AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1382010

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1382010 MtrunA17_Chr8g0379021 98.870 177 2 0 1 177 1 177 8.81e-131 363
Msa1382010 MtrunA17_Chr2g0333981 71.856 167 47 0 1 167 1 167 3.94e-87 253
Msa1382010 MtrunA17_Chr4g0071601 67.232 177 48 2 1 167 1 177 1.69e-72 216
Msa1382010 MtrunA17_Chr4g0018951 64.596 161 49 2 1 161 1 153 4.31e-65 197
Msa1382010 MtrunA17_Chr1g0206701 42.169 166 86 2 8 166 5 167 5.12e-41 138
Msa1382010 MtrunA17_Chr1g0208561 42.529 174 88 3 8 174 5 173 1.01e-40 137
Msa1382010 MtrunA17_Chr3g0089141 42.529 174 88 3 8 174 5 173 2.10e-40 136
Msa1382010 MtrunA17_Chr1g0206991 40.964 166 88 2 8 166 5 167 4.71e-40 134
Msa1382010 MtrunA17_Chr8g0355091 41.566 166 87 2 8 166 5 167 8.21e-40 135
Msa1382010 MtrunA17_Chr3g0099421 41.040 173 88 3 8 173 30 195 1.02e-39 135
Msa1382010 MtrunA17_Chr2g0292791 41.566 166 87 2 8 166 5 167 1.47e-39 134
Msa1382010 MtrunA17_Chr1g0206721 40.964 166 88 2 8 166 5 167 4.23e-39 133
Msa1382010 MtrunA17_Chr1g0149391 40.964 166 88 2 8 166 5 167 5.09e-39 133
Msa1382010 MtrunA17_Chr2g0325971 42.604 169 87 2 8 169 5 170 8.19e-39 132
Msa1382010 MtrunA17_Chr1g0208581 40.230 174 92 3 8 174 5 173 9.48e-38 129
Msa1382010 MtrunA17_Chr3g0119901 38.365 159 94 1 7 161 4 162 4.36e-34 119
Msa1382010 MtrunA17_Chr3g0085241 38.095 168 94 2 7 167 36 200 1.08e-32 117
Msa1382010 MtrunA17_Chr5g0422421 38.037 163 97 2 8 166 5 167 1.92e-32 116
Msa1382010 MtrunA17_Chr5g0419601 36.585 164 100 2 8 167 5 168 1.94e-31 113
Msa1382010 MtrunA17_Chr2g0317451 36.747 166 95 2 8 166 5 167 2.48e-31 113
Msa1382010 MtrunA17_Chr2g0317481 36.747 166 95 2 8 166 5 167 2.59e-31 113
Msa1382010 MtrunA17_Chr3g0099411 43.200 125 61 2 8 125 5 126 8.47e-31 111
Msa1382010 MtrunA17_Chr2g0300441 36.464 181 101 3 8 177 5 182 1.89e-30 110
Msa1382010 MtrunA17_Chr1g0187361 35.838 173 97 3 8 173 5 170 1.94e-30 110
Msa1382010 MtrunA17_Chr2g0300421 44.882 127 60 2 8 127 5 128 6.70e-30 107
Msa1382010 MtrunA17_Chr1g0206691 36.601 153 87 2 21 166 1 150 8.01e-30 108
Msa1382010 MtrunA17_Chr1g0188671 37.576 165 93 2 8 165 43 204 3.99e-29 108
Msa1382010 MtrunA17_Chr5g0422941 35.583 163 101 2 8 166 5 167 1.07e-27 103
Msa1382010 MtrunA17_Chr3g0089151 53.488 86 33 1 8 86 5 90 3.72e-25 94.4
Msa1382010 MtrunA17_Chr7g0238991 36.747 166 93 3 10 166 17 179 1.38e-23 93.2
Msa1382010 MtrunA17_Chr8g0356071 38.806 134 77 4 12 142 2 133 3.86e-23 90.1
Msa1382010 MtrunA17_Chr1g0169761 38.312 154 73 4 12 158 2 140 4.36e-23 90.5
Msa1382010 MtrunA17_Chr4g0074101 39.241 158 81 5 12 157 2 156 7.03e-23 90.1
Msa1382010 MtrunA17_Chr8g0357771 39.806 103 60 1 12 112 2 104 4.43e-22 87.8
Msa1382010 MtrunA17_Chr1g0175611 40.000 130 66 4 12 131 2 129 1.77e-21 85.9
Msa1382010 MtrunA17_Chr8g0376821 37.500 128 72 3 12 131 2 129 4.28e-21 85.1
