AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1363030


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1363030 MtrunA17_Chr4g0070271 69.466 131 38 2 1 130 67 196 8.36e-54 167
Msa1363030 MtrunA17_Chr4g0070241 92.188 64 5 0 1 64 1 64 4.94e-37 121
Msa1363030 MtrunA17_Chr2g0301131 37.912 182 53 7 1 130 72 245 1.83e-26 99.4
Msa1363030 MtrunA17_Chr5g0399851 35.659 129 60 3 25 130 11 139 1.12e-18 76.6
Msa1363030 MtrunA17_Chr5g0399881 38.562 153 65 6 7 130 5 157 1.71e-18 76.6
Msa1363030 MtrunA17_Chr5g0399861 35.526 152 70 4 7 130 5 156 1.09e-16 72.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1363030 AT1G19180.2 33.974 156 69 3 2 123 17 172 2.09e-16 72.0
Msa1363030 AT1G19180.1 33.974 156 69 3 2 123 83 238 4.92e-16 72.0
Msa1363030 AT1G19180.3 33.974 156 69 3 2 123 83 238 4.92e-16 72.0
Msa1363030 AT1G74950.1 36.923 130 47 4 30 124 112 241 3.02e-14 67.4

Find 24 sgRNAs with CRISPR-Local

Find 146 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGCTTCACTTCTTCGATTCA+TGG 0.237608 tig0018025:+69722 Msa1363030:CDS
AATGTCAACTGTGCAGTTTC+CGG 0.323565 tig0018025:-69428 None:intergenic
TTTAGCACCTAAAACAAGTA+TGG 0.324800 tig0018025:-70592 None:intergenic
TCGGAATCGCTTCTTTGATA+TGG 0.333476 tig0018025:-70538 None:intergenic
CAACCACCTCCTACACTAAC+TGG 0.374106 tig0018025:+69566 Msa1363030:CDS
TTATTTCTTTAAAGAAGATA+TGG 0.376656 tig0018025:+69686 Msa1363030:intron
TAATGGAGAAGTTATTGTAT+TGG 0.400656 tig0018025:+69457 Msa1363030:CDS
GCTTCTTTGATATGGTGATT+TGG 0.408539 tig0018025:-70530 None:intergenic
TAACTCAATGGTTAACAAAC+CGG 0.425259 tig0018025:+69409 Msa1363030:CDS
GGTGGTTGAAGACTCATATC+AGG 0.439597 tig0018025:-69551 None:intergenic
AGCTCCTCAACTTTGTCAGC+AGG 0.455494 tig0018025:-69488 None:intergenic
TTACAGAACTAATAACTCAA+TGG 0.476118 tig0018025:+69397 Msa1363030:intron
TTCTTCGATTCATGGAGAAA+AGG 0.478429 tig0018025:+69730 Msa1363030:CDS
CAATTACCAGTTAGTGTAGG+AGG 0.522487 tig0018025:-69572 None:intergenic
CTGTGGATTCTTTGTCTCCA+AGG 0.531713 tig0018025:+69253 Msa1363030:CDS
TCATGGAGAAAAGGAAAGCC+AGG 0.536399 tig0018025:+69739 Msa1363030:CDS
CATTTGCAAGCACTCAAACT+CGG 0.560353 tig0018025:+69516 Msa1363030:CDS
AAGTATGGAGTGGATTCAGT+AGG 0.584677 tig0018025:-70577 None:intergenic
CACCTAAAACAAGTATGGAG+TGG 0.594681 tig0018025:-70587 None:intergenic
CTCATATCAGGAATAACTGA+AGG 0.598557 tig0018025:-69539 None:intergenic
GTTTGTGGGAAATAAGTCCA+TGG 0.603000 tig0018025:-69208 None:intergenic
TTACCAGTTAGTGTAGGAGG+TGG 0.608915 tig0018025:-69569 None:intergenic
