AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1359910

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1359910 MtrunA17_Chr4g0061801 96.341 164 4 1 1 162 1 164 2.73e-116 341
Msa1359910 MtrunA17_Chr7g0271951 44.697 132 71 2 32 162 426 556 6.25e-35 128
Msa1359910 MtrunA17_Chr8g0388341 34.416 154 73 8 31 162 33 180 1.72e-13 67.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1359910 AT3G05670.3 44.231 156 81 3 13 162 391 546 5.19e-36 132
Msa1359910 AT3G05670.1 44.231 156 81 3 13 162 391 546 5.19e-36 132
Msa1359910 AT3G05670.2 44.231 156 81 3 13 162 391 546 5.19e-36 132

Find 28 sgRNAs with CRISPR-Local

Find 224 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCTGCTCAGAAGACAAATT+CGG 0.369067 tig0016833:+1360 None:intergenic
CTTCGTTTGCATCATGGAAT+GGG 0.391668 tig0016833:-1155 Msa1359910:CDS
GCTTCGTTTGCATCATGGAA+TGG 0.409819 tig0016833:-1156 Msa1359910:CDS
TAAGATTCCTATTCGCGATC+AGG 0.423084 tig0016833:-1038 Msa1359910:intron
ACACCGTTCCTGAAGGTGAT+TGG 0.439694 tig0016833:-20 Msa1359910:CDS
TCTGCTCAGAAGACAAATTC+GGG 0.455964 tig0016833:+1361 None:intergenic
TACGAACGTTTGAGAATCTC+TGG 0.476070 tig0016833:+1101 None:intergenic
TTGCAGCAATCAATTTGTGC+AGG 0.486323 tig0016833:+1190 None:intergenic
GCAAATTCACGACCACCGTC+TGG 0.486453 tig0016833:-1385 Msa1359910:five_prime_UTR
AGAATGACCTGATCGCGAAT+AGG 0.488034 tig0016833:+1031 None:intergenic
TGGGCATCGAGATTCATGTT+TGG 0.492358 tig0016833:+1132 None:intergenic
AAATTCCACAGCAAACATCT+TGG 0.499946 tig0016833:+1239 None:intergenic
TTCTAGGTTTACCATCCTCA+TGG 0.502370 tig0016833:-180 Msa1359910:intron
GAGAATCTCTGGCGACAAAG+TGG 0.512531 tig0016833:+1112 None:intergenic
AAGAAAAGACATCATGCATA+GGG 0.555505 tig0016833:+1074 None:intergenic
GAAGAAAAGACATCATGCAT+AGG 0.556171 tig0016833:+1073 None:intergenic
TTGATGCGTTTGTTTGATGT+AGG 0.559764 tig0016833:+1304 None:intergenic
AGAATCTCTGGCGACAAAGT+GGG 0.566207 tig0016833:+1113 None:intergenic
CGCAAGTGTTGTATTTGCGA+TGG 0.582040 tig0016833:-120 Msa1359910:CDS
TGCAGCAATCAATTTGTGCA+GGG 0.587281 tig0016833:+1191 None:intergenic
TTGGGCTACACCGTTCCTGA+AGG 0.607395 tig0016833:-27 Msa1359910:CDS
AAGACAAATTCGGGACCAGA+CGG 0.632549 tig0016833:+1370 None:intergenic
CCTCATGGAAATATGACCAG+TGG 0.662971 tig0016833:-165 Msa1359910:CDS
AATAACCAAGATGTTTGCTG+TGG 0.680160 tig0016833:-1244 Msa1359910:CDS
TTTGTTTGATGTAGGCAACG+AGG 0.700541 tig0016833:+1312 None:intergenic
ACAAATTCGGGACCAGACGG+TGG 0.701984 tig0016833:+1373 None:intergenic
GGAATTTGCTACATCGAACG+CGG 0.706610 tig0016833:-1223 Msa1359910:CDS
