AlfalfaGEDB | Msa1351400

M. sativa cultivar zhongmu-4 / Msa1351400

KEGG: K07198|K16312|K19852
GO: -
PFAM: PF03101.18

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1351400	XP_024636348.1	82.707	266	15	3	1	235	1	266	9.13e-149	443

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1351400	sp\|Q9SZL8\|FRS5_ARATH	31.474	251	135	5	20	235	76	324	4.22e-28	115

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1351400	A0A072UB45	65.185	270	59	2	1	235	1	270	9.45e-120	353

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TFs/TRs:

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Gene ID	Type	Classification
Msa1351400	TF	FAR1

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1351400	MtrunA17_Chr8g0363841	66.917	266	57	2	1	235	1	266	6.07e-122	361
Msa1351400	MtrunA17_Chr5g0440451	29.323	266	127	7	18	235	44	296	5.15e-30	117
Msa1351400	MtrunA17_Chr1g0202341	31.405	242	130	5	27	235	61	299	8.90e-30	116
Msa1351400	MtrunA17_Chr2g0287651	30.738	244	132	5	27	235	85	326	9.02e-30	116
Msa1351400	MtrunA17_Chr5g0441581	29.555	247	147	5	12	235	27	269	1.13e-28	113
Msa1351400	MtrunA17_Chr2g0296781	49.057	106	54	0	130	235	136	241	2.79e-27	109
Msa1351400	MtrunA17_Chr5g0448761	30.196	255	137	8	20	235	63	315	3.21e-27	109
Msa1351400	MtrunA17_Chr1g0203721	30.315	254	136	7	19	235	65	314	1.44e-26	107
Msa1351400	MtrunA17_Chr6g0458391	28.175	252	157	7	1	235	212	456	2.85e-26	106
Msa1351400	MtrunA17_Chr2g0287061	31.092	238	141	5	7	235	173	396	3.81e-26	106

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1351400	AT4G38180.1	31.474	251	135	5	20	235	76	324	4.29e-29	115
Msa1351400	AT4G19990.3	27.586	261	149	8	1	235	1	247	2.08e-26	107
Msa1351400	AT4G19990.4	27.586	261	149	8	1	235	1	247	2.08e-26	107
Msa1351400	AT4G19990.6	27.586	261	149	8	1	235	1	247	2.25e-26	107
Msa1351400	AT4G19990.2	27.586	261	149	8	1	235	1	247	2.25e-26	107
Msa1351400	AT4G19990.5	27.586	261	149	8	1	235	1	247	2.38e-26	107
Msa1351400	AT4G19990.7	27.626	257	146	8	5	235	19	261	5.35e-26	106
Msa1351400	AT3G06250.2	30.667	225	138	4	16	235	189	400	7.12e-26	105
Msa1351400	AT3G06250.1	30.667	225	138	4	16	235	189	400	7.12e-26	105
Msa1351400	AT3G06250.3	30.667	225	138	4	16	235	189	400	7.12e-26	105

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Find 40 sgRNAs with CRISPR-Local

Find 108 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAGTAGATTATGATTGCTT+TGG	0.344821	tig0012204:-85314	Msa1351400:CDS
AACAAATGTCTTTGCTCAGG+AGG	0.641459	tig0012204:+85650	None:intergenic
AACAGTAAAACTCTTTAAAA+CGG	0.389800	tig0012204:+85830	None:intergenic
AAGGACAAATTGCAAAGCTA+TGG	0.528165	tig0012204:-85746	Msa1351400:CDS
AAGTAGATTATGATTGCTTT+GGG	0.312811	tig0012204:-85313	Msa1351400:CDS
AAGTTGAAATTGATTGAAGC+TGG	0.434164	tig0012204:-85461	Msa1351400:CDS
AATTATTTAGGGGAAGAGGT+CGG	0.592715	tig0012204:-85542	Msa1351400:intron
ACAAATCATGTCATACTTAT+TGG	0.278809	tig0012204:+85259	None:intergenic
ACAGGATTTAGTGTAAGAAA+AGG	0.440176	tig0012204:-85889	Msa1351400:CDS
ACAGTAAAACTCTTTAAAAC+GGG	0.452807	tig0012204:+85831	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
TGTCAATCAGTTGAAATTGA+TGG	-	tig0012204:85244-85263	Msa1351400:CDS	30.0%
ACAGGATTTAGTGTAAGAAA+AGG	-	tig0012204:85346-85365	Msa1351400:CDS	30.0%
CAGGATTTAGTGTAAGAAAA+GGG	-	tig0012204:85347-85366	Msa1351400:CDS	30.0%
ATTTAGTGTAAGAAAAGGGA+GGG	-	tig0012204:85351-85370	Msa1351400:CDS	30.0%
GACATTTGACATATTTCTCA+TGG	+	tig0012204:85616-85635	None:intergenic	30.0%
AATTCACCATAGACTTTATG+AGG	+	tig0012204:85647-85666	None:intergenic	30.0%
ATAAAGTCTATGGTGAATTC+TGG	-	tig0012204:85648-85667	Msa1351400:CDS	30.0%
AAGTTGAAATTGATTGAAGC+TGG	-	tig0012204:85774-85793	Msa1351400:CDS	30.0%
AGTTGAAATTGATTGAAGCT+GGG	-	tig0012204:85775-85794	Msa1351400:CDS	30.0%
AGTAGATTATGATTGCTTTG+GGG	-	tig0012204:85923-85942	Msa1351400:CDS	30.0%

