Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1378420 | AES87959.1 | 73.504 | 117 | 17 | 2 | 1 | 103 | 1 | 117 | 7.65e-45 | 151 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1378420 | G7JTJ2 | 73.504 | 117 | 17 | 2 | 1 | 103 | 1 | 117 | 3.65e-45 | 151 |
| Gene ID | Type | Classification |
|---|---|---|
| Msa1378420 | TF | C2H2 |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1378420 | MtrunA17_Chr4g0019481 | 73.504 | 117 | 17 | 2 | 1 | 103 | 1 | 117 | 7.03e-49 | 151 |
| Msa1378420 | MtrunA17_Chr4g0019551 | 76.923 | 117 | 13 | 2 | 1 | 103 | 1 | 117 | 8.20e-49 | 150 |
| Msa1378420 | MtrunA17_Chr4g0019471 | 65.649 | 131 | 17 | 2 | 1 | 103 | 1 | 131 | 3.98e-46 | 144 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 18 sgRNAs with CRISPR-Local
Find 22 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCTCATTCATCTGGTGTATA+AGG | 0.184471 | tig0020061:+24932 | None:intergenic |
| TGTGGTTGAGGCTGATTATT+TGG | 0.247122 | tig0020061:+25094 | None:intergenic |
| CTCGATTCTAATATTAAATC+TGG | 0.264896 | tig0020061:+25133 | None:intergenic |
| CTCATTCATCTGGTGTATAA+GGG | 0.356164 | tig0020061:+24933 | None:intergenic |
| AGGTCGAAAGCGCGAACATT+AGG | 0.356569 | tig0020061:+24962 | None:intergenic |
| GGAGGTTGGTTCAAATCAAA+TGG | 0.432622 | tig0020061:+24983 | None:intergenic |
| CTGGTGTATAAGGGATATTA+AGG | 0.496508 | tig0020061:+24942 | None:intergenic |
| TGAGGCTGATTATTTGGTGG+TGG | 0.505165 | tig0020061:+25100 | None:intergenic |
| AAATCAAATGGTTGCACGCG+TGG | 0.532950 | tig0020061:+24995 | None:intergenic |
| AAGCGCGAACATTAGGAGGT+TGG | 0.540533 | tig0020061:+24969 | None:intergenic |
| TCGAAAGCGCGAACATTAGG+AGG | 0.542553 | tig0020061:+24965 | None:intergenic |
| ACTGCTAAAGCTTGTGGAGA+TGG | 0.545041 | tig0020061:+25178 | None:intergenic |
| AATGGTTGCACGCGTGGAGG+TGG | 0.556418 | tig0020061:+25001 | None:intergenic |
| ACATGCACTGCTAAAGCTTG+TGG | 0.565788 | tig0020061:+25172 | None:intergenic |
| GGTTGAGGCTGATTATTTGG+TGG | 0.570406 | tig0020061:+25097 | None:intergenic |
| TCAAATGGTTGCACGCGTGG+AGG | 0.626989 | tig0020061:+24998 | None:intergenic |
| GCATCAACCACAAAATGCAC+CGG | 0.637007 | tig0020061:-25062 | Msa1378420:CDS |
| ATATTAGAATCGAGTAAACA+AGG | 0.646902 | tig0020061:-25124 | Msa1378420:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | CTCGATTCTAATATTAAATC+TGG | + | tig0020061:25027-25046 | None:intergenic | 25.0% |
| ! | ATATTAGAATCGAGTAAACA+AGG | - | tig0020061:25033-25052 | Msa1378420:CDS | 25.0% |
| CTGGTGTATAAGGGATATTA+AGG | + | tig0020061:25218-25237 | None:intergenic | 35.0% | |
| TGTGGTTGAGGCTGATTATT+TGG | + | tig0020061:25066-25085 | None:intergenic | 40.0% | |
| ! | TGCTCATCATTTTGTGGTTG+AGG | + | tig0020061:25078-25097 | None:intergenic | 40.0% |
| !! | GGTTGATGCTCATCATTTTG+TGG | + | tig0020061:25084-25103 | None:intergenic | 40.0% |
| !! | GGTTGATGTTCAGCATTTTG+TGG | + | tig0020061:25126-25145 | None:intergenic | 40.0% |
| ! | GGAGGTTGGTTCAAATCAAA+TGG | + | tig0020061:25177-25196 | None:intergenic | 40.0% |
| ACTGCTAAAGCTTGTGGAGA+TGG | + | tig0020061:24982-25001 | None:intergenic | 45.0% | |
| ACATGCACTGCTAAAGCTTG+TGG | + | tig0020061:24988-25007 | None:intergenic | 45.0% | |
| ! | TGAGGCTGATTATTTGGTGG+TGG | + | tig0020061:25060-25079 | None:intergenic | 45.0% |
| GGTTGAGGCTGATTATTTGG+TGG | + | tig0020061:25063-25082 | None:intergenic | 45.0% | |
| GCATCAACCACAAAATGCAC+CGG | - | tig0020061:25095-25114 | Msa1378420:CDS | 45.0% | |
| ! | TCAGCATTTTGTGGTAGAGC+CGG | + | tig0020061:25117-25136 | None:intergenic | 45.0% |
| AAATCAAATGGTTGCACGCG+TGG | + | tig0020061:25165-25184 | None:intergenic | 45.0% | |
| AAGCGCGAACATTAGGAGGT+TGG | + | tig0020061:25191-25210 | None:intergenic | 50.0% | |
| ! | TCGAAAGCGCGAACATTAGG+AGG | + | tig0020061:25195-25214 | None:intergenic | 50.0% |
| ! | AGGTCGAAAGCGCGAACATT+AGG | + | tig0020061:25198-25217 | None:intergenic | 50.0% |
| ! | GGTAGAGCCGGTGCATTTTG+TGG | + | tig0020061:25105-25124 | None:intergenic | 55.0% |
| ! | CGTGGAGGTGGTGCATTTTG+TGG | + | tig0020061:25147-25166 | None:intergenic | 55.0% |
| TCAAATGGTTGCACGCGTGG+AGG | + | tig0020061:25162-25181 | None:intergenic | 55.0% | |
| AATGGTTGCACGCGTGGAGG+TGG | + | tig0020061:25159-25178 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| tig0020061 | gene | 24934 | 25245 | 24934 | ID=Msa1378420;Name=Msa1378420 |
| tig0020061 | mRNA | 24934 | 25245 | 24934 | ID=Msa1378420-mRNA-1;Parent=Msa1378420;Name=Msa1378420-mRNA-1;_AED=0.00;_eAED=0.00;_QI=0|-1|0|1|-1|1|1|0|103 |
| tig0020061 | exon | 24934 | 25245 | 24934 | ID=Msa1378420-mRNA-1:exon:6075;Parent=Msa1378420-mRNA-1 |
| tig0020061 | CDS | 24934 | 25245 | 24934 | ID=Msa1378420-mRNA-1:cds;Parent=Msa1378420-mRNA-1 |
| Gene Sequence |
| Protein sequence |
