AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1388200

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1388200 MtrunA17_Chr5g0401421 94.866 448 23 0 22 469 1 448 0.0 870
Msa1388200 MtrunA17_Chr4g0053901 66.805 482 135 10 1 469 1 470 0.0 614
Msa1388200 MtrunA17_Chr2g0323111 48.780 205 91 2 265 469 118 308 9.88e-63 206
Msa1388200 MtrunA17_Chr2g0323111 37.162 148 65 6 17 154 16 145 4.21e-16 79.3
Msa1388200 MtrunA17_Chr5g0401131 27.446 419 257 11 56 468 114 491 3.12e-44 162
Msa1388200 MtrunA17_Chr1g0159461 26.506 415 257 6 54 466 223 591 2.50e-40 152
Msa1388200 MtrunA17_Chr3g0125811 27.014 422 246 8 54 466 201 569 5.60e-40 151
Msa1388200 MtrunA17_Chr2g0329591 27.189 434 242 13 55 468 73 452 5.38e-37 141
Msa1388200 MtrunA17_Chr5g0445331 24.429 438 265 10 42 466 147 531 7.19e-35 136
Msa1388200 MtrunA17_Chr2g0319091 26.241 423 250 10 53 466 209 578 7.36e-35 137
Msa1388200 MtrunA17_Chr3g0101141 24.762 420 260 9 53 466 172 541 1.06e-34 136
Msa1388200 MtrunA17_Chr5g0447691 25.943 424 250 10 53 466 174 543 1.08e-33 133
Msa1388200 MtrunA17_Chr2g0306641 26.555 418 244 13 56 466 125 486 1.14e-33 132
Msa1388200 MtrunA17_Chr4g0076091 25.581 430 247 11 53 467 172 543 7.53e-33 130
Msa1388200 MtrunA17_Chr8g0357601 26.555 418 250 13 57 466 183 551 2.07e-32 129
Msa1388200 MtrunA17_Chr4g0031711 24.586 423 268 12 53 469 358 735 6.09e-32 129
Msa1388200 MtrunA17_Chr8g0344101 26.651 424 255 12 54 466 221 599 6.57e-31 125
Msa1388200 MtrunA17_Chr2g0290241 25.416 421 257 10 53 466 54 424 7.16e-30 120
Msa1388200 MtrunA17_Chr7g0224271 26.540 422 250 14 56 466 300 672 3.65e-29 120
Msa1388200 MtrunA17_Chr2g0328391 24.413 426 271 13 49 469 292 671 4.36e-29 120
Msa1388200 MtrunA17_Chr3g0110971 25.467 428 240 10 57 466 220 586 7.14e-29 119
Msa1388200 MtrunA17_Chr8g0376381 25.721 416 239 13 57 466 170 521 7.34e-29 119
Msa1388200 MtrunA17_Chr4g0031731 23.753 421 269 13 53 467 251 625 1.44e-28 119
Msa1388200 MtrunA17_Chr4g0031701 24.113 423 265 15 53 467 251 625 6.57e-28 117
Msa1388200 MtrunA17_Chr4g0057891 26.887 424 246 14 55 466 148 519 1.38e-27 115
Msa1388200 MtrunA17_Chr4g0031721 25.059 423 262 15 53 469 313 686 4.62e-25 108
Msa1388200 MtrunA17_Chr4g0031741 24.821 419 260 15 56 469 284 652 7.23e-25 108
Msa1388200 MtrunA17_Chr3g0085681 24.083 436 279 11 57 466 26 435 1.12e-24 105
Msa1388200 MtrunA17_Chr4g0057291 23.333 420 267 12 56 468 292 663 3.10e-24 106
Msa1388200 MtrunA17_Chr7g0236851 25.000 464 266 11 9 466 269 656 8.21e-24 104
Msa1388200 MtrunA17_Chr2g0328341 23.404 423 267 13 53 469 269 640 2.83e-23 103
Msa1388200 MtrunA17_Chr7g0238891 22.642 424 271 11 53 469 369 742 3.45e-23 103
Msa1388200 MtrunA17_Chr2g0328431 22.430 428 276 12 48 469 279 656 7.91e-22 98.6
Msa1388200 MtrunA17_Chr4g0038851 22.653 490 286 14 30 466 19 468 1.11e-21 97.4
Msa1388200 MtrunA17_Chr2g0328381 22.695 423 270 13 53 469 272 643 1.38e-21 97.8
Msa1388200 MtrunA17_Chr6g0468561 24.883 426 254 11 52 466 291 661 1.57e-21 97.8
Msa1388200 MtrunA17_Chr7g0245601 21.602 412 262 11 59 468 441 793 1.62e-21 98.2
Msa1388200 MtrunA17_Chr4g0039661 25.653 421 254 12 51 466 188 554 2.22e-21 97.1
Msa1388200 MtrunA17_Chr5g0396491 22.944 462 265 12 56 466 56 477 2.33e-21 96.7
Msa1388200 MtrunA17_Chr2g0328411 21.928 415 272 11 56 467 289 654 8.00e-21 95.9
Msa1388200 MtrunA17_Chr3g0085361 23.343 347 226 7 145 466 18 349 1.42e-20 92.8
Msa1388200 MtrunA17_Chr1g0183641 21.928 415 259 12 56 466 123 476 3.98e-19 89.7
Msa1388200 MtrunA17_Chr4g0038761 23.373 415 257 14 59 469 87 444 6.41e-19 89.0
