AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1366310

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1366310 MtrunA17_Chr1g0187591 82.083 653 56 3 1 592 1 653 0.0 1029
Msa1366310 MtrunA17_Chr3g0105131 69.081 566 113 7 1 564 1 506 0.0 718
Msa1366310 MtrunA17_Chr5g0430211 53.744 681 211 18 1 591 1 667 0.0 622
Msa1366310 MtrunA17_Chr3g0105101 71.663 427 64 6 37 460 29 401 0.0 582
Msa1366310 MtrunA17_Chr5g0437251 46.012 652 247 14 19 586 13 643 0.0 532
Msa1366310 MtrunA17_Chr7g0257831 41.096 657 261 16 33 586 47 680 2.71e-144 434
Msa1366310 MtrunA17_Chr3g0113651 44.965 576 196 19 31 583 8 485 2.52e-143 425
Msa1366310 MtrunA17_Chr3g0088521 38.815 675 268 21 30 588 42 687 4.60e-130 398
Msa1366310 MtrunA17_Chr3g0105121 52.547 373 142 7 222 582 8 357 6.86e-112 338
Msa1366310 MtrunA17_Chr1g0171151 41.575 457 229 10 33 451 31 487 1.94e-102 322
Msa1366310 MtrunA17_Chr3g0092171 46.260 361 163 7 30 374 80 425 4.68e-94 297
Msa1366310 MtrunA17_Chr3g0105141 66.434 143 47 1 188 329 1 143 6.53e-68 218
Msa1366310 MtrunA17_Chr3g0105151 57.522 226 79 6 383 591 1 226 1.41e-67 219
Msa1366310 MtrunA17_Chr3g0092221 44.664 253 110 4 119 346 34 281 5.05e-66 221
Msa1366310 MtrunA17_Chr8g0389561 43.668 229 110 4 219 428 77 305 4.49e-55 191
Msa1366310 MtrunA17_Chr6g0487481 70.093 107 32 0 219 325 99 205 2.71e-53 186
Msa1366310 MtrunA17_Chr3g0135351 54.037 161 62 4 187 344 25 176 4.29e-51 177
Msa1366310 MtrunA17_Chr2g0324781 62.162 111 42 0 223 333 77 187 1.49e-48 174
Msa1366310 MtrunA17_Chr4g0040391 57.944 107 45 0 219 325 56 162 1.65e-45 163
Msa1366310 MtrunA17_Chr3g0105111 71.739 92 13 1 501 592 3 81 9.55e-37 132
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1366310 AT5G58620.1 49.668 602 245 17 21 583 2 584 0.0 529
Msa1366310 AT3G55980.1 46.912 599 250 15 1 583 1 547 8.34e-171 498
Msa1366310 AT3G55980.2 46.912 599 250 15 1 583 68 614 6.32e-170 498
Msa1366310 AT2G40140.2 47.333 600 254 17 1 583 1 555 6.48e-169 493
Msa1366310 AT2G40140.1 47.333 600 254 17 1 583 1 555 6.48e-169 493
Msa1366310 AT5G12850.1 41.667 612 262 18 25 548 34 638 1.43e-137 417
Msa1366310 AT2G41900.1 47.222 468 186 8 25 447 40 491 6.88e-134 407
Msa1366310 AT4G29190.1 40.000 285 123 9 192 464 49 297 1.08e-55 192
Msa1366310 AT2G19810.1 55.195 154 52 3 208 359 61 199 2.76e-52 183
Msa1366310 AT5G07500.1 60.331 121 48 0 205 325 34 154 5.24e-52 179
Msa1366310 AT2G25900.2 58.333 132 55 0 219 350 86 217 8.32e-52 181
Msa1366310 AT2G25900.1 58.333 132 55 0 219 350 86 217 8.32e-52 181
Msa1366310 AT1G03790.1 52.258 155 66 3 190 340 53 203 1.01e-48 174
Msa1366310 AT5G44260.1 57.812 128 49 2 201 327 42 165 2.48e-46 167

Find 152 sgRNAs with CRISPR-Local

Find 164 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTAAGAGTATCTTCGGTTTA+AGG 0.141521 tig0018446:+167852 Msa1366310:CDS
CTTGTCAAACTGGTTTGATT+AGG 0.230886 tig0018446:+167405 Msa1366310:CDS
GGTGTGTTATCTTATATTCT+TGG 0.264718 tig0018446:+167647 Msa1366310:CDS
ATGAGGAAAACTTATTCTTT+TGG 0.268225 tig0018446:+169000 Msa1366310:CDS
GAACGCCATTATATCATCTT+TGG 0.288121 tig0018446:-167492 None:intergenic
TTGTCAAACTGGTTTGATTA+GGG 0.297404 tig0018446:+167406 Msa1366310:CDS
GAACTTGAAAATCGCCTTAC+AGG 0.298409 tig0018446:+168631 Msa1366310:CDS
ACGTTCTGCCTTCTCTGGTT+GGG 0.305180 tig0018446:+168924 Msa1366310:CDS
GTGAATTAACCCATGATTCA+TGG 0.308397 tig0018446:-169095 None:intergenic
TATATAGTACAGATGAATTT+AGG 0.309340 tig0018446:+168032 Msa1366310:CDS
GACGATTTCCTTAAGAAAAT+AGG 0.314739 tig0018446:+167911 Msa1366310:CDS
CTTGTTGTTGCTCCACCATT+TGG 0.317761 tig0018446:-167937 None:intergenic
