AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1364310

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1364310 MtrunA17_Chr1g0208771 95.778 450 19 0 35 484 12 461 0.0 910
Msa1364310 MtrunA17_Chr8g0390841 66.733 505 136 7 1 482 24 519 0.0 676
Msa1364310 MtrunA17_Chr1g0163071 50.831 301 132 7 36 324 2 298 8.84e-91 279
Msa1364310 MtrunA17_Chr7g0233561 35.556 315 166 10 18 313 16 312 1.84e-53 182
Msa1364310 MtrunA17_Chr3g0131891 36.218 312 164 9 15 313 14 303 2.59e-53 182
Msa1364310 MtrunA17_Chr7g0233541 36.149 296 154 11 36 313 2 280 5.16e-49 170
Msa1364310 MtrunA17_Chr7g0233531 34.000 300 138 9 36 313 2 263 3.71e-43 154
Msa1364310 MtrunA17_Chr1g0163181 27.754 472 263 15 29 479 14 428 5.40e-43 157
Msa1364310 MtrunA17_Chr3g0103181 32.258 310 155 11 18 312 10 279 2.51e-42 151
Msa1364310 MtrunA17_Chr3g0103181 28.636 220 131 7 279 480 68 279 7.11e-12 65.9
Msa1364310 MtrunA17_Chr7g0233521 32.203 295 150 8 36 313 2 263 2.24e-41 149
Msa1364310 MtrunA17_Chr3g0103171 31.058 293 155 8 35 312 5 265 8.61e-39 142
Msa1364310 MtrunA17_Chr3g0103171 30.000 270 150 11 240 480 6 265 1.31e-16 79.7
Msa1364310 MtrunA17_Chr3g0103191 31.769 277 139 8 18 279 10 251 1.59e-38 140
Msa1364310 MtrunA17_Chr7g0233571 34.529 223 123 5 20 235 10 216 3.57e-35 130
Msa1364310 MtrunA17_Chr3g0103151 40.667 150 80 2 18 160 10 157 3.56e-33 123
Msa1364310 MtrunA17_Chr4g0067431 27.414 321 201 13 15 317 10 316 8.88e-32 124
Msa1364310 MtrunA17_Chr1g0163221 26.646 319 205 9 10 320 1 298 7.32e-31 122
Msa1364310 MtrunA17_Chr1g0163201 31.000 300 162 10 35 317 18 289 1.58e-29 119
Msa1364310 MtrunA17_Chr3g0103201 31.890 254 123 7 75 312 1 220 8.20e-29 113
Msa1364310 MtrunA17_Chr1g0163261 26.480 321 193 8 19 327 8 297 1.05e-27 112
Msa1364310 MtrunA17_Chr1g0163251 29.204 339 193 15 29 357 14 315 1.09e-26 111
Msa1364310 MtrunA17_Chr5g0429931 26.460 291 146 9 35 314 10 243 1.02e-24 102
Msa1364310 MtrunA17_Chr5g0419201 32.558 172 92 5 5 160 2 165 1.55e-24 100
Msa1364310 MtrunA17_Chr1g0163231 26.316 304 177 10 20 311 9 277 5.49e-23 99.0
Msa1364310 MtrunA17_Chr1g0154071 25.723 346 205 12 21 322 157 494 5.42e-21 95.9
Msa1364310 MtrunA17_Chr1g0163131 27.302 315 185 11 15 309 13 303 5.90e-21 93.6
Msa1364310 MtrunA17_Chr1g0154161 25.902 305 185 11 25 317 36 311 5.95e-21 93.6
Msa1364310 MtrunA17_Chr1g0171611 25.974 308 161 9 20 311 9 265 8.28e-21 92.8
Msa1364310 MtrunA17_Chr3g0077411 24.204 314 168 8 17 314 259 518 2.85e-20 93.6
Msa1364310 MtrunA17_Chr3g0077411 22.027 513 327 17 20 480 25 516 6.43e-20 92.8
Msa1364310 MtrunA17_Chr1g0163111 27.586 290 133 11 212 484 2 231 5.24e-20 89.0
Msa1364310 MtrunA17_Chr4g0033961 37.963 108 67 0 20 127 6 113 1.69e-19 90.9
Msa1364310 MtrunA17_Chr4g0033961 38.931 131 72 4 201 324 270 399 4.31e-18 86.3
Msa1364310 MtrunA17_Chr1g0154111 25.836 329 195 11 18 301 24 348 8.63e-19 89.4
Msa1364310 MtrunA17_Chr1g0154191 25.074 339 200 13 25 317 28 358 1.23e-18 87.4
Msa1364310 MtrunA17_Chr6g0467351 29.801 151 101 2 16 165 11 157 2.53e-18 84.0
Msa1364310 MtrunA17_Chr1g0154131 25.887 282 176 8 34 301 196 458 1.15e-17 85.5