Msa1388200 MtrunA17_Chr2g0328401 22.901 393 248 11 48 435 280 622 2.46e-18 87.8
Msa1388200 MtrunA17_Chr8g0383981 24.299 428 242 14 56 469 139 498 2.84e-17 84.3
Msa1388200 MtrunA17_Chr1g0200401 23.451 452 249 16 43 466 76 458 5.31e-16 80.1
Msa1388200 MtrunA17_Chr7g0270031 23.529 323 198 9 150 466 274 553 1.59e-14 75.9
Msa1388200 MtrunA17_Chr5g0424131 23.877 423 265 17 56 466 121 498 8.32e-14 73.6
Msa1388200 MtrunA17_Chr2g0328371 23.221 267 162 8 150 412 13 240 1.83e-13 70.1
Msa1388200 MtrunA17_Chr3g0114841 22.222 324 210 7 147 466 214 499 2.73e-13 72.0
Msa1388200 MtrunA17_Chr2g0328361 20.665 421 256 13 53 469 271 617 4.24e-13 71.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1388200 AT3G49950.1 56.979 437 148 9 35 468 10 409 2.63e-168 480
Msa1388200 AT5G67411.1 55.066 227 75 4 184 408 1 202 3.31e-75 235
Msa1388200 AT4G37650.1 28.188 447 271 12 35 469 123 531 1.80e-42 158
Msa1388200 AT2G04890.1 25.481 416 255 7 53 466 50 412 1.28e-34 134
Msa1388200 AT1G21450.1 26.005 423 250 9 54 466 223 592 4.15e-34 135
Msa1388200 AT5G48150.1 27.229 415 249 8 57 466 123 489 1.91e-33 132
Msa1388200 AT5G48150.4 27.229 415 249 8 57 466 123 489 1.91e-33 132
Msa1388200 AT5G48150.2 27.229 415 249 8 57 466 123 489 1.91e-33 132
Msa1388200 AT5G48150.3 27.229 415 249 8 57 466 123 489 1.91e-33 132
Msa1388200 AT1G50600.3 25.298 419 259 10 53 466 156 525 1.22e-32 130
Msa1388200 AT1G50600.1 25.298 419 259 10 53 466 227 596 1.88e-32 130
Msa1388200 AT1G50600.2 25.298 419 259 10 53 466 231 600 2.31e-32 130
Msa1388200 AT5G17490.1 25.604 414 249 6 55 466 159 515 4.96e-32 129
Msa1388200 AT3G03450.1 25.728 412 253 8 57 466 184 544 4.24e-31 126
Msa1388200 AT4G17230.1 24.648 426 257 9 53 468 155 526 1.76e-30 124
Msa1388200 AT4G17230.2 24.648 426 257 9 53 468 155 526 1.76e-30 124
Msa1388200 AT2G29065.1 26.014 419 265 12 56 469 252 630 2.68e-29 121
Msa1388200 AT3G13840.1 26.651 424 240 14 54 466 146 509 1.12e-27 115
Msa1388200 AT5G52510.1 23.041 434 257 11 52 466 264 639 1.79e-25 110
Msa1388200 AT1G14920.1 24.096 415 251 12 57 466 173 528 2.09e-25 109
Msa1388200 AT1G50420.1 24.342 456 266 14 56 466 57 478 6.62e-25 107
Msa1388200 AT2G01570.1 23.729 413 255 12 57 466 225 580 8.63e-25 107
Msa1388200 AT2G37650.2 23.934 422 264 14 53 468 332 702 2.46e-24 107
Msa1388200 AT2G37650.1 23.934 422 264 14 53 468 344 714 2.92e-24 106
Msa1388200 AT3G46600.2 22.931 423 270 9 53 469 1 373 2.12e-22 99.0
Msa1388200 AT1G66350.1 23.002 413 252 8 57 466 156 505 3.08e-22 99.8
Msa1388200 AT3G46600.1 23.239 426 265 9 53 469 209 581 8.98e-22 99.0
Msa1388200 AT5G59450.1 23.394 436 280 10 41 469 212 600 9.49e-22 99.0
Msa1388200 AT3G46600.3 23.005 426 266 9 53 469 177 549 1.30e-21 98.2
Msa1388200 AT1G55580.1 23.690 439 275 12 53 466 41 444 2.22e-21 96.7
Msa1388200 AT1G07520.2 24.424 434 252 17 56 469 154 531 2.48e-21 97.4
Msa1388200 AT1G07520.4 24.424 434 252 17 56 469 154 531 2.48e-21 97.4
Msa1388200 AT1G07520.1 24.424 434 252 17 56 469 318 695 3.41e-21 97.4
Msa1388200 AT1G07520.3 24.424 434 252 17 56 469 319 696 3.73e-21 97.1
Msa1388200 AT1G07530.1 20.665 421 284 10 53 469 393 767 1.83e-19 92.0
Msa1388200 AT1G63100.2 22.951 427 263 13 56 466 277 653 2.41e-19 91.7
Msa1388200 AT2G29060.1 22.642 424 272 12 53 468 318 693 2.62e-19 91.7
Msa1388200 AT2G29060.2 22.642 424 272 12 53 468 281 656 2.85e-19 91.3
Msa1388200 AT1G63100.1 22.951 427 263 13 56 466 252 628 3.50e-19 90.9
Msa1388200 AT5G66770.1 25.342 292 179 7 147 435 294 549 2.75e-17 85.1
Msa1388200 AT5G41920.1 22.782 417 256 14 56 466 43 399 3.33e-17 84.0
Msa1388200 AT3G50650.1 24.706 340 190 9 147 466 248 541 3.17e-13 72.4