CTTATGGTTGCTGCTTTGTT+TGG 0.322501 tig0018446:+167617 Msa1366310:CDS
GAGCACAAACCAGCAACTTT+GGG 0.343665 tig0018446:+168750 Msa1366310:CDS
AAAAGAAAACCTTGTCAAAC+TGG 0.347204 tig0018446:+167395 Msa1366310:CDS
GTGTCCCTTGCCCCGAGTTT+CGG 0.352020 tig0018446:+168176 Msa1366310:CDS
TGTTGATCAAATTCCATTGT+TGG 0.357494 tig0018446:-169132 None:intergenic
CAAGGGCTTATTCTCATGAT+TGG 0.357705 tig0018446:+168083 Msa1366310:CDS
TCTCCTTCAAATAGGCTTGC+AGG 0.358629 tig0018446:+168661 Msa1366310:CDS
AAGGATTGCCCCAAAGTTGC+TGG 0.360358 tig0018446:-168759 None:intergenic
TTCAGCCAAAGATGATATAA+TGG 0.367048 tig0018446:+167487 Msa1366310:CDS
CTTACCAATTCAAATGTGAT+TGG 0.367908 tig0018446:+168787 Msa1366310:CDS
TGATCCAATCACATTTGAAT+TGG 0.381273 tig0018446:-168791 None:intergenic
AGAGCACAAACCAGCAACTT+TGG 0.387067 tig0018446:+168749 Msa1366310:CDS
TGCTGCGCTCAATAAAGCTT+TGG 0.390375 tig0018446:-168849 None:intergenic
TTATCTTATATTCTTGGAAC+CGG 0.390464 tig0018446:+167653 Msa1366310:CDS
GAAGGTTGGATCAAAAGAAA+TGG 0.391384 tig0018446:+167574 Msa1366310:CDS
AAATTCACCAAGTGCTTCTT+CGG 0.396196 tig0018446:+168405 Msa1366310:CDS
TGAAGAGAGGACACCTCTTA+TGG 0.399251 tig0018446:+167601 Msa1366310:CDS
AACGTTCTGCCTTCTCTGGT+TGG 0.399281 tig0018446:+168923 Msa1366310:CDS
GTCTTGCATCTCCTTCAAAT+AGG 0.399881 tig0018446:+168653 Msa1366310:CDS
CCGCTGATGCAAACGGTAAC+CGG 0.400035 tig0018446:+167804 Msa1366310:CDS
ATGGAACGTTCTGCCTTCTC+TGG 0.406240 tig0018446:+168919 Msa1366310:CDS
TTTGAATTGGTAAGATCAAA+AGG 0.409476 tig0018446:-168778 None:intergenic
TCAAAGTGAAGCCTTGTTCA+AGG 0.431149 tig0018446:+168065 Msa1366310:CDS
TGCTGCTTTGTTTGGAAGCA+AGG 0.432478 tig0018446:+167625 Msa1366310:CDS
TCTGGTTGGGGCTCTCCTGA+TGG 0.432980 tig0018446:+168937 Msa1366310:CDS
GAGTGCCCCTTTGTTCAACC+TGG 0.438001 tig0018446:+168109 Msa1366310:CDS
CGTTCTGCCTTCTCTGGTTG+GGG 0.438457 tig0018446:+168925 Msa1366310:CDS
TTCTCCCCAGGTTGAACAAA+GGG 0.438980 tig0018446:-168115 None:intergenic
TATATTCCATTCTTTATGTC+AGG 0.439563 tig0018446:-168013 None:intergenic
AACAAAGCAGCAACCATAAG+AGG 0.442942 tig0018446:-167614 None:intergenic
ACCGAGCGTGCGGCTCAGAT+AGG 0.449079 tig0018446:+167690 Msa1366310:CDS
TTCTGCTAGCTCAGTTGCCA+TGG 0.451503 tig0018446:+168900 Msa1366310:CDS
AAGGTTGGATCAAAAGAAAT+GGG 0.451915 tig0018446:+167575 Msa1366310:CDS
ATCTGCTTCGCGACATCTGA+AGG 0.452178 tig0018446:-168567 None:intergenic
TCGGTTTCTAAGAGTATCTT+CGG 0.453417 tig0018446:+167845 Msa1366310:CDS
GGAAGGGATCATGCAACAAA+GGG 0.458959 tig0018446:+168197 Msa1366310:CDS
ACTCCTGCAAGCCTATTTGA+AGG 0.462316 tig0018446:-168664 None:intergenic
TTATGGTATGGAAGGAAGGT+TGG 0.462581 tig0018446:+167560 Msa1366310:CDS
TACAACGGTCGAAGCTCCTC+TGG 0.468452 tig0018446:-168340 None:intergenic
GCATGATCCCTTCCGAAACT+CGG 0.472085 tig0018446:-168188 None:intergenic
ATTGAGTTCATCGCCGCGGA+TGG 0.473676 tig0018446:-168974 None:intergenic
AGGTGAAGGTAGAGCAGAAC+CGG 0.476212 tig0018446:-168371 None:intergenic
CCCTATCTGAGCCGCACGCT+CGG 0.476697 tig0018446:-167691 None:intergenic
CATGATCCCTTCCGAAACTC+GGG 0.480869 tig0018446:-168187 None:intergenic
GTCAGGTAGGGATGGATCCA+TGG 0.481206 tig0018446:-167996 None:intergenic
TCCATTCTTTATGTCAGGTA+GGG 0.481887 tig0018446:-168008 None:intergenic
TTCTTTATGTCAGGTAGGGA+TGG 0.483088 tig0018446:-168004 None:intergenic
CCCTTCCGAAACTCGGGGCA+AGG 0.484081 tig0018446:-168181 None:intergenic
GTATCTTCGGTTTAAGGAAG+AGG 0.484626 tig0018446:+167858 Msa1366310:CDS
CGGCGCCTTGCATTCTCCCC+AGG 0.487495 tig0018446:-168127 None:intergenic
CCCTTGCCCCGAGTTTCGGA+AGG 0.487676 tig0018446:+168180 Msa1366310:CDS
