1351330:CDS 25.0%
! TTAGATCGAACAAGAATATA+GGG + tig0012201:51526-51545 Msa1351330:CDS 25.0%
! ATTTGAGTCTATTGATGAAT+TGG + tig0012201:51140-51159 Msa1351330:CDS 25.0%
! AATGATTGTTTAGAAGCAAA+TGG + tig0012201:51168-51187 Msa1351330:CDS 25.0%
! AGCATAATTCTTGTAATACT+CGG - tig0012201:51253-51272 None:intergenic 25.0%
! ATTACAAGAATTATGCTGAA+CGG + tig0012201:51256-51275 Msa1351330:CDS 25.0%
! AATGCGTGTATTTATTTAGA+TGG + tig0012201:51435-51454 Msa1351330:CDS 25.0%
! TTTAGATCGAACAAGAATAT+AGG + tig0012201:51525-51544 Msa1351330:CDS 25.0%
! TTAGATCGAACAAGAATATA+GGG + tig0012201:51526-51545 Msa1351330:CDS 25.0%
! TTTGTTGTATTAGTGCAAGA+AGG + tig0012201:51117-51136 Msa1351330:intron 30.0%
TTACAAGAATTATGCTGAAC+GGG + tig0012201:51257-51276 Msa1351330:CDS 30.0%
AAGTTCAAAAAAAGGAGATG+AGG + tig0012201:51302-51321 Msa1351330:CDS 30.0%
AGTTCAAAAAAAGGAGATGA+GGG + tig0012201:51303-51322 Msa1351330:CDS 30.0%
ATAATCATGATCTAAGTCCA+GGG + tig0012201:51490-51509 Msa1351330:CDS 30.0%
TAGATCGAACAAGAATATAG+GGG + tig0012201:51527-51546 Msa1351330:CDS 30.0%
GGTTATGAAAATCTCACATT+TGG + tig0012201:51636-51655 Msa1351330:CDS 30.0%
! TTTGTTGTATTAGTGCAAGA+AGG + tig0012201:51117-51136 Msa1351330:intron 30.0%
TTACAAGAATTATGCTGAAC+GGG + tig0012201:51257-51276 Msa1351330:CDS 30.0%
AAGTTCAAAAAAAGGAGATG+AGG + tig0012201:51302-51321 Msa1351330:CDS 30.0%
AGTTCAAAAAAAGGAGATGA+GGG + tig0012201:51303-51322 Msa1351330:CDS 30.0%
ATAATCATGATCTAAGTCCA+GGG + tig0012201:51490-51509 Msa1351330:CDS 30.0%
TAGATCGAACAAGAATATAG+GGG + tig0012201:51527-51546 Msa1351330:CDS 30.0%
GGTTATGAAAATCTCACATT+TGG + tig0012201:51636-51655 Msa1351330:CDS 30.0%
TGATACCCTTCACAAAAACA+AGG + tig0012201:50741-50760 Msa1351330:CDS 35.0%
! TCAATCAGTCTTTGATTCGT+GGG - tig0012201:50804-50823 None:intergenic 35.0%
! TTCAATCAGTCTTTGATTCG+TGG - tig0012201:50805-50824 None:intergenic 35.0%
ATATGAGATCTGATTCACAG+AGG - tig0012201:50887-50906 None:intergenic 35.0%
TGAATCAATGGATGTTGTGA+TGG - tig0012201:50939-50958 None:intergenic 35.0%
GCTTTAGTGCTATGAATCAA+TGG - tig0012201:50951-50970 None:intergenic 35.0%
AAGAAGAAACACCTTGTGAT+GGG + tig0012201:51199-51218 Msa1351330:CDS 35.0%
GTTCAAAAAAAGGAGATGAG+GGG + tig0012201:51304-51323 Msa1351330:CDS 35.0%
! TGCGTTTGAGTTATAGGATT+TGG - tig0012201:51399-51418 None:intergenic 35.0%
