AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1362650

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1362650 MtrunA17_Chr6g0452891 74.453 137 14 2 38 174 1 116 3.89e-64 196
Msa1362650 MtrunA17_Chr6g0452881 73.203 153 34 1 30 175 561 713 1.04e-63 209
Msa1362650 MtrunA17_Chr4g0037071 80.488 82 16 0 93 174 1 82 1.63e-43 144
Msa1362650 MtrunA17_Chr1g0179281 34.848 66 43 0 98 163 829 894 2.38e-11 61.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1362650 AT4G04885.1 63.000 100 37 0 76 175 591 690 6.54e-36 132
Msa1362650 AT1G66500.1 37.654 162 68 7 37 166 124 284 6.72e-23 94.4
Msa1362650 AT5G43620.1 37.342 158 68 7 37 166 123 277 4.18e-21 89.4

Find 75 sgRNAs with CRISPR-Local

Find 249 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGACGAACCTTGAGAATATT+TGG 0.171658 tig0017897:-69357 None:intergenic
GTGACTGCTGTGCTCATCTT+TGG 0.248435 tig0017897:-69451 None:intergenic
GGTTGTTGGCTTGACGTATT+AGG 0.261888 tig0017897:-69136 None:intergenic
AAGAGTTGTTTCCGATTCTT+TGG 0.310522 tig0017897:-69048 None:intergenic
ATGCCTGTTGTTATTCCATT+AGG 0.318753 tig0017897:-69765 None:intergenic
TCTTTGACTTCCTTCTTCAT+AGG 0.322887 tig0017897:-69999 None:intergenic
ATGCATTTAGCATGAATAAT+GGG 0.333668 tig0017897:-69807 None:intergenic
ATCGCATATATAGGTGTCTT+TGG 0.354576 tig0017897:+68424 Msa1362650:CDS
TATGTAATTTGTGATTTATA+TGG 0.358933 tig0017897:+69384 Msa1362650:CDS
CCGATTCTTTGGTATGGAAT+AGG 0.359303 tig0017897:-69037 None:intergenic
TATGCATTTAGCATGAATAA+TGG 0.373205 tig0017897:-69808 None:intergenic
TATATAGGTGTCTTTGGAAC+CGG 0.377723 tig0017897:+68430 Msa1362650:CDS
GTCTTTGGAACCGGGCTATT+AGG 0.379584 tig0017897:+68439 Msa1362650:CDS
GGAATAGGAAGTGGCATAAC+AGG 0.392358 tig0017897:-69022 None:intergenic
GGCATAACAGGTCGTGCATT+TGG 0.398786 tig0017897:-69010 None:intergenic
GTATATCTTAATGCGCCTAA+TGG 0.402277 tig0017897:+69750 Msa1362650:three_prime_UTR
TAACAGGTCGTGCATTTGGC+AGG 0.413669 tig0017897:-69006 None:intergenic
TGCCCAAGGTGTTATCTCAT+TGG 0.416501 tig0017897:+69195 Msa1362650:CDS
AAATTAGAATATCCAACAAT+TGG 0.418071 tig0017897:-69157 None:intergenic
AATATCCAACAATTGGTTGT+TGG 0.433148 tig0017897:-69150 None:intergenic
TTTGCAGTAATCGCATATAT+AGG 0.434152 tig0017897:+68415 None:intergenic
TTCCGACCTTTACCTCCAGC+TGG 0.442785 tig0017897:+69070 Msa1362650:CDS
GATTCGGATGAGGTAGAATC+TGG 0.448129 tig0017897:-69108 None:intergenic
AGTGAGACGATGTGGCTAAA+AGG 0.450066 tig0017897:+69546 Msa1362650:three_prime_UTR
ATTCTACCTCATCCGAATCT+TGG 0.451748 tig0017897:+69112 Msa1362650:CDS
TTGTTTCCGATTCTTTGGTA+TGG 0.469020 tig0017897:-69043 None:intergenic
AGTAGAGCATGTGGGCCAGC+TGG 0.471650 tig0017897:-69085 None:intergenic
TCAAGCCAACAACCAATTGT+TGG 0.475162 tig0017897:+69145 Msa1362650:CDS
TCACTGTAAAACACGTCAAA+AGG 0.480580 tig0017897:-69696 None:intergenic
CTTTGGTATGGAATAGGAAG+TGG 0.486169 tig0017897:-69031 None:intergenic
GCTAAAAGGTGCAGAGACAT+TGG 0.487532 tig0017897:+69560 Msa1362650:three_prime_UTR
CCTATTCCATACCAAAGAAT+CGG 0.490580 tig0017897:+69037 Msa1362650:CDS
GACGTATTAGGACCAAGATT+CGG 0.494335 tig0017897:-69124 None:intergenic
GCGCCTAATGGAATAACAAC+AGG 0.497466 tig0017897:+69762 Msa1362650:three_prime_UTR
TGTGGGCCAGCTGGAGGTAA+AGG 0.498674 tig0017897:-69076 None:intergenic
ATTGATTTAGTTACCTTCGC+AGG 0.505170 tig0017897:-69229 None:intergenic
CATCTTTGGATTTGAATCGA+TGG 0.508546 tig0017897:-69437 None:intergenic