GCAGCATCATCGCCTACGGC+TGG 0.487982 tig0018446:+168499 Msa1366310:CDS
CGACCGTTGTATGCTTCTAC+CGG 0.490079 tig0018446:+168352 Msa1366310:CDS
GAAAATAGGTTTCCAAATGG+TGG 0.491205 tig0018446:+167925 Msa1366310:CDS
AGCCTTGTTCTATATTGAGC+AGG 0.500362 tig0018446:-168265 None:intergenic
AGGGTTATGGTATGGAAGGA+AGG 0.505824 tig0018446:+167556 Msa1366310:CDS
TCCCTACCTGACATAAAGAA+TGG 0.512480 tig0018446:+168007 Msa1366310:CDS
AGTGCCCCTTTGTTCAACCT+GGG 0.517209 tig0018446:+168110 Msa1366310:CDS
TAAGAAAATAGGTTTCCAAA+TGG 0.517234 tig0018446:+167922 Msa1366310:CDS
CAAAGTGAAGCCTTGTTCAA+GGG 0.517860 tig0018446:+168066 Msa1366310:CDS
GCTTTGGCTCCGCTTCGAAA+AGG 0.520561 tig0018446:-168833 None:intergenic
AAGTAGGGTTATGGTATGGA+AGG 0.522426 tig0018446:+167552 Msa1366310:CDS
GTGCAGTGACTTGATTGTCT+CGG 0.522663 tig0018446:+167826 Msa1366310:CDS
GATCATGATGTTGATGAAGT+AGG 0.526063 tig0018446:+167536 Msa1366310:CDS
GGTGAATTTGAATATGATGT+AGG 0.526620 tig0018446:-168391 None:intergenic
ATGTTGATGAAGTAGGGTTA+TGG 0.527912 tig0018446:+167543 Msa1366310:CDS
CTTCGCGACATCTGAAGGGT+AGG 0.527922 tig0018446:-168562 None:intergenic
CCGGTTACCGTTTGCATCAG+CGG 0.529093 tig0018446:-167804 None:intergenic
GCTGCTTTGTTTGGAAGCAA+GGG 0.529208 tig0018446:+167626 Msa1366310:CDS
GAGCTAGCAGAAGGAAGCTC+GGG 0.529540 tig0018446:-168889 None:intergenic
ATCATGATGTTGATGAAGTA+GGG 0.530587 tig0018446:+167537 Msa1366310:CDS
TTCAACCTGGGGAGAATGCA+AGG 0.530956 tig0018446:+168122 Msa1366310:CDS
TTCACCAAGTGCTTCTTCGG+TGG 0.535677 tig0018446:+168408 Msa1366310:CDS
TGAGCTAGCAGAAGGAAGCT+CGG 0.538209 tig0018446:-168890 None:intergenic
AAGGAGATGCAAGACCTGTA+AGG 0.541933 tig0018446:-168645 None:intergenic
GTTAATTCACTTGTGGAGGA+TGG 0.542625 tig0018446:+169108 Msa1366310:CDS
AGCTTTATTGAGCGCAGCAG+CGG 0.546518 tig0018446:+168853 Msa1366310:CDS
AGGAGAGCCCCAACCAGAGA+AGG 0.547335 tig0018446:-168932 None:intergenic
CCGAGCGTGCGGCTCAGATA+GGG 0.547437 tig0018446:+167691 Msa1366310:CDS
CTCCTGATGGAAAATTAGAC+TGG 0.548612 tig0018446:+168950 Msa1366310:CDS
CGGAAGGGATCATGCAACAA+AGG 0.550435 tig0018446:+168196 Msa1366310:CDS
CACGTGTAATGATATTTCCT+CGG 0.552027 tig0018446:-168154 None:intergenic
AATGATATTTCCTCGGATCA+CGG 0.552863 tig0018446:-168147 None:intergenic
ATGGGTTAATTCACTTGTGG+AGG 0.556283 tig0018446:+169104 Msa1366310:CDS
GTTAGTTCCGCTGATGCAAA+CGG 0.558347 tig0018446:+167797 Msa1366310:CDS
CAGAAGTAGCACTTCAACAA+TGG 0.559092 tig0018446:+169032 Msa1366310:CDS
ATCTGCAGCATCATCGCCTA+CGG 0.562339 tig0018446:+168495 Msa1366310:CDS
TTGTTCTGCTGCTTCTGCCG+AGG 0.575926 tig0018446:+167742 Msa1366310:CDS
TTCCATTCTTTATGTCAGGT+AGG 0.576271 tig0018446:-168009 None:intergenic
CCTTCCGAAACTCGGGGCAA+GGG 0.578389 tig0018446:-168180 None:intergenic
AGAGAAGGCAGAACGTTCCA+TGG 0.578816 tig0018446:-168917 None:intergenic
TGAAACTAGATGCACGAGAA+GGG 0.581662 tig0018446:+168297 Msa1366310:CDS
GTGCCCCTTTGTTCAACCTG+GGG 0.583668 tig0018446:+168111 Msa1366310:CDS
GAATCCACCGAAGAAGCACT+TGG 0.583749 tig0018446:-168412 None:intergenic
TTGATTAGGGAAAATGAATG+TGG 0.584217 tig0018446:+167419 Msa1366310:CDS
TTCGCGACATCTGAAGGGTA+GGG 0.585354 tig0018446:-168561 None:intergenic
ATCATGGGTTAATTCACTTG+TGG 0.586994 tig0018446:+169101 Msa1366310:CDS
CTAATCAAACCAGTTTGACA+AGG 0.588552 tig0018446:-167404 None:intergenic
ATATAGAACAAGGCTTTGCA+AGG 0.588932 tig0018446:+168273 Msa1366310:CDS
GTGCTCTGATGCATTGATGT+CGG 0.590144 tig0018446:-168733 None:intergenic
ATGAAACTAGATGCACGAGA+AGG 0.590481 tig0018446:+168296 Msa1366310:CDS
TCAGGTAGGGATGGATCCAT+GGG 0.592467 tig0018446:-167995 None:intergenic
ATGCAAGGCGCCGTGATCCG+AGG 0.595327 tig0018446:+168137 Msa1366310:CDS
