AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1386860

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1386860 MtrunA17_Chr4g0060841 90.730 356 7 1 1 330 1 356 0.0 655
Msa1386860 MtrunA17_Chr3g0134451 35.211 213 106 8 1 191 1 203 2.56e-26 106
Msa1386860 MtrunA17_Chr5g0417661 70.149 67 20 0 1 67 56 122 2.80e-25 98.6
Msa1386860 MtrunA17_Chr5g0417671 81.132 53 10 0 59 111 26 78 4.06e-23 91.7
Msa1386860 MtrunA17_Chr5g0425191 62.295 61 23 0 1 61 1 61 2.33e-21 87.4
Msa1386860 MtrunA17_Chr4g0044071 63.934 61 22 0 1 61 1 61 1.23e-20 85.9
Msa1386860 MtrunA17_Chr5g0427401 39.456 147 79 4 1 143 1 141 2.68e-18 82.4
Msa1386860 MtrunA17_Chr1g0159661 39.423 104 63 0 1 104 1 104 4.57e-17 77.8
Msa1386860 MtrunA17_Chr3g0125241 62.264 53 20 0 1 53 26 78 4.32e-16 75.9
Msa1386860 MtrunA17_Chr1g0173161 58.491 53 22 0 1 53 1 53 7.86e-16 71.6
Msa1386860 MtrunA17_Chr5g0412461 35.811 148 75 5 1 138 1 138 3.32e-15 73.9
Msa1386860 MtrunA17_Chr5g0412271 38.028 142 80 5 1 138 1 138 3.34e-15 73.9
Msa1386860 MtrunA17_Chr7g0246431 31.176 170 85 5 1 154 1 154 4.13e-15 73.2
Msa1386860 MtrunA17_Chr4g0020221 46.479 71 36 1 1 71 1 69 5.92e-15 73.6
Msa1386860 MtrunA17_Chr1g0164791 32.099 162 93 5 1 147 1 160 6.59e-15 73.2
Msa1386860 MtrunA17_Chr4g0057151 34.783 161 89 6 1 153 1 153 6.98e-15 72.4
Msa1386860 MtrunA17_Chr3g0142191 56.604 53 23 0 1 53 1 53 7.21e-15 72.8
Msa1386860 MtrunA17_Chr2g0308251 54.717 53 24 0 1 53 1 53 7.31e-15 69.3
Msa1386860 MtrunA17_Chr8g0351041 30.719 153 101 2 1 150 1 151 7.41e-15 71.2
Msa1386860 MtrunA17_Chr2g0279821 46.479 71 36 1 1 71 1 69 7.85e-15 73.2
Msa1386860 MtrunA17_Chr2g0284911 45.070 71 37 1 1 71 16 84 7.95e-15 73.2
Msa1386860 MtrunA17_Chr5g0427921 50.943 53 26 0 1 53 1 53 8.22e-15 68.2
Msa1386860 MtrunA17_Chr5g0420081 45.570 79 42 1 1 79 1 78 8.39e-15 72.8
Msa1386860 MtrunA17_Chr8g0366231 43.038 79 44 1 1 79 1 78 1.28e-14 72.4
Msa1386860 MtrunA17_Chr3g0097101 54.717 53 24 0 1 53 16 68 1.82e-14 72.0
Msa1386860 MtrunA17_Chr4g0061691 43.038 79 44 1 1 79 1 78 2.41e-14 71.6
Msa1386860 MtrunA17_Chr3g0077501 54.717 53 24 0 1 53 1 53 2.42e-14 71.2
Msa1386860 MtrunA17_Chr4g0049901 48.571 70 34 1 1 70 27 94 3.00e-14 71.2
Msa1386860 MtrunA17_Chr8g0380021 33.562 146 89 5 1 142 17 158 3.55e-14 71.2
Msa1386860 MtrunA17_Chr7g0246381 54.717 53 24 0 1 53 1 53 5.35e-14 66.2
Msa1386860 MtrunA17_Chr4g0049541 45.946 74 38 1 1 74 1 72 6.60e-14 70.1
Msa1386860 MtrunA17_Chr5g0411691 43.662 71 38 1 1 71 1 69 7.82e-14 70.1
Msa1386860 MtrunA17_Chr1g0204111 46.575 73 37 1 1 73 17 87 1.01e-13 70.1
Msa1386860 MtrunA17_Chr8g0351031 30.128 156 104 2 1 153 1 154 1.64e-13 68.9
Msa1386860 MtrunA17_Chr8g0377561 50.943 53 26 0 1 53 1 53 1.68e-13 65.9
Msa1386860 MtrunA17_Chr1g0175611 38.961 77 45 1 1 77 1 75 2.91e-13 67.0
Msa1386860 MtrunA17_Chr7g0220121 30.612 147 91 4 1 142 1 141 3.31e-13 68.6
Msa1386860 MtrunA17_Chr4g0061671 50.943 53 26 0 1 53 1 53 3.69e-13 67.0
Msa1386860 MtrunA17_Chr8g0351051 52.830 53 25 0 1 53 1 53 4.19e-13 68.2
Msa1386860 MtrunA17_Chr7g0220111 50.943 53 26 0 1 53 1 53 5.66e-13 67.8
Msa1386860 MtrunA17_Chr5g0420091 52.830 53 25 0 1 53 1 53 7.18e-13 67.0
Msa1386860 MtrunA17_Chr1g0208561 42.667 75 41 1 2 76 9 81 7.76e-13 67.0
Msa1386860 MtrunA17_Chr5g0404951 33.544 158 72 6 1 154 1 129 8.33e-13 67.0
Msa1386860 MtrunA17_Chr8g0379021 51.923 52 25 0 2 53 12 63 8.55e-13 65.9
Msa1386860 MtrunA17_Chr3g0089141 42.667 75 41 1 2 76 9 81 8.71e-13 67.0
Msa1386860 MtrunA17_Chr1g0166611 37.363 91 51 2 16 104 20 106 9.90e-13 64.7
Msa1386860 MtrunA17_Chr6g0455511 50.943 53 26 0 1 53 1 53 1.80e-12 66.2
Msa1386860 MtrunA17_Chr3g0122251 50.943 53 26 0 1 53 1 53 2.20e-12 66.2
Msa1386860 MtrunA17_Chr4g0052081 53.704 54 25 0 1 54 1 54 2.63e-12 64.7
Msa1386860 MtrunA17_Chr6g0474371 43.243 74 39 2 1 73 1 72 2.77e-12 64.7
Msa1386860 MtrunA17_Chr1g0208581 30.625 160 87 5 2 141 9 164 3.22e-12 65.5
Msa1386860 MtrunA17_Chr2g0333981 50.943 53 26 0 1 53 11 63 3.32e-12 64.3
Msa1386860 MtrunA17_Chr4g0018421 41.333 75 43 1 1 75 1 74 3.71e-12 64.7
Msa1386860 MtrunA17_Chr4g0074101 40.260 77 44 1 1 77 1 75 8.60e-12 63.5
Msa1386860 MtrunA17_Chr1g0175601 36.709 79 48 1 1 79 1 77 1.13e-11 62.8
Msa1386860 MtrunA17_Chr8g0357771 28.485 165 83 4 1 161 1 134 1.31e-11 62.8
