AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1386740

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1386740 MtrunA17_Chr2g0290341 82.136 515 29 3 24 505 22 506 0.0 855
Msa1386740 MtrunA17_Chr2g0290321 72.575 536 111 4 3 505 19 551 0.0 777
Msa1386740 MtrunA17_Chr7g0239471 71.230 504 112 5 2 505 17 487 0.0 669
Msa1386740 MtrunA17_Chr3g0120841 46.909 550 233 9 3 502 21 561 2.54e-150 441
Msa1386740 MtrunA17_Chr3g0120231 78.947 209 43 1 297 505 2 209 1.23e-111 329
Msa1386740 MtrunA17_Chr3g0120201 47.348 264 129 5 242 502 6 262 4.10e-73 232
Msa1386740 MtrunA17_Chr3g0120221 57.927 164 65 2 3 164 16 177 2.17e-61 199
Msa1386740 MtrunA17_Chr7g0238851 75.949 79 17 1 4 82 5 81 2.80e-29 110
Msa1386740 MtrunA17_Chr3g0120191 56.180 89 38 1 94 182 11 98 2.65e-28 108
Msa1386740 MtrunA17_Chr3g0120181 75.862 58 14 0 25 82 2 59 4.87e-28 106
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1386740 AT4G16420.2 54.545 506 185 8 3 504 18 482 0.0 536
Msa1386740 AT4G16420.1 54.545 506 189 8 3 504 18 486 0.0 534
Msa1386740 AT4G16420.3 54.813 509 182 9 3 504 18 485 0.0 532
Msa1386740 AT3G07740.1 42.351 536 264 10 3 503 20 545 5.38e-134 399
Msa1386740 AT3G07740.3 43.045 532 257 11 8 503 3 524 9.73e-133 395
Msa1386740 AT3G07740.4 41.805 543 264 11 3 503 20 552 3.54e-131 392
Msa1386740 AT3G07740.2 42.650 483 232 10 56 503 2 474 1.28e-117 355

Find 107 sgRNAs with CRISPR-Local

Find 197 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCTTCATTTCTTGAGGAATT+TGG 0.149009 tig0020858:-109037 None:intergenic
AGACAGAAGAATGCTTTATT+TGG 0.172431 tig0020858:+107289 Msa1386740:CDS
TTTGGAGATAATTCAGAATT+TGG 0.173645 tig0020858:+107307 Msa1386740:CDS
TCTCCAAAGGACGGAGATTT+TGG 0.187602 tig0020858:+109004 Msa1386740:CDS
AAATGTATGGCCTGGAAAAT+TGG 0.221477 tig0020858:+108400 Msa1386740:CDS
TCTTCATCTGCACTCCAGTC+TGG 0.242682 tig0020858:-108221 None:intergenic
CTTGAAGTAGCTGGCCTTGC+AGG 0.265434 tig0020858:-109824 None:intergenic
TTATCAAATGTTCAAGAATA+TGG 0.293541 tig0020858:+110101 Msa1386740:CDS
TTCGGAACCCCGTGATTGTT+CGG 0.302629 tig0020858:-109767 None:intergenic
TCAGCATCATCGTCATATTC+AGG 0.306131 tig0020858:-109112 None:intergenic
AAGGAGCAGGTGAAGCAAAA+TGG 0.330889 tig0020858:+107772 Msa1386740:CDS
CCTCCAAAATCTCCGTCCTT+TGG 0.332116 tig0020858:-109007 None:intergenic
GCATGAACACGTCATATTTC+CGG 0.336745 tig0020858:-109441 None:intergenic
ACAAAAGCAATCATGATTAC+AGG 0.344529 tig0020858:+107919 Msa1386740:CDS
TTATACGTATCTATAATAAA+AGG 0.350745 tig0020858:+109203 Msa1386740:CDS
CTCTCCGGTCTTCCCTCTTC+CGG 0.360280 tig0020858:+108497 Msa1386740:CDS
AGTAGCTGGCCTTGCAGGTC+TGG 0.368209 tig0020858:-109819 None:intergenic
TCGGAACCCCGTGATTGTTC+GGG 0.378631 tig0020858:-109766 None:intergenic
TGCAGCTGGTGCTAACAAAA+AGG 0.378782 tig0020858:+108876 Msa1386740:CDS
CTTCATCTGCACTCCAGTCT+GGG 0.385677 tig0020858:-108220 None:intergenic
TCAACACATAAGTCAAAATC+AGG 0.387672 tig0020858:-107864 None:intergenic
AGCTGAAGCAGATATATATC+TGG 0.388984 tig0020858:+109679 Msa1386740:CDS
GGTGTTAAATCCTTCTCAAA+TGG 0.404781 tig0020858:-109400 None:intergenic
GAATTTGGTGCTGCTGCTCC+AGG 0.409359 tig0020858:+107322 Msa1386740:CDS
GGATTTAACACCCGAGGAAA+AGG 0.418496 tig0020858:+109411 Msa1386740:CDS
ATGCTCATTAAGAAGGGTAT+TGG 0.420108 tig0020858:+110153 Msa1386740:CDS
TCCAATAAAGGCCGGGGAAA+AGG 0.423031 tig0020858:+108901 Msa1386740:CDS
TGAGAAATTACTCACTGATA+TGG 0.424714 tig0020858:+109132 Msa1386740:CDS
AACGTTTGTGCTGCGAGTTA+AGG 0.442285 tig0020858:+109995 Msa1386740:CDS
TTCCTCTCATCTCCCCAGAC+TGG 0.451454 tig0020858:+108207 Msa1386740:CDS