CCTTGCCCCGAGTTTCGGAA+GGG 0.595695 tig0018446:+168181 Msa1366310:CDS
TCGGTTAACATCAATGCGGC+CGG 0.598055 tig0018446:-167672 None:intergenic
GTAGGCGATGATGCTGCAGA+TGG 0.600318 tig0018446:-168493 None:intergenic
GATGAAGTAGGGTTATGGTA+TGG 0.604921 tig0018446:+167548 Msa1366310:CDS
GTGGAGCAACAACAAGATGT+AGG 0.606526 tig0018446:+167944 Msa1366310:CDS
ATTCTCCCCAGGTTGAACAA+AGG 0.606975 tig0018446:-168116 None:intergenic
GCGATGAACTCAATAAAATG+AGG 0.608002 tig0018446:+168983 Msa1366310:CDS
TTGGCTGAAAACTCAAGCAA+AGG 0.608890 tig0018446:-167473 None:intergenic
TGTGGAGGATGGTCCAACAA+TGG 0.610835 tig0018446:+169119 Msa1366310:CDS
TGAGAAGAAAGATTATCCCA+TGG 0.621054 tig0018446:+167979 Msa1366310:CDS
ATGGCAACTGAGCTAGCAGA+AGG 0.621567 tig0018446:-168898 None:intergenic
TTCCACATGGTTCCAGCCGT+AGG 0.622275 tig0018446:-168511 None:intergenic
AAGAAATGGGATATGAAGAG+AGG 0.624986 tig0018446:+167588 Msa1366310:CDS
TGAGAATAAGCCCTTGAACA+AGG 0.627841 tig0018446:-168076 None:intergenic
AAAACAAAAGTCATGTGCAA+CGG 0.630659 tig0018446:+167368 None:intergenic
AAGCAAAAGTTTGACAACCT+CGG 0.631058 tig0018446:-167759 None:intergenic
TCTGCTTCGCGACATCTGAA+GGG 0.637977 tig0018446:-168566 None:intergenic
ATTGATGTTAACCGAGCGTG+CGG 0.642447 tig0018446:+167680 Msa1366310:CDS
ATCCTGCTCAATATAGAACA+AGG 0.645051 tig0018446:+168263 Msa1366310:CDS
AGACAATCAAGTCACTGCAC+CGG 0.647436 tig0018446:-167823 None:intergenic
GATGCTGCAGATGGAGTCAA+AGG 0.650089 tig0018446:-168484 None:intergenic
TTTGAATATGATGTAGGTGA+AGG 0.654129 tig0018446:-168385 None:intergenic
AAATTAGACTGGTCCATCCG+CGG 0.660945 tig0018446:+168961 Msa1366310:CDS
CGCCTACGGCTGGAACCATG+TGG 0.662248 tig0018446:+168509 Msa1366310:CDS
GCAGATTGTATCGAAGAAGA+CGG 0.671424 tig0018446:-168451 None:intergenic
AGCACAAACCAGCAACTTTG+GGG 0.680243 tig0018446:+168751 Msa1366310:CDS
TTAAGGAAGAGGATACTACA+AGG 0.680580 tig0018446:+167869 Msa1366310:CDS
ACAAGGCAATGTTGATGACG+AGG 0.685237 tig0018446:+167886 Msa1366310:CDS
TCTCCCCAGGTTGAACAAAG+GGG 0.687449 tig0018446:-168114 None:intergenic
CTGAATCTGAGTCTTCCACA+TGG 0.688443 tig0018446:-168524 None:intergenic
ATGATCCCTTCCGAAACTCG+GGG 0.698351 tig0018446:-168186 None:intergenic
GCTGCAGATGGAGTCAAAGG+TGG 0.704806 tig0018446:-168481 None:intergenic
GAAGGGATCATGCAACAAAG+GGG 0.715610 tig0018446:+168198 Msa1366310:CDS
AGAAACAGCACAATGAAGAG+CGG 0.722067 tig0018446:-167717 None:intergenic
GATGCTTGTGAATATGCACA+TGG 0.754358 tig0018446:+168220 Msa1366310:CDS
GAACCGGTAGAAGCATACAA+CGG 0.780700 tig0018446:-168355 None:intergenic
ACGCTCGGTTAACATCAATG+CGG 0.809532 tig0018446:-167676 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TATATAGTACAGATGAATTT+AGG + tig0018446:168032-168051 Msa1366310:CDS 20.0%
! TTATCTTATATTCTTGGAAC+CGG + tig0018446:167653-167672 Msa1366310:CDS 25.0%
!! TTTGGAAACCTATTTTCTTA+AGG - tig0018446:167922-167941 None:intergenic 25.0%
! TAAGAAAATAGGTTTCCAAA+TGG + tig0018446:167922-167941 Msa1366310:CDS 25.0%
! TATATTCCATTCTTTATGTC+AGG - tig0018446:168016-168035 None:intergenic 25.0%
!!! AAAATGTTTTCAAGGTTAGT+AGG - tig0018446:168694-168713 None:intergenic 25.0%
!! ACTAACCTTGAAAACATTTT+CGG + tig0018446:168694-168713 Msa1366310:CDS 25.0%
! TTTGAATTGGTAAGATCAAA+AGG - tig0018446:168781-168800 None:intergenic 25.0%
!! ATGAGGAAAACTTATTCTTT+TGG + tig0018446:169000-169019 Msa1366310:CDS 25.0%
! TCATGGCTTAAAAATATATC+TGG - tig0018446:169081-169100 None:intergenic 25.0%
!!! TATTTTTAAGCCATGAATCA+TGG + tig0018446:169085-169104 Msa1366310:CDS 25.0%
!!! ATTTTTAAGCCATGAATCAT+GGG + tig0018446:169086-169105 Msa1366310:CDS 25.0%
