Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1387560 | XP_003627433.1 | 71.717 | 99 | 27 | 1 | 1 | 98 | 1 | 99 | 1.14e-37 | 135 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1387560 | sp|O80807|AGL23_ARATH | 40.789 | 76 | 45 | 0 | 11 | 86 | 1 | 76 | 4.99e-13 | 65.1 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1387560 | G7L955 | 71.717 | 99 | 27 | 1 | 1 | 98 | 1 | 99 | 5.44e-38 | 135 |
| Gene ID | Type | Classification |
|---|---|---|
| Msa1387560 | TF | MADS-M-type |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1387560 | MtrunA17_Chr8g0345001 | 71.717 | 99 | 27 | 1 | 1 | 98 | 1 | 99 | 1.05e-41 | 135 |
| Msa1387560 | MtrunA17_Chr3g0093131 | 61.538 | 52 | 19 | 1 | 35 | 85 | 37 | 88 | 8.77e-16 | 68.9 |
| Msa1387560 | MtrunA17_Chr1g0149391 | 41.333 | 75 | 44 | 0 | 13 | 87 | 5 | 79 | 2.41e-13 | 63.2 |
| Msa1387560 | MtrunA17_Chr1g0169761 | 38.462 | 78 | 48 | 0 | 18 | 95 | 3 | 80 | 2.63e-13 | 62.0 |
| Msa1387560 | MtrunA17_Chr8g0376821 | 36.709 | 79 | 50 | 0 | 18 | 96 | 3 | 81 | 2.74e-13 | 62.0 |
| Msa1387560 | MtrunA17_Chr2g0333981 | 46.575 | 73 | 37 | 2 | 13 | 84 | 8 | 79 | 2.75e-13 | 62.0 |
| Msa1387560 | MtrunA17_Chr1g0206991 | 38.462 | 78 | 48 | 0 | 13 | 90 | 5 | 82 | 5.23e-13 | 61.2 |
| Msa1387560 | MtrunA17_Chr5g0422421 | 42.857 | 70 | 40 | 0 | 18 | 87 | 10 | 79 | 9.89e-13 | 61.6 |
| Msa1387560 | MtrunA17_Chr5g0422941 | 42.857 | 70 | 40 | 0 | 18 | 87 | 10 | 79 | 1.10e-12 | 61.2 |
| Msa1387560 | MtrunA17_Chr3g0099421 | 40.000 | 75 | 45 | 0 | 13 | 87 | 30 | 104 | 8.82e-12 | 58.9 |
| Msa1387560 | MtrunA17_Chr3g0099411 | 37.333 | 75 | 47 | 0 | 13 | 87 | 5 | 79 | 9.87e-12 | 58.5 |
| Msa1387560 | MtrunA17_Chr1g0206721 | 37.333 | 75 | 47 | 0 | 13 | 87 | 5 | 79 | 1.04e-11 | 58.5 |
| Msa1387560 | MtrunA17_Chr1g0206701 | 37.333 | 75 | 47 | 0 | 13 | 87 | 5 | 79 | 1.17e-11 | 58.5 |
| Msa1387560 | MtrunA17_Chr8g0355091 | 37.333 | 75 | 47 | 0 | 13 | 87 | 5 | 79 | 1.31e-11 | 58.5 |
| Msa1387560 | MtrunA17_Chr4g0074101 | 37.662 | 77 | 48 | 0 | 18 | 94 | 3 | 79 | 1.41e-11 | 57.8 |
| Msa1387560 | MtrunA17_Chr2g0317451 | 42.647 | 68 | 39 | 0 | 18 | 85 | 10 | 77 | 1.53e-11 | 58.2 |
| Msa1387560 | MtrunA17_Chr2g0292791 | 37.333 | 75 | 47 | 0 | 13 | 87 | 5 | 79 | 1.58e-11 | 58.2 |
| Msa1387560 | MtrunA17_Chr2g0317481 | 42.647 | 68 | 39 | 0 | 18 | 85 | 10 | 77 | 1.63e-11 | 58.2 |
| Msa1387560 | MtrunA17_Chr1g0208581 | 39.474 | 76 | 46 | 0 | 10 | 85 | 2 | 77 | 2.38e-11 | 57.8 |
| Msa1387560 | MtrunA17_Chr5g0419601 | 43.284 | 67 | 38 | 0 | 18 | 84 | 10 | 76 | 2.95e-11 | 57.4 |
| Msa1387560 | MtrunA17_Chr2g0302091 | 42.667 | 75 | 33 | 3 | 15 | 87 | 35 | 101 | 3.04e-11 | 57.0 |
| Msa1387560 | MtrunA17_Chr8g0379021 | 38.462 | 78 | 48 | 0 | 7 | 84 | 2 | 79 | 3.65e-11 | 56.6 |
| Msa1387560 | MtrunA17_Chr2g0325971 | 39.706 | 68 | 41 | 0 | 18 | 85 | 10 | 77 | 4.19e-11 | 57.0 |
| Msa1387560 | MtrunA17_Chr1g0208561 | 39.706 | 68 | 41 | 0 | 18 | 85 | 10 | 77 | 7.75e-11 | 56.2 |
