AlfalfaGEDB | Msa1352680

M. sativa cultivar zhongmu-4 / Msa1352680

KEGG: K17604
GO: -
PFAM: PF03101.18

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1352680	GAU40839.1	80.056	356	67	3	16	370	30	382	0.0	606

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1352680	sp\|Q9SWG3\|FAR1_ARATH	33.815	346	223	4	31	371	33	377	2.39e-67	230

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1352680	A0A2Z6P9Q3	80.056	356	67	3	16	370	30	382	0.0	606

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TFs/TRs:

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Gene ID	Type	Classification
Msa1352680	TF	FAR1

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
No data available in table

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1352680	MtrunA17_Chr2g0296781	78.165	316	68	1	53	368	1	315	0.0	539
Msa1352680	MtrunA17_Chr1g0196541	63.438	320	116	1	53	372	1	319	1.09e-150	442
Msa1352680	MtrunA17_Chr6g0477261	84.951	206	31	0	165	370	1	206	1.36e-130	389
Msa1352680	MtrunA17_Chr5g0441581	49.701	334	154	4	44	372	23	347	1.67e-108	333
Msa1352680	MtrunA17_Chr1g0202341	38.438	333	190	5	48	371	50	376	2.36e-75	246
Msa1352680	MtrunA17_Chr2g0291591	35.817	349	219	3	18	364	33	378	9.27e-75	241
Msa1352680	MtrunA17_Chr2g0329031	37.685	337	194	6	41	372	6	331	5.73e-74	243
Msa1352680	MtrunA17_Chr5g0448761	37.135	342	203	7	43	373	54	394	6.84e-71	236
Msa1352680	MtrunA17_Chr2g0291581	37.249	349	202	6	27	371	47	382	2.53e-69	230
Msa1352680	MtrunA17_Chr1g0207551	34.356	326	211	2	46	369	31	355	4.46e-66	223

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1352680	AT4G15090.1	33.815	346	223	4	31	371	33	377	2.43e-68	230
Msa1352680	AT4G15090.5	33.815	346	223	4	31	371	33	377	2.43e-68	230
Msa1352680	AT4G15090.3	33.815	346	223	4	31	371	33	377	2.43e-68	230
Msa1352680	AT4G15090.2	33.815	346	223	4	31	371	33	377	2.43e-68	230
Msa1352680	AT4G15090.4	33.815	346	223	4	31	371	33	377	2.43e-68	230
Msa1352680	AT4G38180.1	33.623	345	221	4	36	373	60	403	1.10e-65	223
Msa1352680	AT1G76320.3	36.875	320	189	5	53	368	1	311	1.66e-65	220
Msa1352680	AT1G76320.1	36.875	320	189	5	53	368	1	311	3.61e-65	220
Msa1352680	AT1G76320.4	36.875	320	189	5	53	368	1	311	3.61e-65	220
Msa1352680	AT1G76320.2	36.875	320	189	5	53	368	1	311	4.00e-65	220

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Find 55 sgRNAs with CRISPR-Local

Find 190 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAGTGAGGCGGCTTCGACT+TGG	0.518819	tig0012682:+13570	Msa1352680:CDS
AAATGAAGATGATGAACCTA+AGG	0.544931	tig0012682:+13074	Msa1352680:CDS
AAGAATTATGCAAGATGTTT+AGG	0.244212	tig0012682:+13141	Msa1352680:CDS
AAGTGAGGCGGCTTCGACTT+GGG	0.432744	tig0012682:+13571	Msa1352680:CDS
AATGTATGTGTGTCTTCAGA+TGG	0.571681	tig0012682:+13315	Msa1352680:CDS
ACAAGCCATAATTACTGTCC+AGG	0.535094	tig0012682:+13932	Msa1352680:CDS
ACCACCGATGGCGAGCTTTC+GGG	0.377799	tig0012682:-13989	None:intergenic
ACTACATTGACAAAGTGAGG+CGG	0.739143	tig0012682:+13559	Msa1352680:CDS
ACTCCAACAAAAGGAGCAAA+AGG	0.476605	tig0012682:-13795	None:intergenic
ACTTGGTTAGAATGTATGCG+TGG	0.623148	tig0012682:+13900	Msa1352680:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AACATATAAGGTTATGGAGA+AGG	-	tig0012682:12694-12713	None:intergenic	30.0%
TTGAACTGGATGCTAAAATA+GGG	-	tig0012682:12853-12872	None:intergenic	30.0%
GAATTAAAAGTAAAGGCAAC+AGG	-	tig0012682:12927-12946	None:intergenic	30.0%
AAATGAAGATGATGAACCTA+AGG	+	tig0012682:13074-13093	Msa1352680:CDS	30.0%
TTAAATTTCATGCCAACCTT+AGG	-	tig0012682:13093-13112	None:intergenic	30.0%
TAGTATAAAAAGGAGATTGG+AGG	+	tig0012682:13434-13453	Msa1352680:CDS	30.0%
GGATATGATAACCTTACATT+CGG	+	tig0012682:13519-13538	Msa1352680:CDS	30.0%
GATATGATAACCTTACATTC+GGG	+	tig0012682:13520-13539	Msa1352680:CDS	30.0%
ATATGATAACCTTACATTCG+GGG	+	tig0012682:13521-13540	Msa1352680:CDS	30.0%
TGATGATTTACTCCAACAAA+AGG	-	tig0012682:13807-13826	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
tig0012682	gene	12635	14067	12635	ID=Msa1352680;Name=Msa1352680
tig0012682	mRNA	12635	14067	12635	ID=Msa1352680-mRNA-1;Parent=Msa1352680;Name=Msa1352680-mRNA-1;_AED=0.11;_eAED=0.29;_QI=0\|0\|0\|0.5\|1\|1\|2\|0\|373
tig0012682	exon	12635	12680	12635	ID=Msa1352680-mRNA-1:exon:8280;Parent=Msa1352680-mRNA-1
tig0012682	exon	12992	14067	12992	ID=Msa1352680-mRNA-1:exon:8281;Parent=Msa1352680-mRNA-1
tig0012682	CDS	12635	12680	12635	ID=Msa1352680-mRNA-1:cds;Parent=Msa1352680-mRNA-1
tig0012682	CDS	12992	14067	12992	ID=Msa1352680-mRNA-1:cds;Parent=Msa1352680-mRNA-1