Msa1382010 MtrunA17_Chr1g0180591 35.915 142 76 4 12 140 2 141 2.17e-20 83.6
Msa1382010 MtrunA17_Chr1g0187351 53.968 63 29 0 21 83 1 63 7.71e-20 79.0
Msa1382010 MtrunA17_Chr8g0351041 35.000 160 87 4 13 160 3 157 2.15e-19 80.5
Msa1382010 MtrunA17_Chr1g0175601 44.186 86 46 1 12 97 2 85 2.95e-19 80.5
Msa1382010 MtrunA17_Chr4g0052061 46.479 71 38 0 12 82 2 72 3.87e-19 79.7
Msa1382010 MtrunA17_Chr3g0111841 52.542 59 28 0 18 76 2 60 4.96e-19 77.0
Msa1382010 MtrunA17_Chr8g0351031 35.000 160 87 4 13 160 3 157 6.68e-19 80.5
Msa1382010 MtrunA17_Chr3g0142191 40.157 127 60 3 12 123 2 127 8.99e-19 80.1
Msa1382010 MtrunA17_Chr8g0380021 36.313 179 87 9 4 177 10 166 1.50e-18 80.1
Msa1382010 MtrunA17_Chr8g0345001 34.911 169 98 5 2 161 5 170 1.82e-18 78.6
Msa1382010 MtrunA17_Chr3g0134451 54.930 71 31 1 12 81 2 72 2.26e-18 80.5
Msa1382010 MtrunA17_Chr4g0052081 41.250 80 47 0 12 91 2 81 2.37e-18 78.2
Msa1382010 MtrunA17_Chr3g0097101 37.097 124 62 4 8 127 13 124 4.31e-18 78.6
Msa1382010 MtrunA17_Chr2g0284911 34.899 149 80 5 2 148 7 140 5.25e-18 79.0
Msa1382010 MtrunA17_Chr4g0057151 34.161 161 95 4 13 163 3 162 5.42e-18 77.8
Msa1382010 MtrunA17_Chr8g0351051 50.000 68 34 0 12 79 2 69 1.25e-17 77.8
Msa1382010 MtrunA17_Chr1g0173161 60.714 56 22 0 12 67 2 57 2.18e-17 72.8
Msa1382010 MtrunA17_Chr1g0159661 46.875 64 34 0 12 75 2 65 3.05e-17 75.1
Msa1382010 MtrunA17_Chr5g0427401 47.059 68 36 0 12 79 2 69 3.13e-17 76.3
Msa1382010 MtrunA17_Chr3g0077501 51.471 68 33 0 12 79 2 69 3.65e-17 75.9
Msa1382010 MtrunA17_Chr4g0020221 35.294 153 91 3 12 162 2 148 4.14e-17 76.3
Msa1382010 MtrunA17_Chr3g0111831 50.820 61 30 0 19 79 11 71 5.31e-17 72.0
Msa1382010 MtrunA17_Chr3g0125241 45.714 70 36 1 12 79 27 96 6.46e-17 74.7
Msa1382010 MtrunA17_Chr4g0061671 34.043 141 75 5 12 150 2 126 1.48e-16 73.6
Msa1382010 MtrunA17_Chr4g0049901 32.934 167 98 3 13 168 29 192 1.54e-16 74.3
Msa1382010 MtrunA17_Chr1g0164791 48.649 74 36 1 12 83 2 75 1.86e-16 74.3
Msa1382010 MtrunA17_Chr4g0061691 30.000 170 108 5 12 173 2 168 1.99e-16 73.9
Msa1382010 MtrunA17_Chr5g0425191 60.377 53 21 0 12 64 2 54 2.06e-16 70.9
Msa1382010 MtrunA17_Chr7g0220111 36.364 143 70 6 12 150 2 127 2.79e-16 73.9
Msa1382010 MtrunA17_Chr7g0246381 50.000 62 31 0 12 73 2 63 3.63e-16 69.3
Msa1382010 MtrunA17_Chr6g0455511 42.708 96 50 3 12 103 2 96 3.98e-16 73.6
Msa1382010 MtrunA17_Chr4g0044071 54.386 57 26 0 12 68 2 58 4.24e-16 70.5
Msa1382010 MtrunA17_Chr6g0474371 31.515 165 92 6 12 167 2 154 5.76e-16 72.0
Msa1382010 MtrunA17_Chr2g0308251 55.556 54 24 0 12 65 2 55 5.92e-16 69.3
Msa1382010 MtrunA17_Chr5g0404951 50.000 70 33 1 12 79 2 71 6.51e-16 72.8
Msa1382010 MtrunA17_Chr5g0412271 50.000 68 34 0 12 79 2 69 7.42e-16 72.4
Msa1382010 MtrunA17_Chr5g0412461 50.000 68 34 0 12 79 2 69 7.70e-16 72.4
