AlfalfaGEDB | Msa1355660

M. sativa cultivar zhongmu-4 / Msa1355660

KEGG: -
GO: -
PFAM: PF03101.18

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1355660	XP_039686443.1	94.958	119	6	0	7	125	1	119	1.57e-74	236

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1355660	sp\|Q9SZL8\|FRS5_ARATH	35.000	100	62	1	29	125	60	159	8.58e-11	62.0

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1355660	A0A072VK17	73.109	119	30	1	7	125	1	117	2.50e-51	168

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TFs/TRs:

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Gene ID	Type	Classification
Msa1355660	TF	FAR1

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
No data available in table

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PPI

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1355660	MtrunA17_Chr3g0082121	62.500	120	45	0	6	125	7	126	2.93e-43	150
Msa1355660	MtrunA17_Chr5g0401121	59.167	120	45	1	5	124	23	138	9.25e-38	126
Msa1355660	MtrunA17_Chr6g0458391	46.078	102	52	1	35	133	218	319	3.82e-22	91.3
Msa1355660	MtrunA17_Chr6g0458391	44.578	83	45	1	40	122	89	170	1.56e-15	72.4
Msa1355660	MtrunA17_Chr1g0203721	45.455	77	42	0	47	123	68	144	4.21e-18	79.7
Msa1355660	MtrunA17_Chr2g0329421	42.308	78	44	1	41	118	26	102	6.05e-13	63.2
Msa1355660	MtrunA17_Chr4g0033611	43.590	78	43	1	41	118	75	151	1.11e-12	63.2
Msa1355660	MtrunA17_Chr2g0324011	40.449	89	51	2	36	123	4	91	1.34e-12	63.9
Msa1355660	MtrunA17_Chr5g0448761	36.264	91	55	1	38	125	56	146	1.22e-11	61.2
Msa1355660	MtrunA17_Chr7g0258861	39.535	86	50	2	39	123	7	91	4.36e-11	59.3

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1355660	AT4G38180.1	35.000	100	62	1	29	125	60	159	8.73e-12	62.0
Msa1355660	AT3G59470.3	35.537	121	67	3	15	125	33	152	2.92e-11	59.7
Msa1355660	AT3G59470.1	35.537	121	67	3	15	125	33	152	2.92e-11	59.7
Msa1355660	AT3G59470.2	35.537	121	67	3	15	125	45	164	2.92e-11	59.7
Msa1355660	AT2G43280.1	40.476	84	49	1	40	122	20	103	3.34e-11	58.9

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Find 26 sgRNAs with CRISPR-Local

Find 35 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAATTCATAAGCATCTACCT+CGG	0.685544	tig0015000:+33932	None:intergenic
AACCGGGTGCAATGCACGTA+TGG	0.440531	tig0015000:-33742	Msa1355660:CDS
AATGAGTATAGTAAAAGAAT+TGG	0.336174	tig0015000:-33906	Msa1355660:CDS
ACAAAAGACGTCAACCGACT+GGG	0.550284	tig0015000:-33785	Msa1355660:CDS
ACCGGGTGCAATGCACGTAT+GGG	0.569580	tig0015000:-33741	Msa1355660:CDS
AGACAATTCTGTTGATAAAT+TGG	0.199895	tig0015000:-34039	Msa1355660:CDS
AGATTCACATGCTACAAAGA+AGG	0.592653	tig0015000:-33822	Msa1355660:CDS
ATCCAAGCGAGGTTTGATGT+CGG	0.664750	tig0015000:+33968	None:intergenic
CAAAAGACGTCAACCGACTG+GGG	0.676456	tig0015000:-33784	Msa1355660:CDS
CAATATTCTTGAACAAAATG+AGG	0.537039	tig0015000:-34012	Msa1355660:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAATTCATAAGCATCTACCT+CGG	+	tig0015000:34013-34032	None:intergenic	30.0%
TTTGATATTCGTCGAGAATA+TGG	-	tig0015000:34060-34079	Msa1355660:CDS	30.0%
TTGATATTCGTCGAGAATAT+GGG	-	tig0015000:34061-34080	Msa1355660:CDS	30.0%
GGGAATAAAAGCAAAAAAGA+CGG	-	tig0015000:34081-34100	Msa1355660:intron	30.0%
GGAATAAAAGCAAAAAAGAC+GGG	-	tig0015000:34082-34101	Msa1355660:intron	30.0%
GAATAAAAGCAAAAAAGACG+GGG	-	tig0015000:34083-34102	Msa1355660:intron	30.0%
GTGTATGTTTGTATTATGAG+TGG	-	tig0015000:34261-34280	Msa1355660:intron	30.0%
TTGTCTACCAAAATAGACGA+CGG	+	tig0015000:33889-33908	None:intergenic	35.0%
CTTGAACAAAATGAGGAACA+TGG	-	tig0015000:33937-33956	Msa1355660:CDS	35.0%
TCAAATATCATATCCAAGCG+AGG	+	tig0015000:33988-34007	None:intergenic	35.0%

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Chromosome	Type	Strat	End	Strand	Name
tig0015000	gene	33674	34290	33674	ID=Msa1355660;Name=Msa1355660
tig0015000	mRNA	33674	34290	33674	ID=Msa1355660-mRNA-1;Parent=Msa1355660;Name=Msa1355660-mRNA-1;_AED=0.32;_eAED=0.32;_QI=0\|0\|0\|0.33\|1\|1\|3\|0\|142
tig0015000	exon	34285	34290	34285	ID=Msa1355660-mRNA-1:exon:9595;Parent=Msa1355660-mRNA-1
tig0015000	exon	34201	34207	34201	ID=Msa1355660-mRNA-1:exon:9594;Parent=Msa1355660-mRNA-1
tig0015000	exon	33674	34089	33674	ID=Msa1355660-mRNA-1:exon:9593;Parent=Msa1355660-mRNA-1
tig0015000	CDS	34285	34290	34285	ID=Msa1355660-mRNA-1:cds;Parent=Msa1355660-mRNA-1
tig0015000	CDS	34201	34207	34201	ID=Msa1355660-mRNA-1:cds;Parent=Msa1355660-mRNA-1
tig0015000	CDS	33674	34089	33674	ID=Msa1355660-mRNA-1:cds;Parent=Msa1355660-mRNA-1

Gene Sequence

>Msa1355660

ATGGTATTTTTTGAAAGCATGAATCCGTCGTCTATTTTGGTAGACAATTCTGTTGATAAATTGGTAAACAATATTCTTGAACAAAATGAGGAACATGGAAAAGAAAGTATTGCCGACATCAAACCTCGCTTGGATATGATATTTGAATCCGAGGTAGATGCTTATGAATTTTATAATGAGTATAGTAAAAGAATTGGATTTGATATTCGTCGAGAATATGGGAATAAAAGCAAAAAAGACGGGGTTTTAACTTCAAGAAGATTCACATGCTACAAAGAAGGTACACGAAGTGTTGACAAAAGACGTCAACCGACTGGGGAGTCTACGGCAGAAACTAGAACCGGGTGCAATGCACGTATGGGCATTTCACTTGAAGTTGTTTACGTTAGTTTTTTTGAAGTGTATGTTTGTATTATGAGTGGGCAGTGA

Protein sequence

>Msa1355660

MVFFESMNPSSILVDNSVDKLVNNILEQNEEHGKESIADIKPRLDMIFESEVDAYEFYNEYSKRIGFDIRREYGNKSKKDGVLTSRRFTCYKEGTRSVDKRRQPTGESTAETRTGCNARMGISLEVVYVSFFEVYVCIMSGQ*