AAAAGAAAACCTTGTCAAAC+TGG + tig0018446:167395-167414 Msa1366310:CDS 30.0%
!! TTGTCAAACTGGTTTGATTA+GGG + tig0018446:167406-167425 Msa1366310:CDS 30.0%
TTGATTAGGGAAAATGAATG+TGG + tig0018446:167419-167438 Msa1366310:CDS 30.0%
TTCAGCCAAAGATGATATAA+TGG + tig0018446:167487-167506 Msa1366310:CDS 30.0%
! ATCATGATGTTGATGAAGTA+GGG + tig0018446:167537-167556 Msa1366310:CDS 30.0%
AAGGTTGGATCAAAAGAAAT+GGG + tig0018446:167575-167594 Msa1366310:CDS 30.0%
GGTGTGTTATCTTATATTCT+TGG + tig0018446:167647-167666 Msa1366310:CDS 30.0%
GACGATTTCCTTAAGAAAAT+AGG + tig0018446:167911-167930 Msa1366310:CDS 30.0%
TTTGAATATGATGTAGGTGA+AGG - tig0018446:168388-168407 None:intergenic 30.0%
GGTGAATTTGAATATGATGT+AGG - tig0018446:168394-168413 None:intergenic 30.0%
! CTAACCTTGAAAACATTTTC+GGG + tig0018446:168695-168714 Msa1366310:CDS 30.0%
CTTACCAATTCAAATGTGAT+TGG + tig0018446:168787-168806 Msa1366310:CDS 30.0%
TGATCCAATCACATTTGAAT+TGG - tig0018446:168794-168813 None:intergenic 30.0%
TGTTGATCAAATTCCATTGT+TGG - tig0018446:169135-169154 None:intergenic 30.0%
CTAATCAAACCAGTTTGACA+AGG - tig0018446:167407-167426 None:intergenic 35.0%
!! CTTGTCAAACTGGTTTGATT+AGG + tig0018446:167405-167424 Msa1366310:CDS 35.0%
GAACGCCATTATATCATCTT+TGG - tig0018446:167495-167514 None:intergenic 35.0%
! GATCATGATGTTGATGAAGT+AGG + tig0018446:167536-167555 Msa1366310:CDS 35.0%
! ATGTTGATGAAGTAGGGTTA+TGG + tig0018446:167543-167562 Msa1366310:CDS 35.0%
GAAGGTTGGATCAAAAGAAA+TGG + tig0018446:167574-167593 Msa1366310:CDS 35.0%
AAGAAATGGGATATGAAGAG+AGG + tig0018446:167588-167607 Msa1366310:CDS 35.0%
AAGCAAAAGTTTGACAACCT+CGG - tig0018446:167762-167781 None:intergenic 35.0%
!! TCGGTTTCTAAGAGTATCTT+CGG + tig0018446:167845-167864 Msa1366310:CDS 35.0%
!! CTAAGAGTATCTTCGGTTTA+AGG + tig0018446:167852-167871 Msa1366310:CDS 35.0%
TTAAGGAAGAGGATACTACA+AGG + tig0018446:167869-167888 Msa1366310:CDS 35.0%
GAAAATAGGTTTCCAAATGG+TGG + tig0018446:167925-167944 Msa1366310:CDS 35.0%
TGAGAAGAAAGATTATCCCA+TGG + tig0018446:167979-167998 Msa1366310:CDS 35.0%
TCCATTCTTTATGTCAGGTA+GGG - tig0018446:168011-168030 None:intergenic 35.0%
TTCCATTCTTTATGTCAGGT+AGG - tig0018446:168012-168031 None:intergenic 35.0%
AATGATATTTCCTCGGATCA+CGG - tig0018446:168150-168169 None:intergenic 35.0%
CACGTGTAATGATATTTCCT+CGG - tig0018446:168157-168176 None:intergenic 35.0%
!! CACATGGTATTTTTGAATGC+TGG + tig0018446:168236-168255 Msa1366310:CDS 35.0%
ATCCTGCTCAATATAGAACA+AGG + tig0018446:168263-168282 Msa1366310:CDS 35.0%
ATATAGAACAAGGCTTTGCA+AGG + tig0018446:168273-168292 Msa1366310:CDS 35.0%
AAATTCACCAAGTGCTTCTT+CGG + tig0018446:168405-168424 Msa1366310:CDS 35.0%
! ATGACCCGAAAATGTTTTCA+AGG - tig0018446:168702-168721 None:intergenic 35.0%
GCGATGAACTCAATAAAATG+AGG + tig0018446:168983-169002 Msa1366310:CDS 35.0%
GTGAATTAACCCATGATTCA+TGG - tig0018446:169098-169117 None:intergenic 35.0%
! ATCATGGGTTAATTCACTTG+TGG + tig0018446:169101-169120 Msa1366310:CDS 35.0%
TTGGCTGAAAACTCAAGCAA+AGG - tig0018446:167476-167495 None:intergenic 40.0%
GATGAAGTAGGGTTATGGTA+TGG + tig0018446:167548-167567 Msa1366310:CDS 40.0%
AAGTAGGGTTATGGTATGGA+AGG + tig0018446:167552-167571 Msa1366310:CDS 40.0%
TTATGGTATGGAAGGAAGGT+TGG + tig0018446:167560-167579 Msa1366310:CDS 40.0%
AACAAAGCAGCAACCATAAG+AGG - tig0018446:167617-167636 None:intergenic 40.0%
!! CTTATGGTTGCTGCTTTGTT+TGG + tig0018446:167617-167636 Msa1366310:CDS 40.0%
AGAAACAGCACAATGAAGAG+CGG - tig0018446:167720-167739 None:intergenic 40.0%
GTATCTTCGGTTTAAGGAAG+AGG + tig0018446:167858-167877 Msa1366310:CDS 40.0%
TTCTTTATGTCAGGTAGGGA+TGG - tig0018446:168007-168026 None:intergenic 40.0%