Msa1382010 MtrunA17_Chr2g0279821 45.588 68 37 0 12 79 2 69 1.20e-15 72.0
Msa1382010 MtrunA17_Chr1g0204111 29.825 171 96 6 7 167 13 169 1.52e-15 72.0
Msa1382010 MtrunA17_Chr8g0377561 53.704 54 25 0 12 65 2 55 1.80e-15 68.6
Msa1382010 MtrunA17_Chr5g0420091 40.816 98 50 3 12 103 2 97 1.93e-15 71.2
Msa1382010 MtrunA17_Chr7g0246431 46.479 71 38 0 13 83 3 73 2.36e-15 70.9
Msa1382010 MtrunA17_Chr5g0427921 48.276 58 30 0 12 69 2 59 3.02e-15 66.6
Msa1382010 MtrunA17_Chr3g0122251 55.357 56 25 0 12 67 2 57 4.88e-15 70.5
Msa1382010 MtrunA17_Chr4g0049541 44.118 68 38 0 12 79 2 69 1.60e-14 68.9
Msa1382010 MtrunA17_Chr5g0411691 42.647 68 39 0 12 79 2 69 1.62e-14 68.9
Msa1382010 MtrunA17_Chr8g0366231 54.717 53 24 0 12 64 2 54 1.89e-14 68.9
Msa1382010 MtrunA17_Chr2g0302091 32.558 172 96 7 10 170 35 197 4.77e-14 67.0
Msa1382010 MtrunA17_Chr7g0220121 44.928 69 37 1 12 79 2 70 4.95e-14 67.8
Msa1382010 MtrunA17_Chr3g0111931 44.286 70 32 1 24 86 2 71 6.01e-14 64.7
Msa1382010 MtrunA17_Chr5g0420081 52.830 53 25 0 12 64 2 54 6.69e-14 67.4
Msa1382010 MtrunA17_Chr3g0093131 31.159 138 90 2 29 161 36 173 8.36e-14 66.2
Msa1382010 MtrunA17_Chr4g0060841 51.923 52 25 0 12 63 2 53 5.34e-13 65.9
Msa1382010 MtrunA17_Chr1g0166611 33.981 103 51 1 26 128 20 105 1.17e-12 62.0
Msa1382010 MtrunA17_Chr5g0417661 48.077 52 27 0 12 63 57 108 1.66e-12 61.6
Msa1382010 MtrunA17_Chr1g0212151 30.380 158 87 5 12 161 2 144 2.14e-12 63.9
Msa1382010 MtrunA17_Chr4g0018421 38.356 73 44 1 12 83 2 74 2.26e-12 62.8
Msa1382010 MtrunA17_Chr6g0457851 29.252 147 84 4 12 150 12 146 8.18e-12 62.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1382010 AT5G60440.1 41.509 159 86 3 8 159 3 161 2.23e-34 123
Msa1382010 AT2G24840.1 40.506 158 89 2 10 162 61 218 1.12e-32 117
Msa1382010 AT1G65360.1 42.073 164 86 3 8 165 3 163 5.50e-32 115
Msa1382010 AT1G01530.1 38.182 165 99 2 6 167 1 165 2.57e-29 108
Msa1382010 AT4G36590.1 35.119 168 99 3 12 169 7 174 7.06e-28 105
Msa1382010 AT2G34440.1 37.838 148 84 4 12 154 2 146 2.79e-25 96.3
Msa1382010 AT1G28460.1 34.731 167 98 4 8 165 4 168 1.63e-21 86.7
Msa1382010 AT3G66656.1 46.875 96 50 1 12 107 2 96 2.59e-21 86.3
Msa1382010 AT5G51870.1 33.523 176 100 4 13 171 3 178 3.91e-21 86.3
Msa1382010 AT5G51870.5 34.932 146 83 2 5 150 18 151 1.60e-20 84.3
Msa1382010 AT5G51870.4 34.194 155 95 2 13 160 3 157 2.86e-20 84.3
Msa1382010 AT5G51870.3 34.194 155 95 2 13 160 13 167 3.15e-20 84.3
Msa1382010 AT5G51870.2 35.507 138 77 2 13 150 3 128 5.99e-20 82.4
Msa1382010 AT2G42830.2 37.063 143 69 5 9 147 14 139 7.41e-20 84.0
Msa1382010 AT2G42830.1 37.063 143 69 5 9 147 14 139 8.12e-20 83.6
Msa1382010 AT1G72350.1 32.026 153 75 3 12 164 43 166 1.82e-19 82.4
Msa1382010 AT5G10140.2 36.913 149 75 5 12 159 2 132 1.87e-19 80.9
