Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1366240 | AES61090.1 | 88.811 | 143 | 16 | 0 | 12 | 154 | 3 | 145 | 6.12e-90 | 268 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1366240 | G7I4V4 | 88.811 | 143 | 16 | 0 | 12 | 154 | 3 | 145 | 2.92e-90 | 268 |
| Gene ID | Type | Classification |
|---|---|---|
| Msa1366240 | TF | C2H2 |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1366240 | MtrunA17_Chr1g0187511 | 88.811 | 143 | 16 | 0 | 12 | 154 | 3 | 145 | 5.63e-94 | 268 |
| Msa1366240 | MtrunA17_Chr1g0187701 | 41.667 | 144 | 76 | 4 | 17 | 154 | 15 | 156 | 1.75e-25 | 95.5 |
| Msa1366240 | MtrunA17_Chr1g0187541 | 43.791 | 153 | 69 | 7 | 9 | 154 | 4 | 146 | 1.18e-24 | 92.8 |
| Msa1366240 | MtrunA17_Chr4g0031161 | 35.057 | 174 | 73 | 5 | 17 | 154 | 13 | 182 | 1.31e-20 | 83.2 |
| Msa1366240 | MtrunA17_Chr5g0423491 | 35.333 | 150 | 78 | 6 | 1 | 142 | 1 | 139 | 4.26e-15 | 68.2 |
| Msa1366240 | MtrunA17_Chr1g0166461 | 30.380 | 158 | 90 | 4 | 14 | 154 | 13 | 167 | 7.64e-15 | 67.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 28 sgRNAs with CRISPR-Local
Find 37 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CAATGACACATCAGTGAATT+TGG | 0.236191 | tig0018446:-99356 | Msa1366240:CDS |
| TTATCCAACATGTTGATATA+TGG | 0.260832 | tig0018446:+99412 | None:intergenic |
| GTCCCTTGTTGGTGCATTGA+TGG | 0.334927 | tig0018446:+99472 | None:intergenic |
| CCATACCACCAGTCCCTTGT+TGG | 0.378244 | tig0018446:+99461 | None:intergenic |
| ATGGGAAAATTGGGTGGTAA+AGG | 0.416030 | tig0018446:+99598 | None:intergenic |
| TAAAGGGTTGGGCATTGATT+CGG | 0.452189 | tig0018446:+99615 | None:intergenic |
| TGGGAAAATTGGGTGGTAAA+GGG | 0.468596 | tig0018446:+99599 | None:intergenic |
| CATTGATTCGGCCTGAGGAT+TGG | 0.472364 | tig0018446:+99627 | None:intergenic |
| AAGCCATCTTGAGTCTCATA+TGG | 0.473825 | tig0018446:-99686 | Msa1366240:CDS |
| CCAACAAGGGACTGGTGGTA+TGG | 0.486031 | tig0018446:-99461 | Msa1366240:CDS |
| AAATTGGGTGGTAAAGGGTT+GGG | 0.489112 | tig0018446:+99604 | None:intergenic |
| CAAAATTATTGATATTGCTA+CGG | 0.495293 | tig0018446:+99755 | None:intergenic |
| ATTCGGCCTGAGGATTGGTA+TGG | 0.511741 | tig0018446:+99632 | None:intergenic |
| AAAATTGGGTGGTAAAGGGT+TGG | 0.523418 | tig0018446:+99603 | None:intergenic |
| TTGGGCATTGATTCGGCCTG+AGG | 0.534860 | tig0018446:+99622 | None:intergenic |
| CCTTGTTGGTGCATTGATGG+TGG | 0.554951 | tig0018446:+99475 | None:intergenic |
| ATTAAACCATACCAATCCTC+AGG | 0.560036 | tig0018446:-99638 | Msa1366240:CDS |
| ACAACATTAGATTGTTGTTG+TGG | 0.560718 | tig0018446:+99520 | None:intergenic |
| ATGGAAGTCTCTCCGATCGA+TGG | 0.567201 | tig0018446:-99442 | Msa1366240:CDS |
| TAAGCCATATATCAACATGT+TGG | 0.587500 | tig0018446:-99416 | Msa1366240:CDS |
| TCAATGCACCAACAAGGGAC+TGG | 0.598187 | tig0018446:-99469 | Msa1366240:CDS |
| TTTGGCATGGGAAAATTGGG+TGG | 0.600647 | tig0018446:+99592 | None:intergenic |
| CCACCATCAATGCACCAACA+AGG | 0.600697 | tig0018446:-99475 | Msa1366240:CDS |
| ACAATCTAATGTTGTTGATG+TGG | 0.605335 | tig0018446:-99512 | Msa1366240:CDS |
| GAGCCATATGAGACTCAAGA+TGG | 0.605655 | tig0018446:+99683 | None:intergenic |
| CACCATCAATGCACCAACAA+GGG | 0.620275 | tig0018446:-99474 | Msa1366240:CDS |
| TATGGCTTAGTTCCATCGAT+CGG | 0.625287 | tig0018446:+99430 | None:intergenic |
| ATGCACCAACAAGGGACTGG+TGG | 0.640658 | tig0018446:-99466 | Msa1366240:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AATATCAATAATTTTGTATG+TGG | - | tig0018446:99378-99397 | Msa1366240:CDS | 15.0% |
| !! | CAAAATTATTGATATTGCTA+CGG | + | tig0018446:99374-99393 | None:intergenic | 20.0% |