GGTCTGGTACCAGCAGAGTC+TGG 0.454618 tig0020858:-109803 None:intergenic
CTGTCGGTACAAATGATTGC+TGG 0.454974 tig0020858:+110054 Msa1386740:CDS
GGGAATTGAAATGTATGGCC+TGG 0.457047 tig0020858:+108392 Msa1386740:intron
GAACTTCTTGCAACGGCGAA+AGG 0.459906 tig0020858:+108646 Msa1386740:CDS
GTTCATGCGCTTTCATACTA+AGG 0.465103 tig0020858:+109456 Msa1386740:CDS
AAGAAATGAAGGCCGTACTT+TGG 0.471852 tig0020858:+109048 Msa1386740:CDS
AGGAGCAGGTGAAGCAAAAT+GGG 0.476294 tig0020858:+107773 Msa1386740:CDS
ATGTGGTCCCGAACAATCAC+GGG 0.476754 tig0020858:+109759 Msa1386740:CDS
AATGTATGGCCTGGAAAATT+GGG 0.480655 tig0020858:+108401 Msa1386740:CDS
TGGATCCCATGAAAATTGAC+AGG 0.487796 tig0020858:+110121 Msa1386740:CDS
TACTGCAAGAAAGATATTAC+CGG 0.489315 tig0020858:+107813 Msa1386740:CDS
AAAGAAGTGCTCGCGGGGCA+AGG 0.490951 tig0020858:+109726 Msa1386740:CDS
AACTTCTTGCAACGGCGAAA+GGG 0.492732 tig0020858:+108647 Msa1386740:CDS
AAAGGCATCCAATAAAGGCC+GGG 0.494520 tig0020858:+108894 Msa1386740:CDS
CTTGAACATTTGATAAGCAT+CGG 0.496085 tig0020858:-110094 None:intergenic
AGGGTCGGATTCTGGGTCCT+CGG 0.499489 tig0020858:+108840 Msa1386740:intron
GTATGATATGCTCATTAAGA+AGG 0.500449 tig0020858:+110146 Msa1386740:CDS
AGGACATGTCTCATGCTGTT+GGG 0.502752 tig0020858:+108611 Msa1386740:intron
GAAGCCTATCATACCGGAAG+AGG 0.505742 tig0020858:-108510 None:intergenic
CAGTTCTGAGCAATTCATCA+TGG 0.506351 tig0020858:-109483 None:intergenic
CAGCAATCATTTGTACCGAC+AGG 0.513175 tig0020858:-110053 None:intergenic
TAGGACATGTCTCATGCTGT+TGG 0.513693 tig0020858:+108610 Msa1386740:intron
CCAAATTCCTCAAGAAATGA+AGG 0.515725 tig0020858:+109037 Msa1386740:CDS
TCTGGAGACATCAATGCATT+CGG 0.516271 tig0020858:-109785 None:intergenic
GTCTACACACATGCTGCAGC+TGG 0.516899 tig0020858:+108862 Msa1386740:CDS
AAAAGGCATCCAATAAAGGC+CGG 0.525388 tig0020858:+108893 Msa1386740:CDS
GGCACACGGCACACTTGATA+CGG 0.529984 tig0020858:-107842 None:intergenic
TGGTTATAACCCGAAAAGAC+AGG 0.531230 tig0020858:+109081 Msa1386740:CDS
CTCTTGCATCTTCAGATACA+CGG 0.533130 tig0020858:-110028 None:intergenic
TTCATCTGCACTCCAGTCTG+GGG 0.535702 tig0020858:-108219 None:intergenic
TTGACGGCACTTGAAGTAGC+TGG 0.536839 tig0020858:-109833 None:intergenic
TAAGAACAAAGAGGCATGCA+TGG 0.541880 tig0020858:+108449 Msa1386740:CDS
AGTACGGCCTTCATTTCTTG+AGG 0.551492 tig0020858:-109044 None:intergenic
CATACCGGAAGAGGGAAGAC+CGG 0.552809 tig0020858:-108501 None:intergenic
TGCATCTTCAGATACACGGT+TGG 0.556814 tig0020858:-110024 None:intergenic
TTGATGTCTCCAGACTCTGC+TGG 0.561285 tig0020858:+109794 Msa1386740:CDS
CACTGGCTTCAACCAAAGTA+CGG 0.564700 tig0020858:-109060 None:intergenic
CTTGCAACGGCGAAAGGGCA+AGG 0.565938 tig0020858:+108652 Msa1386740:CDS
AAGAAGTGCTCGCGGGGCAA+GGG 0.565994 tig0020858:+109727 Msa1386740:CDS
CCGCAGGAAGCAATTGCTGC+TGG 0.571283 tig0020858:+109644 Msa1386740:intron
CGTACTTTGGTTGAAGCCAG+TGG 0.574934 tig0020858:+109061 Msa1386740:CDS
TGTGAGATAGGTCAAGGAGC+AGG 0.575240 tig0020858:+107759 Msa1386740:intron
ATATGAAAATCCATTTGAGA+AGG 0.582614 tig0020858:+109390 Msa1386740:CDS
CATGTGGTCCCGAACAATCA+CGG 0.583150 tig0020858:+109758 Msa1386740:CDS
GTCATTGACAGATTCTCCAA+AGG 0.585654 tig0020858:+108991 Msa1386740:intron
CCGAGGAAAAGGCAATATGC+CGG 0.591718 tig0020858:+109422 Msa1386740:CDS
AAGGCATCCAATAAAGGCCG+GGG 0.595048 tig0020858:+108895 Msa1386740:CDS
TGAAGAAATGTTGATTCTAG+AGG 0.597822 tig0020858:+108238 Msa1386740:CDS
CAAAAGCAATCATGATTACA+GGG 0.598809 tig0020858:+107920 Msa1386740:CDS
TATGATATGCTCATTAAGAA+GGG 0.601699 tig0020858:+110147 Msa1386740:CDS
CAATCATGATTACAGGGTCA+TGG 0.603132 tig0020858:+107926 Msa1386740:CDS
