AlfalfaGEDB | Msa1387360

M. sativa cultivar zhongmu-4 / Msa1387360

KEGG: K04371|K14512|K20535|K20536|K20537|K20600
GO: GO:0000165 GO:0000302 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004707 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006979 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0009636 GO:0009987 GO:0010035 GO:0010468 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019222 GO:0019538 GO:0023014 GO:0023052 GO:0035556 GO:0036211 GO:0042221 GO:0042493 GO:0042542 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0046677 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0060255 GO:0065007 GO:0071704 GO:0140096 GO:1901564 GO:1901700
PFAM: PF00069.28

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1387360	XP_003600359.1	81.793	368	33	1	1	334	1	368	0.0	615

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1387360	sp\|Q39027\|MPK7_ARATH	63.686	369	98	3	1	334	1	368	1.58e-163	463

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1387360	G7IV22	81.793	368	33	1	1	334	1	368	0.0	615

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1387360	MtrunA17_Chr3g0102571	72.356	416	33	2	1	334	1	416	0.0	593
Msa1387360	MtrunA17_Chr8g0378831	65.041	369	93	3	1	334	1	368	2.38e-172	483
Msa1387360	MtrunA17_Chr3g0102601	85.882	255	17	2	98	334	1	254	5.95e-158	442
Msa1387360	MtrunA17_Chr3g0141871	60.976	369	108	4	1	334	1	368	2.01e-157	445
Msa1387360	MtrunA17_Chr5g0397261	44.220	346	156	6	19	328	27	371	2.32e-102	305
Msa1387360	MtrunA17_Chr4g0045841	44.348	345	156	5	19	328	41	384	4.50e-102	305
Msa1387360	MtrunA17_Chr4g0030151	44.751	362	163	6	5	330	11	371	2.52e-101	303
Msa1387360	MtrunA17_Chr7g0248461	44.253	348	153	7	19	328	24	368	7.07e-101	301
Msa1387360	MtrunA17_Chr8g0387401	44.540	348	152	7	19	328	38	382	5.97e-100	300
Msa1387360	MtrunA17_Chr8g0348481	42.816	348	158	7	19	328	23	367	1.74e-99	298

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1387360	AT2G18170.1	63.686	369	98	3	1	334	1	368	1.60e-164	463
Msa1387360	AT1G10210.2	62.331	369	103	3	1	334	1	368	6.85e-159	449
Msa1387360	AT1G10210.3	62.331	369	103	3	1	334	1	368	6.85e-159	449
Msa1387360	AT1G10210.1	62.331	369	103	3	1	334	1	368	6.85e-159	449
Msa1387360	AT4G36450.1	62.155	362	103	5	1	329	1	361	2.89e-152	432
Msa1387360	AT1G59580.2	59.730	370	111	4	1	333	1	369	9.29e-149	424
Msa1387360	AT1G59580.1	59.730	370	111	4	1	333	1	369	9.29e-149	424
Msa1387360	AT1G07880.2	44.638	345	150	6	19	325	19	360	1.45e-102	306
Msa1387360	AT3G45640.1	44.847	359	157	7	7	327	12	367	9.77e-101	301
Msa1387360	AT4G01370.1	43.966	348	154	7	19	328	29	373	2.76e-100	300

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Find 62 sgRNAs with CRISPR-Local

Find 228 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAAGCATTGTTATACAATG+TGG	0.550208	tig0020883:-109659	Msa1387360:CDS
AAACTGATTATAAGTGTTCT+TGG	0.276779	tig0020883:-108416	Msa1387360:CDS
AAAGCATTGTTATACAATGT+GGG	0.563141	tig0020883:-109658	Msa1387360:CDS
AAGTGTGTCCCCTCTGTATA+AGG	0.363602	tig0020883:+108329	None:intergenic
AATCAGTTTCATCTGGTGCA+TGG	0.534293	tig0020883:+108430	None:intergenic
ACACCACGCCATATGTTCCT+CGG	0.562625	tig0020883:+109586	None:intergenic
AGATGATTAGGGAAATGATG+TGG	0.624256	tig0020883:-108109	Msa1387360:CDS
AGATTGACCGGAACTTGAGG+AGG	0.650608	tig0020883:+108158	None:intergenic
AGCAATGTATTTGATGATTG+TGG	0.514065	tig0020883:-109513	Msa1387360:CDS
AGCACTCCAACATCCATATA+TGG	0.414137	tig0020883:-108213	Msa1387360:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
TGAAGTTACTTAGACATGTT+CGG	-	tig0020883:108299-108318	Msa1387360:CDS	30.0%
TATATAAATGAGGCTTACAC+TGG	-	tig0020883:108681-108700	Msa1387360:CDS	30.0%
ATATAAATGAGGCTTACACT+GGG	-	tig0020883:108682-108701	Msa1387360:CDS	30.0%
ATTCTGCAAATATTCTTCAC+CGG	-	tig0020883:109067-109086	Msa1387360:intron	30.0%
CACTTATAATCAGTTTCATC+TGG	+	tig0020883:109343-109362	None:intergenic	30.0%
ATATCACTTCCTTATACAGA+GGG	-	tig0020883:109425-109444	Msa1387360:CDS	30.0%
ATTGGGATAACAAGATGATT+AGG	-	tig0020883:109642-109661	Msa1387360:CDS	30.0%
TTGGGATAACAAGATGATTA+GGG	-	tig0020883:109643-109662	Msa1387360:CDS	30.0%
TGAAGTTACTTAGACATGTT+CGG	-	tig0020883:108299-108318	Msa1387360:CDS	30.0%
TATATAAATGAGGCTTACAC+TGG	-	tig0020883:108681-108700	Msa1387360:CDS	30.0%