Msa1386860 MtrunA17_Chr3g0099421 39.326 89 49 3 2 89 34 118 1.58e-11 63.9
Msa1386860 MtrunA17_Chr4g0018951 31.538 130 74 3 14 133 24 148 1.73e-11 62.0
Msa1386860 MtrunA17_Chr8g0355091 38.202 89 50 3 2 89 9 93 2.15e-11 63.2
Msa1386860 MtrunA17_Chr8g0376821 46.296 54 29 0 1 54 1 54 2.26e-11 62.0
Msa1386860 MtrunA17_Chr1g0180591 37.662 77 46 1 1 77 1 75 2.62e-11 61.6
Msa1386860 MtrunA17_Chr5g0419601 39.744 78 45 1 1 78 8 83 2.70e-11 62.8
Msa1386860 MtrunA17_Chr1g0206701 38.202 89 50 3 2 89 9 93 2.76e-11 62.8
Msa1386860 MtrunA17_Chr2g0317451 38.043 92 54 2 2 93 9 97 2.92e-11 62.8
Msa1386860 MtrunA17_Chr2g0317481 38.043 92 54 2 2 93 9 97 3.24e-11 62.4
Msa1386860 MtrunA17_Chr3g0099411 38.202 89 50 3 2 89 9 93 4.21e-11 62.0
Msa1386860 MtrunA17_Chr2g0292791 38.202 89 50 3 2 89 9 93 4.69e-11 62.0
Msa1386860 MtrunA17_Chr1g0169761 46.296 54 29 0 1 54 1 54 5.42e-11 60.8
Msa1386860 MtrunA17_Chr4g0052061 48.148 54 28 0 1 54 1 54 5.99e-11 60.1
Msa1386860 MtrunA17_Chr1g0206721 38.202 89 50 3 2 89 9 93 6.17e-11 61.6
Msa1386860 MtrunA17_Chr2g0325971 40.000 75 43 1 2 76 9 81 6.89e-11 61.6
Msa1386860 MtrunA17_Chr5g0422941 42.857 77 41 2 2 77 9 83 7.46e-11 61.6
Msa1386860 MtrunA17_Chr1g0188671 42.857 77 41 2 2 77 47 121 8.47e-11 62.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1386860 AT1G18750.4 48.173 301 111 7 1 270 1 287 1.17e-82 255
Msa1386860 AT1G18750.1 48.173 301 111 7 1 270 1 287 1.98e-82 255
Msa1386860 AT2G03060.3 45.614 285 105 6 1 250 1 270 4.25e-71 226
Msa1386860 AT2G03060.7 45.614 285 105 6 1 250 1 270 4.25e-71 226
Msa1386860 AT2G03060.1 45.614 285 105 6 1 250 1 270 4.25e-71 226
Msa1386860 AT2G03060.2 45.614 285 105 6 1 250 1 270 5.20e-71 226
Msa1386860 AT1G18750.3 42.216 334 115 11 1 270 1 320 6.12e-68 219
Msa1386860 AT1G69540.1 39.088 307 131 8 1 266 1 292 1.17e-57 190
Msa1386860 AT1G69540.2 35.526 380 173 13 1 329 1 359 1.21e-57 191
Msa1386860 AT1G18750.2 40.784 255 106 7 47 270 1 241 1.80e-51 174
Msa1386860 AT2G03060.6 34.804 204 83 6 82 250 24 212 3.45e-25 103
Msa1386860 AT2G03060.5 34.804 204 83 6 82 250 24 212 3.45e-25 103
Msa1386860 AT2G03060.4 34.804 204 83 6 82 250 24 212 3.45e-25 103
Msa1386860 AT1G77980.1 42.222 135 62 4 1 123 1 131 3.34e-24 101
Msa1386860 AT1G22130.1 55.696 79 34 1 1 79 1 78 3.05e-23 98.6
Msa1386860 AT1G77950.3 43.220 118 52 4 1 108 1 113 6.18e-22 93.6
Msa1386860 AT1G77950.2 43.220 118 52 4 1 108 1 113 6.18e-22 93.6
Msa1386860 AT1G77950.1 43.220 118 52 4 1 108 1 113 6.18e-22 93.6
Msa1386860 AT1G77950.4 43.220 118 52 4 1 108 1 113 6.18e-22 93.6
Msa1386860 AT1G18750.6 34.337 166 90 6 110 270 46 197 6.13e-20 88.6
Msa1386860 AT1G18750.5 34.337 166 90 6 110 270 34 185 6.74e-20 88.6
Msa1386860 AT2G26320.1 47.561 82 43 0 1 82 17 98 3.36e-19 82.0
Msa1386860 AT5G13790.1 38.384 99 57 2 1 97 1 97 6.22e-16 77.0
Msa1386860 AT2G03710.1 37.762 143 74 5 1 133 1 138 7.40e-16 76.6
Msa1386860 AT2G03710.2 37.762 143 74 5 1 133 1 138 8.12e-16 76.6
Msa1386860 AT4G24540.1 47.222 72 36 1 1 72 1 70 9.44e-16 75.5
Msa1386860 AT5G23260.3 47.887 71 37 0 1 71 1 71 1.59e-15 75.5
Msa1386860 AT5G23260.2 47.887 71 37 0 1 71 1 71 1.60e-15 75.5
Msa1386860 AT5G23260.4 47.887 71 37 0 1 71 17 87 2.20e-15 75.5
Msa1386860 AT5G23260.1 47.887 71 37 0 1 71 17 87 2.27e-15 75.5
Msa1386860 AT3G57390.3 42.353 85 47 1 1 85 1 83 2.81e-15 74.3
Msa1386860 AT2G03710.3 37.762 143 74 5 1 133 1 138 2.85e-15 73.6
Msa1386860 AT3G57390.1 42.353 85 47 1 1 85 1 83 3.65e-15 74.7
Msa1386860 AT4G09960.2 30.000 150 91 4 1 143 1 143 4.25e-15 73.6
Msa1386860 AT5G13790.2 43.056 72 39 1 1 72 1 70 4.81e-15 74.7
Msa1386860 AT3G61120.1 29.388 245 138 9 1 235 1 220 5.74e-15 73.9
Msa1386860 AT3G57230.2 44.444 72 38 1 1 72 1 70 6.71e-15 71.2
Msa1386860 AT4G09960.1 29.268 164 97 5 1 157 1 152 7.18e-15 73.2
Msa1386860 AT4G09960.5 29.268 164 97 5 1 157 11 162 7.67e-15 73.6
Msa1386860 AT4G09960.6 29.268 164 97 5 1 157 2 153 8.62e-15 73.2
Msa1386860 AT2G45660.2 31.056 161 97 5 1 154 1 154 1.41e-14 70.9
Msa1386860 AT1G26310.1 43.038 79 44 1 1 79 1 78 1.78e-14 72.8
Msa1386860 AT2G45660.1 31.250 160 96 5 1 153 1 153 1.81e-14 72.0
Msa1386860 AT3G57230.1 44.444 72 38 1 1 72 1 70 2.07e-14 72.4
Msa1386860 AT3G57230.4 44.444 72 38 1 1 72 1 70 2.07e-14 72.4