AAGCTGAAAGAAGTGCTCGC+GGG 0.610780 tig0020858:+109720 Msa1386740:CDS
CAGAGAAGAACTTCTTGCAA+CGG 0.616082 tig0020858:+108639 Msa1386740:CDS
AAGCCTATCATACCGGAAGA+GGG 0.618355 tig0020858:-108509 None:intergenic
GCAGAAGTAGCTGAGCATGT+TGG 0.618695 tig0020858:+108423 Msa1386740:CDS
GGGCAAGGTGATGACAAGAA+AGG 0.622437 tig0020858:+108667 Msa1386740:CDS
CTCCAGTCTGGGGAGATGAG+AGG 0.625328 tig0020858:-108209 None:intergenic
AGCTGAAAGAAGTGCTCGCG+GGG 0.625716 tig0020858:+109721 Msa1386740:CDS
TGAGAAGGATTTAACACCCG+AGG 0.635433 tig0020858:+109405 Msa1386740:CDS
TTGACAGATTCTCCAAAGGA+CGG 0.643226 tig0020858:+108995 Msa1386740:intron
GAAGCTGAAAGAAGTGCTCG+CGG 0.649791 tig0020858:+109719 Msa1386740:CDS
TAAGTCAAAATCAGGGCACA+CGG 0.654683 tig0020858:-107856 None:intergenic
GAAGATGCAAGAGGACCTGT+CGG 0.659393 tig0020858:+110038 Msa1386740:CDS
CGTGTATCTGAAGATGCAAG+AGG 0.659698 tig0020858:+110029 Msa1386740:CDS
GGCAAGGGAAAGTACTCATG+TGG 0.660030 tig0020858:+109742 Msa1386740:CDS
TAATGACAATGACACTGAGG+AGG 0.665463 tig0020858:+109159 Msa1386740:CDS
ACACTTGATACGGATTACTC+CGG 0.666906 tig0020858:-107832 None:intergenic
CAACACATAAGTCAAAATCA+GGG 0.668458 tig0020858:-107863 None:intergenic
TATCTGCTTCAGCTGAAGTG+CGG 0.668725 tig0020858:-109670 None:intergenic
GGCTTGATGAAAGAGAGCGC+AGG 0.673278 tig0020858:+109338 Msa1386740:intron
AGATTAAGAGAGGTCTCAGG+GGG 0.692267 tig0020858:-110178 None:intergenic
TGCAGCATGTGTGTAGACCG+AGG 0.695707 tig0020858:-108857 None:intergenic
GGATCCCATGAAAATTGACA+GGG 0.695744 tig0020858:+110122 Msa1386740:CDS
CTGCTGGTACCAGACCTGCA+AGG 0.696399 tig0020858:+109810 Msa1386740:CDS
TGTTGGAACTAAGAACAAAG+AGG 0.697393 tig0020858:+108440 Msa1386740:CDS
TGTGGTCCCGAACAATCACG+GGG 0.753110 tig0020858:+109760 Msa1386740:CDS
GTTTAATGACAATGACACTG+AGG 0.755990 tig0020858:+109156 Msa1386740:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TCTATTGTTTATATATTTTA+GGG + tig0020858:108821-108840 Msa1386740:intron 10.0%
!! TATAGAATTAATAAGTACAA+TGG - tig0020858:107369-107388 None:intergenic 15.0%
!!! TTTTCTTTTCATTTTCAAAA+AGG + tig0020858:107659-107678 Msa1386740:intron 15.0%
!! AATAATTTATGCTTGTTTAT+CGG + tig0020858:108065-108084 Msa1386740:intron 15.0%
!!! TAGTAATACAATTTTCTTAA+CGG - tig0020858:108099-108118 None:intergenic 15.0%
!!! TTTAGATTAGTTTTTTAACA+CGG + tig0020858:108158-108177 Msa1386740:intron 15.0%
!!! CTCTATTGTTTATATATTTT+AGG + tig0020858:108820-108839 Msa1386740:intron 15.0%
!! TTATACGTATCTATAATAAA+AGG + tig0020858:109203-109222 Msa1386740:CDS 15.0%
!!! ATAGTTTTAAAATGAAAACA+TGG - tig0020858:109919-109938 None:intergenic 15.0%
!!! TGTACTTTTATTTACTGTAT+AGG + tig0020858:107260-107279 Msa1386740:intron 20.0%
!!! GTACTTTTATTTACTGTATA+GGG + tig0020858:107261-107280 Msa1386740:intron 20.0%
!! AACTATAGCTATAAGTATTA+TGG + tig0020858:107416-107435 Msa1386740:intron 20.0%
!!! TTTAGCTTAAGATATGATAT+AGG + tig0020858:107621-107640 Msa1386740:intron 20.0%
!! AATGAAAAGAAAAAAATCCA+TGG - tig0020858:107652-107671 None:intergenic 20.0%
!!! ATTTTCAAAAAGGAAACATT+AGG + tig0020858:107669-107688 Msa1386740:intron 20.0%
!!! TTTTCAAAAAGGAAACATTA+GGG + tig0020858:107670-107689 Msa1386740:intron 20.0%
!! ATTCATTCATTTATGATTCA+TGG + tig0020858:107709-107728 Msa1386740:intron 20.0%
!!! TGAAAATAATTTTGTGAGAT+AGG + tig0020858:107747-107766 Msa1386740:intron 20.0%
!!! AGCTATAATATTTTCGTTTT+AGG + tig0020858:108371-108390 Msa1386740:intron 20.0%
!!! GCTATAATATTTTCGTTTTA+GGG + tig0020858:108372-108391 Msa1386740:intron 20.0%
!! TATAATATTCGTTCATATCA+AGG + tig0020858:108558-108577 Msa1386740:intron 20.0%