Msa1364310 MtrunA17_Chr1g0162441 35.036 137 74 3 20 141 7 143 3.05e-17 84.0
Msa1364310 MtrunA17_Chr1g0162441 36.434 129 72 5 201 321 274 400 3.69e-15 77.4
Msa1364310 MtrunA17_Chr1g0154151 22.397 317 192 10 36 317 20 317 5.54e-16 79.0
Msa1364310 MtrunA17_Chr6g0476181 31.818 154 97 4 36 189 2 147 1.50e-15 74.3
Msa1364310 MtrunA17_Chr3g0077461 25.685 292 141 9 20 304 237 459 1.72e-15 78.6
Msa1364310 MtrunA17_Chr3g0077461 33.333 84 56 0 35 118 22 105 1.83e-12 69.3
Msa1364310 MtrunA17_Chr3g0077451 26.829 164 104 3 34 181 10 173 1.84e-15 75.9
Msa1364310 MtrunA17_Chr1g0163191 37.778 90 54 1 29 118 14 101 2.52e-15 73.6
Msa1364310 MtrunA17_Chr3g0103161 37.288 118 69 3 368 484 14 127 3.98e-15 72.0
Msa1364310 MtrunA17_Chr3g0103161 31.818 110 61 5 215 315 22 126 4.66e-11 60.5
Msa1364310 MtrunA17_Chr1g0163101 33.613 119 76 2 14 132 11 126 3.07e-14 70.9
Msa1364310 MtrunA17_Chr1g0163241 38.095 84 50 1 35 118 22 103 4.81e-14 70.1
Msa1364310 MtrunA17_Chr1g0163121 40.426 94 53 2 34 127 9 99 4.97e-14 69.3
Msa1364310 MtrunA17_Chr1g0163061 33.010 103 68 1 17 118 12 114 3.04e-13 70.1
Msa1364310 MtrunA17_Chr1g0163081 42.169 83 45 2 36 118 6 85 7.18e-13 66.2
Msa1364310 MtrunA17_Chr1g0163151 33.628 113 64 4 212 313 2 114 1.05e-12 67.4
Msa1364310 MtrunA17_Chr4g0009491 29.677 155 98 4 12 161 213 361 3.83e-12 68.2
Msa1364310 MtrunA17_Chr1g0162411 35.354 99 62 1 20 118 7 103 4.61e-12 67.8
Msa1364310 MtrunA17_Chr3g0077381 31.765 85 58 0 34 118 34 118 2.34e-11 65.9
Msa1364310 MtrunA17_Chr3g0077381 29.221 154 100 3 12 161 235 383 2.49e-11 65.5
Msa1364310 MtrunA17_Chr3g0077361 31.000 100 68 1 20 118 40 139 3.52e-11 65.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1364310 AT3G18990.1 32.344 337 185 11 20 316 6 339 1.50e-42 154
Msa1364310 AT3G18990.2 32.047 337 186 11 20 316 6 339 1.03e-41 153
Msa1364310 AT1G49480.3 31.977 172 88 7 171 316 56 224 6.59e-17 80.1
Msa1364310 AT1G49480.3 35.897 117 66 4 370 481 109 221 5.37e-11 63.2
Msa1364310 AT1G49480.1 31.977 172 88 7 171 316 56 224 6.59e-17 80.1
Msa1364310 AT1G49480.1 35.897 117 66 4 370 481 109 221 5.37e-11 63.2
Msa1364310 AT1G49480.2 31.977 172 88 7 171 316 56 224 6.59e-17 80.1
Msa1364310 AT1G49480.2 35.897 117 66 4 370 481 109 221 5.37e-11 63.2
Msa1364310 AT4G01580.1 36.538 104 66 0 19 122 29 132 9.35e-17 79.0
Msa1364310 AT3G18960.2 34.615 104 68 0 19 122 29 132 5.97e-16 76.6
Msa1364310 AT3G18960.1 34.615 104 68 0 19 122 29 132 9.23e-16 76.6
Msa1364310 AT4G33280.1 26.333 300 198 10 34 320 37 326 1.13e-15 78.6
Msa1364310 AT1G49475.1 31.667 120 77 2 19 138 33 147 2.04e-14 72.0
Msa1364310 AT3G18960.3 35.227 88 57 0 35 122 8 95 1.77e-11 63.2

Find 77 sgRNAs with CRISPR-Local

Find 135 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GAACAATCCAATTGAAATTT+TGG 0.104888 tig0018115:-54439 Msa1364310:CDS
CCTAAACTTGTCCAAGTTAA+AGG 0.118978 tig0018115:-54294 Msa1364310:intron
ATCTGATAATAATTTGAAAT+TGG 0.143719 tig0018115:-53745 Msa1364310:CDS
CTCAAATTGATAGTGAAATT+TGG 0.167485 tig0018115:+53071 None:intergenic
GAACTTGAAATTTGTGATTT+TGG 0.194976 tig0018115:+54387 None:intergenic