Msa1386860 AT2G14210.1 43.590 78 43 1 1 78 1 77 2.13e-14 72.0
Msa1386860 AT2G22540.3 45.714 70 36 1 1 70 1 68 2.19e-14 72.0
Msa1386860 AT2G22540.1 45.714 70 36 1 1 70 1 68 2.19e-14 72.0
Msa1386860 AT2G14210.2 43.590 78 43 1 1 78 1 77 2.31e-14 72.0
Msa1386860 AT4G09960.3 29.268 164 97 5 1 157 27 178 2.48e-14 72.4
Msa1386860 AT4G11880.2 29.897 194 110 9 1 187 1 175 2.53e-14 70.9
Msa1386860 AT4G11880.3 29.897 194 110 9 1 187 1 175 2.53e-14 70.9
Msa1386860 AT4G37940.3 45.070 71 37 1 1 71 1 69 2.61e-14 70.5
Msa1386860 AT5G51870.2 45.333 75 39 1 1 75 1 73 3.46e-14 70.1
Msa1386860 AT4G37940.4 45.070 71 37 1 1 71 1 69 3.48e-14 71.2
Msa1386860 AT4G37435.1 45.070 71 38 1 1 71 1 70 3.57e-14 70.5
Msa1386860 AT4G37940.1 45.070 71 37 1 1 71 1 69 4.23e-14 71.2
Msa1386860 AT1G69120.2 43.038 79 44 1 1 79 1 78 4.54e-14 71.2
Msa1386860 AT2G22540.2 52.830 53 25 0 1 53 1 53 5.38e-14 70.9
Msa1386860 AT1G69120.1 43.038 79 44 1 1 79 1 78 6.27e-14 71.2
Msa1386860 AT5G51870.1 45.333 75 39 1 1 75 1 73 7.81e-14 70.1
Msa1386860 AT5G51870.5 34.932 146 72 6 1 132 24 160 8.91e-14 69.7
Msa1386860 AT3G58780.2 52.830 53 25 0 1 53 16 68 9.33e-14 70.5
Msa1386860 AT3G58780.1 52.830 53 25 0 1 53 16 68 1.14e-13 70.1
Msa1386860 AT2G42830.2 52.830 53 25 0 1 53 16 68 1.15e-13 70.1
Msa1386860 AT2G42830.1 52.830 53 25 0 1 53 16 68 1.17e-13 70.1
Msa1386860 AT1G71692.1 42.500 80 45 1 1 80 1 79 1.19e-13 69.3
Msa1386860 AT3G58780.5 52.830 53 25 0 1 53 22 74 1.25e-13 70.1
Msa1386860 AT3G54340.1 29.008 131 90 2 1 131 1 128 1.34e-13 69.7
Msa1386860 AT5G51870.4 45.333 75 39 1 1 75 1 73 1.36e-13 69.3
Msa1386860 AT5G10140.4 32.000 175 82 9 1 163 1 150 1.52e-13 68.6
Msa1386860 AT4G09960.4 29.878 164 96 6 1 157 96 247 1.55e-13 70.9
Msa1386860 AT5G51870.3 45.333 75 39 1 1 75 11 83 1.59e-13 69.3
Msa1386860 AT3G58780.3 52.830 53 25 0 1 53 16 68 1.87e-13 70.1
Msa1386860 AT4G18960.1 53.846 52 24 0 2 53 18 69 1.98e-13 69.7
Msa1386860 AT2G22630.1 43.750 80 41 2 1 78 1 78 2.65e-13 68.9
Msa1386860 AT2G22630.2 43.750 80 41 2 1 78 1 78 2.65e-13 68.9
Msa1386860 AT3G58780.4 52.830 53 25 0 1 53 16 68 2.68e-13 69.7
Msa1386860 AT5G20240.2 50.943 53 26 0 1 53 1 53 2.98e-13 67.0
Msa1386860 AT5G60910.1 34.351 131 77 4 1 123 1 130 3.04e-13 68.9
Msa1386860 AT5G10140.1 30.573 157 78 6 1 145 1 138 3.46e-13 67.8
Msa1386860 AT5G10140.2 52.830 53 25 0 1 53 1 53 3.98e-13 67.0
Msa1386860 AT5G62165.14 40.789 76 43 1 1 76 1 74 3.98e-13 65.5
Msa1386860 AT5G20240.1 50.943 53 26 0 1 53 1 53 4.20e-13 67.8
Msa1386860 AT5G10140.3 52.830 53 25 0 1 53 1 53 5.10e-13 67.0
Msa1386860 AT3G30260.1 40.845 71 41 1 1 71 1 70 5.17e-13 68.6
Msa1386860 AT2G45650.1 52.830 53 25 0 1 53 1 53 8.03e-13 67.8
Msa1386860 AT5G62165.3 30.247 162 99 4 1 155 1 155 1.17e-12 66.6
Msa1386860 AT5G62165.1 30.247 162 99 4 1 155 1 155 1.17e-12 66.6
Msa1386860 AT5G62165.2 30.247 162 99 4 1 155 1 155 1.17e-12 66.6
Msa1386860 AT4G22950.3 30.435 161 99 5 1 155 1 154 1.67e-12 66.2
Msa1386860 AT4G22950.1 30.435 161 99 5 1 155 1 154 1.67e-12 66.2
Msa1386860 AT4G11880.4 30.921 152 91 5 1 145 1 145 1.99e-12 66.2
Msa1386860 AT4G11880.1 30.921 152 91 5 1 145 1 145 1.99e-12 66.2
Msa1386860 AT5G62165.17 30.247 162 99 4 1 155 1 155 2.08e-12 64.7
Msa1386860 AT5G62165.12 30.247 162 99 4 1 155 1 155 2.08e-12 64.7
Msa1386860 AT5G62165.11 30.247 162 99 4 1 155 1 155 2.08e-12 64.7
Msa1386860 AT1G24260.3 50.943 53 26 0 1 53 1 53 2.13e-12 66.2
Msa1386860 AT1G24260.2 50.943 53 26 0 1 53 1 53 2.69e-12 66.2
Msa1386860 AT1G24260.1 50.943 53 26 0 1 53 1 53 2.71e-12 66.2
Msa1386860 AT5G62165.6 40.789 76 43 1 1 76 1 74 2.77e-12 64.3
Msa1386860 AT5G62165.9 40.789 76 43 1 1 76 1 74 2.77e-12 64.3
Msa1386860 AT5G62165.7 40.789 76 43 1 1 76 1 74 2.77e-12 64.3
Msa1386860 AT5G62165.8 40.789 76 43 1 1 76 1 74 2.77e-12 64.3
Msa1386860 AT5G62165.13 40.789 76 43 1 1 76 1 74 2.77e-12 64.3
Msa1386860 AT5G15800.1 50.943 53 26 0 1 53 1 53 2.96e-12 66.2
Msa1386860 AT5G15800.2 50.943 53 26 0 1 53 1 53 3.83e-12 65.9
Msa1386860 AT5G62165.10 40.789 76 43 1 1 76 1 74 4.02e-12 64.7
Msa1386860 AT5G62165.4 40.789 76 43 1 1 76 1 74 4.02e-12 64.7
Msa1386860 AT3G02310.2 50.943 53 26 0 1 53 1 53 5.06e-12 64.7
Msa1386860 AT4G22950.2 30.435 161 99 5 1 155 1 154 5.07e-12 63.9