!!! TTGTTTATATATTTTAGGGT+CGG + tig0020858:108825-108844 Msa1386740:intron 20.0%
!!! AACAACCATTTTAGTTTTAT+AGG + tig0020858:109228-109247 Msa1386740:intron 20.0%
!! AGAAACCTATAAAACTAAAA+TGG - tig0020858:109236-109255 None:intergenic 20.0%
!!! ACATTTTTCAATTTGAAGAT+AGG - tig0020858:109314-109333 None:intergenic 20.0%
!! TTATCAAATGTTCAAGAATA+TGG + tig0020858:110101-110120 Msa1386740:CDS 20.0%
! TTTGGAGATAATTCAGAATT+TGG + tig0020858:107307-107326 Msa1386740:CDS 25.0%
! AGGAAAACATTTGAAAGTAA+TGG - tig0020858:107959-107978 None:intergenic 25.0%
!! TAATACAATTTTCTTAACGG+TGG - tig0020858:108096-108115 None:intergenic 25.0%
!! ATATGACAAAGCACTATATT+TGG + tig0020858:108309-108328 Msa1386740:intron 25.0%
!!! TTTTAGGGAATTGAAATGTA+TGG + tig0020858:108387-108406 Msa1386740:intron 25.0%
!!! GCTGATTTTTCTTATTTACT+AGG + tig0020858:108591-108610 Msa1386740:intron 25.0%
! AATGTCAATTGTGATTTATG+AGG - tig0020858:108943-108962 None:intergenic 25.0%
!!! AACAAATATGATTTTGAGTG+TGG - tig0020858:109272-109291 None:intergenic 25.0%
! TTCAAATTGAAAAATGTTGC+AGG + tig0020858:109317-109336 Msa1386740:intron 25.0%
! ATATGAAAATCCATTTGAGA+AGG + tig0020858:109390-109409 Msa1386740:CDS 25.0%
! GAATGTCCATAACAATTTAA+TGG - tig0020858:109569-109588 None:intergenic 25.0%
!!! ATTCACTTTTTTTCTCTACT+TGG + tig0020858:109585-109604 Msa1386740:intron 25.0%
!!! TTTTTTCTCTACTTGGAAAA+TGG + tig0020858:109592-109611 Msa1386740:intron 25.0%
!!! TTTTAAAATGAAAACATGGC+AGG - tig0020858:109915-109934 None:intergenic 25.0%
! TATGATATGCTCATTAAGAA+GGG + tig0020858:110147-110166 Msa1386740:CDS 25.0%
TTTATTTACTGTATAGGGCA+AGG + tig0020858:107266-107285 Msa1386740:intron 30.0%
! AGACAGAAGAATGCTTTATT+TGG + tig0020858:107289-107308 Msa1386740:CDS 30.0%
! TAATTTTGTGAGATAGGTCA+AGG + tig0020858:107753-107772 Msa1386740:intron 30.0%
TACTGCAAGAAAGATATTAC+CGG + tig0020858:107813-107832 Msa1386740:CDS 30.0%
CAACACATAAGTCAAAATCA+GGG - tig0020858:107866-107885 None:intergenic 30.0%
TCAACACATAAGTCAAAATC+AGG - tig0020858:107867-107886 None:intergenic 30.0%
!! TAATCATGATTGCTTTTGTG+GGG - tig0020858:107918-107937 None:intergenic 30.0%
!! GTAATCATGATTGCTTTTGT+GGG - tig0020858:107919-107938 None:intergenic 30.0%
!! TGTAATCATGATTGCTTTTG+TGG - tig0020858:107920-107939 None:intergenic 30.0%
ACAAAAGCAATCATGATTAC+AGG + tig0020858:107919-107938 Msa1386740:CDS 30.0%
CAAAAGCAATCATGATTACA+GGG + tig0020858:107920-107939 Msa1386740:CDS 30.0%
AGACAATTTATTCTGCAACA+TGG - tig0020858:108186-108205 None:intergenic 30.0%
!! TGAAGAAATGTTGATTCTAG+AGG + tig0020858:108238-108257 Msa1386740:CDS 30.0%
TTGGTTTAAACTAATTGCGT+AGG + tig0020858:108264-108283 Msa1386740:intron 30.0%
!! AACAAAAAGGCATCCAATAA+AGG + tig0020858:108889-108908 Msa1386740:CDS 30.0%
TGAGAAATTACTCACTGATA+TGG + tig0020858:109132-109151 Msa1386740:CDS 30.0%
! GAAAAAGATTCTAGAGACTA+AGG + tig0020858:109528-109547 Msa1386740:CDS 30.0%
TGGATGCCATTAAATTGTTA+TGG + tig0020858:109560-109579 Msa1386740:intron 30.0%
!!! ATTTTGTTTTCTGAAACCGT+CGG + tig0020858:109951-109970 Msa1386740:intron 30.0%
CTTGAACATTTGATAAGCAT+CGG - tig0020858:110097-110116 None:intergenic 30.0%
GTATGATATGCTCATTAAGA+AGG + tig0020858:110146-110165 Msa1386740:CDS 30.0%
!! ATGATAGTTGAAAAAGCACC+TGG - tig0020858:107343-107362 None:intergenic 35.0%
GAAATGGAAGAATGCAAACA+TGG - tig0020858:107464-107483 None:intergenic 35.0%
AAACAACAAGCAACGTGAAA+TGG - tig0020858:107480-107499 None:intergenic 35.0%
! ATATGATATAGGTGTTGCCA+TGG + tig0020858:107632-107651 Msa1386740:intron 35.0%
! CAATTTTCTTAACGGTGGTT+GGG - tig0020858:108091-108110 None:intergenic 35.0%