GTGAGACTGTACATATTCTT+AGG 0.199034 tig0018115:+55134 None:intergenic
GGAAATACATTTGACTAAAA+AGG 0.231817 tig0018115:-54637 Msa1364310:CDS
CTTGACCATGCTTCACATTC+AGG 0.239372 tig0018115:+54141 None:intergenic
TATCATGACAGCAGTGAAAA+AGG 0.253877 tig0018115:+54049 None:intergenic
TTCTATTGATCATCTCAAAA+AGG 0.267399 tig0018115:+53712 None:intergenic
CCTTCACCCAACTTTGTTGC+TGG 0.270614 tig0018115:+52750 None:intergenic
CCTTTAACTTGGACAAGTTT+AGG 0.279816 tig0018115:+54294 None:intergenic
ACCCAACTTTGTTGCTGGTT+CGG 0.316172 tig0018115:+52755 None:intergenic
CGAAATCGCCCTTCAGGTCT+TGG 0.340066 tig0018115:-53835 Msa1364310:CDS
TCAATTTGAGATCAAGTCAT+TGG 0.345291 tig0018115:-53058 Msa1364310:CDS
TGCTCATCCAAAATTTCAAT+TGG 0.347442 tig0018115:+54432 None:intergenic
TATTTCTCAGTGAAAGCATT+TGG 0.349857 tig0018115:+54714 None:intergenic
TCTGATAATAATTTGAAATT+GGG 0.355411 tig0018115:-53744 Msa1364310:CDS
GTACATATTCTTAGGGTTGC+TGG 0.358212 tig0018115:+55142 None:intergenic
GACTGCAATGTTACAGTTTG+AGG 0.362858 tig0018115:-52814 Msa1364310:CDS
TTCATTCTCGAGCAAGTACT+TGG 0.373540 tig0018115:-52850 Msa1364310:CDS
AAAACTATTATGTCGTATAA+AGG 0.388718 tig0018115:-53480 Msa1364310:intron
GCAAAGCGAAATCGCCCTTC+AGG 0.399129 tig0018115:-53841 Msa1364310:CDS
GAAATTGAATATCGTGTTGA+CGG 0.403003 tig0018115:-54480 Msa1364310:CDS
TGTAGAAGAAAATAAATTGC+AGG 0.404615 tig0018115:-52715 Msa1364310:CDS
AAGCACAACATCTTCCTTGT+TGG 0.409928 tig0018115:+52665 None:intergenic
CAAAGTGGATGTGAATTGAT+TGG 0.417163 tig0018115:+53100 None:intergenic
CTTCACATTCAGGCATTTAG+TGG 0.427188 tig0018115:+54151 None:intergenic
ATATTTGGATTCAAAGGGGT+TGG 0.428451 tig0018115:-54608 Msa1364310:CDS
ATTTCCCATTCTTTCTCATC+GGG 0.433534 tig0018115:+54654 None:intergenic
ATGGTGATATTTGGATTCAA+AGG 0.444319 tig0018115:-54614 Msa1364310:CDS
AATTTATTATACCTTTAACT+TGG 0.446187 tig0018115:+54283 None:intergenic
AACTTTGTTGCTGGTTCGGA+TGG 0.454779 tig0018115:+52759 None:intergenic
TCCGAACCAGCAACAAAGTT+GGG 0.458098 tig0018115:-52756 Msa1364310:CDS
TGGTGATATTTGGATTCAAA+GGG 0.458234 tig0018115:-54613 Msa1364310:CDS
GGTCTACTCTTCTTGTGAGA+AGG 0.460496 tig0018115:+54408 None:intergenic
ATTCTTAGGGTTGCTGGTCA+TGG 0.460769 tig0018115:+55148 None:intergenic
TTGGATTCAAAGGGGTTGGA+AGG 0.464926 tig0018115:-54604 Msa1364310:CDS
AGACTCCCGATGAGAAAGAA+TGG 0.468520 tig0018115:-54659 Msa1364310:CDS
CAATCAATTCACATCCACTT+TGG 0.477065 tig0018115:-53099 Msa1364310:CDS
TCTTGGATGATGAGAGAACT+TGG 0.477954 tig0018115:-53818 Msa1364310:CDS
TTTGAATCAGTTCATGGAGA+TGG 0.482390 tig0018115:-53546 Msa1364310:intron
ACACATTATTCATTAGATCA+TGG 0.484576 tig0018115:-54573 Msa1364310:CDS
TTCATGGAGATGGAATTAAC+TGG 0.486836 tig0018115:-53536 Msa1364310:CDS
TATTTCCCATTCTTTCTCAT+CGG 0.492052 tig0018115:+54653 None:intergenic
CAATTTGAGATCAAGTCATT+GGG 0.498692 tig0018115:-53057 Msa1364310:CDS
TGAGACTGTACATATTCTTA+GGG 0.500244 tig0018115:+55135 None:intergenic
GACTCCCGATGAGAAAGAAT+GGG 0.507920 tig0018115:-54658 Msa1364310:CDS
TTGGATTACCTTGTGGTGGA+AGG 0.508508 tig0018115:+53637 None:intergenic
ATCCGAACCAGCAACAAAGT+TGG 0.508654 tig0018115:-52757 Msa1364310:CDS