Msa1388200 AT4G08250.1 24.706 425 251 19 56 466 107 476 7.37e-11 64.7

Find 77 sgRNAs with CRISPR-Local

Find 184 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GATCTTTCTTACGACGAATT+AGG 0.219981 tig0021037:+27150 Msa1388200:CDS
CGACGAATTAGGTTCAAAAT+TGG 0.230348 tig0021037:+27161 Msa1388200:CDS
AATCCGCGTTTAATTATCTT+TGG 0.234805 tig0021037:+27529 Msa1388200:CDS
GCAGCTGTTAGCTTGATTAA+AGG 0.266223 tig0021037:-27072 None:intergenic
TTTCAGTGAGGATTCGGTTT+TGG 0.295734 tig0021037:+27737 Msa1388200:CDS
TAAAACAAGAAACGTGATTA+TGG 0.317994 tig0021037:+27194 Msa1388200:CDS
AAGTGTAGTAGAGGGTTATT+AGG 0.328746 tig0021037:-26589 None:intergenic
ATGAGCTTCTTGTGCTTACT+TGG 0.341746 tig0021037:+27814 Msa1388200:CDS
AATGTAAATAGAGTTTCTTC+AGG 0.361286 tig0021037:-27351 None:intergenic
AGATTTAACATCAAGTAGTT+TGG 0.367518 tig0021037:+27494 Msa1388200:CDS
AGATCAAGCATTGGTGGAAC+TGG 0.372508 tig0021037:-27132 None:intergenic
GCATTGGTGGAACTGGAATA+TGG 0.373190 tig0021037:-27125 None:intergenic
CACTAGCACAACAAATCCTT+TGG 0.378101 tig0021037:+26680 Msa1388200:CDS
GCTTCCTGCTAGGCGTTGAT+TGG 0.395425 tig0021037:-26737 None:intergenic
TTAACACCTTGGCATCGTTT+CGG 0.442385 tig0021037:+26913 Msa1388200:CDS
TCCACCAAGACGACTATTGT+TGG 0.444785 tig0021037:-27462 None:intergenic
TCTCATTCGTTGCTCCCACT+TGG 0.447629 tig0021037:-27691 None:intergenic
ATGATAGTTATAACTATTTG+CGG 0.450034 tig0021037:-26830 None:intergenic
AGTTTGTTTCAGTGAGGATT+CGG 0.453807 tig0021037:+27731 Msa1388200:CDS
GAACAAGGACTAATAACTAC+AGG 0.460908 tig0021037:-26553 None:intergenic
TAATAAATCTCATGAAGCAT+TGG 0.465324 tig0021037:+27296 Msa1388200:CDS
CTGAGGGTGTTGTGTTGTTA+AGG 0.465694 tig0021037:-26705 None:intergenic
AAGTGTGTGGTTTGGTGAAA+AGG 0.471718 tig0021037:-26499 None:intergenic
TAGTAGAGGGTTATTAGGTG+TGG 0.478477 tig0021037:-26584 None:intergenic
GGTATGAAGCTGAAATTTGT+TGG 0.481427 tig0021037:+27607 Msa1388200:CDS
GCAATTCTAGAAGCTACTAA+AGG 0.483791 tig0021037:+26955 Msa1388200:CDS
GTCTCCAATCAACGCCTAGC+AGG 0.489729 tig0021037:+26733 Msa1388200:CDS
GAGTAACTGCTCCATGCAAT+TGG 0.490828 tig0021037:-26620 None:intergenic
ATGTTATGTTTGCTTCTGCT+TGG 0.495765 tig0021037:+27847 Msa1388200:CDS
AGAAAACTCGTGTGTTTGTA+TGG 0.500579 tig0021037:-26863 None:intergenic
CTTCTTGTGCTTACTTGGAA+AGG 0.503343 tig0021037:+27819 Msa1388200:CDS
TTCTGGAATATTGCAACTAC+AGG 0.513561 tig0021037:-26794 None:intergenic
TGATGAACATGCAGCTGGAT+GGG 0.514109 tig0021037:+27776 Msa1388200:CDS
ATTGTTGCTTTCAATTGCAC+TGG 0.516587 tig0021037:-26653 None:intergenic
GAGAATGTGATAGCATATGA+AGG 0.519545 tig0021037:+27636 Msa1388200:CDS
AGATCCAACAATAGTCGTCT+TGG 0.519549 tig0021037:+27458 Msa1388200:CDS
TCCAACAATAGTCGTCTTGG+TGG 0.526010 tig0021037:+27461 Msa1388200:CDS
AATGTGTCCACCGTGTCAAA+CGG 0.536257 tig0021037:-27555 None:intergenic
ACTAGCACAACAAATCCTTT+GGG 0.538819 tig0021037:+26681 Msa1388200:CDS
GGTTGGAAAAGTGTGTGGTT+TGG 0.543864 tig0021037:-26507 None:intergenic
CTTCAACAACGCCAATTGCA+TGG 0.549170 tig0021037:+26609 Msa1388200:CDS
AGTAGAGGGTTATTAGGTGT+GGG 0.549560 tig0021037:-26583 None:intergenic
ATGCTTGATGAACATGCAGC+TGG 0.552523 tig0021037:+27771 Msa1388200:CDS
CGTTGTTGAAGTGTAGTAGA+GGG 0.553431 tig0021037:-26597 None:intergenic
GTGGGAGCAACGAATGAGAA+AGG 0.554477 tig0021037:+27695 Msa1388200:CDS
TTGATTGGAGACGTCGTCTG+AGG 0.558311 tig0021037:-26722 None:intergenic
GATGAACATGCAGCTGGATG+GGG 0.559473 tig0021037:+27777 Msa1388200:CDS
TGAAGCTGAAATTTGTTGGA+AGG 0.560306 tig0021037:+27611 Msa1388200:CDS
GTTTCTTCAACTTATTCAGA+CGG 0.564374 tig0021037:+27228 Msa1388200:CDS
TAAGAAAGATCAAGCATTGG+TGG 0.570387 tig0021037:-27138 None:intergenic
