AlfalfaGEDB | Msa1376320

M. sativa cultivar zhongmu-4 / Msa1376320

KEGG: K04733
GO: -
PFAM: PF13947.9

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1376320	KEH44496.1	89.466	674	51	3	1	655	1	673	0.0	1216

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1376320	sp\|Q9FF31\|LRL21_ARATH	39.329	656	343	19	6	647	12	626	5.25e-142	431

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1376320	A0A072VS42	89.466	674	51	3	1	655	1	673	0.0	1216

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	bit score
Msa1376320	MtrunA17_Chr7g0251071	71.108	668	162	13	6	657	18	670	943
Msa1376320	MtrunA17_Chr7g0251061	68.533	661	182	15	7	657	20	664	868
Msa1376320	MtrunA17_Chr7g0251101	66.027	677	183	14	7	657	20	675	855
Msa1376320	MtrunA17_Chr7g0251051	67.391	644	158	13	18	654	28	626	828
Msa1376320	MtrunA17_Chr1g0212821	95.855	386	16	0	273	658	14	399	762
Msa1376320	MtrunA17_Chr7g0251001	81.963	377	66	2	284	658	1	377	638
Msa1376320	MtrunA17_Chr7g0251081	75.297	421	75	7	221	633	2	401	623
Msa1376320	MtrunA17_Chr7g0250741	73.385	387	96	4	271	654	465	847	579
Msa1376320	MtrunA17_Chr7g0250931	69.307	404	119	3	256	655	460	862	578
Msa1376320	MtrunA17_Chr4g0022331	69.010	384	117	2	258	640	449	831	562

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1376320	AT5G38260.1	39.329	656	343	19	6	647	12	626	5.33e-143	431
Msa1376320	AT1G66910.2	38.832	685	350	22	4	658	16	661	1.19e-142	431
Msa1376320	AT1G66920.2	41.167	634	313	20	32	656	33	615	4.34e-142	428
Msa1376320	AT1G66910.1	39.270	685	350	22	4	658	16	664	5.33e-142	429
Msa1376320	AT1G66920.1	40.284	633	312	20	32	656	33	607	1.01e-139	421
Msa1376320	AT1G67000.1	40.752	638	314	22	26	630	248	854	6.85e-137	423
Msa1376320	AT1G66930.2	40.852	634	317	21	32	638	252	854	3.37e-132	410
Msa1376320	AT1G66930.2	26.577	222	145	7	48	267	46	251	3.96e-13	73.6
Msa1376320	AT5G38240.2	40.382	629	307	22	20	633	27	602	1.93e-130	398
Msa1376320	AT5G38250.1	39.715	632	314	20	35	654	40	616	2.05e-129	395

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Find 138 sgRNAs with CRISPR-Local

Find 270 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAACCCGAGCTGCACAACT+TGG	0.468588	tig0019789:+131212	Msa1376320:CDS
AAACCTCAAGTATCCGTTCT+GGG	0.466254	tig0019789:+130776	Msa1376320:CDS
AAAGTAATAAACGAGGTAAA+GGG	0.389721	tig0019789:+134239	Msa1376320:CDS
AAAGTAATAGAAATGTTGGA+AGG	0.551674	tig0019789:+134962	Msa1376320:CDS
AAATCTGATATATATAGTTA+TGG	0.488656	tig0019789:+134713	Msa1376320:CDS
AAATTAGGCCAAGGAGGATA+TGG	0.353084	tig0019789:+134170	Msa1376320:CDS
AAATTGTTTGACAATCTCAG+AGG	0.704320	tig0019789:+134856	Msa1376320:CDS
AACCTCAAGTATCCGTTCTG+GGG	0.642300	tig0019789:+130777	Msa1376320:CDS
AACTCATTCCGAGATAAATT+AGG	0.407588	tig0019789:+134155	Msa1376320:CDS
AACTGATCCAACGATCCTTG+GGG	0.663564	tig0019789:-134383	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
GAGACATGATGGTATAATAT+TGG	-	tig0019789:130688-130707	None:intergenic	30.0%
TGTGAATGATGATCCTAATA+TGG	+	tig0019789:130824-130843	Msa1376320:CDS	30.0%
ATCAGTGATGCAATAAAAGA+TGG	+	tig0019789:131251-131270	Msa1376320:CDS	30.0%
ATTATTTACATACTTGAGGC+TGG	-	tig0019789:131422-131441	None:intergenic	30.0%
GGAAATAAGAACGTCAAATT+TGG	-	tig0019789:131955-131974	None:intergenic	30.0%
TACATTGCTTAAAAAAGTGG+AGG	+	tig0019789:132093-132112	Msa1376320:intron	30.0%
ACACAAATTAAGATGAAAGC+TGG	+	tig0019789:132216-132235	Msa1376320:intron	30.0%
TTACAAATTAACCCACAAAG+AGG	-	tig0019789:132264-132283	None:intergenic	30.0%
TATTGTTCTAACATATAGGG+TGG	-	tig0019789:132457-132476	None:intergenic	30.0%
GCTTGCAAAACTATTAGTTA+AGG	+	tig0019789:132513-132532	Msa1376320:intron	30.0%