GGATAATACATCACTTGTAC+AGG 0.514406 tig0018115:+52780 None:intergenic
ACCTCAACAAATATTCAAGA+TGG 0.526205 tig0018115:-55080 Msa1364310:CDS
AATTAGTACTTTAAAAGCAT+AGG 0.530987 tig0018115:+53686 None:intergenic
GCTTTCACTGAGAAATATAG+TGG 0.532968 tig0018115:-54708 Msa1364310:CDS
TCTCATCATCCAAGACCTGA+AGG 0.534348 tig0018115:+53826 None:intergenic
CAACAAAGTTGGGTGAAGGT+TGG 0.542982 tig0018115:-52746 Msa1364310:CDS
TGGATTACCTTGTGGTGGAA+GGG 0.545050 tig0018115:+53638 None:intergenic
GAAGAAAATAAATTGCAGGC+TGG 0.548366 tig0018115:-52711 Msa1364310:CDS
TCGGGAGTCTTGAGAAACAT+TGG 0.550403 tig0018115:+54672 None:intergenic
AACTCAAAGAAATTCACTGC+AGG 0.555404 tig0018115:-53165 Msa1364310:CDS
ATTAACTGGTTAGAAACTGC+AGG 0.556059 tig0018115:-53522 Msa1364310:CDS
AAAGAAATTCACTGCAGGCT+AGG 0.559813 tig0018115:-53160 Msa1364310:CDS
TCCATCTTGAATATTTGTTG+AGG 0.562475 tig0018115:+55079 None:intergenic
TTCATTAGATCATGGTCACA+TGG 0.571925 tig0018115:-54565 Msa1364310:CDS
CATCTTGGATTACCTTGTGG+TGG 0.585751 tig0018115:+53633 None:intergenic
GGTGATATTTGGATTCAAAG+GGG 0.604343 tig0018115:-54612 Msa1364310:CDS
TTCATATGTTAATCAATACA+TGG 0.607228 tig0018115:-54024 Msa1364310:intron
CTCATCATCCAAGACCTGAA+GGG 0.610171 tig0018115:+53827 None:intergenic
ATTGACACAAGTGAAGATGT+CGG 0.614793 tig0018115:-54342 Msa1364310:CDS
TATGTAAACCTCAAAATGAG+AGG 0.618304 tig0018115:+54521 None:intergenic
CCAGCAACAAAGTTGGGTGA+AGG 0.622086 tig0018115:-52750 Msa1364310:CDS
ACGGTAATAATCAACATGAA+CGG 0.624679 tig0018115:-54461 Msa1364310:CDS
TCATTCTCGAGCAAGTACTT+GGG 0.630141 tig0018115:-52849 Msa1364310:CDS
CAACATTCCCTTCCACCACA+AGG 0.632695 tig0018115:-53645 Msa1364310:intron
TGAAGCATGGTCAAGAGCAG+AGG 0.641697 tig0018115:-54133 Msa1364310:CDS
TTGGCATACCTCAGGAACGA+AGG 0.641759 tig0018115:-53189 Msa1364310:intron
AAATGCCTGAATGTGAAGCA+TGG 0.702749 tig0018115:-54146 Msa1364310:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATTTATTATACCTTTAACT+TGG + tig0018115:53507-53526 None:intergenic 15.0%
!!! ACTTTTGTTCAAAATTTAAA+GGG + tig0018115:53913-53932 None:intergenic 15.0%
!!! TACTTTTGTTCAAAATTTAA+AGG + tig0018115:53914-53933 None:intergenic 15.0%
!!! ATCTGATAATAATTTGAAAT+TGG - tig0018115:54042-54061 Msa1364310:CDS 15.0%
!!! TCTGATAATAATTTGAAATT+GGG - tig0018115:54043-54062 Msa1364310:CDS 15.0%
!! AAAGATTACTAATAACTTTA+AGG + tig0018115:54187-54206 None:intergenic 15.0%
!!! TAAAGTTATTAGTAATCTTT+TGG - tig0018115:54187-54206 Msa1364310:intron 15.0%
!!! TAATACAAAATGTTGTTTAA+TGG - tig0018115:54439-54458 Msa1364310:CDS 15.0%
!! TTAAAAAATGGAAAGAAAGA+AGG + tig0018115:52753-52772 None:intergenic 20.0%
!! ATCATACAGTACTTAAATTT+CGG + tig0018115:52787-52806 None:intergenic 20.0%
!!! TTTTCTTAGAAAGATACATA+TGG + tig0018115:52901-52920 None:intergenic 20.0%
!!! TCAATTCTCAATTTTTTAAG+TGG + tig0018115:53430-53449 None:intergenic 20.0%
!! AGGTATAATAAATTCATGAT+TGG - tig0018115:53513-53532 Msa1364310:CDS 20.0%
!! TTCATATGTTAATCAATACA+TGG - tig0018115:53763-53782 Msa1364310:CDS 20.0%
!!! TTTTGAACAAAAGTATTTGA+AGG - tig0018115:53919-53938 Msa1364310:intron 20.0%
!!! AATTAGTACTTTAAAAGCAT+AGG + tig0018115:54104-54123 None:intergenic 20.0%