CTCGAATGAAGCTTCCTGCT+AGG 0.577569 tig0021037:-26747 None:intergenic
TTGATGAACATGCAGCTGGA+TGG 0.579446 tig0021037:+27775 Msa1388200:CDS
TTGTGTTGTTAAGGACCCAA+AGG 0.585571 tig0021037:-26696 None:intergenic
GCAATGGCGTCGATTAGTGT+AGG 0.586251 tig0021037:-27030 None:intergenic
TGATTGGAGACGTCGTCTGA+GGG 0.588030 tig0021037:-26721 None:intergenic
TGCCTTTGTTTACTCCCTCG+TGG 0.588128 tig0021037:-27582 None:intergenic
GCGTTGTTGAAGTGTAGTAG+AGG 0.604252 tig0021037:-26598 None:intergenic
TGTGTGGTTTGGTGAAAAGG+TGG 0.606992 tig0021037:-26496 None:intergenic
GCTGTTAGCTTGATTAAAGG+AGG 0.610301 tig0021037:-27069 None:intergenic
AACCTCATGATGATTTGCAA+TGG 0.610435 tig0021037:-27046 None:intergenic
CAGCTGCAGTTAATAACTGT+AGG 0.611302 tig0021037:+27088 Msa1388200:CDS
AATGTTGCACTTGCACTCGT+AGG 0.611711 tig0021037:-27272 None:intergenic
CGCCATTGCAAATCATCATG+AGG 0.616800 tig0021037:+27044 Msa1388200:CDS
TTGGATACCGTTTGACACGG+TGG 0.625357 tig0021037:+27548 Msa1388200:CDS
TCTTTGGATACCGTTTGACA+CGG 0.625561 tig0021037:+27545 Msa1388200:CDS
AGAATGTGATAGCATATGAA+GGG 0.625664 tig0021037:+27637 Msa1388200:CDS
GAGGGAGTAAACAAAGGCAG+TGG 0.640189 tig0021037:+27586 Msa1388200:CDS
TCAAGGAGTTTGTTTCAGTG+AGG 0.641089 tig0021037:+27725 Msa1388200:CDS
GGTGTGGGAAATTCTGAACA+AGG 0.649547 tig0021037:-26568 None:intergenic
GGTAGGGTTGGAAAAGTGTG+TGG 0.650079 tig0021037:-26512 None:intergenic
TCGTAAGAAAGATCAAGCAT+TGG 0.656089 tig0021037:-27141 None:intergenic
GAGTCGAGTCTAAAACCAAG+TGG 0.658958 tig0021037:+27676 Msa1388200:CDS
AACAAGGACTAATAACTACA+GGG 0.659957 tig0021037:-26552 None:intergenic
AGTCGAGTCTAAAACCAAGT+GGG 0.662079 tig0021037:+27677 Msa1388200:CDS
TACCACGAGGGAGTAAACAA+AGG 0.668759 tig0021037:+27580 Msa1388200:CDS
GTGTAACCGAAACGATGCCA+AGG 0.704462 tig0021037:-26919 None:intergenic
TATCCAAAGATAATTAAACG+CGG 0.789557 tig0021037:-27532 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATGATAGTTATAACTATTTG+CGG - tig0021037:26833-26852 None:intergenic 20.0%
!!! ATTTTATTGATTTAACACCT+TGG + tig0021037:26902-26921 Msa1388200:CDS 20.0%
!! ATGATAGTTATAACTATTTG+CGG - tig0021037:26833-26852 None:intergenic 20.0%
!!! ATTTTATTGATTTAACACCT+TGG + tig0021037:26902-26921 Msa1388200:CDS 20.0%
!!! TTTGATTTTTGTTCATGGTA+GGG - tig0021037:26531-26550 None:intergenic 25.0%
!!! ATTTGATTTTTGTTCATGGT+AGG - tig0021037:26532-26551 None:intergenic 25.0%
! TAAAACAAGAAACGTGATTA+TGG + tig0021037:27194-27213 Msa1388200:CDS 25.0%
! TAATAAATCTCATGAAGCAT+TGG + tig0021037:27296-27315 Msa1388200:CDS 25.0%
! AATGTAAATAGAGTTTCTTC+AGG - tig0021037:27354-27373 None:intergenic 25.0%
! AGATTTAACATCAAGTAGTT+TGG + tig0021037:27494-27513 Msa1388200:CDS 25.0%
! TATCCAAAGATAATTAAACG+CGG - tig0021037:27535-27554 None:intergenic 25.0%
!!! TTTGATTTTTGTTCATGGTA+GGG - tig0021037:26531-26550 None:intergenic 25.0%
!!! ATTTGATTTTTGTTCATGGT+AGG - tig0021037:26532-26551 None:intergenic 25.0%
! TAAAACAAGAAACGTGATTA+TGG + tig0021037:27194-27213 Msa1388200:CDS 25.0%
! TAATAAATCTCATGAAGCAT+TGG + tig0021037:27296-27315 Msa1388200:CDS 25.0%
! AATGTAAATAGAGTTTCTTC+AGG - tig0021037:27354-27373 None:intergenic 25.0%
! AGATTTAACATCAAGTAGTT+TGG + tig0021037:27494-27513 Msa1388200:CDS 25.0%
! TATCCAAAGATAATTAAACG+CGG - tig0021037:27535-27554 None:intergenic 25.0%
!! AGGGATTTGATTTTTGTTCA+TGG - tig0021037:26536-26555 None:intergenic 30.0%
AACAAGGACTAATAACTACA+GGG - tig0021037:26555-26574 None:intergenic 30.0%
GTTTCTTCAACTTATTCAGA+CGG + tig0021037:27228-27247 Msa1388200:CDS 30.0%
AATCCGCGTTTAATTATCTT+TGG + tig0021037:27529-27548 Msa1388200:CDS 30.0%