AGGAATCTTAACATCCCGTG+AGG 0.714302 tig0016833:+1051 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAAATTAATAATGCACTAAT+TGG - tig0016833:798-817 Msa1359910:intron 15.0%
!! TAAATTAATAATGCACTAAT+TGG - tig0016833:798-817 Msa1359910:intron 15.0%
!!! TTTTTCACTATCATTAGTTT+AGG + tig0016833:617-636 None:intergenic 20.0%
!! AAGAAAAAAATGACAACTAT+CGG - tig0016833:821-840 Msa1359910:intron 20.0%
!! TAGATACATTTGACAATATT+TGG - tig0016833:922-941 Msa1359910:intron 20.0%
!! AGATACATTTGACAATATTT+GGG - tig0016833:923-942 Msa1359910:intron 20.0%
!!! ATAGAAGGAATAAAATTTTG+AGG - tig0016833:981-1000 Msa1359910:intron 20.0%
!!! TAGAAGGAATAAAATTTTGA+GGG - tig0016833:982-1001 Msa1359910:intron 20.0%
!! ATAGACAACAAAATTATGAA+AGG + tig0016833:1086-1105 None:intergenic 20.0%
!! AAAGATCACATATTAGTATA+AGG + tig0016833:1321-1340 None:intergenic 20.0%
!!! TTTTTCACTATCATTAGTTT+AGG + tig0016833:617-636 None:intergenic 20.0%
!! AAGAAAAAAATGACAACTAT+CGG - tig0016833:821-840 Msa1359910:intron 20.0%
!! TAGATACATTTGACAATATT+TGG - tig0016833:922-941 Msa1359910:intron 20.0%
!! AGATACATTTGACAATATTT+GGG - tig0016833:923-942 Msa1359910:intron 20.0%
!!! ATAGAAGGAATAAAATTTTG+AGG - tig0016833:981-1000 Msa1359910:intron 20.0%
!!! TAGAAGGAATAAAATTTTGA+GGG - tig0016833:982-1001 Msa1359910:intron 20.0%
!! ATAGACAACAAAATTATGAA+AGG + tig0016833:1086-1105 None:intergenic 20.0%
!! AAAGATCACATATTAGTATA+AGG + tig0016833:1321-1340 None:intergenic 20.0%
!! ATGCAATTTTGAATGTGTAA+CGG + tig0016833:400-419 None:intergenic 25.0%
! AGAAAAAAATGACAACTATC+GGG - tig0016833:822-841 Msa1359910:intron 25.0%
! ATATTGTCAAATGTATCTAG+TGG + tig0016833:921-940 None:intergenic 25.0%
! GATACATTTGACAATATTTG+GGG - tig0016833:924-943 Msa1359910:intron 25.0%
! TATATAGTAAAACACTCTTG+AGG - tig0016833:957-976 Msa1359910:intron 25.0%
!! AGAAGGAATAAAATTTTGAG+GGG - tig0016833:983-1002 Msa1359910:intron 25.0%
! GTCTATATATTATTTACTGC+TGG - tig0016833:1100-1119 Msa1359910:CDS 25.0%
! TAATGTTATAAGTAACCAAC+AGG - tig0016833:1166-1185 Msa1359910:CDS 25.0%
!! TAAAAAAATCATTAGCCTGT+TGG + tig0016833:1184-1203 None:intergenic 25.0%
!! ATGCAATTTTGAATGTGTAA+CGG + tig0016833:400-419 None:intergenic 25.0%
! AGAAAAAAATGACAACTATC+GGG - tig0016833:822-841 Msa1359910:intron 25.0%
! ATATTGTCAAATGTATCTAG+TGG + tig0016833:921-940 None:intergenic 25.0%
! GATACATTTGACAATATTTG+GGG - tig0016833:924-943 Msa1359910:intron 25.0%
! TATATAGTAAAACACTCTTG+AGG - tig0016833:957-976 Msa1359910:intron 25.0%