| Msa1387560 | MtrunA17_Chr3g0089141 | 39.706 | 68 | 41 | 0 | 18 | 85 | 10 | 77 | 8.50e-11 | 56.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1387560 | AT1G65360.1 | 40.789 | 76 | 45 | 0 | 11 | 86 | 1 | 76 | 5.08e-14 | 65.1 |
| Msa1387560 | AT3G66656.1 | 40.299 | 67 | 40 | 0 | 18 | 84 | 3 | 69 | 9.95e-13 | 60.8 |
| Msa1387560 | AT1G01530.1 | 40.260 | 77 | 46 | 0 | 11 | 87 | 1 | 77 | 2.24e-12 | 60.8 |
Find 10 sgRNAs with CRISPR-Local
Find 12 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCAGCTATCGACGCTGAAAA+AGG | 0.238437 | tig0020912:-64418 | Msa1387560:CDS |
| AAGTGTTTAATGACGATGTT+AGG | 0.253299 | tig0020912:+64457 | None:intergenic |
| CTCTGCTTTCTTGATTTCAA+TGG | 0.286601 | tig0020912:+64627 | None:intergenic |
| TAATGACGATGTTAGGGTTA+GGG | 0.414307 | tig0020912:+64464 | None:intergenic |
| TTAATGACGATGTTAGGGTT+AGG | 0.425753 | tig0020912:+64463 | None:intergenic |
| AGTGTTTAATGACGATGTTA+GGG | 0.483491 | tig0020912:+64458 | None:intergenic |
| TTTCCATCTGAAGAAGCCAT+TGG | 0.494665 | tig0020912:+64688 | None:intergenic |
| GATCCAATGGCTTCTTCAGA+TGG | 0.507351 | tig0020912:-64691 | None:intergenic |
| ACTTTCTCAAAGCTCAAACT+AGG | 0.549629 | tig0020912:-64583 | Msa1387560:CDS |
| GCATAGAATAGATAATTCTG+TGG | 0.670141 | tig0020912:+64546 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ATCGTCATTAAACACTTTTT+CGG | - | tig0020912:64645-64664 | Msa1387560:CDS | 25.0% |
| GCATAGAATAGATAATTCTG+TGG | + | tig0020912:64553-64572 | None:intergenic | 30.0% | |
| AGTGTTTAATGACGATGTTA+GGG | + | tig0020912:64641-64660 | None:intergenic | 30.0% | |
| AAGTGTTTAATGACGATGTT+AGG | + | tig0020912:64642-64661 | None:intergenic | 30.0% | |
| !!! | CATTAAACACTTTTTCGGTA+AGG | - | tig0020912:64650-64669 | Msa1387560:CDS | 30.0% |
| CTCTGCTTTCTTGATTTCAA+TGG | + | tig0020912:64472-64491 | None:intergenic | 35.0% | |
| ACTTTCTCAAAGCTCAAACT+AGG | - | tig0020912:64513-64532 | Msa1387560:CDS | 35.0% | |
| GACAAAAAACTCTATGCATG+TGG | - | tig0020912:64606-64625 | Msa1387560:CDS | 35.0% | |
| TAATGACGATGTTAGGGTTA+GGG | + | tig0020912:64635-64654 | None:intergenic | 35.0% | |
| TTAATGACGATGTTAGGGTT+AGG | + | tig0020912:64636-64655 | None:intergenic | 35.0% | |
| TCAGCTATCGACGCTGAAAA+AGG | - | tig0020912:64678-64697 | Msa1387560:CDS | 45.0% | |
| CAGCTATCGACGCTGAAAAA+GGG | - | tig0020912:64679-64698 | Msa1387560:CDS | 45.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| tig0020912 | gene | 64411 | 64707 | 64411 | ID=Msa1387560;Name=Msa1387560 |
| tig0020912 | mRNA | 64411 | 64707 | 64411 | ID=Msa1387560-mRNA-1;Parent=Msa1387560;Name=Msa1387560-mRNA-1;_AED=0.24;_eAED=0.24;_QI=0|-1|0|1|-1|1|1|0|98 |
| tig0020912 | exon | 64411 | 64707 | 64411 | ID=Msa1387560-mRNA-1:exon:10689;Parent=Msa1387560-mRNA-1 |
| tig0020912 | CDS | 64411 | 64707 | 64411 | ID=Msa1387560-mRNA-1:cds;Parent=Msa1387560-mRNA-1 |
| Gene Sequence |
| Protein sequence |
