Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1353320 | PNX89196.1 | 74.866 | 187 | 47 | 0 | 22 | 208 | 7 | 193 | 8.89e-95 | 287 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1353320 | sp|Q9LKR4|FRS10_ARATH | 33.951 | 162 | 103 | 3 | 50 | 208 | 61 | 221 | 1.08e-18 | 87.0 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1353320 | A0A2K3MEL2 | 74.866 | 187 | 47 | 0 | 22 | 208 | 7 | 193 | 4.25e-95 | 287 |
| Gene ID | Type | Classification |
|---|---|---|
| Msa1353320 | TF | FAR1 |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1353320 | MtrunA17_Chr1g0203051 | 37.864 | 206 | 126 | 1 | 2 | 207 | 39 | 242 | 2.66e-49 | 169 |
| Msa1353320 | MtrunA17_Chr1g0171741 | 40.741 | 162 | 94 | 2 | 13 | 174 | 103 | 262 | 1.88e-34 | 123 |
| Msa1353320 | MtrunA17_Chr2g0287651 | 37.195 | 164 | 103 | 0 | 43 | 206 | 74 | 237 | 2.38e-33 | 125 |
| Msa1353320 | MtrunA17_Chr8g0389811 | 36.567 | 134 | 85 | 0 | 22 | 155 | 62 | 195 | 1.14e-29 | 109 |
| Msa1353320 | MtrunA17_Chr2g0331371 | 44.915 | 118 | 65 | 0 | 31 | 148 | 42 | 159 | 1.99e-28 | 106 |
| Msa1353320 | MtrunA17_Chr6g0473421 | 44.944 | 89 | 49 | 0 | 31 | 119 | 42 | 130 | 5.35e-23 | 90.5 |
| Msa1353320 | MtrunA17_Chr1g0172931 | 38.261 | 115 | 69 | 1 | 12 | 126 | 46 | 158 | 3.14e-21 | 85.9 |
| Msa1353320 | MtrunA17_Chr5g0448761 | 29.630 | 189 | 121 | 3 | 20 | 206 | 46 | 224 | 2.19e-17 | 80.1 |
| Msa1353320 | MtrunA17_Chr2g0290391 | 34.356 | 163 | 101 | 4 | 50 | 208 | 57 | 217 | 5.34e-16 | 75.9 |
| Msa1353320 | MtrunA17_Chr2g0276841 | 32.867 | 143 | 89 | 4 | 50 | 187 | 76 | 216 | 1.31e-15 | 74.7 |
| Msa1353320 | MtrunA17_Chr1g0198761 | 33.557 | 149 | 84 | 5 | 42 | 177 | 42 | 188 | 4.26e-15 | 73.2 |
| Msa1353320 | MtrunA17_Chr2g0294841 | 33.333 | 150 | 82 | 5 | 50 | 187 | 51 | 194 | 1.53e-13 | 68.9 |
| Msa1353320 | MtrunA17_Chr6g0458391 | 30.570 | 193 | 102 | 9 | 33 | 207 | 81 | 259 | 1.61e-13 | 68.6 |
| Msa1353320 | MtrunA17_Chr5g0431001 | 32.117 | 137 | 83 | 3 | 50 | 177 | 67 | 202 | 3.65e-13 | 67.4 |
| Msa1353320 | MtrunA17_Chr1g0202341 | 27.673 | 159 | 110 | 3 | 49 | 206 | 56 | 210 | 2.06e-12 | 65.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1353320 | AT5G28530.1 | 33.951 | 162 | 103 | 3 | 50 | 208 | 61 | 221 | 1.10e-19 | 87.0 |
| Msa1353320 | AT5G28530.3 | 33.951 | 162 | 103 | 3 | 50 | 208 | 61 | 221 | 1.13e-19 | 87.0 |
| Msa1353320 | AT5G28530.2 | 33.951 | 162 | 103 | 3 | 50 | 208 | 61 | 221 | 1.13e-19 | 87.0 |
| Msa1353320 | AT5G28530.4 | 33.951 | 162 | 103 | 3 | 50 | 208 | 61 | 221 | 1.20e-19 | 86.7 |