!! AGAAGGAATAAAATTTTGAG+GGG - tig0016833:983-1002 Msa1359910:intron 25.0%
! GTCTATATATTATTTACTGC+TGG - tig0016833:1100-1119 Msa1359910:CDS 25.0%
! TAATGTTATAAGTAACCAAC+AGG - tig0016833:1166-1185 Msa1359910:CDS 25.0%
!! TAAAAAAATCATTAGCCTGT+TGG + tig0016833:1184-1203 None:intergenic 25.0%
! TTCTTCTTCAGTTTTCACTT+CGG + tig0016833:124-143 None:intergenic 30.0%
TGCAAACGAAGCAAAAATAA+TGG + tig0016833:236-255 None:intergenic 30.0%
AAGAAAAGACATCATGCATA+GGG + tig0016833:329-348 None:intergenic 30.0%
TGTCAACAATTCATGTGTTA+GGG - tig0016833:498-517 Msa1359910:intron 30.0%
TTAGTTTAGGAATTCACTAG+CGG + tig0016833:604-623 None:intergenic 30.0%
ATCTTGACTGAAATTGTAGA+GGG - tig0016833:644-663 Msa1359910:intron 30.0%
TGAAGAACAAAGTCAAATCT+TGG - tig0016833:706-725 Msa1359910:intron 30.0%
! GTCTAAAATCTAAACATTCG+CGG + tig0016833:1040-1059 None:intergenic 30.0%
! TTCTTCTTCAGTTTTCACTT+CGG + tig0016833:124-143 None:intergenic 30.0%
TGCAAACGAAGCAAAAATAA+TGG + tig0016833:236-255 None:intergenic 30.0%
AAGAAAAGACATCATGCATA+GGG + tig0016833:329-348 None:intergenic 30.0%
TGTCAACAATTCATGTGTTA+GGG - tig0016833:498-517 Msa1359910:intron 30.0%
TTAGTTTAGGAATTCACTAG+CGG + tig0016833:604-623 None:intergenic 30.0%
ATCTTGACTGAAATTGTAGA+GGG - tig0016833:644-663 Msa1359910:intron 30.0%
TGAAGAACAAAGTCAAATCT+TGG - tig0016833:706-725 Msa1359910:intron 30.0%
! GTCTAAAATCTAAACATTCG+CGG + tig0016833:1040-1059 None:intergenic 30.0%
TTCTGCTCAGAAGACAAATT+CGG + tig0016833:43-62 None:intergenic 35.0%
TTGATGCGTTTGTTTGATGT+AGG + tig0016833:99-118 None:intergenic 35.0%
! AATAACCAAGATGTTTGCTG+TGG - tig0016833:156-175 Msa1359910:CDS 35.0%
AAATTCCACAGCAAACATCT+TGG + tig0016833:164-183 None:intergenic 35.0%
! TTTTTGCTTCGTTTGCATCA+TGG - tig0016833:239-258 Msa1359910:intron 35.0%
GAAGAAAAGACATCATGCAT+AGG + tig0016833:330-349 None:intergenic 35.0%
! ATGATGTCTTTTCTTCCTCA+CGG - tig0016833:334-353 Msa1359910:intron 35.0%
TCAAAATTGCATGAAACCCT+AGG - tig0016833:408-427 Msa1359910:intron 35.0%
AATGAAGTCTAGCTAACCTA+GGG + tig0016833:427-446 None:intergenic 35.0%
TAATGAAGTCTAGCTAACCT+AGG + tig0016833:428-447 None:intergenic 35.0%
! ATAAAAAAGAGCACACTCCT+AGG + tig0016833:454-473 None:intergenic 35.0%
!!! TGTGCTCTTTTTTATGTCTC+AGG - tig0016833:459-478 Msa1359910:intron 35.0%
!!! GTGCTCTTTTTTATGTCTCA+GGG - tig0016833:460-479 Msa1359910:intron 35.0%
CATGAATTGTTGACAGCTAT+GGG + tig0016833:493-512 None:intergenic 35.0%
ACATGAATTGTTGACAGCTA+TGG + tig0016833:494-513 None:intergenic 35.0%
CTGTCAACAATTCATGTGTT+AGG - tig0016833:497-516 Msa1359910:intron 35.0%
