Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1395000 | AET04474.1 | 88.816 | 152 | 14 | 1 | 4 | 152 | 5 | 156 | 1.22e-85 | 258 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1395000 | G7L9K0 | 88.816 | 152 | 14 | 1 | 4 | 152 | 5 | 156 | 5.84e-86 | 258 |
| Gene ID | Type | Classification |
|---|---|---|
| Msa1395000 | TF | C2C2-GATA |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 31 sgRNAs with CRISPR-Local
Find 38 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CATAAAACTGGCTTCTCTTC+TGG | 0.255261 | tig0021904:-6054 | None:intergenic |
| TCGAACAATAAGGCCTCTTC+TGG | 0.271838 | tig0021904:-6503 | None:intergenic |
| TTCATACCTTCAAATGAAAT+TGG | 0.274802 | tig0021904:+6539 | Msa1395000:CDS |
| CTTCAAGATTTCCCCAGTTT+TGG | 0.287698 | tig0021904:-6098 | None:intergenic |
| TGGCGCCATGCTGGAGTTGC+TGG | 0.300530 | tig0021904:-6027 | None:intergenic |
| GATGTTTCTGATTCAGATAA+TGG | 0.318368 | tig0021904:+6213 | Msa1395000:CDS |
| ATCCTCAAACTACGCCTCTT+TGG | 0.326587 | tig0021904:-6401 | None:intergenic |
| CTGTTCGTAACAAGTTCTTT+TGG | 0.340372 | tig0021904:-6144 | None:intergenic |
| TCTGGTCCCTGGCGCCATGC+TGG | 0.378150 | tig0021904:-6036 | None:intergenic |
| CCAAGACCAATTTCATTTGA+AGG | 0.378872 | tig0021904:-6545 | None:intergenic |
| CCTTCAAATGAAATTGGTCT+TGG | 0.383519 | tig0021904:+6545 | Msa1395000:CDS |
| GTGGACTAAGATACCAAAAC+TGG | 0.405891 | tig0021904:+6085 | Msa1395000:CDS |
| CCACATGCATTGCATAAAAC+TGG | 0.410323 | tig0021904:-6066 | None:intergenic |
| AGCAACTCCAGCATGGCGCC+AGG | 0.413779 | tig0021904:+6029 | Msa1395000:CDS |
| CTCCAAAGAGGCGTAGTTTG+AGG | 0.425603 | tig0021904:+6399 | Msa1395000:CDS |
| CGAACAATAAGGCCTCTTCT+GGG | 0.435884 | tig0021904:-6502 | None:intergenic |
| CTGGCTTCTCTTCTGGTCCC+TGG | 0.474139 | tig0021904:-6047 | None:intergenic |
| TCTTCTGGGGATGACTCATC+TGG | 0.478086 | tig0021904:-6488 | None:intergenic |
| GAGGATTATCAATCAGAAGA+TGG | 0.496715 | tig0021904:+6418 | Msa1395000:CDS |
| TTCATATTCATGCAAGTCTA+CGG | 0.538240 | tig0021904:+6373 | Msa1395000:CDS |
| ATCTGAATCAGAAACATCAA+TGG | 0.539843 | tig0021904:-6208 | None:intergenic |
| TTCTACATAGAAGAACCGTC+TGG | 0.563851 | tig0021904:-6584 | None:intergenic |
| GCAACTCCAGCATGGCGCCA+GGG | 0.572723 | tig0021904:+6030 | Msa1395000:CDS |
| TTTGGAAAACAACGTTTGAG+AGG | 0.575726 | tig0021904:-6126 | None:intergenic |
| AGATGAGTCATCCCCAGAAG+AGG | 0.591895 | tig0021904:+6490 | Msa1395000:CDS |
| GCAGTTCTTCTTAAACCAGA+CGG | 0.605128 | tig0021904:+6569 | Msa1395000:CDS |
| TGGACTAAGATACCAAAACT+GGG | 0.618651 | tig0021904:+6086 | Msa1395000:CDS |
| GAACAATAAGGCCTCTTCTG+GGG | 0.622845 | tig0021904:-6501 | None:intergenic |
| AAAGAACTTGTTACGAACAG+TGG | 0.644687 | tig0021904:+6147 | Msa1395000:CDS |
| AGTCTACGGTCTCTCCAAAG+AGG | 0.659563 | tig0021904:+6387 | Msa1395000:CDS |
| GGACTAAGATACCAAAACTG+GGG | 0.731002 | tig0021904:+6087 | Msa1395000:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | TGTAAAGAAAAGAGTGAAAA+TGG | - | tig0021904:6261-6280 | None:intergenic | 25.0% |
| ! | TTCATACCTTCAAATGAAAT+TGG | + | tig0021904:6539-6558 | Msa1395000:CDS | 25.0% |
| ATCTGAATCAGAAACATCAA+TGG | - | tig0021904:6211-6230 | None:intergenic | 30.0% | |
| ! | GATGTTTCTGATTCAGATAA+TGG | + | tig0021904:6213-6232 | Msa1395000:CDS | 30.0% |
| TTCATATTCATGCAAGTCTA+CGG | + | tig0021904:6373-6392 | Msa1395000:CDS | 30.0% | |
| ! | GTTCACATTTTCGAACAATA+AGG | - | tig0021904:6516-6535 | None:intergenic | 30.0% |