TCCCTACCTGACATAAAGAA+TGG + tig0018446:168007-168026 Msa1366310:CDS 40.0%
!! TCAAAGTGAAGCCTTGTTCA+AGG + tig0018446:168065-168084 Msa1366310:CDS 40.0%
!! CAAAGTGAAGCCTTGTTCAA+GGG + tig0018446:168066-168085 Msa1366310:CDS 40.0%
TGAGAATAAGCCCTTGAACA+AGG - tig0018446:168079-168098 None:intergenic 40.0%
CAAGGGCTTATTCTCATGAT+TGG + tig0018446:168083-168102 Msa1366310:CDS 40.0%
GATGCTTGTGAATATGCACA+TGG + tig0018446:168220-168239 Msa1366310:CDS 40.0%
!! AGCCTTGTTCTATATTGAGC+AGG - tig0018446:168268-168287 None:intergenic 40.0%
ATGAAACTAGATGCACGAGA+AGG + tig0018446:168296-168315 Msa1366310:CDS 40.0%
TGAAACTAGATGCACGAGAA+GGG + tig0018446:168297-168316 Msa1366310:CDS 40.0%
GCAGATTGTATCGAAGAAGA+CGG - tig0018446:168454-168473 None:intergenic 40.0%
GAACTTGAAAATCGCCTTAC+AGG + tig0018446:168631-168650 Msa1366310:CDS 40.0%
GTCTTGCATCTCCTTCAAAT+AGG + tig0018446:168653-168672 Msa1366310:CDS 40.0%
CTCCTGATGGAAAATTAGAC+TGG + tig0018446:168950-168969 Msa1366310:CDS 40.0%
! GACCAGTCTAATTTTCCATC+AGG - tig0018446:168955-168974 None:intergenic 40.0%
! TTTTATTGAGTTCATCGCCG+CGG - tig0018446:168981-169000 None:intergenic 40.0%
CAGAAGTAGCACTTCAACAA+TGG + tig0018446:169032-169051 Msa1366310:CDS 40.0%
! ATGGGTTAATTCACTTGTGG+AGG + tig0018446:169104-169123 Msa1366310:CDS 40.0%
GTTAATTCACTTGTGGAGGA+TGG + tig0018446:169108-169127 Msa1366310:CDS 40.0%
AGGGTTATGGTATGGAAGGA+AGG + tig0018446:167556-167575 Msa1366310:CDS 45.0%
TGAAGAGAGGACACCTCTTA+TGG + tig0018446:167601-167620 Msa1366310:CDS 45.0%
!! TGCTGCTTTGTTTGGAAGCA+AGG + tig0018446:167625-167644 Msa1366310:CDS 45.0%
!! GCTGCTTTGTTTGGAAGCAA+GGG + tig0018446:167626-167645 Msa1366310:CDS 45.0%
ACGCTCGGTTAACATCAATG+CGG - tig0018446:167679-167698 None:intergenic 45.0%
ATTGATGTTAACCGAGCGTG+CGG + tig0018446:167680-167699 Msa1366310:CDS 45.0%
GTTAGTTCCGCTGATGCAAA+CGG + tig0018446:167797-167816 Msa1366310:CDS 45.0%
AGACAATCAAGTCACTGCAC+CGG - tig0018446:167826-167845 None:intergenic 45.0%
GTGCAGTGACTTGATTGTCT+CGG + tig0018446:167826-167845 Msa1366310:CDS 45.0%
!! ACAAGGCAATGTTGATGACG+AGG + tig0018446:167886-167905 Msa1366310:CDS 45.0%
!! CTTGTTGTTGCTCCACCATT+TGG - tig0018446:167940-167959 None:intergenic 45.0%
GTGGAGCAACAACAAGATGT+AGG + tig0018446:167944-167963 Msa1366310:CDS 45.0%
! TTCTCCCCAGGTTGAACAAA+GGG - tig0018446:168118-168137 None:intergenic 45.0%
! ATTCTCCCCAGGTTGAACAA+AGG - tig0018446:168119-168138 None:intergenic 45.0%
GGAAGGGATCATGCAACAAA+GGG + tig0018446:168197-168216 Msa1366310:CDS 45.0%
GAAGGGATCATGCAACAAAG+GGG + tig0018446:168198-168217 Msa1366310:CDS 45.0%
! GAACCGGTAGAAGCATACAA+CGG - tig0018446:168358-168377 None:intergenic 45.0%
CTGAATCTGAGTCTTCCACA+TGG - tig0018446:168527-168546 None:intergenic 45.0%
AAGGAGATGCAAGACCTGTA+AGG - tig0018446:168648-168667 None:intergenic 45.0%
TCTCCTTCAAATAGGCTTGC+AGG + tig0018446:168661-168680 Msa1366310:CDS 45.0%
ACTCCTGCAAGCCTATTTGA+AGG - tig0018446:168667-168686 None:intergenic 45.0%
! GTGCTCTGATGCATTGATGT+CGG - tig0018446:168736-168755 None:intergenic 45.0%
AGAGCACAAACCAGCAACTT+TGG + tig0018446:168749-168768 Msa1366310:CDS 45.0%
GAGCACAAACCAGCAACTTT+GGG + tig0018446:168750-168769 Msa1366310:CDS 45.0%
AGCACAAACCAGCAACTTTG+GGG + tig0018446:168751-168770 Msa1366310:CDS 45.0%
!!! GAGTTGATGCCTTTTCGAAG+CGG + tig0018446:168824-168843 Msa1366310:CDS 45.0%
TGCTGCGCTCAATAAAGCTT+TGG - tig0018446:168852-168871 None:intergenic 45.0%
! AAATTAGACTGGTCCATCCG+CGG + tig0018446:168961-168980 Msa1366310:CDS 45.0%
TCGGTTAACATCAATGCGGC+CGG - tig0018446:167675-167694 None:intergenic 50.0%