TAGTTTAGGAATTCACTAGC+GGG + tig0016833:603-622 None:intergenic 35.0%
GATCTTGACTGAAATTGTAG+AGG - tig0016833:643-662 Msa1359910:intron 35.0%
TCTTGACTGAAATTGTAGAG+GGG - tig0016833:645-664 Msa1359910:intron 35.0%
! AAAGTCAAATCTTGGTGTAG+TGG - tig0016833:714-733 Msa1359910:intron 35.0%
CGATTCCTCTCAAAAACTAT+CGG - tig0016833:847-866 Msa1359910:intron 35.0%
GATTCCTCTCAAAAACTATC+GGG - tig0016833:848-867 Msa1359910:intron 35.0%
TTGTCAAATGTATCTAGTGG+CGG + tig0016833:918-937 None:intergenic 35.0%
AAACACTCTTGAGGAATAGA+AGG - tig0016833:966-985 Msa1359910:intron 35.0%
!! TAAAATTTTGAGGGGAGACA+TGG - tig0016833:991-1010 Msa1359910:intron 35.0%
! GTTTCACATACGTTTTGTGT+TGG - tig0016833:1349-1368 Msa1359910:five_prime_UTR 35.0%
! ACATACGTTTTGTGTTGGAT+TGG - tig0016833:1354-1373 Msa1359910:five_prime_UTR 35.0%
! CATACGTTTTGTGTTGGATT+GGG - tig0016833:1355-1374 Msa1359910:five_prime_UTR 35.0%
TTCTGCTCAGAAGACAAATT+CGG + tig0016833:43-62 None:intergenic 35.0%
TTGATGCGTTTGTTTGATGT+AGG + tig0016833:99-118 None:intergenic 35.0%
! AATAACCAAGATGTTTGCTG+TGG - tig0016833:156-175 Msa1359910:CDS 35.0%
AAATTCCACAGCAAACATCT+TGG + tig0016833:164-183 None:intergenic 35.0%
! TTTTTGCTTCGTTTGCATCA+TGG - tig0016833:239-258 Msa1359910:intron 35.0%
GAAGAAAAGACATCATGCAT+AGG + tig0016833:330-349 None:intergenic 35.0%
! ATGATGTCTTTTCTTCCTCA+CGG - tig0016833:334-353 Msa1359910:intron 35.0%
TCAAAATTGCATGAAACCCT+AGG - tig0016833:408-427 Msa1359910:intron 35.0%
AATGAAGTCTAGCTAACCTA+GGG + tig0016833:427-446 None:intergenic 35.0%
TAATGAAGTCTAGCTAACCT+AGG + tig0016833:428-447 None:intergenic 35.0%
! ATAAAAAAGAGCACACTCCT+AGG + tig0016833:454-473 None:intergenic 35.0%
!!! TGTGCTCTTTTTTATGTCTC+AGG - tig0016833:459-478 Msa1359910:intron 35.0%
!!! GTGCTCTTTTTTATGTCTCA+GGG - tig0016833:460-479 Msa1359910:intron 35.0%
CATGAATTGTTGACAGCTAT+GGG + tig0016833:493-512 None:intergenic 35.0%
ACATGAATTGTTGACAGCTA+TGG + tig0016833:494-513 None:intergenic 35.0%
CTGTCAACAATTCATGTGTT+AGG - tig0016833:497-516 Msa1359910:intron 35.0%
TAGTTTAGGAATTCACTAGC+GGG + tig0016833:603-622 None:intergenic 35.0%
GATCTTGACTGAAATTGTAG+AGG - tig0016833:643-662 Msa1359910:intron 35.0%
TCTTGACTGAAATTGTAGAG+GGG - tig0016833:645-664 Msa1359910:intron 35.0%
! AAAGTCAAATCTTGGTGTAG+TGG - tig0016833:714-733 Msa1359910:intron 35.0%
CGATTCCTCTCAAAAACTAT+CGG - tig0016833:847-866 Msa1359910:intron 35.0%
GATTCCTCTCAAAAACTATC+GGG - tig0016833:848-867 Msa1359910:intron 35.0%
TTGTCAAATGTATCTAGTGG+CGG + tig0016833:918-937 None:intergenic 35.0%