| Msa1353320 | AT5G28530.5 | 33.951 | 162 | 103 | 3 | 50 | 208 | 61 | 221 | 1.30e-19 | 86.7 |
| Msa1353320 | AT4G38180.1 | 30.986 | 213 | 136 | 7 | 3 | 206 | 26 | 236 | 2.35e-16 | 77.4 |
| Msa1353320 | AT1G10240.2 | 31.515 | 165 | 101 | 6 | 50 | 206 | 54 | 214 | 2.67e-15 | 74.3 |
| Msa1353320 | AT1G10240.1 | 31.515 | 165 | 101 | 6 | 50 | 206 | 54 | 214 | 2.67e-15 | 74.3 |
Find 50 sgRNAs with CRISPR-Local
Find 60 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GCAATTGTGTTTAACGATAT+AGG | 0.353545 | tig0013235:+5414 | Msa1353320:CDS |
| CGTTGCGTGGCTCACGCTTT+CGG | 0.364192 | tig0013235:-5665 | Msa1353320:intergenic |
| TGTTGGTTTGCTAGAATGTC+CGG | 0.368843 | tig0013235:+5528 | Msa1353320:CDS |
| TGCGTAAGGCTCAAATGATT+AGG | 0.377793 | tig0013235:+5559 | Msa1353320:CDS |
| GAAGATGGATTAGATGGTTC+AGG | 0.385947 | tig0013235:+5384 | Msa1353320:CDS |
| TGAAGGATTTAGAGAGGATA+GGG | 0.408657 | tig0013235:+5626 | Msa1353320:CDS |
| TGGCCATGGTACCGTACATT+TGG | 0.419221 | tig0013235:-5890 | Msa1353320:intergenic |
| TCTGTTGAAGATGGATTAGA+TGG | 0.422289 | tig0013235:+5378 | Msa1353320:CDS |
| GCGTAAGGCTCAAATGATTA+GGG | 0.441513 | tig0013235:+5560 | Msa1353320:CDS |
| TAGCAAACCAACAATAAAAC+AGG | 0.448653 | tig0013235:-5518 | Msa1353320:intergenic |
| GGCTCAAATGATTAGGGATC+GGG | 0.451003 | tig0013235:+5566 | Msa1353320:CDS |
| GAGGCAAATTCTGTTGAAGA+TGG | 0.463355 | tig0013235:+5369 | Msa1353320:CDS |
| CCACATCTTGTTTCGTTGCG+TGG | 0.468890 | tig0013235:-5678 | Msa1353320:intergenic |
| CGAAACAAGATGTGGATGTA+AGG | 0.474068 | tig0013235:+5686 | Msa1353320:CDS |
| GGTACCATGGCCAATGCATC+CGG | 0.478832 | tig0013235:+5900 | Msa1353320:CDS |
| TATCGTTAAACACAATTGCT+TGG | 0.487002 | tig0013235:-5410 | Msa1353320:intergenic |
| ATGTTGGGTGGGACTTTGTG+TGG | 0.491891 | tig0013235:+5786 | Msa1353320:CDS |
| GTCCGGGTTCTCTGTGCGTA+AGG | 0.507877 | tig0013235:+5545 | Msa1353320:CDS |
| AAGGATTTGTATAACCAAGT+TGG | 0.513872 | tig0013235:+5951 | Msa1353320:CDS |
| GATGAGTGAAGGTGATATTG+AGG | 0.514474 | tig0013235:+5830 | Msa1353320:CDS |
| GCCTCTTCTTCAGATTCGAC+CGG | 0.514599 | tig0013235:-5351 | Msa1353320:intergenic |
| ATTGAACGCAGTAGAAAAGC+AGG | 0.515481 | tig0013235:+5855 | Msa1353320:CDS |
| GTGAAAACCACCGGATGCAT+TGG | 0.516512 | tig0013235:-5910 | Msa1353320:intergenic |
| TGATCATAACCATGATATGT+TGG | 0.520526 | tig0013235:+5770 | Msa1353320:CDS |
| AAGTCCCACCCAACATATCA+TGG | 0.526054 | tig0013235:-5779 | Msa1353320:intergenic |
| GTGAAGGATTTAGAGAGGAT+AGG | 0.527849 | tig0013235:+5625 | Msa1353320:CDS |
