Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1400310 | XP_003591395.1 | 97.500 | 480 | 12 | 0 | 8 | 487 | 1 | 480 | 0.0 | 976 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1400310 | sp|A0A0M4FMK2|RAM1_PETHY | 29.529 | 403 | 265 | 10 | 85 | 485 | 309 | 694 | 1.33e-44 | 169 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1400310 | G7ICX5 | 97.500 | 480 | 12 | 0 | 8 | 487 | 1 | 480 | 0.0 | 976 |
| Gene ID | Type | Classification |
|---|---|---|
| Msa1400310 | TF | GRAS |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1400310 | MtrunA17_Chr1g0194621 | 96.894 | 483 | 15 | 0 | 5 | 487 | 38 | 520 | 0.0 | 975 |
| Msa1400310 | MtrunA17_Chr7g0270031 | 58.761 | 468 | 172 | 9 | 29 | 487 | 101 | 556 | 0.0 | 525 |
| Msa1400310 | MtrunA17_Chr2g0294461 | 45.951 | 494 | 244 | 10 | 6 | 487 | 105 | 587 | 1.21e-135 | 404 |
| Msa1400310 | MtrunA17_Chr2g0294471 | 44.490 | 490 | 241 | 9 | 20 | 487 | 98 | 578 | 3.74e-127 | 382 |
| Msa1400310 | MtrunA17_Chr4g0057891 | 30.691 | 391 | 244 | 13 | 108 | 485 | 144 | 520 | 2.82e-41 | 154 |
| Msa1400310 | MtrunA17_Chr7g0224271 | 28.967 | 397 | 261 | 11 | 100 | 485 | 287 | 673 | 1.37e-39 | 152 |
| Msa1400310 | MtrunA17_Chr8g0357601 | 29.136 | 405 | 263 | 12 | 92 | 485 | 161 | 552 | 1.88e-38 | 147 |
| Msa1400310 | MtrunA17_Chr3g0110971 | 26.161 | 409 | 256 | 10 | 99 | 486 | 205 | 588 | 2.04e-38 | 147 |
| Msa1400310 | MtrunA17_Chr8g0376381 | 28.328 | 293 | 203 | 4 | 199 | 486 | 233 | 523 | 9.34e-37 | 142 |
| Msa1400310 | MtrunA17_Chr7g0238891 | 28.351 | 388 | 254 | 11 | 108 | 485 | 367 | 740 | 1.91e-34 | 137 |
| Msa1400310 | MtrunA17_Chr2g0306641 | 27.387 | 398 | 249 | 12 | 103 | 485 | 115 | 487 | 2.01e-33 | 132 |
| Msa1400310 | MtrunA17_Chr4g0057291 | 23.590 | 390 | 276 | 10 | 105 | 484 | 284 | 661 | 3.81e-32 | 130 |
| Msa1400310 | MtrunA17_Chr5g0396491 | 26.078 | 464 | 253 | 15 | 91 | 485 | 36 | 478 | 1.59e-30 | 124 |
| Msa1400310 | MtrunA17_Chr4g0031741 | 24.936 | 393 | 257 | 12 | 108 | 484 | 279 | 649 | 2.03e-29 | 122 |
| Msa1400310 | MtrunA17_Chr4g0031711 | 25.879 | 398 | 263 | 11 | 103 | 484 | 351 | 732 | 1.19e-28 | 120 |
| Msa1400310 | MtrunA17_Chr2g0290241 | 25.714 | 350 | 237 | 9 | 146 | 485 | 89 | 425 | 4.20e-28 | 116 |
| Msa1400310 | MtrunA17_Chr4g0031701 | 24.422 | 389 | 264 | 9 | 102 | 475 | 243 | 616 | 1.69e-27 | 116 |
| Msa1400310 | MtrunA17_Chr7g0245601 | 26.558 | 369 | 251 | 11 | 118 | 485 | 443 | 792 | 1.94e-27 | 116 |
| Msa1400310 | MtrunA17_Chr2g0328391 | 24.755 | 408 | 268 | 13 | 97 | 484 | 280 | 668 | 7.86e-27 | 114 |
| Msa1400310 | MtrunA17_Chr3g0101141 | 27.042 | 355 | 226 | 15 | 146 | 485 | 206 | 542 | 2.41e-26 | 112 |
| Msa1400310 | MtrunA17_Chr4g0031721 | 24.936 | 389 | 264 | 12 | 108 | 484 | 311 | 683 | 2.63e-26 | 112 |
| Msa1400310 | MtrunA17_Chr2g0328341 | 24.235 | 392 | 261 | 14 | 108 | 484 | 267 | 637 | 4.29e-26 | 112 |
| Msa1400310 | MtrunA17_Chr4g0038761 | 24.661 | 369 | 262 | 9 | 118 | 485 | 89 | 442 | 1.10e-25 | 109 |
| Msa1400310 | MtrunA17_Chr4g0031731 | 24.235 | 392 | 261 | 11 | 102 | 475 | 243 | 616 | 1.60e-25 | 110 |
| Msa1400310 | MtrunA17_Chr4g0076091 | 24.069 | 349 | 242 | 8 | 146 | 484 | 207 | 542 | 3.81e-24 | 105 |
| Msa1400310 | MtrunA17_Chr2g0328431 | 22.905 | 358 | 251 | 9 | 137 | 484 | 311 | 653 | 3.01e-23 | 103 |
| Msa1400310 | MtrunA17_Chr2g0328381 | 23.864 | 440 | 286 | 15 | 67 | 484 | 228 | 640 | 3.71e-23 | 103 |
| Msa1400310 | MtrunA17_Chr2g0328411 | 22.680 | 388 | 271 | 9 | 108 | 484 | 284 | 653 | 7.14e-23 | 102 |
| Msa1400310 | MtrunA17_Chr5g0445331 | 25.000 | 392 | 263 | 14 | 109 | 485 | 157 | 532 | 1.65e-22 | 100 |
| Msa1400310 | MtrunA17_Chr2g0319091 | 25.256 | 293 | 197 | 9 | 206 | 484 | 294 | 578 | 1.78e-22 | 100 |
