Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1352650 | KEH20878.1 | 93.750 | 128 | 7 | 1 | 1 | 127 | 1 | 128 | 3.32e-81 | 244 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1352650 | sp|Q93WU9|WRK51_ARATH | 68.421 | 38 | 12 | 0 | 90 | 127 | 93 | 130 | 3.20e-12 | 63.5 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1352650 | A0A072TVK6 | 93.750 | 128 | 7 | 1 | 1 | 127 | 1 | 128 | 1.59e-81 | 244 |
| Gene ID | Type | Classification |
|---|---|---|
| Msa1352650 | TF | WRKY |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1352650 | MtrunA17_Chr8g0383251 | 93.750 | 128 | 7 | 1 | 1 | 127 | 1 | 128 | 2.13e-84 | 244 |
| Msa1352650 | MtrunA17_Chr3g0129321 | 47.518 | 141 | 57 | 6 | 1 | 127 | 1 | 138 | 4.33e-25 | 94.4 |
| Msa1352650 | MtrunA17_Chr1g0150801 | 39.000 | 100 | 59 | 1 | 28 | 127 | 25 | 122 | 1.33e-14 | 66.2 |
| Msa1352650 | MtrunA17_Chr4g0004911 | 32.231 | 121 | 74 | 3 | 8 | 127 | 243 | 356 | 2.13e-11 | 59.7 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1352650 | AT5G64810.1 | 68.421 | 38 | 12 | 0 | 90 | 127 | 93 | 130 | 3.26e-13 | 63.5 |
| Msa1352650 | AT5G26170.1 | 70.588 | 34 | 10 | 0 | 94 | 127 | 100 | 133 | 1.13e-12 | 61.6 |
Find 28 sgRNAs with CRISPR-Local
Find 114 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGCATAGATAAACTCAGTTT+TGG | 0.232272 | tig0012674:-18153 | None:intergenic |
| AAGATCAGAGCTTGAGATTA+TGG | 0.284446 | tig0012674:+18865 | Msa1352650:CDS |
| TCCTAACCTAAGGTAATAAA+AGG | 0.295844 | tig0012674:+18937 | Msa1352650:exon |
| CTTGTTCTAAAGGCTATCTT+TGG | 0.312484 | tig0012674:-18845 | None:intergenic |
| GTAAACTCAGAAGAGGAATT+AGG | 0.336749 | tig0012674:-18239 | None:intergenic |
| TGAGTAGAGTGAGCAAAGTT+TGG | 0.356689 | tig0012674:-18209 | None:intergenic |
| TGCATCATTGAATTCTTGAT+TGG | 0.365316 | tig0012674:-18366 | None:intergenic |
| GAGTAGAGTGAGCAAAGTTT+GGG | 0.391273 | tig0012674:-18208 | None:intergenic |
| TTACATATTATTGTTCTTTG+AGG | 0.400718 | tig0012674:-18390 | None:intergenic |
| TGTTACCTTCTCTAATGATT+CGG | 0.455426 | tig0012674:-18333 | None:intergenic |
| AAGCTCTGATCTTGTTCTAA+AGG | 0.476991 | tig0012674:-18855 | None:intergenic |
| GGATACAAGTGGAGGAAGTA+CGG | 0.476991 | tig0012674:+18893 | Msa1352650:CDS |
| GTGACCGAGATTCTTGATCA+AGG | 0.501478 | tig0012674:-18304 | None:intergenic |
| AAGCGAATTAAAGTAGAAGC+GGG | 0.522259 | tig0012674:+18821 | Msa1352650:CDS |
| AACTGAGTTTATCTATGCAA+TGG | 0.530683 | tig0012674:+18157 | Msa1352650:exon |
| CAAAGTTTGGGGTAGCATGA+GGG | 0.552088 | tig0012674:-18196 | None:intergenic |
| GATACAAGTGGAGGAAGTAC+GGG | 0.554483 | tig0012674:+18894 | Msa1352650:CDS |
| ATCACCTTGATCAAGAATCT+CGG | 0.560454 | tig0012674:+18300 | Msa1352650:CDS |
| TGAAGAACAGTCCTAACCTA+AGG | 0.563814 | tig0012674:+18927 | Msa1352650:CDS |
| GCAAAGTTTGGGGTAGCATG+AGG | 0.576767 | tig0012674:-18197 | None:intergenic |
| AGATAGTGTAAACTCAGAAG+AGG | 0.584693 | tig0012674:-18246 | None:intergenic |