TTGGGAGTTAAGTCAACTCC+AGG 0.511970 tig0017897:+69579 Msa1362650:three_prime_UTR
ACAGTGATGAAACGGAGGAT+TGG 0.518817 tig0017897:+69712 Msa1362650:three_prime_UTR
CCACAAGTTGTGCATTGTCT+AGG 0.520332 tig0017897:-69414 None:intergenic
CTAAAAGGTGCAGAGACATT+GGG 0.523678 tig0017897:+69561 Msa1362650:three_prime_UTR
AGAAAATAAAGATGAAGAGT+TGG 0.524053 tig0017897:+69626 Msa1362650:three_prime_UTR
CCTAGACAATGCACAACTTG+TGG 0.528311 tig0017897:+69414 Msa1362650:CDS
AGCACAGCAGTCACATGGAT+TGG 0.529243 tig0017897:+69460 Msa1362650:CDS
AGCCAATGAGATAACACCTT+GGG 0.530219 tig0017897:-69197 None:intergenic
TAAAATACATGTGCATTATG+TGG 0.538199 tig0017897:+69669 Msa1362650:three_prime_UTR
AACAGGTCGTGCATTTGGCA+GGG 0.540179 tig0017897:-69005 None:intergenic
AAAGAAAGCGCCTAATAGCC+CGG 0.541200 tig0017897:-68449 None:intergenic
TTGATTTAGTTACCTTCGCA+GGG 0.565255 tig0017897:-69228 None:intergenic
ATGCAGGGCACCTATGAAGA+AGG 0.565948 tig0017897:+69989 Msa1362650:intron
ACAGGTCGTGCATTTGGCAG+GGG 0.566169 tig0017897:-69004 None:intergenic
ATTAGTTCACTCATTGCCCA+AGG 0.573674 tig0017897:+69181 Msa1362650:CDS
GAATATTTGGATCAAACTCA+AGG 0.580223 tig0017897:-69344 None:intergenic
ATATAGGTGTCTTTGGAACC+GGG 0.582674 tig0017897:+68431 Msa1362650:CDS
AGATGAGCACAGCAGTCACA+TGG 0.587427 tig0017897:+69455 Msa1362650:CDS
GTTTGATCCAAATATTCTCA+AGG 0.588305 tig0017897:+69350 Msa1362650:CDS
TAATGGAATAACAACAGGCA+TGG 0.604751 tig0017897:+69767 Msa1362650:three_prime_UTR
AGAGCATGTGGGCCAGCTGG+AGG 0.611285 tig0017897:-69082 None:intergenic
CAGGTCGTGCATTTGGCAGG+GGG 0.612021 tig0017897:-69003 None:intergenic
GGCTAATCAAGCCCCTGCGA+AGG 0.612023 tig0017897:+69216 Msa1362650:CDS
GGCCAGCTGGAGGTAAAGGT+CGG 0.612329 tig0017897:-69072 None:intergenic
AGAATCTGGAGTAGAGCATG+TGG 0.640782 tig0017897:-69094 None:intergenic
TTAGGACCAAGATTCGGATG+AGG 0.644974 tig0017897:-69118 None:intergenic
GTTGGCAGTTCCGTTCGATG+AGG 0.647423 tig0017897:+69644 Msa1362650:three_prime_UTR
ACGGAGGATTGGATATGCAG+AGG 0.650743 tig0017897:+69723 Msa1362650:three_prime_UTR
TAGCCAATGAGATAACACCT+TGG 0.659744 tig0017897:-69198 None:intergenic
TGCATTTAGCATGAATAATG+GGG 0.666894 tig0017897:-69806 None:intergenic
TATACCGCAAAATCTTCACA+CGG 0.678850 tig0017897:-69843 None:intergenic
ATACCGCAAAATCTTCACAC+GGG 0.682049 tig0017897:-69842 None:intergenic
TGATTTAGTTACCTTCGCAG+GGG 0.687811 tig0017897:-69227 None:intergenic
GAATCTGGAGTAGAGCATGT+GGG 0.691664 tig0017897:-69093 None:intergenic
ACTCACAAACTACTTGCGAG+AGG 0.695457 tig0017897:-69526 None:intergenic
TACCGCAAAATCTTCACACG+GGG 0.732999 tig0017897:-69841 None:intergenic
AGTTTGTGAGTGAGACGATG+TGG 0.738502 tig0017897:+69538 Msa1362650:exon
GAAGTCAAAGAAAATGAACG+CGG 0.751197 tig0017897:+70011 Msa1362650:three_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTAAAATCATATTAACAACT+TGG - tig0017897:68539-68558 None:intergenic 15.0%
!!! ATTAATTCATTTGCAATTTT+TGG + tig0017897:68960-68979 Msa1362650:intron 15.0%
!!! TATGTAATTTGTGATTTATA+TGG + tig0017897:69384-69403 Msa1362650:CDS 15.0%
!! AACAAAAGAATAAATATTTC+CGG - tig0017897:69958-69977 None:intergenic 15.0%
!! TTAAAATCATATTAACAACT+TGG - tig0017897:68539-68558 None:intergenic 15.0%
!!! ATTAATTCATTTGCAATTTT+TGG + tig0017897:68960-68979 Msa1362650:intron 15.0%
!!! TATGTAATTTGTGATTTATA+TGG + tig0017897:69384-69403 Msa1362650:CDS 15.0%
!! AACAAAAGAATAAATATTTC+CGG - tig0017897:69958-69977 None:intergenic 15.0%