AAACACTCTTGAGGAATAGA+AGG - tig0016833:966-985 Msa1359910:intron 35.0%
!! TAAAATTTTGAGGGGAGACA+TGG - tig0016833:991-1010 Msa1359910:intron 35.0%
! GTTTCACATACGTTTTGTGT+TGG - tig0016833:1349-1368 Msa1359910:five_prime_UTR 35.0%
! ACATACGTTTTGTGTTGGAT+TGG - tig0016833:1354-1373 Msa1359910:five_prime_UTR 35.0%
! CATACGTTTTGTGTTGGATT+GGG - tig0016833:1355-1374 Msa1359910:five_prime_UTR 35.0%
TCTGCTCAGAAGACAAATTC+GGG + tig0016833:42-61 None:intergenic 40.0%
TTTGTTTGATGTAGGCAACG+AGG + tig0016833:91-110 None:intergenic 40.0%
TGCAGCAATCAATTTGTGCA+GGG + tig0016833:212-231 None:intergenic 40.0%
TTGCAGCAATCAATTTGTGC+AGG + tig0016833:213-232 None:intergenic 40.0%
CTTCGTTTGCATCATGGAAT+GGG - tig0016833:245-264 Msa1359910:intron 40.0%
TACGAACGTTTGAGAATCTC+TGG + tig0016833:302-321 None:intergenic 40.0%
! TGATGTCTTTTCTTCCTCAC+GGG - tig0016833:335-354 Msa1359910:intron 40.0%
TAAGATTCCTATTCGCGATC+AGG - tig0016833:362-381 Msa1359910:intron 40.0%
GCTAGACTTCATTAAGACCT+AGG - tig0016833:434-453 Msa1359910:intron 40.0%
CAACAATTCATGTGTTAGGG+CGG - tig0016833:501-520 Msa1359910:intron 40.0%
ATTGAAGGCAAAGCTGTGTA+TGG + tig0016833:528-547 None:intergenic 40.0%
TACACAGCTTTGCCTTCAAT+TGG - tig0016833:528-547 Msa1359910:intron 40.0%
ACACAGCTTTGCCTTCAATT+GGG - tig0016833:529-548 Msa1359910:intron 40.0%
AGTTTAGGAATTCACTAGCG+GGG + tig0016833:602-621 None:intergenic 40.0%
TACTGCTGGTGATCAATGAA+CGG - tig0016833:1114-1133 Msa1359910:CDS 40.0%
TTCTAGGTTTACCATCCTCA+TGG - tig0016833:1220-1239 Msa1359910:CDS 40.0%
TGGTCATATTTCCATGAGGA+TGG + tig0016833:1234-1253 None:intergenic 40.0%
ATTAGAATCAACAGGACCAC+TGG + tig0016833:1254-1273 None:intergenic 40.0%
CGTTCAGCATTAGAATCAAC+AGG + tig0016833:1262-1281 None:intergenic 40.0%
AAACCAATCACCTTCAGGAA+CGG + tig0016833:1386-1405 None:intergenic 40.0%
TCTGCTCAGAAGACAAATTC+GGG + tig0016833:42-61 None:intergenic 40.0%
TTTGTTTGATGTAGGCAACG+AGG + tig0016833:91-110 None:intergenic 40.0%
TGCAGCAATCAATTTGTGCA+GGG + tig0016833:212-231 None:intergenic 40.0%
TTGCAGCAATCAATTTGTGC+AGG + tig0016833:213-232 None:intergenic 40.0%
CTTCGTTTGCATCATGGAAT+GGG - tig0016833:245-264 Msa1359910:intron 40.0%
TACGAACGTTTGAGAATCTC+TGG + tig0016833:302-321 None:intergenic 40.0%
! TGATGTCTTTTCTTCCTCAC+GGG - tig0016833:335-354 Msa1359910:intron 40.0%
TAAGATTCCTATTCGCGATC+AGG - tig0016833:362-381 Msa1359910:intron 40.0%
GCTAGACTTCATTAAGACCT+AGG - tig0016833:434-453 Msa1359910:intron 40.0%
CAACAATTCATGTGTTAGGG+CGG - tig0016833:501-520 Msa1359910:intron 40.0%