| Msa1400310 | MtrunA17_Chr1g0183641 | 25.513 | 341 | 231 | 11 | 147 | 484 | 156 | 476 | 5.61e-22 | 98.6 |
| Msa1400310 | MtrunA17_Chr3g0085681 | 24.685 | 397 | 228 | 11 | 146 | 486 | 56 | 437 | 1.61e-20 | 94.0 |
| Msa1400310 | MtrunA17_Chr5g0447691 | 23.143 | 350 | 246 | 10 | 146 | 485 | 208 | 544 | 2.61e-20 | 94.0 |
| Msa1400310 | MtrunA17_Chr4g0038851 | 32.919 | 161 | 100 | 4 | 329 | 485 | 313 | 469 | 5.36e-20 | 92.4 |
| Msa1400310 | MtrunA17_Chr3g0125811 | 27.526 | 287 | 186 | 9 | 213 | 485 | 292 | 570 | 1.62e-19 | 91.7 |
| Msa1400310 | MtrunA17_Chr1g0159461 | 27.431 | 288 | 183 | 11 | 214 | 485 | 315 | 592 | 6.94e-19 | 89.7 |
| Msa1400310 | MtrunA17_Chr4g0039661 | 22.099 | 362 | 248 | 10 | 137 | 485 | 215 | 555 | 1.18e-18 | 89.0 |
| Msa1400310 | MtrunA17_Chr2g0328371 | 27.660 | 235 | 156 | 9 | 206 | 432 | 13 | 241 | 1.80e-18 | 85.1 |
| Msa1400310 | MtrunA17_Chr2g0328361 | 25.524 | 286 | 187 | 9 | 108 | 384 | 269 | 537 | 5.70e-18 | 87.0 |
| Msa1400310 | MtrunA17_Chr3g0114841 | 22.829 | 403 | 277 | 12 | 103 | 484 | 110 | 499 | 1.41e-17 | 85.5 |
| Msa1400310 | MtrunA17_Chr2g0328401 | 21.763 | 363 | 244 | 9 | 108 | 453 | 285 | 624 | 2.38e-17 | 85.1 |
| Msa1400310 | MtrunA17_Chr3g0085361 | 24.294 | 354 | 204 | 10 | 190 | 486 | 5 | 351 | 3.40e-17 | 82.8 |
| Msa1400310 | MtrunA17_Chr5g0401131 | 23.416 | 363 | 255 | 9 | 138 | 485 | 136 | 490 | 3.66e-17 | 84.0 |
| Msa1400310 | MtrunA17_Chr7g0236851 | 23.684 | 380 | 267 | 11 | 112 | 484 | 293 | 656 | 3.80e-16 | 81.3 |
| Msa1400310 | MtrunA17_Chr4g0012071 | 24.912 | 285 | 196 | 8 | 210 | 485 | 276 | 551 | 6.55e-15 | 77.0 |
| Msa1400310 | MtrunA17_Chr8g0383981 | 24.557 | 395 | 251 | 15 | 106 | 484 | 132 | 495 | 7.46e-15 | 77.0 |
| Msa1400310 | MtrunA17_Chr5g0424131 | 21.975 | 405 | 278 | 11 | 102 | 484 | 110 | 498 | 1.08e-14 | 76.3 |
| Msa1400310 | MtrunA17_Chr7g0230731 | 23.515 | 404 | 270 | 16 | 93 | 485 | 353 | 728 | 5.50e-12 | 68.2 |
| Msa1400310 | MtrunA17_Chr1g0200401 | 23.262 | 374 | 232 | 16 | 146 | 487 | 111 | 461 | 8.06e-12 | 67.4 |
| Msa1400310 | MtrunA17_Chr2g0329591 | 20.548 | 365 | 264 | 9 | 138 | 485 | 96 | 451 | 2.30e-11 | 65.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1400310 | AT3G03450.1 | 27.068 | 399 | 259 | 8 | 99 | 486 | 169 | 546 | 3.62e-43 | 160 |
| Msa1400310 | AT1G66350.1 | 25.907 | 386 | 263 | 7 | 102 | 486 | 144 | 507 | 1.75e-42 | 158 |
| Msa1400310 | AT2G01570.1 | 25.954 | 393 | 266 | 8 | 99 | 486 | 210 | 582 | 3.45e-40 | 153 |
| Msa1400310 | AT5G17490.1 | 27.806 | 392 | 249 | 10 | 104 | 486 | 151 | 517 | 1.23e-39 | 150 |
| Msa1400310 | AT1G14920.1 | 26.768 | 396 | 259 | 9 | 99 | 486 | 158 | 530 | 3.28e-39 | 149 |
| Msa1400310 | AT3G46600.2 | 26.750 | 400 | 243 | 14 | 105 | 485 | 3 | 371 | 5.97e-35 | 134 |
| Msa1400310 | AT2G29065.1 | 26.703 | 367 | 239 | 9 | 136 | 484 | 273 | 627 | 7.08e-35 | 138 |
| Msa1400310 | AT5G59450.1 | 27.250 | 400 | 246 | 14 | 108 | 485 | 222 | 598 | 1.09e-34 | 137 |
| Msa1400310 | AT3G46600.3 | 26.799 | 403 | 245 | 14 | 102 | 485 | 176 | 547 | 1.34e-34 | 136 |
| Msa1400310 | AT3G46600.1 | 26.799 | 403 | 245 | 14 | 102 | 485 | 208 | 579 | 2.40e-34 | 136 |
| Msa1400310 | AT1G63100.2 | 24.935 | 385 | 258 | 10 | 121 | 484 | 279 | 653 | 3.27e-30 | 124 |
| Msa1400310 | AT1G63100.1 | 24.935 | 385 | 258 | 10 | 121 | 484 | 254 | 628 | 4.00e-30 | 124 |
| Msa1400310 | AT3G54220.1 | 27.273 | 374 | 243 | 13 | 118 | 484 | 298 | 649 | 2.54e-29 | 122 |
| Msa1400310 | AT1G07530.1 | 24.505 | 404 | 262 | 11 | 101 | 484 | 384 | 764 | 8.32e-29 | 120 |
| Msa1400310 | AT4G17230.1 | 25.000 | 396 | 262 | 12 | 105 | 485 | 150 | 525 | 5.57e-28 | 117 |
| Msa1400310 | AT4G17230.2 | 25.000 | 396 | 262 | 12 | 105 | 485 | 150 | 525 | 5.57e-28 | 117 |