!! ATGTTTCTTGAACAAAAAAT+TGG - tig0017897:68476-68495 None:intergenic 20.0%
!!! AATATGATTTTAACTATGAG+TGG + tig0017897:68546-68565 Msa1362650:intron 20.0%
!!! ATATGATTTTAACTATGAGT+GGG + tig0017897:68547-68566 Msa1362650:intron 20.0%
!!! TTTTTTGAAGTAATTTCATG+TGG - tig0017897:68689-68708 None:intergenic 20.0%
!! AAATTAGAATATCCAACAAT+TGG - tig0017897:69160-69179 None:intergenic 20.0%
!! ACAGAATACAATAACAAAAT+TGG - tig0017897:69307-69326 None:intergenic 20.0%
!! ATGTTTCTTGAACAAAAAAT+TGG - tig0017897:68476-68495 None:intergenic 20.0%
!!! AATATGATTTTAACTATGAG+TGG + tig0017897:68546-68565 Msa1362650:intron 20.0%
!!! ATATGATTTTAACTATGAGT+GGG + tig0017897:68547-68566 Msa1362650:intron 20.0%
!!! TTTTTTGAAGTAATTTCATG+TGG - tig0017897:68689-68708 None:intergenic 20.0%
!! AAATTAGAATATCCAACAAT+TGG - tig0017897:69160-69179 None:intergenic 20.0%
!! ACAGAATACAATAACAAAAT+TGG - tig0017897:69307-69326 None:intergenic 20.0%
! ATCATATTAACAACTTGGAA+AGG - tig0017897:68534-68553 None:intergenic 25.0%
! TCATTTCGCAAAACATTAAA+AGG + tig0017897:68624-68643 Msa1362650:intron 25.0%
! CAAAACATTAAAAGGAAAGA+AGG + tig0017897:68632-68651 Msa1362650:intron 25.0%
! ATAACTTATCCAATCATACT+CGG - tig0017897:68910-68929 None:intergenic 25.0%
! TGTTATTGTATTCTGTTTGT+AGG + tig0017897:69311-69330 Msa1362650:intron 25.0%
!! TTCTGTTTGTAGGATTTTAT+TGG + tig0017897:69321-69340 Msa1362650:intron 25.0%
!!! CTCTTCATCTTTATTTTCTT+CGG - tig0017897:69625-69644 None:intergenic 25.0%
! AGAAAATAAAGATGAAGAGT+TGG + tig0017897:69626-69645 Msa1362650:three_prime_UTR 25.0%
! TAAAATACATGTGCATTATG+TGG + tig0017897:69669-69688 Msa1362650:three_prime_UTR 25.0%
! ATGCATTTAGCATGAATAAT+GGG - tig0017897:69810-69829 None:intergenic 25.0%
! TATGCATTTAGCATGAATAA+TGG - tig0017897:69811-69830 None:intergenic 25.0%
!! TTGTTTTCATCATTATATGC+AGG + tig0017897:69973-69992 Msa1362650:intron 25.0%
!! TGTTTTCATCATTATATGCA+GGG + tig0017897:69974-69993 Msa1362650:intron 25.0%
! ATCATATTAACAACTTGGAA+AGG - tig0017897:68534-68553 None:intergenic 25.0%
! TCATTTCGCAAAACATTAAA+AGG + tig0017897:68624-68643 Msa1362650:intron 25.0%
! CAAAACATTAAAAGGAAAGA+AGG + tig0017897:68632-68651 Msa1362650:intron 25.0%
! ATAACTTATCCAATCATACT+CGG - tig0017897:68910-68929 None:intergenic 25.0%
! TGTTATTGTATTCTGTTTGT+AGG + tig0017897:69311-69330 Msa1362650:intron 25.0%
!! TTCTGTTTGTAGGATTTTAT+TGG + tig0017897:69321-69340 Msa1362650:intron 25.0%
!!! CTCTTCATCTTTATTTTCTT+CGG - tig0017897:69625-69644 None:intergenic 25.0%
! AGAAAATAAAGATGAAGAGT+TGG + tig0017897:69626-69645 Msa1362650:three_prime_UTR 25.0%
! TAAAATACATGTGCATTATG+TGG + tig0017897:69669-69688 Msa1362650:three_prime_UTR 25.0%
! ATGCATTTAGCATGAATAAT+GGG - tig0017897:69810-69829 None:intergenic 25.0%
! TATGCATTTAGCATGAATAA+TGG - tig0017897:69811-69830 None:intergenic 25.0%
!! TTGTTTTCATCATTATATGC+AGG + tig0017897:69973-69992 Msa1362650:intron 25.0%
!! TGTTTTCATCATTATATGCA+GGG + tig0017897:69974-69993 Msa1362650:intron 25.0%
!! TTGTTGATGATGCCAATTAA+TGG + tig0017897:68575-68594 Msa1362650:intron 30.0%
!! TGTTGATGATGCCAATTAAT+GGG + tig0017897:68576-68595 Msa1362650:intron 30.0%
AAATGAAGACTTAGGATACT+TGG - tig0017897:68610-68629 None:intergenic 30.0%
ATCTATTCTGATGTTTCATG+TGG - tig0017897:68757-68776 None:intergenic 30.0%
TATAATCCATGGGAAAATTC+AGG - tig0017897:68804-68823 None:intergenic 30.0%
! TGATTCGGATTATAATCCAT+GGG - tig0017897:68814-68833 None:intergenic 30.0%