!! AAAACTATTATGTCGTATAA+AGG - tig0018115:54307-54326 Msa1364310:CDS 20.0%
!!! AATTGATTATATGCTTTTGT+TGG - tig0018115:54579-54598 Msa1364310:CDS 20.0%
!! AATCACTAGATATATGTATT+TGG + tig0018115:54799-54818 None:intergenic 20.0%
! GTCGTTCAAAAGTTAAAAAA+TGG + tig0018115:52765-52784 None:intergenic 25.0%
! ATTAAAAGTTCAAGTGAGTT+AGG + tig0018115:52859-52878 None:intergenic 25.0%
! GGAAATACATTTGACTAAAA+AGG - tig0018115:53150-53169 Msa1364310:CDS 25.0%
! ATACATTTGACTAAAAAGGA+TGG - tig0018115:53154-53173 Msa1364310:CDS 25.0%
! ACACATTATTCATTAGATCA+TGG - tig0018115:53214-53233 Msa1364310:intron 25.0%
!! GAACAATCCAATTGAAATTT+TGG - tig0018115:53348-53367 Msa1364310:intron 25.0%
!! GAACTTGAAATTTGTGATTT+TGG + tig0018115:53403-53422 None:intergenic 25.0%
! TGTATGAGAAAAATTAGTAG+CGG - tig0018115:54004-54023 Msa1364310:intron 25.0%
! TTCTATTGATCATCTCAAAA+AGG + tig0018115:54078-54097 None:intergenic 25.0%
!!! CTCAAATTGATAGTGAAATT+TGG + tig0018115:54719-54738 None:intergenic 25.0%
! CATAAATACATATTCCTTAC+TGG + tig0018115:54767-54786 None:intergenic 25.0%
!! GTTTCTTTTACAGTTAGTTA+GGG + tig0018115:54892-54911 None:intergenic 25.0%
!! TGTTTCTTTTACAGTTAGTT+AGG + tig0018115:54893-54912 None:intergenic 25.0%
! TGTAGAAGAAAATAAATTGC+AGG - tig0018115:55072-55091 Msa1364310:intron 25.0%
TGAGACTGTACATATTCTTA+GGG + tig0018115:52655-52674 None:intergenic 30.0%
ACCTCAACAAATATTCAAGA+TGG - tig0018115:52707-52726 Msa1364310:CDS 30.0%
! TCCATCTTGAATATTTGTTG+AGG + tig0018115:52711-52730 None:intergenic 30.0%
!!! GTTGTTTGTTATGTTTTACG+CGG - tig0018115:52808-52827 Msa1364310:CDS 30.0%
!! TATTTCTCAGTGAAAGCATT+TGG + tig0018115:53076-53095 None:intergenic 30.0%
TATTTCCCATTCTTTCTCAT+CGG + tig0018115:53137-53156 None:intergenic 30.0%
CTAAAAAGGATGGTGATATT+TGG - tig0018115:53164-53183 Msa1364310:CDS 30.0%
ATGGTGATATTTGGATTCAA+AGG - tig0018115:53173-53192 Msa1364310:CDS 30.0%
TGGTGATATTTGGATTCAAA+GGG - tig0018115:53174-53193 Msa1364310:CDS 30.0%
TATGTAAACCTCAAAATGAG+AGG + tig0018115:53269-53288 None:intergenic 30.0%
GAAATTGAATATCGTGTTGA+CGG - tig0018115:53307-53326 Msa1364310:intron 30.0%
ACGGTAATAATCAACATGAA+CGG - tig0018115:53326-53345 Msa1364310:intron 30.0%
TGCTCATCCAAAATTTCAAT+TGG + tig0018115:53358-53377 None:intergenic 30.0%
! TTTAGGAATGTTTTGCGATT+TGG + tig0018115:53479-53498 None:intergenic 30.0%
! CTCTTCTGAGAAATTGAATA+TGG + tig0018115:53606-53625 None:intergenic 30.0%
CAAAATTTAAAGGGACATAC+TGG + tig0018115:53904-53923 None:intergenic 30.0%
! CTTTAAGGAAAAAACCATCT+TGG + tig0018115:54172-54191 None:intergenic 30.0%
TTCAACTTCAAATTCCCAAA+GGG + tig0018115:54359-54378 None:intergenic 30.0%
ATTCAACTTCAAATTCCCAA+AGG + tig0018115:54360-54379 None:intergenic 30.0%
! CTTAGCTTCAGAATTTTTGA+AGG + tig0018115:54660-54679 None:intergenic 30.0%
!!! GAAATTTGGTTTTTCCAAAG+TGG + tig0018115:54705-54724 None:intergenic 30.0%
! TCAATTTGAGATCAAGTCAT+TGG - tig0018115:54729-54748 Msa1364310:intron 30.0%
! CAATTTGAGATCAAGTCATT+GGG - tig0018115:54730-54749 Msa1364310:intron 30.0%
CTAGTGATTATGCTTAAAAC+TGG - tig0018115:54810-54829 Msa1364310:intron 30.0%
