AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1391320

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1391320 MtrunA17_Chr6g0452891 74.453 137 14 2 38 174 1 116 3.89e-64 196
Msa1391320 MtrunA17_Chr6g0452881 73.203 153 34 1 30 175 561 713 1.04e-63 209
Msa1391320 MtrunA17_Chr4g0037071 80.488 82 16 0 93 174 1 82 1.63e-43 144
Msa1391320 MtrunA17_Chr1g0179281 34.848 66 43 0 98 163 829 894 2.38e-11 61.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1391320 AT4G04885.1 63.000 100 37 0 76 175 591 690 6.54e-36 132
Msa1391320 AT1G66500.1 37.654 162 68 7 37 166 124 284 6.72e-23 94.4
Msa1391320 AT5G43620.1 37.342 158 68 7 37 166 123 277 4.18e-21 89.4

Find 75 sgRNAs with CRISPR-Local

Find 249 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGACGAACCTTGAGAATATT+TGG 0.171658 tig0021418:+92401 None:intergenic
GTGACTGCTGTGCTCATCTT+TGG 0.248435 tig0021418:+92307 None:intergenic
GGTTGTTGGCTTGACGTATT+AGG 0.261888 tig0021418:+92622 None:intergenic
AAGAGTTGTTTCCGATTCTT+TGG 0.310522 tig0021418:+92710 None:intergenic
ATGCCTGTTGTTATTCCATT+AGG 0.318753 tig0021418:+91993 None:intergenic
TCTTTGACTTCCTTCTTCAT+AGG 0.322887 tig0021418:+91759 None:intergenic
ATGCATTTAGCATGAATAAT+GGG 0.333668 tig0021418:+91951 None:intergenic
ATCGCATATATAGGTGTCTT+TGG 0.354576 tig0021418:-93334 Msa1391320:CDS
TATGTAATTTGTGATTTATA+TGG 0.358933 tig0021418:-92374 Msa1391320:CDS
CCGATTCTTTGGTATGGAAT+AGG 0.359303 tig0021418:+92721 None:intergenic
TATGCATTTAGCATGAATAA+TGG 0.373205 tig0021418:+91950 None:intergenic
TATATAGGTGTCTTTGGAAC+CGG 0.377723 tig0021418:-93328 Msa1391320:CDS
GTCTTTGGAACCGGGCTATT+AGG 0.379584 tig0021418:-93319 Msa1391320:CDS
GGAATAGGAAGTGGCATAAC+AGG 0.392358 tig0021418:+92736 None:intergenic
GGCATAACAGGTCGTGCATT+TGG 0.398786 tig0021418:+92748 None:intergenic
GTATATCTTAATGCGCCTAA+TGG 0.402277 tig0021418:-92008 Msa1391320:three_prime_UTR
TAACAGGTCGTGCATTTGGC+AGG 0.413669 tig0021418:+92752 None:intergenic
TGCCCAAGGTGTTATCTCAT+TGG 0.416501 tig0021418:-92563 Msa1391320:CDS
AAATTAGAATATCCAACAAT+TGG 0.418071 tig0021418:+92601 None:intergenic
AATATCCAACAATTGGTTGT+TGG 0.433148 tig0021418:+92608 None:intergenic
TTTGCAGTAATCGCATATAT+AGG 0.434152 tig0021418:-93343 None:intergenic
TTCCGACCTTTACCTCCAGC+TGG 0.442785 tig0021418:-92688 Msa1391320:CDS
GATTCGGATGAGGTAGAATC+TGG 0.448129 tig0021418:+92650 None:intergenic
AGTGAGACGATGTGGCTAAA+AGG 0.450066 tig0021418:-92212 Msa1391320:three_prime_UTR
ATTCTACCTCATCCGAATCT+TGG 0.451748 tig0021418:-92646 Msa1391320:CDS
TTGTTTCCGATTCTTTGGTA+TGG 0.469020 tig0021418:+92715 None:intergenic
AGTAGAGCATGTGGGCCAGC+TGG 0.471650 tig0021418:+92673 None:intergenic
TCAAGCCAACAACCAATTGT+TGG 0.475162 tig0021418:-92613 Msa1391320:CDS
TCACTGTAAAACACGTCAAA+AGG 0.480580 tig0021418:+92062 None:intergenic
CTTTGGTATGGAATAGGAAG+TGG 0.486169 tig0021418:+92727 None:intergenic
GCTAAAAGGTGCAGAGACAT+TGG 0.487532 tig0021418:-92198 Msa1391320:three_prime_UTR
CCTATTCCATACCAAAGAAT+CGG 0.490580 tig0021418:-92721 Msa1391320:CDS
GACGTATTAGGACCAAGATT+CGG 0.494335 tig0021418:+92634 None:intergenic
GCGCCTAATGGAATAACAAC+AGG 0.497466 tig0021418:-91996 Msa1391320:three_prime_UTR
TGTGGGCCAGCTGGAGGTAA+AGG 0.498674 tig0021418:+92682 None:intergenic
ATTGATTTAGTTACCTTCGC+AGG 0.505170 tig0021418:+92529 None:intergenic
CATCTTTGGATTTGAATCGA+TGG 0.508546 tig0021418:+92321 None:intergenic