AAGTGAAGATGAAACTGATT+CGG - tig0017897:68829-68848 None:intergenic 30.0%
AATATCCAACAATTGGTTGT+TGG - tig0017897:69153-69172 None:intergenic 30.0%
GAATATTTGGATCAAACTCA+AGG - tig0017897:69347-69366 None:intergenic 30.0%
GTTTGATCCAAATATTCTCA+AGG + tig0017897:69350-69369 Msa1362650:CDS 30.0%
!! GCTTACAGTTTTTAGACATA+CGG - tig0017897:69500-69519 None:intergenic 30.0%
!!! TTCATCTTTATTTTCTTCGG+TGG - tig0017897:69622-69641 None:intergenic 30.0%
!!! GTATTTTAATCCTCATCGAA+CGG - tig0017897:69657-69676 None:intergenic 30.0%
TGCATTTAGCATGAATAATG+GGG - tig0017897:69809-69828 None:intergenic 30.0%
! AAGATTTTGCGGTATATGAA+GGG + tig0017897:69850-69869 Msa1362650:three_prime_UTR 30.0%
CATGATGTTATTATTGCAAG+TGG + tig0017897:69906-69925 Msa1362650:intron 30.0%
!! TTGTTGATGATGCCAATTAA+TGG + tig0017897:68575-68594 Msa1362650:intron 30.0%
!! TGTTGATGATGCCAATTAAT+GGG + tig0017897:68576-68595 Msa1362650:intron 30.0%
AAATGAAGACTTAGGATACT+TGG - tig0017897:68610-68629 None:intergenic 30.0%
ATCTATTCTGATGTTTCATG+TGG - tig0017897:68757-68776 None:intergenic 30.0%
TATAATCCATGGGAAAATTC+AGG - tig0017897:68804-68823 None:intergenic 30.0%
! TGATTCGGATTATAATCCAT+GGG - tig0017897:68814-68833 None:intergenic 30.0%
AAGTGAAGATGAAACTGATT+CGG - tig0017897:68829-68848 None:intergenic 30.0%
AATATCCAACAATTGGTTGT+TGG - tig0017897:69153-69172 None:intergenic 30.0%
GAATATTTGGATCAAACTCA+AGG - tig0017897:69347-69366 None:intergenic 30.0%
GTTTGATCCAAATATTCTCA+AGG + tig0017897:69350-69369 Msa1362650:CDS 30.0%
!! GCTTACAGTTTTTAGACATA+CGG - tig0017897:69500-69519 None:intergenic 30.0%
!!! TTCATCTTTATTTTCTTCGG+TGG - tig0017897:69622-69641 None:intergenic 30.0%
!!! GTATTTTAATCCTCATCGAA+CGG - tig0017897:69657-69676 None:intergenic 30.0%
TGCATTTAGCATGAATAATG+GGG - tig0017897:69809-69828 None:intergenic 30.0%
! AAGATTTTGCGGTATATGAA+GGG + tig0017897:69850-69869 Msa1362650:three_prime_UTR 30.0%
CATGATGTTATTATTGCAAG+TGG + tig0017897:69906-69925 Msa1362650:intron 30.0%
ATCGCATATATAGGTGTCTT+TGG + tig0017897:68424-68443 Msa1362650:CDS 35.0%
!! TATATAGGTGTCTTTGGAAC+CGG + tig0017897:68430-68449 Msa1362650:CDS 35.0%
CTTCAGTCACATAACTTACT+TGG - tig0017897:68504-68523 None:intergenic 35.0%
!! GTTGATGATGCCAATTAATG+GGG + tig0017897:68577-68596 Msa1362650:intron 35.0%
TGGAAATGAACCCCATTAAT+TGG - tig0017897:68590-68609 None:intergenic 35.0%
! GTTTTGCGAAATGAAGACTT+AGG - tig0017897:68618-68637 None:intergenic 35.0%
AAGGAAAGAAGGAAATCTAG+AGG + tig0017897:68643-68662 Msa1362650:intron 35.0%
! TGATTACCTGAATTTTCCCA+TGG + tig0017897:68795-68814 Msa1362650:intron 35.0%
!! CTGATTCGGATTATAATCCA+TGG - tig0017897:68815-68834 None:intergenic 35.0%
TATTTGACAGTCGAGATTAG+AGG + tig0017897:68879-68898 Msa1362650:intron 35.0%
TGGTGATCTTTGCAATAGTT+TGG + tig0017897:68980-68999 Msa1362650:intron 35.0%
CCTATTCCATACCAAAGAAT+CGG + tig0017897:69037-69056 Msa1362650:CDS 35.0%
TTGTTTCCGATTCTTTGGTA+TGG - tig0017897:69046-69065 None:intergenic 35.0%
! AAGAGTTGTTTCCGATTCTT+TGG - tig0017897:69051-69070 None:intergenic 35.0%
TTGATTTAGTTACCTTCGCA+GGG - tig0017897:69231-69250 None:intergenic 35.0%
! ATTGATTTAGTTACCTTCGC+AGG - tig0017897:69232-69251 None:intergenic 35.0%
ACGCAATCAAGTAAAATGGA+AGG - tig0017897:69283-69302 None:intergenic 35.0%
TGGTACGCAATCAAGTAAAA+TGG - tig0017897:69287-69306 None:intergenic 35.0%
