Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1366280 | RHN80374.1 | 89.116 | 147 | 15 | 1 | 1 | 147 | 1 | 146 | 7.56e-90 | 268 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1366280 | A0A396JSX4 | 89.116 | 147 | 15 | 1 | 1 | 147 | 1 | 146 | 3.61e-90 | 268 |
| Gene ID | Type | Classification |
|---|---|---|
| Msa1366280 | TF | C2H2 |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1366280 | MtrunA17_Chr1g0187541 | 89.116 | 147 | 15 | 1 | 1 | 147 | 1 | 146 | 6.95e-94 | 268 |
| Msa1366280 | MtrunA17_Chr1g0187511 | 42.177 | 147 | 66 | 7 | 11 | 147 | 8 | 145 | 1.41e-23 | 89.7 |
| Msa1366280 | MtrunA17_Chr4g0031161 | 36.047 | 172 | 73 | 6 | 11 | 147 | 13 | 182 | 6.21e-23 | 89.0 |
| Msa1366280 | MtrunA17_Chr1g0187701 | 36.810 | 163 | 69 | 10 | 11 | 147 | 15 | 169 | 6.96e-19 | 78.2 |
| Msa1366280 | MtrunA17_Chr1g0166461 | 34.375 | 160 | 78 | 7 | 9 | 147 | 14 | 167 | 6.21e-13 | 62.8 |
| Msa1366280 | MtrunA17_Chr5g0423491 | 32.576 | 132 | 74 | 3 | 5 | 128 | 10 | 134 | 5.09e-11 | 57.4 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 31 sgRNAs with CRISPR-Local
Find 32 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTATCTAGTTTATTGATATA+TGG | 0.244365 | tig0018446:-136125 | None:intergenic |
| TGCATTTCACTAAACATTCT+TGG | 0.273305 | tig0018446:-135960 | None:intergenic |
| ACCAACCCATGAAGATTATA+TGG | 0.323053 | tig0018446:+136076 | Msa1366280:CDS |
| GCACATTGAATCTAGAATTA+AGG | 0.324100 | tig0018446:+136195 | Msa1366280:CDS |
| TAATGTTGGTTAGCCTGCTT+AGG | 0.325076 | tig0018446:-135899 | None:intergenic |
| AAACTTCCATATAATCTTCA+TGG | 0.356028 | tig0018446:-136082 | None:intergenic |
| TCGATTTCTCATAACTCATA+TGG | 0.358118 | tig0018446:+135848 | Msa1366280:CDS |
| ATGGAAGTTTCTCACATTGA+TGG | 0.390703 | tig0018446:+136095 | Msa1366280:CDS |
| TCTGCTGAGGAAAATGTTGC+TGG | 0.421474 | tig0018446:+136032 | Msa1366280:CDS |
| AAAGGTGGTGCTTCAATATG+AGG | 0.426497 | tig0018446:-135924 | None:intergenic |
| AAGAAAATTTAGCCCTAAGC+AGG | 0.461361 | tig0018446:+135886 | Msa1366280:CDS |
| AACTCATATGGAGTCTCATA+TGG | 0.462152 | tig0018446:+135860 | Msa1366280:CDS |
| TCCATATAATCTTCATGGGT+TGG | 0.462544 | tig0018446:-136077 | None:intergenic |
| AATGTTGGTTAGCCTGCTTA+GGG | 0.479077 | tig0018446:-135898 | None:intergenic |
| CCATCATTGATCACAATAGC+TGG | 0.489184 | tig0018446:-136173 | None:intergenic |
| TCTTCATGGGTTGGTGGAGT+TGG | 0.495187 | tig0018446:-136068 | None:intergenic |
| CCAGCTATTGTGATCAATGA+TGG | 0.501173 | tig0018446:+136173 | Msa1366280:CDS |
| GCTATCATTATTGTTAGCCA+TGG | 0.507658 | tig0018446:-135778 | None:intergenic |
| ATTATCAATAATCTTATCGA+TGG | 0.515635 | tig0018446:-136150 | None:intergenic |
| TGAGGAAAATGTTGCTGGAA+TGG | 0.518703 | tig0018446:+136037 | Msa1366280:CDS |
| TCACTAAACATTCTTGGTCG+AGG | 0.522107 | tig0018446:-135954 | None:intergenic |
| AACTTCCATATAATCTTCAT+GGG | 0.524548 | tig0018446:-136081 | None:intergenic |
| TTCAATATGAGGGTTAATGT+TGG | 0.536185 | tig0018446:-135913 | None:intergenic |
| CTTGGTCGAGGTATTGTCAA+AGG | 0.560141 | tig0018446:-135942 | None:intergenic |
| TTATCAATAATCTTATCGAT+GGG | 0.560338 | tig0018446:-136149 | None:intergenic |
| ATACCATGTTGTAAAATATG+AGG | 0.569652 | tig0018446:-136005 | None:intergenic |
| ATATAATCTTCATGGGTTGG+TGG | 0.585977 | tig0018446:-136074 | None:intergenic |
| ACATGGTATGCCATCTGCTG+AGG | 0.623247 | tig0018446:+136019 | Msa1366280:CDS |
| GGTCGAGGTATTGTCAAAGG+TGG | 0.641328 | tig0018446:-135939 | None:intergenic |
| AAGGTGGTGCTTCAATATGA+GGG | 0.646397 | tig0018446:-135923 | None:intergenic |
