AlfalfaGEDB | Msa1398750

M. sativa cultivar zhongmu-4 / Msa1398750

KEGG: K14491
GO: -
PFAM: PF00072.27

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1398750	AET04690.2	66.667	255	28	4	1	247	1	206	1.27e-105	317

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1398750	sp\|A2XE31\|ORR21_ORYSI	32.979	282	111	2	13	252	11	256	7.95e-41	151

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1398750	G7LD51	66.667	255	28	4	1	247	1	206	6.06e-106	317

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TFs/TRs:

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Gene ID	Type	Classification
Msa1398750	TF	GARP-ARR-B

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
No data available in table

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PPI

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1398750	MtrunA17_Chr8g0383961	64.314	255	24	4	1	247	1	196	5.14e-104	302
Msa1398750	MtrunA17_Chr6g0471041	50.973	257	80	4	1	247	1	221	8.80e-78	241
Msa1398750	MtrunA17_Chr4g0009851	46.512	258	87	5	1	247	1	218	1.27e-67	211
Msa1398750	MtrunA17_Chr4g0009901	43.411	258	94	5	1	247	1	217	1.21e-58	187
Msa1398750	MtrunA17_Chr1g0161431	38.298	282	97	3	12	252	9	254	6.17e-56	189
Msa1398750	MtrunA17_Chr8g0391911	38.745	271	104	5	9	248	4	243	2.04e-49	163
Msa1398750	MtrunA17_Chr4g0009981	43.781	201	66	3	58	247	1	165	2.74e-48	160
Msa1398750	MtrunA17_Chr4g0011071	51.429	140	57	2	61	189	36	175	1.96e-43	145
Msa1398750	MtrunA17_Chr2g0294911	36.496	274	104	3	13	252	28	265	5.35e-43	154
Msa1398750	MtrunA17_Chr6g0456631	47.134	157	70	4	9	153	4	159	2.70e-42	142

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1398750	AT2G01760.1	32.982	285	110	3	13	252	6	254	4.56e-41	145
Msa1398750	AT2G01760.2	30.032	313	110	4	13	252	6	282	8.73e-36	131
Msa1398750	AT1G67710.1	33.696	276	110	5	15	252	8	248	2.28e-35	132
Msa1398750	AT3G16857.1	30.565	301	117	3	8	252	27	291	8.56e-35	132
Msa1398750	AT3G16857.2	30.565	301	117	3	8	252	27	291	9.33e-35	132
Msa1398750	AT2G25180.1	31.469	286	125	5	1	252	1	249	2.96e-33	127
Msa1398750	AT4G31920.1	32.721	272	118	6	1	247	1	232	1.79e-30	119
Msa1398750	AT3G16857.3	28.794	257	91	3	52	252	1	221	1.23e-25	105
Msa1398750	AT2G01760.3	29.778	225	77	3	73	252	1	189	6.02e-24	98.2
Msa1398750	AT5G58080.1	26.374	273	131	4	13	252	13	248	2.36e-23	99.0

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Find 42 sgRNAs with CRISPR-Local