| Msa1400310 | AT5G41920.1 | 26.221 | 389 | 266 | 12 | 100 | 485 | 30 | 400 | 6.96e-28 | 115 |
| Msa1400310 | AT1G50420.1 | 24.889 | 450 | 252 | 15 | 104 | 485 | 48 | 479 | 4.95e-27 | 114 |
| Msa1400310 | AT2G29060.2 | 25.990 | 404 | 242 | 14 | 109 | 484 | 280 | 654 | 8.43e-27 | 114 |
| Msa1400310 | AT2G29060.1 | 25.990 | 404 | 242 | 14 | 109 | 484 | 317 | 691 | 1.05e-26 | 114 |
| Msa1400310 | AT1G07520.2 | 24.100 | 361 | 237 | 9 | 145 | 484 | 184 | 528 | 3.20e-26 | 112 |
| Msa1400310 | AT1G07520.4 | 24.100 | 361 | 237 | 9 | 145 | 484 | 184 | 528 | 3.20e-26 | 112 |
| Msa1400310 | AT1G07520.3 | 24.586 | 362 | 234 | 10 | 145 | 484 | 349 | 693 | 3.38e-26 | 112 |
| Msa1400310 | AT1G07520.1 | 24.586 | 362 | 234 | 10 | 145 | 484 | 348 | 692 | 3.76e-26 | 112 |
| Msa1400310 | AT1G50600.3 | 25.496 | 353 | 234 | 11 | 146 | 485 | 190 | 526 | 2.35e-24 | 106 |
| Msa1400310 | AT1G50600.2 | 25.496 | 353 | 234 | 11 | 146 | 485 | 265 | 601 | 4.22e-24 | 106 |
| Msa1400310 | AT1G50600.1 | 25.496 | 353 | 234 | 11 | 146 | 485 | 261 | 597 | 4.33e-24 | 106 |
| Msa1400310 | AT1G55580.1 | 25.668 | 374 | 241 | 11 | 146 | 485 | 75 | 445 | 1.96e-23 | 103 |
| Msa1400310 | AT2G37650.1 | 24.679 | 389 | 265 | 9 | 108 | 485 | 342 | 713 | 4.58e-23 | 103 |
| Msa1400310 | AT2G37650.2 | 24.679 | 389 | 265 | 9 | 108 | 485 | 330 | 701 | 4.63e-23 | 103 |
| Msa1400310 | AT2G04890.1 | 24.251 | 367 | 249 | 11 | 130 | 485 | 65 | 413 | 1.26e-22 | 100 |
| Msa1400310 | AT5G48150.1 | 23.499 | 383 | 265 | 10 | 118 | 485 | 121 | 490 | 7.20e-22 | 99.0 |
| Msa1400310 | AT5G48150.4 | 23.499 | 383 | 265 | 10 | 118 | 485 | 121 | 490 | 7.20e-22 | 99.0 |
| Msa1400310 | AT5G48150.2 | 23.499 | 383 | 265 | 10 | 118 | 485 | 121 | 490 | 7.20e-22 | 99.0 |
| Msa1400310 | AT5G48150.3 | 23.499 | 383 | 265 | 10 | 118 | 485 | 121 | 490 | 7.20e-22 | 99.0 |
| Msa1400310 | AT4G37650.1 | 22.821 | 390 | 276 | 8 | 114 | 485 | 147 | 529 | 7.78e-21 | 95.9 |
| Msa1400310 | AT5G66770.1 | 22.380 | 353 | 245 | 10 | 145 | 485 | 249 | 584 | 1.58e-17 | 85.9 |
| Msa1400310 | AT3G50650.1 | 22.526 | 293 | 213 | 7 | 206 | 485 | 251 | 542 | 3.20e-15 | 78.6 |
| Msa1400310 | AT3G49950.1 | 24.749 | 299 | 199 | 8 | 206 | 484 | 115 | 407 | 1.51e-14 | 75.9 |
| Msa1400310 | AT4G08250.1 | 24.519 | 416 | 275 | 17 | 87 | 484 | 82 | 476 | 2.77e-14 | 75.5 |
| Msa1400310 | AT4G36710.1 | 22.946 | 353 | 237 | 15 | 146 | 486 | 157 | 486 | 1.58e-11 | 67.0 |
| Msa1400310 | AT1G21450.1 | 23.183 | 289 | 197 | 9 | 213 | 485 | 314 | 593 | 2.33e-11 | 66.6 |
Find 68 sgRNAs with CRISPR-Local
Find 158 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AATGGTAAGTGTTTAATAAT+TGG | 0.167586 | tig0022428:-96803 | Msa1400310:CDS |
| CATTTGATTCATCTTAATAT+TGG | 0.236657 | tig0022428:-97508 | Msa1400310:CDS |
| GACGGAATTGATTGATAAAA+TGG | 0.247556 | tig0022428:-97644 | Msa1400310:CDS |
| AATAATCTTTACATTCCTTA+TGG | 0.249550 | tig0022428:-97940 | Msa1400310:CDS |
| CATGCCACAGTGATGAGTTT+TGG | 0.263501 | tig0022428:+97965 | None:intergenic |
| ATTCCTTATGGAACTGTTCT+TGG | 0.265945 | tig0022428:-97928 | Msa1400310:CDS |
| TCGCAAACAAGGGTGAATTA+TGG | 0.302490 | tig0022428:+97122 | None:intergenic |
| AAGGCTAAGTTAGAAGAAAT+TGG | 0.303167 | tig0022428:-97388 | Msa1400310:CDS |
| ATGAATGAAAGTGGAAATAT+TGG | 0.333082 | tig0022428:-97781 | Msa1400310:CDS |
| TCCATGTTCACTTAGGACTA+TGG | 0.336208 | tig0022428:-97233 | Msa1400310:CDS |
| GATAAGAAACATTGTTGGTT+TGG | 0.346018 | tig0022428:-96987 | Msa1400310:CDS |
| ATTCTGAAGATCACAGCTAT+TGG | 0.354564 | tig0022428:-97421 | Msa1400310:CDS |
| AATCAAGTAATGCAATTTGC+TGG | 0.354696 | tig0022428:-97574 | Msa1400310:CDS |
| TTATGAGTGTAGAGTGATAA+TGG | 0.355610 | tig0022428:-97029 | Msa1400310:CDS |