! AACATTGCAGTCTTCTTTTT+GGG + tig0018115:54965-54984 None:intergenic 30.0%
! TAACATTGCAGTCTTCTTTT+TGG + tig0018115:54966-54985 None:intergenic 30.0%
TACAGTTTGAGGAAAAATTG+TGG - tig0018115:54984-55003 Msa1364310:intron 30.0%
! CTTGATGTTCATATTTTCAG+AGG - tig0018115:55142-55161 Msa1364310:CDS 30.0%
GTGAGACTGTACATATTCTT+AGG + tig0018115:52656-52675 None:intergenic 35.0%
GAAGGAAAGAATGCTTACAA+GGG + tig0018115:52735-52754 None:intergenic 35.0%
AGAAGGAAAGAATGCTTACA+AGG + tig0018115:52736-52755 None:intergenic 35.0%
GCTTTCACTGAGAAATATAG+TGG - tig0018115:53079-53098 Msa1364310:CDS 35.0%
ATTTCCCATTCTTTCTCATC+GGG + tig0018115:53136-53155 None:intergenic 35.0%
GGTGATATTTGGATTCAAAG+GGG - tig0018115:53175-53194 Msa1364310:CDS 35.0%
ATATTTGGATTCAAAGGGGT+TGG - tig0018115:53179-53198 Msa1364310:intron 35.0%
TTCATTAGATCATGGTCACA+TGG - tig0018115:53222-53241 Msa1364310:intron 35.0%
! TCAGAAAACCTCTCATTTTG+AGG - tig0018115:53258-53277 Msa1364310:intron 35.0%
ATTGACACAAGTGAAGATGT+CGG - tig0018115:53445-53464 Msa1364310:intron 35.0%
!! CCTTTAACTTGGACAAGTTT+AGG + tig0018115:53496-53515 None:intergenic 35.0%
CCTAAACTTGTCCAAGTTAA+AGG - tig0018115:53493-53512 Msa1364310:CDS 35.0%
ATCATGACAGCAGTGAAAAA+GGG + tig0018115:53740-53759 None:intergenic 35.0%
TATCATGACAGCAGTGAAAA+AGG + tig0018115:53741-53760 None:intergenic 35.0%
! TGAGAAAAATTAGTAGCGGA+TGG - tig0018115:54008-54027 Msa1364310:intron 35.0%
TCATCCTTTGAATCAGTTCA+TGG - tig0018115:54235-54254 Msa1364310:intron 35.0%
! ATCTCCATGAACTGATTCAA+AGG + tig0018115:54242-54261 None:intergenic 35.0%
TTTGAATCAGTTCATGGAGA+TGG - tig0018115:54241-54260 Msa1364310:intron 35.0%
TTCATGGAGATGGAATTAAC+TGG - tig0018115:54251-54270 Msa1364310:intron 35.0%
ATTAACTGGTTAGAAACTGC+AGG - tig0018115:54265-54284 Msa1364310:intron 35.0%
ATCAAGCAGTATTGTCAAGA+TGG - tig0018115:54378-54397 Msa1364310:CDS 35.0%
CGTGAGTCGTAAAAAAATAG+AGG + tig0018115:54488-54507 None:intergenic 35.0%
AACTCAAAGAAATTCACTGC+AGG - tig0018115:54622-54641 Msa1364310:CDS 35.0%
!! TCAGAATTTTTGAAGGAGCA+TGG + tig0018115:54653-54672 None:intergenic 35.0%
CAAAGTGGATGTGAATTGAT+TGG + tig0018115:54690-54709 None:intergenic 35.0%
CAATCAATTCACATCCACTT+TGG - tig0018115:54688-54707 Msa1364310:CDS 35.0%
GATTATGCTTAAAACTGGAG+TGG - tig0018115:54815-54834 Msa1364310:intron 35.0%
GGATAATACATCACTTGTAC+AGG + tig0018115:55010-55029 None:intergenic 35.0%
GAAGAAAATAAATTGCAGGC+TGG - tig0018115:55076-55095 Msa1364310:CDS 35.0%
GTACATATTCTTAGGGTTGC+TGG + tig0018115:52648-52667 None:intergenic 40.0%
CTTCACATTCAGGCATTTAG+TGG + tig0018115:53639-53658 None:intergenic 40.0%
AAATGCCTGAATGTGAAGCA+TGG - tig0018115:53641-53660 Msa1364310:intron 40.0%
TCTTGGATGATGAGAGAACT+TGG - tig0018115:53969-53988 Msa1364310:intron 40.0%
AACCATCTTGGATTACCTTG+TGG + tig0018115:54160-54179 None:intergenic 40.0%
AAAGAAATTCACTGCAGGCT+AGG - tig0018115:54627-54646 Msa1364310:CDS 40.0%
TTCATTCTCGAGCAAGTACT+TGG - tig0018115:54937-54956 Msa1364310:intron 40.0%
TCATTCTCGAGCAAGTACTT+GGG - tig0018115:54938-54957 Msa1364310:intron 40.0%
GACTGCAATGTTACAGTTTG+AGG - tig0018115:54973-54992 Msa1364310:intron 40.0%