Msa1386860 AT5G51860.2 42.466 73 40 1 1 73 1 71 5.81e-12 64.3
Msa1386860 AT3G02310.1 50.943 53 26 0 1 53 1 53 5.92e-12 65.5
Msa1386860 AT5G51860.1 42.466 73 40 1 1 73 1 71 6.01e-12 64.7
Msa1386860 AT5G65050.2 50.943 53 26 0 1 53 1 53 8.21e-12 63.5
Msa1386860 AT2G34440.1 26.415 159 101 3 1 153 1 149 1.21e-11 62.8
Msa1386860 AT5G65050.3 43.662 71 39 1 1 71 1 70 1.70e-11 63.2
Msa1386860 AT5G65050.1 43.662 71 39 1 1 71 1 70 1.83e-11 62.8
Msa1386860 AT5G65060.2 30.519 154 89 3 1 154 1 136 1.85e-11 62.8
Msa1386860 AT5G65050.5 43.662 71 39 1 1 71 1 70 2.02e-11 62.8
Msa1386860 AT5G65070.4 47.170 53 28 0 1 53 1 53 2.03e-11 61.6
Msa1386860 AT5G65070.1 47.170 53 28 0 1 53 1 53 3.59e-11 62.0
Msa1386860 AT3G66656.1 46.296 54 29 0 1 54 1 54 3.59e-11 61.6
Msa1386860 AT1G01530.1 48.148 54 28 0 1 54 6 59 4.11e-11 62.8
Msa1386860 AT4G36590.1 43.662 71 38 1 2 72 7 75 5.68e-11 62.4
Msa1386860 AT5G65070.5 47.170 53 28 0 1 53 1 53 5.88e-11 61.6
Msa1386860 AT5G65070.3 47.170 53 28 0 1 53 1 53 5.98e-11 62.0
Msa1386860 AT5G65070.2 47.170 53 28 0 1 53 1 53 6.62e-11 62.0

Find 77 sgRNAs with CRISPR-Local

Find 157 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTCTCCACATGTCACTATTT+TGG 0.102186 tig0020872:+58305 Msa1386860:CDS
TAAATTGAAGATAAAGAAAT+TGG 0.209657 tig0020872:+55869 Msa1386860:CDS
GTTCTAATTGAGCAGGAAAA+TGG 0.237423 tig0020872:+56538 Msa1386860:intron
CCAAGCTATTCTGGTTATAC+TGG 0.263985 tig0020872:+58258 Msa1386860:CDS
TGCACATTTAAGTAAGGATT+TGG 0.267580 tig0020872:-58366 None:intergenic
CCAAAATCACTATTTGAAAA+TGG 0.281681 tig0020872:+58507 Msa1386860:CDS
TTGAAAATGGACACCAGTTC+TGG 0.282537 tig0020872:+58520 Msa1386860:CDS
AACCAAAGTTTATGTCATTC+AGG 0.285801 tig0020872:+57966 Msa1386860:CDS
CCCTTTCTTGTGGGCTAAGT+TGG 0.290811 tig0020872:-56439 None:intergenic
TTGTAGACCAGACATCATTA+AGG 0.306825 tig0020872:-57882 None:intergenic
ATTTCACTCCCAAGCTATTC+TGG 0.316741 tig0020872:+58249 Msa1386860:CDS
GGTACCTTGAATGAGTTAAA+TGG 0.322272 tig0020872:+58339 Msa1386860:CDS
GCCCACAAGAAAGGGCTAAA+AGG 0.341342 tig0020872:+56447 Msa1386860:CDS
CGCATTCAAGTGACATTAGT+TGG 0.358372 tig0020872:-57745 None:intergenic
TGTAAAGATACAAGATTTCC+TGG 0.370430 tig0020872:+56725 Msa1386860:CDS
CCTTTCTTGTGGGCTAAGTT+GGG 0.379085 tig0020872:-56438 None:intergenic
AGGAAAGCCTACGTTATTAC+AGG 0.394098 tig0020872:+56010 Msa1386860:CDS
CACTGTTGCACATTTAAGTA+AGG 0.404816 tig0020872:-58372 None:intergenic
AAGGGCAAAGTCATCCCTTC+TGG 0.405073 tig0020872:-57863 None:intergenic
TCTCAGTTATTGGAGTAATC+TGG 0.414099 tig0020872:+57497 Msa1386860:intron
ACTTTGCCCTTAATGATGTC+TGG 0.425266 tig0020872:+57875 Msa1386860:CDS
ATCATGATCCAATTTCTTGA+AGG 0.431577 tig0020872:-56703 None:intergenic
CCCAACTTAGCCCACAAGAA+AGG 0.433847 tig0020872:+56438 Msa1386860:CDS
CTTGGAACATCTTAGACAAA+TGG 0.437086 tig0020872:+57533 Msa1386860:CDS
GTTCACTAGGTAACATCAAC+TGG 0.439721 tig0020872:-57946 None:intergenic
TGTAGACCAGACATCATTAA+GGG 0.442310 tig0020872:-57881 None:intergenic
TTGATTTGCTATTGATTGGT+TGG 0.442413 tig0020872:-58446 None:intergenic
CACCTGAATGACATAAACTT+TGG 0.443645 tig0020872:-57968 None:intergenic
CAATCGATTGTCTATACAAA+AGG 0.445203 tig0020872:+57578 Msa1386860:CDS
TTATTTGATTTGCTATTGAT+TGG 0.458324 tig0020872:-58450 None:intergenic
TTCACTTCCTCAAGATCTTG+TGG 0.459942 tig0020872:-58417 None:intergenic
GATCATAATTGTTATTAACT+TGG 0.470376 tig0020872:-58482 None:intergenic
AAGAATCTCAACCTTTGTCT+TGG 0.471192 tig0020872:+57903 Msa1386860:CDS
TACAAGATTTCCTGGGTTCA+AGG 0.476006 tig0020872:+56733 Msa1386860:CDS
ATTTCCATTTAACTCATTCA+AGG 0.477610 tig0020872:-58343 None:intergenic
TTATACTGGGAACTGCAAAC+TGG 0.478109 tig0020872:+58272 Msa1386860:CDS
TGGGAACTGCAAACTGGAGG+TGG 0.480619 tig0020872:+58278 Msa1386860:CDS
CAGTATAACCAGAATAGCTT+GGG 0.488834 tig0020872:-58257 None:intergenic
TCCTCAAGATCTTGTGGAGG+AGG 0.493605 tig0020872:-58411 None:intergenic
CCAACTTAGCCCACAAGAAA+GGG 0.507371 tig0020872:+56439 Msa1386860:CDS
CCAGTATAACCAGAATAGCT+TGG 0.510057 tig0020872:-58258 None:intergenic
CATTTGTAGAATTCCAGAAC+TGG 0.522572 tig0020872:-58533 None:intergenic
GTCACTTGAATGCGTTAACC+AGG 0.524046 tig0020872:+57754 Msa1386860:CDS
CAGTTCTGGAATTCTACAAA+TGG 0.526268 tig0020872:+58534 Msa1386860:CDS
TCTGGAGAAGATCAGTAACT+TGG 0.527978 tig0020872:+57515 Msa1386860:CDS