AGAATGTGATAGCATATGAA+GGG + tig0021037:27637-27656 Msa1388200:CDS 30.0%
!! AGGGATTTGATTTTTGTTCA+TGG - tig0021037:26536-26555 None:intergenic 30.0%
AACAAGGACTAATAACTACA+GGG - tig0021037:26555-26574 None:intergenic 30.0%
GTTTCTTCAACTTATTCAGA+CGG + tig0021037:27228-27247 Msa1388200:CDS 30.0%
AATCCGCGTTTAATTATCTT+TGG + tig0021037:27529-27548 Msa1388200:CDS 30.0%
AGAATGTGATAGCATATGAA+GGG + tig0021037:27637-27656 Msa1388200:CDS 30.0%
! ATTTTTGTTCATGGTAGGGT+TGG - tig0021037:26527-26546 None:intergenic 35.0%
GAACAAGGACTAATAACTAC+AGG - tig0021037:26556-26575 None:intergenic 35.0%
AAGTGTAGTAGAGGGTTATT+AGG - tig0021037:26592-26611 None:intergenic 35.0%
!! ATTGTTGCTTTCAATTGCAC+TGG - tig0021037:26656-26675 None:intergenic 35.0%
ACTAGCACAACAAATCCTTT+GGG + tig0021037:26681-26700 Msa1388200:CDS 35.0%
!! TTCTGGAATATTGCAACTAC+AGG - tig0021037:26797-26816 None:intergenic 35.0%
AGAAAACTCGTGTGTTTGTA+TGG - tig0021037:26866-26885 None:intergenic 35.0%
! GCAATTCTAGAAGCTACTAA+AGG + tig0021037:26955-26974 Msa1388200:CDS 35.0%
! AACCTCATGATGATTTGCAA+TGG - tig0021037:27049-27068 None:intergenic 35.0%
!! TAAGAAAGATCAAGCATTGG+TGG - tig0021037:27141-27160 None:intergenic 35.0%
TCGTAAGAAAGATCAAGCAT+TGG - tig0021037:27144-27163 None:intergenic 35.0%
GATCTTTCTTACGACGAATT+AGG + tig0021037:27150-27169 Msa1388200:CDS 35.0%
CGACGAATTAGGTTCAAAAT+TGG + tig0021037:27161-27180 Msa1388200:CDS 35.0%
!!! CGATGTTTTTAGAAGCTGTA+CGG + tig0021037:27430-27449 Msa1388200:CDS 35.0%
GGTATGAAGCTGAAATTTGT+TGG + tig0021037:27607-27626 Msa1388200:CDS 35.0%
!! TGAAGCTGAAATTTGTTGGA+AGG + tig0021037:27611-27630 Msa1388200:CDS 35.0%
GAGAATGTGATAGCATATGA+AGG + tig0021037:27636-27655 Msa1388200:CDS 35.0%
! AGTTTGTTTCAGTGAGGATT+CGG + tig0021037:27731-27750 Msa1388200:CDS 35.0%
ATGTTATGTTTGCTTCTGCT+TGG + tig0021037:27847-27866 Msa1388200:CDS 35.0%
! ATTTTTGTTCATGGTAGGGT+TGG - tig0021037:26527-26546 None:intergenic 35.0%
GAACAAGGACTAATAACTAC+AGG - tig0021037:26556-26575 None:intergenic 35.0%
AAGTGTAGTAGAGGGTTATT+AGG - tig0021037:26592-26611 None:intergenic 35.0%
!! ATTGTTGCTTTCAATTGCAC+TGG - tig0021037:26656-26675 None:intergenic 35.0%
ACTAGCACAACAAATCCTTT+GGG + tig0021037:26681-26700 Msa1388200:CDS 35.0%
!! TTCTGGAATATTGCAACTAC+AGG - tig0021037:26797-26816 None:intergenic 35.0%
AGAAAACTCGTGTGTTTGTA+TGG - tig0021037:26866-26885 None:intergenic 35.0%
! GCAATTCTAGAAGCTACTAA+AGG + tig0021037:26955-26974 Msa1388200:CDS 35.0%
! AACCTCATGATGATTTGCAA+TGG - tig0021037:27049-27068 None:intergenic 35.0%
!! TAAGAAAGATCAAGCATTGG+TGG - tig0021037:27141-27160 None:intergenic 35.0%
TCGTAAGAAAGATCAAGCAT+TGG - tig0021037:27144-27163 None:intergenic 35.0%
GATCTTTCTTACGACGAATT+AGG + tig0021037:27150-27169 Msa1388200:CDS 35.0%
CGACGAATTAGGTTCAAAAT+TGG + tig0021037:27161-27180 Msa1388200:CDS 35.0%
!!! CGATGTTTTTAGAAGCTGTA+CGG + tig0021037:27430-27449 Msa1388200:CDS 35.0%
GGTATGAAGCTGAAATTTGT+TGG + tig0021037:27607-27626 Msa1388200:CDS 35.0%
!! TGAAGCTGAAATTTGTTGGA+AGG + tig0021037:27611-27630 Msa1388200:CDS 35.0%
GAGAATGTGATAGCATATGA+AGG + tig0021037:27636-27655 Msa1388200:CDS 35.0%
! AGTTTGTTTCAGTGAGGATT+CGG + tig0021037:27731-27750 Msa1388200:CDS 35.0%
ATGTTATGTTTGCTTCTGCT+TGG + tig0021037:27847-27866 Msa1388200:CDS 35.0%
!! AAGTGTGTGGTTTGGTGAAA+AGG - tig0021037:26502-26521 None:intergenic 40.0%
AGTAGAGGGTTATTAGGTGT+GGG - tig0021037:26586-26605 None:intergenic 40.0%
TAGTAGAGGGTTATTAGGTG+TGG - tig0021037:26587-26606 None:intergenic 40.0%
CGTTGTTGAAGTGTAGTAGA+GGG - tig0021037:26600-26619 None:intergenic 40.0%
CACTAGCACAACAAATCCTT+TGG + tig0021037:26680-26699 Msa1388200:CDS 40.0%
