AlfalfaGEDB | Msa1361920

M. sativa cultivar zhongmu-4 / Msa1361920

KEGG: -
GO: GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006355 GO:0006950 GO:0006970 GO:0006971 GO:0007154 GO:0008150 GO:0009267 GO:0009605 GO:0009628 GO:0009889 GO:0009891 GO:0009893 GO:0009987 GO:0009991 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0019219 GO:0019222 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031667 GO:0031668 GO:0031669 GO:0033554 GO:0042594 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043565 GO:0044424 GO:0044464 GO:0045893 GO:0045935 GO:0046982 GO:0046983 GO:0048518 GO:0048522 GO:0048580 GO:0048582 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051094 GO:0051171 GO:0051173 GO:0051239 GO:0051240 GO:0051252 GO:0051254 GO:0051716 GO:0060255 GO:0065007 GO:0071496 GO:0080050 GO:0080090 GO:0097159 GO:0140110 GO:1901363 GO:1902680 GO:1903506 GO:1903508 GO:2000026 GO:2000034 GO:2000112 GO:2000241 GO:2000243 GO:2000693 GO:2001141
PFAM: PF00170.24

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1361920	XP_003625968.1	82.781	151	12	2	1	151	1	137	5.84e-71	220

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1361920	sp\|Q9LZP8\|BZP53_ARATH	46.711	152	70	3	1	149	1	144	8.95e-34	119

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1361920	G7KST5	82.781	151	12	2	1	151	1	137	2.79e-71	220

TFs/TRs:

Gene ID	Type	Classification
Msa1361920	TF	bZIP

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1361920	MtrunA17_Chr7g0269951	82.781	151	12	2	1	151	1	137	5.37e-75	220
Msa1361920	MtrunA17_Chr1g0194681	52.055	146	60	3	1	140	1	142	8.20e-40	131
Msa1361920	MtrunA17_Chr4g0035414	48.387	93	47	1	10	101	17	109	8.71e-20	80.5
Msa1361920	MtrunA17_Chr3g0144941	53.012	83	39	0	19	101	27	109	8.88e-20	80.5
Msa1361920	MtrunA17_Chr1g0155551	54.217	83	38	0	19	101	27	109	1.06e-17	75.1
Msa1361920	MtrunA17_Chr8g0378901	40.594	101	55	1	10	105	18	118	7.03e-16	70.5
Msa1361920	MtrunA17_Chr5g0400901	52.857	70	24	1	2	62	32	101	4.14e-13	63.2
Msa1361920	MtrunA17_Chr4g0054291	39.823	113	40	3	7	94	45	154	5.74e-12	60.5
Msa1361920	MtrunA17_Chr7g0225661	47.887	71	22	1	19	74	81	151	8.47e-11	57.4

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1361920	AT3G62420.1	46.711	152	70	3	1	149	1	144	9.10e-35	119
Msa1361920	AT2G18160.1	44.118	102	52	1	18	119	27	123	1.10e-20	83.6
Msa1361920	AT1G75390.1	38.849	139	72	3	19	149	38	171	1.34e-20	83.2
Msa1361920	AT4G34590.1	48.810	84	43	0	19	102	24	107	1.36e-19	80.5
Msa1361920	AT1G75390.2	49.254	67	34	0	19	85	38	104	4.33e-12	59.7

