AlfalfaGEDB | Msa1366240

M. sativa cultivar zhongmu-4 / Msa1366240

KEGG: -
GO: -
PFAM: -

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1366240	AES61090.1	88.811	143	16	0	12	154	3	145	6.12e-90	268

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1366240	G7I4V4	88.811	143	16	0	12	154	3	145	2.92e-90	268

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TFs/TRs:

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Gene ID	Type	Classification
Msa1366240	TF	C2H2

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1366240	MtrunA17_Chr1g0187511	88.811	143	16	0	12	154	3	145	5.63e-94	268
Msa1366240	MtrunA17_Chr1g0187701	41.667	144	76	4	17	154	15	156	1.75e-25	95.5
Msa1366240	MtrunA17_Chr1g0187541	43.791	153	69	7	9	154	4	146	1.18e-24	92.8
Msa1366240	MtrunA17_Chr4g0031161	35.057	174	73	5	17	154	13	182	1.31e-20	83.2
Msa1366240	MtrunA17_Chr5g0423491	35.333	150	78	6	1	142	1	139	4.26e-15	68.2
Msa1366240	MtrunA17_Chr1g0166461	30.380	158	90	4	14	154	13	167	7.64e-15	67.8

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Find 28 sgRNAs with CRISPR-Local

Find 37 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAATTGGGTGGTAAAGGGT+TGG	0.523418	tig0018446:+99603	None:intergenic
AAATTGGGTGGTAAAGGGTT+GGG	0.489112	tig0018446:+99604	None:intergenic
AAGCCATCTTGAGTCTCATA+TGG	0.473825	tig0018446:-99686	Msa1366240:CDS
ACAACATTAGATTGTTGTTG+TGG	0.560718	tig0018446:+99520	None:intergenic
ACAATCTAATGTTGTTGATG+TGG	0.605335	tig0018446:-99512	Msa1366240:CDS
ATGCACCAACAAGGGACTGG+TGG	0.640658	tig0018446:-99466	Msa1366240:CDS
ATGGAAGTCTCTCCGATCGA+TGG	0.567201	tig0018446:-99442	Msa1366240:CDS
ATGGGAAAATTGGGTGGTAA+AGG	0.416030	tig0018446:+99598	None:intergenic
ATTAAACCATACCAATCCTC+AGG	0.560036	tig0018446:-99638	Msa1366240:CDS
ATTCGGCCTGAGGATTGGTA+TGG	0.511741	tig0018446:+99632	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
ACAATCTAATGTTGTTGATG+TGG	-	tig0018446:99614-99633	Msa1366240:CDS	30.0%
TAAGCCATATATCAACATGT+TGG	-	tig0018446:99710-99729	Msa1366240:CDS	30.0%
ATTAAACCATACCAATCCTC+AGG	-	tig0018446:99488-99507	Msa1366240:CDS	35.0%
CAATGACACATCAGTGAATT+TGG	-	tig0018446:99770-99789	Msa1366240:CDS	35.0%
AAGCCATCTTGAGTCTCATA+TGG	-	tig0018446:99440-99459	Msa1366240:CDS	40.0%
AAATTGGGTGGTAAAGGGTT+GGG	+	tig0018446:99525-99544	None:intergenic	40.0%
AAAATTGGGTGGTAAAGGGT+TGG	+	tig0018446:99526-99545	None:intergenic	40.0%
TGGGAAAATTGGGTGGTAAA+GGG	+	tig0018446:99530-99549	None:intergenic	40.0%
ATGGGAAAATTGGGTGGTAA+AGG	+	tig0018446:99531-99550	None:intergenic	40.0%
TATGGCTTAGTTCCATCGAT+CGG	+	tig0018446:99699-99718	None:intergenic	40.0%

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Chromosome	Type	Strat	End	Strand	Name
tig0018446	gene	99342	99806	99342	ID=Msa1366240;Name=Msa1366240
tig0018446	mRNA	99342	99806	99342	ID=Msa1366240-mRNA-1;Parent=Msa1366240;Name=Msa1366240-mRNA-1;_AED=0.04;_eAED=0.04;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|154
tig0018446	exon	99342	99806	99342	ID=Msa1366240-mRNA-1:exon:15032;Parent=Msa1366240-mRNA-1
tig0018446	CDS	99342	99806	99342	ID=Msa1366240-mRNA-1:cds;Parent=Msa1366240-mRNA-1

Gene Sequence

>Msa1366240

ATGGGTGACGATTTCGCTCAAGAGAACAACCGTAGCAATATCAATAATTTTGTATGTGGATTGTGCAATCATGATTGCACAAACTTCGAATCACTCATAAGCCATCTTGAGTCTCATATGGCTCGAGAAAATCTTGCTATTGCAAGATTAAACCATACCAATCCTCAGGCCGAATCAATGCCCAACCCTTTACCACCCAATTTTCCCATGCCAAAATCATTACATGAAAACAAAACCTTCGTTAAAAGCAAGTTATTTCAGCAACCACAACAACAATCTAATGTTGTTGATGTGGCTTCATTTTCAGCTCCACCATCAATGCACCAACAAGGGACTGGTGGTATGGAAGTCTCTCCGATCGATGGAACTAAGCCATATATCAACATGTTGGATAAACCCATCGAGAAAAATGTCTTCATTAACATCATCAATGACACATCAGTGAATTTGGAGTTAAAGCTCTAG

Protein sequence

>Msa1366240

MGDDFAQENNRSNINNFVCGLCNHDCTNFESLISHLESHMARENLAIARLNHTNPQAESMPNPLPPNFPMPKSLHENKTFVKSKLFQQPQQQSNVVDVASFSAPPSMHQQGTGGMEVSPIDGTKPYINMLDKPIEKNVFINIINDTSVNLELKL*