! TTACACTGCGTTTGAGTTAT+AGG - tig0012201:51405-51424 None:intergenic 35.0%
TATAACTCAAACGCAGTGTA+AGG + tig0012201:51404-51423 Msa1351330:CDS 35.0%
CATAATCATGATCTAAGTCC+AGG + tig0012201:51489-51508 Msa1351330:CDS 35.0%
TTAGAACTCAATGATCAAGC+TGG + tig0012201:51567-51586 Msa1351330:CDS 35.0%
TAGAACTCAATGATCAAGCT+GGG + tig0012201:51568-51587 Msa1351330:CDS 35.0%
ATGTACTCAAGTTCAAGAAG+AGG + tig0012201:51736-51755 Msa1351330:CDS 35.0%
TGATACCCTTCACAAAAACA+AGG + tig0012201:50741-50760 Msa1351330:CDS 35.0%
! TCAATCAGTCTTTGATTCGT+GGG - tig0012201:50804-50823 None:intergenic 35.0%
! TTCAATCAGTCTTTGATTCG+TGG - tig0012201:50805-50824 None:intergenic 35.0%
ATATGAGATCTGATTCACAG+AGG - tig0012201:50887-50906 None:intergenic 35.0%
TGAATCAATGGATGTTGTGA+TGG - tig0012201:50939-50958 None:intergenic 35.0%
GCTTTAGTGCTATGAATCAA+TGG - tig0012201:50951-50970 None:intergenic 35.0%
AAGAAGAAACACCTTGTGAT+GGG + tig0012201:51199-51218 Msa1351330:CDS 35.0%
GTTCAAAAAAAGGAGATGAG+GGG + tig0012201:51304-51323 Msa1351330:CDS 35.0%
! TGCGTTTGAGTTATAGGATT+TGG - tig0012201:51399-51418 None:intergenic 35.0%
! TTACACTGCGTTTGAGTTAT+AGG - tig0012201:51405-51424 None:intergenic 35.0%
TATAACTCAAACGCAGTGTA+AGG + tig0012201:51404-51423 Msa1351330:CDS 35.0%
CATAATCATGATCTAAGTCC+AGG + tig0012201:51489-51508 Msa1351330:CDS 35.0%
TTAGAACTCAATGATCAAGC+TGG + tig0012201:51567-51586 Msa1351330:CDS 35.0%
TAGAACTCAATGATCAAGCT+GGG + tig0012201:51568-51587 Msa1351330:CDS 35.0%
ATGTACTCAAGTTCAAGAAG+AGG + tig0012201:51736-51755 Msa1351330:CDS 35.0%
!!! CGTAGCCTTGTTTTTGTGAA+GGG - tig0012201:50749-50768 None:intergenic 40.0%
!!! ACGTAGCCTTGTTTTTGTGA+AGG - tig0012201:50750-50769 None:intergenic 40.0%
TGGGAAAGAGGAAACAATTG+CGG - tig0012201:50785-50804 None:intergenic 40.0%
AGCAGAAACAGAGTTATGTG+AGG - tig0012201:50914-50933 None:intergenic 40.0%
GAAGAAGAAACACCTTGTGA+TGG + tig0012201:51198-51217 Msa1351330:CDS 40.0%
GATGTCATTCGGATCTGAAA+AGG + tig0012201:51221-51240 Msa1351330:CDS 40.0%
AAGAATTATGCTGAACGGGT+GGG + tig0012201:51261-51280 Msa1351330:CDS 40.0%
CTAAAATACCTTGCTTTCCC+TGG - tig0012201:51510-51529 None:intergenic 40.0%
! ATATAGGGGCACAAATGAAG+AGG + tig0012201:51541-51560 Msa1351330:CDS 40.0%