| GAAGATATAATAACACTTGC+AGG | 0.358002 | tig0022428:-98000 | Msa1400310:CDS |
| ATTCACCCTTGTTTGCGAAT+AGG | 0.360715 | tig0022428:-97117 | Msa1400310:CDS |
| CTTGAAGTGCTTGAGAGAAA+TGG | 0.363368 | tig0022428:+97734 | None:intergenic |
| CAAGCTGAGACTGAATGAAT+TGG | 0.383878 | tig0022428:+96767 | None:intergenic |
| AGGTATAGAATGGTAGAAAC+TGG | 0.390006 | tig0022428:-96905 | Msa1400310:CDS |
| GGAATGTGAAGATTGATGTT+TGG | 0.392726 | tig0022428:-96943 | Msa1400310:CDS |
| TCAAGTACTATCATAATAAA+CGG | 0.431397 | tig0022428:+97157 | None:intergenic |
| CGGACGAGGAAAGATGAAAC+TGG | 0.435732 | tig0022428:-97692 | Msa1400310:CDS |
| TTTCATCTCATGAATGAAAG+TGG | 0.437460 | tig0022428:-97790 | Msa1400310:CDS |
| CAGTTTCATCTTTCCTCGTC+CGG | 0.439195 | tig0022428:+97693 | None:intergenic |
| GAGAGAATCAACAGAGAAAC+CGG | 0.442776 | tig0022428:-97712 | Msa1400310:CDS |
| CAATATTAAGATGAATCAAA+TGG | 0.442889 | tig0022428:+97509 | None:intergenic |
| AATTGCATTACTTGATTGAA+AGG | 0.446453 | tig0022428:+97580 | None:intergenic |
| ATTCATCTTAATATTGGATG+TGG | 0.448243 | tig0022428:-97502 | Msa1400310:CDS |
| TTTATTCTCCATGTTCACTT+AGG | 0.457172 | tig0022428:-97240 | Msa1400310:CDS |
| TGTTGATACTTGTATAAAAG+AGG | 0.464976 | tig0022428:-97053 | Msa1400310:CDS |
| GGTATCAAATTCTGATGGCT+TGG | 0.468081 | tig0022428:-97212 | Msa1400310:CDS |
| GTGATCTTCAGAATTTCAAC+CGG | 0.468897 | tig0022428:+97430 | None:intergenic |
| ATCAATTCCACGGCTTAAGC+AGG | 0.469908 | tig0022428:-98148 | Msa1400310:CDS |
| TGCTGGTGTACAAGCTATAG+TGG | 0.474092 | tig0022428:-97557 | Msa1400310:CDS |
| GAAGGGATAAGAAACATTGT+TGG | 0.474796 | tig0022428:-96992 | Msa1400310:CDS |
| TGTTCTTGGAAGCTTGTTGT+CGG | 0.475836 | tig0022428:-97914 | Msa1400310:CDS |
| GTTCTTTGCAAGGTATAGAA+TGG | 0.484270 | tig0022428:-96915 | Msa1400310:CDS |
| AAGATGAAGAAAGGAAGGTT+AGG | 0.502253 | tig0022428:-96964 | Msa1400310:CDS |
| TGTTTAATAATTGGATGGAA+GGG | 0.503361 | tig0022428:-96794 | Msa1400310:CDS |
| CAATTCATTCAGTCTCAGCT+TGG | 0.509421 | tig0022428:-96766 | Msa1400310:CDS |
| TTGGTTTGGAAGATGAAGAA+AGG | 0.515913 | tig0022428:-96973 | Msa1400310:CDS |
| GTGTTTAATAATTGGATGGA+AGG | 0.523650 | tig0022428:-96795 | Msa1400310:CDS |
| GTTTAATAATTGGATGGAAG+GGG | 0.527241 | tig0022428:-96793 | Msa1400310:CDS |
| TTTGGAAGATGAAGAAAGGA+AGG | 0.529462 | tig0022428:-96969 | Msa1400310:CDS |
| CTTCCAAGAACAGTTCCATA+AGG | 0.531300 | tig0022428:+97925 | None:intergenic |
| AACAAACCTATTCGCAAACA+AGG | 0.532041 | tig0022428:+97111 | None:intergenic |
| GTAAGTGTTTAATAATTGGA+TGG | 0.532866 | tig0022428:-96799 | Msa1400310:CDS |
| AAGCCGTGGAATTGATATCA+TGG | 0.533713 | tig0022428:+98155 | None:intergenic |
| TCAAGTGATGCTAAAGAAGA+TGG | 0.542006 | tig0022428:+98111 | None:intergenic |
| TTTGGAGAAGGTTCTTTGCA+AGG | 0.543910 | tig0022428:-96925 | Msa1400310:CDS |
| ATGGAAGCAATATTATCTGA+AGG | 0.550756 | tig0022428:-97010 | Msa1400310:CDS |
| AGTAGACCGCAAGGAAAAGC+CGG | 0.553680 | tig0022428:-97449 | Msa1400310:CDS |
| CTAGCAGCAGCTGAGAGAGT+AGG | 0.586422 | tig0022428:-97853 | Msa1400310:CDS |
| ACCATAGTCCTAAGTGAACA+TGG | 0.589256 | tig0022428:+97232 | None:intergenic |
| TGGAAGCAATATTATCTGAA+GGG | 0.593391 | tig0022428:-97009 | Msa1400310:CDS |
| TGAAGATTGATGTTTGGAGA+AGG | 0.596614 | tig0022428:-96937 | Msa1400310:CDS |
| TGGCTTGGAGAATTTGATGA+AGG | 0.603211 | tig0022428:-97197 | Msa1400310:CDS |
| ACTATGGTATCAAATTCTGA+TGG | 0.608580 | tig0022428:-97217 | Msa1400310:CDS |
| AAATGAGAGAAACAAGGAGA+CGG | 0.612044 | tig0022428:-97662 | Msa1400310:CDS |
| ATCAACAGAGAAACCGGACG+AGG | 0.613864 | tig0022428:-97706 | Msa1400310:CDS |
| AAGCCATGATATCAATTCCA+CGG | 0.616214 | tig0022428:-98158 | Msa1400310:CDS |