Msa1382010 AT1G77950.3 35.099 151 87 4 12 161 2 142 2.56e-19 82.4
Msa1382010 AT1G77950.2 35.099 151 87 4 12 161 2 142 2.56e-19 82.4
Msa1382010 AT1G77950.1 35.099 151 87 4 12 161 2 142 2.56e-19 82.4
Msa1382010 AT1G77950.4 35.099 151 87 4 12 161 2 142 2.56e-19 82.4
Msa1382010 AT4G09960.6 36.129 155 85 5 12 162 3 147 2.77e-19 82.0
Msa1382010 AT4G09960.1 36.129 155 85 5 12 162 2 146 2.86e-19 82.0
Msa1382010 AT4G09960.5 36.129 155 85 5 12 162 12 156 3.44e-19 82.0
Msa1382010 AT4G09960.2 36.842 152 81 6 12 160 2 141 3.87e-19 81.3
Msa1382010 AT5G10140.4 36.913 149 75 5 12 159 2 132 3.99e-19 80.5
Msa1382010 AT5G10140.1 36.913 149 75 5 12 159 2 132 4.29e-19 80.9
Msa1382010 AT4G09960.3 36.129 155 85 5 12 162 28 172 4.73e-19 82.0
Msa1382010 AT5G10140.3 36.913 149 75 5 12 159 2 132 7.15e-19 80.1
Msa1382010 AT4G18960.1 38.776 147 65 8 6 147 14 140 8.21e-19 81.3
Msa1382010 AT1G28450.1 32.738 168 99 5 8 163 4 169 1.19e-18 79.3
Msa1382010 AT3G54340.1 57.377 61 26 0 12 72 2 62 1.66e-18 80.1
Msa1382010 AT1G29962.1 30.769 169 105 4 8 165 4 171 2.54e-18 78.6
Msa1382010 AT2G45660.2 33.540 161 89 4 13 161 3 157 2.55e-18 77.8
Msa1382010 AT4G09960.4 41.739 115 55 4 12 124 97 201 2.76e-18 80.9
Msa1382010 AT3G58780.1 39.167 120 57 4 9 124 14 121 5.20e-18 79.0
Msa1382010 AT3G58780.5 39.167 120 57 4 9 124 20 127 5.57e-18 79.0
Msa1382010 AT1G77980.1 50.704 71 34 1 12 81 2 72 7.24e-18 79.7
Msa1382010 AT3G58780.4 35.417 144 72 5 9 148 14 140 7.94e-18 79.0
Msa1382010 AT5G23260.3 32.051 156 91 3 12 163 2 146 1.04e-17 77.8
Msa1382010 AT5G23260.4 49.351 77 37 1 7 81 13 89 1.26e-17 78.2
Msa1382010 AT5G23260.1 49.351 77 37 1 7 81 13 89 1.26e-17 78.2
Msa1382010 AT2G22540.3 31.325 166 100 3 12 167 2 163 2.25e-17 77.0
Msa1382010 AT2G22540.1 31.325 166 100 3 12 167 2 163 2.25e-17 77.0
Msa1382010 AT1G22130.1 50.704 71 34 1 12 81 2 72 2.28e-17 78.6
Msa1382010 AT3G57390.3 52.941 68 32 0 12 79 2 69 2.65e-17 76.6
Msa1382010 AT5G13790.1 52.857 70 33 0 12 81 2 71 2.95e-17 77.4
Msa1382010 AT5G13790.2 52.857 70 33 0 12 81 2 71 3.19e-17 77.4
Msa1382010 AT5G62165.17 31.613 155 100 2 13 161 3 157 3.57e-17 74.7
Msa1382010 AT5G62165.12 31.613 155 100 2 13 161 3 157 3.57e-17 74.7
Msa1382010 AT5G62165.11 31.613 155 100 2 13 161 3 157 3.57e-17 74.7
Msa1382010 AT4G11880.2 31.613 155 100 2 13 161 3 157 4.30e-17 75.1
Msa1382010 AT4G11880.3 31.613 155 100 2 13 161 3 157 4.30e-17 75.1
Msa1382010 AT2G45660.1 33.750 160 88 4 13 160 3 156 4.32e-17 75.9
Msa1382010 AT3G57390.1 52.941 68 32 0 12 79 2 69 5.66e-17 76.3
Msa1382010 AT5G23260.2 51.389 72 33 1 12 81 2 73 6.78e-17 75.9
Msa1382010 AT5G15800.1 36.364 143 71 5 12 150 2 128 7.49e-17 75.9
Msa1382010 AT3G57230.2 45.588 68 37 0 12 79 2 69 8.27e-17 73.6
Msa1382010 AT4G37940.4 44.595 74 41 0 12 85 2 75 9.15e-17 75.1
Msa1382010 AT5G62165.14 44.872 78 43 0 13 90 3 80 9.29e-17 72.4