! TTGTGTTGTTAAGGACCCAA+AGG - tig0021037:26699-26718 None:intergenic 40.0%
TTAACACCTTGGCATCGTTT+CGG + tig0021037:26913-26932 Msa1388200:CDS 40.0%
! GCTGTTAGCTTGATTAAAGG+AGG - tig0021037:27072-27091 None:intergenic 40.0%
! GCAGCTGTTAGCTTGATTAA+AGG - tig0021037:27075-27094 None:intergenic 40.0%
CAGCTGCAGTTAATAACTGT+AGG + tig0021037:27088-27107 Msa1388200:CDS 40.0%
! TTCTAAAAACATCGAGCGCA+GGG - tig0021037:27424-27443 None:intergenic 40.0%
AGATCCAACAATAGTCGTCT+TGG + tig0021037:27458-27477 Msa1388200:CDS 40.0%
TCTTTGGATACCGTTTGACA+CGG + tig0021037:27545-27564 Msa1388200:CDS 40.0%
! TGGACACATTTTTACCACGA+GGG + tig0021037:27568-27587 Msa1388200:CDS 40.0%
! AGTCGAGTCTAAAACCAAGT+GGG + tig0021037:27677-27696 Msa1388200:CDS 40.0%
!! ATGAGAAAGGCACGTTTTCA+AGG + tig0021037:27708-27727 Msa1388200:CDS 40.0%
! TCAAGGAGTTTGTTTCAGTG+AGG + tig0021037:27725-27744 Msa1388200:CDS 40.0%
!! TTTCAGTGAGGATTCGGTTT+TGG + tig0021037:27737-27756 Msa1388200:CDS 40.0%
!!! GATTCGGTTTTGGAGGTTAA+GGG + tig0021037:27747-27766 Msa1388200:CDS 40.0%
ATGAGCTTCTTGTGCTTACT+TGG + tig0021037:27814-27833 Msa1388200:CDS 40.0%
CTTCTTGTGCTTACTTGGAA+AGG + tig0021037:27819-27838 Msa1388200:CDS 40.0%
!! AAGTGTGTGGTTTGGTGAAA+AGG - tig0021037:26502-26521 None:intergenic 40.0%
AGTAGAGGGTTATTAGGTGT+GGG - tig0021037:26586-26605 None:intergenic 40.0%
TAGTAGAGGGTTATTAGGTG+TGG - tig0021037:26587-26606 None:intergenic 40.0%
CGTTGTTGAAGTGTAGTAGA+GGG - tig0021037:26600-26619 None:intergenic 40.0%
CACTAGCACAACAAATCCTT+TGG + tig0021037:26680-26699 Msa1388200:CDS 40.0%
! TTGTGTTGTTAAGGACCCAA+AGG - tig0021037:26699-26718 None:intergenic 40.0%
TTAACACCTTGGCATCGTTT+CGG + tig0021037:26913-26932 Msa1388200:CDS 40.0%
! GCTGTTAGCTTGATTAAAGG+AGG - tig0021037:27072-27091 None:intergenic 40.0%
! GCAGCTGTTAGCTTGATTAA+AGG - tig0021037:27075-27094 None:intergenic 40.0%
CAGCTGCAGTTAATAACTGT+AGG + tig0021037:27088-27107 Msa1388200:CDS 40.0%
! TTCTAAAAACATCGAGCGCA+GGG - tig0021037:27424-27443 None:intergenic 40.0%
AGATCCAACAATAGTCGTCT+TGG + tig0021037:27458-27477 Msa1388200:CDS 40.0%
TCTTTGGATACCGTTTGACA+CGG + tig0021037:27545-27564 Msa1388200:CDS 40.0%
! TGGACACATTTTTACCACGA+GGG + tig0021037:27568-27587 Msa1388200:CDS 40.0%
! AGTCGAGTCTAAAACCAAGT+GGG + tig0021037:27677-27696 Msa1388200:CDS 40.0%
!! ATGAGAAAGGCACGTTTTCA+AGG + tig0021037:27708-27727 Msa1388200:CDS 40.0%
! TCAAGGAGTTTGTTTCAGTG+AGG + tig0021037:27725-27744 Msa1388200:CDS 40.0%
!! TTTCAGTGAGGATTCGGTTT+TGG + tig0021037:27737-27756 Msa1388200:CDS 40.0%
!!! GATTCGGTTTTGGAGGTTAA+GGG + tig0021037:27747-27766 Msa1388200:CDS 40.0%
ATGAGCTTCTTGTGCTTACT+TGG + tig0021037:27814-27833 Msa1388200:CDS 40.0%
CTTCTTGTGCTTACTTGGAA+AGG + tig0021037:27819-27838 Msa1388200:CDS 40.0%
!! TGTGTGGTTTGGTGAAAAGG+TGG - tig0021037:26499-26518 None:intergenic 45.0%
! GGTTGGAAAAGTGTGTGGTT+TGG - tig0021037:26510-26529 None:intergenic 45.0%
GGTGTGGGAAATTCTGAACA+AGG - tig0021037:26571-26590 None:intergenic 45.0%
GCGTTGTTGAAGTGTAGTAG+AGG - tig0021037:26601-26620 None:intergenic 45.0%
CTTCAACAACGCCAATTGCA+TGG + tig0021037:26609-26628 Msa1388200:CDS 45.0%
GAGTAACTGCTCCATGCAAT+TGG - tig0021037:26623-26642 None:intergenic 45.0%
!! CTGAGGGTGTTGTGTTGTTA+AGG - tig0021037:26708-26727 None:intergenic 45.0%
! GCGGTTTTTGTAACTGCTTC+TGG - tig0021037:26814-26833 None:intergenic 45.0%
CGCCATTGCAAATCATCATG+AGG + tig0021037:27044-27063 Msa1388200:CDS 45.0%
! GCATTGGTGGAACTGGAATA+TGG - tig0021037:27128-27147 None:intergenic 45.0%
!! AGATCAAGCATTGGTGGAAC+TGG - tig0021037:27135-27154 None:intergenic 45.0%
