AlfalfaGEDB | Msa1380920

M. sativa cultivar zhongmu-4 / Msa1380920

KEGG: K04733
GO: -
PFAM: PF07714.20

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1380920	GAU46924.1	75.936	374	55	7	6	361	1	357	0.0	550

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1380920	sp\|Q9FIU5\|CRLK1_ARATH	56.870	262	108	3	13	273	8	265	1.27e-95	294
Msa1380920	sp\|Q9FIU5\|CRLK1_ARATH	47.541	61	32	0	258	318	321	381	7.03e-13	72.8

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1380920	A0A2Z6NRM2	75.936	374	55	7	6	361	1	357	0.0	550

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1380920	MtrunA17_Chr4g0048951	89.170	277	27	1	1	277	1	274	0.0	516
Msa1380920	MtrunA17_Chr4g0048951	93.269	104	7	0	258	361	326	429	3.10e-61	202
Msa1380920	MtrunA17_Chr2g0319101	42.526	388	153	8	27	345	22	408	1.65e-90	278
Msa1380920	MtrunA17_Chr6g0455651	46.923	260	128	6	6	259	1	256	4.29e-67	217
Msa1380920	MtrunA17_Chr4g0044691	50.980	153	75	0	107	259	314	466	1.40e-46	168
Msa1380920	MtrunA17_Chr3g0108361	48.485	165	83	1	97	259	599	763	1.80e-44	164
Msa1380920	MtrunA17_Chr3g0108351	47.239	163	84	1	99	259	599	761	8.32e-43	159
Msa1380920	MtrunA17_Chr5g0432871	47.647	170	84	2	95	259	588	757	8.74e-43	159
Msa1380920	MtrunA17_Chr3g0143961	49.324	148	71	2	108	251	56	203	1.85e-42	151
Msa1380920	MtrunA17_Chr2g0283111	46.196	184	93	3	80	259	571	752	5.19e-42	156

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1380920	AT5G54590.3	56.870	262	108	3	13	273	8	265	1.29e-96	294
Msa1380920	AT5G54590.3	47.541	61	32	0	258	318	321	381	7.14e-14	72.8
Msa1380920	AT5G54590.4	56.870	262	108	3	13	273	8	265	1.29e-96	294
Msa1380920	AT5G54590.4	47.541	61	32	0	258	318	321	381	7.14e-14	72.8
Msa1380920	AT5G54590.2	56.870	262	108	3	13	273	8	265	1.29e-96	294
Msa1380920	AT5G54590.2	47.541	61	32	0	258	318	321	381	7.14e-14	72.8
Msa1380920	AT5G54590.1	58.182	220	90	2	13	231	8	226	1.60e-83	254
Msa1380920	AT5G15730.3	53.571	196	85	3	69	259	62	256	9.77e-64	209
Msa1380920	AT5G15730.2	53.571	196	85	3	69	259	62	256	9.77e-64	209
Msa1380920	AT5G15730.1	53.333	195	85	3	69	259	62	254	4.64e-63	207

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Find 81 sgRNAs with CRISPR-Local

Find 175 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAATCATGAGCCATTGAGC+TGG	0.533333	tig0020408:+138470	Msa1380920:CDS
AAACAGGACCAAATGCTCCA+TGG	0.621902	tig0020408:-137628	None:intergenic
AAAGCCTATGCATATTCCTA+TGG	0.531162	tig0020408:-137211	None:intergenic
AAAGTGAGGACAAGATTGCA+TGG	0.584798	tig0020408:+139642	Msa1380920:CDS
AACACGCCTTTCTGGTTAGA+AGG	0.374544	tig0020408:+137426	Msa1380920:CDS
AACATACTTGTAAGAATATT+TGG	0.053872	tig0020408:-137487	None:intergenic
AAGAATCTTGTGGGTTTGGT+GGG	0.494345	tig0020408:+138199	Msa1380920:CDS
AAGAATGCTGTCTCAGTATG+TGG	0.545605	tig0020408:+137459	Msa1380920:CDS
AAGTGAGGACAAGATTGCAT+GGG	0.628499	tig0020408:+139643	Msa1380920:CDS
AATCTTGTCCTCACTTTCCA+TGG	0.491517	tig0020408:-139636	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAAAAGTTGTAGAGAACAGT+TGG	-	tig0020408:138074-138093	None:intergenic	30.0%
GATGTAATCTTCCAAGTAAT+AGG	-	tig0020408:138177-138196	None:intergenic	30.0%
CTTTACATTTACATGAGCAA+CGG	+	tig0020408:138253-138272	Msa1380920:CDS	30.0%
TTTGTGTGAACTAAATGCAA+AGG	-	tig0020408:138732-138751	None:intergenic	30.0%
TAGTTCACACAAAGATGAAT+AGG	+	tig0020408:138739-138758	Msa1380920:intron	30.0%
AGTTCACACAAAGATGAATA+GGG	+	tig0020408:138740-138759	Msa1380920:intron	30.0%
CATTGTTTACTTCCTCTTAA+AGG	-	tig0020408:138912-138931	None:intergenic	30.0%
ACTAGGTATTAATGTCTTCT+CGG	-	tig0020408:138952-138971	None:intergenic	30.0%
GCATCATCTTTACAAAAACT+AGG	-	tig0020408:138969-138988	None:intergenic	30.0%
GTTCAATATGGACAAAAACA+AGG	-	tig0020408:139018-139037	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
tig0020408	gene	137171	139946	137171	ID=Msa1380920;Name=Msa1380920
tig0020408	mRNA	137171	139946	137171	ID=Msa1380920-mRNA-1;Parent=Msa1380920;Name=Msa1380920-mRNA-1;_AED=0.16;_eAED=0.16;_QI=0\|0\|0\|1\|1\|1\|6\|0\|361
tig0020408	exon	137171	137502	137171	ID=Msa1380920-mRNA-1:exon:7237;Parent=Msa1380920-mRNA-1
tig0020408	exon	137586	137739	137586	ID=Msa1380920-mRNA-1:exon:7238;Parent=Msa1380920-mRNA-1
tig0020408	exon	138172	138298	138172	ID=Msa1380920-mRNA-1:exon:7239;Parent=Msa1380920-mRNA-1
tig0020408	exon	138467	138552	138467	ID=Msa1380920-mRNA-1:exon:7240;Parent=Msa1380920-mRNA-1
tig0020408	exon	138649	138720	138649	ID=Msa1380920-mRNA-1:exon:7241;Parent=Msa1380920-mRNA-1
tig0020408	exon	139632	139946	139632	ID=Msa1380920-mRNA-1:exon:7242;Parent=Msa1380920-mRNA-1
tig0020408	CDS	137171	137502	137171	ID=Msa1380920-mRNA-1:cds;Parent=Msa1380920-mRNA-1
tig0020408	CDS	137586	137739	137586	ID=Msa1380920-mRNA-1:cds;Parent=Msa1380920-mRNA-1
tig0020408	CDS	138172	138298	138172	ID=Msa1380920-mRNA-1:cds;Parent=Msa1380920-mRNA-1
tig0020408	CDS	138467	138552	138467	ID=Msa1380920-mRNA-1:cds;Parent=Msa1380920-mRNA-1
tig0020408	CDS	138649	138720	138649	ID=Msa1380920-mRNA-1:cds;Parent=Msa1380920-mRNA-1
tig0020408	CDS	139632	139946	139632	ID=Msa1380920-mRNA-1:cds;Parent=Msa1380920-mRNA-1