| ATGGTACCGTACATTTGGTA+TGG | 0.543018 | tig0013235:-5885 | Msa1353320:intergenic |
| AGCCTTACGCACAGAGAACC+CGG | 0.546120 | tig0013235:-5547 | Msa1353320:intergenic |
| GAAGGATTTAGAGAGGATAG+GGG | 0.551534 | tig0013235:+5627 | Msa1353320:CDS |
| ACCAACCTTGTGAAAACCAC+CGG | 0.551580 | tig0013235:-5919 | Msa1353320:intergenic |
| GATCATAACCATGATATGTT+GGG | 0.552642 | tig0013235:+5771 | Msa1353320:CDS |
| AGGCTCAAATGATTAGGGAT+CGG | 0.556672 | tig0013235:+5565 | Msa1353320:CDS |
| TAGACATTGTTACAAAGCGT+TGG | 0.557208 | tig0013235:+5727 | Msa1353320:CDS |
| GTTGGTTTGCTAGAATGTCC+GGG | 0.563741 | tig0013235:+5529 | Msa1353320:CDS |
| GCCGGTCGAATCTGAAGAAG+AGG | 0.579413 | tig0013235:+5350 | Msa1353320:CDS |
| TTCATGTGAAGGATTTAGAG+AGG | 0.585894 | tig0013235:+5620 | Msa1353320:CDS |
| AAGGATTTAGAGAGGATAGG+GGG | 0.589938 | tig0013235:+5628 | Msa1353320:CDS |
| ATAAGACCATACCAAATGTA+CGG | 0.618674 | tig0013235:+5879 | Msa1353320:CDS |
| CATAACCATGATATGTTGGG+TGG | 0.621498 | tig0013235:+5774 | Msa1353320:CDS |
| TGATATAATGAAGTTTGACA+TGG | 0.626135 | tig0013235:+5437 | Msa1353320:CDS |
| ATAACCATGATATGTTGGGT+GGG | 0.626489 | tig0013235:+5775 | Msa1353320:CDS |
| ACCACCGGATGCATTGGCCA+TGG | 0.631660 | tig0013235:-5904 | Msa1353320:intergenic |
| TTTGCATTGATGATGTTGGA+AGG | 0.635495 | tig0013235:+5466 | Msa1353320:CDS |
| ATACCAAATGTACGGTACCA+TGG | 0.637363 | tig0013235:+5887 | Msa1353320:CDS |
| AAACCAACAATAAAACAGGT+AGG | 0.641669 | tig0013235:-5514 | Msa1353320:intergenic |
| ACCATGGCCAATGCATCCGG+TGG | 0.644970 | tig0013235:+5903 | Msa1353320:CDS |
| CCACGCAACGAAACAAGATG+TGG | 0.660406 | tig0013235:+5678 | Msa1353320:CDS |
| GATGGATTAGATGGTTCAGG+TGG | 0.668303 | tig0013235:+5387 | Msa1353320:CDS |
| TTGAACGCAGTAGAAAAGCA+GGG | 0.669009 | tig0013235:+5856 | Msa1353320:CDS |
| CAAATGATTAGGGATCGGGA+AGG | 0.746751 | tig0013235:+5570 | Msa1353320:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | TGATATAATGAAGTTTGACA+TGG | + | tig0013235:5437-5456 | Msa1353320:CDS | 25.0% |
| TATCGTTAAACACAATTGCT+TGG | - | tig0013235:5413-5432 | Msa1353320:intergenic | 30.0% | |
| GCAATTGTGTTTAACGATAT+AGG | + | tig0013235:5414-5433 | Msa1353320:CDS | 30.0% | |
| !!! | AAGTTTTGCATTGATGATGT+TGG | + | tig0013235:5462-5481 | Msa1353320:CDS | 30.0% |
| ! | AAGGTATGATTTTGCTAGTT+TGG | + | tig0013235:5485-5504 | Msa1353320:CDS | 30.0% |
| AAACCAACAATAAAACAGGT+AGG | - | tig0013235:5517-5536 | Msa1353320:intergenic | 30.0% | |
| TAGCAAACCAACAATAAAAC+AGG | - | tig0013235:5521-5540 | Msa1353320:intergenic | 30.0% | |