TTGGGAGTTAAGTCAACTCC+AGG 0.511970 tig0021418:-92179 Msa1391320:three_prime_UTR
ACAGTGATGAAACGGAGGAT+TGG 0.518817 tig0021418:-92046 Msa1391320:three_prime_UTR
CCACAAGTTGTGCATTGTCT+AGG 0.520332 tig0021418:+92344 None:intergenic
CTAAAAGGTGCAGAGACATT+GGG 0.523678 tig0021418:-92197 Msa1391320:three_prime_UTR
AGAAAATAAAGATGAAGAGT+TGG 0.524053 tig0021418:-92132 Msa1391320:three_prime_UTR
CCTAGACAATGCACAACTTG+TGG 0.528311 tig0021418:-92344 Msa1391320:CDS
AGCACAGCAGTCACATGGAT+TGG 0.529243 tig0021418:-92298 Msa1391320:CDS
AGCCAATGAGATAACACCTT+GGG 0.530219 tig0021418:+92561 None:intergenic
TAAAATACATGTGCATTATG+TGG 0.538199 tig0021418:-92089 Msa1391320:three_prime_UTR
AACAGGTCGTGCATTTGGCA+GGG 0.540179 tig0021418:+92753 None:intergenic
AAAGAAAGCGCCTAATAGCC+CGG 0.541200 tig0021418:+93309 None:intergenic
TTGATTTAGTTACCTTCGCA+GGG 0.565255 tig0021418:+92530 None:intergenic
ATGCAGGGCACCTATGAAGA+AGG 0.565948 tig0021418:-91769 Msa1391320:intron
ACAGGTCGTGCATTTGGCAG+GGG 0.566169 tig0021418:+92754 None:intergenic
ATTAGTTCACTCATTGCCCA+AGG 0.573674 tig0021418:-92577 Msa1391320:CDS
GAATATTTGGATCAAACTCA+AGG 0.580223 tig0021418:+92414 None:intergenic
ATATAGGTGTCTTTGGAACC+GGG 0.582674 tig0021418:-93327 Msa1391320:CDS
AGATGAGCACAGCAGTCACA+TGG 0.587427 tig0021418:-92303 Msa1391320:CDS
GTTTGATCCAAATATTCTCA+AGG 0.588305 tig0021418:-92408 Msa1391320:CDS
TAATGGAATAACAACAGGCA+TGG 0.604751 tig0021418:-91991 Msa1391320:three_prime_UTR
AGAGCATGTGGGCCAGCTGG+AGG 0.611285 tig0021418:+92676 None:intergenic
CAGGTCGTGCATTTGGCAGG+GGG 0.612021 tig0021418:+92755 None:intergenic
GGCTAATCAAGCCCCTGCGA+AGG 0.612023 tig0021418:-92542 Msa1391320:intron
GGCCAGCTGGAGGTAAAGGT+CGG 0.612329 tig0021418:+92686 None:intergenic
AGAATCTGGAGTAGAGCATG+TGG 0.640782 tig0021418:+92664 None:intergenic
TTAGGACCAAGATTCGGATG+AGG 0.644974 tig0021418:+92640 None:intergenic
GTTGGCAGTTCCGTTCGATG+AGG 0.647423 tig0021418:-92114 Msa1391320:three_prime_UTR
ACGGAGGATTGGATATGCAG+AGG 0.650743 tig0021418:-92035 Msa1391320:three_prime_UTR
TAGCCAATGAGATAACACCT+TGG 0.659744 tig0021418:+92560 None:intergenic
TGCATTTAGCATGAATAATG+GGG 0.666894 tig0021418:+91952 None:intergenic
TATACCGCAAAATCTTCACA+CGG 0.678850 tig0021418:+91915 None:intergenic
ATACCGCAAAATCTTCACAC+GGG 0.682049 tig0021418:+91916 None:intergenic
TGATTTAGTTACCTTCGCAG+GGG 0.687811 tig0021418:+92531 None:intergenic
GAATCTGGAGTAGAGCATGT+GGG 0.691664 tig0021418:+92665 None:intergenic
ACTCACAAACTACTTGCGAG+AGG 0.695457 tig0021418:+92232 None:intergenic
TACCGCAAAATCTTCACACG+GGG 0.732999 tig0021418:+91917 None:intergenic
AGTTTGTGAGTGAGACGATG+TGG 0.738502 tig0021418:-92220 Msa1391320:three_prime_UTR
GAAGTCAAAGAAAATGAACG+CGG 0.751197 tig0021418:-91747 Msa1391320:three_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTAAAATCATATTAACAACT+TGG + tig0021418:91862-91881 None:intergenic 15.0%
!!! ATTAATTCATTTGCAATTTT+TGG - tig0021418:92283-92302 Msa1391320:CDS 15.0%
!!! TATGTAATTTGTGATTTATA+TGG - tig0021418:92707-92726 Msa1391320:CDS 15.0%
!! AACAAAAGAATAAATATTTC+CGG + tig0021418:93281-93300 None:intergenic 15.0%
!! TTAAAATCATATTAACAACT+TGG + tig0021418:91862-91881 None:intergenic 15.0%
!!! ATTAATTCATTTGCAATTTT+TGG - tig0021418:92283-92302 Msa1391320:CDS 15.0%
!!! TATGTAATTTGTGATTTATA+TGG - tig0021418:92707-92726 Msa1391320:CDS 15.0%
!! AACAAAAGAATAAATATTTC+CGG + tig0021418:93281-93300 None:intergenic 15.0%