ATTGAAGGCAAAGCTGTGTA+TGG + tig0016833:528-547 None:intergenic 40.0%
TACACAGCTTTGCCTTCAAT+TGG - tig0016833:528-547 Msa1359910:intron 40.0%
ACACAGCTTTGCCTTCAATT+GGG - tig0016833:529-548 Msa1359910:intron 40.0%
AGTTTAGGAATTCACTAGCG+GGG + tig0016833:602-621 None:intergenic 40.0%
TACTGCTGGTGATCAATGAA+CGG - tig0016833:1114-1133 Msa1359910:CDS 40.0%
TTCTAGGTTTACCATCCTCA+TGG - tig0016833:1220-1239 Msa1359910:CDS 40.0%
TGGTCATATTTCCATGAGGA+TGG + tig0016833:1234-1253 None:intergenic 40.0%
ATTAGAATCAACAGGACCAC+TGG + tig0016833:1254-1273 None:intergenic 40.0%
CGTTCAGCATTAGAATCAAC+AGG + tig0016833:1262-1281 None:intergenic 40.0%
AAACCAATCACCTTCAGGAA+CGG + tig0016833:1386-1405 None:intergenic 40.0%
AAGACAAATTCGGGACCAGA+CGG + tig0016833:33-52 None:intergenic 45.0%
!! GGAATTTGCTACATCGAACG+CGG - tig0016833:177-196 Msa1359910:CDS 45.0%
GCTTCGTTTGCATCATGGAA+TGG - tig0016833:244-263 Msa1359910:intron 45.0%
!! TGGGCATCGAGATTCATGTT+TGG + tig0016833:271-290 None:intergenic 45.0%
AGAATCTCTGGCGACAAAGT+GGG + tig0016833:290-309 None:intergenic 45.0%
AGGAATCTTAACATCCCGTG+AGG + tig0016833:352-371 None:intergenic 45.0%
AGAATGACCTGATCGCGAAT+AGG + tig0016833:372-391 None:intergenic 45.0%
TTCATTTAACCTCACGTGCG+TGG - tig0016833:744-763 Msa1359910:intron 45.0%
!! CGATCCCGATAGTTTTTGAG+AGG + tig0016833:855-874 None:intergenic 45.0%
! GAGTCTGTTTAGCGAGTTGA+TGG + tig0016833:887-906 None:intergenic 45.0%
! AAATGTATCTAGTGGCGGAG+TGG + tig0016833:913-932 None:intergenic 45.0%
CCACTGGTCATATTTCCATG+AGG + tig0016833:1238-1257 None:intergenic 45.0%
CCTCATGGAAATATGACCAG+TGG - tig0016833:1235-1254 Msa1359910:CDS 45.0%
! CGCAAGTGTTGTATTTGCGA+TGG - tig0016833:1280-1299 Msa1359910:CDS 45.0%
AAGACAAATTCGGGACCAGA+CGG + tig0016833:33-52 None:intergenic 45.0%
!! GGAATTTGCTACATCGAACG+CGG - tig0016833:177-196 Msa1359910:CDS 45.0%
GCTTCGTTTGCATCATGGAA+TGG - tig0016833:244-263 Msa1359910:intron 45.0%
!! TGGGCATCGAGATTCATGTT+TGG + tig0016833:271-290 None:intergenic 45.0%
AGAATCTCTGGCGACAAAGT+GGG + tig0016833:290-309 None:intergenic 45.0%
AGGAATCTTAACATCCCGTG+AGG + tig0016833:352-371 None:intergenic 45.0%
AGAATGACCTGATCGCGAAT+AGG + tig0016833:372-391 None:intergenic 45.0%
TTCATTTAACCTCACGTGCG+TGG - tig0016833:744-763 Msa1359910:intron 45.0%
!! CGATCCCGATAGTTTTTGAG+AGG + tig0016833:855-874 None:intergenic 45.0%
! GAGTCTGTTTAGCGAGTTGA+TGG + tig0016833:887-906 None:intergenic 45.0%
! AAATGTATCTAGTGGCGGAG+TGG + tig0016833:913-932 None:intergenic 45.0%