Msa1382010 AT4G37940.3 44.595 74 41 0 12 85 2 75 9.91e-17 73.9
Msa1382010 AT4G37940.1 44.595 74 41 0 12 85 2 75 9.98e-17 75.1
Msa1382010 AT4G22950.2 30.464 151 100 2 13 158 3 153 1.02e-16 73.9
Msa1382010 AT3G04100.1 26.415 159 109 2 8 158 10 168 1.06e-16 74.7
Msa1382010 AT5G51860.2 45.570 79 43 0 13 91 3 81 1.22e-16 74.3
Msa1382010 AT4G24540.1 51.471 68 33 0 12 79 2 69 1.23e-16 74.7
Msa1382010 AT3G02310.2 36.364 143 71 5 12 150 2 128 1.29e-16 74.3
Msa1382010 AT5G15800.2 36.364 143 71 5 12 150 2 128 1.35e-16 75.5
Msa1382010 AT5G62165.6 38.532 109 65 1 13 119 3 111 1.83e-16 72.8
Msa1382010 AT5G62165.9 38.532 109 65 1 13 119 3 111 1.83e-16 72.8
Msa1382010 AT5G62165.7 38.532 109 65 1 13 119 3 111 1.83e-16 72.8
Msa1382010 AT5G62165.8 38.532 109 65 1 13 119 3 111 1.83e-16 72.8
Msa1382010 AT5G62165.13 38.532 109 65 1 13 119 3 111 1.83e-16 72.8
Msa1382010 AT5G51860.1 45.570 79 43 0 13 91 3 81 1.88e-16 73.9
Msa1382010 AT5G62165.3 31.818 154 99 2 13 160 3 156 2.42e-16 73.9
Msa1382010 AT5G62165.1 31.818 154 99 2 13 160 3 156 2.42e-16 73.9
Msa1382010 AT5G62165.2 31.818 154 99 2 13 160 3 156 2.42e-16 73.9
Msa1382010 AT2G22540.2 31.515 165 96 4 12 167 2 158 3.47e-16 73.9
Msa1382010 AT5G62165.10 44.872 78 43 0 13 90 3 80 3.68e-16 73.2
Msa1382010 AT5G62165.4 44.872 78 43 0 13 90 3 80 3.68e-16 73.2
Msa1382010 AT4G22950.3 30.464 151 100 2 13 158 3 153 4.05e-16 73.6
Msa1382010 AT4G22950.1 30.464 151 100 2 13 158 3 153 4.05e-16 73.6
Msa1382010 AT3G58780.2 38.136 118 54 4 9 124 14 114 4.56e-16 73.6
Msa1382010 AT3G57230.1 45.588 68 37 0 12 79 2 69 4.59e-16 73.6
Msa1382010 AT3G57230.4 45.588 68 37 0 12 79 2 69 4.59e-16 73.6
Msa1382010 AT2G45650.1 40.816 98 36 1 12 109 2 77 5.19e-16 73.6
Msa1382010 AT4G11880.4 33.835 133 82 2 13 139 3 135 6.27e-16 72.8
Msa1382010 AT4G11880.1 33.835 133 82 2 13 139 3 135 6.27e-16 72.8
Msa1382010 AT3G02310.1 36.364 143 71 5 12 150 2 128 1.08e-15 72.8
Msa1382010 AT3G58780.3 53.333 60 28 0 9 68 14 73 1.15e-15 73.2
Msa1382010 AT5G60910.1 30.409 171 107 6 12 173 2 169 2.14e-15 72.0
Msa1382010 AT5G65070.4 44.928 69 37 1 12 79 2 70 2.61e-15 69.7
Msa1382010 AT1G69120.2 29.412 170 109 6 12 173 2 168 2.80e-15 71.2
Msa1382010 AT1G69120.1 29.412 170 109 6 12 173 2 168 3.27e-15 71.6
Msa1382010 AT2G03710.3 28.662 157 103 5 12 160 2 157 3.75e-15 70.1
Msa1382010 AT3G61120.1 44.118 68 38 0 12 79 2 69 5.81e-15 70.9
Msa1382010 AT1G24260.3 53.571 56 26 0 12 67 2 57 5.93e-15 70.5
Msa1382010 AT3G30260.1 44.928 69 37 1 12 79 2 70 6.26e-15 70.9
Msa1382010 AT1G24260.2 53.571 56 26 0 12 67 2 57 6.35e-15 70.9
Msa1382010 AT1G24260.1 53.571 56 26 0 12 67 2 57 6.46e-15 70.5
Msa1382010 AT5G65070.1 44.928 69 37 1 12 79 2 70 6.59e-15 69.7
Msa1382010 AT2G03710.2 53.571 56 26 0 12 67 2 57 9.84e-15 70.1