ACTTATTCGAAGCGGAAAAG+TGG 0.527998 tig0020872:+55915 Msa1386860:CDS
AAAACTACATGCTTACCCTA+TGG 0.529353 tig0020872:-58593 None:intergenic
ATTGCTATGTATAACGAGAA+TGG 0.533675 tig0020872:+58567 Msa1386860:CDS
CAAGCTATTCTGGTTATACT+GGG 0.535943 tig0020872:+58259 Msa1386860:CDS
GACAAATGGAAGACTCACTC+AGG 0.540615 tig0020872:+57547 Msa1386860:CDS
CCTTTGTCTTGGCTTCTAAG+TGG 0.542337 tig0020872:+57914 Msa1386860:CDS
CCCTCCTCCACAAGATCTTG+AGG 0.562025 tig0020872:+58410 Msa1386860:CDS
ATAACGAGAATGGTTACCAT+AGG 0.564017 tig0020872:+58577 Msa1386860:CDS
ATTGATTCATAGTTACCAGA+AGG 0.565773 tig0020872:+57848 Msa1386860:intron
CTGTTGGAGAAAACATGAGA+AGG 0.576749 tig0020872:-55989 None:intergenic
AATTCTACAAATGGCTCTTG+TGG 0.581991 tig0020872:+58543 Msa1386860:CDS
CTTATTCGAAGCGGAAAAGT+GGG 0.585417 tig0020872:+55916 Msa1386860:CDS
CAATCACTTACCTTGAACCC+AGG 0.589742 tig0020872:-56743 None:intergenic
CGTTCTCCCTGTAATAACGT+AGG 0.597125 tig0020872:-56017 None:intergenic
GTAAAGATACAAGATTTCCT+GGG 0.597719 tig0020872:+56726 Msa1386860:CDS
TAACGAGAATGGTTACCATA+GGG 0.597927 tig0020872:+58578 Msa1386860:CDS
CCTCAAGATCTTGTGGAGGA+GGG 0.600496 tig0020872:-58410 None:intergenic
GACATAAACTTTGGTTCACT+AGG 0.613858 tig0020872:-57959 None:intergenic
CAATAGCAAATCAAATAACG+TGG 0.614557 tig0020872:+58455 Msa1386860:CDS
ACTTCCTCAAGATCTTGTGG+AGG 0.620137 tig0020872:-58414 None:intergenic
GGGAACTGCAAACTGGAGGT+GGG 0.623032 tig0020872:+58279 Msa1386860:CDS
AATAACGTAGGCTTTCCTGT+TGG 0.623309 tig0020872:-56005 None:intergenic
TTGATTCATAGTTACCAGAA+GGG 0.636080 tig0020872:+57849 Msa1386860:intron
ATAGCTTGGGAGTGAAATGT+CGG 0.637360 tig0020872:-58244 None:intergenic
TACTGGGAACTGCAAACTGG+AGG 0.648889 tig0020872:+58275 Msa1386860:CDS
TGACCCAAAATAGTGACATG+TGG 0.657881 tig0020872:-58309 None:intergenic
GCTTCGAATAAGTCACATGT+CGG 0.667125 tig0020872:-55905 None:intergenic
ACAAATGGAAGACTCACTCA+GGG 0.670210 tig0020872:+57548 Msa1386860:CDS
GGAAAGCCTACGTTATTACA+GGG 0.679926 tig0020872:+56011 Msa1386860:CDS
GACATGTGACTTATTCGAAG+CGG 0.683369 tig0020872:+55907 Msa1386860:CDS
CCACTTAGAAGCCAAGACAA+AGG 0.694579 tig0020872:-57914 None:intergenic
CGTTATTACAGGGAGAACGA+AGG 0.718082 tig0020872:+56021 Msa1386860:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! GTTCAAATATTTTAAAAAAA+TGG + tig0020872:56223-56242 Msa1386860:intron 10.0%
!!! GTATATCTATTTTTTTTTTA+AGG - tig0020872:56286-56305 None:intergenic 10.0%
!! ATAATAATAGATAGAAAAAA+GGG - tig0020872:57798-57817 None:intergenic 10.0%
!! TATAATAATAGATAGAAAAA+AGG - tig0020872:57799-57818 None:intergenic 10.0%
!! TAAATTGAAGATAAAGAAAT+TGG + tig0020872:55869-55888 Msa1386860:CDS 15.0%
!! AAAGTAATATATGAATTACA+AGG - tig0020872:56593-56612 None:intergenic 15.0%
!! TTTATTCACAAATTATCAAA+TGG + tig0020872:56863-56882 Msa1386860:intron 15.0%
!! TTATTCACAAATTATCAAAT+GGG + tig0020872:56864-56883 Msa1386860:intron 15.0%
!!! TATTGATATTTTGATTTCAT+CGG + tig0020872:57702-57721 Msa1386860:intron 15.0%
!!! ATTTTTGAAATAATCTTATC+CGG + tig0020872:57819-57838 Msa1386860:intron 15.0%
!!! ACTTATCATTTTAATCTCAA+AGG + tig0020872:56195-56214 Msa1386860:intron 20.0%
!!! ATTTTATTTTTCCAGCAATA+TGG + tig0020872:56394-56413 Msa1386860:intron 20.0%
!!! TTTGGTCTTGTTTAAAATTT+AGG + tig0020872:56668-56687 Msa1386860:intron 20.0%
!! AATCTTCAAAATCATTTACA+AGG - tig0020872:56793-56812 None:intergenic 20.0%
!! TGCAATATGAAAATTAAAAC+AGG - tig0020872:56820-56839 None:intergenic 20.0%
!! CGAAAAATTTAAATTGAACA+AGG - tig0020872:57194-57213 None:intergenic 20.0%
!! TGATAGTATTTGACATAATT+TGG - tig0020872:57433-57452 None:intergenic 20.0%
!! TGTCAAATACTATCATAATT+TGG + tig0020872:57438-57457 Msa1386860:intron 20.0%
!! GTCAAATACTATCATAATTT+GGG + tig0020872:57439-57458 Msa1386860:intron 20.0%
!! ATATGTAATATCTCAGTTAT+TGG + tig0020872:57487-57506 Msa1386860:intron 20.0%
!! TCTATACAAAAGGTAATATT+TGG + tig0020872:57588-57607 Msa1386860:intron 20.0%
!! AAAATACACTATTATTTACC+TGG - tig0020872:57775-57794 None:intergenic 20.0%
!!! TTTTTGAAATAATCTTATCC+GGG + tig0020872:57820-57839 Msa1386860:intron 20.0%
!!! TTATTTGATTTGCTATTGAT+TGG - tig0020872:58453-58472 None:intergenic 20.0%
!!! GATCATAATTGTTATTAACT+TGG - tig0020872:58485-58504 None:intergenic 20.0%
! CGAAGGTAATGTAATAATAA+CGG + tig0020872:56038-56057 Msa1386860:intron 25.0%