!! TGTTTTTGTGCTTGCCAACA+AGG - tig0018115:55108-55127 Msa1364310:CDS 40.0%
AAGCACAACATCTTCCTTGT+TGG + tig0018115:55125-55144 None:intergenic 40.0%
ATTCTTAGGGTTGCTGGTCA+TGG + tig0018115:52642-52661 None:intergenic 45.0%
GATTCCTTCCTTGTATCCTC+AGG - tig0018115:52832-52851 Msa1364310:CDS 45.0%
AGTTAGGTCCTGAGGATACA+AGG + tig0018115:52843-52862 None:intergenic 45.0%
TTCAAGTGAGTTAGGTCCTG+AGG + tig0018115:52851-52870 None:intergenic 45.0%
TCGGGAGTCTTGAGAAACAT+TGG + tig0018115:53118-53137 None:intergenic 45.0%
AGACTCCCGATGAGAAAGAA+TGG - tig0018115:53128-53147 Msa1364310:CDS 45.0%
GACTCCCGATGAGAAAGAAT+GGG - tig0018115:53129-53148 Msa1364310:CDS 45.0%
TTGGATTCAAAGGGGTTGGA+AGG - tig0018115:53183-53202 Msa1364310:intron 45.0%
GGTCTACTCTTCTTGTGAGA+AGG + tig0018115:53382-53401 None:intergenic 45.0%
CTTGACCATGCTTCACATTC+AGG + tig0018115:53649-53668 None:intergenic 45.0%
CTCATCATCCAAGACCTGAA+GGG + tig0018115:53963-53982 None:intergenic 45.0%
TCTCATCATCCAAGACCTGA+AGG + tig0018115:53964-53983 None:intergenic 45.0%
! TGGATTACCTTGTGGTGGAA+GGG + tig0018115:54152-54171 None:intergenic 45.0%
! TTGGATTACCTTGTGGTGGA+AGG + tig0018115:54153-54172 None:intergenic 45.0%
CATCTTGGATTACCTTGTGG+TGG + tig0018115:54157-54176 None:intergenic 45.0%
CACCACAAGGTAATCCAAGA+TGG - tig0018115:54155-54174 Msa1364310:CDS 45.0%
ATCGTTGTTGTGACTCCCTT+TGG - tig0018115:54341-54360 Msa1364310:CDS 45.0%
TCGTTGTTGTGACTCCCTTT+GGG - tig0018115:54342-54361 Msa1364310:CDS 45.0%
! TGCTTTTGTTGGCATACCTC+AGG - tig0018115:54590-54609 Msa1364310:CDS 45.0%
TTGAGTTTCCTTCGTTCCTG+AGG + tig0018115:54609-54628 None:intergenic 45.0%
GGGATGTTTACAGACCAGTA+AGG - tig0018115:54750-54769 Msa1364310:intron 45.0%
!! AACTTTGTTGCTGGTTCGGA+TGG + tig0018115:55031-55050 None:intergenic 45.0%
ATCCGAACCAGCAACAAAGT+TGG - tig0018115:55030-55049 Msa1364310:intron 45.0%
TCCGAACCAGCAACAAAGTT+GGG - tig0018115:55031-55050 Msa1364310:intron 45.0%
! ACCCAACTTTGTTGCTGGTT+CGG + tig0018115:55035-55054 None:intergenic 45.0%
CAACAAAGTTGGGTGAAGGT+TGG - tig0018115:55041-55060 Msa1364310:intron 45.0%
AGGTCCTGAGGATACAAGGA+AGG + tig0018115:52839-52858 None:intergenic 50.0%
TGAAGCATGGTCAAGAGCAG+AGG - tig0018115:53654-53673 Msa1364310:CDS 50.0%
CAACATTCCCTTCCACCACA+AGG - tig0018115:54142-54161 Msa1364310:CDS 50.0%
TTGGCATACCTCAGGAACGA+AGG - tig0018115:54598-54617 Msa1364310:CDS 50.0%
CCTTCACCCAACTTTGTTGC+TGG + tig0018115:55040-55059 None:intergenic 50.0%
CCAGCAACAAAGTTGGGTGA+AGG - tig0018115:55037-55056 Msa1364310:intron 50.0%
GCAAAGCGAAATCGCCCTTC+AGG - tig0018115:53946-53965 Msa1364310:intron 55.0%
CGAAATCGCCCTTCAGGTCT+TGG - tig0018115:53952-53971 Msa1364310:intron 55.0%
Chromosome Type Strat End Strand Name
tig0018115 gene 52635 55174 52635 ID=Msa1364310;Name=Msa1364310
tig0018115 mRNA 52635 55174 52635 ID=Msa1364310-mRNA-1;Parent=Msa1364310;Name=Msa1364310-mRNA-1;_AED=0.12;_eAED=0.12;_QI=0|0|0|1|1|1|7|0|484
tig0018115 exon 55073 55174 55073 ID=Msa1364310-mRNA-1:exon:13952;Parent=Msa1364310-mRNA-1
tig0018115 exon 54295 54742 54295 ID=Msa1364310-mRNA-1:exon:13951;Parent=Msa1364310-mRNA-1