! ACAATTTTCTTAACGGTGGT+TGG - tig0020858:108092-108111 None:intergenic 35.0%
!! ATGTTGATTCTAGAGGTACT+TGG + tig0020858:108245-108264 Msa1386740:intron 35.0%
AACTAATTGCGTAGGAGTAA+CGG + tig0020858:108272-108291 Msa1386740:intron 35.0%
AAATGTATGGCCTGGAAAAT+TGG + tig0020858:108400-108419 Msa1386740:CDS 35.0%
AATGTATGGCCTGGAAAATT+GGG + tig0020858:108401-108420 Msa1386740:CDS 35.0%
TGTTGGAACTAAGAACAAAG+AGG + tig0020858:108440-108459 Msa1386740:CDS 35.0%
! GAAGTTTTACTTGAACTCTC+CGG + tig0020858:108482-108501 Msa1386740:CDS 35.0%
TTATTCGAAGCCTATCATAC+CGG - tig0020858:108519-108538 None:intergenic 35.0%
!!! TATATTTTAGGGTCGGATTC+TGG + tig0020858:108832-108851 Msa1386740:intron 35.0%
!!! ATATTTTAGGGTCGGATTCT+GGG + tig0020858:108833-108852 Msa1386740:intron 35.0%
TGTCAATGACAAAAGGACTA+GGG - tig0020858:108981-109000 None:intergenic 35.0%
GAGAATCTGTCAATGACAAA+AGG - tig0020858:108988-109007 None:intergenic 35.0%
CCTTCATTTCTTGAGGAATT+TGG - tig0020858:109040-109059 None:intergenic 35.0%
CCAAATTCCTCAAGAAATGA+AGG + tig0020858:109037-109056 Msa1386740:CDS 35.0%
GTTTAATGACAATGACACTG+AGG + tig0020858:109156-109175 Msa1386740:CDS 35.0%
GGTGTTAAATCCTTCTCAAA+TGG - tig0020858:109403-109422 None:intergenic 35.0%
AGCTGAAGCAGATATATATC+TGG + tig0020858:109679-109698 Msa1386740:CDS 35.0%
!! AGAGTGTTTTGTTGCATTGA+CGG - tig0020858:109852-109871 None:intergenic 35.0%
TGATAGAAAATTACAGCCGA+CGG - tig0020858:109970-109989 None:intergenic 35.0%
! TACACCCTGTCAATTTTCAT+GGG - tig0020858:110129-110148 None:intergenic 35.0%
! ATACACCCTGTCAATTTTCA+TGG - tig0020858:110130-110149 None:intergenic 35.0%
! ATGCTCATTAAGAAGGGTAT+TGG + tig0020858:110153-110172 Msa1386740:CDS 35.0%
! ACACTTGATACGGATTACTC+CGG - tig0020858:107835-107854 None:intergenic 40.0%
TAAGTCAAAATCAGGGCACA+CGG - tig0020858:107859-107878 None:intergenic 40.0%
! TGTGTTGAGTGCTTTTCTGT+TGG + tig0020858:107879-107898 Msa1386740:CDS 40.0%
CAATCATGATTACAGGGTCA+TGG + tig0020858:107926-107945 Msa1386740:CDS 40.0%
TCTCTCGATCAAGACATCAA+AGG - tig0020858:107979-107998 None:intergenic 40.0%
TGATGTCTTGATCGAGAGAA+AGG + tig0020858:107980-107999 Msa1386740:intron 40.0%
! TAAGAACAAAGAGGCATGCA+TGG + tig0020858:108449-108468 Msa1386740:CDS 40.0%
CAGAGAAGAACTTCTTGCAA+CGG + tig0020858:108639-108658 Msa1386740:CDS 40.0%
!! AAAAGGCATCCAATAAAGGC+CGG + tig0020858:108893-108912 Msa1386740:CDS 40.0%
! TAAAATGCACACCTTTTCCC+CGG - tig0020858:108915-108934 None:intergenic 40.0%
CTGTCAATGACAAAAGGACT+AGG - tig0020858:108982-109001 None:intergenic 40.0%
GTCATTGACAGATTCTCCAA+AGG + tig0020858:108991-109010 Msa1386740:intron 40.0%
TTGACAGATTCTCCAAAGGA+CGG + tig0020858:108995-109014 Msa1386740:intron 40.0%
AAGAAATGAAGGCCGTACTT+TGG + tig0020858:109048-109067 Msa1386740:CDS 40.0%
!!! TCTTTTCGGGTTATAACCAC+TGG - tig0020858:109080-109099 None:intergenic 40.0%
TGGTTATAACCCGAAAAGAC+AGG + tig0020858:109081-109100 Msa1386740:CDS 40.0%
! GATCGAATTCCTGTCTTTTC+GGG - tig0020858:109093-109112 None:intergenic 40.0%
! GGATCGAATTCCTGTCTTTT+CGG - tig0020858:109094-109113 None:intergenic 40.0%
TCAGCATCATCGTCATATTC+AGG - tig0020858:109115-109134 None:intergenic 40.0%
TAATGACAATGACACTGAGG+AGG + tig0020858:109159-109178 Msa1386740:CDS 40.0%
!! ATTTTGAGTGTGGATGACTC+AGG - tig0020858:109262-109281 None:intergenic 40.0%
GCATGAACACGTCATATTTC+CGG - tig0020858:109444-109463 None:intergenic 40.0%
GTTCATGCGCTTTCATACTA+AGG + tig0020858:109456-109475 Msa1386740:CDS 40.0%
!! CAGTTCTGAGCAATTCATCA+TGG - tig0020858:109486-109505 None:intergenic 40.0%
! AGAGACTAAGGTTTGAGACA+TGG + tig0020858:109540-109559 Msa1386740:intron 40.0%