!! TTTCTCATTTTATCACAAGA+AGG - tig0020872:56113-56132 None:intergenic 25.0%
! CTTGTGATAAAATGAGAAAA+TGG + tig0020872:56114-56133 Msa1386860:intron 25.0%
!!! ATCATTTTAATCTCAAAGGA+CGG + tig0020872:56199-56218 Msa1386860:intron 25.0%
!!! ACTTTTACACACTTTAAAGA+TGG - tig0020872:56325-56344 None:intergenic 25.0%
! TCAGTCATTGCTTAAAAATT+TGG + tig0020872:56650-56669 Msa1386860:intron 25.0%
! GAAAAAAACCTTCAAGAAAT+TGG + tig0020872:56695-56714 Msa1386860:CDS 25.0%
! ATGAATAATAGTTCAACAAG+AGG - tig0020872:57088-57107 None:intergenic 25.0%
! TACACACTCATAATTAATAC+AGG - tig0020872:57159-57178 None:intergenic 25.0%
!!! CGACAAACACATTTTTTTTT+GGG - tig0020872:57366-57385 None:intergenic 25.0%
!!! TCGACAAACACATTTTTTTT+TGG - tig0020872:57367-57386 None:intergenic 25.0%
!! AGTTTGTACCAAAAAAGTAT+AGG - tig0020872:57392-57411 None:intergenic 25.0%
!! TAATCACAACGATTTTCTAA+GGG - tig0020872:57663-57682 None:intergenic 25.0%
!! TTAATCACAACGATTTTCTA+AGG - tig0020872:57664-57683 None:intergenic 25.0%
!! AAAGAGCTTTATAACAAAAG+TGG + tig0020872:58017-58036 Msa1386860:intron 25.0%
! ATTTCCATTTAACTCATTCA+AGG - tig0020872:58346-58365 None:intergenic 25.0%
!! CCATTTTCAAATAGTGATTT+TGG - tig0020872:58510-58529 None:intergenic 25.0%
! CCAAAATCACTATTTGAAAA+TGG + tig0020872:58507-58526 Msa1386860:CDS 25.0%
TTAAAGTGTGTAAAAGTCGA+AGG + tig0020872:56328-56347 Msa1386860:intron 30.0%
!! TTATTTTTCCAGCAATATGG+AGG + tig0020872:56397-56416 Msa1386860:intron 30.0%
! TTTTCTTGTTCTAATTGAGC+AGG + tig0020872:56531-56550 Msa1386860:intron 30.0%
ATCATGATCCAATTTCTTGA+AGG - tig0020872:56706-56725 None:intergenic 30.0%
TGTAAAGATACAAGATTTCC+TGG + tig0020872:56725-56744 Msa1386860:CDS 30.0%
GTAAAGATACAAGATTTCCT+GGG + tig0020872:56726-56745 Msa1386860:CDS 30.0%
!! ATTGCTTTAGAATCTTAAGC+TGG + tig0020872:56893-56912 Msa1386860:intron 30.0%
AGAATCTTAAGCTGGTAATT+AGG + tig0020872:56901-56920 Msa1386860:intron 30.0%
CAATTGAACTCATAACACTT+TGG - tig0020872:56930-56949 None:intergenic 30.0%
ATACAAAATTGGTAAGGTAG+AGG - tig0020872:57115-57134 None:intergenic 30.0%
AAGCACATACAAAATTGGTA+AGG - tig0020872:57121-57140 None:intergenic 30.0%
TTGCAAAGCACATACAAAAT+TGG - tig0020872:57126-57145 None:intergenic 30.0%
!!! TTTTTTGGGTGGTGTTTATT+TGG - tig0020872:57352-57371 None:intergenic 30.0%
!!! CAAACACATTTTTTTTTGGG+TGG - tig0020872:57363-57382 None:intergenic 30.0%
!!! TGTCGAAACCTATACTTTTT+TGG + tig0020872:57381-57400 Msa1386860:intron 30.0%
CAATCGATTGTCTATACAAA+AGG + tig0020872:57578-57597 Msa1386860:CDS 30.0%
! AGGTAATATTTGGTTTCATG+CGG + tig0020872:57598-57617 Msa1386860:intron 30.0%
! AATCACAACGATTTTCTAAG+GGG - tig0020872:57662-57681 None:intergenic 30.0%
ACTATGAATCAATATCATCC+CGG - tig0020872:57841-57860 None:intergenic 30.0%
! ATTGATTCATAGTTACCAGA+AGG + tig0020872:57848-57867 Msa1386860:intron 30.0%
TTGATTCATAGTTACCAGAA+GGG + tig0020872:57849-57868 Msa1386860:intron 30.0%
AACCAAAGTTTATGTCATTC+AGG + tig0020872:57966-57985 Msa1386860:CDS 30.0%
TGCACATTTAAGTAAGGATT+TGG - tig0020872:58369-58388 None:intergenic 30.0%
!! TTGATTTGCTATTGATTGGT+TGG - tig0020872:58449-58468 None:intergenic 30.0%
CAATAGCAAATCAAATAACG+TGG + tig0020872:58455-58474 Msa1386860:CDS 30.0%
ATTGCTATGTATAACGAGAA+TGG + tig0020872:58567-58586 Msa1386860:CDS 30.0%
GTTCTAATTGAGCAGGAAAA+TGG + tig0020872:56538-56557 Msa1386860:intron 35.0%
! AGATTCTCACTTGATGACTT+AGG - tig0020872:56965-56984 None:intergenic 35.0%
AAGTGAGAATCTTACAAGCT+CGG + tig0020872:56973-56992 Msa1386860:intron 35.0%
!! GTTTATTTGGTGTGAGCTAA+AGG - tig0020872:57339-57358 None:intergenic 35.0%
TCTCAGTTATTGGAGTAATC+TGG + tig0020872:57497-57516 Msa1386860:intron 35.0%
CTTGGAACATCTTAGACAAA+TGG + tig0020872:57533-57552 Msa1386860:CDS 35.0%
!!! GATATTTTGATTTCATCGGC+AGG + tig0020872:57706-57725 Msa1386860:intron 35.0%
TGTAGACCAGACATCATTAA+GGG - tig0020872:57884-57903 None:intergenic 35.0%
TTGTAGACCAGACATCATTA+AGG - tig0020872:57885-57904 None:intergenic 35.0%
AAGAATCTCAACCTTTGTCT+TGG + tig0020872:57903-57922 Msa1386860:CDS 35.0%
! GACATAAACTTTGGTTCACT+AGG - tig0020872:57962-57981 None:intergenic 35.0%
CACCTGAATGACATAAACTT+TGG - tig0020872:57971-57990 None:intergenic 35.0%
CAGTATAACCAGAATAGCTT+GGG - tig0020872:58260-58279 None:intergenic 35.0%
! CAAGCTATTCTGGTTATACT+GGG + tig0020872:58259-58278 Msa1386860:CDS 35.0%