!! ATGTTTCTTGAACAAAAAAT+TGG + tig0021418:91799-91818 None:intergenic 20.0%
!!! AATATGATTTTAACTATGAG+TGG - tig0021418:91869-91888 Msa1391320:intron 20.0%
!!! ATATGATTTTAACTATGAGT+GGG - tig0021418:91870-91889 Msa1391320:intron 20.0%
!!! TTTTTTGAAGTAATTTCATG+TGG + tig0021418:92012-92031 None:intergenic 20.0%
!! AAATTAGAATATCCAACAAT+TGG + tig0021418:92483-92502 None:intergenic 20.0%
!! ACAGAATACAATAACAAAAT+TGG + tig0021418:92630-92649 None:intergenic 20.0%
!! ATGTTTCTTGAACAAAAAAT+TGG + tig0021418:91799-91818 None:intergenic 20.0%
!!! AATATGATTTTAACTATGAG+TGG - tig0021418:91869-91888 Msa1391320:intron 20.0%
!!! ATATGATTTTAACTATGAGT+GGG - tig0021418:91870-91889 Msa1391320:intron 20.0%
!!! TTTTTTGAAGTAATTTCATG+TGG + tig0021418:92012-92031 None:intergenic 20.0%
!! AAATTAGAATATCCAACAAT+TGG + tig0021418:92483-92502 None:intergenic 20.0%
!! ACAGAATACAATAACAAAAT+TGG + tig0021418:92630-92649 None:intergenic 20.0%
! ATCATATTAACAACTTGGAA+AGG + tig0021418:91857-91876 None:intergenic 25.0%
! TCATTTCGCAAAACATTAAA+AGG - tig0021418:91947-91966 Msa1391320:three_prime_UTR 25.0%
! CAAAACATTAAAAGGAAAGA+AGG - tig0021418:91955-91974 Msa1391320:three_prime_UTR 25.0%
! ATAACTTATCCAATCATACT+CGG + tig0021418:92233-92252 None:intergenic 25.0%
! TGTTATTGTATTCTGTTTGT+AGG - tig0021418:92634-92653 Msa1391320:CDS 25.0%
!! TTCTGTTTGTAGGATTTTAT+TGG - tig0021418:92644-92663 Msa1391320:CDS 25.0%
!!! CTCTTCATCTTTATTTTCTT+CGG + tig0021418:92948-92967 None:intergenic 25.0%
! AGAAAATAAAGATGAAGAGT+TGG - tig0021418:92949-92968 Msa1391320:intron 25.0%
! TAAAATACATGTGCATTATG+TGG - tig0021418:92992-93011 Msa1391320:intron 25.0%
! ATGCATTTAGCATGAATAAT+GGG + tig0021418:93133-93152 None:intergenic 25.0%
! TATGCATTTAGCATGAATAA+TGG + tig0021418:93134-93153 None:intergenic 25.0%
!! TTGTTTTCATCATTATATGC+AGG - tig0021418:93296-93315 Msa1391320:CDS 25.0%
!! TGTTTTCATCATTATATGCA+GGG - tig0021418:93297-93316 Msa1391320:CDS 25.0%
! ATCATATTAACAACTTGGAA+AGG + tig0021418:91857-91876 None:intergenic 25.0%
! TCATTTCGCAAAACATTAAA+AGG - tig0021418:91947-91966 Msa1391320:three_prime_UTR 25.0%
! CAAAACATTAAAAGGAAAGA+AGG - tig0021418:91955-91974 Msa1391320:three_prime_UTR 25.0%
! ATAACTTATCCAATCATACT+CGG + tig0021418:92233-92252 None:intergenic 25.0%
! TGTTATTGTATTCTGTTTGT+AGG - tig0021418:92634-92653 Msa1391320:CDS 25.0%
!! TTCTGTTTGTAGGATTTTAT+TGG - tig0021418:92644-92663 Msa1391320:CDS 25.0%
!!! CTCTTCATCTTTATTTTCTT+CGG + tig0021418:92948-92967 None:intergenic 25.0%
! AGAAAATAAAGATGAAGAGT+TGG - tig0021418:92949-92968 Msa1391320:intron 25.0%
! TAAAATACATGTGCATTATG+TGG - tig0021418:92992-93011 Msa1391320:intron 25.0%
! ATGCATTTAGCATGAATAAT+GGG + tig0021418:93133-93152 None:intergenic 25.0%
! TATGCATTTAGCATGAATAA+TGG + tig0021418:93134-93153 None:intergenic 25.0%
!! TTGTTTTCATCATTATATGC+AGG - tig0021418:93296-93315 Msa1391320:CDS 25.0%
!! TGTTTTCATCATTATATGCA+GGG - tig0021418:93297-93316 Msa1391320:CDS 25.0%
!! TTGTTGATGATGCCAATTAA+TGG - tig0021418:91898-91917 Msa1391320:intron 30.0%
!! TGTTGATGATGCCAATTAAT+GGG - tig0021418:91899-91918 Msa1391320:intron 30.0%
AAATGAAGACTTAGGATACT+TGG + tig0021418:91933-91952 None:intergenic 30.0%
ATCTATTCTGATGTTTCATG+TGG + tig0021418:92080-92099 None:intergenic 30.0%
TATAATCCATGGGAAAATTC+AGG + tig0021418:92127-92146 None:intergenic 30.0%