TGACGAACCTTGAGAATATT+TGG - tig0017897:69360-69379 None:intergenic 35.0%
CATCTTTGGATTTGAATCGA+TGG - tig0017897:69440-69459 None:intergenic 35.0%
!! TTTATTTTCTTCGGTGGTCT+CGG - tig0017897:69616-69635 None:intergenic 35.0%
TCACTGTAAAACACGTCAAA+AGG - tig0017897:69699-69718 None:intergenic 35.0%
! CGTGTTTTACAGTGATGAAA+CGG + tig0017897:69704-69723 Msa1362650:three_prime_UTR 35.0%
GTATATCTTAATGCGCCTAA+TGG + tig0017897:69750-69769 Msa1362650:three_prime_UTR 35.0%
! ATGCCTGTTGTTATTCCATT+AGG - tig0017897:69768-69787 None:intergenic 35.0%
TAATGGAATAACAACAGGCA+TGG + tig0017897:69767-69786 Msa1362650:three_prime_UTR 35.0%
TATACCGCAAAATCTTCACA+CGG - tig0017897:69846-69865 None:intergenic 35.0%
! GAAGATTTTGCGGTATATGA+AGG + tig0017897:69849-69868 Msa1362650:three_prime_UTR 35.0%
! AGATTTTGCGGTATATGAAG+GGG + tig0017897:69851-69870 Msa1362650:three_prime_UTR 35.0%
! TCTTTGACTTCCTTCTTCAT+AGG - tig0017897:70002-70021 None:intergenic 35.0%
GAAGTCAAAGAAAATGAACG+CGG + tig0017897:70011-70030 Msa1362650:three_prime_UTR 35.0%
ATCGCATATATAGGTGTCTT+TGG + tig0017897:68424-68443 Msa1362650:CDS 35.0%
!! TATATAGGTGTCTTTGGAAC+CGG + tig0017897:68430-68449 Msa1362650:CDS 35.0%
CTTCAGTCACATAACTTACT+TGG - tig0017897:68504-68523 None:intergenic 35.0%
!! GTTGATGATGCCAATTAATG+GGG + tig0017897:68577-68596 Msa1362650:intron 35.0%
TGGAAATGAACCCCATTAAT+TGG - tig0017897:68590-68609 None:intergenic 35.0%
! GTTTTGCGAAATGAAGACTT+AGG - tig0017897:68618-68637 None:intergenic 35.0%
AAGGAAAGAAGGAAATCTAG+AGG + tig0017897:68643-68662 Msa1362650:intron 35.0%
! TGATTACCTGAATTTTCCCA+TGG + tig0017897:68795-68814 Msa1362650:intron 35.0%
!! CTGATTCGGATTATAATCCA+TGG - tig0017897:68815-68834 None:intergenic 35.0%
TATTTGACAGTCGAGATTAG+AGG + tig0017897:68879-68898 Msa1362650:intron 35.0%
TGGTGATCTTTGCAATAGTT+TGG + tig0017897:68980-68999 Msa1362650:intron 35.0%
CCTATTCCATACCAAAGAAT+CGG + tig0017897:69037-69056 Msa1362650:CDS 35.0%
TTGTTTCCGATTCTTTGGTA+TGG - tig0017897:69046-69065 None:intergenic 35.0%
! AAGAGTTGTTTCCGATTCTT+TGG - tig0017897:69051-69070 None:intergenic 35.0%
TTGATTTAGTTACCTTCGCA+GGG - tig0017897:69231-69250 None:intergenic 35.0%
! ATTGATTTAGTTACCTTCGC+AGG - tig0017897:69232-69251 None:intergenic 35.0%
ACGCAATCAAGTAAAATGGA+AGG - tig0017897:69283-69302 None:intergenic 35.0%
TGGTACGCAATCAAGTAAAA+TGG - tig0017897:69287-69306 None:intergenic 35.0%
TGACGAACCTTGAGAATATT+TGG - tig0017897:69360-69379 None:intergenic 35.0%
CATCTTTGGATTTGAATCGA+TGG - tig0017897:69440-69459 None:intergenic 35.0%
!! TTTATTTTCTTCGGTGGTCT+CGG - tig0017897:69616-69635 None:intergenic 35.0%
TCACTGTAAAACACGTCAAA+AGG - tig0017897:69699-69718 None:intergenic 35.0%
! CGTGTTTTACAGTGATGAAA+CGG + tig0017897:69704-69723 Msa1362650:three_prime_UTR 35.0%
GTATATCTTAATGCGCCTAA+TGG + tig0017897:69750-69769 Msa1362650:three_prime_UTR 35.0%
! ATGCCTGTTGTTATTCCATT+AGG - tig0017897:69768-69787 None:intergenic 35.0%
TAATGGAATAACAACAGGCA+TGG + tig0017897:69767-69786 Msa1362650:three_prime_UTR 35.0%
TATACCGCAAAATCTTCACA+CGG - tig0017897:69846-69865 None:intergenic 35.0%
! GAAGATTTTGCGGTATATGA+AGG + tig0017897:69849-69868 Msa1362650:three_prime_UTR 35.0%
! AGATTTTGCGGTATATGAAG+GGG + tig0017897:69851-69870 Msa1362650:three_prime_UTR 35.0%
! TCTTTGACTTCCTTCTTCAT+AGG - tig0017897:70002-70021 None:intergenic 35.0%
!! ATATAGGTGTCTTTGGAACC+GGG + tig0017897:68431-68450 Msa1362650:CDS 40.0%