tig0018115 exon 54025 54188 54025 ID=Msa1364310-mRNA-1:exon:13950;Parent=Msa1364310-mRNA-1
tig0018115 exon 53646 53904 53646 ID=Msa1364310-mRNA-1:exon:13949;Parent=Msa1364310-mRNA-1
tig0018115 exon 53481 53558 53481 ID=Msa1364310-mRNA-1:exon:13948;Parent=Msa1364310-mRNA-1
tig0018115 exon 53043 53197 53043 ID=Msa1364310-mRNA-1:exon:13947;Parent=Msa1364310-mRNA-1
tig0018115 exon 52635 52883 52635 ID=Msa1364310-mRNA-1:exon:13946;Parent=Msa1364310-mRNA-1
tig0018115 CDS 55073 55174 55073 ID=Msa1364310-mRNA-1:cds;Parent=Msa1364310-mRNA-1
tig0018115 CDS 54295 54742 54295 ID=Msa1364310-mRNA-1:cds;Parent=Msa1364310-mRNA-1
tig0018115 CDS 54025 54188 54025 ID=Msa1364310-mRNA-1:cds;Parent=Msa1364310-mRNA-1
tig0018115 CDS 53646 53904 53646 ID=Msa1364310-mRNA-1:cds;Parent=Msa1364310-mRNA-1
tig0018115 CDS 53481 53558 53481 ID=Msa1364310-mRNA-1:cds;Parent=Msa1364310-mRNA-1
tig0018115 CDS 53043 53197 53043 ID=Msa1364310-mRNA-1:cds;Parent=Msa1364310-mRNA-1
tig0018115 CDS 52635 52883 52635 ID=Msa1364310-mRNA-1:cds;Parent=Msa1364310-mRNA-1
Gene Sequence

>Msa1364310

ATGTCCATGACCAGCAACCCTAAGAATATGTACAGTCTCACTGCTTCTGCTATTCGTTTCTTTAAGATTGTTACCTCAACAAATATTCAAGATGGAACCCTTAGAATTCCAAATGCTTTCACTGAGAAATATAGTGGTGATTTATCAAAGCCAATGTTTCTCAAGACTCCCGATGAGAAAGAATGGGAAATACATTTGACTAAAAAGGATGGTGATATTTGGATTCAAAGGGGTTGGAAGGAATTTGCTACACATTATTCATTAGATCATGGTCACATGGTTGTGTTTCAATATCAGAAAACCTCTCATTTTGAGGTTTACATATTTGACAAGAGTACTTTTGAAATTGAATATCGTGTTGACGGTAATAATCAACATGAACGGAACAATCCAATTGAAATTTTGGATGAGCAACCTTCTCACAAGAAGAGTAGACCAAAATCACAAATTTCAAGTTCTCAACCACTTAAAAAATTGAGAATTGACACAAGTGAAGATGTCGGAACAAGCTCCAAATCGCAAAACATTCCTAAACTTGTCCAAGTTAAAGAAGAGATTGATTCTACCACTAAATGCCTGAATGTGAAGCATGGTCAAGAGCAGAGGAATTCAACTGCTAAAATTCTTGAAGCTTTAAACAAAGCAAAAAACTATGAATCTAACAATCCCTTTTTCACTGCTGTCATGATACATTCATATGTTAATCAATACATGTATGTCCCTTTAAATTTTGAACAAAAGTATTTGAAGGAACAGCAAAGCGAAATCGCCCTTCAGGTCTTGGATGATGAGAGAACTTGGATTGTTAAATATTGTATGAGAAAAATTAGTAGCGGATGGAAAACATTTGTATCTGATAATAATTTGAAATTGGGAGATGTTTGCCTTTTTGAGATGATCAATAGAAAATCCTATGCTTTTAAAGTACTAATTTTTCGAGTTGACGAAGAACAACATTCCCTTCCACCACAAGTTCATGGAGATGGAATTAACTGGTTAGAAACTGCAGGAATCACAGAAGTTGAAAGTAAAACTATTATGTCGTATAAAGGAACGAAGGAAACTCAAAGAAATTCACTGCAGGCTAGGCCATGCTCCTTCAAAAATTCTGAAGCTAAGAAAGAAGCCAATCAATTCACATCCACTTTGGAAAAACCAAATTTCACTATCAATTTGAGATCAAGTCATTGGGATGTTTACAGACCACGTGTACGAAATTCATTCTCGAGCAAGTACTTGGGTCCCAAAAAGAAGACTGCAATGTTACAGTTTGAGGAAAAATTGTGGCCTGTACAAGTGATGTATTATCCATCCGAACCAGCAACAAAGTTGGGTGAAGGTTGGAGTCTGTTTGTAGAAGAAAATAAATTGCAGGCTGGAGATGTATGTGTTTTTGTGCTTGCCAACAAGGAAGATGTTGTGCTTGATGTTCATATTTTCAGAGGACGCAGTTAG
Protein sequence

>Msa1364310

MSMTSNPKNMYSLTASAIRFFKIVTSTNIQDGTLRIPNAFTEKYSGDLSKPMFLKTPDEKEWEIHLTKKDGDIWIQRGWKEFATHYSLDHGHMVVFQYQKTSHFEVYIFDKSTFEIEYRVDGNNQHERNNPIEILDEQPSHKKSRPKSQISSSQPLKKLRIDTSEDVGTSSKSQNIPKLVQVKEEIDSTTKCLNVKHGQEQRNSTAKILEALNKAKNYESNNPFFTAVMIHSYVNQYMYVPLNFEQKYLKEQQSEIALQVLDDERTWIVKYCMRKISSGWKTFVSDNNLKLGDVCLFEMINRKSYAFKVLIFRVDEEQHSLPPQVHGDGINWLETAGITEVESKTIMSYKGTKETQRNSLQARPCSFKNSEAKKEANQFTSTLEKPNFTINLRSSHWDVYRPRVRNSFSSKYLGPKKKTAMLQFEEKLWPVQVMYYPSEPATKLGEGWSLFVEENKLQAGDVCVFVLANKEDVVLDVHIFRGRS*