! TGATTCGGATTATAATCCAT+GGG + tig0021418:92137-92156 None:intergenic 30.0%
AAGTGAAGATGAAACTGATT+CGG + tig0021418:92152-92171 None:intergenic 30.0%
AATATCCAACAATTGGTTGT+TGG + tig0021418:92476-92495 None:intergenic 30.0%
GAATATTTGGATCAAACTCA+AGG + tig0021418:92670-92689 None:intergenic 30.0%
GTTTGATCCAAATATTCTCA+AGG - tig0021418:92673-92692 Msa1391320:CDS 30.0%
!! GCTTACAGTTTTTAGACATA+CGG + tig0021418:92823-92842 None:intergenic 30.0%
!!! TTCATCTTTATTTTCTTCGG+TGG + tig0021418:92945-92964 None:intergenic 30.0%
!!! GTATTTTAATCCTCATCGAA+CGG + tig0021418:92980-92999 None:intergenic 30.0%
TGCATTTAGCATGAATAATG+GGG + tig0021418:93132-93151 None:intergenic 30.0%
! AAGATTTTGCGGTATATGAA+GGG - tig0021418:93173-93192 Msa1391320:intron 30.0%
CATGATGTTATTATTGCAAG+TGG - tig0021418:93229-93248 Msa1391320:intron 30.0%
!! TTGTTGATGATGCCAATTAA+TGG - tig0021418:91898-91917 Msa1391320:intron 30.0%
!! TGTTGATGATGCCAATTAAT+GGG - tig0021418:91899-91918 Msa1391320:intron 30.0%
AAATGAAGACTTAGGATACT+TGG + tig0021418:91933-91952 None:intergenic 30.0%
ATCTATTCTGATGTTTCATG+TGG + tig0021418:92080-92099 None:intergenic 30.0%
TATAATCCATGGGAAAATTC+AGG + tig0021418:92127-92146 None:intergenic 30.0%
! TGATTCGGATTATAATCCAT+GGG + tig0021418:92137-92156 None:intergenic 30.0%
AAGTGAAGATGAAACTGATT+CGG + tig0021418:92152-92171 None:intergenic 30.0%
AATATCCAACAATTGGTTGT+TGG + tig0021418:92476-92495 None:intergenic 30.0%
GAATATTTGGATCAAACTCA+AGG + tig0021418:92670-92689 None:intergenic 30.0%
GTTTGATCCAAATATTCTCA+AGG - tig0021418:92673-92692 Msa1391320:CDS 30.0%
!! GCTTACAGTTTTTAGACATA+CGG + tig0021418:92823-92842 None:intergenic 30.0%
!!! TTCATCTTTATTTTCTTCGG+TGG + tig0021418:92945-92964 None:intergenic 30.0%
!!! GTATTTTAATCCTCATCGAA+CGG + tig0021418:92980-92999 None:intergenic 30.0%
TGCATTTAGCATGAATAATG+GGG + tig0021418:93132-93151 None:intergenic 30.0%
! AAGATTTTGCGGTATATGAA+GGG - tig0021418:93173-93192 Msa1391320:intron 30.0%
CATGATGTTATTATTGCAAG+TGG - tig0021418:93229-93248 Msa1391320:intron 30.0%
ATCGCATATATAGGTGTCTT+TGG - tig0021418:91747-91766 Msa1391320:three_prime_UTR 35.0%
!! TATATAGGTGTCTTTGGAAC+CGG - tig0021418:91753-91772 Msa1391320:three_prime_UTR 35.0%
CTTCAGTCACATAACTTACT+TGG + tig0021418:91827-91846 None:intergenic 35.0%
!! GTTGATGATGCCAATTAATG+GGG - tig0021418:91900-91919 Msa1391320:intron 35.0%
TGGAAATGAACCCCATTAAT+TGG + tig0021418:91913-91932 None:intergenic 35.0%
! GTTTTGCGAAATGAAGACTT+AGG + tig0021418:91941-91960 None:intergenic 35.0%
AAGGAAAGAAGGAAATCTAG+AGG - tig0021418:91966-91985 Msa1391320:three_prime_UTR 35.0%
! TGATTACCTGAATTTTCCCA+TGG - tig0021418:92118-92137 Msa1391320:three_prime_UTR 35.0%
!! CTGATTCGGATTATAATCCA+TGG + tig0021418:92138-92157 None:intergenic 35.0%
TATTTGACAGTCGAGATTAG+AGG - tig0021418:92202-92221 Msa1391320:three_prime_UTR 35.0%
TGGTGATCTTTGCAATAGTT+TGG - tig0021418:92303-92322 Msa1391320:CDS 35.0%
CCTATTCCATACCAAAGAAT+CGG - tig0021418:92360-92379 Msa1391320:CDS 35.0%
TTGTTTCCGATTCTTTGGTA+TGG + tig0021418:92369-92388 None:intergenic 35.0%
! AAGAGTTGTTTCCGATTCTT+TGG + tig0021418:92374-92393 None:intergenic 35.0%
TTGATTTAGTTACCTTCGCA+GGG + tig0021418:92554-92573 None:intergenic 35.0%
! ATTGATTTAGTTACCTTCGC+AGG + tig0021418:92555-92574 None:intergenic 35.0%
ACGCAATCAAGTAAAATGGA+AGG + tig0021418:92606-92625 None:intergenic 35.0%