CTTTGGTATGGAATAGGAAG+TGG - tig0017897:69034-69053 None:intergenic 40.0%
CCGATTCTTTGGTATGGAAT+AGG - tig0017897:69040-69059 None:intergenic 40.0%
ATTCTACCTCATCCGAATCT+TGG + tig0017897:69112-69131 Msa1362650:CDS 40.0%
GACGTATTAGGACCAAGATT+CGG - tig0017897:69127-69146 None:intergenic 40.0%
TCAAGCCAACAACCAATTGT+TGG + tig0017897:69145-69164 Msa1362650:CDS 40.0%
ATTAGTTCACTCATTGCCCA+AGG + tig0017897:69181-69200 Msa1362650:CDS 40.0%
AGCCAATGAGATAACACCTT+GGG - tig0017897:69200-69219 None:intergenic 40.0%
TAGCCAATGAGATAACACCT+TGG - tig0017897:69201-69220 None:intergenic 40.0%
TGATTTAGTTACCTTCGCAG+GGG - tig0017897:69230-69249 None:intergenic 40.0%
! CTAAAAGGTGCAGAGACATT+GGG + tig0017897:69561-69580 Msa1362650:three_prime_UTR 40.0%
! GTTTTACAGTGATGAAACGG+AGG + tig0017897:69707-69726 Msa1362650:three_prime_UTR 40.0%
ATACCGCAAAATCTTCACAC+GGG - tig0017897:69845-69864 None:intergenic 40.0%
!! AAGTGGACTTTTGCATATGC+TGG + tig0017897:69923-69942 Msa1362650:intron 40.0%
!! ATATAGGTGTCTTTGGAACC+GGG + tig0017897:68431-68450 Msa1362650:CDS 40.0%
CTTTGGTATGGAATAGGAAG+TGG - tig0017897:69034-69053 None:intergenic 40.0%
CCGATTCTTTGGTATGGAAT+AGG - tig0017897:69040-69059 None:intergenic 40.0%
ATTCTACCTCATCCGAATCT+TGG + tig0017897:69112-69131 Msa1362650:CDS 40.0%
GACGTATTAGGACCAAGATT+CGG - tig0017897:69127-69146 None:intergenic 40.0%
TCAAGCCAACAACCAATTGT+TGG + tig0017897:69145-69164 Msa1362650:CDS 40.0%
ATTAGTTCACTCATTGCCCA+AGG + tig0017897:69181-69200 Msa1362650:CDS 40.0%
AGCCAATGAGATAACACCTT+GGG - tig0017897:69200-69219 None:intergenic 40.0%
TAGCCAATGAGATAACACCT+TGG - tig0017897:69201-69220 None:intergenic 40.0%
TGATTTAGTTACCTTCGCAG+GGG - tig0017897:69230-69249 None:intergenic 40.0%
! CTAAAAGGTGCAGAGACATT+GGG + tig0017897:69561-69580 Msa1362650:three_prime_UTR 40.0%
! GTTTTACAGTGATGAAACGG+AGG + tig0017897:69707-69726 Msa1362650:three_prime_UTR 40.0%
ATACCGCAAAATCTTCACAC+GGG - tig0017897:69845-69864 None:intergenic 40.0%
!! AAGTGGACTTTTGCATATGC+TGG + tig0017897:69923-69942 Msa1362650:intron 40.0%
!! AAAGAAAGCGCCTAATAGCC+CGG - tig0017897:68452-68471 None:intergenic 45.0%
!! GAGGAGTATCCGAGTATGAT+TGG + tig0017897:68898-68917 Msa1362650:intron 45.0%
GGAATAGGAAGTGGCATAAC+AGG - tig0017897:69025-69044 None:intergenic 45.0%
! GAATCTGGAGTAGAGCATGT+GGG - tig0017897:69096-69115 None:intergenic 45.0%
! AGAATCTGGAGTAGAGCATG+TGG - tig0017897:69097-69116 None:intergenic 45.0%
GATTCGGATGAGGTAGAATC+TGG - tig0017897:69111-69130 None:intergenic 45.0%
TTAGGACCAAGATTCGGATG+AGG - tig0017897:69121-69140 None:intergenic 45.0%
!! GGTTGTTGGCTTGACGTATT+AGG - tig0017897:69139-69158 None:intergenic 45.0%
! TGCCCAAGGTGTTATCTCAT+TGG + tig0017897:69195-69214 Msa1362650:CDS 45.0%
CCACAAGTTGTGCATTGTCT+AGG - tig0017897:69417-69436 None:intergenic 45.0%
CCTAGACAATGCACAACTTG+TGG + tig0017897:69414-69433 Msa1362650:CDS 45.0%
ACTCACAAACTACTTGCGAG+AGG - tig0017897:69529-69548 None:intergenic 45.0%
AGTTTGTGAGTGAGACGATG+TGG + tig0017897:69538-69557 Msa1362650:exon 45.0%
AGTGAGACGATGTGGCTAAA+AGG + tig0017897:69546-69565 Msa1362650:three_prime_UTR 45.0%
! GCTAAAAGGTGCAGAGACAT+TGG + tig0017897:69560-69579 Msa1362650:three_prime_UTR 45.0%
TTGGGAGTTAAGTCAACTCC+AGG + tig0017897:69579-69598 Msa1362650:three_prime_UTR 45.0%
ACAGTGATGAAACGGAGGAT+TGG + tig0017897:69712-69731 Msa1362650:three_prime_UTR 45.0%
GCGCCTAATGGAATAACAAC+AGG + tig0017897:69762-69781 Msa1362650:three_prime_UTR 45.0%