| AAGTACCGAATCATTAGAGA+AGG | 0.606756 | tig0012674:+18328 | Msa1352650:CDS |
| AGTAGAGTGAGCAAAGTTTG+GGG | 0.609244 | tig0012674:-18207 | None:intergenic |
| GAGCTTGAGATTATGGACGA+TGG | 0.615286 | tig0012674:+18872 | Msa1352650:CDS |
| TTATGGACGATGGATACAAG+TGG | 0.616058 | tig0012674:+18882 | Msa1352650:CDS |
| TAAGCGAATTAAAGTAGAAG+CGG | 0.631449 | tig0012674:+18820 | Msa1352650:CDS |
| TGGACGATGGATACAAGTGG+AGG | 0.691711 | tig0012674:+18885 | Msa1352650:CDS |
| AGCGAATTAAAGTAGAAGCG+GGG | 0.700406 | tig0012674:+18822 | Msa1352650:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TTCATTTAAAATTAAGAAAA+GGG | + | tig0012674:18444-18463 | Msa1352650:intron | 10.0% |
| !!! | TTCATTTAAAATTAAGAAAA+GGG | + | tig0012674:18444-18463 | Msa1352650:intron | 10.0% |
| !!! | GTTCATTTAAAATTAAGAAA+AGG | + | tig0012674:18443-18462 | Msa1352650:intron | 15.0% |
| !!! | GTTCATTTAAAATTAAGAAA+AGG | + | tig0012674:18443-18462 | Msa1352650:intron | 15.0% |
| !! | TTACATATTATTGTTCTTTG+AGG | - | tig0012674:18393-18412 | None:intergenic | 20.0% |
| !!! | CTAATAAAGTGTACATATTT+TGG | + | tig0012674:18754-18773 | Msa1352650:intron | 20.0% |
| !!! | TTTTGCAGAAAATACAAAAA+TGG | + | tig0012674:18794-18813 | Msa1352650:intron | 20.0% |
| !! | TTTGCAGAAAATACAAAAAT+GGG | + | tig0012674:18795-18814 | Msa1352650:intron | 20.0% |
| !! | TTACATATTATTGTTCTTTG+AGG | - | tig0012674:18393-18412 | None:intergenic | 20.0% |
| !!! | CTAATAAAGTGTACATATTT+TGG | + | tig0012674:18754-18773 | Msa1352650:intron | 20.0% |
| !!! | TTTTGCAGAAAATACAAAAA+TGG | + | tig0012674:18794-18813 | Msa1352650:intron | 20.0% |
| !! | TTTGCAGAAAATACAAAAAT+GGG | + | tig0012674:18795-18814 | Msa1352650:intron | 20.0% |
| ! | ACAAGAGCAATACAAATTAA+GGG | - | tig0012674:18425-18444 | None:intergenic | 25.0% |
| ! | AACAAGAGCAATACAAATTA+AGG | - | tig0012674:18426-18445 | None:intergenic | 25.0% |
| !! | TTCAAAAGTGTACTTCTAAA+CGG | - | tig0012674:18507-18526 | None:intergenic | 25.0% |
| !!! | TCTAAAAGTGTACATTTTGA+GGG | + | tig0012674:18576-18595 | Msa1352650:intron | 25.0% |
| ! | ATTAATGAAGCATATACCAT+CGG | + | tig0012674:18688-18707 | Msa1352650:intron | 25.0% |
| !! | GTATTTCCTTTTATTACCTT+AGG | - | tig0012674:18946-18965 | None:intergenic | 25.0% |
| ! | ACAAGAGCAATACAAATTAA+GGG | - | tig0012674:18425-18444 | None:intergenic | 25.0% |
| ! | AACAAGAGCAATACAAATTA+AGG | - | tig0012674:18426-18445 | None:intergenic | 25.0% |
| !! | TTCAAAAGTGTACTTCTAAA+CGG | - | tig0012674:18507-18526 | None:intergenic | 25.0% |
| !!! | TCTAAAAGTGTACATTTTGA+GGG | + | tig0012674:18576-18595 | Msa1352650:intron | 25.0% |
| ! | ATTAATGAAGCATATACCAT+CGG | + | tig0012674:18688-18707 | Msa1352650:intron | 25.0% |
| !! | GTATTTCCTTTTATTACCTT+AGG | - | tig0012674:18946-18965 | None:intergenic | 25.0% |
| !!! | TTTTGGGTTGTTGGTATAAA+TGG | - | tig0012674:18139-18158 | None:intergenic | 30.0% |
| ! | GCATAGATAAACTCAGTTTT+GGG | - | tig0012674:18155-18174 | None:intergenic | 30.0% |