Find 40 sgRNAs with CRISPR-Local

Find 42 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TTGTCCGAATCAAGCGATTA+AGG	0.283521	tig0017690:-15897	Msa1361920:CDS
GGCGATTGTTGGAGAACATT+AGG	0.296251	tig0017690:-16120	Msa1361920:CDS
CAGATTGGTTCTTGCATTGA+TGG	0.301394	tig0017690:-15862	None:intergenic
GGTTCAACGTGTGACGAGTT+CGG	0.348908	tig0017690:-16290	Msa1361920:CDS
GAGCGATGGACGAGAGAAAA+AGG	0.360427	tig0017690:-16249	Msa1361920:CDS
GTTCAACGTGTGACGAGTTC+GGG	0.369000	tig0017690:-16289	Msa1361920:CDS
TGAATCAATTTGAATGGCTT+CGG	0.385700	tig0017690:-16311	None:intergenic
GCGATTGTTGGAGAACATTA+GGG	0.409944	tig0017690:-16119	Msa1361920:CDS
CACGGGTTCAGTTGTGGATC+TGG	0.419548	tig0017690:+15929	None:intergenic
GTGATCGCCGTGCGGATTCA+CGG	0.422997	tig0017690:+16209	None:intergenic
ATCTTCAAGATGAGGCAAAC+AGG	0.431346	tig0017690:-16159	Msa1361920:CDS
AGTTGTGGATCTGGAATCTT+CGG	0.445106	tig0017690:+15938	None:intergenic
AAACGAGAATCGGCGATTGT+TGG	0.446153	tig0017690:-16131	Msa1361920:CDS
AATGCAAACGAAGCCGCTAA+TGG	0.446683	tig0017690:-16079	Msa1361920:CDS
AACATTAGGGCGAGGGAAGA+AGG	0.454886	tig0017690:-16106	Msa1361920:CDS
ACGTGTGACGAGTTCGGGTT+CGG	0.460336	tig0017690:-16284	Msa1361920:CDS
GACGCCTTAATCGCTTGATT+CGG	0.461919	tig0017690:+15893	None:intergenic
AGGCGTCTTCGTCTTCAGAT+TGG	0.488511	tig0017690:-15877	Msa1361920:CDS
TGTCCTCGGAGCTCGGATAC+AGG	0.488969	tig0017690:-16056	Msa1361920:CDS
AAAGCTGAGAGGGAAAAGAA+TGG	0.490064	tig0017690:-15977	Msa1361920:CDS
TCGGGTTCGGACGGTGTGAA+TGG	0.491849	tig0017690:-16271	Msa1361920:CDS
GTGAATCCGCACGGCGATCA+CGG	0.512811	tig0017690:-16207	Msa1361920:CDS
CTAATGGTGTCCTCGGAGCT+CGG	0.524737	tig0017690:-16063	Msa1361920:CDS
TGAATCCGCACGGCGATCAC+GGG	0.531059	tig0017690:-16206	Msa1361920:CDS
ACAGGTTGCGAAACGAGAAT+CGG	0.542037	tig0017690:-16141	Msa1361920:CDS
CCGCACGGCGATCACGGGAG+AGG	0.550454	tig0017690:-16201	Msa1361920:CDS
TGTTGGAGAACATTAGGGCG+AGG	0.554484	tig0017690:-16114	Msa1361920:CDS
ATCTGCCACGGGTTCAGTTG+TGG	0.561631	tig0017690:+15923	None:intergenic
AGCTCCGAGGACACCATTAG+CGG	0.564090	tig0017690:+16066	None:intergenic
GAAGCCGCTAATGGTGTCCT+CGG	0.572523	tig0017690:-16070	Msa1361920:CDS
TCGGACAAAGCATCTGCCAC+GGG	0.574386	tig0017690:+15912	None:intergenic
GTTGGAGAACATTAGGGCGA+GGG	0.586730	tig0017690:-16113	Msa1361920:CDS
GGAGAGGAAGCAGAAACTGA+TGG	0.591146	tig0017690:-16185	Msa1361920:CDS
TTCGGACAAAGCATCTGCCA+CGG	0.597740	tig0017690:+15911	None:intergenic
GGACGGTGTGAATGGAGCGA+TGG	0.618123	tig0017690:-16263	Msa1361920:CDS
GTGACGAGTTCGGGTTCGGA+CGG	0.621958	tig0017690:-16280	Msa1361920:CDS
GATGGAGGATCTTCAAGATG+AGG	0.635369	tig0017690:-16167	Msa1361920:CDS
CAGATCCACAACTGAACCCG+TGG	0.655694	tig0017690:-15928	Msa1361920:CDS
TTTCGAACCGTGAATCCGCA+CGG	0.677805	tig0017690:-16216	Msa1361920:CDS
GAGGAAGCAGAAACTGATGG+AGG	0.689697	tig0017690:-16182	Msa1361920:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	ATCTTCAAGATGAGGCAAAC+AGG	-	tig0017690:16004-16023	Msa1361920:CDS	40.0%