| CCATGTTGTAAAATATGAGG+TGG | 0.673172 | tig0018446:-136002 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TTATCTAGTTTATTGATATA+TGG | - | tig0018446:136128-136147 | None:intergenic | 15.0% |
| !! | TTATCAATAATCTTATCGAT+GGG | - | tig0018446:136152-136171 | None:intergenic | 20.0% |
| !! | ATTATCAATAATCTTATCGA+TGG | - | tig0018446:136153-136172 | None:intergenic | 20.0% |
| ! | ATACCATGTTGTAAAATATG+AGG | - | tig0018446:136008-136027 | None:intergenic | 25.0% |
| ! | AACTTCCATATAATCTTCAT+GGG | - | tig0018446:136084-136103 | None:intergenic | 25.0% |
| ! | AAACTTCCATATAATCTTCA+TGG | - | tig0018446:136085-136104 | None:intergenic | 25.0% |
| TCGATTTCTCATAACTCATA+TGG | + | tig0018446:135848-135867 | Msa1366280:CDS | 30.0% | |
| TTCAATATGAGGGTTAATGT+TGG | - | tig0018446:135916-135935 | None:intergenic | 30.0% | |
| TGCATTTCACTAAACATTCT+TGG | - | tig0018446:135963-135982 | None:intergenic | 30.0% | |
| ! | GCACATTGAATCTAGAATTA+AGG | + | tig0018446:136195-136214 | Msa1366280:CDS | 30.0% |
| AACTCATATGGAGTCTCATA+TGG | + | tig0018446:135860-135879 | Msa1366280:CDS | 35.0% | |
| AAGAAAATTTAGCCCTAAGC+AGG | + | tig0018446:135886-135905 | Msa1366280:CDS | 35.0% | |
| CCATGTTGTAAAATATGAGG+TGG | - | tig0018446:136005-136024 | None:intergenic | 35.0% | |
| ! | CCACCTCATATTTTACAACA+TGG | + | tig0018446:136002-136021 | Msa1366280:CDS | 35.0% |
| !! | ATATAATCTTCATGGGTTGG+TGG | - | tig0018446:136077-136096 | None:intergenic | 35.0% |
| ACCAACCCATGAAGATTATA+TGG | + | tig0018446:136076-136095 | Msa1366280:CDS | 35.0% | |
| TCCATATAATCTTCATGGGT+TGG | - | tig0018446:136080-136099 | None:intergenic | 35.0% | |
| ATGGAAGTTTCTCACATTGA+TGG | + | tig0018446:136095-136114 | Msa1366280:CDS | 35.0% | |
| !! | AATGTTGGTTAGCCTGCTTA+GGG | - | tig0018446:135901-135920 | None:intergenic | 40.0% |
| !! | TAATGTTGGTTAGCCTGCTT+AGG | - | tig0018446:135902-135921 | None:intergenic | 40.0% |
| AAGGTGGTGCTTCAATATGA+GGG | - | tig0018446:135926-135945 | None:intergenic | 40.0% | |
| AAAGGTGGTGCTTCAATATG+AGG | - | tig0018446:135927-135946 | None:intergenic | 40.0% | |
| TCACTAAACATTCTTGGTCG+AGG | - | tig0018446:135957-135976 | None:intergenic | 40.0% | |
| TGAGGAAAATGTTGCTGGAA+TGG | + | tig0018446:136037-136056 | Msa1366280:CDS | 40.0% | |
| CCATCATTGATCACAATAGC+TGG | - | tig0018446:136176-136195 | None:intergenic | 40.0% | |
| CCAGCTATTGTGATCAATGA+TGG | + | tig0018446:136173-136192 | Msa1366280:CDS | 40.0% | |
| !! | CTTGGTCGAGGTATTGTCAA+AGG | - | tig0018446:135945-135964 | None:intergenic | 45.0% |
| ! | GCAACATTTTCCTCAGCAGA+TGG | - | tig0018446:136032-136051 | None:intergenic | 45.0% |
| TCTGCTGAGGAAAATGTTGC+TGG | + | tig0018446:136032-136051 | Msa1366280:CDS | 45.0% | |
| !! | GGTCGAGGTATTGTCAAAGG+TGG | - | tig0018446:135942-135961 | None:intergenic | 50.0% |
| ACATGGTATGCCATCTGCTG+AGG | + | tig0018446:136019-136038 | Msa1366280:CDS | 50.0% | |
| !! | TCTTCATGGGTTGGTGGAGT+TGG | - | tig0018446:136071-136090 | None:intergenic | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| tig0018446 | gene | 135780 | 136223 | 135780 | ID=Msa1366280;Name=Msa1366280 |
| tig0018446 | mRNA | 135780 | 136223 | 135780 | ID=Msa1366280-mRNA-1;Parent=Msa1366280;Name=Msa1366280-mRNA-1;_AED=0.04;_eAED=0.04;_QI=0|-1|0|1|-1|1|1|0|147 |
| tig0018446 | exon | 135780 | 136223 | 135780 | ID=Msa1366280-mRNA-1:exon:15026;Parent=Msa1366280-mRNA-1 |
| tig0018446 | CDS | 135780 | 136223 | 135780 | ID=Msa1366280-mRNA-1:cds;Parent=Msa1366280-mRNA-1 |
| Gene Sequence |
| Protein sequence |