TTGCTTCAACGACAAAGGAT+CGG - tig0012201:51614-51633 None:intergenic 40.0%
TCCTTTGTCGTTGAAGCAAA+TGG + tig0012201:51615-51634 Msa1351330:CDS 40.0%
ACCATTTGCTTCAACGACAA+AGG - tig0012201:51619-51638 None:intergenic 40.0%
!!! CGTAGCCTTGTTTTTGTGAA+GGG - tig0012201:50749-50768 None:intergenic 40.0%
!!! ACGTAGCCTTGTTTTTGTGA+AGG - tig0012201:50750-50769 None:intergenic 40.0%
TGGGAAAGAGGAAACAATTG+CGG - tig0012201:50785-50804 None:intergenic 40.0%
AGCAGAAACAGAGTTATGTG+AGG - tig0012201:50914-50933 None:intergenic 40.0%
GAAGAAGAAACACCTTGTGA+TGG + tig0012201:51198-51217 Msa1351330:CDS 40.0%
GATGTCATTCGGATCTGAAA+AGG + tig0012201:51221-51240 Msa1351330:CDS 40.0%
AAGAATTATGCTGAACGGGT+GGG + tig0012201:51261-51280 Msa1351330:CDS 40.0%
CTAAAATACCTTGCTTTCCC+TGG - tig0012201:51510-51529 None:intergenic 40.0%
! ATATAGGGGCACAAATGAAG+AGG + tig0012201:51541-51560 Msa1351330:CDS 40.0%
TTGCTTCAACGACAAAGGAT+CGG - tig0012201:51614-51633 None:intergenic 40.0%
TCCTTTGTCGTTGAAGCAAA+TGG + tig0012201:51615-51634 Msa1351330:CDS 40.0%
ACCATTTGCTTCAACGACAA+AGG - tig0012201:51619-51638 None:intergenic 40.0%
AAAGAGGAAACAATTGCGGC+GGG - tig0012201:50781-50800 None:intergenic 45.0%
GAAAGAGGAAACAATTGCGG+CGG - tig0012201:50782-50801 None:intergenic 45.0%
! GTCTTTGATTCGTGGGAAAG+AGG - tig0012201:50797-50816 None:intergenic 45.0%
! CCTTGTGATGGGATGTCATT+CGG + tig0012201:51210-51229 Msa1351330:CDS 45.0%
CAAGAATTATGCTGAACGGG+TGG + tig0012201:51260-51279 Msa1351330:CDS 45.0%
ATCTAAGTCCAGGGAAAGCA+AGG + tig0012201:51499-51518 Msa1351330:CDS 45.0%
AAAGCAAGACGACTACGACT+TGG + tig0012201:51687-51706 Msa1351330:CDS 45.0%
AAGCAAGACGACTACGACTT+GGG + tig0012201:51688-51707 Msa1351330:CDS 45.0%
AAAGAGGAAACAATTGCGGC+GGG - tig0012201:50781-50800 None:intergenic 45.0%
GAAAGAGGAAACAATTGCGG+CGG - tig0012201:50782-50801 None:intergenic 45.0%
! GTCTTTGATTCGTGGGAAAG+AGG - tig0012201:50797-50816 None:intergenic 45.0%
! CCTTGTGATGGGATGTCATT+CGG + tig0012201:51210-51229 Msa1351330:CDS 45.0%
CAAGAATTATGCTGAACGGG+TGG + tig0012201:51260-51279 Msa1351330:CDS 45.0%
ATCTAAGTCCAGGGAAAGCA+AGG + tig0012201:51499-51518 Msa1351330:CDS 45.0%
AAAGCAAGACGACTACGACT+TGG + tig0012201:51687-51706 Msa1351330:CDS 45.0%
AAGCAAGACGACTACGACTT+GGG + tig0012201:51688-51707 Msa1351330:CDS 45.0%