| ATTTGCACCTGCTTAAGCCG+TGG | 0.626082 | tig0022428:+98141 | None:intergenic |
| GCAAGCATTAGTAGACCGCA+AGG | 0.627501 | tig0022428:-97458 | Msa1400310:CDS |
| ACAAACCTATTCGCAAACAA+GGG | 0.673185 | tig0022428:+97112 | None:intergenic |
| TAGATCAAATGAGAGAAACA+AGG | 0.680410 | tig0022428:-97668 | Msa1400310:CDS |
| GATGCTAAAGAAGATGGTGG+TGG | 0.682167 | tig0022428:+98117 | None:intergenic |
| AGTGATGCTAAAGAAGATGG+TGG | 0.725977 | tig0022428:+98114 | None:intergenic |
| ATATCCAAAACTCATCACTG+TGG | 0.776374 | tig0022428:-97969 | Msa1400310:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAAACTATGTTATATAAAAA+GGG | + | tig0022428:97624-97643 | None:intergenic | 10.0% |
| !! | AAAAACTATGTTATATAAAA+AGG | + | tig0022428:97625-97644 | None:intergenic | 10.0% |
| !! | AAAACTATGTTATATAAAAA+GGG | + | tig0022428:97624-97643 | None:intergenic | 10.0% |
| !! | AAAAACTATGTTATATAAAA+AGG | + | tig0022428:97625-97644 | None:intergenic | 10.0% |
| !! | AATAATCTTTACATTCCTTA+TGG | - | tig0022428:97009-97028 | Msa1400310:CDS | 20.0% |
| !! | CAATATTAAGATGAATCAAA+TGG | + | tig0022428:97443-97462 | None:intergenic | 20.0% |
| !! | CATTTGATTCATCTTAATAT+TGG | - | tig0022428:97441-97460 | Msa1400310:CDS | 20.0% |
| !! | TCAAGTACTATCATAATAAA+CGG | + | tig0022428:97795-97814 | None:intergenic | 20.0% |
| !!! | TTTTGTACTATAGACAAAAA+TGG | - | tig0022428:98128-98147 | Msa1400310:CDS | 20.0% |
| !! | AATGGTAAGTGTTTAATAAT+TGG | - | tig0022428:98146-98165 | Msa1400310:CDS | 20.0% |
| !! | AATAATCTTTACATTCCTTA+TGG | - | tig0022428:97009-97028 | Msa1400310:CDS | 20.0% |
| !! | CAATATTAAGATGAATCAAA+TGG | + | tig0022428:97443-97462 | None:intergenic | 20.0% |
| !! | CATTTGATTCATCTTAATAT+TGG | - | tig0022428:97441-97460 | Msa1400310:CDS | 20.0% |
| !! | TCAAGTACTATCATAATAAA+CGG | + | tig0022428:97795-97814 | None:intergenic | 20.0% |
| !!! | TTTTGTACTATAGACAAAAA+TGG | - | tig0022428:98128-98147 | Msa1400310:CDS | 20.0% |
| !! | AATGGTAAGTGTTTAATAAT+TGG | - | tig0022428:98146-98165 | Msa1400310:CDS | 20.0% |
| ! | CTTGAGCTATTGAAAAATTA+TGG | - | tig0022428:96856-96875 | Msa1400310:CDS | 25.0% |
| ! | ATGAATGAAAGTGGAAATAT+TGG | - | tig0022428:97168-97187 | Msa1400310:CDS | 25.0% |
| ! | AATTGCATTACTTGATTGAA+AGG | + | tig0022428:97372-97391 | None:intergenic | 25.0% |
| ! | ATTCATCTTAATATTGGATG+TGG | - | tig0022428:97447-97466 | Msa1400310:CDS | 25.0% |
| !! | ATATAAAAAGGGTATGTTCA+AGG | + | tig0022428:97613-97632 | None:intergenic | 25.0% |
| ! | TGTTGATACTTGTATAAAAG+AGG | - | tig0022428:97896-97915 | Msa1400310:CDS | 25.0% |
| ! | TTACTAAAAAGTTTGACTGT+GGG | - | tig0022428:98102-98121 | Msa1400310:CDS | 25.0% |
| ! | GTAAGTGTTTAATAATTGGA+TGG | - | tig0022428:98150-98169 | Msa1400310:CDS | 25.0% |
| ! | TGTTTAATAATTGGATGGAA+GGG | - | tig0022428:98155-98174 | Msa1400310:CDS | 25.0% |
| ! | CTTGAGCTATTGAAAAATTA+TGG | - | tig0022428:96856-96875 | Msa1400310:CDS | 25.0% |
| ! | ATGAATGAAAGTGGAAATAT+TGG | - | tig0022428:97168-97187 | Msa1400310:CDS | 25.0% |
| ! | AATTGCATTACTTGATTGAA+AGG | + | tig0022428:97372-97391 | None:intergenic | 25.0% |
| ! | ATTCATCTTAATATTGGATG+TGG | - | tig0022428:97447-97466 | Msa1400310:CDS | 25.0% |
| !! | ATATAAAAAGGGTATGTTCA+AGG | + | tig0022428:97613-97632 | None:intergenic | 25.0% |
| ! | TGTTGATACTTGTATAAAAG+AGG | - | tig0022428:97896-97915 | Msa1400310:CDS | 25.0% |
| ! | TTACTAAAAAGTTTGACTGT+GGG | - | tig0022428:98102-98121 | Msa1400310:CDS | 25.0% |
| ! | GTAAGTGTTTAATAATTGGA+TGG | - | tig0022428:98150-98169 | Msa1400310:CDS | 25.0% |
| ! | TGTTTAATAATTGGATGGAA+GGG | - | tig0022428:98155-98174 | Msa1400310:CDS | 25.0% |
| GAAGATATAATAACACTTGC+AGG | - | tig0022428:96949-96968 | Msa1400310:CDS | 30.0% | |