Gene Sequence

>Msa1380920

ATGAATTCGCACGAGATGAGCAGAACTCTTTTAATTTTGGCCATAGGAATATGCATAGGCTTTGTGATTGGAGCTGGTTTGGTATTTTCAATTTTAGTCTTCTGCAGGCCTGGAAGGAAGCGTGTAGAGGTGGAGAAGAGCGGTCCTCTGAGAACCGAGGCCATTAATGTTCATGGCAAAGGGGCTGATTCCAGTGTAACATCATTATCAGACTCCAACGCGACTTTTGAGTCACCAAGAACATCGGAATGGAGTAACACGCCTTTCTGGTTAGAAGGACTTAGGAGGAAGAATGCTGTCTCAGTATGTGGAATTCCCAAATATTCTTACAAAGATATAGAAAAGGCTACATCTAATTTCACAACGGTCATAGGCCATGGAGCATTTGGTCCTGTTTACAAAGCAATCATGCCTACAGGCGAGGCAGTTGCGGTTAAAGTTCTCGGTGCTAATTCAAGACAAGGAGAACAAGAATTTTTAACTGAGGTCCTATTACTTGGAAGATTACATCATAAGAATCTTGTGGGTTTGGTGGGATATGCTGCAGAAAGAGGAAAGCACATGCTTCTTTACATTTACATGAGCAACGGCAGTCTTGCTTCTCATTTGTATGGCGAAAATCATGAGCCATTGAGCTGGAATTTGAGACTTAGCATAGCACTAGATGTTGCAAGGGTGATGGAATATCTACATTATTCGATCGATCCTCCTATCGTGCACCGTGACATCAAGTCTTCAAACATTCTATTAGACAAGTTTATGAAAGCCAAGGCTGCCATGGAAAGTGAGGACAAGATTGCATGGGAAGAAATTGTGGATCCGCAACTGAATGGAAACTATGATGTACATAAGCTTAATGACATGGCTTCACTTGCACTCAAATGTGTCAATGGTGTCTCCAAAATCCGACCTTCAATGAGGAACATTGTTCAAGCATTATCTCAACTTTATAAGAAGCCTAAGAGAAATTCTAGCCGAGTAGCTCCTCCTGCGTTAAATGAAGTGTCTGTTGAAGTGATGCTGCCAGAAACTCGTGACTTTTCTATTGAGTGTTCAGAGGAGCTGCGCAGATTGCACAGCCGATAA

Protein sequence

>Msa1380920

MNSHEMSRTLLILAIGICIGFVIGAGLVFSILVFCRPGRKRVEVEKSGPLRTEAINVHGKGADSSVTSLSDSNATFESPRTSEWSNTPFWLEGLRRKNAVSVCGIPKYSYKDIEKATSNFTTVIGHGAFGPVYKAIMPTGEAVAVKVLGANSRQGEQEFLTEVLLLGRLHHKNLVGLVGYAAERGKHMLLYIYMSNGSLASHLYGENHEPLSWNLRLSIALDVARVMEYLHYSIDPPIVHRDIKSSNILLDKFMKAKAAMESEDKIAWEEIVDPQLNGNYDVHKLNDMASLALKCVNGVSKIRPSMRNIVQALSQLYKKPKRNSSRVAPPALNEVSVEVMLPETRDFSIECSEELRRLHSR*