TGGTACGCAATCAAGTAAAA+TGG + tig0021418:92610-92629 None:intergenic 35.0%
TGACGAACCTTGAGAATATT+TGG + tig0021418:92683-92702 None:intergenic 35.0%
CATCTTTGGATTTGAATCGA+TGG + tig0021418:92763-92782 None:intergenic 35.0%
!! TTTATTTTCTTCGGTGGTCT+CGG + tig0021418:92939-92958 None:intergenic 35.0%
TCACTGTAAAACACGTCAAA+AGG + tig0021418:93022-93041 None:intergenic 35.0%
! CGTGTTTTACAGTGATGAAA+CGG - tig0021418:93027-93046 Msa1391320:intron 35.0%
GTATATCTTAATGCGCCTAA+TGG - tig0021418:93073-93092 Msa1391320:intron 35.0%
! ATGCCTGTTGTTATTCCATT+AGG + tig0021418:93091-93110 None:intergenic 35.0%
TAATGGAATAACAACAGGCA+TGG - tig0021418:93090-93109 Msa1391320:intron 35.0%
TATACCGCAAAATCTTCACA+CGG + tig0021418:93169-93188 None:intergenic 35.0%
! GAAGATTTTGCGGTATATGA+AGG - tig0021418:93172-93191 Msa1391320:intron 35.0%
! AGATTTTGCGGTATATGAAG+GGG - tig0021418:93174-93193 Msa1391320:intron 35.0%
! TCTTTGACTTCCTTCTTCAT+AGG + tig0021418:93325-93344 None:intergenic 35.0%
GAAGTCAAAGAAAATGAACG+CGG - tig0021418:93334-93353 Msa1391320:CDS 35.0%
ATCGCATATATAGGTGTCTT+TGG - tig0021418:91747-91766 Msa1391320:three_prime_UTR 35.0%
!! TATATAGGTGTCTTTGGAAC+CGG - tig0021418:91753-91772 Msa1391320:three_prime_UTR 35.0%
CTTCAGTCACATAACTTACT+TGG + tig0021418:91827-91846 None:intergenic 35.0%
!! GTTGATGATGCCAATTAATG+GGG - tig0021418:91900-91919 Msa1391320:intron 35.0%
TGGAAATGAACCCCATTAAT+TGG + tig0021418:91913-91932 None:intergenic 35.0%
! GTTTTGCGAAATGAAGACTT+AGG + tig0021418:91941-91960 None:intergenic 35.0%
AAGGAAAGAAGGAAATCTAG+AGG - tig0021418:91966-91985 Msa1391320:three_prime_UTR 35.0%
! TGATTACCTGAATTTTCCCA+TGG - tig0021418:92118-92137 Msa1391320:three_prime_UTR 35.0%
!! CTGATTCGGATTATAATCCA+TGG + tig0021418:92138-92157 None:intergenic 35.0%
TATTTGACAGTCGAGATTAG+AGG - tig0021418:92202-92221 Msa1391320:three_prime_UTR 35.0%
TGGTGATCTTTGCAATAGTT+TGG - tig0021418:92303-92322 Msa1391320:CDS 35.0%
CCTATTCCATACCAAAGAAT+CGG - tig0021418:92360-92379 Msa1391320:CDS 35.0%
TTGTTTCCGATTCTTTGGTA+TGG + tig0021418:92369-92388 None:intergenic 35.0%
! AAGAGTTGTTTCCGATTCTT+TGG + tig0021418:92374-92393 None:intergenic 35.0%
TTGATTTAGTTACCTTCGCA+GGG + tig0021418:92554-92573 None:intergenic 35.0%
! ATTGATTTAGTTACCTTCGC+AGG + tig0021418:92555-92574 None:intergenic 35.0%
ACGCAATCAAGTAAAATGGA+AGG + tig0021418:92606-92625 None:intergenic 35.0%
TGGTACGCAATCAAGTAAAA+TGG + tig0021418:92610-92629 None:intergenic 35.0%
TGACGAACCTTGAGAATATT+TGG + tig0021418:92683-92702 None:intergenic 35.0%
CATCTTTGGATTTGAATCGA+TGG + tig0021418:92763-92782 None:intergenic 35.0%
!! TTTATTTTCTTCGGTGGTCT+CGG + tig0021418:92939-92958 None:intergenic 35.0%
TCACTGTAAAACACGTCAAA+AGG + tig0021418:93022-93041 None:intergenic 35.0%
! CGTGTTTTACAGTGATGAAA+CGG - tig0021418:93027-93046 Msa1391320:intron 35.0%
GTATATCTTAATGCGCCTAA+TGG - tig0021418:93073-93092 Msa1391320:intron 35.0%
! ATGCCTGTTGTTATTCCATT+AGG + tig0021418:93091-93110 None:intergenic 35.0%
TAATGGAATAACAACAGGCA+TGG - tig0021418:93090-93109 Msa1391320:intron 35.0%
TATACCGCAAAATCTTCACA+CGG + tig0021418:93169-93188 None:intergenic 35.0%
! GAAGATTTTGCGGTATATGA+AGG - tig0021418:93172-93191 Msa1391320:intron 35.0%
! AGATTTTGCGGTATATGAAG+GGG - tig0021418:93174-93193 Msa1391320:intron 35.0%
! TCTTTGACTTCCTTCTTCAT+AGG + tig0021418:93325-93344 None:intergenic 35.0%
!! ATATAGGTGTCTTTGGAACC+GGG - tig0021418:91754-91773 Msa1391320:three_prime_UTR 40.0%