TCTGGAGACATCAATGCATT+CGG - tig0020858:109788-109807 None:intergenic 40.0%
! GTCGGCTGTAATTTTCTATC+AGG + tig0020858:109969-109988 Msa1386740:intron 40.0%
CTCTTGCATCTTCAGATACA+CGG - tig0020858:110031-110050 None:intergenic 40.0%
TGGATCCCATGAAAATTGAC+AGG + tig0020858:110121-110140 Msa1386740:CDS 40.0%
GGATCCCATGAAAATTGACA+GGG + tig0020858:110122-110141 Msa1386740:CDS 40.0%
CAACGCGGAAAGTTACACAT+AGG - tig0020858:107526-107545 None:intergenic 45.0%
CTATGTGTAACTTTCCGCGT+TGG + tig0020858:107524-107543 Msa1386740:intron 45.0%
GCGCGTTTCTTCACTTTGTT+TGG + tig0020858:107563-107582 Msa1386740:intron 45.0%
AAGGAGCAGGTGAAGCAAAA+TGG + tig0020858:107772-107791 Msa1386740:CDS 45.0%
AGGAGCAGGTGAAGCAAAAT+GGG + tig0020858:107773-107792 Msa1386740:CDS 45.0%
GGGAATTGAAATGTATGGCC+TGG + tig0020858:108392-108411 Msa1386740:intron 45.0%
! CTACTTCTGCCCAATTTTCC+AGG - tig0020858:108413-108432 None:intergenic 45.0%
AAGCCTATCATACCGGAAGA+GGG - tig0020858:108512-108531 None:intergenic 45.0%
TAGGACATGTCTCATGCTGT+TGG + tig0020858:108610-108629 Msa1386740:intron 45.0%
AGGACATGTCTCATGCTGTT+GGG + tig0020858:108611-108630 Msa1386740:intron 45.0%
AACTTCTTGCAACGGCGAAA+GGG + tig0020858:108647-108666 Msa1386740:CDS 45.0%
! TGCAGCTGGTGCTAACAAAA+AGG + tig0020858:108876-108895 Msa1386740:CDS 45.0%
!! AAAGGCATCCAATAAAGGCC+GGG + tig0020858:108894-108913 Msa1386740:CDS 45.0%
! TCTCCAAAGGACGGAGATTT+TGG + tig0020858:109004-109023 Msa1386740:CDS 45.0%
AGTACGGCCTTCATTTCTTG+AGG - tig0020858:109047-109066 None:intergenic 45.0%
!! CACTGGCTTCAACCAAAGTA+CGG - tig0020858:109063-109082 None:intergenic 45.0%
TGAGAAGGATTTAACACCCG+AGG + tig0020858:109405-109424 Msa1386740:CDS 45.0%
GGATTTAACACCCGAGGAAA+AGG + tig0020858:109411-109430 Msa1386740:CDS 45.0%
! CATTTGTTGAACATGTCCGC+AGG + tig0020858:109628-109647 Msa1386740:intron 45.0%
TATCTGCTTCAGCTGAAGTG+CGG - tig0020858:109673-109692 None:intergenic 45.0%
AACGTTTGTGCTGCGAGTTA+AGG + tig0020858:109995-110014 Msa1386740:CDS 45.0%
TGCATCTTCAGATACACGGT+TGG - tig0020858:110027-110046 None:intergenic 45.0%
CGTGTATCTGAAGATGCAAG+AGG + tig0020858:110029-110048 Msa1386740:CDS 45.0%
! CAGCAATCATTTGTACCGAC+AGG - tig0020858:110056-110075 None:intergenic 45.0%
CTGTCGGTACAAATGATTGC+TGG + tig0020858:110054-110073 Msa1386740:CDS 45.0%
TGTGAGATAGGTCAAGGAGC+AGG + tig0020858:107759-107778 Msa1386740:intron 50.0%
TTCATCTGCACTCCAGTCTG+GGG - tig0020858:108222-108241 None:intergenic 50.0%
CTTCATCTGCACTCCAGTCT+GGG - tig0020858:108223-108242 None:intergenic 50.0%
TCTTCATCTGCACTCCAGTC+TGG - tig0020858:108224-108243 None:intergenic 50.0%
GTAGGAGTAACGGTGACTAG+TGG + tig0020858:108282-108301 Msa1386740:intron 50.0%
GCAGAAGTAGCTGAGCATGT+TGG + tig0020858:108423-108442 Msa1386740:CDS 50.0%
CTTCCCTCTTCCGGTATGAT+AGG + tig0020858:108506-108525 Msa1386740:intron 50.0%
GAAGCCTATCATACCGGAAG+AGG - tig0020858:108513-108532 None:intergenic 50.0%
GAACTTCTTGCAACGGCGAA+AGG + tig0020858:108646-108665 Msa1386740:CDS 50.0%
GGGCAAGGTGATGACAAGAA+AGG + tig0020858:108667-108686 Msa1386740:CDS 50.0%
! AAGGCATCCAATAAAGGCCG+GGG + tig0020858:108895-108914 Msa1386740:CDS 50.0%
TCCAATAAAGGCCGGGGAAA+AGG + tig0020858:108901-108920 Msa1386740:CDS 50.0%
! ACCTTTTCCCCGGCCTTTAT+TGG - tig0020858:108905-108924 None:intergenic 50.0%
CCTCCAAAATCTCCGTCCTT+TGG - tig0020858:109010-109029 None:intergenic 50.0%
! CCAAAGGACGGAGATTTTGG+AGG + tig0020858:109007-109026 Msa1386740:CDS 50.0%
CGTACTTTGGTTGAAGCCAG+TGG + tig0020858:109061-109080 Msa1386740:CDS 50.0%
! CGGCATATTGCCTTTTCCTC+GGG - tig0020858:109424-109443 None:intergenic 50.0%