| TTTCATCTCATGAATGAAAG+TGG | - | tig0022428:97159-97178 | Msa1400310:CDS | 30.0% | |
| TAGATCAAATGAGAGAAACA+AGG | - | tig0022428:97281-97300 | Msa1400310:CDS | 30.0% | |
| GACGGAATTGATTGATAAAA+TGG | - | tig0022428:97305-97324 | Msa1400310:CDS | 30.0% | |
| AATCAAGTAATGCAATTTGC+TGG | - | tig0022428:97375-97394 | Msa1400310:CDS | 30.0% | |
| ! | AGCTATTGGATTTTCTAACA+AGG | - | tig0022428:97542-97561 | Msa1400310:CDS | 30.0% |
| AAGGCTAAGTTAGAAGAAAT+TGG | - | tig0022428:97561-97580 | Msa1400310:CDS | 30.0% | |
| ! | AGTTTTTGTTGAAGATGCTA+TGG | - | tig0022428:97638-97657 | Msa1400310:CDS | 30.0% |
| TTTATTCTCCATGTTCACTT+AGG | - | tig0022428:97709-97728 | Msa1400310:CDS | 30.0% | |
| ACTATGGTATCAAATTCTGA+TGG | - | tig0022428:97732-97751 | Msa1400310:CDS | 30.0% | |
| TTATGAGTGTAGAGTGATAA+TGG | - | tig0022428:97920-97939 | Msa1400310:CDS | 30.0% | |
| ATGGAAGCAATATTATCTGA+AGG | - | tig0022428:97939-97958 | Msa1400310:CDS | 30.0% | |
| TGGAAGCAATATTATCTGAA+GGG | - | tig0022428:97940-97959 | Msa1400310:CDS | 30.0% | |
| ! | GATAAGAAACATTGTTGGTT+TGG | - | tig0022428:97962-97981 | Msa1400310:CDS | 30.0% |
| GTTACTAAAAAGTTTGACTG+TGG | - | tig0022428:98101-98120 | Msa1400310:CDS | 30.0% | |
| GTGTTTAATAATTGGATGGA+AGG | - | tig0022428:98154-98173 | Msa1400310:CDS | 30.0% | |
| GTTTAATAATTGGATGGAAG+GGG | - | tig0022428:98156-98175 | Msa1400310:CDS | 30.0% | |
| GAAGATATAATAACACTTGC+AGG | - | tig0022428:96949-96968 | Msa1400310:CDS | 30.0% | |
| TTTCATCTCATGAATGAAAG+TGG | - | tig0022428:97159-97178 | Msa1400310:CDS | 30.0% | |
| TAGATCAAATGAGAGAAACA+AGG | - | tig0022428:97281-97300 | Msa1400310:CDS | 30.0% | |
| GACGGAATTGATTGATAAAA+TGG | - | tig0022428:97305-97324 | Msa1400310:CDS | 30.0% | |
| AATCAAGTAATGCAATTTGC+TGG | - | tig0022428:97375-97394 | Msa1400310:CDS | 30.0% | |
| ! | AGCTATTGGATTTTCTAACA+AGG | - | tig0022428:97542-97561 | Msa1400310:CDS | 30.0% |
| AAGGCTAAGTTAGAAGAAAT+TGG | - | tig0022428:97561-97580 | Msa1400310:CDS | 30.0% | |
| ! | AGTTTTTGTTGAAGATGCTA+TGG | - | tig0022428:97638-97657 | Msa1400310:CDS | 30.0% |
| TTTATTCTCCATGTTCACTT+AGG | - | tig0022428:97709-97728 | Msa1400310:CDS | 30.0% | |
| ACTATGGTATCAAATTCTGA+TGG | - | tig0022428:97732-97751 | Msa1400310:CDS | 30.0% | |
| TTATGAGTGTAGAGTGATAA+TGG | - | tig0022428:97920-97939 | Msa1400310:CDS | 30.0% | |
| ATGGAAGCAATATTATCTGA+AGG | - | tig0022428:97939-97958 | Msa1400310:CDS | 30.0% | |
| TGGAAGCAATATTATCTGAA+GGG | - | tig0022428:97940-97959 | Msa1400310:CDS | 30.0% | |
| ! | GATAAGAAACATTGTTGGTT+TGG | - | tig0022428:97962-97981 | Msa1400310:CDS | 30.0% |
| GTTACTAAAAAGTTTGACTG+TGG | - | tig0022428:98101-98120 | Msa1400310:CDS | 30.0% | |
| GTGTTTAATAATTGGATGGA+AGG | - | tig0022428:98154-98173 | Msa1400310:CDS | 30.0% | |
| GTTTAATAATTGGATGGAAG+GGG | - | tig0022428:98156-98175 | Msa1400310:CDS | 30.0% | |
| AAGCCATGATATCAATTCCA+CGG | - | tig0022428:96791-96810 | Msa1400310:CDS | 35.0% | |
| TCAAGTGATGCTAAAGAAGA+TGG | + | tig0022428:96841-96860 | None:intergenic | 35.0% | |
| ! | TCTTCAGTTGACAACTTTTG+TGG | + | tig0022428:96934-96953 | None:intergenic | 35.0% |
| ATATCCAAAACTCATCACTG+TGG | - | tig0022428:96980-96999 | Msa1400310:CDS | 35.0% | |
| ATTCCTTATGGAACTGTTCT+TGG | - | tig0022428:97021-97040 | Msa1400310:CDS | 35.0% | |
| AAATGAGAGAAACAAGGAGA+CGG | - | tig0022428:97287-97306 | Msa1400310:CDS | 35.0% | |
| GTGATCTTCAGAATTTCAAC+CGG | + | tig0022428:97522-97541 | None:intergenic | 35.0% | |
| ! | ATTCTGAAGATCACAGCTAT+TGG | - | tig0022428:97528-97547 | Msa1400310:CDS | 35.0% |
| ACAAACCTATTCGCAAACAA+GGG | + | tig0022428:97840-97859 | None:intergenic | 35.0% | |
| AACAAACCTATTCGCAAACA+AGG | + | tig0022428:97841-97860 | None:intergenic | 35.0% | |