Msa1382010 AT2G03710.1 53.571 56 26 0 12 67 2 57 9.95e-15 70.1
Msa1382010 AT5G65060.3 44.928 69 37 1 12 79 2 70 1.02e-14 68.9
Msa1382010 AT5G65060.1 44.928 69 37 1 12 79 2 70 1.26e-14 68.9
Msa1382010 AT5G65060.2 36.036 111 66 3 12 117 2 112 1.36e-14 68.6
Msa1382010 AT5G20240.2 50.000 62 31 0 12 73 2 63 1.39e-14 67.8
Msa1382010 AT5G65050.2 44.928 69 37 1 12 79 2 70 1.57e-14 68.2
Msa1382010 AT5G65050.5 33.784 148 78 5 12 139 2 149 1.67e-14 68.6
Msa1382010 AT5G20240.1 33.566 143 86 5 12 148 2 141 1.69e-14 68.9
Msa1382010 AT2G14210.1 50.877 57 28 0 12 68 2 58 1.91e-14 69.3
Msa1382010 AT5G65070.5 44.928 69 37 1 12 79 2 70 2.01e-14 68.6
Msa1382010 AT5G65070.2 44.928 69 37 1 12 79 2 70 2.05e-14 68.9
Msa1382010 AT1G47760.1 49.153 59 30 0 12 70 2 60 2.66e-14 67.8
Msa1382010 AT1G26310.1 46.377 69 36 1 12 79 2 70 2.82e-14 68.9
Msa1382010 AT2G14210.2 50.877 57 28 0 12 68 2 58 2.88e-14 68.6
Msa1382010 AT5G65050.3 44.928 69 37 1 12 79 2 70 3.75e-14 67.8
Msa1382010 AT5G65050.1 44.928 69 37 1 12 79 2 70 3.85e-14 67.4
Msa1382010 AT5G65070.3 49.123 57 29 0 12 68 2 58 6.47e-14 67.8
Msa1382010 AT1G71692.1 51.923 52 25 0 13 64 3 54 8.83e-14 67.0
Msa1382010 AT2G22630.1 41.892 74 43 0 12 85 2 75 1.04e-13 67.0
Msa1382010 AT2G22630.2 41.892 74 43 0 12 85 2 75 1.04e-13 67.0
Msa1382010 AT5G49420.1 29.341 167 88 7 8 167 3 146 3.46e-13 66.6
Msa1382010 AT1G18750.4 46.429 56 30 0 12 67 2 57 1.13e-12 65.5
Msa1382010 AT1G18750.3 46.429 56 30 0 12 67 2 57 1.27e-12 65.5
Msa1382010 AT1G18750.1 46.429 56 30 0 12 67 2 57 1.32e-12 65.1
Msa1382010 AT4G37435.1 48.214 56 29 0 12 67 2 57 1.58e-12 63.2
Msa1382010 AT1G69540.1 39.130 69 41 1 12 79 2 70 2.14e-12 64.3
Msa1382010 AT1G69540.2 39.130 69 41 1 12 79 2 70 2.95e-12 64.3
Msa1382010 AT5G49490.1 37.209 86 48 2 3 82 34 119 3.72e-12 63.5
Msa1382010 AT5G65080.3 48.148 54 28 0 12 65 9 62 6.88e-12 61.6
Msa1382010 AT2G03060.3 48.276 58 29 1 12 68 2 59 7.57e-12 63.2
Msa1382010 AT2G03060.7 48.276 58 29 1 12 68 2 59 7.57e-12 63.2
Msa1382010 AT2G03060.1 48.276 58 29 1 12 68 2 59 7.57e-12 63.2
Msa1382010 AT5G65080.2 48.148 54 28 0 12 65 9 62 8.30e-12 61.6
Msa1382010 AT2G03060.2 48.276 58 29 1 12 68 2 59 9.17e-12 62.8
Msa1382010 AT5G38620.1 28.395 162 88 6 13 167 6 146 2.02e-11 61.6
Msa1382010 AT1G77080.11 35.849 106 47 1 12 117 2 86 7.39e-11 58.5
Msa1382010 AT1G46408.1 38.356 73 39 2 13 80 5 76 8.45e-11 59.3
Msa1382010 AT1G77080.5 35.849 106 47 1 12 117 2 86 9.30e-11 58.2

Find 31 sgRNAs with CRISPR-Local

Find 34 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTTGACTAAGATTTGGAATA+TGG 0.116779 tig0020467:-27026 Msa1382010:CDS
TGATCAGCTTGAAGAATTTA+TGG 0.178411 tig0020467:-26966 Msa1382010:CDS
TTCCATTGATGTAGCTATTA+AGG 0.197208 tig0020467:+27173 None:intergenic
AGATTTGGAATATGGGTAAT+TGG 0.238134 tig0020467:-27018 Msa1382010:CDS