CTTTGGTATGGAATAGGAAG+TGG + tig0021418:92357-92376 None:intergenic 40.0%
CCGATTCTTTGGTATGGAAT+AGG + tig0021418:92363-92382 None:intergenic 40.0%
ATTCTACCTCATCCGAATCT+TGG - tig0021418:92435-92454 Msa1391320:intron 40.0%
GACGTATTAGGACCAAGATT+CGG + tig0021418:92450-92469 None:intergenic 40.0%
TCAAGCCAACAACCAATTGT+TGG - tig0021418:92468-92487 Msa1391320:intron 40.0%
ATTAGTTCACTCATTGCCCA+AGG - tig0021418:92504-92523 Msa1391320:intron 40.0%
AGCCAATGAGATAACACCTT+GGG + tig0021418:92523-92542 None:intergenic 40.0%
TAGCCAATGAGATAACACCT+TGG + tig0021418:92524-92543 None:intergenic 40.0%
TGATTTAGTTACCTTCGCAG+GGG + tig0021418:92553-92572 None:intergenic 40.0%
! CTAAAAGGTGCAGAGACATT+GGG - tig0021418:92884-92903 Msa1391320:intron 40.0%
! GTTTTACAGTGATGAAACGG+AGG - tig0021418:93030-93049 Msa1391320:intron 40.0%
ATACCGCAAAATCTTCACAC+GGG + tig0021418:93168-93187 None:intergenic 40.0%
!! AAGTGGACTTTTGCATATGC+TGG - tig0021418:93246-93265 Msa1391320:intron 40.0%
!! ATATAGGTGTCTTTGGAACC+GGG - tig0021418:91754-91773 Msa1391320:three_prime_UTR 40.0%
CTTTGGTATGGAATAGGAAG+TGG + tig0021418:92357-92376 None:intergenic 40.0%
CCGATTCTTTGGTATGGAAT+AGG + tig0021418:92363-92382 None:intergenic 40.0%
ATTCTACCTCATCCGAATCT+TGG - tig0021418:92435-92454 Msa1391320:intron 40.0%
GACGTATTAGGACCAAGATT+CGG + tig0021418:92450-92469 None:intergenic 40.0%
TCAAGCCAACAACCAATTGT+TGG - tig0021418:92468-92487 Msa1391320:intron 40.0%
ATTAGTTCACTCATTGCCCA+AGG - tig0021418:92504-92523 Msa1391320:intron 40.0%
AGCCAATGAGATAACACCTT+GGG + tig0021418:92523-92542 None:intergenic 40.0%
TAGCCAATGAGATAACACCT+TGG + tig0021418:92524-92543 None:intergenic 40.0%
TGATTTAGTTACCTTCGCAG+GGG + tig0021418:92553-92572 None:intergenic 40.0%
! CTAAAAGGTGCAGAGACATT+GGG - tig0021418:92884-92903 Msa1391320:intron 40.0%
! GTTTTACAGTGATGAAACGG+AGG - tig0021418:93030-93049 Msa1391320:intron 40.0%
ATACCGCAAAATCTTCACAC+GGG + tig0021418:93168-93187 None:intergenic 40.0%
!! AAGTGGACTTTTGCATATGC+TGG - tig0021418:93246-93265 Msa1391320:intron 40.0%
!! AAAGAAAGCGCCTAATAGCC+CGG + tig0021418:91775-91794 None:intergenic 45.0%
!! GAGGAGTATCCGAGTATGAT+TGG - tig0021418:92221-92240 Msa1391320:three_prime_UTR 45.0%
GGAATAGGAAGTGGCATAAC+AGG + tig0021418:92348-92367 None:intergenic 45.0%
! GAATCTGGAGTAGAGCATGT+GGG + tig0021418:92419-92438 None:intergenic 45.0%
! AGAATCTGGAGTAGAGCATG+TGG + tig0021418:92420-92439 None:intergenic 45.0%
GATTCGGATGAGGTAGAATC+TGG + tig0021418:92434-92453 None:intergenic 45.0%
TTAGGACCAAGATTCGGATG+AGG + tig0021418:92444-92463 None:intergenic 45.0%
!! GGTTGTTGGCTTGACGTATT+AGG + tig0021418:92462-92481 None:intergenic 45.0%
! TGCCCAAGGTGTTATCTCAT+TGG - tig0021418:92518-92537 Msa1391320:intron 45.0%
CCACAAGTTGTGCATTGTCT+AGG + tig0021418:92740-92759 None:intergenic 45.0%
CCTAGACAATGCACAACTTG+TGG - tig0021418:92737-92756 Msa1391320:CDS 45.0%
ACTCACAAACTACTTGCGAG+AGG + tig0021418:92852-92871 None:intergenic 45.0%
AGTTTGTGAGTGAGACGATG+TGG - tig0021418:92861-92880 Msa1391320:intron 45.0%
AGTGAGACGATGTGGCTAAA+AGG - tig0021418:92869-92888 Msa1391320:intron 45.0%
! GCTAAAAGGTGCAGAGACAT+TGG - tig0021418:92883-92902 Msa1391320:intron 45.0%
TTGGGAGTTAAGTCAACTCC+AGG - tig0021418:92902-92921 Msa1391320:intron 45.0%
ACAGTGATGAAACGGAGGAT+TGG - tig0021418:93035-93054 Msa1391320:intron 45.0%