| ! | TGCATAGATAAACTCAGTTT+TGG | - | tig0012674:18156-18175 | None:intergenic | 30.0% |
| AACTGAGTTTATCTATGCAA+TGG | + | tig0012674:18157-18176 | Msa1352650:exon | 30.0% | |
| TGTTACCTTCTCTAATGATT+CGG | - | tig0012674:18336-18355 | None:intergenic | 30.0% | |
| TGCATCATTGAATTCTTGAT+TGG | - | tig0012674:18369-18388 | None:intergenic | 30.0% | |
| !! | ATCGGTTTTTAAGAAACTAC+GGG | - | tig0012674:18483-18502 | None:intergenic | 30.0% |
| !! | GATCGGTTTTTAAGAAACTA+CGG | - | tig0012674:18484-18503 | None:intergenic | 30.0% |
| ! | TCAAAAGTGTACTTCTAAAC+GGG | - | tig0012674:18506-18525 | None:intergenic | 30.0% |
| !! | GTCTAAAAGTGTACATTTTG+AGG | + | tig0012674:18575-18594 | Msa1352650:intron | 30.0% |
| !!! | TGCATGATTGCATTTTTTCA+AGG | - | tig0012674:18637-18656 | None:intergenic | 30.0% |
| !! | AGTGTACATATTTTGGATTG+TGG | + | tig0012674:18761-18780 | Msa1352650:intron | 30.0% |
| !! | GTGTACATATTTTGGATTGT+GGG | + | tig0012674:18762-18781 | Msa1352650:intron | 30.0% |
| !! | TGTACATATTTTGGATTGTG+GGG | + | tig0012674:18763-18782 | Msa1352650:intron | 30.0% |
| TAAGCGAATTAAAGTAGAAG+CGG | + | tig0012674:18820-18839 | Msa1352650:CDS | 30.0% | |
| TCCTAACCTAAGGTAATAAA+AGG | + | tig0012674:18937-18956 | Msa1352650:exon | 30.0% | |
| ! | TCCTTTTATTACCTTAGGTT+AGG | - | tig0012674:18941-18960 | None:intergenic | 30.0% |
| !!! | TTTTGGGTTGTTGGTATAAA+TGG | - | tig0012674:18139-18158 | None:intergenic | 30.0% |
| ! | GCATAGATAAACTCAGTTTT+GGG | - | tig0012674:18155-18174 | None:intergenic | 30.0% |
| ! | TGCATAGATAAACTCAGTTT+TGG | - | tig0012674:18156-18175 | None:intergenic | 30.0% |
| AACTGAGTTTATCTATGCAA+TGG | + | tig0012674:18157-18176 | Msa1352650:exon | 30.0% | |
| TGTTACCTTCTCTAATGATT+CGG | - | tig0012674:18336-18355 | None:intergenic | 30.0% | |
| TGCATCATTGAATTCTTGAT+TGG | - | tig0012674:18369-18388 | None:intergenic | 30.0% | |
| !! | ATCGGTTTTTAAGAAACTAC+GGG | - | tig0012674:18483-18502 | None:intergenic | 30.0% |
| !! | GATCGGTTTTTAAGAAACTA+CGG | - | tig0012674:18484-18503 | None:intergenic | 30.0% |
| ! | TCAAAAGTGTACTTCTAAAC+GGG | - | tig0012674:18506-18525 | None:intergenic | 30.0% |
| !! | GTCTAAAAGTGTACATTTTG+AGG | + | tig0012674:18575-18594 | Msa1352650:intron | 30.0% |
| !!! | TGCATGATTGCATTTTTTCA+AGG | - | tig0012674:18637-18656 | None:intergenic | 30.0% |
| !! | AGTGTACATATTTTGGATTG+TGG | + | tig0012674:18761-18780 | Msa1352650:intron | 30.0% |
| !! | GTGTACATATTTTGGATTGT+GGG | + | tig0012674:18762-18781 | Msa1352650:intron | 30.0% |
| !! | TGTACATATTTTGGATTGTG+GGG | + | tig0012674:18763-18782 | Msa1352650:intron | 30.0% |
| TAAGCGAATTAAAGTAGAAG+CGG | + | tig0012674:18820-18839 | Msa1352650:CDS | 30.0% | |
| TCCTAACCTAAGGTAATAAA+AGG | + | tig0012674:18937-18956 | Msa1352650:exon | 30.0% | |
| ! | TCCTTTTATTACCTTAGGTT+AGG | - | tig0012674:18941-18960 | None:intergenic | 30.0% |