CCACTGGTCATATTTCCATG+AGG + tig0016833:1238-1257 None:intergenic 45.0%
CCTCATGGAAATATGACCAG+TGG - tig0016833:1235-1254 Msa1359910:CDS 45.0%
! CGCAAGTGTTGTATTTGCGA+TGG - tig0016833:1280-1299 Msa1359910:CDS 45.0%
!! TAATACTTATTATAAATATA+TGG + tig0016833:673-692 None:intergenic 5.0%
!!! TTTTATTTTTTATATTTTCT+AGG - tig0016833:1204-1223 Msa1359910:CDS 5.0%
!! TAATACTTATTATAAATATA+TGG + tig0016833:673-692 None:intergenic 5.0%
!!! TTTTATTTTTTATATTTTCT+AGG - tig0016833:1204-1223 Msa1359910:CDS 5.0%
GAGAATCTCTGGCGACAAAG+TGG + tig0016833:291-310 None:intergenic 50.0%
CCACTAACGGTCCCAATTGA+AGG + tig0016833:543-562 None:intergenic 50.0%
CCTTCAATTGGGACCGTTAG+TGG - tig0016833:540-559 Msa1359910:intron 50.0%
CAATTGGGACCGTTAGTGGA+CGG - tig0016833:544-563 Msa1359910:intron 50.0%
ACATTCAAACCACGCACGTG+AGG + tig0016833:756-775 None:intergenic 50.0%
ACACCGTTCCTGAAGGTGAT+TGG - tig0016833:1380-1399 Msa1359910:five_prime_UTR 50.0%
GAGAATCTCTGGCGACAAAG+TGG + tig0016833:291-310 None:intergenic 50.0%
CCACTAACGGTCCCAATTGA+AGG + tig0016833:543-562 None:intergenic 50.0%
CCTTCAATTGGGACCGTTAG+TGG - tig0016833:540-559 Msa1359910:intron 50.0%
CAATTGGGACCGTTAGTGGA+CGG - tig0016833:544-563 Msa1359910:intron 50.0%
ACATTCAAACCACGCACGTG+AGG + tig0016833:756-775 None:intergenic 50.0%
ACACCGTTCCTGAAGGTGAT+TGG - tig0016833:1380-1399 Msa1359910:five_prime_UTR 50.0%
GCAAATTCACGACCACCGTC+TGG - tig0016833:15-34 Msa1359910:CDS 55.0%
ACAAATTCGGGACCAGACGG+TGG + tig0016833:30-49 None:intergenic 55.0%
CCAATCCCACCGTCCACTAA+CGG + tig0016833:556-575 None:intergenic 55.0%
!! GGATTGGTCCCCGGATTAGT+TGG - tig0016833:569-588 Msa1359910:intron 55.0%
GCACAGGACCAACTAATCCG+GGG + tig0016833:580-599 None:intergenic 55.0%
GGCACAGGACCAACTAATCC+GGG + tig0016833:581-600 None:intergenic 55.0%
GGGCACAGGACCAACTAATC+CGG + tig0016833:582-601 None:intergenic 55.0%
TTGGGCTACACCGTTCCTGA+AGG - tig0016833:1373-1392 Msa1359910:five_prime_UTR 55.0%
GCAAATTCACGACCACCGTC+TGG - tig0016833:15-34 Msa1359910:CDS 55.0%
ACAAATTCGGGACCAGACGG+TGG + tig0016833:30-49 None:intergenic 55.0%
CCAATCCCACCGTCCACTAA+CGG + tig0016833:556-575 None:intergenic 55.0%
!! GGATTGGTCCCCGGATTAGT+TGG - tig0016833:569-588 Msa1359910:intron 55.0%
GCACAGGACCAACTAATCCG+GGG + tig0016833:580-599 None:intergenic 55.0%
GGCACAGGACCAACTAATCC+GGG + tig0016833:581-600 None:intergenic 55.0%
GGGCACAGGACCAACTAATC+CGG + tig0016833:582-601 None:intergenic 55.0%
TTGGGCTACACCGTTCCTGA+AGG - tig0016833:1373-1392 Msa1359910:five_prime_UTR 55.0%