GAGCAATTACCAGTTAGTGT+AGG 0.610011 tig0018025:-69575 None:intergenic
CTTGGAGACAAAGAATCCAC+AGG 0.648213 tig0018025:-69252 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TAATATTTTGAAACAAATAA+AGG + tig0018025:69610-69629 Msa1363030:intron 10.0%
!!! TAATATTTTGAAACAAATAA+AGG + tig0018025:69610-69629 Msa1363030:intron 10.0%
!!! AAGGAAATATTTTTTGTATT+AGG + tig0018025:69629-69648 Msa1363030:intron 15.0%
!!! AGGAAATATTTTTTGTATTA+GGG + tig0018025:69630-69649 Msa1363030:intron 15.0%
!! AAGAAATAAAATTTATGATC+AGG - tig0018025:69675-69694 None:intergenic 15.0%
!!! TTATTTCTTTAAAGAAGATA+TGG + tig0018025:69686-69705 Msa1363030:intron 15.0%
!!! TAAATTCAAGTTTTTATTCA+AGG + tig0018025:69780-69799 Msa1363030:intron 15.0%
!!! TCAATCAACTAATATTTTTT+TGG + tig0018025:69917-69936 Msa1363030:intron 15.0%
!!! CAATCAACTAATATTTTTTT+GGG + tig0018025:69918-69937 Msa1363030:intron 15.0%
!!! ATTATGAATATTTTGATTAC+CGG - tig0018025:70035-70054 None:intergenic 15.0%
!!! AAGGAAATATTTTTTGTATT+AGG + tig0018025:69629-69648 Msa1363030:intron 15.0%
!!! AGGAAATATTTTTTGTATTA+GGG + tig0018025:69630-69649 Msa1363030:intron 15.0%
!! AAGAAATAAAATTTATGATC+AGG - tig0018025:69675-69694 None:intergenic 15.0%
!!! TTATTTCTTTAAAGAAGATA+TGG + tig0018025:69686-69705 Msa1363030:intron 15.0%
!!! TAAATTCAAGTTTTTATTCA+AGG + tig0018025:69780-69799 Msa1363030:intron 15.0%
!!! TCAATCAACTAATATTTTTT+TGG + tig0018025:69917-69936 Msa1363030:intron 15.0%
!!! CAATCAACTAATATTTTTTT+GGG + tig0018025:69918-69937 Msa1363030:intron 15.0%
!!! ATTATGAATATTTTGATTAC+CGG - tig0018025:70035-70054 None:intergenic 15.0%
!! TAACAGTAACACTATATATT+AGG - tig0018025:70480-70499 None:intergenic 20.0%
!! TAACAGTAACACTATATATT+AGG - tig0018025:70480-70499 None:intergenic 20.0%
! TTACAGAACTAATAACTCAA+TGG + tig0018025:69397-69416 Msa1363030:intron 25.0%
!!! CAGTTGACATTGTTTTATAA+TGG + tig0018025:69440-69459 Msa1363030:CDS 25.0%
! TAATGGAGAAGTTATTGTAT+TGG + tig0018025:69457-69476 Msa1363030:CDS 25.0%
!!! TTGAATTTAATTTGTCTGAG+AGG - tig0018025:69769-69788 None:intergenic 25.0%
! TTGTTTCAACATTTCAATCA+AGG - tig0018025:69895-69914 None:intergenic 25.0%
!!! GTAAAATGTGATTTTTGAGA+TGG - tig0018025:70268-70287 None:intergenic 25.0%
! GTAACACTATATATTAGGTT+AGG - tig0018025:70475-70494 None:intergenic 25.0%
! TTACAGAACTAATAACTCAA+TGG + tig0018025:69397-69416 Msa1363030:intron 25.0%
!!! CAGTTGACATTGTTTTATAA+TGG + tig0018025:69440-69459 Msa1363030:CDS 25.0%
! TAATGGAGAAGTTATTGTAT+TGG + tig0018025:69457-69476 Msa1363030:CDS 25.0%
!!! TTGAATTTAATTTGTCTGAG+AGG - tig0018025:69769-69788 None:intergenic 25.0%