| !!! | TAAACTCAGTTTTGGGTTGT+TGG | - | tig0012674:18148-18167 | None:intergenic | 35.0% |
| GTAAACTCAGAAGAGGAATT+AGG | - | tig0012674:18242-18261 | None:intergenic | 35.0% | |
| AGATAGTGTAAACTCAGAAG+AGG | - | tig0012674:18249-18268 | None:intergenic | 35.0% | |
| ATCACCTTGATCAAGAATCT+CGG | + | tig0012674:18300-18319 | Msa1352650:CDS | 35.0% | |
| !! | AAGTACCGAATCATTAGAGA+AGG | + | tig0012674:18328-18347 | Msa1352650:CDS | 35.0% |
| GCAATACAAATTAAGGGAGA+AGG | - | tig0012674:18419-18438 | None:intergenic | 35.0% | |
| !! | TCGGTTTTTAAGAAACTACG+GGG | - | tig0012674:18482-18501 | None:intergenic | 35.0% |
| !! | AAGTGTACATTTTGAGGGAT+CGG | + | tig0012674:18581-18600 | Msa1352650:intron | 35.0% |
| !! | GGATCGGTTTTTAGAAATCA+TGG | + | tig0012674:18597-18616 | Msa1352650:intron | 35.0% |
| CAAATGAAGTAATGAACCGA+TGG | - | tig0012674:18707-18726 | None:intergenic | 35.0% | |
| AAGCGAATTAAAGTAGAAGC+GGG | + | tig0012674:18821-18840 | Msa1352650:CDS | 35.0% | |
| !! | CTTGTTCTAAAGGCTATCTT+TGG | - | tig0012674:18848-18867 | None:intergenic | 35.0% |
| ! | AAGCTCTGATCTTGTTCTAA+AGG | - | tig0012674:18858-18877 | None:intergenic | 35.0% |
| AAGATCAGAGCTTGAGATTA+TGG | + | tig0012674:18865-18884 | Msa1352650:CDS | 35.0% | |
| !!! | TAAACTCAGTTTTGGGTTGT+TGG | - | tig0012674:18148-18167 | None:intergenic | 35.0% |
| GTAAACTCAGAAGAGGAATT+AGG | - | tig0012674:18242-18261 | None:intergenic | 35.0% | |
| AGATAGTGTAAACTCAGAAG+AGG | - | tig0012674:18249-18268 | None:intergenic | 35.0% | |
| ATCACCTTGATCAAGAATCT+CGG | + | tig0012674:18300-18319 | Msa1352650:CDS | 35.0% | |
| !! | AAGTACCGAATCATTAGAGA+AGG | + | tig0012674:18328-18347 | Msa1352650:CDS | 35.0% |
| GCAATACAAATTAAGGGAGA+AGG | - | tig0012674:18419-18438 | None:intergenic | 35.0% | |
| !! | TCGGTTTTTAAGAAACTACG+GGG | - | tig0012674:18482-18501 | None:intergenic | 35.0% |
| !! | AAGTGTACATTTTGAGGGAT+CGG | + | tig0012674:18581-18600 | Msa1352650:intron | 35.0% |
| !! | GGATCGGTTTTTAGAAATCA+TGG | + | tig0012674:18597-18616 | Msa1352650:intron | 35.0% |
| CAAATGAAGTAATGAACCGA+TGG | - | tig0012674:18707-18726 | None:intergenic | 35.0% | |
| AAGCGAATTAAAGTAGAAGC+GGG | + | tig0012674:18821-18840 | Msa1352650:CDS | 35.0% | |
| !! | CTTGTTCTAAAGGCTATCTT+TGG | - | tig0012674:18848-18867 | None:intergenic | 35.0% |
| ! | AAGCTCTGATCTTGTTCTAA+AGG | - | tig0012674:18858-18877 | None:intergenic | 35.0% |
| AAGATCAGAGCTTGAGATTA+TGG | + | tig0012674:18865-18884 | Msa1352650:CDS | 35.0% | |
| AGTAGAGTGAGCAAAGTTTG+GGG | - | tig0012674:18210-18229 | None:intergenic | 40.0% | |
| GAGTAGAGTGAGCAAAGTTT+GGG | - | tig0012674:18211-18230 | None:intergenic | 40.0% | |
| TGAGTAGAGTGAGCAAAGTT+TGG | - | tig0012674:18212-18231 | None:intergenic | 40.0% | |
| AGTGTACTTCTAAACGGGAT+CGG | - | tig0012674:18501-18520 | None:intergenic | 40.0% | |
| AGCGAATTAAAGTAGAAGCG+GGG | + | tig0012674:18822-18841 | Msa1352650:CDS | 40.0% | |
