AlfalfaGEDB | Msa1353620

M. sativa cultivar zhongmu-4 / Msa1353620

KEGG: -
GO: GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006355 GO:0008150 GO:0009889 GO:0010468 GO:0010556 GO:0019219 GO:0019222 GO:0031323 GO:0031326 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043565 GO:0044424 GO:0044464 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0060255 GO:0065007 GO:0080090 GO:0097159 GO:0140110 GO:1901363 GO:1903506 GO:2000112 GO:2001141
PFAM: PF03514.17

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1353620	XP_003594263.1	90.516	601	49	4	1	596	1	598	0.0	1114

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1353620	sp\|Q9M0M5\|SCL13_ARATH	47.500	600	234	10	1	594	1	525	4.59e-180	522

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1353620	G7IPA4	90.516	601	49	4	1	596	1	598	0.0	1114

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TFs/TRs:

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Gene ID	Type	Classification
Msa1353620	TF	GRAS

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
No data available in table

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PPI

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1353620	MtrunA17_Chr2g0290241	94.145	427	25	0	170	596	1	427	0.0	851
Msa1353620	MtrunA17_Chr4g0076091	55.132	604	202	8	1	595	1	544	0.0	622
Msa1353620	MtrunA17_Chr5g0447691	47.769	605	244	13	1	594	1	544	0.0	530
Msa1353620	MtrunA17_Chr3g0101141	54.588	425	189	3	173	594	119	542	4.79e-170	494
Msa1353620	MtrunA17_Chr5g0445331	56.959	388	162	3	211	594	146	532	6.72e-160	468
Msa1353620	MtrunA17_Chr2g0319091	54.712	382	166	2	215	593	201	578	4.87e-148	439
Msa1353620	MtrunA17_Chr1g0159461	47.170	371	195	1	224	594	223	592	3.79e-127	386
Msa1353620	MtrunA17_Chr3g0125811	47.170	371	195	1	224	594	201	570	3.47e-123	375
Msa1353620	MtrunA17_Chr7g0224271	34.343	396	223	6	221	595	295	674	1.37e-70	240
Msa1353620	MtrunA17_Chr4g0031721	32.540	378	245	3	221	593	311	683	1.11e-64	224

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1353620	AT4G17230.1	47.333	600	235	10	1	594	1	525	6.92e-180	519
Msa1353620	AT4G17230.2	47.333	600	235	10	1	594	1	525	6.92e-180	519
Msa1353620	AT5G48150.1	55.349	430	187	4	167	594	64	490	1.83e-167	486
Msa1353620	AT5G48150.4	55.349	430	187	4	167	594	64	490	1.83e-167	486
Msa1353620	AT5G48150.2	55.349	430	187	4	167	594	64	490	1.83e-167	486
Msa1353620	AT5G48150.3	55.349	430	187	4	167	594	64	490	1.83e-167	486
Msa1353620	AT1G50600.3	52.349	447	202	5	150	594	89	526	2.71e-160	469
Msa1353620	AT2G04890.1	57.662	385	155	2	210	594	37	413	8.97e-160	463
Msa1353620	AT1G50600.1	52.349	447	202	5	150	594	160	597	1.20e-159	470
Msa1353620	AT1G50600.2	54.028	422	189	3	174	594	184	601	1.37e-159	470

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Find 111 sgRNAs with CRISPR-Local

