AlfalfaGEDB | Msa1400310

M. sativa cultivar zhongmu-4 / Msa1400310

KEGG: K14494
GO: -
PFAM: PF03514.17

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1400310	XP_003591395.1	97.500	480	12	0	8	487	1	480	0.0	976

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1400310	sp\|A0A0M4FMK2\|RAM1_PETHY	29.529	403	265	10	85	485	309	694	1.33e-44	169

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1400310	G7ICX5	97.500	480	12	0	8	487	1	480	0.0	976

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TFs/TRs:

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Gene ID	Type	Classification
Msa1400310	TF	GRAS

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1400310	MtrunA17_Chr1g0194621	96.894	483	15	0	5	487	38	520	0.0	975
Msa1400310	MtrunA17_Chr7g0270031	58.761	468	172	9	29	487	101	556	0.0	525
Msa1400310	MtrunA17_Chr2g0294461	45.951	494	244	10	6	487	105	587	1.21e-135	404
Msa1400310	MtrunA17_Chr2g0294471	44.490	490	241	9	20	487	98	578	3.74e-127	382
Msa1400310	MtrunA17_Chr4g0057891	30.691	391	244	13	108	485	144	520	2.82e-41	154
Msa1400310	MtrunA17_Chr7g0224271	28.967	397	261	11	100	485	287	673	1.37e-39	152
Msa1400310	MtrunA17_Chr8g0357601	29.136	405	263	12	92	485	161	552	1.88e-38	147
Msa1400310	MtrunA17_Chr3g0110971	26.161	409	256	10	99	486	205	588	2.04e-38	147
Msa1400310	MtrunA17_Chr8g0376381	28.328	293	203	4	199	486	233	523	9.34e-37	142
Msa1400310	MtrunA17_Chr7g0238891	28.351	388	254	11	108	485	367	740	1.91e-34	137

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1400310	AT3G03450.1	27.068	399	259	8	99	486	169	546	3.62e-43	160
Msa1400310	AT1G66350.1	25.907	386	263	7	102	486	144	507	1.75e-42	158
Msa1400310	AT2G01570.1	25.954	393	266	8	99	486	210	582	3.45e-40	153
Msa1400310	AT5G17490.1	27.806	392	249	10	104	486	151	517	1.23e-39	150
Msa1400310	AT1G14920.1	26.768	396	259	9	99	486	158	530	3.28e-39	149
Msa1400310	AT3G46600.2	26.750	400	243	14	105	485	3	371	5.97e-35	134
Msa1400310	AT2G29065.1	26.703	367	239	9	136	484	273	627	7.08e-35	138
Msa1400310	AT5G59450.1	27.250	400	246	14	108	485	222	598	1.09e-34	137
Msa1400310	AT3G46600.3	26.799	403	245	14	102	485	176	547	1.34e-34	136
Msa1400310	AT3G46600.1	26.799	403	245	14	102	485	208	579	2.40e-34	136

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Find 68 sgRNAs with CRISPR-Local

Find 158 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAATGAGAGAAACAAGGAGA+CGG	0.612044	tig0022428:-97662	Msa1400310:CDS
AACAAACCTATTCGCAAACA+AGG	0.532041	tig0022428:+97111	None:intergenic
AAGATGAAGAAAGGAAGGTT+AGG	0.502253	tig0022428:-96964	Msa1400310:CDS
AAGCCATGATATCAATTCCA+CGG	0.616214	tig0022428:-98158	Msa1400310:CDS
AAGCCGTGGAATTGATATCA+TGG	0.533713	tig0022428:+98155	None:intergenic
AAGGCTAAGTTAGAAGAAAT+TGG	0.303167	tig0022428:-97388	Msa1400310:CDS
AATAATCTTTACATTCCTTA+TGG	0.249550	tig0022428:-97940	Msa1400310:CDS
AATCAAGTAATGCAATTTGC+TGG	0.354696	tig0022428:-97574	Msa1400310:CDS
AATGGTAAGTGTTTAATAAT+TGG	0.167586	tig0022428:-96803	Msa1400310:CDS
AATTGCATTACTTGATTGAA+AGG	0.446453	tig0022428:+97580	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
GAAGATATAATAACACTTGC+AGG	-	tig0022428:96949-96968	Msa1400310:CDS	30.0%
TTTCATCTCATGAATGAAAG+TGG	-	tig0022428:97159-97178	Msa1400310:CDS	30.0%
TAGATCAAATGAGAGAAACA+AGG	-	tig0022428:97281-97300	Msa1400310:CDS	30.0%
GACGGAATTGATTGATAAAA+TGG	-	tig0022428:97305-97324	Msa1400310:CDS	30.0%
AATCAAGTAATGCAATTTGC+TGG	-	tig0022428:97375-97394	Msa1400310:CDS	30.0%
AAGGCTAAGTTAGAAGAAAT+TGG	-	tig0022428:97561-97580	Msa1400310:CDS	30.0%
TTTATTCTCCATGTTCACTT+AGG	-	tig0022428:97709-97728	Msa1400310:CDS	30.0%
ACTATGGTATCAAATTCTGA+TGG	-	tig0022428:97732-97751	Msa1400310:CDS	30.0%
TTATGAGTGTAGAGTGATAA+TGG	-	tig0022428:97920-97939	Msa1400310:CDS	30.0%
ATGGAAGCAATATTATCTGA+AGG	-	tig0022428:97939-97958	Msa1400310:CDS	30.0%