| TGGACTAAGATACCAAAACT+GGG | + | tig0021904:6086-6105 | Msa1395000:CDS | 35.0% | |
| TTTGGAAAACAACGTTTGAG+AGG | - | tig0021904:6129-6148 | None:intergenic | 35.0% | |
| ! | CTGTTCGTAACAAGTTCTTT+TGG | - | tig0021904:6147-6166 | None:intergenic | 35.0% |
| !! | AAAGAACTTGTTACGAACAG+TGG | + | tig0021904:6147-6166 | Msa1395000:CDS | 35.0% |
| !! | TGCTATATACTACTTTTGCG+AGG | + | tig0021904:6282-6301 | Msa1395000:intron | 35.0% |
| !! | GCTATATACTACTTTTGCGA+GGG | + | tig0021904:6283-6302 | Msa1395000:intron | 35.0% |
| GAGGATTATCAATCAGAAGA+TGG | + | tig0021904:6418-6437 | Msa1395000:CDS | 35.0% | |
| CCAAGACCAATTTCATTTGA+AGG | - | tig0021904:6548-6567 | None:intergenic | 35.0% | |
| ! | CCTTCAAATGAAATTGGTCT+TGG | + | tig0021904:6545-6564 | Msa1395000:CDS | 35.0% |
| ! | CATAAAACTGGCTTCTCTTC+TGG | - | tig0021904:6057-6076 | None:intergenic | 40.0% |
| CCACATGCATTGCATAAAAC+TGG | - | tig0021904:6069-6088 | None:intergenic | 40.0% | |
| !! | CCAGTTTTATGCAATGCATG+TGG | + | tig0021904:6066-6085 | Msa1395000:CDS | 40.0% |
| ! | GTGGACTAAGATACCAAAAC+TGG | + | tig0021904:6085-6104 | Msa1395000:CDS | 40.0% |
| GGACTAAGATACCAAAACTG+GGG | + | tig0021904:6087-6106 | Msa1395000:CDS | 40.0% | |
| ! | CTTCAAGATTTCCCCAGTTT+TGG | - | tig0021904:6101-6120 | None:intergenic | 40.0% |
| !! | ATATACTACTTTTGCGAGGG+TGG | + | tig0021904:6286-6305 | Msa1395000:intron | 40.0% |
| !! | TATACTACTTTTGCGAGGGT+GGG | + | tig0021904:6287-6306 | Msa1395000:intron | 40.0% |
| GCAGTTCTTCTTAAACCAGA+CGG | + | tig0021904:6569-6588 | Msa1395000:CDS | 40.0% | |
| ! | TTTTTCCAGCAACTCCAGCA+TGG | + | tig0021904:6022-6041 | Msa1395000:CDS | 45.0% |
| ATCCTCAAACTACGCCTCTT+TGG | - | tig0021904:6404-6423 | None:intergenic | 45.0% | |
| GAACAATAAGGCCTCTTCTG+GGG | - | tig0021904:6504-6523 | None:intergenic | 45.0% | |
| CGAACAATAAGGCCTCTTCT+GGG | - | tig0021904:6505-6524 | None:intergenic | 45.0% | |
| TCGAACAATAAGGCCTCTTC+TGG | - | tig0021904:6506-6525 | None:intergenic | 45.0% | |
| AGTCTACGGTCTCTCCAAAG+AGG | + | tig0021904:6387-6406 | Msa1395000:CDS | 50.0% | |
| ! | CTCCAAAGAGGCGTAGTTTG+AGG | + | tig0021904:6399-6418 | Msa1395000:CDS | 50.0% |
| ! | TCTTCTGGGGATGACTCATC+TGG | - | tig0021904:6491-6510 | None:intergenic | 50.0% |
| AGATGAGTCATCCCCAGAAG+AGG | + | tig0021904:6490-6509 | Msa1395000:CDS | 50.0% | |
| CTGGCTTCTCTTCTGGTCCC+TGG | - | tig0021904:6050-6069 | None:intergenic | 60.0% | |
| TGGCGCCATGCTGGAGTTGC+TGG | - | tig0021904:6030-6049 | None:intergenic | 65.0% | |
| AGCAACTCCAGCATGGCGCC+AGG | + | tig0021904:6029-6048 | Msa1395000:CDS | 65.0% | |
| GCAACTCCAGCATGGCGCCA+GGG | + | tig0021904:6030-6049 | Msa1395000:CDS | 65.0% | |
| TCTGGTCCCTGGCGCCATGC+TGG | - | tig0021904:6039-6058 | None:intergenic | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| tig0021904 | gene | 6018 | 6604 | 6018 | ID=Msa1395000;Name=Msa1395000 |
| tig0021904 | mRNA | 6018 | 6604 | 6018 | ID=Msa1395000-mRNA-1;Parent=Msa1395000;Name=Msa1395000-mRNA-1;_AED=0.02;_eAED=0.02;_QI=0|0|0|1|1|1|2|0|152 |
| tig0021904 | exon | 6018 | 6234 | 6018 | ID=Msa1395000-mRNA-1:exon:14669;Parent=Msa1395000-mRNA-1 |
| tig0021904 | exon | 6363 | 6604 | 6363 | ID=Msa1395000-mRNA-1:exon:14670;Parent=Msa1395000-mRNA-1 |
| tig0021904 | CDS | 6018 | 6234 | 6018 | ID=Msa1395000-mRNA-1:cds;Parent=Msa1395000-mRNA-1 |
| tig0021904 | CDS | 6363 | 6604 | 6363 | ID=Msa1395000-mRNA-1:cds;Parent=Msa1395000-mRNA-1 |
| Gene Sequence |
| Protein sequence |