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Chromosome	Type	Strat	End	Strand	Name
tig0012204	gene	85229	86028	85229	ID=Msa1351400;Name=Msa1351400
tig0012204	mRNA	85229	86028	85229	ID=Msa1351400-mRNA-1;Parent=Msa1351400;Name=Msa1351400-mRNA-1;_AED=0.09;_eAED=0.14;_QI=0\|0\|0\|1\|1\|1\|2\|0\|235
tig0012204	exon	85647	86028	85647	ID=Msa1351400-mRNA-1:exon:7730;Parent=Msa1351400-mRNA-1
tig0012204	exon	85229	85554	85229	ID=Msa1351400-mRNA-1:exon:7729;Parent=Msa1351400-mRNA-1
tig0012204	CDS	85647	86028	85647	ID=Msa1351400-mRNA-1:cds;Parent=Msa1351400-mRNA-1
tig0012204	CDS	85229	85554	85229	ID=Msa1351400-mRNA-1:cds;Parent=Msa1351400-mRNA-1

Gene Sequence

>Msa1351400

ATGGAGATAAATGAATGTCAATCAGTTGAAATTGATGGATGCAAAGATATTTTTCTTGGTCAAGTTGTGAATAACAAAGATGAAGCTTATAACTTATATCAAGCACATGCCTTCAAAACAGGATTTAGTGTAAGAAAAGGGAGGGAGTTATATTATGATAATGAGAGAAAAATCACCCGTTTTAAAGAGTTTTACTGTTCCAAAGAAGGGTTTAAGAATAATGAATATGGAGGTGAAGTGGCTTATGAAAGAGCTGATTCAAGGACAAATTGCAAAGCTATGGTGAGGTTTAATGTTAGCAAGGAAGGAGTTTGGAAAGTTACAAAGTTTCTATTAGATCAAATCATCAACTTGTACCTCCTGAGCAAAGACATTTGTTGCGGGGAAGAGGTCGGTGGATATAACAATCTTGGTATAACATTGAAAGATATGCATAATTATGTCTACACTGAAAAGTTGAAATTGATTGAAGCTGGGGATGCTCAATCTTTAGTAAATAACTTACAAAATAGACAAGCACAAGATCCTATGTTCTATTACAGTGTGCAGCTAGATCAGGAATCTCGTTTGACTAATGTCTTTTGGAGAGATGGTAAATCTAAAGTAGATTATGATTGCTTTGGGGATGTTGTGATATTTGACACTACTTATCGTACCAATAAGTATGACATGATTTGTGCCCCATTTGTTGGGATAAATCATCATTGA

Protein sequence

>Msa1351400

MEINECQSVEIDGCKDIFLGQVVNNKDEAYNLYQAHAFKTGFSVRKGRELYYDNERKITRFKEFYCSKEGFKNNEYGGEVAYERADSRTNCKAMVRFNVSKEGVWKVTKFLLDQIINLYLLSKDICCGEEVGGYNNLGITLKDMHNYVYTEKLKLIEAGDAQSLVNNLQNRQAQDPMFYYSVQLDQESRLTNVFWRDGKSKVDYDCFGDVVIFDTTYRTNKYDMICAPFVGINHH*