TCAGGTAGGGATGGATCCAT+GGG - tig0018446:167998-168017 None:intergenic 50.0%
AGTGCCCCTTTGTTCAACCT+GGG + tig0018446:168110-168129 Msa1366310:CDS 50.0%
! TCTCCCCAGGTTGAACAAAG+GGG - tig0018446:168117-168136 None:intergenic 50.0%
TTCAACCTGGGGAGAATGCA+AGG + tig0018446:168122-168141 Msa1366310:CDS 50.0%
ATGATCCCTTCCGAAACTCG+GGG - tig0018446:168189-168208 None:intergenic 50.0%
CATGATCCCTTCCGAAACTC+GGG - tig0018446:168190-168209 None:intergenic 50.0%
GCATGATCCCTTCCGAAACT+CGG - tig0018446:168191-168210 None:intergenic 50.0%
CGGAAGGGATCATGCAACAA+AGG + tig0018446:168196-168215 Msa1366310:CDS 50.0%
!! CTTTTTCGCTCACAAGCCAG+AGG + tig0018446:168324-168343 Msa1366310:CDS 50.0%
CGACCGTTGTATGCTTCTAC+CGG + tig0018446:168352-168371 Msa1366310:CDS 50.0%
AGGTGAAGGTAGAGCAGAAC+CGG - tig0018446:168374-168393 None:intergenic 50.0%
TTCACCAAGTGCTTCTTCGG+TGG + tig0018446:168408-168427 Msa1366310:CDS 50.0%
!! GAATCCACCGAAGAAGCACT+TGG - tig0018446:168415-168434 None:intergenic 50.0%
GATGCTGCAGATGGAGTCAA+AGG - tig0018446:168487-168506 None:intergenic 50.0%
ATCTGCAGCATCATCGCCTA+CGG + tig0018446:168495-168514 Msa1366310:CDS 50.0%
TTCGCGACATCTGAAGGGTA+GGG - tig0018446:168564-168583 None:intergenic 50.0%
TCTGCTTCGCGACATCTGAA+GGG - tig0018446:168569-168588 None:intergenic 50.0%
ATCTGCTTCGCGACATCTGA+AGG - tig0018446:168570-168589 None:intergenic 50.0%
AAGGATTGCCCCAAAGTTGC+TGG - tig0018446:168762-168781 None:intergenic 50.0%
!! AGCTTTATTGAGCGCAGCAG+CGG + tig0018446:168853-168872 Msa1366310:CDS 50.0%
TGAGCTAGCAGAAGGAAGCT+CGG - tig0018446:168893-168912 None:intergenic 50.0%
ATGGCAACTGAGCTAGCAGA+AGG - tig0018446:168901-168920 None:intergenic 50.0%
TTCTGCTAGCTCAGTTGCCA+TGG + tig0018446:168900-168919 Msa1366310:CDS 50.0%
AGAGAAGGCAGAACGTTCCA+TGG - tig0018446:168920-168939 None:intergenic 50.0%
ATGGAACGTTCTGCCTTCTC+TGG + tig0018446:168919-168938 Msa1366310:CDS 50.0%
AACGTTCTGCCTTCTCTGGT+TGG + tig0018446:168923-168942 Msa1366310:CDS 50.0%
ACGTTCTGCCTTCTCTGGTT+GGG + tig0018446:168924-168943 Msa1366310:CDS 50.0%
TGTGGAGGATGGTCCAACAA+TGG + tig0018446:169119-169138 Msa1366310:CDS 50.0%
! TTGTTCTGCTGCTTCTGCCG+AGG + tig0018446:167742-167761 Msa1366310:CDS 55.0%
CCGGTTACCGTTTGCATCAG+CGG - tig0018446:167807-167826 None:intergenic 55.0%
CCGCTGATGCAAACGGTAAC+CGG + tig0018446:167804-167823 Msa1366310:CDS 55.0%
GTCAGGTAGGGATGGATCCA+TGG - tig0018446:167999-168018 None:intergenic 55.0%
GAGTGCCCCTTTGTTCAACC+TGG + tig0018446:168109-168128 Msa1366310:CDS 55.0%
GTGCCCCTTTGTTCAACCTG+GGG + tig0018446:168111-168130 Msa1366310:CDS 55.0%
TACAACGGTCGAAGCTCCTC+TGG - tig0018446:168343-168362 None:intergenic 55.0%
GCTGCAGATGGAGTCAAAGG+TGG - tig0018446:168484-168503 None:intergenic 55.0%
GTAGGCGATGATGCTGCAGA+TGG - tig0018446:168496-168515 None:intergenic 55.0%
TTCCACATGGTTCCAGCCGT+AGG - tig0018446:168514-168533 None:intergenic 55.0%
CTTCGCGACATCTGAAGGGT+AGG - tig0018446:168565-168584 None:intergenic 55.0%
! GCTTTGGCTCCGCTTCGAAA+AGG - tig0018446:168836-168855 None:intergenic 55.0%
GAGCTAGCAGAAGGAAGCTC+GGG - tig0018446:168892-168911 None:intergenic 55.0%
CGTTCTGCCTTCTCTGGTTG+GGG + tig0018446:168925-168944 Msa1366310:CDS 55.0%
ATTGAGTTCATCGCCGCGGA+TGG - tig0018446:168977-168996 None:intergenic 55.0%
GTGTCCCTTGCCCCGAGTTT+CGG + tig0018446:168176-168195 Msa1366310:CDS 60.0%
CCTTCCGAAACTCGGGGCAA+GGG - tig0018446:168183-168202 None:intergenic 60.0%
CCTTGCCCCGAGTTTCGGAA+GGG + tig0018446:168181-168200 Msa1366310:CDS 60.0%
AGGAGAGCCCCAACCAGAGA+AGG - tig0018446:168935-168954 None:intergenic 60.0%
TCTGGTTGGGGCTCTCCTGA+TGG + tig0018446:168937-168956 Msa1366310:CDS 60.0%
