Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1375960 | AES61431.2 | 82.418 | 182 | 8 | 1 | 1 | 158 | 81 | 262 | 1.47e-100 | 301 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1375960 | G7I8T1 | 82.418 | 182 | 8 | 1 | 1 | 158 | 81 | 262 | 7.04e-101 | 301 |
| Gene ID | Type | Classification |
|---|---|---|
| Msa1375960 | TF | FAR1 |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1375960 | MtrunA17_Chr6g0475591 | 96.610 | 59 | 2 | 0 | 100 | 158 | 1 | 59 | 2.03e-36 | 121 |
| Msa1375960 | MtrunA17_Chr1g0172971 | 86.538 | 52 | 7 | 0 | 1 | 52 | 66 | 117 | 1.28e-24 | 99.4 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 26 sgRNAs with CRISPR-Local
Find 40 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCTGATGTTGGCCTGTTGTT+AGG | 0.302249 | tig0019777:-31169 | None:intergenic |
| GAGAACGTCTTATGAAGTTA+TGG | 0.394356 | tig0019777:+31434 | Msa1375960:CDS |
| GAAGTTATGGGTCAAGGAGT+TGG | 0.428685 | tig0019777:+31447 | Msa1375960:CDS |
| CGAGAAGTAAATGAAGTTCA+AGG | 0.430014 | tig0019777:+31372 | Msa1375960:CDS |
| GAAAGGGTTGGTTGTAAAGC+TGG | 0.447509 | tig0019777:+31238 | Msa1375960:CDS |
| TCTAATGACAATTCGTCAAA+AGG | 0.456711 | tig0019777:+31515 | Msa1375960:CDS |
| GCTCTAATAAATTCTAAAGC+TGG | 0.462891 | tig0019777:+31402 | Msa1375960:CDS |
| AGAACGTCTTATGAAGTTAT+GGG | 0.466325 | tig0019777:+31435 | Msa1375960:CDS |
| ACAAAATAAGAAATCGGATA+AGG | 0.469178 | tig0019777:+31087 | Msa1375960:CDS |
| GTTTGCTTAATCCTAACAAC+AGG | 0.484661 | tig0019777:+31158 | Msa1375960:CDS |
| CAATTCACAAAATAAGAAAT+CGG | 0.490263 | tig0019777:+31081 | Msa1375960:CDS |
| TTTAAGTCTGAAAATCGAAA+AGG | 0.491010 | tig0019777:-31486 | None:intergenic |
| CGTTATGTTTGTAACAAACA+AGG | 0.494754 | tig0019777:+31121 | Msa1375960:CDS |
| AGCTGGAATATCTTTGAAGC+TGG | 0.496270 | tig0019777:+31255 | Msa1375960:CDS |
| TGTCTTCCGCAGCATCAACT+TGG | 0.498648 | tig0019777:-31619 | None:intergenic |
| TTACAACCAACCCTTTCCTC+AGG | 0.505082 | tig0019777:-31232 | None:intergenic |
| TAACAAACAAGGTTTCAAGA+AGG | 0.511682 | tig0019777:+31132 | Msa1375960:CDS |
| AATTCGTCAAAAGGAGATGA+TGG | 0.518659 | tig0019777:+31524 | Msa1375960:CDS |
| AACAAACAAGGTTTCAAGAA+GGG | 0.554561 | tig0019777:+31133 | Msa1375960:CDS |
| CGTCAAAAGGAGATGATGGT+TGG | 0.572754 | tig0019777:+31528 | Msa1375960:CDS |
| GTTATGGGTCAAGGAGTTGG+AGG | 0.578191 | tig0019777:+31450 | Msa1375960:CDS |
| TCTTATGAAGTTATGGGTCA+AGG | 0.580120 | tig0019777:+31441 | Msa1375960:CDS |
| GCCAACATCAGACAGTCCAC+TGG | 0.611268 | tig0019777:+31180 | Msa1375960:CDS |
| ACCAGTGGACTGTCTGATGT+TGG | 0.614292 | tig0019777:-31181 | None:intergenic |
| TTCACAAACTCAATAACCAG+TGG | 0.633415 | tig0019777:-31196 | None:intergenic |
| TGACATCCAAGTTGATGCTG+CGG | 0.654084 | tig0019777:+31613 | Msa1375960:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | CAATTCACAAAATAAGAAAT+CGG | + | tig0019777:31081-31100 | Msa1375960:CDS | 20.0% |
| ! | ACAAAATAAGAAATCGGATA+AGG | + | tig0019777:31087-31106 | Msa1375960:CDS | 25.0% |
| ! | ATGGGTATAAGATATATAGA+TGG | + | tig0019777:31290-31309 | Msa1375960:intron | 25.0% |
| ! | TGGGTATAAGATATATAGAT+GGG | + | tig0019777:31291-31310 | Msa1375960:intron | 25.0% |
| ! | TTTAAGTCTGAAAATCGAAA+AGG | - | tig0019777:31489-31508 | None:intergenic | 25.0% |
| ! | TTGGATGTCATAGTAAAAAA+TGG | - | tig0019777:31603-31622 | None:intergenic | 25.0% |
| CGTTATGTTTGTAACAAACA+AGG | + | tig0019777:31121-31140 | Msa1375960:CDS | 30.0% | |
| TAACAAACAAGGTTTCAAGA+AGG | + | tig0019777:31132-31151 | Msa1375960:CDS | 30.0% | |