GCGCCTAATGGAATAACAAC+AGG - tig0021418:93085-93104 Msa1391320:intron 45.0%
! TACCCCGTGTGAAGATTTTG+CGG - tig0021418:93162-93181 Msa1391320:intron 45.0%
TACCGCAAAATCTTCACACG+GGG + tig0021418:93167-93186 None:intergenic 45.0%
CATATGCTGGTGTTTCAAGC+CGG - tig0021418:93259-93278 Msa1391320:intron 45.0%
!! AAAGAAAGCGCCTAATAGCC+CGG + tig0021418:91775-91794 None:intergenic 45.0%
!! GAGGAGTATCCGAGTATGAT+TGG - tig0021418:92221-92240 Msa1391320:three_prime_UTR 45.0%
GGAATAGGAAGTGGCATAAC+AGG + tig0021418:92348-92367 None:intergenic 45.0%
! GAATCTGGAGTAGAGCATGT+GGG + tig0021418:92419-92438 None:intergenic 45.0%
! AGAATCTGGAGTAGAGCATG+TGG + tig0021418:92420-92439 None:intergenic 45.0%
GATTCGGATGAGGTAGAATC+TGG + tig0021418:92434-92453 None:intergenic 45.0%
TTAGGACCAAGATTCGGATG+AGG + tig0021418:92444-92463 None:intergenic 45.0%
!! GGTTGTTGGCTTGACGTATT+AGG + tig0021418:92462-92481 None:intergenic 45.0%
! TGCCCAAGGTGTTATCTCAT+TGG - tig0021418:92518-92537 Msa1391320:intron 45.0%
CCACAAGTTGTGCATTGTCT+AGG + tig0021418:92740-92759 None:intergenic 45.0%
CCTAGACAATGCACAACTTG+TGG - tig0021418:92737-92756 Msa1391320:CDS 45.0%
ACTCACAAACTACTTGCGAG+AGG + tig0021418:92852-92871 None:intergenic 45.0%
AGTTTGTGAGTGAGACGATG+TGG - tig0021418:92861-92880 Msa1391320:intron 45.0%
AGTGAGACGATGTGGCTAAA+AGG - tig0021418:92869-92888 Msa1391320:intron 45.0%
! GCTAAAAGGTGCAGAGACAT+TGG - tig0021418:92883-92902 Msa1391320:intron 45.0%
TTGGGAGTTAAGTCAACTCC+AGG - tig0021418:92902-92921 Msa1391320:intron 45.0%
ACAGTGATGAAACGGAGGAT+TGG - tig0021418:93035-93054 Msa1391320:intron 45.0%
GCGCCTAATGGAATAACAAC+AGG - tig0021418:93085-93104 Msa1391320:intron 45.0%
! TACCCCGTGTGAAGATTTTG+CGG - tig0021418:93162-93181 Msa1391320:intron 45.0%
TACCGCAAAATCTTCACACG+GGG + tig0021418:93167-93186 None:intergenic 45.0%
CATATGCTGGTGTTTCAAGC+CGG - tig0021418:93259-93278 Msa1391320:intron 45.0%
!! GTCTTTGGAACCGGGCTATT+AGG - tig0021418:91762-91781 Msa1391320:three_prime_UTR 50.0%
AACAGGTCGTGCATTTGGCA+GGG + tig0021418:92331-92350 None:intergenic 50.0%
TAACAGGTCGTGCATTTGGC+AGG + tig0021418:92332-92351 None:intergenic 50.0%
GGCATAACAGGTCGTGCATT+TGG + tig0021418:92336-92355 None:intergenic 50.0%
GTGACTGCTGTGCTCATCTT+TGG + tig0021418:92777-92796 None:intergenic 50.0%
AGATGAGCACAGCAGTCACA+TGG - tig0021418:92778-92797 Msa1391320:intron 50.0%
AGCACAGCAGTCACATGGAT+TGG - tig0021418:92783-92802 Msa1391320:intron 50.0%
GTCTCGGTTGGCAAAAAACC+TGG + tig0021418:92923-92942 None:intergenic 50.0%
! TTTTCTTCGGTGGTCTCGGT+TGG + tig0021418:92935-92954 None:intergenic 50.0%
ACGGAGGATTGGATATGCAG+AGG - tig0021418:93046-93065 Msa1391320:intron 50.0%
! ATGCAGGGCACCTATGAAGA+AGG - tig0021418:93312-93331 Msa1391320:CDS 50.0%
!! GTCTTTGGAACCGGGCTATT+AGG - tig0021418:91762-91781 Msa1391320:three_prime_UTR 50.0%
AACAGGTCGTGCATTTGGCA+GGG + tig0021418:92331-92350 None:intergenic 50.0%
TAACAGGTCGTGCATTTGGC+AGG + tig0021418:92332-92351 None:intergenic 50.0%
GGCATAACAGGTCGTGCATT+TGG + tig0021418:92336-92355 None:intergenic 50.0%
GTGACTGCTGTGCTCATCTT+TGG + tig0021418:92777-92796 None:intergenic 50.0%
AGATGAGCACAGCAGTCACA+TGG - tig0021418:92778-92797 Msa1391320:intron 50.0%
AGCACAGCAGTCACATGGAT+TGG - tig0021418:92783-92802 Msa1391320:intron 50.0%
GTCTCGGTTGGCAAAAAACC+TGG + tig0021418:92923-92942 None:intergenic 50.0%
! TTTTCTTCGGTGGTCTCGGT+TGG + tig0021418:92935-92954 None:intergenic 50.0%