Gene Sequence

>Msa1352680

ATGGAAGAGACAATCACGGCACACGTTTTGATTTTGGACAATCACGTGGAGAAAACTTTTAGATCTTTTGAAGAAGATGAAGAAGAGAGTGATCACATAAATGAAAATTGTGAGGATAATTTAACAGAAAATGAAGATGATGAACCTAAGGTTGGCATGAAATTTAATTCTGCAGACGAAGTTGACAAATATTACAAGAATTATGCAAGATGTTTAGGTTTTGGAGTTAGCAAAATCAGTACAAAAAAGGGAGATGATGGAAAAAAGTATTTTACTCTAGGGTGTAACCGTGCAAGAAGCTATGTGAGTAAGTCCAAAAATCTTTTAAAGCCAAATCCTAGCATGAAAACACACTGTAAGGCTAGAATAAATGTATGTGTGTCTTCAGATGGAACAATTACTATTTCTAAGGTTGCTCTTGAACATAATCATGACTTAATAAGTCCAAGCAAATCAAGATATTTTAGATGCAACAAGAATATAGACCCTAGTATAAAAAGGAGATTGGAGGTTAATGACCAAGCTGGGATTAATGTAAGTAGAAATTTTCGGTCTATGGTTGTTGAAGCAAGTGGATATGATAACCTTACATTCGGGGAAAAAGATGTTAGAAACTACATTGACAAAGTGAGGCGGCTTCGACTTGGGACAGGAGACGCTGAAGCCATTCAAAATTATTTTGTTAGAATGCAAAGACAAAATAGTGAATTTTATTATGTAATGGATGTGGATGACAAAAGTCGTTTACGAAATGTTTTTTGGGCAGATGCGAGATGTAGGGCTGCTTATGAGTATTTTGGTGAAGTAATAACCTTTGACACCACTTACTTAACAAATAAATATGACATGCCTTTTGCTCCTTTTGTTGGAGTAAATCATCATGGTCAGTCAGTTCTGTTAGGTTGCGCCCTATTGTCGAAGGAGGATACTAAAACTTTTTCTTGGTTGTTCACTACTTGGTTAGAATGTATGCGTGGGCGTGCTCCACAAGCCATAATTACTGTCCAGGATGGAGCAATGAAGAATGCAGTTGAGGCCGTCTTCCCGAAAGCTCGCCATCGGTGGTGCTTGTGGCATATAATGAAAAAGGTTCCAGAAAAATTGAGTAGACTCGCAATATAG

Protein sequence

>Msa1352680

MEETITAHVLILDNHVEKTFRSFEEDEEESDHINENCEDNLTENEDDEPKVGMKFNSADEVDKYYKNYARCLGFGVSKISTKKGDDGKKYFTLGCNRARSYVSKSKNLLKPNPSMKTHCKARINVCVSSDGTITISKVALEHNHDLISPSKSRYFRCNKNIDPSIKRRLEVNDQAGINVSRNFRSMVVEASGYDNLTFGEKDVRNYIDKVRRLRLGTGDAEAIQNYFVRMQRQNSEFYYVMDVDDKSRLRNVFWADARCRAAYEYFGEVITFDTTYLTNKYDMPFAPFVGVNHHGQSVLLGCALLSKEDTKTFSWLFTTWLECMRGRAPQAIITVQDGAMKNAVEAVFPKARHRWCLWHIMKKVPEKLSRLAI*