! TTGTTTCAACATTTCAATCA+AGG - tig0018025:69895-69914 None:intergenic 25.0%
!!! GTAAAATGTGATTTTTGAGA+TGG - tig0018025:70268-70287 None:intergenic 25.0%
! GTAACACTATATATTAGGTT+AGG - tig0018025:70475-70494 None:intergenic 25.0%
TAACTCAATGGTTAACAAAC+CGG + tig0018025:69409-69428 Msa1363030:CDS 30.0%
GTTGTCGTAACTTTGTAATT+TGG + tig0018025:70162-70181 Msa1363030:intron 30.0%
!! AGTAGTTGATGATATGCATA+AGG - tig0018025:70235-70254 None:intergenic 30.0%
!! TCAATTTCTAGTAACACAAG+TGG - tig0018025:70337-70356 None:intergenic 30.0%
TAACTCAATGGTTAACAAAC+CGG + tig0018025:69409-69428 Msa1363030:CDS 30.0%
GTTGTCGTAACTTTGTAATT+TGG + tig0018025:70162-70181 Msa1363030:intron 30.0%
!! AGTAGTTGATGATATGCATA+AGG - tig0018025:70235-70254 None:intergenic 30.0%
!! TCAATTTCTAGTAACACAAG+TGG - tig0018025:70337-70356 None:intergenic 30.0%
!!! TTTTTTGGTGTCACGTTTGT+GGG - tig0018025:69225-69244 None:intergenic 35.0%
! AGAAGCACAAAAACATACCT+TGG - tig0018025:69273-69292 None:intergenic 35.0%
!!! TTTTTTGCACACAAGCAATG+GGG - tig0018025:69334-69353 None:intergenic 35.0%
!!! CTTTTTTGCACACAAGCAAT+GGG - tig0018025:69335-69354 None:intergenic 35.0%
!!! ACTTTTTTGCACACAAGCAA+TGG - tig0018025:69336-69355 None:intergenic 35.0%
CTCATATCAGGAATAACTGA+AGG - tig0018025:69542-69561 None:intergenic 35.0%
TTCTTCGATTCATGGAGAAA+AGG + tig0018025:69730-69749 Msa1363030:CDS 35.0%
!!! CAACTTTCGTGTTGTTTTCA+CGG - tig0018025:70146-70165 None:intergenic 35.0%
TAGAATTGGATGGAATCAGT+TGG - tig0018025:70294-70313 None:intergenic 35.0%
! AACTGATTCCATCCAATTCT+AGG + tig0018025:70293-70312 Msa1363030:intron 35.0%
ACTGCAAAGTAAGTAACAAG+AGG - tig0018025:70429-70448 None:intergenic 35.0%
ATTTCCTGAGTGATGTTGTT+TGG + tig0018025:70449-70468 Msa1363030:intron 35.0%
GCTTCTTTGATATGGTGATT+TGG - tig0018025:70533-70552 None:intergenic 35.0%
!!! GTGGATTCAGTAGGTTTTTT+TGG - tig0018025:70571-70590 None:intergenic 35.0%
!!! TTTTTTGGTGTCACGTTTGT+GGG - tig0018025:69225-69244 None:intergenic 35.0%
! AGAAGCACAAAAACATACCT+TGG - tig0018025:69273-69292 None:intergenic 35.0%
!!! TTTTTTGCACACAAGCAATG+GGG - tig0018025:69334-69353 None:intergenic 35.0%
!!! CTTTTTTGCACACAAGCAAT+GGG - tig0018025:69335-69354 None:intergenic 35.0%
!!! ACTTTTTTGCACACAAGCAA+TGG - tig0018025:69336-69355 None:intergenic 35.0%
CTCATATCAGGAATAACTGA+AGG - tig0018025:69542-69561 None:intergenic 35.0%
TTCTTCGATTCATGGAGAAA+AGG + tig0018025:69730-69749 Msa1363030:CDS 35.0%
!!! CAACTTTCGTGTTGTTTTCA+CGG - tig0018025:70146-70165 None:intergenic 35.0%