| ! | ACGTTTTTATGTTCATGTGA+AGG | + | tig0013235:5609-5628 | Msa1353320:CDS | 30.0% |
| TGATCATAACCATGATATGT+TGG | + | tig0013235:5770-5789 | Msa1353320:CDS | 30.0% | |
| GATCATAACCATGATATGTT+GGG | + | tig0013235:5771-5790 | Msa1353320:CDS | 30.0% | |
| ATAAGACCATACCAAATGTA+CGG | + | tig0013235:5879-5898 | Msa1353320:CDS | 30.0% | |
| !!! | CAAGGTTGGTTTTGTTAAAA+AGG | + | tig0013235:5932-5951 | Msa1353320:CDS | 30.0% |
| !! | TCTGTTGAAGATGGATTAGA+TGG | + | tig0013235:5378-5397 | Msa1353320:CDS | 35.0% |
| !! | TTTGCATTGATGATGTTGGA+AGG | + | tig0013235:5466-5485 | Msa1353320:CDS | 35.0% |
| !! | TTGCCTACCTGTTTTATTGT+TGG | + | tig0013235:5511-5530 | Msa1353320:CDS | 35.0% |
| TTCATGTGAAGGATTTAGAG+AGG | + | tig0013235:5620-5639 | Msa1353320:CDS | 35.0% | |
| TGAAGGATTTAGAGAGGATA+GGG | + | tig0013235:5626-5645 | Msa1353320:CDS | 35.0% | |
| TAGACATTGTTACAAAGCGT+TGG | + | tig0013235:5727-5746 | Msa1353320:CDS | 35.0% | |
| ATAACCATGATATGTTGGGT+GGG | + | tig0013235:5775-5794 | Msa1353320:CDS | 35.0% | |
| ! | TCACTCATCTTTTTGTATGC+AGG | - | tig0013235:5819-5838 | Msa1353320:intergenic | 35.0% |
| GCATACAAAAAGATGAGTGA+AGG | + | tig0013235:5819-5838 | Msa1353320:CDS | 35.0% | |
| GAGGCAAATTCTGTTGAAGA+TGG | + | tig0013235:5369-5388 | Msa1353320:CDS | 40.0% | |
| !! | GAAGATGGATTAGATGGTTC+AGG | + | tig0013235:5384-5403 | Msa1353320:CDS | 40.0% |
| !! | TGTTGGTTTGCTAGAATGTC+CGG | + | tig0013235:5528-5547 | Msa1353320:CDS | 40.0% |
| TGCGTAAGGCTCAAATGATT+AGG | + | tig0013235:5559-5578 | Msa1353320:CDS | 40.0% | |
| GCGTAAGGCTCAAATGATTA+GGG | + | tig0013235:5560-5579 | Msa1353320:CDS | 40.0% | |
| AGGCTCAAATGATTAGGGAT+CGG | + | tig0013235:5565-5584 | Msa1353320:CDS | 40.0% | |
| GTGAAGGATTTAGAGAGGAT+AGG | + | tig0013235:5625-5644 | Msa1353320:CDS | 40.0% | |
| GAAGGATTTAGAGAGGATAG+GGG | + | tig0013235:5627-5646 | Msa1353320:CDS | 40.0% | |
| AAGGATTTAGAGAGGATAGG+GGG | + | tig0013235:5628-5647 | Msa1353320:CDS | 40.0% | |
| CGAAACAAGATGTGGATGTA+AGG | + | tig0013235:5686-5705 | Msa1353320:CDS | 40.0% | |
| CATAACCATGATATGTTGGG+TGG | + | tig0013235:5774-5793 | Msa1353320:CDS | 40.0% | |
| GATGAGTGAAGGTGATATTG+AGG | + | tig0013235:5830-5849 | Msa1353320:CDS | 40.0% | |
| ATTGAACGCAGTAGAAAAGC+AGG | + | tig0013235:5855-5874 | Msa1353320:CDS | 40.0% | |
| TTGAACGCAGTAGAAAAGCA+GGG | + | tig0013235:5856-5875 | Msa1353320:CDS | 40.0% | |
| ATGGTACCGTACATTTGGTA+TGG | - | tig0013235:5888-5907 | Msa1353320:intergenic | 40.0% | |
| ATACCAAATGTACGGTACCA+TGG | + | tig0013235:5887-5906 | Msa1353320:CDS | 40.0% | |