| GAAGGGATAAGAAACATTGT+TGG | - | tig0022428:97957-97976 | Msa1400310:CDS | 35.0% | |
| !! | TTGGTTTGGAAGATGAAGAA+AGG | - | tig0022428:97976-97995 | Msa1400310:CDS | 35.0% |
| TTTGGAAGATGAAGAAAGGA+AGG | - | tig0022428:97980-97999 | Msa1400310:CDS | 35.0% | |
| AAGATGAAGAAAGGAAGGTT+AGG | - | tig0022428:97985-98004 | Msa1400310:CDS | 35.0% | |
| GGAATGTGAAGATTGATGTT+TGG | - | tig0022428:98006-98025 | Msa1400310:CDS | 35.0% | |
| TGAAGATTGATGTTTGGAGA+AGG | - | tig0022428:98012-98031 | Msa1400310:CDS | 35.0% | |
| GTTCTTTGCAAGGTATAGAA+TGG | - | tig0022428:98034-98053 | Msa1400310:CDS | 35.0% | |
| AGGTATAGAATGGTAGAAAC+TGG | - | tig0022428:98044-98063 | Msa1400310:CDS | 35.0% | |
| AAGCCATGATATCAATTCCA+CGG | - | tig0022428:96791-96810 | Msa1400310:CDS | 35.0% | |
| TCAAGTGATGCTAAAGAAGA+TGG | + | tig0022428:96841-96860 | None:intergenic | 35.0% | |
| ! | TCTTCAGTTGACAACTTTTG+TGG | + | tig0022428:96934-96953 | None:intergenic | 35.0% |
| ATATCCAAAACTCATCACTG+TGG | - | tig0022428:96980-96999 | Msa1400310:CDS | 35.0% | |
| ATTCCTTATGGAACTGTTCT+TGG | - | tig0022428:97021-97040 | Msa1400310:CDS | 35.0% | |
| AAATGAGAGAAACAAGGAGA+CGG | - | tig0022428:97287-97306 | Msa1400310:CDS | 35.0% | |
| GTGATCTTCAGAATTTCAAC+CGG | + | tig0022428:97522-97541 | None:intergenic | 35.0% | |
| ! | ATTCTGAAGATCACAGCTAT+TGG | - | tig0022428:97528-97547 | Msa1400310:CDS | 35.0% |
| ACAAACCTATTCGCAAACAA+GGG | + | tig0022428:97840-97859 | None:intergenic | 35.0% | |
| AACAAACCTATTCGCAAACA+AGG | + | tig0022428:97841-97860 | None:intergenic | 35.0% | |
| GAAGGGATAAGAAACATTGT+TGG | - | tig0022428:97957-97976 | Msa1400310:CDS | 35.0% | |
| !! | TTGGTTTGGAAGATGAAGAA+AGG | - | tig0022428:97976-97995 | Msa1400310:CDS | 35.0% |
| TTTGGAAGATGAAGAAAGGA+AGG | - | tig0022428:97980-97999 | Msa1400310:CDS | 35.0% | |
| AAGATGAAGAAAGGAAGGTT+AGG | - | tig0022428:97985-98004 | Msa1400310:CDS | 35.0% | |
| GGAATGTGAAGATTGATGTT+TGG | - | tig0022428:98006-98025 | Msa1400310:CDS | 35.0% | |
| TGAAGATTGATGTTTGGAGA+AGG | - | tig0022428:98012-98031 | Msa1400310:CDS | 35.0% | |
| GTTCTTTGCAAGGTATAGAA+TGG | - | tig0022428:98034-98053 | Msa1400310:CDS | 35.0% | |
| AGGTATAGAATGGTAGAAAC+TGG | - | tig0022428:98044-98063 | Msa1400310:CDS | 35.0% | |
| AAGCCGTGGAATTGATATCA+TGG | + | tig0022428:96797-96816 | None:intergenic | 40.0% | |
| AGTGATGCTAAAGAAGATGG+TGG | + | tig0022428:96838-96857 | None:intergenic | 40.0% | |
| CTTCCAAGAACAGTTCCATA+AGG | + | tig0022428:97027-97046 | None:intergenic | 40.0% | |
| !! | TGTTCTTGGAAGCTTGTTGT+CGG | - | tig0022428:97035-97054 | Msa1400310:CDS | 40.0% |
| ! | CTTGAAGTGCTTGAGAGAAA+TGG | + | tig0022428:97218-97237 | None:intergenic | 40.0% |
| GAGAGAATCAACAGAGAAAC+CGG | - | tig0022428:97237-97256 | Msa1400310:CDS | 40.0% | |
| TCCATGTTCACTTAGGACTA+TGG | - | tig0022428:97716-97735 | Msa1400310:CDS | 40.0% | |
| ACCATAGTCCTAAGTGAACA+TGG | + | tig0022428:97720-97739 | None:intergenic | 40.0% | |
| GGTATCAAATTCTGATGGCT+TGG | - | tig0022428:97737-97756 | Msa1400310:CDS | 40.0% | |
| ! | TGGCTTGGAGAATTTGATGA+AGG | - | tig0022428:97752-97771 | Msa1400310:CDS | 40.0% |
| TCGCAAACAAGGGTGAATTA+TGG | + | tig0022428:97830-97849 | None:intergenic | 40.0% | |
| ! | ATTCACCCTTGTTTGCGAAT+AGG | - | tig0022428:97832-97851 | Msa1400310:CDS | 40.0% |
| TTTGGAGAAGGTTCTTTGCA+AGG | - | tig0022428:98024-98043 | Msa1400310:CDS | 40.0% | |
| CAAGCTGAGACTGAATGAAT+TGG | + | tig0022428:98185-98204 | None:intergenic | 40.0% | |
| CAATTCATTCAGTCTCAGCT+TGG | - | tig0022428:98183-98202 | Msa1400310:CDS | 40.0% | |
| AAGCCGTGGAATTGATATCA+TGG | + | tig0022428:96797-96816 | None:intergenic | 40.0% | |
| AGTGATGCTAAAGAAGATGG+TGG | + | tig0022428:96838-96857 | None:intergenic | 40.0% | |