TAGAATTGGATGGAATCAGT+TGG - tig0018025:70294-70313 None:intergenic 35.0%
! AACTGATTCCATCCAATTCT+AGG + tig0018025:70293-70312 Msa1363030:intron 35.0%
ACTGCAAAGTAAGTAACAAG+AGG - tig0018025:70429-70448 None:intergenic 35.0%
ATTTCCTGAGTGATGTTGTT+TGG + tig0018025:70449-70468 Msa1363030:intron 35.0%
GCTTCTTTGATATGGTGATT+TGG - tig0018025:70533-70552 None:intergenic 35.0%
!!! GTGGATTCAGTAGGTTTTTT+TGG - tig0018025:70571-70590 None:intergenic 35.0%
!!! GTTTTTTGGTGTCACGTTTG+TGG - tig0018025:69226-69245 None:intergenic 40.0%
ACCAAAAAACGTTACACCTG+TGG + tig0018025:69236-69255 Msa1363030:CDS 40.0%
!!! TCCACAGGTGTAACGTTTTT+TGG - tig0018025:69240-69259 None:intergenic 40.0%
GCAATGGGGCAAAACAAAAA+TGG - tig0018025:69320-69339 None:intergenic 40.0%
AATGTCAACTGTGCAGTTTC+CGG - tig0018025:69431-69450 None:intergenic 40.0%
! TTTTCCTGCTGACAAAGTTG+AGG + tig0018025:69484-69503 Msa1363030:CDS 40.0%
CATTTGCAAGCACTCAAACT+CGG + tig0018025:69516-69535 Msa1363030:CDS 40.0%
CAATTACCAGTTAGTGTAGG+AGG - tig0018025:69575-69594 None:intergenic 40.0%
GAGCAATTACCAGTTAGTGT+AGG - tig0018025:69578-69597 None:intergenic 40.0%
AGCTTCACTTCTTCGATTCA+TGG + tig0018025:69722-69741 Msa1363030:CDS 40.0%
ATAGACAGAGACATGGAGAA+GGG - tig0018025:69872-69891 None:intergenic 40.0%
ATCAAGGATAGACAGAGACA+TGG - tig0018025:69879-69898 None:intergenic 40.0%
CAAGCAAAATCAAGGACTTC+CGG + tig0018025:70013-70032 Msa1363030:intron 40.0%
CACTATTTATGTTCCTCTCG+TGG - tig0018025:70081-70100 None:intergenic 40.0%
TTGTGCTCATGCTCTTACAA+GGG - tig0018025:70107-70126 None:intergenic 40.0%
!!! TTCGTGTTGTTTTCACGGTA+CGG - tig0018025:70141-70160 None:intergenic 40.0%
TCTAGTAACACAAGTGGCTT+TGG - tig0018025:70331-70350 None:intergenic 40.0%
CTCTAGTCCATTGTGCAAAT+TGG + tig0018025:70399-70418 Msa1363030:intron 40.0%
AGGCAAACCAATTTGCACAA+TGG - tig0018025:70409-70428 None:intergenic 40.0%
TCGGAATCGCTTCTTTGATA+TGG - tig0018025:70541-70560 None:intergenic 40.0%
!!! AGGTTTTTTTGGAGCAGAGT+CGG - tig0018025:70560-70579 None:intergenic 40.0%
! AAGTATGGAGTGGATTCAGT+AGG - tig0018025:70580-70599 None:intergenic 40.0%
!!! GTTTTTTGGTGTCACGTTTG+TGG - tig0018025:69226-69245 None:intergenic 40.0%
ACCAAAAAACGTTACACCTG+TGG + tig0018025:69236-69255 Msa1363030:CDS 40.0%
!!! TCCACAGGTGTAACGTTTTT+TGG - tig0018025:69240-69259 None:intergenic 40.0%
GCAATGGGGCAAAACAAAAA+TGG - tig0018025:69320-69339 None:intergenic 40.0%
AATGTCAACTGTGCAGTTTC+CGG - tig0018025:69431-69450 None:intergenic 40.0%
! TTTTCCTGCTGACAAAGTTG+AGG + tig0018025:69484-69503 Msa1363030:CDS 40.0%