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Chromosome	Type	Strat	End	Strand	Name
tig0019789	gene	130645	135111	130645	ID=Msa1376320;Name=Msa1376320
tig0019789	mRNA	130645	135111	130645	ID=Msa1376320-mRNA-1;Parent=Msa1376320;Name=Msa1376320-mRNA-1;_AED=0.21;_eAED=0.21;_QI=0\|0\|0\|1\|1\|1\|3\|0\|658
tig0019789	exon	130645	131428	130645	ID=Msa1376320-mRNA-1:exon:4997;Parent=Msa1376320-mRNA-1
tig0019789	exon	133790	133822	133790	ID=Msa1376320-mRNA-1:exon:4998;Parent=Msa1376320-mRNA-1
tig0019789	exon	133952	135111	133952	ID=Msa1376320-mRNA-1:exon:4999;Parent=Msa1376320-mRNA-1
tig0019789	CDS	130645	131428	130645	ID=Msa1376320-mRNA-1:cds;Parent=Msa1376320-mRNA-1
tig0019789	CDS	133790	133822	133790	ID=Msa1376320-mRNA-1:cds;Parent=Msa1376320-mRNA-1
tig0019789	CDS	133952	135111	133952	ID=Msa1376320-mRNA-1:cds;Parent=Msa1376320-mRNA-1