CTCAAATTCCACCATGAATA+TGG 0.248872 tig0020467:-27410 None:intergenic
TTGAAAATTTGACTAAGATT+TGG 0.254246 tig0020467:-27033 Msa1382010:CDS
TTGTTTATTGTACTCTTTGT+AGG 0.257540 tig0020467:+27108 None:intergenic
TGATGTAGCTATTAAGGATT+TGG 0.289298 tig0020467:+27179 None:intergenic
TCTTAGGTTATCCATATTCA+TGG 0.338275 tig0020467:+27399 None:intergenic
TTGACTAAGATTTGGAATAT+GGG 0.365430 tig0020467:-27025 Msa1382010:CDS
TTAAGGATTTGGTCAGTGTT+TGG 0.380076 tig0020467:+27190 None:intergenic
ACCGAAACAGTGTAGTTTGC+CGG 0.381099 tig0020467:+27216 None:intergenic
TGAGAAAGTGACTTGTTTGT+TGG 0.387841 tig0020467:+27324 None:intergenic
TTTGATGTTTCAAATTCAAC+AGG 0.390139 tig0020467:-27142 Msa1382010:CDS
CTTCCTCTTCAACTCAGAAA+TGG 0.428773 tig0020467:+26940 None:intergenic
GCCGGCAAACTACACTGTTT+CGG 0.438295 tig0020467:-27217 Msa1382010:CDS
ATTTGAAACATCAAACTCAA+TGG 0.455608 tig0020467:+27150 None:intergenic
ATCCTTAATAGCTACATCAA+TGG 0.463621 tig0020467:-27175 Msa1382010:CDS
ACAATATGAGGAGGCACTAA+AGG 0.492528 tig0020467:-27089 Msa1382010:CDS
AGAGTACAATAAACAATATG+AGG 0.506442 tig0020467:-27101 Msa1382010:CDS
GGCACTAAAGGTGTTGGAGA+TGG 0.510619 tig0020467:-27077 Msa1382010:CDS
TGAGGAGGCACTAAAGGTGT+TGG 0.525149 tig0020467:-27083 Msa1382010:CDS
GAAGAGGAAGCTACTTGAAA+GGG 0.526324 tig0020467:-26927 Msa1382010:CDS
TTTGGAATATGGGTAATTGG+TGG 0.532497 tig0020467:-27015 Msa1382010:CDS
GGCAGGAGAAAACACGATGA+TGG 0.556314 tig0020467:+27237 None:intergenic
TGAAGAGGAAGCTACTTGAA+AGG 0.570414 tig0020467:-26928 Msa1382010:CDS
AGTCCATTTCTGAGTTGAAG+AGG 0.572765 tig0020467:-26943 Msa1382010:CDS
AAACAGTGTAGTTTGCCGGC+AGG 0.581035 tig0020467:+27220 None:intergenic
ACTTTCTCAAAGAGAAGACA+AGG 0.611669 tig0020467:-27310 Msa1382010:CDS
CTACAACATAGAAAACATCA+TGG 0.627330 tig0020467:+26886 None:intergenic
GTACAATAAACAATATGAGG+AGG 0.656498 tig0020467:-27098 Msa1382010:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTGAAAATTTGACTAAGATT+TGG - tig0020467:27250-27269 Msa1382010:CDS 20.0%
!! ATTTGAAACATCAAACTCAA+TGG + tig0020467:27136-27155 None:intergenic 25.0%
! TTTGATGTTTCAAATTCAAC+AGG - tig0020467:27141-27160 Msa1382010:CDS 25.0%
! TTGTTTATTGTACTCTTTGT+AGG + tig0020467:27178-27197 None:intergenic 25.0%
! AGAGTACAATAAACAATATG+AGG - tig0020467:27182-27201 Msa1382010:CDS 25.0%
!! TTTGACTAAGATTTGGAATA+TGG - tig0020467:27257-27276 Msa1382010:CDS 25.0%
!! TTGACTAAGATTTGGAATAT+GGG - tig0020467:27258-27277 Msa1382010:CDS 25.0%
AACCTAAGAAAGAAAAACAC+AGG - tig0020467:26898-26917 Msa1382010:CDS 30.0%
ACCTAAGAAAGAAAAACACA+GGG - tig0020467:26899-26918 Msa1382010:CDS 30.0%
TGATGTAGCTATTAAGGATT+TGG + tig0020467:27107-27126 None:intergenic 30.0%
ATCCTTAATAGCTACATCAA+TGG - tig0020467:27108-27127 Msa1382010:CDS 30.0%
TTCCATTGATGTAGCTATTA+AGG + tig0020467:27113-27132 None:intergenic 30.0%