ACCGAGCGTGCGGCTCAGAT+AGG + tig0018446:167690-167709 Msa1366310:CDS 65.0%
CCCTATCTGAGCCGCACGCT+CGG - tig0018446:167694-167713 None:intergenic 65.0%
CCGAGCGTGCGGCTCAGATA+GGG + tig0018446:167691-167710 Msa1366310:CDS 65.0%
!! ATGCAAGGCGCCGTGATCCG+AGG + tig0018446:168137-168156 Msa1366310:CDS 65.0%
CCCTTGCCCCGAGTTTCGGA+AGG + tig0018446:168180-168199 Msa1366310:CDS 65.0%
CCCTTCCGAAACTCGGGGCA+AGG - tig0018446:168184-168203 None:intergenic 65.0%
GCAGCATCATCGCCTACGGC+TGG + tig0018446:168499-168518 Msa1366310:CDS 65.0%
CGCCTACGGCTGGAACCATG+TGG + tig0018446:168509-168528 Msa1366310:CDS 65.0%
CGGCGCCTTGCATTCTCCCC+AGG - tig0018446:168130-168149 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
tig0018446 gene 167380 169158 167380 ID=Msa1366310;Name=Msa1366310
tig0018446 mRNA 167380 169158 167380 ID=Msa1366310-mRNA-1;Parent=Msa1366310;Name=Msa1366310-mRNA-1;_AED=0.02;_eAED=0.02;_QI=0|-1|0|1|-1|1|1|0|592
tig0018446 exon 167380 169158 167380 ID=Msa1366310-mRNA-1:exon:15025;Parent=Msa1366310-mRNA-1
tig0018446 CDS 167380 169158 167380 ID=Msa1366310-mRNA-1:cds;Parent=Msa1366310-mRNA-1
Gene Sequence

>Msa1366310

ATGTGCAACGGTTCGAAAAGAAAACCTTGTCAAACTGGTTTGATTAGGGAAAATGAATGTGGAAGACAAGAAGAACTTCATCATAAGATTTCACCTTTGCTTGAGTTTTCAGCCAAAGATGATATAATGGCGTTCACAAATGCTGTTGAAAAAGATGATCATGATGTTGATGAAGTAGGGTTATGGTATGGAAGGAAGGTTGGATCAAAAGAAATGGGATATGAAGAGAGGACACCTCTTATGGTTGCTGCTTTGTTTGGAAGCAAGGGTGTGTTATCTTATATTCTTGGAACCGGCCGCATTGATGTTAACCGAGCGTGCGGCTCAGATAGGGCTACCGCTCTTCATTGTGCTGTTTCTTGTTGTTCTGCTGCTTCTGCCGAGGTTGTCAAACTTTTGCTTGATGCATCTGCAGATGTTAGTTCCGCTGATGCAAACGGTAACCGGTGCAGTGACTTGATTGTCTCGGTTTCTAAGAGTATCTTCGGTTTAAGGAAGAGGATACTACAAGGCAATGTTGATGACGAGGACGACGATTTCCTTAAGAAAATAGGTTTCCAAATGGTGGAGCAACAACAAGATGTAGGTACATCTCGAACTGAGAAGAAAGATTATCCCATGGATCCATCCCTACCTGACATAAAGAATGGAATATATAGTACAGATGAATTTAGGATGTTTACATTCAAAGTGAAGCCTTGTTCAAGGGCTTATTCTCATGATTGGACTGAGTGCCCCTTTGTTCAACCTGGGGAGAATGCAAGGCGCCGTGATCCGAGGAAATATCATTACACGTGTGTCCCTTGCCCCGAGTTTCGGAAGGGATCATGCAACAAAGGGGATGCTTGTGAATATGCACATGGTATTTTTGAATGCTGGCTTCATCCTGCTCAATATAGAACAAGGCTTTGCAAGGATGAAACTAGATGCACGAGAAGGGTTTGCTTTTTCGCTCACAAGCCAGAGGAGCTTCGACCGTTGTATGCTTCTACCGGTTCTGCTCTACCTTCACCTACATCATATTCAAATTCACCAAGTGCTTCTTCGGTGGATTCTTTCACATTGAGCTCTCCGTCTTCTTCGATACAATCTGCGTCGACACCACCTTTGACTCCATCTGCAGCATCATCGCCTACGGCTGGAACCATGTGGAAGACTCAGATTCAGCGTAATGCTGCAGTCCCTACCCTTCAGATGTCGCGAAGCAGATTTAAAACTGCAATGAATGCTAGAAATAACGCTGAATTTCTCGAACTTGAAAATCGCCTTACAGGTCTTGCATCTCCTTCAAATAGGCTTGCAGGAGTGAATCCTACTAACCTTGAAAACATTTTCGGGTCATCAATACAATCTCCGACATCAATGCATCAGAGCACAAACCAGCAACTTTGGGGCAATCCTTTTGATCTTACCAATTCAAATGTGATTGGATCAACACAATTTAGAGTTGATGCCTTTTCGAAGCGGAGCCAAAGCTTTATTGAGCGCAGCAGCGGTGTGAGCTTTAATCCCGAGCTTCCTTCTGCTAGCTCAGTTGCCATGGAACGTTCTGCCTTCTCTGGTTGGGGCTCTCCTGATGGAAAATTAGACTGGTCCATCCGCGGCGATGAACTCAATAAAATGAGGAAAACTTATTCTTTTGGCTTTCGAAACAGAAGTAGCACTTCAACAATGGCTGCTGCATTAAGTGTTGATGATCCAGATATATTTTTAAGCCATGAATCATGGGTTAATTCACTTGTGGAGGATGGTCCAACAATGGAATTTGATCAACATTAA
Protein sequence

>Msa1366310

MCNGSKRKPCQTGLIRENECGRQEELHHKISPLLEFSAKDDIMAFTNAVEKDDHDVDEVGLWYGRKVGSKEMGYEERTPLMVAALFGSKGVLSYILGTGRIDVNRACGSDRATALHCAVSCCSAASAEVVKLLLDASADVSSADANGNRCSDLIVSVSKSIFGLRKRILQGNVDDEDDDFLKKIGFQMVEQQQDVGTSRTEKKDYPMDPSLPDIKNGIYSTDEFRMFTFKVKPCSRAYSHDWTECPFVQPGENARRRDPRKYHYTCVPCPEFRKGSCNKGDACEYAHGIFECWLHPAQYRTRLCKDETRCTRRVCFFAHKPEELRPLYASTGSALPSPTSYSNSPSASSVDSFTLSSPSSSIQSASTPPLTPSAASSPTAGTMWKTQIQRNAAVPTLQMSRSRFKTAMNARNNAEFLELENRLTGLASPSNRLAGVNPTNLENIFGSSIQSPTSMHQSTNQQLWGNPFDLTNSNVIGSTQFRVDAFSKRSQSFIERSSGVSFNPELPSASSVAMERSAFSGWGSPDGKLDWSIRGDELNKMRKTYSFGFRNRSSTSTMAAALSVDDPDIFLSHESWVNSLVEDGPTMEFDQH*