| !!! | TTGTTTTCATGTAATGATTT+TGG | + | tig0018446:99555-99574 | None:intergenic | 20.0% |
| !!! | AAATAACTTGCTTTTAACGA+AGG | + | tig0018446:99580-99599 | None:intergenic | 25.0% |
| ! | TTATCCAACATGTTGATATA+TGG | + | tig0018446:99717-99736 | None:intergenic | 25.0% |
| !! | TCATGTAATGATTTTGGCAT+GGG | + | tig0018446:99549-99568 | None:intergenic | 30.0% |
| !! | TTCATGTAATGATTTTGGCA+TGG | + | tig0018446:99550-99569 | None:intergenic | 30.0% |
| ! | ACAACATTAGATTGTTGTTG+TGG | + | tig0018446:99609-99628 | None:intergenic | 30.0% |
| ACAATCTAATGTTGTTGATG+TGG | - | tig0018446:99614-99633 | Msa1366240:CDS | 30.0% | |
| TAAGCCATATATCAACATGT+TGG | - | tig0018446:99710-99729 | Msa1366240:CDS | 30.0% | |
| ! | ATGAAGACATTTTTCTCGAT+GGG | + | tig0018446:99741-99760 | None:intergenic | 30.0% |
| ! | AATGAAGACATTTTTCTCGA+TGG | + | tig0018446:99742-99761 | None:intergenic | 30.0% |
| ATTAAACCATACCAATCCTC+AGG | - | tig0018446:99488-99507 | Msa1366240:CDS | 35.0% | |
| !! | GATTTTGGCATGGGAAAATT+GGG | + | tig0018446:99540-99559 | None:intergenic | 35.0% |
| !! | TGATTTTGGCATGGGAAAAT+TGG | + | tig0018446:99541-99560 | None:intergenic | 35.0% |
| CAATGACACATCAGTGAATT+TGG | - | tig0018446:99770-99789 | Msa1366240:CDS | 35.0% | |
| AAGCCATCTTGAGTCTCATA+TGG | - | tig0018446:99440-99459 | Msa1366240:CDS | 40.0% | |
| !! | TAAAGGGTTGGGCATTGATT+CGG | + | tig0018446:99514-99533 | None:intergenic | 40.0% |
| AAATTGGGTGGTAAAGGGTT+GGG | + | tig0018446:99525-99544 | None:intergenic | 40.0% | |
| AAAATTGGGTGGTAAAGGGT+TGG | + | tig0018446:99526-99545 | None:intergenic | 40.0% | |
| TGGGAAAATTGGGTGGTAAA+GGG | + | tig0018446:99530-99549 | None:intergenic | 40.0% | |
| ATGGGAAAATTGGGTGGTAA+AGG | + | tig0018446:99531-99550 | None:intergenic | 40.0% | |
| TATGGCTTAGTTCCATCGAT+CGG | + | tig0018446:99699-99718 | None:intergenic | 40.0% | |
| GAGCCATATGAGACTCAAGA+TGG | + | tig0018446:99446-99465 | None:intergenic | 45.0% | |
| TTTGGCATGGGAAAATTGGG+TGG | + | tig0018446:99537-99556 | None:intergenic | 45.0% | |
| CACCATCAATGCACCAACAA+GGG | - | tig0018446:99652-99671 | Msa1366240:CDS | 45.0% | |
| ! | ATTCGGCCTGAGGATTGGTA+TGG | + | tig0018446:99497-99516 | None:intergenic | 50.0% |
| !! | CATTGATTCGGCCTGAGGAT+TGG | + | tig0018446:99502-99521 | None:intergenic | 50.0% |
| ! | CCTTGTTGGTGCATTGATGG+TGG | + | tig0018446:99654-99673 | None:intergenic | 50.0% |
| CCACCATCAATGCACCAACA+AGG | - | tig0018446:99651-99670 | Msa1366240:CDS | 50.0% | |
| GTCCCTTGTTGGTGCATTGA+TGG | + | tig0018446:99657-99676 | None:intergenic | 50.0% | |
| TCAATGCACCAACAAGGGAC+TGG | - | tig0018446:99657-99676 | Msa1366240:CDS | 50.0% | |
| ATGGAAGTCTCTCCGATCGA+TGG | - | tig0018446:99684-99703 | Msa1366240:CDS | 50.0% | |
| !! | TTGGGCATTGATTCGGCCTG+AGG | + | tig0018446:99507-99526 | None:intergenic | 55.0% |
| ATGCACCAACAAGGGACTGG+TGG | - | tig0018446:99660-99679 | Msa1366240:CDS | 55.0% | |
| CCATACCACCAGTCCCTTGT+TGG | + | tig0018446:99668-99687 | None:intergenic | 55.0% | |
| ! | CCAACAAGGGACTGGTGGTA+TGG | - | tig0018446:99665-99684 | Msa1366240:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| tig0018446 | gene | 99342 | 99806 | 99342 | ID=Msa1366240;Name=Msa1366240 |
| tig0018446 | mRNA | 99342 | 99806 | 99342 | ID=Msa1366240-mRNA-1;Parent=Msa1366240;Name=Msa1366240-mRNA-1;_AED=0.04;_eAED=0.04;_QI=0|-1|0|1|-1|1|1|0|154 |
| tig0018446 | exon | 99342 | 99806 | 99342 | ID=Msa1366240-mRNA-1:exon:15032;Parent=Msa1366240-mRNA-1 |
| tig0018446 | CDS | 99342 | 99806 | 99342 | ID=Msa1366240-mRNA-1:cds;Parent=Msa1366240-mRNA-1 |
| Gene Sequence |
| Protein sequence |