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Chromosome	Type	Strat	End	Strand	Name
tig0020883	gene	108061	109724	108061	ID=Msa1387360;Name=Msa1387360
tig0020883	mRNA	108061	109724	108061	ID=Msa1387360-mRNA-1;Parent=Msa1387360;Name=Msa1387360-mRNA-1;_AED=0.13;_eAED=0.13;_QI=0\|0\|0\|1\|1\|1\|3\|0\|334
tig0020883	exon	109302	109724	109302	ID=Msa1387360-mRNA-1:exon:10543;Parent=Msa1387360-mRNA-1
tig0020883	exon	108608	108743	108608	ID=Msa1387360-mRNA-1:exon:10542;Parent=Msa1387360-mRNA-1
tig0020883	exon	108061	108506	108061	ID=Msa1387360-mRNA-1:exon:10541;Parent=Msa1387360-mRNA-1
tig0020883	CDS	109302	109724	109302	ID=Msa1387360-mRNA-1:cds;Parent=Msa1387360-mRNA-1
tig0020883	CDS	108608	108743	108608	ID=Msa1387360-mRNA-1:cds;Parent=Msa1387360-mRNA-1
tig0020883	CDS	108061	108506	108061	ID=Msa1387360-mRNA-1:cds;Parent=Msa1387360-mRNA-1

Gene Sequence

>Msa1387360

ATGACAACTTTAGAGGAACCGACGATCAGAAAAAGGCCAAGGAAAAAGCATTGTTATACAATGTGGGAAACATTGTTTGATATTCATACAAAGTATCGTCCAATCAAGCCTATTGGCCGAGGAACATATGGCGTGGTGTGCTCATCCATCAATATGGATACCAATGAGAAAGTGGCAATCAAGAAGATTAGCAATGTATTTGATGATTGTGGTGATGCTCTGAGAACTTTAAGGGAGATGAAGTTACTTAGACATGTTCGGCATGAGAATGTGATTGCTCTGAAAGATGTTATGATACCAGACCAAAGAACAAGTTTTAAGGATATCTACTTGGTCTATGAACTTATGGATAAAGATCTTCATCGTATTATCAAATCATCTAAGCCACTTTCCAATGATCATTGCAAACTCTACTTAGTTCAGGTACTTCAAGGTCTTAATCATCTTCATTCTGCAAATATTCTTCACCGGGACCTGAAGCCTGAGAATCTGCTCGTCAACGCAAATTGTGATTTGAAGATATGTGATTTTGGGCTTGCTCGGGCAAATCAAGTTGATGGGTGCATTTTTGCTGAGATGCTTGGCAGAAAGCCAATCTTCGCTGGGAAAAATTCCATGCACCAGATGAAACTGATTATAAGTGTTCTTGGAAGCCAAGAGTCTGATCTTGAGTTAATAGTTAATCCAAAAACTAAGGCATTCATCATATCACTTCCTTATACAGAGGGGACACACTTCTCTCAGCTATACCCACAAGCAGATCCATTAGCCATAGATTTATTACAAAAAATGCTTGTGTTTGATCCAACTAAAAGAATTACTGCATCAGAAGCACTCCAACATCCATATATGGCTGATCTATGCAATGATCAATGGCGTAACCGTCCTCCTCAAGTTCCGGTCAATCTTCACATTGATGAAAATTGGGATAACAAGATGATTAGGGAAATGATGTGGAATGAGATGCTTCATTATCATCCTGAAGCTGCTTTTGGCAATGCATAG

Protein sequence

>Msa1387360

MTTLEEPTIRKRPRKKHCYTMWETLFDIHTKYRPIKPIGRGTYGVVCSSINMDTNEKVAIKKISNVFDDCGDALRTLREMKLLRHVRHENVIALKDVMIPDQRTSFKDIYLVYELMDKDLHRIIKSSKPLSNDHCKLYLVQVLQGLNHLHSANILHRDLKPENLLVNANCDLKICDFGLARANQVDGCIFAEMLGRKPIFAGKNSMHQMKLIISVLGSQESDLELIVNPKTKAFIISLPYTEGTHFSQLYPQADPLAIDLLQKMLVFDPTKRITASEALQHPYMADLCNDQWRNRPPQVPVNLHIDENWDNKMIREMMWNEMLHYHPEAAFGNA*