Gene Sequence

>Msa1376320

ATGAAGTTGTTTGTTCTAGCACTTTTGATGATCTCAATCACCAATATTATACCATCATGTCTCTGTGAGAATGATGATATCCAACACACCAACTGCAACGACCCTTTTATCTGCGAAAGTAGCAACATCACAAACCTCAAGTATCCGTTCTGGGGAGGAAATAGAGGAAAGTATTGCGGTGTGAATGATGATCCTAATATGGAACTCACATGTGAGGACAGTGTCCCCAAGTTAACAATGGATAATTCTTACCTGAAATACAGTATTCTTGAATGGGACAATTCAACACAGAAGCTCAGAGTTATTAGGGAAGATAGCTCGAGAGGTGATATTTGTGTTATTATCGCCAACTTCACATTCAATAGCCCTTTGTTTAAATTTTATGACGACGTCGTTAACGTGACTTTGATTTACGGTTGCCTTTCTACAACTGAAACTCTACCGAATACGTTTCACAGTGTTGACTGTATTGATAATTCATCAGTTTTGTACACCGTTGTTGATCCTGATACAGCTACTTACCCGGACTTCTGTTATAATGTGAGTACTTTCATGATTCCGATTTCAAAAACCCGAGCTGCACAACTTGGATCTGGCAATGGGACAATCAGTGATGCAATAAAAGATGGTTTTGTTTTGAAATGGACAGGAGATTATGGAGAGTGTCAAAGGTGCGTTGTTTCTGGTGGTGCCTGTGGGAACGATGGAGGAAGTGAATTCCGATGTTTCTGTAAAGATGGACCTCATACAACATCATGCAATTCAGAAAATTCTCCAGCCTCAAGTTCAAAACGGAAGAGGAAGCTTATTGTGATAGGTGTTTCAGGATCTGGAGCAACAGTATTCTTCATTATTCTTATTATTATGATTATTTGTCACCTTAAAAGGAGTACATCTGGACAACAAAGTATTTTCAGAAAGAGAAGAAAACTTGTTGACCACAATGTCGAGGTTTTCATGCAAAGTTATGAATCATCTATGCCAAGAAGATATAGTTATAGAGAAATAAAAAGGATCACAAACTCATTCCGAGATAAATTAGGCCAAGGAGGATATGGTGTTGTATACAAAGCAAGTTTACCTGATGGTCGTCCAGTGGCTGTTAAAGTAATAAACGAGGTAAAGGGGGATGGAGAAGAATTCATAAATGAAGTTGCTAGCATTAGTAGAACATCACACGTGAACATTGTGTCACTTTTAGGCTATTGTTATGAGGTGAACAAAAGAGTACTAATATATGAATTCATGCCCCAAGGATCGTTGGATCAGTTCATTTATAAAAGTGGATTTTCTGAAGCTGTTTGTGATTTTAATTGGAACACTTTGTTCCAAATTGCAATTGGCATTGCTAAAGGACTAGAGTACTTGCATCAAGGATGTAGTTCAAGGATTCTGCATCTTGATATCAAACCTCAAAACATTCTTTTGGATGAAGACTTTTGCCCAAAAATATCTGATTTTGGATTAGCAAAAATATGCCAAAGGAAGGATAGTGTTGTGTCAATACTTGGCACAAGAGGAACCATAGGATATATGGCGCCAGAAGTATATAGTAGAGCATTTGGTGGTGTTTCTTACAAATCTGATATATATAGTTATGGCATGTTGATTCTTGAAATGATTGGAGGAAGAAAGAACTATGACACTGGAGGGTCATGTACTTCAGAAATGTACTTTCCAGACTGGATTTATAAGGATCTTGAGCAAGGTAATACTCTTTCAAATTGTTTGACAATCTCAGAGGAAGAAAATGATATGGTTAGAAAGATTACTTTGGTGAGTCTATGGTGCATTCAAACGAAACCATCGGATAGACCACAGATGAATAAAGTAATAGAAATGTTGGAAGGGCCGCTTTCATTGGTGCCATATCCTCCAAAGCCCGTCCTATGTTCTCCTGAAAGGCCACCACTAGAATTGTCAGATATGTCTTCGTCCAATTTGTATGAGACGAATTCAATTACAACGTCAAACTAA

Protein sequence

>Msa1376320

MKLFVLALLMISITNIIPSCLCENDDIQHTNCNDPFICESSNITNLKYPFWGGNRGKYCGVNDDPNMELTCEDSVPKLTMDNSYLKYSILEWDNSTQKLRVIREDSSRGDICVIIANFTFNSPLFKFYDDVVNVTLIYGCLSTTETLPNTFHSVDCIDNSSVLYTVVDPDTATYPDFCYNVSTFMIPISKTRAAQLGSGNGTISDAIKDGFVLKWTGDYGECQRCVVSGGACGNDGGSEFRCFCKDGPHTTSCNSENSPASSSKRKRKLIVIGVSGSGATVFFIILIIMIICHLKRSTSGQQSIFRKRRKLVDHNVEVFMQSYESSMPRRYSYREIKRITNSFRDKLGQGGYGVVYKASLPDGRPVAVKVINEVKGDGEEFINEVASISRTSHVNIVSLLGYCYEVNKRVLIYEFMPQGSLDQFIYKSGFSEAVCDFNWNTLFQIAIGIAKGLEYLHQGCSSRILHLDIKPQNILLDEDFCPKISDFGLAKICQRKDSVVSILGTRGTIGYMAPEVYSRAFGGVSYKSDIYSYGMLILEMIGGRKNYDTGGSCTSEMYFPDWIYKDLEQGNTLSNCLTISEEENDMVRKITLVSLWCIQTKPSDRPQMNKVIEMLEGPLSLVPYPPKPVLCSPERPPLELSDMSSSNLYETNSITTSN*