CATTTGCAAGCACTCAAACT+CGG + tig0018025:69516-69535 Msa1363030:CDS 40.0%
CAATTACCAGTTAGTGTAGG+AGG - tig0018025:69575-69594 None:intergenic 40.0%
GAGCAATTACCAGTTAGTGT+AGG - tig0018025:69578-69597 None:intergenic 40.0%
AGCTTCACTTCTTCGATTCA+TGG + tig0018025:69722-69741 Msa1363030:CDS 40.0%
ATAGACAGAGACATGGAGAA+GGG - tig0018025:69872-69891 None:intergenic 40.0%
ATCAAGGATAGACAGAGACA+TGG - tig0018025:69879-69898 None:intergenic 40.0%
CAAGCAAAATCAAGGACTTC+CGG + tig0018025:70013-70032 Msa1363030:intron 40.0%
CACTATTTATGTTCCTCTCG+TGG - tig0018025:70081-70100 None:intergenic 40.0%
TTGTGCTCATGCTCTTACAA+GGG - tig0018025:70107-70126 None:intergenic 40.0%
!!! TTCGTGTTGTTTTCACGGTA+CGG - tig0018025:70141-70160 None:intergenic 40.0%
TCTAGTAACACAAGTGGCTT+TGG - tig0018025:70331-70350 None:intergenic 40.0%
CTCTAGTCCATTGTGCAAAT+TGG + tig0018025:70399-70418 Msa1363030:intron 40.0%
AGGCAAACCAATTTGCACAA+TGG - tig0018025:70409-70428 None:intergenic 40.0%
TCGGAATCGCTTCTTTGATA+TGG - tig0018025:70541-70560 None:intergenic 40.0%
!!! AGGTTTTTTTGGAGCAGAGT+CGG - tig0018025:70560-70579 None:intergenic 40.0%
! AAGTATGGAGTGGATTCAGT+AGG - tig0018025:70580-70599 None:intergenic 40.0%
CTTGGAGACAAAGAATCCAC+AGG - tig0018025:69255-69274 None:intergenic 45.0%
CTGTGGATTCTTTGTCTCCA+AGG + tig0018025:69253-69272 Msa1363030:CDS 45.0%
GGTGGTTGAAGACTCATATC+AGG - tig0018025:69554-69573 None:intergenic 45.0%
TTACCAGTTAGTGTAGGAGG+TGG - tig0018025:69572-69591 None:intergenic 45.0%
TCATGGAGAAAAGGAAAGCC+AGG + tig0018025:69739-69758 Msa1363030:CDS 45.0%
! ATTTGTCTGAGAGGCATACC+TGG - tig0018025:69760-69779 None:intergenic 45.0%
GATAGACAGAGACATGGAGA+AGG - tig0018025:69873-69892 None:intergenic 45.0%
! CTTGATTTTGCTTGCGTCGA+AGG - tig0018025:70007-70026 None:intergenic 45.0%
CTTCGACGCAAGCAAAATCA+AGG + tig0018025:70005-70024 Msa1363030:intron 45.0%
TCATAGTCAAGAACCACGAG+AGG + tig0018025:70065-70084 Msa1363030:intron 45.0%
GTTGTGCTCATGCTCTTACA+AGG - tig0018025:70108-70127 None:intergenic 45.0%
TCAGCAAGCCTAGAATTGGA+TGG - tig0018025:70304-70323 None:intergenic 45.0%
CTCTTCAGCAAGCCTAGAAT+TGG - tig0018025:70308-70327 None:intergenic 45.0%
TAGGCCAAACAACATCACTC+AGG - tig0018025:70456-70475 None:intergenic 45.0%
CTTGGAGACAAAGAATCCAC+AGG - tig0018025:69255-69274 None:intergenic 45.0%
CTGTGGATTCTTTGTCTCCA+AGG + tig0018025:69253-69272 Msa1363030:CDS 45.0%
GGTGGTTGAAGACTCATATC+AGG - tig0018025:69554-69573 None:intergenic 45.0%
TTACCAGTTAGTGTAGGAGG+TGG - tig0018025:69572-69591 None:intergenic 45.0%
TCATGGAGAAAAGGAAAGCC+AGG + tig0018025:69739-69758 Msa1363030:CDS 45.0%