GTACAATAAACAATATGAGG+AGG - tig0020467:27185-27204 Msa1382010:CDS 30.0%
! AGATTTGGAATATGGGTAAT+TGG - tig0020467:27265-27284 Msa1382010:CDS 30.0%
TGATCAGCTTGAAGAATTTA+TGG - tig0020467:27317-27336 Msa1382010:CDS 30.0%
CTACAACATAGAAAACATCA+TGG + tig0020467:27400-27419 None:intergenic 30.0%
!! TGAGAAAGTGACTTGTTTGT+TGG + tig0020467:26962-26981 None:intergenic 35.0%
ACTTTCTCAAAGAGAAGACA+AGG - tig0020467:26973-26992 Msa1382010:CDS 35.0%
! TTAAGGATTTGGTCAGTGTT+TGG + tig0020467:27096-27115 None:intergenic 35.0%
! TTTGGAATATGGGTAATTGG+TGG - tig0020467:27268-27287 Msa1382010:CDS 35.0%
! GCCCTGTGTTTTTCTTTCTT+AGG + tig0020467:26903-26922 None:intergenic 40.0%
!! TGCAAAGTTCACTCGCTTTT+CGG + tig0020467:27005-27024 None:intergenic 40.0%
!! ACAATATGAGGAGGCACTAA+AGG - tig0020467:27194-27213 Msa1382010:CDS 40.0%
! AGTCCATTTCTGAGTTGAAG+AGG - tig0020467:27340-27359 Msa1382010:CDS 40.0%
CTTCCTCTTCAACTCAGAAA+TGG + tig0020467:27346-27365 None:intergenic 40.0%
TGAAGAGGAAGCTACTTGAA+AGG - tig0020467:27355-27374 Msa1382010:CDS 40.0%
GAAGAGGAAGCTACTTGAAA+GGG - tig0020467:27356-27375 Msa1382010:CDS 40.0%
! ATCATCGTGTTTTCTCCTGC+CGG - tig0020467:27048-27067 Msa1382010:CDS 45.0%
! ACCGAAACAGTGTAGTTTGC+CGG + tig0020467:27070-27089 None:intergenic 45.0%
GGCAGGAGAAAACACGATGA+TGG + tig0020467:27049-27068 None:intergenic 50.0%
! AAACAGTGTAGTTTGCCGGC+AGG + tig0020467:27066-27085 None:intergenic 50.0%
GCCGGCAAACTACACTGTTT+CGG - tig0020467:27066-27085 Msa1382010:CDS 50.0%
!! TGAGGAGGCACTAAAGGTGT+TGG - tig0020467:27200-27219 Msa1382010:CDS 50.0%
!! GGCACTAAAGGTGTTGGAGA+TGG - tig0020467:27206-27225 Msa1382010:CDS 50.0%
Chromosome Type Strat End Strand Name
tig0020467 gene 26886 27419 26886 ID=Msa1382010;Name=Msa1382010
tig0020467 mRNA 26886 27419 26886 ID=Msa1382010-mRNA-1;Parent=Msa1382010;Name=Msa1382010-mRNA-1;_AED=0.00;_eAED=0.00;_QI=0|-1|0|1|-1|1|1|0|177
tig0020467 exon 26886 27419 26886 ID=Msa1382010-mRNA-1:exon:7901;Parent=Msa1382010-mRNA-1
tig0020467 CDS 26886 27419 26886 ID=Msa1382010-mRNA-1:cds;Parent=Msa1382010-mRNA-1
Gene Sequence

>Msa1382010

ATGAATATGGATAACCTAAGAAAGAAAAACACAGGGCGTAGAAAGATTGAGATCAAAAAGCTTGAAAAAGAAACCAACAAACAAGTCACTTTCTCAAAGAGAAGACAAGGTTTATTCCGAAAAGCGAGTGAACTTTGCACTTTATGTGATGTTCATGCAGCCATCATCGTGTTTTCTCCTGCCGGCAAACTACACTGTTTCGGTGAACCAAACACTGACCAAATCCTTAATAGCTACATCAATGGAACCATTGAGTTTGATGTTTCAAATTCAACAGGAAATTCTTCAACCTACAAAGAGTACAATAAACAATATGAGGAGGCACTAAAGGTGTTGGAGATGGAAAAACAGAAACTTGCTGATGTTGAAAATTTGACTAAGATTTGGAATATGGGTAATTGGTGGAATGAATCTATTGATGAGATGAATAGTGATCAGCTTGAAGAATTTATGGAGTCCATTTCTGAGTTGAAGAGGAAGCTACTTGAAAGGGCTGATGAACATACGAATACCATGATGTTTTCTATGTTGTAG
Protein sequence

>Msa1382010

MNMDNLRKKNTGRRKIEIKKLEKETNKQVTFSKRRQGLFRKASELCTLCDVHAAIIVFSPAGKLHCFGEPNTDQILNSYINGTIEFDVSNSTGNSSTYKEYNKQYEEALKVLEMEKQKLADVENLTKIWNMGNWWNESIDEMNSDQLEEFMESISELKRKLLERADEHTNTMMFSML*