| AACAAACAAGGTTTCAAGAA+GGG | + | tig0019777:31133-31152 | Msa1375960:CDS | 30.0% | |
| TAAGATATATAGATGGGATG+TGG | + | tig0019777:31297-31316 | Msa1375960:intron | 30.0% | |
| ! | AAATGCTCTGGTTTATGTAA+TGG | - | tig0019777:31333-31352 | None:intergenic | 30.0% |
| !! | GCTCTAATAAATTCTAAAGC+TGG | + | tig0019777:31402-31421 | Msa1375960:CDS | 30.0% |
| AGAACGTCTTATGAAGTTAT+GGG | + | tig0019777:31435-31454 | Msa1375960:CDS | 30.0% | |
| TCTAATGACAATTCGTCAAA+AGG | + | tig0019777:31515-31534 | Msa1375960:CDS | 30.0% | |
| GTTTGCTTAATCCTAACAAC+AGG | + | tig0019777:31158-31177 | Msa1375960:CDS | 35.0% | |
| TTCACAAACTCAATAACCAG+TGG | - | tig0019777:31199-31218 | None:intergenic | 35.0% | |
| GAAAGTGAAAGAAAAACCTG+AGG | + | tig0019777:31216-31235 | Msa1375960:CDS | 35.0% | |
| TGAAAGAAAAACCTGAGGAA+AGG | + | tig0019777:31221-31240 | Msa1375960:CDS | 35.0% | |
| GAAAGAAAAACCTGAGGAAA+GGG | + | tig0019777:31222-31241 | Msa1375960:CDS | 35.0% | |
| !!! | AAGCTGGATGAAGTTTTGAA+TGG | + | tig0019777:31271-31290 | Msa1375960:CDS | 35.0% |
| !!! | AGCTGGATGAAGTTTTGAAT+GGG | + | tig0019777:31272-31291 | Msa1375960:CDS | 35.0% |
| !! | GTTTATGTAATGGATGACAG+TGG | - | tig0019777:31323-31342 | None:intergenic | 35.0% |
| CGAGAAGTAAATGAAGTTCA+AGG | + | tig0019777:31372-31391 | Msa1375960:CDS | 35.0% | |
| GAGAACGTCTTATGAAGTTA+TGG | + | tig0019777:31434-31453 | Msa1375960:CDS | 35.0% | |
| TCTTATGAAGTTATGGGTCA+AGG | + | tig0019777:31441-31460 | Msa1375960:CDS | 35.0% | |
| AATTCGTCAAAAGGAGATGA+TGG | + | tig0019777:31524-31543 | Msa1375960:CDS | 35.0% | |
| !! | AGCTGGAATATCTTTGAAGC+TGG | + | tig0019777:31255-31274 | Msa1375960:CDS | 40.0% |
| TTACTTCTCGAAATGCTCTC+AGG | - | tig0019777:31362-31381 | None:intergenic | 40.0% | |
| ! | TCTGATGTTGGCCTGTTGTT+AGG | - | tig0019777:31172-31191 | None:intergenic | 45.0% |
| GAAAAACCTGAGGAAAGGGT+TGG | + | tig0019777:31226-31245 | Msa1375960:CDS | 45.0% | |
| TTACAACCAACCCTTTCCTC+AGG | - | tig0019777:31235-31254 | None:intergenic | 45.0% | |
| !! | GAAAGGGTTGGTTGTAAAGC+TGG | + | tig0019777:31238-31257 | Msa1375960:CDS | 45.0% |
| CTCAGGTAACACAAATGCTC+TGG | - | tig0019777:31345-31364 | None:intergenic | 45.0% | |
| GAAGTTATGGGTCAAGGAGT+TGG | + | tig0019777:31447-31466 | Msa1375960:CDS | 45.0% | |
| ! | CGTCAAAAGGAGATGATGGT+TGG | + | tig0019777:31528-31547 | Msa1375960:CDS | 45.0% |
| !! | TGACATCCAAGTTGATGCTG+CGG | + | tig0019777:31613-31632 | Msa1375960:CDS | 45.0% |
| !! | ACCAGTGGACTGTCTGATGT+TGG | - | tig0019777:31184-31203 | None:intergenic | 50.0% |
| GTTATGGGTCAAGGAGTTGG+AGG | + | tig0019777:31450-31469 | Msa1375960:CDS | 50.0% | |
| TGTCTTCCGCAGCATCAACT+TGG | - | tig0019777:31622-31641 | None:intergenic | 50.0% | |
| GCCAACATCAGACAGTCCAC+TGG | + | tig0019777:31180-31199 | Msa1375960:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| tig0019777 | gene | 31064 | 31644 | 31064 | ID=Msa1375960;Name=Msa1375960 |
| tig0019777 | mRNA | 31064 | 31644 | 31064 | ID=Msa1375960-mRNA-1;Parent=Msa1375960;Name=Msa1375960-mRNA-1;_AED=0.18;_eAED=0.23;_QI=0|0|0|1|1|1|2|0|169 |
| tig0019777 | exon | 31064 | 31293 | 31064 | ID=Msa1375960-mRNA-1:exon:4871;Parent=Msa1375960-mRNA-1 |
| tig0019777 | exon | 31365 | 31644 | 31365 | ID=Msa1375960-mRNA-1:exon:4872;Parent=Msa1375960-mRNA-1 |
| tig0019777 | CDS | 31064 | 31293 | 31064 | ID=Msa1375960-mRNA-1:cds;Parent=Msa1375960-mRNA-1 |
| tig0019777 | CDS | 31365 | 31644 | 31365 | ID=Msa1375960-mRNA-1:cds;Parent=Msa1375960-mRNA-1 |
| Gene Sequence |
| Protein sequence |