! TCTCCACATGTCACTATTTT+GGG + tig0020872:58306-58325 Msa1386860:CDS 35.0%
GGTACCTTGAATGAGTTAAA+TGG + tig0020872:58339-58358 Msa1386860:CDS 35.0%
CACTGTTGCACATTTAAGTA+AGG - tig0020872:58375-58394 None:intergenic 35.0%
CATTTGTAGAATTCCAGAAC+TGG - tig0020872:58536-58555 None:intergenic 35.0%
!! CAGTTCTGGAATTCTACAAA+TGG + tig0020872:58534-58553 Msa1386860:CDS 35.0%
AATTCTACAAATGGCTCTTG+TGG + tig0020872:58543-58562 Msa1386860:CDS 35.0%
ATAACGAGAATGGTTACCAT+AGG + tig0020872:58577-58596 Msa1386860:CDS 35.0%
TAACGAGAATGGTTACCATA+GGG + tig0020872:58578-58597 Msa1386860:CDS 35.0%
GCTTCGAATAAGTCACATGT+CGG - tig0020872:55908-55927 None:intergenic 40.0%
GACATGTGACTTATTCGAAG+CGG + tig0020872:55907-55926 Msa1386860:CDS 40.0%
ACTTATTCGAAGCGGAAAAG+TGG + tig0020872:55915-55934 Msa1386860:CDS 40.0%
CTTATTCGAAGCGGAAAAGT+GGG + tig0020872:55916-55935 Msa1386860:CDS 40.0%
CTGTTGGAGAAAACATGAGA+AGG - tig0020872:55992-56011 None:intergenic 40.0%
! CTTCTCATGTTTTCTCCAAC+AGG + tig0020872:55990-56009 Msa1386860:CDS 40.0%
AATAACGTAGGCTTTCCTGT+TGG - tig0020872:56008-56027 None:intergenic 40.0%
AGGAAAGCCTACGTTATTAC+AGG + tig0020872:56010-56029 Msa1386860:CDS 40.0%
GGAAAGCCTACGTTATTACA+GGG + tig0020872:56011-56030 Msa1386860:CDS 40.0%
AATGATCTCCTCCATATTGC+TGG - tig0020872:56408-56427 None:intergenic 40.0%
! ACCTTTTAGCCCTTTCTTGT+GGG - tig0020872:56451-56470 None:intergenic 40.0%
! AACCTTTTAGCCCTTTCTTG+TGG - tig0020872:56452-56471 None:intergenic 40.0%
TACAAGATTTCCTGGGTTCA+AGG + tig0020872:56733-56752 Msa1386860:CDS 40.0%
ATATTGCAGCAGTCAAACAG+TGG + tig0020872:56832-56851 Msa1386860:intron 40.0%
! TGAGTTCAATTGCCATTTGC+AGG + tig0020872:56938-56957 Msa1386860:intron 40.0%
CAGAAATACAACAGAGACTG+AGG + tig0020872:57000-57019 Msa1386860:intron 40.0%
TCTGGAGAAGATCAGTAACT+TGG + tig0020872:57515-57534 Msa1386860:CDS 40.0%
ACAAATGGAAGACTCACTCA+GGG + tig0020872:57548-57567 Msa1386860:CDS 40.0%
!! ACAACGATTTTCTAAGGGGA+CGG - tig0020872:57658-57677 None:intergenic 40.0%
CGCATTCAAGTGACATTAGT+TGG - tig0020872:57748-57767 None:intergenic 40.0%
ACTTTGCCCTTAATGATGTC+TGG + tig0020872:57875-57894 Msa1386860:CDS 40.0%
GTTCACTAGGTAACATCAAC+TGG - tig0020872:57949-57968 None:intergenic 40.0%
ATAGCTTGGGAGTGAAATGT+CGG - tig0020872:58247-58266 None:intergenic 40.0%
ATTTCACTCCCAAGCTATTC+TGG + tig0020872:58249-58268 Msa1386860:CDS 40.0%
CCAGTATAACCAGAATAGCT+TGG - tig0020872:58261-58280 None:intergenic 40.0%
! CCAAGCTATTCTGGTTATAC+TGG + tig0020872:58258-58277 Msa1386860:CDS 40.0%
TTATACTGGGAACTGCAAAC+TGG + tig0020872:58272-58291 Msa1386860:CDS 40.0%
! CTCTCCACATGTCACTATTT+TGG + tig0020872:58305-58324 Msa1386860:CDS 40.0%
TGACCCAAAATAGTGACATG+TGG - tig0020872:58312-58331 None:intergenic 40.0%
!! ACATGTCACTATTTTGGGTC+AGG + tig0020872:58311-58330 Msa1386860:CDS 40.0%
!! CATGTCACTATTTTGGGTCA+GGG + tig0020872:58312-58331 Msa1386860:CDS 40.0%
TTCACTTCCTCAAGATCTTG+TGG - tig0020872:58420-58439 None:intergenic 40.0%
TTGAAAATGGACACCAGTTC+TGG + tig0020872:58520-58539 Msa1386860:CDS 40.0%
CGTTCTCCCTGTAATAACGT+AGG - tig0020872:56020-56039 None:intergenic 45.0%
CGTTATTACAGGGAGAACGA+AGG + tig0020872:56021-56040 Msa1386860:CDS 45.0%
! CCTTTCTTGTGGGCTAAGTT+GGG - tig0020872:56441-56460 None:intergenic 45.0%
CCAACTTAGCCCACAAGAAA+GGG + tig0020872:56439-56458 Msa1386860:CDS 45.0%
CAATCACTTACCTTGAACCC+AGG - tig0020872:56746-56765 None:intergenic 45.0%
GATGACTTAGGTCCTGCAAA+TGG - tig0020872:56953-56972 None:intergenic 45.0%
!!! AGGTGCAACTTTGGAGCTTT+TGG + tig0020872:57020-57039 Msa1386860:intron 45.0%
! TTGGTGTGAGCTAAAGGCAT+GGG - tig0020872:57333-57352 None:intergenic 45.0%
! TTTGGTGTGAGCTAAAGGCA+TGG - tig0020872:57334-57353 None:intergenic 45.0%
GACAAATGGAAGACTCACTC+AGG + tig0020872:57547-57566 Msa1386860:CDS 45.0%
!! CAACGATTTTCTAAGGGGAC+GGG - tig0020872:57657-57676 None:intergenic 45.0%
GTCACTTGAATGCGTTAACC+AGG + tig0020872:57754-57773 Msa1386860:CDS 45.0%
CCACTTAGAAGCCAAGACAA+AGG - tig0020872:57917-57936 None:intergenic 45.0%
CCTTTGTCTTGGCTTCTAAG+TGG + tig0020872:57914-57933 Msa1386860:CDS 45.0%
ACTTCCTCAAGATCTTGTGG+AGG - tig0020872:58417-58436 None:intergenic 45.0%
!! AATAAAAATAAATATTTAGT+GGG - tig0020872:56486-56505 None:intergenic 5.0%
!! AAATAAAAATAAATATTTAG+TGG - tig0020872:56487-56506 None:intergenic 5.0%