| TTATGGACGATGGATACAAG+TGG | + | tig0012674:18882-18901 | Msa1352650:CDS | 40.0% | |
| TGAAGAACAGTCCTAACCTA+AGG | + | tig0012674:18927-18946 | Msa1352650:CDS | 40.0% | |
| AGTAGAGTGAGCAAAGTTTG+GGG | - | tig0012674:18210-18229 | None:intergenic | 40.0% | |
| GAGTAGAGTGAGCAAAGTTT+GGG | - | tig0012674:18211-18230 | None:intergenic | 40.0% | |
| TGAGTAGAGTGAGCAAAGTT+TGG | - | tig0012674:18212-18231 | None:intergenic | 40.0% | |
| AGTGTACTTCTAAACGGGAT+CGG | - | tig0012674:18501-18520 | None:intergenic | 40.0% | |
| AGCGAATTAAAGTAGAAGCG+GGG | + | tig0012674:18822-18841 | Msa1352650:CDS | 40.0% | |
| TTATGGACGATGGATACAAG+TGG | + | tig0012674:18882-18901 | Msa1352650:CDS | 40.0% | |
| TGAAGAACAGTCCTAACCTA+AGG | + | tig0012674:18927-18946 | Msa1352650:CDS | 40.0% | |
| ! | CAAAGTTTGGGGTAGCATGA+GGG | - | tig0012674:18199-18218 | None:intergenic | 45.0% |
| GTGACCGAGATTCTTGATCA+AGG | - | tig0012674:18307-18326 | None:intergenic | 45.0% | |
| GAGCTTGAGATTATGGACGA+TGG | + | tig0012674:18872-18891 | Msa1352650:CDS | 45.0% | |
| GGATACAAGTGGAGGAAGTA+CGG | + | tig0012674:18893-18912 | Msa1352650:CDS | 45.0% | |
| ! | GATACAAGTGGAGGAAGTAC+GGG | + | tig0012674:18894-18913 | Msa1352650:CDS | 45.0% |
| ! | CAAAGTTTGGGGTAGCATGA+GGG | - | tig0012674:18199-18218 | None:intergenic | 45.0% |
| GTGACCGAGATTCTTGATCA+AGG | - | tig0012674:18307-18326 | None:intergenic | 45.0% | |
| GAGCTTGAGATTATGGACGA+TGG | + | tig0012674:18872-18891 | Msa1352650:CDS | 45.0% | |
| GGATACAAGTGGAGGAAGTA+CGG | + | tig0012674:18893-18912 | Msa1352650:CDS | 45.0% | |
| ! | GATACAAGTGGAGGAAGTAC+GGG | + | tig0012674:18894-18913 | Msa1352650:CDS | 45.0% |
| ! | GCAAAGTTTGGGGTAGCATG+AGG | - | tig0012674:18200-18219 | None:intergenic | 50.0% |
| TGGACGATGGATACAAGTGG+AGG | + | tig0012674:18885-18904 | Msa1352650:CDS | 50.0% | |
| ! | GCAAAGTTTGGGGTAGCATG+AGG | - | tig0012674:18200-18219 | None:intergenic | 50.0% |
| TGGACGATGGATACAAGTGG+AGG | + | tig0012674:18885-18904 | Msa1352650:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| tig0012674 | gene | 18109 | 18984 | 18109 | ID=Msa1352650;Name=Msa1352650 |
| tig0012674 | mRNA | 18109 | 18984 | 18109 | ID=Msa1352650-mRNA-1;Parent=Msa1352650;Name=Msa1352650-mRNA-1;_AED=0.02;_eAED=0.02;_QI=67|1|1|1|1|1|2|32|127 |
| tig0012674 | exon | 18109 | 18408 | 18109 | ID=Msa1352650-mRNA-1:exon:8271;Parent=Msa1352650-mRNA-1 |
| tig0012674 | exon | 18802 | 18984 | 18802 | ID=Msa1352650-mRNA-1:exon:8272;Parent=Msa1352650-mRNA-1 |
| tig0012674 | five_prime_UTR | 18109 | 18175 | 18109 | ID=Msa1352650-mRNA-1:five_prime_utr;Parent=Msa1352650-mRNA-1 |
| tig0012674 | CDS | 18176 | 18408 | 18176 | ID=Msa1352650-mRNA-1:cds;Parent=Msa1352650-mRNA-1 |
| tig0012674 | CDS | 18802 | 18952 | 18802 | ID=Msa1352650-mRNA-1:cds;Parent=Msa1352650-mRNA-1 |
| tig0012674 | three_prime_UTR | 18953 | 18984 | 18953 | ID=Msa1352650-mRNA-1:three_prime_utr;Parent=Msa1352650-mRNA-1 |
| Gene Sequence |
| Protein sequence |