! TTGGGACCGTTAGTGGACGG+TGG - tig0016833:547-566 Msa1359910:intron 60.0%
! TGGGACCGTTAGTGGACGGT+GGG - tig0016833:548-567 Msa1359910:intron 60.0%
! CCGTTAGTGGACGGTGGGAT+TGG - tig0016833:553-572 Msa1359910:intron 60.0%
GGAATTCACTAGCGGGGCAC+AGG + tig0016833:596-615 None:intergenic 60.0%
GCACATCCCTGCTCGAGAGA+AGG - tig0016833:1013-1032 Msa1359910:intron 60.0%
! TTGGGACCGTTAGTGGACGG+TGG - tig0016833:547-566 Msa1359910:intron 60.0%
! TGGGACCGTTAGTGGACGGT+GGG - tig0016833:548-567 Msa1359910:intron 60.0%
! CCGTTAGTGGACGGTGGGAT+TGG - tig0016833:553-572 Msa1359910:intron 60.0%
GGAATTCACTAGCGGGGCAC+AGG + tig0016833:596-615 None:intergenic 60.0%
GCACATCCCTGCTCGAGAGA+AGG - tig0016833:1013-1032 Msa1359910:intron 60.0%
!! TGGACGGTGGGATTGGTCCC+CGG - tig0016833:560-579 Msa1359910:intron 65.0%
CGCGGACCTTCTCTCGAGCA+GGG + tig0016833:1022-1041 None:intergenic 65.0%
TCGCGGACCTTCTCTCGAGC+AGG + tig0016833:1023-1042 None:intergenic 65.0%
!! TGGACGGTGGGATTGGTCCC+CGG - tig0016833:560-579 Msa1359910:intron 65.0%
CGCGGACCTTCTCTCGAGCA+GGG + tig0016833:1022-1041 None:intergenic 65.0%
TCGCGGACCTTCTCTCGAGC+AGG + tig0016833:1023-1042 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
tig0016833 gene 14 1408 14 ID=Msa1359910;Name=Msa1359910
tig0016833 mRNA 14 1408 14 ID=Msa1359910-mRNA-1;Parent=Msa1359910;Name=Msa1359910-mRNA-1;_AED=0.00;_eAED=0.00;_QI=67|1|0.5|1|1|1|2|0|162
tig0016833 exon 14 196 14 ID=Msa1359910-mRNA-1:exon:11618;Parent=Msa1359910-mRNA-1
tig0016833 exon 1039 1408 1039 ID=Msa1359910-mRNA-1:exon:11617;Parent=Msa1359910-mRNA-1
tig0016833 five_prime_UTR 1342 1408 1342 ID=Msa1359910-mRNA-1:five_prime_utr;Parent=Msa1359910-mRNA-1
tig0016833 CDS 1039 1341 1039 ID=Msa1359910-mRNA-1:cds;Parent=Msa1359910-mRNA-1
tig0016833 CDS 14 196 14 ID=Msa1359910-mRNA-1:cds;Parent=Msa1359910-mRNA-1
Gene Sequence

>Msa1359910

ATGTCGACCTCGTTGCCTACATCAAACAAACGCATCAAAACCGAAGTGAAAACTGAAGAAGAAGACGACAATGACAATAACCAAGATGTTTGCTGTGGAATTTGCTACATCGAACGCGGAATTTCAGTCCCTGCACAAATTGATTGCTGCAACCATTATTTTTGCTTCGTTTGCATCATGGAATGGGCCAAACATGAATCTCGATGCCCACTTTGTCGCCAGAGATTCTCAAACGTTCGTAGACTCCCTATGCATGATGTCTTTTCTTCCTCACGGGATGTTAAGATTCCTATTCGCGATCAGGTTTACCATCCTCATGGAAATATGACCAGTGGTCCTGTTGATTCTAATGCTGAACGCAAGTGTTGTATTTGCGATGGTGCGAAAGATGAAAACCTTATACTAATATGTGATCTTTGTGATACTGTTTCACATACGTTTTGTGTTGGATTGGGCTACACCGTTCCTGAAGGTGATTGGTTTTGT
Protein sequence

>Msa1359910

MSTSLPTSNKRIKTEVKTEEEDDNDNNQDVCCGICYIERGISVPAQIDCCNHYFCFVCIMEWAKHESRCPLCRQRFSNVRRLPMHDVFSSSRDVKIPIRDQVYHPHGNMTSGPVDSNAERKCCICDGAKDENLILICDLCDTVSHTFCVGLGYTVPEGDWFC