AATGTTGCACTTGCACTCGT+AGG - tig0021037:27275-27294 None:intergenic 45.0%
! CTTCTAAAAACATCGAGCGC+AGG - tig0021037:27425-27444 None:intergenic 45.0%
TCCAACAATAGTCGTCTTGG+TGG + tig0021037:27461-27480 Msa1388200:CDS 45.0%
TCCACCAAGACGACTATTGT+TGG - tig0021037:27465-27484 None:intergenic 45.0%
AATGTGTCCACCGTGTCAAA+CGG - tig0021037:27558-27577 None:intergenic 45.0%
! GTGGACACATTTTTACCACG+AGG + tig0021037:27567-27586 Msa1388200:CDS 45.0%
TACCACGAGGGAGTAAACAA+AGG + tig0021037:27580-27599 Msa1388200:CDS 45.0%
! GAGTCGAGTCTAAAACCAAG+TGG + tig0021037:27676-27695 Msa1388200:CDS 45.0%
!!! GGATTCGGTTTTGGAGGTTA+AGG + tig0021037:27746-27765 Msa1388200:CDS 45.0%
ATGCTTGATGAACATGCAGC+TGG + tig0021037:27771-27790 Msa1388200:CDS 45.0%
TTGATGAACATGCAGCTGGA+TGG + tig0021037:27775-27794 Msa1388200:CDS 45.0%
TGATGAACATGCAGCTGGAT+GGG + tig0021037:27776-27795 Msa1388200:CDS 45.0%
!! TGTGTGGTTTGGTGAAAAGG+TGG - tig0021037:26499-26518 None:intergenic 45.0%
! GGTTGGAAAAGTGTGTGGTT+TGG - tig0021037:26510-26529 None:intergenic 45.0%
GGTGTGGGAAATTCTGAACA+AGG - tig0021037:26571-26590 None:intergenic 45.0%
GCGTTGTTGAAGTGTAGTAG+AGG - tig0021037:26601-26620 None:intergenic 45.0%
CTTCAACAACGCCAATTGCA+TGG + tig0021037:26609-26628 Msa1388200:CDS 45.0%
GAGTAACTGCTCCATGCAAT+TGG - tig0021037:26623-26642 None:intergenic 45.0%
!! CTGAGGGTGTTGTGTTGTTA+AGG - tig0021037:26708-26727 None:intergenic 45.0%
! GCGGTTTTTGTAACTGCTTC+TGG - tig0021037:26814-26833 None:intergenic 45.0%
CGCCATTGCAAATCATCATG+AGG + tig0021037:27044-27063 Msa1388200:CDS 45.0%
! GCATTGGTGGAACTGGAATA+TGG - tig0021037:27128-27147 None:intergenic 45.0%
!! AGATCAAGCATTGGTGGAAC+TGG - tig0021037:27135-27154 None:intergenic 45.0%
AATGTTGCACTTGCACTCGT+AGG - tig0021037:27275-27294 None:intergenic 45.0%
! CTTCTAAAAACATCGAGCGC+AGG - tig0021037:27425-27444 None:intergenic 45.0%
TCCAACAATAGTCGTCTTGG+TGG + tig0021037:27461-27480 Msa1388200:CDS 45.0%
TCCACCAAGACGACTATTGT+TGG - tig0021037:27465-27484 None:intergenic 45.0%
AATGTGTCCACCGTGTCAAA+CGG - tig0021037:27558-27577 None:intergenic 45.0%
! GTGGACACATTTTTACCACG+AGG + tig0021037:27567-27586 Msa1388200:CDS 45.0%
TACCACGAGGGAGTAAACAA+AGG + tig0021037:27580-27599 Msa1388200:CDS 45.0%
! GAGTCGAGTCTAAAACCAAG+TGG + tig0021037:27676-27695 Msa1388200:CDS 45.0%
!!! GGATTCGGTTTTGGAGGTTA+AGG + tig0021037:27746-27765 Msa1388200:CDS 45.0%
ATGCTTGATGAACATGCAGC+TGG + tig0021037:27771-27790 Msa1388200:CDS 45.0%
TTGATGAACATGCAGCTGGA+TGG + tig0021037:27775-27794 Msa1388200:CDS 45.0%
TGATGAACATGCAGCTGGAT+GGG + tig0021037:27776-27795 Msa1388200:CDS 45.0%
! GGTAGGGTTGGAAAAGTGTG+TGG - tig0021037:26515-26534 None:intergenic 50.0%
TGATTGGAGACGTCGTCTGA+GGG - tig0021037:26724-26743 None:intergenic 50.0%
TTGATTGGAGACGTCGTCTG+AGG - tig0021037:26725-26744 None:intergenic 50.0%
CTCGAATGAAGCTTCCTGCT+AGG - tig0021037:26750-26769 None:intergenic 50.0%
GTGTAACCGAAACGATGCCA+AGG - tig0021037:26922-26941 None:intergenic 50.0%
!! GCAATGGCGTCGATTAGTGT+AGG - tig0021037:27033-27052 None:intergenic 50.0%
TTGGATACCGTTTGACACGG+TGG + tig0021037:27548-27567 Msa1388200:CDS 50.0%
! TGCCTTTGTTTACTCCCTCG+TGG - tig0021037:27585-27604 None:intergenic 50.0%
GAGGGAGTAAACAAAGGCAG+TGG + tig0021037:27586-27605 Msa1388200:CDS 50.0%
TCTCATTCGTTGCTCCCACT+TGG - tig0021037:27694-27713 None:intergenic 50.0%
GTGGGAGCAACGAATGAGAA+AGG + tig0021037:27695-27714 Msa1388200:CDS 50.0%
!! CAGTGAGGATTCGGTTTTGG+AGG + tig0021037:27740-27759 Msa1388200:CDS 50.0%
GATGAACATGCAGCTGGATG+GGG + tig0021037:27777-27796 Msa1388200:CDS 50.0%
! GGTAGGGTTGGAAAAGTGTG+TGG - tig0021037:26515-26534 None:intergenic 50.0%