! TACCCCGTGTGAAGATTTTG+CGG + tig0017897:69839-69858 Msa1362650:three_prime_UTR 45.0%
TACCGCAAAATCTTCACACG+GGG - tig0017897:69844-69863 None:intergenic 45.0%
CATATGCTGGTGTTTCAAGC+CGG + tig0017897:69936-69955 Msa1362650:intron 45.0%
!! AAAGAAAGCGCCTAATAGCC+CGG - tig0017897:68452-68471 None:intergenic 45.0%
!! GAGGAGTATCCGAGTATGAT+TGG + tig0017897:68898-68917 Msa1362650:intron 45.0%
GGAATAGGAAGTGGCATAAC+AGG - tig0017897:69025-69044 None:intergenic 45.0%
! GAATCTGGAGTAGAGCATGT+GGG - tig0017897:69096-69115 None:intergenic 45.0%
! AGAATCTGGAGTAGAGCATG+TGG - tig0017897:69097-69116 None:intergenic 45.0%
GATTCGGATGAGGTAGAATC+TGG - tig0017897:69111-69130 None:intergenic 45.0%
TTAGGACCAAGATTCGGATG+AGG - tig0017897:69121-69140 None:intergenic 45.0%
!! GGTTGTTGGCTTGACGTATT+AGG - tig0017897:69139-69158 None:intergenic 45.0%
! TGCCCAAGGTGTTATCTCAT+TGG + tig0017897:69195-69214 Msa1362650:CDS 45.0%
CCACAAGTTGTGCATTGTCT+AGG - tig0017897:69417-69436 None:intergenic 45.0%
CCTAGACAATGCACAACTTG+TGG + tig0017897:69414-69433 Msa1362650:CDS 45.0%
ACTCACAAACTACTTGCGAG+AGG - tig0017897:69529-69548 None:intergenic 45.0%
AGTTTGTGAGTGAGACGATG+TGG + tig0017897:69538-69557 Msa1362650:exon 45.0%
AGTGAGACGATGTGGCTAAA+AGG + tig0017897:69546-69565 Msa1362650:three_prime_UTR 45.0%
! GCTAAAAGGTGCAGAGACAT+TGG + tig0017897:69560-69579 Msa1362650:three_prime_UTR 45.0%
TTGGGAGTTAAGTCAACTCC+AGG + tig0017897:69579-69598 Msa1362650:three_prime_UTR 45.0%
ACAGTGATGAAACGGAGGAT+TGG + tig0017897:69712-69731 Msa1362650:three_prime_UTR 45.0%
GCGCCTAATGGAATAACAAC+AGG + tig0017897:69762-69781 Msa1362650:three_prime_UTR 45.0%
! TACCCCGTGTGAAGATTTTG+CGG + tig0017897:69839-69858 Msa1362650:three_prime_UTR 45.0%
TACCGCAAAATCTTCACACG+GGG - tig0017897:69844-69863 None:intergenic 45.0%
CATATGCTGGTGTTTCAAGC+CGG + tig0017897:69936-69955 Msa1362650:intron 45.0%
!! GTCTTTGGAACCGGGCTATT+AGG + tig0017897:68439-68458 Msa1362650:CDS 50.0%
AACAGGTCGTGCATTTGGCA+GGG - tig0017897:69008-69027 None:intergenic 50.0%
TAACAGGTCGTGCATTTGGC+AGG - tig0017897:69009-69028 None:intergenic 50.0%
GGCATAACAGGTCGTGCATT+TGG - tig0017897:69013-69032 None:intergenic 50.0%
GTGACTGCTGTGCTCATCTT+TGG - tig0017897:69454-69473 None:intergenic 50.0%
AGATGAGCACAGCAGTCACA+TGG + tig0017897:69455-69474 Msa1362650:CDS 50.0%
AGCACAGCAGTCACATGGAT+TGG + tig0017897:69460-69479 Msa1362650:CDS 50.0%
GTCTCGGTTGGCAAAAAACC+TGG - tig0017897:69600-69619 None:intergenic 50.0%
! TTTTCTTCGGTGGTCTCGGT+TGG - tig0017897:69612-69631 None:intergenic 50.0%
ACGGAGGATTGGATATGCAG+AGG + tig0017897:69723-69742 Msa1362650:three_prime_UTR 50.0%
! ATGCAGGGCACCTATGAAGA+AGG + tig0017897:69989-70008 Msa1362650:intron 50.0%
!! GTCTTTGGAACCGGGCTATT+AGG + tig0017897:68439-68458 Msa1362650:CDS 50.0%
AACAGGTCGTGCATTTGGCA+GGG - tig0017897:69008-69027 None:intergenic 50.0%
TAACAGGTCGTGCATTTGGC+AGG - tig0017897:69009-69028 None:intergenic 50.0%
GGCATAACAGGTCGTGCATT+TGG - tig0017897:69013-69032 None:intergenic 50.0%
GTGACTGCTGTGCTCATCTT+TGG - tig0017897:69454-69473 None:intergenic 50.0%
AGATGAGCACAGCAGTCACA+TGG + tig0017897:69455-69474 Msa1362650:CDS 50.0%
AGCACAGCAGTCACATGGAT+TGG + tig0017897:69460-69479 Msa1362650:CDS 50.0%
GTCTCGGTTGGCAAAAAACC+TGG - tig0017897:69600-69619 None:intergenic 50.0%
! TTTTCTTCGGTGGTCTCGGT+TGG - tig0017897:69612-69631 None:intergenic 50.0%