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Chromosome	Type	Strat	End	Strand	Name
tig0022428	gene	96754	98217	96754	ID=Msa1400310;Name=Msa1400310
tig0022428	mRNA	96754	98217	96754	ID=Msa1400310-mRNA-1;Parent=Msa1400310;Name=Msa1400310-mRNA-1;_AED=0.01;_eAED=0.01;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|487
tig0022428	exon	96754	98217	96754	ID=Msa1400310-mRNA-1:exon:17669;Parent=Msa1400310-mRNA-1
tig0022428	CDS	96754	98217	96754	ID=Msa1400310-mRNA-1:cds;Parent=Msa1400310-mRNA-1

Gene Sequence

>Msa1400310

ATGGAGAAGTTACTACAAGATCTAGAGCAAGAAAATCAAGCCATGATATCAATTCCACGGCTTAAGCAGGTGCAAATGCCACCACCATCTTCTTTAGCATCACTTGAGCTATTGAAAAATTATGGAAGCAAGTTCAATAAGCAAAAAAGCAAAAGCAATGTTGAAACTTGTGTAAGTCCACAAAAGTTGTCAACTGAAGATATAATAACACTTGCAGGAACGAAGTATATCCAAAACTCATCACTGTGGCATGATAATAATCTTTACATTCCTTATGGAACTGTTCTTGGAAGCTTGTTGTCGGACGAAGAAAATAAACAAGTTGAACTAGCTCAGTTTGTTCTAGCAGCAGCTGAGAGAGTAGGTTGTCAACAATTTGAACGTGCAAACATGTTACTTTCACATTTTCATCTCATGAATGAAAGTGGAAATATTGGAAGCACAGTTCAGAGACTTCTTTTCCATTTCTCTCAAGCACTTCAAGAGAGAATCAACAGAGAAACCGGACGAGGAAAGATGAAACTGGATAGATCAAATGAGAGAAACAAGGAGACGGAATTGATTGATAAAATGGAATTAGATACTAACATAGTAGTTAAATGTCATCAAAAAATTCCTTTCAATCAAGTAATGCAATTTGCTGGTGTACAAGCTATAGTGGAACATGTTGCATCACAAACAAAAATCCATTTGATTCATCTTAATATTGGATGTGGAGTGATGAGTACATGTTTCATGCAAGCATTAGTAGACCGCAAGGAAAAGCCGGTTGAAATTCTGAAGATCACAGCTATTGGATTTTCTAACAAGGCTAAGTTAGAAGAAATTGGCAAAAGTTTAATGAGTTTTGCTGAATCCTTGAACATACCCTTTTTATATAACATAGTTTTTGTTGAAGATGCTATGGAGATTAAGTTAGATCAATTTGAAATTGAATATGATGAAGCTGTTGCTATTTATTCTCCATGTTCACTTAGGACTATGGTATCAAATTCTGATGGCTTGGAGAATTTGATGAAGGTGATAAGAAAAATGAAACCGTTTATTATGATAGTACTTGAACTTGAAGCAAACCATAATTCACCCTTGTTTGCGAATAGGTTTGTTGAAGCATTGTTCTTTTACTCAGCATTTTTTGACTGTGTTGATACTTGTATAAAAGAGGATTATGAGTGTAGAGTGATAATGGAAGCAATATTATCTGAAGGGATAAGAAACATTGTTGGTTTGGAAGATGAAGAAAGGAAGGTTAGGAATGTGAAGATTGATGTTTGGAGAAGGTTCTTTGCAAGGTATAGAATGGTAGAAACTGGATTCAGTGAGTCTTCAATTTATCAAGCTAATTTAGTTACTAAAAAGTTTGACTGTGGGAAGTTTTGTACTATAGACAAAAATGGTAAGTGTTTAATAATTGGATGGAAGGGGACTCCAATTCATTCAGTCTCAGCTTGGAAGTTTCTTTGA

Protein sequence

>Msa1400310

MEKLLQDLEQENQAMISIPRLKQVQMPPPSSLASLELLKNYGSKFNKQKSKSNVETCVSPQKLSTEDIITLAGTKYIQNSSLWHDNNLYIPYGTVLGSLLSDEENKQVELAQFVLAAAERVGCQQFERANMLLSHFHLMNESGNIGSTVQRLLFHFSQALQERINRETGRGKMKLDRSNERNKETELIDKMELDTNIVVKCHQKIPFNQVMQFAGVQAIVEHVASQTKIHLIHLNIGCGVMSTCFMQALVDRKEKPVEILKITAIGFSNKAKLEEIGKSLMSFAESLNIPFLYNIVFVEDAMEIKLDQFEIEYDEAVAIYSPCSLRTMVSNSDGLENLMKVIRKMKPFIMIVLELEANHNSPLFANRFVEALFFYSAFFDCVDTCIKEDYECRVIMEAILSEGIRNIVGLEDEERKVRNVKIDVWRRFFARYRMVETGFSESSIYQANLVTKKFDCGKFCTIDKNGKCLIIGWKGTPIHSVSAWKFL*