TGATTGGAGACGTCGTCTGA+GGG - tig0021037:26724-26743 None:intergenic 50.0%
TTGATTGGAGACGTCGTCTG+AGG - tig0021037:26725-26744 None:intergenic 50.0%
CTCGAATGAAGCTTCCTGCT+AGG - tig0021037:26750-26769 None:intergenic 50.0%
GTGTAACCGAAACGATGCCA+AGG - tig0021037:26922-26941 None:intergenic 50.0%
!! GCAATGGCGTCGATTAGTGT+AGG - tig0021037:27033-27052 None:intergenic 50.0%
TTGGATACCGTTTGACACGG+TGG + tig0021037:27548-27567 Msa1388200:CDS 50.0%
! TGCCTTTGTTTACTCCCTCG+TGG - tig0021037:27585-27604 None:intergenic 50.0%
GAGGGAGTAAACAAAGGCAG+TGG + tig0021037:27586-27605 Msa1388200:CDS 50.0%
TCTCATTCGTTGCTCCCACT+TGG - tig0021037:27694-27713 None:intergenic 50.0%
GTGGGAGCAACGAATGAGAA+AGG + tig0021037:27695-27714 Msa1388200:CDS 50.0%
!! CAGTGAGGATTCGGTTTTGG+AGG + tig0021037:27740-27759 Msa1388200:CDS 50.0%
GATGAACATGCAGCTGGATG+GGG + tig0021037:27777-27796 Msa1388200:CDS 50.0%
GTCTCCAATCAACGCCTAGC+AGG + tig0021037:26733-26752 Msa1388200:CDS 55.0%
!! GCTTCCTGCTAGGCGTTGAT+TGG - tig0021037:26740-26759 None:intergenic 55.0%
GTCTCCAATCAACGCCTAGC+AGG + tig0021037:26733-26752 Msa1388200:CDS 55.0%
!! GCTTCCTGCTAGGCGTTGAT+TGG - tig0021037:26740-26759 None:intergenic 55.0%
Chromosome Type Strat End Strand Name
tig0021037 gene 26472 27881 26472 ID=Msa1388200;Name=Msa1388200
tig0021037 mRNA 26472 27881 26472 ID=Msa1388200-mRNA-1;Parent=Msa1388200;Name=Msa1388200-mRNA-1;_AED=0.00;_eAED=0.00;_QI=0|-1|0|1|-1|1|1|0|469
tig0021037 exon 26472 27881 26472 ID=Msa1388200-mRNA-1:exon:11008;Parent=Msa1388200-mRNA-1
tig0021037 CDS 26472 27881 26472 ID=Msa1388200-mRNA-1:cds;Parent=Msa1388200-mRNA-1
Gene Sequence

>Msa1388200

ATGACGCAATTCACATCAACATTTCCACCTTTTCACCAAACCACACACTTTTCCAACCCTACCATGAACAAAAATCAAATCCCTGTAGTTATTAGTCCTTGTTCAGAATTTCCCACACCTAATAACCCTCTACTACACTTCAACAACGCCAATTGCATGGAGCAGTTACTCATTCACTGTGCCAGTGCAATTGAAAGCAACAATGCAACACTAGCACAACAAATCCTTTGGGTCCTTAACAACACAACACCCTCAGACGACGTCTCCAATCAACGCCTAGCAGGAAGCTTCATTCGAGCTCTTACAACGCGTGCAAGAAGATCCTGTAGTTGCAATATTCCAGAAGCAGTTACAAAAACCGCAAATAGTTATAACTATCATCACAATCTCGCCATACAAACACACGAGTTTTCTGTCATTGAGTTAGCAAATTTTATTGATTTAACACCTTGGCATCGTTTCGGTTACACAGCAGCTAATTCTGCAATTCTAGAAGCTACTAAAGGATTTTCAGTTATTCACATTGTTGATTTGAGTTTAACACACTGCATGCAGATTCCTACACTAATCGACGCCATTGCAAATCATCATGAGGTTCCTCCTTTAATCAAGCTAACAGCTGCAGTTAATAACTGTAGGAATAAGAATATTAACCATATTCCAGTTCCACCAATGCTTGATCTTTCTTACGACGAATTAGGTTCAAAATTGGTTAATTTTGCTAAAACAAGAAACGTGATTATGGAATTCAAAGCTGTTTCTTCAACTTATTCAGACGGATTTGCTTCGTTGATTGAACACCTACGAGTGCAAGTGCAACATTTTAATAAATCTCATGAAGCATTGGTTATAAACTGTCACATGATGCTTCATTATATTCCTGAAGAAACTCTATTTACATTTAATGATACAAATTCAAATTTGAATTTGAATTTAAACTCTTGTTCATCCCTGCGCTCGATGTTTTTAGAAGCTGTACGGAGTTTAGATCCAACAATAGTCGTCTTGGTGGATGAAGATGCAGATTTAACATCAAGTAGTTTGGTTAGTAGACTAAAATCCGCGTTTAATTATCTTTGGATACCGTTTGACACGGTGGACACATTTTTACCACGAGGGAGTAAACAAAGGCAGTGGTATGAAGCTGAAATTTGTTGGAAGGTTGAGAATGTGATAGCATATGAAGGGATTCAGAGAGTTGAGAGAGTCGAGTCTAAAACCAAGTGGGAGCAACGAATGAGAAAGGCACGTTTTCAAGGAGTTTGTTTCAGTGAGGATTCGGTTTTGGAGGTTAAGGGTATGCTTGATGAACATGCAGCTGGATGGGGATTGAAGAAAGAAGATGAGCTTCTTGTGCTTACTTGGAAAGGACATAATGTTATGTTTGCTTCTGCTTGGTTACCTTCTTGA
Protein sequence

>Msa1388200

MTQFTSTFPPFHQTTHFSNPTMNKNQIPVVISPCSEFPTPNNPLLHFNNANCMEQLLIHCASAIESNNATLAQQILWVLNNTTPSDDVSNQRLAGSFIRALTTRARRSCSCNIPEAVTKTANSYNYHHNLAIQTHEFSVIELANFIDLTPWHRFGYTAANSAILEATKGFSVIHIVDLSLTHCMQIPTLIDAIANHHEVPPLIKLTAAVNNCRNKNINHIPVPPMLDLSYDELGSKLVNFAKTRNVIMEFKAVSSTYSDGFASLIEHLRVQVQHFNKSHEALVINCHMMLHYIPEETLFTFNDTNSNLNLNLNSCSSLRSMFLEAVRSLDPTIVVLVDEDADLTSSSLVSRLKSAFNYLWIPFDTVDTFLPRGSKQRQWYEAEICWKVENVIAYEGIQRVERVESKTKWEQRMRKARFQGVCFSEDSVLEVKGMLDEHAAGWGLKKEDELLVLTWKGHNVMFASAWLPS*