ACGGAGGATTGGATATGCAG+AGG + tig0017897:69723-69742 Msa1362650:three_prime_UTR 50.0%
! ATGCAGGGCACCTATGAAGA+AGG + tig0017897:69989-70008 Msa1362650:intron 50.0%
ACAGGTCGTGCATTTGGCAG+GGG - tig0017897:69007-69026 None:intergenic 55.0%
TTCCGACCTTTACCTCCAGC+TGG + tig0017897:69070-69089 Msa1362650:CDS 55.0%
GTTGGCAGTTCCGTTCGATG+AGG + tig0017897:69644-69663 Msa1362650:three_prime_UTR 55.0%
ACAGGTCGTGCATTTGGCAG+GGG - tig0017897:69007-69026 None:intergenic 55.0%
TTCCGACCTTTACCTCCAGC+TGG + tig0017897:69070-69089 Msa1362650:CDS 55.0%
GTTGGCAGTTCCGTTCGATG+AGG + tig0017897:69644-69663 Msa1362650:three_prime_UTR 55.0%
CAGGTCGTGCATTTGGCAGG+GGG - tig0017897:69006-69025 None:intergenic 60.0%
GGCCAGCTGGAGGTAAAGGT+CGG - tig0017897:69075-69094 None:intergenic 60.0%
TGTGGGCCAGCTGGAGGTAA+AGG - tig0017897:69079-69098 None:intergenic 60.0%
AGTAGAGCATGTGGGCCAGC+TGG - tig0017897:69088-69107 None:intergenic 60.0%
GGCTAATCAAGCCCCTGCGA+AGG + tig0017897:69216-69235 Msa1362650:CDS 60.0%
CAGGTCGTGCATTTGGCAGG+GGG - tig0017897:69006-69025 None:intergenic 60.0%
GGCCAGCTGGAGGTAAAGGT+CGG - tig0017897:69075-69094 None:intergenic 60.0%
TGTGGGCCAGCTGGAGGTAA+AGG - tig0017897:69079-69098 None:intergenic 60.0%
AGTAGAGCATGTGGGCCAGC+TGG - tig0017897:69088-69107 None:intergenic 60.0%
GGCTAATCAAGCCCCTGCGA+AGG + tig0017897:69216-69235 Msa1362650:CDS 60.0%
AGAGCATGTGGGCCAGCTGG+AGG - tig0017897:69085-69104 None:intergenic 65.0%
AGAGCATGTGGGCCAGCTGG+AGG - tig0017897:69085-69104 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
tig0017897 gene 68424 70033 68424 ID=Msa1362650;Name=Msa1362650
tig0017897 mRNA 68424 70033 68424 ID=Msa1362650-mRNA-1;Parent=Msa1362650;Name=Msa1362650-mRNA-1;_AED=0.12;_eAED=0.12;_QI=0|0.66|0.5|0.75|0.66|0.25|4|372|175
tig0017897 exon 68424 68504 68424 ID=Msa1362650-mRNA-1:exon:13132;Parent=Msa1362650-mRNA-1
tig0017897 exon 68998 69237 68998 ID=Msa1362650-mRNA-1:exon:13133;Parent=Msa1362650-mRNA-1
tig0017897 exon 69333 69872 69333 ID=Msa1362650-mRNA-1:exon:13134;Parent=Msa1362650-mRNA-1
tig0017897 exon 69995 70033 69995 ID=Msa1362650-mRNA-1:exon:13135;Parent=Msa1362650-mRNA-1
tig0017897 CDS 68424 68504 68424 ID=Msa1362650-mRNA-1:cds;Parent=Msa1362650-mRNA-1
tig0017897 CDS 68998 69237 68998 ID=Msa1362650-mRNA-1:cds;Parent=Msa1362650-mRNA-1
tig0017897 CDS 69333 69539 69333 ID=Msa1362650-mRNA-1:cds;Parent=Msa1362650-mRNA-1
tig0017897 three_prime_UTR 69540 69872 69540 ID=Msa1362650-mRNA-1:three_prime_utr;Parent=Msa1362650-mRNA-1
tig0017897 three_prime_UTR 69995 70033 69995 ID=Msa1362650-mRNA-1:three_prime_utr;Parent=Msa1362650-mRNA-1
Gene Sequence

>Msa1362650

ATCGCATATATAGGTGTCTTTGGAACCGGGCTATTAGGCGCTTTCTTTTCCAATTTTTTGTTCAAGAAACATCATTTCCAATTTGGCCCCCTGCCAAATGCACGACCTGTTATGCCACTTCCTATTCCATACCAAAGAATCGGAAACAACTCTTTCCGACCTTTACCTCCAGCTGGCCCACATGCTCTACTCCAGATTCTACCTCATCCGAATCTTGGTCCTAATACGTCAAGCCAACAACCAATTGTTGGATATTCTAATTTGATTAGTTCACTCATTGCCCAAGGTGTTATCTCATTGGCTAATCAAGCCCCTGCGAAGGATTTTATTGGCCTTGAGTTTGATCCAAATATTCTCAAGGTTCGTCATGAATATGTAATTTGTGATTTATATGGTGATTTTCCTAGACAATGCACAACTTGTGGCCATCGATTCAAATCCAAAGATGAGCACAGCAGTCACATGGATTGGCATGTGACAAAGAACCGTATGTCTAAAAACTGTAAGCAAAAGTCCTCTCGCAAGTAG
Protein sequence

>Msa1362650

IAYIGVFGTGLLGAFFSNFLFKKHHFQFGPLPNARPVMPLPIPYQRIGNNSFRPLPPAGPHALLQILPHPNLGPNTSSQQPIVGYSNLISSLIAQGVISLANQAPAKDFIGLEFDPNILKVRHEYVICDLYGDFPRQCTTCGHRFKSKDEHSSHMDWHVTKNRMSKNCKQKSSRK*