ACGGAGGATTGGATATGCAG+AGG - tig0021418:93046-93065 Msa1391320:intron 50.0%
! ATGCAGGGCACCTATGAAGA+AGG - tig0021418:93312-93331 Msa1391320:CDS 50.0%
ACAGGTCGTGCATTTGGCAG+GGG + tig0021418:92330-92349 None:intergenic 55.0%
TTCCGACCTTTACCTCCAGC+TGG - tig0021418:92393-92412 Msa1391320:CDS 55.0%
GTTGGCAGTTCCGTTCGATG+AGG - tig0021418:92967-92986 Msa1391320:intron 55.0%
ACAGGTCGTGCATTTGGCAG+GGG + tig0021418:92330-92349 None:intergenic 55.0%
TTCCGACCTTTACCTCCAGC+TGG - tig0021418:92393-92412 Msa1391320:CDS 55.0%
GTTGGCAGTTCCGTTCGATG+AGG - tig0021418:92967-92986 Msa1391320:intron 55.0%
CAGGTCGTGCATTTGGCAGG+GGG + tig0021418:92329-92348 None:intergenic 60.0%
GGCCAGCTGGAGGTAAAGGT+CGG + tig0021418:92398-92417 None:intergenic 60.0%
TGTGGGCCAGCTGGAGGTAA+AGG + tig0021418:92402-92421 None:intergenic 60.0%
AGTAGAGCATGTGGGCCAGC+TGG + tig0021418:92411-92430 None:intergenic 60.0%
GGCTAATCAAGCCCCTGCGA+AGG - tig0021418:92539-92558 Msa1391320:intron 60.0%
CAGGTCGTGCATTTGGCAGG+GGG + tig0021418:92329-92348 None:intergenic 60.0%
GGCCAGCTGGAGGTAAAGGT+CGG + tig0021418:92398-92417 None:intergenic 60.0%
TGTGGGCCAGCTGGAGGTAA+AGG + tig0021418:92402-92421 None:intergenic 60.0%
AGTAGAGCATGTGGGCCAGC+TGG + tig0021418:92411-92430 None:intergenic 60.0%
GGCTAATCAAGCCCCTGCGA+AGG - tig0021418:92539-92558 Msa1391320:intron 60.0%
AGAGCATGTGGGCCAGCTGG+AGG + tig0021418:92408-92427 None:intergenic 65.0%
AGAGCATGTGGGCCAGCTGG+AGG + tig0021418:92408-92427 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
tig0021418 gene 91747 93356 91747 ID=Msa1391320;Name=Msa1391320
tig0021418 mRNA 91747 93356 91747 ID=Msa1391320-mRNA-1;Parent=Msa1391320;Name=Msa1391320-mRNA-1;_AED=0.12;_eAED=0.12;_QI=0|0.66|0.5|0.75|0.66|0.25|4|372|175
tig0021418 exon 91747 91785 91747 ID=Msa1391320-mRNA-1:exon:12616;Parent=Msa1391320-mRNA-1
tig0021418 exon 91908 92447 91908 ID=Msa1391320-mRNA-1:exon:12615;Parent=Msa1391320-mRNA-1
tig0021418 exon 92543 92782 92543 ID=Msa1391320-mRNA-1:exon:12614;Parent=Msa1391320-mRNA-1
tig0021418 exon 93276 93356 93276 ID=Msa1391320-mRNA-1:exon:12613;Parent=Msa1391320-mRNA-1
tig0021418 CDS 93276 93356 93276 ID=Msa1391320-mRNA-1:cds;Parent=Msa1391320-mRNA-1
tig0021418 CDS 92543 92782 92543 ID=Msa1391320-mRNA-1:cds;Parent=Msa1391320-mRNA-1
tig0021418 CDS 92241 92447 92241 ID=Msa1391320-mRNA-1:cds;Parent=Msa1391320-mRNA-1
tig0021418 three_prime_UTR 91908 92240 91908 ID=Msa1391320-mRNA-1:three_prime_utr;Parent=Msa1391320-mRNA-1
tig0021418 three_prime_UTR 91747 91785 91747 ID=Msa1391320-mRNA-1:three_prime_utr;Parent=Msa1391320-mRNA-1
Gene Sequence

>Msa1391320

ATCGCATATATAGGTGTCTTTGGAACCGGGCTATTAGGCGCTTTCTTTTCCAATTTTTTGTTCAAGAAACATCATTTCCAATTTGGCCCCCTGCCAAATGCACGACCTGTTATGCCACTTCCTATTCCATACCAAAGAATCGGAAACAACTCTTTCCGACCTTTACCTCCAGCTGGCCCACATGCTCTACTCCAGATTCTACCTCATCCGAATCTTGGTCCTAATACGTCAAGCCAACAACCAATTGTTGGATATTCTAATTTGATTAGTTCACTCATTGCCCAAGGTGTTATCTCATTGGCTAATCAAGCCCCTGCGAAGGATTTTATTGGCCTTGAGTTTGATCCAAATATTCTCAAGGTTCGTCATGAATATGTAATTTGTGATTTATATGGTGATTTTCCTAGACAATGCACAACTTGTGGCCATCGATTCAAATCCAAAGATGAGCACAGCAGTCACATGGATTGGCATGTGACAAAGAACCGTATGTCTAAAAACTGTAAGCAAAAGTCCTCTCGCAAGTAG
Protein sequence

>Msa1391320

IAYIGVFGTGLLGAFFSNFLFKKHHFQFGPLPNARPVMPLPIPYQRIGNNSFRPLPPAGPHALLQILPHPNLGPNTSSQQPIVGYSNLISSLIAQGVISLANQAPAKDFIGLEFDPNILKVRHEYVICDLYGDFPRQCTTCGHRFKSKDEHSSHMDWHVTKNRMSKNCKQKSSRK*