! CCGGCATATTGCCTTTTCCT+CGG - tig0020858:109425-109444 None:intergenic 50.0%
CCGAGGAAAAGGCAATATGC+CGG + tig0020858:109422-109441 Msa1386740:CDS 50.0%
!! GAAGCTGAAAGAAGTGCTCG+CGG + tig0020858:109719-109738 Msa1386740:CDS 50.0%
!! AAGCTGAAAGAAGTGCTCGC+GGG + tig0020858:109720-109739 Msa1386740:CDS 50.0%
!! GGCAAGGGAAAGTACTCATG+TGG + tig0020858:109742-109761 Msa1386740:CDS 50.0%
CATGTGGTCCCGAACAATCA+CGG + tig0020858:109758-109777 Msa1386740:CDS 50.0%
ATGTGGTCCCGAACAATCAC+GGG + tig0020858:109759-109778 Msa1386740:CDS 50.0%
TTCGGAACCCCGTGATTGTT+CGG - tig0020858:109770-109789 None:intergenic 50.0%
TTGATGTCTCCAGACTCTGC+TGG + tig0020858:109794-109813 Msa1386740:CDS 50.0%
TTGACGGCACTTGAAGTAGC+TGG - tig0020858:109836-109855 None:intergenic 50.0%
GAAGATGCAAGAGGACCTGT+CGG + tig0020858:110038-110057 Msa1386740:CDS 50.0%
!! GAATTTGGTGCTGCTGCTCC+AGG + tig0020858:107322-107341 Msa1386740:CDS 55.0%
!! GATGGTGGACAGTTCCAACG+CGG - tig0020858:107541-107560 None:intergenic 55.0%
AAGTGAAGAAACGCGCGCGA+TGG - tig0020858:107559-107578 None:intergenic 55.0%
GGCACACGGCACACTTGATA+CGG - tig0020858:107845-107864 None:intergenic 55.0%
TTCCTCTCATCTCCCCAGAC+TGG + tig0020858:108207-108226 Msa1386740:CDS 55.0%
CATACCGGAAGAGGGAAGAC+CGG - tig0020858:108504-108523 None:intergenic 55.0%
TGCAGCATGTGTGTAGACCG+AGG - tig0020858:108860-108879 None:intergenic 55.0%
GTCTACACACATGCTGCAGC+TGG + tig0020858:108862-108881 Msa1386740:CDS 55.0%
! GGCTTGATGAAAGAGAGCGC+AGG + tig0020858:109338-109357 Msa1386740:intron 55.0%
CCAGCAGCAATTGCTTCCTG+CGG - tig0020858:109647-109666 None:intergenic 55.0%
!! AGCTGAAAGAAGTGCTCGCG+GGG + tig0020858:109721-109740 Msa1386740:CDS 55.0%
TGTGGTCCCGAACAATCACG+GGG + tig0020858:109760-109779 Msa1386740:CDS 55.0%
TCGGAACCCCGTGATTGTTC+GGG - tig0020858:109769-109788 None:intergenic 55.0%
!! CTTGAAGTAGCTGGCCTTGC+AGG - tig0020858:109827-109846 None:intergenic 55.0%
TGAAGAAACGCGCGCGATGG+TGG - tig0020858:107556-107575 None:intergenic 60.0%
CTCCAGTCTGGGGAGATGAG+AGG - tig0020858:108212-108231 None:intergenic 60.0%
CTCTCCGGTCTTCCCTCTTC+CGG + tig0020858:108497-108516 Msa1386740:CDS 60.0%
CTTGCAACGGCGAAAGGGCA+AGG + tig0020858:108652-108671 Msa1386740:CDS 60.0%
AGGGTCGGATTCTGGGTCCT+CGG + tig0020858:108840-108859 Msa1386740:intron 60.0%
CCGCAGGAAGCAATTGCTGC+TGG + tig0020858:109644-109663 Msa1386740:intron 60.0%
!! AAAGAAGTGCTCGCGGGGCA+AGG + tig0020858:109726-109745 Msa1386740:CDS 60.0%
!! AAGAAGTGCTCGCGGGGCAA+GGG + tig0020858:109727-109746 Msa1386740:CDS 60.0%
GGTCTGGTACCAGCAGAGTC+TGG - tig0020858:109806-109825 None:intergenic 60.0%
CTGCTGGTACCAGACCTGCA+AGG + tig0020858:109810-109829 Msa1386740:CDS 60.0%
!! AGTAGCTGGCCTTGCAGGTC+TGG - tig0020858:109822-109841 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
tig0020858 gene 107254 110185 107254 ID=Msa1386740;Name=Msa1386740
tig0020858 mRNA 107254 110185 107254 ID=Msa1386740-mRNA-1;Parent=Msa1386740;Name=Msa1386740-mRNA-1;_AED=0.13;_eAED=0.13;_QI=0|0|0|0.81|0.9|0.90|11|0|505
tig0020858 exon 107254 107258 107254 ID=Msa1386740-mRNA-1:exon:10321;Parent=Msa1386740-mRNA-1
tig0020858 exon 107282 107343 107282 ID=Msa1386740-mRNA-1:exon:10322;Parent=Msa1386740-mRNA-1
tig0020858 exon 107769 107947 107769 ID=Msa1386740-mRNA-1:exon:10323;Parent=Msa1386740-mRNA-1
tig0020858 exon 108194 108259 108194 ID=Msa1386740-mRNA-1:exon:10324;Parent=Msa1386740-mRNA-1
tig0020858 exon 108393 108518 108393 ID=Msa1386740-mRNA-1:exon:10325;Parent=Msa1386740-mRNA-1
tig0020858 exon 108613 108736 108613 ID=Msa1386740-mRNA-1:exon:10326;Parent=Msa1386740-mRNA-1