! ATTTGTCTGAGAGGCATACC+TGG - tig0018025:69760-69779 None:intergenic 45.0%
GATAGACAGAGACATGGAGA+AGG - tig0018025:69873-69892 None:intergenic 45.0%
! CTTGATTTTGCTTGCGTCGA+AGG - tig0018025:70007-70026 None:intergenic 45.0%
CTTCGACGCAAGCAAAATCA+AGG + tig0018025:70005-70024 Msa1363030:intron 45.0%
TCATAGTCAAGAACCACGAG+AGG + tig0018025:70065-70084 Msa1363030:intron 45.0%
GTTGTGCTCATGCTCTTACA+AGG - tig0018025:70108-70127 None:intergenic 45.0%
TCAGCAAGCCTAGAATTGGA+TGG - tig0018025:70304-70323 None:intergenic 45.0%
CTCTTCAGCAAGCCTAGAAT+TGG - tig0018025:70308-70327 None:intergenic 45.0%
TAGGCCAAACAACATCACTC+AGG - tig0018025:70456-70475 None:intergenic 45.0%
AGCTCCTCAACTTTGTCAGC+AGG - tig0018025:69491-69510 None:intergenic 50.0%
CAACCACCTCCTACACTAAC+TGG + tig0018025:69566-69585 Msa1363030:CDS 50.0%
AGCTCCTCAACTTTGTCAGC+AGG - tig0018025:69491-69510 None:intergenic 50.0%
CAACCACCTCCTACACTAAC+TGG + tig0018025:69566-69585 Msa1363030:CDS 50.0%
Chromosome Type Strat End Strand Name
tig0018025 gene 69210 70606 69210 ID=Msa1363030;Name=Msa1363030
tig0018025 mRNA 69210 70606 69210 ID=Msa1363030-mRNA-1;Parent=Msa1363030;Name=Msa1363030-mRNA-1;_AED=0.33;_eAED=0.34;_QI=0|0|0|0.75|1|1|4|0|131
tig0018025 exon 69210 69274 69210 ID=Msa1363030-mRNA-1:exon:13342;Parent=Msa1363030-mRNA-1
tig0018025 exon 69403 69587 69403 ID=Msa1363030-mRNA-1:exon:13343;Parent=Msa1363030-mRNA-1
tig0018025 exon 69700 69760 69700 ID=Msa1363030-mRNA-1:exon:13344;Parent=Msa1363030-mRNA-1
tig0018025 exon 70522 70606 70522 ID=Msa1363030-mRNA-1:exon:13345;Parent=Msa1363030-mRNA-1
tig0018025 CDS 69210 69274 69210 ID=Msa1363030-mRNA-1:cds;Parent=Msa1363030-mRNA-1
tig0018025 CDS 69403 69587 69403 ID=Msa1363030-mRNA-1:cds;Parent=Msa1363030-mRNA-1
tig0018025 CDS 69700 69760 69700 ID=Msa1363030-mRNA-1:cds;Parent=Msa1363030-mRNA-1
tig0018025 CDS 70522 70606 70522 ID=Msa1363030-mRNA-1:cds;Parent=Msa1363030-mRNA-1
Gene Sequence

>Msa1363030

ATGGACTTATTTCCCACAAACGTGACACCAAAAAACGTTACACCTGTGGATTCTTTGTCTCCAAGAACTAATAACTCAATGGTTAACAAACCGGAAACTGCACAGTTGACATTGTTTTATAATGGAGAAGTTATTGTATTGGATGATTTTCCTGCTGACAAAGTTGAGGAGCTTAAGTCATTTGCAAGCACTCAAACTCGGCCTTCAGTTATTCCTGATATGAGTCTTCAACCACCTCCTACACTAACTGAAGATATGGTAATTGCTAGAAAAGCTTCACTTCTTCGATTCATGGAGAAAAGGAAAGCCAGAGTTTCTGCCAAATCACCATATCAAAGAAGCGATTCCGACTCTGCTCCAAAAAAACCTACTGAATCCACTCCATACTTGTTTTAG

Protein sequence

>Msa1363030

MDLFPTNVTPKNVTPVDSLSPRTNNSMVNKPETAQLTLFYNGEVIVLDDFPADKVEELKSFASTQTRPSVIPDMSLQPPPTLTEDMVIARKASLLRFMEKRKARVSAKSPYQRSDSDSAPKKPTESTPYLF*