Find 246 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAGCTCAATTTCTTTCATT+TGG	0.293718	tig0013888:-1014	Msa1353620:intergenic
AAATTGCACAAGGATCACAG+TGG	0.820097	tig0013888:+1649	Msa1353620:CDS
AACACAAATGTATCGCCGTT+AGG	0.563179	tig0013888:+949	Msa1353620:CDS
AAGAGCAAGGGTGGAATCAT+CGG	0.498333	tig0013888:+1452	Msa1353620:CDS
AAGATTTCAATAAAGATTAT+AGG	0.216624	tig0013888:+2324	Msa1353620:CDS
AAGGTACATCCGAATATGAT+TGG	0.489420	tig0013888:+1247	Msa1353620:CDS
AAGGTTAGGTGCTTACATGT+TGG	0.546985	tig0013888:+1422	Msa1353620:CDS
AATCATATTCGGATGTACCT+TGG	0.577241	tig0013888:-1245	Msa1353620:intergenic
AATGTTTGAATCAATTGATG+TGG	0.662206	tig0013888:+2097	Msa1353620:CDS
AATTTGAGACCAATCATATT+CGG	0.388708	tig0013888:-1256	Msa1353620:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
TCAAAGAACCAATGTTTCTT+TGG	+	tig0013888:771-790	Msa1353620:CDS	30.0%
TGAGAAAATTGTGGTGAAAA+AGG	-	tig0013888:913-932	Msa1353620:intergenic	30.0%
GAAAGAAATTGAGCTTTCAT+TGG	+	tig0013888:1020-1039	Msa1353620:CDS	30.0%
TGAATTAATGATTTCAGAGC+TGG	+	tig0013888:1137-1156	Msa1353620:CDS	30.0%
GAATTAATGATTTCAGAGCT+GGG	+	tig0013888:1138-1157	Msa1353620:CDS	30.0%
TGTTAAAATGTATGGATGTG+AGG	+	tig0013888:1842-1861	Msa1353620:CDS	30.0%
ATTCTCTTCTTATCATCTCT+AGG	-	tig0013888:2128-2147	Msa1353620:intergenic	30.0%
TCAAAGAACCAATGTTTCTT+TGG	+	tig0013888:771-790	Msa1353620:CDS	30.0%
TGAGAAAATTGTGGTGAAAA+AGG	-	tig0013888:913-932	Msa1353620:intergenic	30.0%
GAAAGAAATTGAGCTTTCAT+TGG	+	tig0013888:1020-1039	Msa1353620:CDS	30.0%

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Chromosome	Type	Strat	End	Strand	Name
tig0013888	gene	637	2427	637	ID=Msa1353620;Name=Msa1353620
tig0013888	mRNA	637	2427	637	ID=Msa1353620-mRNA-1;Parent=Msa1353620;Name=Msa1353620-mRNA-1;_AED=0.00;_eAED=0.00;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|596
tig0013888	exon	637	2427	637	ID=Msa1353620-mRNA-1:exon:8754;Parent=Msa1353620-mRNA-1
tig0013888	CDS	637	2427	637	ID=Msa1353620-mRNA-1:cds;Parent=Msa1353620-mRNA-1