tig0020858 exon 108842 108922 108842 ID=Msa1386740-mRNA-1:exon:10327;Parent=Msa1386740-mRNA-1
tig0020858 exon 109002 109224 109002 ID=Msa1386740-mRNA-1:exon:10328;Parent=Msa1386740-mRNA-1
tig0020858 exon 109339 109549 109339 ID=Msa1386740-mRNA-1:exon:10329;Parent=Msa1386740-mRNA-1
tig0020858 exon 109650 109895 109650 ID=Msa1386740-mRNA-1:exon:10330;Parent=Msa1386740-mRNA-1
tig0020858 exon 109991 110185 109991 ID=Msa1386740-mRNA-1:exon:10331;Parent=Msa1386740-mRNA-1
tig0020858 CDS 107254 107258 107254 ID=Msa1386740-mRNA-1:cds;Parent=Msa1386740-mRNA-1
tig0020858 CDS 107282 107343 107282 ID=Msa1386740-mRNA-1:cds;Parent=Msa1386740-mRNA-1
tig0020858 CDS 107769 107947 107769 ID=Msa1386740-mRNA-1:cds;Parent=Msa1386740-mRNA-1
tig0020858 CDS 108194 108259 108194 ID=Msa1386740-mRNA-1:cds;Parent=Msa1386740-mRNA-1
tig0020858 CDS 108393 108518 108393 ID=Msa1386740-mRNA-1:cds;Parent=Msa1386740-mRNA-1
tig0020858 CDS 108613 108736 108613 ID=Msa1386740-mRNA-1:cds;Parent=Msa1386740-mRNA-1
tig0020858 CDS 108842 108922 108842 ID=Msa1386740-mRNA-1:cds;Parent=Msa1386740-mRNA-1
tig0020858 CDS 109002 109224 109002 ID=Msa1386740-mRNA-1:cds;Parent=Msa1386740-mRNA-1
tig0020858 CDS 109339 109549 109339 ID=Msa1386740-mRNA-1:cds;Parent=Msa1386740-mRNA-1
tig0020858 CDS 109650 109895 109650 ID=Msa1386740-mRNA-1:cds;Parent=Msa1386740-mRNA-1
tig0020858 CDS 109991 110185 109991 ID=Msa1386740-mRNA-1:cds;Parent=Msa1386740-mRNA-1
Gene Sequence

>Msa1386740

ATGATGGCAAGGAGACAGAAGAATGCTTTATTTGGAGATAATTCAGAATTTGGTGCTGCTGCTCCAGGTCAAGGAGCAGGTGAAGCAAAATGGGCTCTGTATCATTGCAATTACTGCAAGAAAGATATTACCGGAGTAATCCGTATCAAGTGTGCCGTGTGCCCTGATTTTGACTTATGTGTTGAGTGCTTTTCTGTTGGAGCTGAGCTCACACCCCACAAAAGCAATCATGATTACAGGGTCATGAATAAATTGTCTTTTCCTCTCATCTCCCCAGACTGGAGTGCAGATGAAGAAATGTTGATTCTAGAGGGAATTGAAATGTATGGCCTGGAAAATTGGGCAGAAGTAGCTGAGCATGTTGGAACTAAGAACAAAGAGGCATGCATGGAACACTATAAGAAGTTTTACTTGAACTCTCCGGTCTTCCCTCTTCCGGACATGTCTCATGCTGTTGGGAGAAGCAGAGAAGAACTTCTTGCAACGGCGAAAGGGCAAGGTGATGACAAGAAAGGTTTATCACAATACACGACATCACATTCGTTAGTTTATGTAGTTTTTAGGTCGGATTCTGGGTCCTCGGTCTACACACATGCTGCAGCTGGTGCTAACAAAAAGGCATCCAATAAAGGCCGGGGAAAAGATTCTCCAAAGGACGGAGATTTTGGAGGAAATAAGCCAAATTCCTCAAGAAATGAAGGCCGTACTTTGGTTGAAGCCAGTGGTTATAACCCGAAAAGACAGGAATTCGATCCTGAATATGACGATGATGCTGAGAAATTACTCACTGATATGGAGTTTAATGACAATGACACTGAGGAGGAGATAGAGCTCAAACTTCATATTATACGTATCTATAATAAAAGGCTTGATGAAAGAGAGCGCAGGAAGAAATTCATACTTGAAAGAAATTTGCTATATGAAAATCCATTTGAGAAGGATTTAACACCCGAGGAAAAGGCAATATGCCGGAAATATGACGTGTTCATGCGCTTTCATACTAAGGAAAACCATGATGAATTGCTCAGAACTGTTATCTCTGAACATAGATATCTGAAAAAGATTCTAGAGACTAAGGAAGCAATTGCTGCTGGTTGCCGCACTTCAGCTGAAGCAGATATATATCTGGCAAATAAGAGAAGAAATGAAGCTGAAAGAAGTGCTCGCGGGGCAAGGGAAAGTACTCATGTGGTCCCGAACAATCACGGGGTTCCGAATGCATTGATGTCTCCAGACTCTGCTGGTACCAGACCTGCAAGGCCAGCTACTTCAAGTGCCGTCAATGCAACAAAACACTCTGCAGCAGATTTACTTTCTGAAGTTGAGAAACGTTTGTGCTGCGAGTTAAGGTTGTCTCCAACCGTGTATCTGAAGATGCAAGAGGACCTGTCGGTACAAATGATTGCTGGAAGCGTCTCTTCAAAGTCCGATGCTTATCAAATGTTCAAGAATATGGATCCCATGAAAATTGACAGGGTGTATGATATGCTCATTAAGAAGGGTATTGGTTCCCCCTGA
Protein sequence

>Msa1386740

MMARRQKNALFGDNSEFGAAAPGQGAGEAKWALYHCNYCKKDITGVIRIKCAVCPDFDLCVECFSVGAELTPHKSNHDYRVMNKLSFPLISPDWSADEEMLILEGIEMYGLENWAEVAEHVGTKNKEACMEHYKKFYLNSPVFPLPDMSHAVGRSREELLATAKGQGDDKKGLSQYTTSHSLVYVVFRSDSGSSVYTHAAAGANKKASNKGRGKDSPKDGDFGGNKPNSSRNEGRTLVEASGYNPKRQEFDPEYDDDAEKLLTDMEFNDNDTEEEIELKLHIIRIYNKRLDERERRKKFILERNLLYENPFEKDLTPEEKAICRKYDVFMRFHTKENHDELLRTVISEHRYLKKILETKEAIAAGCRTSAEADIYLANKRRNEAERSARGARESTHVVPNNHGVPNALMSPDSAGTRPARPATSSAVNATKHSAADLLSEVEKRLCCELRLSPTVYLKMQEDLSVQMIAGSVSSKSDAYQMFKNMDPMKIDRVYDMLIKKGIGSP*