| CTTCCAAGAACAGTTCCATA+AGG | + | tig0022428:97027-97046 | None:intergenic | 40.0% | |
| !! | TGTTCTTGGAAGCTTGTTGT+CGG | - | tig0022428:97035-97054 | Msa1400310:CDS | 40.0% |
| ! | CTTGAAGTGCTTGAGAGAAA+TGG | + | tig0022428:97218-97237 | None:intergenic | 40.0% |
| GAGAGAATCAACAGAGAAAC+CGG | - | tig0022428:97237-97256 | Msa1400310:CDS | 40.0% | |
| TCCATGTTCACTTAGGACTA+TGG | - | tig0022428:97716-97735 | Msa1400310:CDS | 40.0% | |
| ACCATAGTCCTAAGTGAACA+TGG | + | tig0022428:97720-97739 | None:intergenic | 40.0% | |
| GGTATCAAATTCTGATGGCT+TGG | - | tig0022428:97737-97756 | Msa1400310:CDS | 40.0% | |
| ! | TGGCTTGGAGAATTTGATGA+AGG | - | tig0022428:97752-97771 | Msa1400310:CDS | 40.0% |
| TCGCAAACAAGGGTGAATTA+TGG | + | tig0022428:97830-97849 | None:intergenic | 40.0% | |
| ! | ATTCACCCTTGTTTGCGAAT+AGG | - | tig0022428:97832-97851 | Msa1400310:CDS | 40.0% |
| TTTGGAGAAGGTTCTTTGCA+AGG | - | tig0022428:98024-98043 | Msa1400310:CDS | 40.0% | |
| CAAGCTGAGACTGAATGAAT+TGG | + | tig0022428:98185-98204 | None:intergenic | 40.0% | |
| CAATTCATTCAGTCTCAGCT+TGG | - | tig0022428:98183-98202 | Msa1400310:CDS | 40.0% | |
| ATCAATTCCACGGCTTAAGC+AGG | - | tig0022428:96801-96820 | Msa1400310:CDS | 45.0% | |
| ! | GATGCTAAAGAAGATGGTGG+TGG | + | tig0022428:96835-96854 | None:intergenic | 45.0% |
| ! | CATGCCACAGTGATGAGTTT+TGG | + | tig0022428:96987-97006 | None:intergenic | 45.0% |
| CAGTTTCATCTTTCCTCGTC+CGG | + | tig0022428:97259-97278 | None:intergenic | 45.0% | |
| TGCTGGTGTACAAGCTATAG+TGG | - | tig0022428:97392-97411 | Msa1400310:CDS | 45.0% | |
| !! | TTTCAACCGGCTTTTCCTTG+CGG | + | tig0022428:97509-97528 | None:intergenic | 45.0% |
| ATCAATTCCACGGCTTAAGC+AGG | - | tig0022428:96801-96820 | Msa1400310:CDS | 45.0% | |
| ! | GATGCTAAAGAAGATGGTGG+TGG | + | tig0022428:96835-96854 | None:intergenic | 45.0% |
| ! | CATGCCACAGTGATGAGTTT+TGG | + | tig0022428:96987-97006 | None:intergenic | 45.0% |
| CAGTTTCATCTTTCCTCGTC+CGG | + | tig0022428:97259-97278 | None:intergenic | 45.0% | |
| TGCTGGTGTACAAGCTATAG+TGG | - | tig0022428:97392-97411 | Msa1400310:CDS | 45.0% | |
| !! | TTTCAACCGGCTTTTCCTTG+CGG | + | tig0022428:97509-97528 | None:intergenic | 45.0% |
| ATTTGCACCTGCTTAAGCCG+TGG | + | tig0022428:96811-96830 | None:intergenic | 50.0% | |
| ATCAACAGAGAAACCGGACG+AGG | - | tig0022428:97243-97262 | Msa1400310:CDS | 50.0% | |
| CGGACGAGGAAAGATGAAAC+TGG | - | tig0022428:97257-97276 | Msa1400310:CDS | 50.0% | |
| GCAAGCATTAGTAGACCGCA+AGG | - | tig0022428:97491-97510 | Msa1400310:CDS | 50.0% | |
| AGTAGACCGCAAGGAAAAGC+CGG | - | tig0022428:97500-97519 | Msa1400310:CDS | 50.0% | |
| ATTTGCACCTGCTTAAGCCG+TGG | + | tig0022428:96811-96830 | None:intergenic | 50.0% | |
| ATCAACAGAGAAACCGGACG+AGG | - | tig0022428:97243-97262 | Msa1400310:CDS | 50.0% | |
| CGGACGAGGAAAGATGAAAC+TGG | - | tig0022428:97257-97276 | Msa1400310:CDS | 50.0% | |
| GCAAGCATTAGTAGACCGCA+AGG | - | tig0022428:97491-97510 | Msa1400310:CDS | 50.0% | |
| AGTAGACCGCAAGGAAAAGC+CGG | - | tig0022428:97500-97519 | Msa1400310:CDS | 50.0% | |
| CTAGCAGCAGCTGAGAGAGT+AGG | - | tig0022428:97096-97115 | Msa1400310:CDS | 55.0% | |
| CTAGCAGCAGCTGAGAGAGT+AGG | - | tig0022428:97096-97115 | Msa1400310:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| tig0022428 | gene | 96754 | 98217 | 96754 | ID=Msa1400310;Name=Msa1400310 |
| tig0022428 | mRNA | 96754 | 98217 | 96754 | ID=Msa1400310-mRNA-1;Parent=Msa1400310;Name=Msa1400310-mRNA-1;_AED=0.01;_eAED=0.01;_QI=0|-1|0|1|-1|1|1|0|487 |
| tig0022428 | exon | 96754 | 98217 | 96754 | ID=Msa1400310-mRNA-1:exon:17669;Parent=Msa1400310-mRNA-1 |
| tig0022428 | CDS | 96754 | 98217 | 96754 | ID=Msa1400310-mRNA-1:cds;Parent=Msa1400310-mRNA-1 |
| Gene Sequence |
| Protein sequence |