Gene Sequence

>Msa1353620

ATGTATACTTCTCAAAAGAAACGTAGTTCAGCCGGTATACCGTTATACTACCAGCAACAGTCTTTGAGACAGATTGATTCAAACAACAATCGTTTCAAAACGTTGCAAATCAATTCAAATAATGGTAGTATCAGTCAAAGAACCAATGTTTCTTTGGAAACAAGCAACTGGGAGAAGCAATTTTGTACCTTGGAATCTTCTCAAGTAGCCAATGAATTCATAGCTTTTGATTCTCCTTCATCTTCTAGTGGTAATGCATCCAACACCAGGAATCCTTTTTCACCACAATTTTCTCACTATGACTCCTCTGACAACACAAATGTATCGCCGTTAGGTGTTTACTCTAGTGCGGACGATGGTAGCTTTGAATTGAAGCACCAAATGAAAGAAATTGAGCTTTCATTGGTAGGTACCGATATAGAAGTATTCGGCAGCTGTCTGTCCAACTCAAATGGTAGCCTTCACCAAGGTACATCTCAAGATAATGAGTTTCAAATTGATGAATTAATGATTTCAGAGCTGGGCTATAAGGAATCGGAGATTTCATTGTTAGGTCCCAATTCAGATATCATCGACAGCTGCCAGTCCAACTTAAACGGTAGCCTCCACCAAGGTACATCCGAATATGATTGGTCTCAAATTGTAGAAATGATTCCAAAGCTAGACTTGAAAGAAGAACTTATTAGATGTGCACAGTTTGTATTTGATGGTGATTTTCAAAAAGCAATAGGATTCATGAATAACGTGTTGGGAAAGATGGTATCGGTTGGCGGTTCTCCAATTCAAAGGTTAGGTGCTTACATGTTGGAGGGATTAAGAGCAAGGGTGGAATCATCGGGGAGTGCAATTTATAATGCGTTGAAATGTGAAGAACCAACAAGCAATGAGTTGATGAGTGCTATGCACACTTTGTATCAGATTTGCCCATATTTTCAATTTGCTTACATATCATCCAATGCTGTCATTTGTGAAGCAATGCAAAATGAATCAAGAATTCATATAATTGACTTCCAAATTGCACAAGGATCACAGTGGATGTTACTTTTGCATGCTCTCAAGCATAAACCTGGCGGACCTCCGTTCGTTCGTGTAACAGGTATCGATGATTCACAATCGTTTCATGCTAGAGGTGGAAGACTTGATATTGTAGGAAAAAAGCTAGAAGATTGTGCAAAAACTTGTAGAGTTCCATTTGAGTTCAATAGTGTTAAAATGTATGGATGTGAGGTTCAACTGGAAGATTTTGAAGTTCAACATGATGAAGTTCTTGCTGTGAATTTTCCTTTTGCTTTGCATCACATACCGGACGAGAGCGTGAGCATGGAGAATCATAGAGACCGGCTTTTGAGACTGGTGAAGATTTTGTCGCCTAAGGTCGTGTTATTTGTGGAGCAGGAGTCAAACACAAACACATCTCCATTTTTACCAAGGTTTGCTGAGACTTTAAGTTATTACACTGCAATGTTTGAATCAATTGATGTGGCTCTTCCTAGAGATGATAAGAAGAGAATTAATGCAGAACAACACTGTGTGGCTCGCGATATCGTTAACATCATAGCTTGTGAGGGAGATGAGAGAGTTGAAAGACATGAACTGTTTGGAAAATGGAGGAAAAGATTTTCAATGGCGGGTTTTGTACCGTTACCGTTGAGTACCTCGGTGATTGATTCAGTTAGAACTCTCTTGAAAGATTTCAATAAAGATTATAGGATTGAAGAAACAGATGTTGCTATCAATTTAGCGTGGAAGAGTAAAGTTATGTGCACATCTTCTGCTTGGAGATGTTATTGA

Protein sequence

>Msa1353620

MYTSQKKRSSAGIPLYYQQQSLRQIDSNNNRFKTLQINSNNGSISQRTNVSLETSNWEKQFCTLESSQVANEFIAFDSPSSSSGNASNTRNPFSPQFSHYDSSDNTNVSPLGVYSSADDGSFELKHQMKEIELSLVGTDIEVFGSCLSNSNGSLHQGTSQDNEFQIDELMISELGYKESEISLLGPNSDIIDSCQSNLNGSLHQGTSEYDWSQIVEMIPKLDLKEELIRCAQFVFDGDFQKAIGFMNNVLGKMVSVGGSPIQRLGAYMLEGLRARVESSGSAIYNALKCEEPTSNELMSAMHTLYQICPYFQFAYISSNAVICEAMQNESRIHIIDFQIAQGSQWMLLLHALKHKPGGPPFVRVTGIDDSQSFHARGGRLDIVGKKLEDCAKTCRVPFEFNSVKMYGCEVQLEDFEVQHDEVLAVNFPFALHHIPDESVSMENHRDRLLRLVKILSPKVVLFVEQESNTNTSPFLPRFAETLSYYTAMFESIDVALPRDDKKRINAEQHCVARDIVNIIACEGDERVERHELFGKWRKRFSMAGFVPLPLSTSVIDSVRTLLKDFNKDYRIEETDVAINLAWKSKVMCTSSAWRCY*