	GCGATTGTTGGAGAACATTA+GGG	-	tig0017690:16044-16063	Msa1361920:CDS	40.0%
	TGAGAGAGAAAAAGCTGAGA+GGG	-	tig0017690:16176-16195	Msa1361920:CDS	40.0%
	AAAGCTGAGAGGGAAAAGAA+TGG	-	tig0017690:16186-16205	Msa1361920:CDS	40.0%
!	AGTTGTGGATCTGGAATCTT+CGG	+	tig0017690:16228-16247	None:intergenic	40.0%
	TTGTCCGAATCAAGCGATTA+AGG	-	tig0017690:16266-16285	Msa1361920:CDS	40.0%
	GATGGAGGATCTTCAAGATG+AGG	-	tig0017690:15996-16015	Msa1361920:CDS	45.0%
	ACAGGTTGCGAAACGAGAAT+CGG	-	tig0017690:16022-16041	Msa1361920:CDS	45.0%
	AAACGAGAATCGGCGATTGT+TGG	-	tig0017690:16032-16051	Msa1361920:CDS	45.0%
	GGCGATTGTTGGAGAACATT+AGG	-	tig0017690:16043-16062	Msa1361920:CDS	45.0%
	AATGCAAACGAAGCCGCTAA+TGG	-	tig0017690:16084-16103	Msa1361920:CDS	45.0%
	CTGAGAGAGAAAAAGCTGAG+AGG	-	tig0017690:16175-16194	Msa1361920:CDS	45.0%
	GACGCCTTAATCGCTTGATT+CGG	+	tig0017690:16273-16292	None:intergenic	45.0%
	GGTTCAACGTGTGACGAGTT+CGG	-	tig0017690:15873-15892	Msa1361920:CDS	50.0%
	GTTCAACGTGTGACGAGTTC+GGG	-	tig0017690:15874-15893	Msa1361920:CDS	50.0%
	GAGCGATGGACGAGAGAAAA+AGG	-	tig0017690:15914-15933	Msa1361920:CDS	50.0%
	TTTCGAACCGTGAATCCGCA+CGG	-	tig0017690:15947-15966	Msa1361920:CDS	50.0%
	GGAGAGGAAGCAGAAACTGA+TGG	-	tig0017690:15978-15997	Msa1361920:CDS	50.0%
	GAGGAAGCAGAAACTGATGG+AGG	-	tig0017690:15981-16000	Msa1361920:CDS	50.0%
	TGTTGGAGAACATTAGGGCG+AGG	-	tig0017690:16049-16068	Msa1361920:CDS	50.0%
	GTTGGAGAACATTAGGGCGA+GGG	-	tig0017690:16050-16069	Msa1361920:CDS	50.0%
	AACATTAGGGCGAGGGAAGA+AGG	-	tig0017690:16057-16076	Msa1361920:CDS	50.0%
!	TTCGGACAAAGCATCTGCCA+CGG	+	tig0017690:16255-16274	None:intergenic	50.0%
	AGGCGTCTTCGTCTTCAGAT+TGG	-	tig0017690:16286-16305	Msa1361920:CDS	50.0%
!	ACGTGTGACGAGTTCGGGTT+CGG	-	tig0017690:15879-15898	Msa1361920:CDS	55.0%
	GAAGCCGCTAATGGTGTCCT+CGG	-	tig0017690:16093-16112	Msa1361920:CDS	55.0%
	AGCTCCGAGGACACCATTAG+CGG	+	tig0017690:16100-16119	None:intergenic	55.0%
	CTAATGGTGTCCTCGGAGCT+CGG	-	tig0017690:16100-16119	Msa1361920:CDS	55.0%
	ATTCCTGTATCCGAGCTCCG+AGG	+	tig0017690:16113-16132	None:intergenic	55.0%
	CACGGGTTCAGTTGTGGATC+TGG	+	tig0017690:16237-16256	None:intergenic	55.0%
	CAGATCCACAACTGAACCCG+TGG	-	tig0017690:16235-16254	Msa1361920:CDS	55.0%
	ATCTGCCACGGGTTCAGTTG+TGG	+	tig0017690:16243-16262	None:intergenic	55.0%
!	TCGGACAAAGCATCTGCCAC+GGG	+	tig0017690:16254-16273	None:intergenic	55.0%
!	GTGACGAGTTCGGGTTCGGA+CGG	-	tig0017690:15883-15902	Msa1361920:CDS	60.0%
	TCGGGTTCGGACGGTGTGAA+TGG	-	tig0017690:15892-15911	Msa1361920:CDS	60.0%
	GGACGGTGTGAATGGAGCGA+TGG	-	tig0017690:15900-15919	Msa1361920:CDS	60.0%
	GTGATCGCCGTGCGGATTCA+CGG	+	tig0017690:15957-15976	None:intergenic	60.0%
	GTGAATCCGCACGGCGATCA+CGG	-	tig0017690:15956-15975	Msa1361920:CDS	60.0%
	TGAATCCGCACGGCGATCAC+GGG	-	tig0017690:15957-15976	Msa1361920:CDS	60.0%
	TGTCCTCGGAGCTCGGATAC+AGG	-	tig0017690:16107-16126	Msa1361920:CDS	60.0%
	CCTCTCCCGTGATCGCCGTG+CGG	+	tig0017690:15965-15984	None:intergenic	70.0%
	CCGCACGGCGATCACGGGAG+AGG	-	tig0017690:15962-15981	Msa1361920:CDS	75.0%