Find 114 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAACATAATCAAAGCAAAAG+GGG	0.630491	tig0022299:-25378	Msa1398750:CDS
AAATCATATGAATCCATGTC+TGG	0.476246	tig0022299:+25699	None:intergenic
AAATCCTCTGGGAATTGGCT+GGG	0.555538	tig0022299:+26022	None:intergenic
AACAAACCTTGATAATTGCT+TGG	0.435234	tig0022299:+25938	None:intergenic
AACAAAGGGCTGTGTTGATG+TGG	0.643059	tig0022299:-25742	Msa1398750:CDS
AATTGCATCGACAATTTCTA+AGG	0.211162	tig0022299:-25293	Msa1398750:CDS
ACAAGAACTCTTAAATCCTC+TGG	0.429240	tig0022299:+26010	None:intergenic
ACATGTGGCAGTGTGTTGCA+AGG	0.672995	tig0022299:-25419	Msa1398750:CDS
ACATTTCCACAACAGCATTA+AGG	0.328612	tig0022299:+25966	None:intergenic
ACTTCCCAGCCAATTCCCAG+AGG	0.715638	tig0022299:-26026	Msa1398750:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
TCTATTCCAAGCAATTATCA+AGG	-	tig0022299:25173-25192	Msa1398750:CDS	30.0%
CAAAAGTTCAAGAATTTCAC+AGG	+	tig0022299:25258-25277	None:intergenic	30.0%
AAATCATATGAATCCATGTC+TGG	+	tig0022299:25421-25440	None:intergenic	30.0%
AGAATCAAATCAAGAACATG+TGG	-	tig0022299:25683-25702	Msa1398750:CDS	30.0%
TATACGATTCTTCTTCGTTA+GGG	+	tig0022299:25791-25810	None:intergenic	30.0%
ATATACGATTCTTCTTCGTT+AGG	+	tig0022299:25792-25811	None:intergenic	30.0%
ACAAAAAACAAACCTGCAAA+TGG	+	tig0022299:26050-26069	None:intergenic	30.0%
TCTATTCCAAGCAATTATCA+AGG	-	tig0022299:25173-25192	Msa1398750:CDS	30.0%
CAAAAGTTCAAGAATTTCAC+AGG	+	tig0022299:25258-25277	None:intergenic	30.0%
AAATCATATGAATCCATGTC+TGG	+	tig0022299:25421-25440	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
tig0022299	gene	25065	26074	25065	ID=Msa1398750;Name=Msa1398750
tig0022299	mRNA	25065	26074	25065	ID=Msa1398750-mRNA-1;Parent=Msa1398750;Name=Msa1398750-mRNA-1;_AED=0.19;_eAED=0.32;_QI=0\|0\|0\|1\|1\|1\|3\|0\|252
tig0022299	exon	25945	26074	25945	ID=Msa1398750-mRNA-1:exon:16826;Parent=Msa1398750-mRNA-1
tig0022299	exon	25658	25810	25658	ID=Msa1398750-mRNA-1:exon:16825;Parent=Msa1398750-mRNA-1
tig0022299	exon	25065	25540	25065	ID=Msa1398750-mRNA-1:exon:16824;Parent=Msa1398750-mRNA-1
tig0022299	CDS	25945	26074	25945	ID=Msa1398750-mRNA-1:cds;Parent=Msa1398750-mRNA-1
tig0022299	CDS	25658	25810	25658	ID=Msa1398750-mRNA-1:cds;Parent=Msa1398750-mRNA-1
tig0022299	CDS	25065	25540	25065	ID=Msa1398750-mRNA-1:cds;Parent=Msa1398750-mRNA-1

Gene Sequence

>Msa1398750

ATGGCTCTTTTTAACGATATAGAGTCACTTCCCAGCCAATTCCCAGAGGATTTAAGAGTTCTTGTGGTTGATCATGACACTGATGTCCTTAATGCTGTTGTGGAAATGTCTATTCCAAGCAATTATCAAGTTACAACATGTTCTAAGGCTTCTTTTGCCTTTAAACTTTTGATGGAAACAAAGGGCTGTGTTGATGTGGTACTAATTGAAGCTCAAATGCCAGACATGGATTCATATGATTTTGTCCGACATGTTACCCAACAAATCAATATTCCTGTCATCATGATGTGTGATGATGGATCAACAAGTGCAGTGATGAAAGCTGTTGCAAACGGGGCTTGCGAATATTGGATTAAGCCTTTAATCGAGAATCAAATCAAGAACATGTGGCAGTGTGTTGCAAGGAAATTTTTCAATGAAAATAAACATAATCAAAGCAAAAGGGGAAGAGATGATAATGCTAATTATCAACCCCTAACGAAGAAGAATCGTATATCATGGACACCTGAATTGCATCGACAATTTCTAAGGGTTGTGAATGAACTTGGCCTTGATAGTAAGATTCTTTTTCCAATAATTTTTATTTTCTCTCTCTTATTTTCTTTGTTTGTATACTTCAAGAAACTAAACCTGTGTAAATTTTTGTCTCTCCCTTATTTTTCAGAGGCAAAGCCAAAAGCAATTCATAAAGCGATGAATGTTCCTGGTTTGACAAAAGAGCAAGTTGCTAGCCATTTGCAGGTTTGTTTTTTGTATTAA

Protein sequence

>Msa1398750

MALFNDIESLPSQFPEDLRVLVVDHDTDVLNAVVEMSIPSNYQVTTCSKASFAFKLLMETKGCVDVVLIEAQMPDMDSYDFVRHVTQQINIPVIMMCDDGSTSAVMKAVANGACEYWIKPLIENQIKNMWQCVARKFFNENKHNQSKRGRDDNANYQPLTKKNRISWTPELHRQFLRVVNELGLDSKILFPIIFIFSLLFSLFVYFKKLNLCKFLSLPYFSEAKPKAIHKAMNVPGLTKEQVASHLQVCFLY*