CCGAATGACATCCCATCACA+AGG - tig0012201:51213-51232 None:intergenic 50.0%
!! TATGCTGAACGGGTGGGTTT+TGG + tig0012201:51267-51286 Msa1351330:CDS 50.0%
! TAGGGGCACAAATGAAGAGG+AGG + tig0012201:51544-51563 Msa1351330:CDS 50.0%
CCGAATGACATCCCATCACA+AGG - tig0012201:51213-51232 None:intergenic 50.0%
!! TATGCTGAACGGGTGGGTTT+TGG + tig0012201:51267-51286 Msa1351330:CDS 50.0%
! TAGGGGCACAAATGAAGAGG+AGG + tig0012201:51544-51563 Msa1351330:CDS 50.0%
! AGACGACTACGACTTGGGAC+AGG + tig0012201:51693-51712 Msa1351330:CDS 55.0%
! AGACGACTACGACTTGGGAC+AGG + tig0012201:51693-51712 Msa1351330:CDS 55.0%
GGAAACAATTGCGGCGGGAG+TGG - tig0012201:50776-50795 None:intergenic 60.0%
GGAAACAATTGCGGCGGGAG+TGG - tig0012201:50776-50795 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
tig0012201 gene 50740 51761 50740 ID=Msa1351330;Name=Msa1351330
tig0012201 mRNA 50740 51761 50740 ID=Msa1351330-mRNA-1;Parent=Msa1351330;Name=Msa1351330-mRNA-1;_AED=0.52;_eAED=0.52;_QI=0|0|0|0.5|0|0.5|2|0|219
tig0012201 exon 50740 50767 50740 ID=Msa1351330-mRNA-1:exon:7678;Parent=Msa1351330-mRNA-1
tig0012201 exon 51130 51761 51130 ID=Msa1351330-mRNA-1:exon:7679;Parent=Msa1351330-mRNA-1
tig0012201 CDS 50740 50767 50740 ID=Msa1351330-mRNA-1:cds;Parent=Msa1351330-mRNA-1
tig0012201 CDS 51130 51761 51130 ID=Msa1351330-mRNA-1:cds;Parent=Msa1351330-mRNA-1
Gene Sequence

>Msa1351330

ATGATACCCTTCACAAAAACAAGGCTACTGCAAGAAGGATTTGAGTCTATTGATGAATTGGAAACTAATGATTGTTTAGAAGCAAATGGTACTTGTGAAGAAGAAACACCTTGTGATGGGATGTCATTCGGATCTGAAAAGGAAATTACCGAGTATTACAAGAATTATGCTGAACGGGTGGGTTTTGGAGTTAAAAAAATAAGTTCAAAAAAAGGAGATGAGGGGAAAATGTATTTTACTTTAGCATGTAATCGAGCTAGAAAGTATGTGAGTCGCTCAAAGAATCTGTTAGAGCCAAATCCTATAACTCAAACGCAGTGTAAGGCTAGATTGAATGCGTGTATTTATTTAGATGGAACAACTAAAATTAAAAGTGTTTTTTTTGAGCATAATCATGATCTAAGTCCAGGGAAAGCAAGGTATTTTAGATCGAACAAGAATATAGGGGCACAAATGAAGAGGAGGTTAGAACTCAATGATCAAGCTGGGATTAATGTAAGTAGAAATTTCCGATCCTTTGTCGTTGAAGCAAATGGTTATGAAAATCTCACATTTGGAGAAAAAGACTGCAGAAACTACATAGACAAAGCAAGACGACTACGACTTGGGACAGGAGATGCTGAAGCAATACAAAATGTACTCAAGTTCAAGAAGAGGTGA

Protein sequence

>Msa1351330

MIPFTKTRLLQEGFESIDELETNDCLEANGTCEEETPCDGMSFGSEKEITEYYKNYAERVGFGVKKISSKKGDEGKMYFTLACNRARKYVSRSKNLLEPNPITQTQCKARLNACIYLDGTTKIKSVFFEHNHDLSPGKARYFRSNKNIGAQMKRRLELNDQAGINVSRNFRSFVVEANGYENLTFGEKDCRNYIDKARRLRLGTGDAEAIQNVLKFKKR*