CCCAACTTAGCCCACAAGAA+AGG + tig0020872:56438-56457 Msa1386860:CDS 50.0%
! CCCTTTCTTGTGGGCTAAGT+TGG - tig0020872:56442-56461 None:intergenic 50.0%
GCCCACAAGAAAGGGCTAAA+AGG + tig0020872:56447-56466 Msa1386860:CDS 50.0%
TTGCAGCAGTCAAACAGTGG+AGG + tig0020872:56835-56854 Msa1386860:intron 50.0%
CAGAGACTGAGGTGCAACTT+TGG + tig0020872:57011-57030 Msa1386860:intron 50.0%
! TGGTGTGAGCTAAAGGCATG+GGG - tig0020872:57332-57351 None:intergenic 50.0%
AAGGGCAAAGTCATCCCTTC+TGG - tig0020872:57866-57885 None:intergenic 50.0%
TACTGGGAACTGCAAACTGG+AGG + tig0020872:58275-58294 Msa1386860:CDS 50.0%
!! GTCACTATTTTGGGTCAGGG+AGG + tig0020872:58315-58334 Msa1386860:CDS 50.0%
!! ACTATTTTGGGTCAGGGAGG+TGG + tig0020872:58318-58337 Msa1386860:CDS 50.0%
CCTCAAGATCTTGTGGAGGA+GGG - tig0020872:58413-58432 None:intergenic 50.0%
TCCTCAAGATCTTGTGGAGG+AGG - tig0020872:58414-58433 None:intergenic 50.0%
! TTCTAAGGGGACGGGAACGA+TGG - tig0020872:57649-57668 None:intergenic 55.0%
TGGGAACTGCAAACTGGAGG+TGG + tig0020872:58278-58297 Msa1386860:CDS 55.0%
GGGAACTGCAAACTGGAGGT+GGG + tig0020872:58279-58298 Msa1386860:CDS 55.0%
CCCTCCTCCACAAGATCTTG+AGG + tig0020872:58410-58429 Msa1386860:CDS 55.0%
Chromosome Type Strat End Strand Name
tig0020872 gene 55858 58611 55858 ID=Msa1386860;Name=Msa1386860
tig0020872 mRNA 55858 58611 55858 ID=Msa1386860-mRNA-1;Parent=Msa1386860;Name=Msa1386860-mRNA-1;_AED=0.08;_eAED=0.09;_QI=0|0|0|1|1|1|8|0|333
tig0020872 exon 55858 56042 55858 ID=Msa1386860-mRNA-1:exon:10363;Parent=Msa1386860-mRNA-1
tig0020872 exon 56409 56468 56409 ID=Msa1386860-mRNA-1:exon:10364;Parent=Msa1386860-mRNA-1
tig0020872 exon 56553 56571 56553 ID=Msa1386860-mRNA-1:exon:10365;Parent=Msa1386860-mRNA-1
tig0020872 exon 56690 56754 56690 ID=Msa1386860-mRNA-1:exon:10366;Parent=Msa1386860-mRNA-1
tig0020872 exon 57503 57599 57503 ID=Msa1386860-mRNA-1:exon:10367;Parent=Msa1386860-mRNA-1
tig0020872 exon 57728 57775 57728 ID=Msa1386860-mRNA-1:exon:10368;Parent=Msa1386860-mRNA-1
tig0020872 exon 57860 57987 57860 ID=Msa1386860-mRNA-1:exon:10369;Parent=Msa1386860-mRNA-1
tig0020872 exon 58212 58611 58212 ID=Msa1386860-mRNA-1:exon:10370;Parent=Msa1386860-mRNA-1
tig0020872 CDS 55858 56042 55858 ID=Msa1386860-mRNA-1:cds;Parent=Msa1386860-mRNA-1
tig0020872 CDS 56409 56468 56409 ID=Msa1386860-mRNA-1:cds;Parent=Msa1386860-mRNA-1
tig0020872 CDS 56553 56571 56553 ID=Msa1386860-mRNA-1:cds;Parent=Msa1386860-mRNA-1
tig0020872 CDS 56690 56754 56690 ID=Msa1386860-mRNA-1:cds;Parent=Msa1386860-mRNA-1
tig0020872 CDS 57503 57599 57503 ID=Msa1386860-mRNA-1:cds;Parent=Msa1386860-mRNA-1
tig0020872 CDS 57728 57775 57728 ID=Msa1386860-mRNA-1:cds;Parent=Msa1386860-mRNA-1
tig0020872 CDS 57860 57987 57860 ID=Msa1386860-mRNA-1:cds;Parent=Msa1386860-mRNA-1
tig0020872 CDS 58212 58611 58212 ID=Msa1386860-mRNA-1:cds;Parent=Msa1386860-mRNA-1
Gene Sequence

>Msa1386860

ATGGGGAGAGTTAAATTGAAGATAAAGAAATTGGAGAGCACAAGCAACCGACATGTGACTTATTCGAAGCGGAAAAGTGGGATCTTGAAGAAAGCAAAAGAGTTATCAATTCTATGTGATATAGATATTCTCCTTCTCATGTTTTCTCCAACAGGAAAGCCTACGTTATTACAGGGAGAACGAAGCAATATGGAGGAGATCATTGCAAAATTTGCCCAACTTAGCCCACAAGAAAGGGCTAAAAGGAAAATGGAAAGTCTTGAAGCACTGAAAAAAACCTTCAAGAAATTGGATCATGATGTAAAGATACAAGATTTCCTGGGTTCAAGTTATTGGAGTAATCTGGAGAAGATCAGTAACTTGGAACATCTTAGACAAATGGAAGACTCACTCAGGGAATCAATCAATCGATTGTCTATACAAAAGGAAAATCTCGAAAAGCACCAACTAATGTCACTTGAATGCGTTAACCAGTTACCAGAAGGGATGACTTTGCCCTTAATGATGTCTGGTCTACAAGAATCTCAACCTTTGTCTTGGCTTCTAAGTGGTGATAATCACCAGTTGATGTTACCTAGTGAACCAAAGTTTATGTCATTCAGTGACAATGTCAATAGAGATGTTGAGTGCTCAACCGACATTTCACTCCCAAGCTATTCTGGTTATACTGGGAACTGCAAACTGGAGGTGGGAAGCTCTCCACATGTCACTATTTTGGGTCAGGGAGGTGGTACCTTGAATGAGTTAAATGGAAATCCAAATCCTTACTTAAATGTGCAACAGTGTCAGCAGTTTGCATACCCTCCTCCACAAGATCTTGAGGAAGTGAAACATCACCAACCAATCAATAGCAAATCAAATAACGTGGACTACCAAGTTAATAACAATTATGATCTGCCAAAATCACTATTTGAAAATGGACACCAGTTCTGGAATTCTACAAATGGCTCTTGTGGTATTGCTATGTATAACGAGAATGGTTACCATAGGGTAAGCATGTAG
Protein sequence

>Msa1386860

MGRVKLKIKKLESTSNRHVTYSKRKSGILKKAKELSILCDIDILLLMFSPTGKPTLLQGERSNMEEIIAKFAQLSPQERAKRKMESLEALKKTFKKLDHDVKIQDFLGSSYWSNLEKISNLEHLRQMEDSLRESINRLSIQKENLEKHQLMSLECVNQLPEGMTLPLMMSGLQESQPLSWLLSGDNHQLMLPSEPKFMSFSDNVNRDVECSTDISLPSYSGYTGNCKLEVGSSPHVTILGQGGGTLNELNGNPNPYLNVQQCQQFAYPPPQDLEEVKHHQPINSKSNNVDYQVNNNYDLPKSLFENGHQFWNSTNGSCGIAMYNENGYHRVSM*