| !! | GATGGATTAGATGGTTCAGG+TGG | + | tig0013235:5387-5406 | Msa1353320:CDS | 45.0% |
| !! | GTTGGTTTGCTAGAATGTCC+GGG | + | tig0013235:5529-5548 | Msa1353320:CDS | 45.0% |
| GGCTCAAATGATTAGGGATC+GGG | + | tig0013235:5566-5585 | Msa1353320:CDS | 45.0% | |
| CAAATGATTAGGGATCGGGA+AGG | + | tig0013235:5570-5589 | Msa1353320:CDS | 45.0% | |
| AGAGGATAGGGGGTTAACAA+GGG | + | tig0013235:5638-5657 | Msa1353320:CDS | 45.0% | |
| AAGTCCCACCCAACATATCA+TGG | - | tig0013235:5782-5801 | Msa1353320:intergenic | 45.0% | |
| ACCAACCTTGTGAAAACCAC+CGG | - | tig0013235:5922-5941 | Msa1353320:intergenic | 45.0% | |
| GCCTCTTCTTCAGATTCGAC+CGG | - | tig0013235:5354-5373 | Msa1353320:intergenic | 50.0% | |
| GAGAGGATAGGGGGTTAACA+AGG | + | tig0013235:5637-5656 | Msa1353320:CDS | 50.0% | |
| ! | CCACATCTTGTTTCGTTGCG+TGG | - | tig0013235:5681-5700 | Msa1353320:intergenic | 50.0% |
| CCACGCAACGAAACAAGATG+TGG | + | tig0013235:5678-5697 | Msa1353320:CDS | 50.0% | |
| ATGTTGGGTGGGACTTTGTG+TGG | + | tig0013235:5786-5805 | Msa1353320:CDS | 50.0% | |
| TGGCCATGGTACCGTACATT+TGG | - | tig0013235:5893-5912 | Msa1353320:intergenic | 50.0% | |
| GTGAAAACCACCGGATGCAT+TGG | - | tig0013235:5913-5932 | Msa1353320:intergenic | 50.0% | |
| ! | TGCATCCGGTGGTTTTCACA+AGG | + | tig0013235:5914-5933 | Msa1353320:CDS | 50.0% |
| ! | TCCGGTGGTTTTCACAAGGT+TGG | + | tig0013235:5918-5937 | Msa1353320:CDS | 50.0% |
| GCCGGTCGAATCTGAAGAAG+AGG | + | tig0013235:5350-5369 | Msa1353320:CDS | 55.0% | |
| AGCCTTACGCACAGAGAACC+CGG | - | tig0013235:5550-5569 | Msa1353320:intergenic | 55.0% | |
| GGTACCATGGCCAATGCATC+CGG | + | tig0013235:5900-5919 | Msa1353320:CDS | 55.0% | |
| GTCCGGGTTCTCTGTGCGTA+AGG | + | tig0013235:5545-5564 | Msa1353320:CDS | 60.0% | |
| CGTTGCGTGGCTCACGCTTT+CGG | - | tig0013235:5668-5687 | Msa1353320:intergenic | 60.0% | |
| ACCATGGCCAATGCATCCGG+TGG | + | tig0013235:5903-5922 | Msa1353320:CDS | 60.0% | |
| ! | ACCACCGGATGCATTGGCCA+TGG | - | tig0013235:5907-5926 | Msa1353320:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| tig0013235 | gene | 5348 | 5971 | 5348 | ID=Msa1353320;Name=Msa1353320 |
| tig0013235 | mRNA | 5348 | 5971 | 5348 | ID=Msa1353320-mRNA-1;Parent=Msa1353320;Name=Msa1353320-mRNA-1;_AED=0.18;_eAED=0.18;_QI=0|-1|0|1|-1|1|1|0|208 |
| tig0013235 | exon | 5348 | 5971 | 5348 | ID=Msa1353320-mRNA-1:exon:8605;Parent=Msa1353320-mRNA-1 |
| tig0013235 | CDS | 5348 | 5971 | 5348 | ID=Msa1353320-mRNA-1:cds;Parent=Msa1353320-mRNA-1 |
| Gene Sequence |
| Protein sequence |