Chromosome	Type	Strat	End	Strand	Name
tig0017690	gene	15865	16320	15865	ID=Msa1361920;Name=Msa1361920
tig0017690	mRNA	15865	16320	15865	ID=Msa1361920-mRNA-1;Parent=Msa1361920;Name=Msa1361920-mRNA-1;_AED=0.00;_eAED=0.00;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|151
tig0017690	exon	15865	16320	15865	ID=Msa1361920-mRNA-1:exon:12751;Parent=Msa1361920-mRNA-1
tig0017690	CDS	15865	16320	15865	ID=Msa1361920-mRNA-1:cds;Parent=Msa1361920-mRNA-1

Gene Sequence

>Msa1361920

ATGGCTTCGGTTCAACGTGTGACGAGTTCGGGTTCGGACGGTGTGAATGGAGCGATGGACGAGAGAAAAAGGAAGAGAATGATTTCGAACCGTGAATCCGCACGGCGATCACGGGAGAGGAAGCAGAAACTGATGGAGGATCTTCAAGATGAGGCAAACAGGTTGCGAAACGAGAATCGGCGATTGTTGGAGAACATTAGGGCGAGGGAAGAAGGTTTCAATGCAAACGAAGCCGCTAATGGTGTCCTCGGAGCTCGGATACAGGAATTGACAGATCAATTGAAGTTTCTGAACTCAATCATTGAAAAAGCTGAGAGAGAAAAAGCTGAGAGGGAAAAGAATGGATTTTCTGTTGAGATTCCGAAGATTCCAGATCCACAACTGAACCCGTGGCAGATGCTTTGTCCGAATCAAGCGATTAAGGCGTCTTCGTCTTCAGATTGGTTCTTGCATTGA

Protein sequence

>Msa1361920

MASVQRVTSSGSDGVNGAMDERKRKRMISNRESARRSRERKQKLMEDLQDEANRLRNENRRLLENIRAREEGFNANEAANGVLGARIQELTDQLKFLNSIIEKAEREKAEREKNGFSVEIPKIPDPQLNPWQMLCPNQAIKASSSSDWFLH*