AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1401850

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1401850 MtrunA17_Chr5g0447191 95.699 186 8 0 122 307 3 188 7.11e-130 367
Msa1401850 MtrunA17_Chr5g0447111 42.857 329 149 10 6 306 8 325 3.62e-79 243
Msa1401850 MtrunA17_Chr3g0103031 45.104 337 141 10 1 306 1 324 1.10e-78 242
Msa1401850 MtrunA17_Chr1g0159831 34.731 334 172 10 7 306 5 326 1.69e-55 182
Msa1401850 MtrunA17_Chr3g0124921 36.556 331 166 11 7 306 4 321 5.52e-55 181
Msa1401850 MtrunA17_Chr4g0029841 34.862 218 126 3 88 305 76 277 1.14e-31 119
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1401850 AT1G55760.1 42.515 334 153 9 1 306 1 323 1.45e-75 234
Msa1401850 AT1G21780.2 37.690 329 163 9 7 306 5 320 1.52e-60 196
Msa1401850 AT1G21780.1 37.690 329 163 9 7 306 5 320 1.52e-60 196

Find 50 sgRNAs with CRISPR-Local

Find 172 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCTCAATATCTAGTGGAATT+TGG 0.233852 tig0022765:+62018 Msa1401850:CDS
TTGGTAAAATACATGAAATC+TGG 0.300649 tig0022765:+62037 Msa1401850:CDS
GGTTTGCAGTCTTGAGATCT+AGG 0.312817 tig0022765:-61388 None:intergenic
GTGAAACTGATGCATTTAAC+TGG 0.343547 tig0022765:+61312 Msa1401850:CDS
TCAAGATATCTAAATGGAAT+TGG 0.352470 tig0022765:+60444 Msa1401850:CDS
AAGCTTTCAAGATATCTAAA+TGG 0.359355 tig0022765:+60438 Msa1401850:CDS
TATGTTTGCACAAGATTCTA+AGG 0.372257 tig0022765:+61732 Msa1401850:CDS
CCCACGGATTCCACTTTGAA+TGG 0.379615 tig0022765:-60344 None:intergenic
GCATATAATTTGATAGTAAC+CGG 0.381945 tig0022765:-60725 None:intergenic
TTTAGATATCTTGAAAGCTT+CGG 0.402228 tig0022765:-60435 None:intergenic
ATGAATGCTTGACAAACTTC+AGG 0.409567 tig0022765:-61793 None:intergenic
GAATCATAGTTTCGAGCTAT+TGG 0.410266 tig0022765:+61855 Msa1401850:CDS
ATCTAGCTGTTCTTGCTGCC+CGG 0.416939 tig0022765:+61692 Msa1401850:CDS
CGGAATTTGAAAGATGCAAT+TGG 0.422404 tig0022765:-60770 None:intergenic
CCGTGGGTTACGTCGCTCAA+TGG 0.441645 tig0022765:+60360 Msa1401850:CDS
GATAGTAACCGGACTGGAAA+GGG 0.453381 tig0022765:-60714 None:intergenic
TGCAATTGGTGGATTATTTG+TGG 0.455591 tig0022765:-60756 None:intergenic
GAGGGATTCCCTTTCCAGTC+CGG 0.470003 tig0022765:+60706 Msa1401850:CDS
TGGTAAAATACATGAAATCT+GGG 0.484453 tig0022765:+62038 Msa1401850:CDS
TTGGTTGCTGCTGAGAAATA+CGG 0.484832 tig0022765:+61874 Msa1401850:CDS
TACAATCAAGCTCGTATCAT+CGG 0.487102 tig0022765:+61651 Msa1401850:CDS
GTTAAATGCATCAGTTTCAC+TGG 0.496821 tig0022765:-61309 None:intergenic
AATTTGAAAGATGCAATTGG+TGG 0.524532 tig0022765:-60767 None:intergenic
ACACAGGATGTTGTAACATC+AGG 0.530431 tig0022765:-61353 None:intergenic
TTAACTAAGGAAGAAATTCA+CGG 0.535488 tig0022765:-62116 None:intergenic
TTCTCTTAACATTCGAACAA+GGG 0.536373 tig0022765:-61611 None:intergenic
TATATTCAAACATAGGTGAC+CGG 0.536701 tig0022765:-61711 None:intergenic
TGATAGTAACCGGACTGGAA+AGG 0.543693 tig0022765:-60715 None:intergenic
AGCGCAACTAATCTTCAAGT+AGG 0.555426 tig0022765:-61998 None:intergenic
TTTCTCTTAACATTCGAACA+AGG 0.556453 tig0022765:-61612 None:intergenic
TCTTGAAAGCTTCGGATGTG+TGG 0.556978 tig0022765:-60427 None:intergenic
TCCATTCAAAGTGGAATCCG+TGG 0.560598 tig0022765:+60343 Msa1401850:CDS
GCAAACATATATTCAAACAT+AGG 0.563128 tig0022765:-61718 None:intergenic
ATGGAGAAACTAACTCAACT+AGG 0.576117 tig0022765:+61575 Msa1401850:CDS
TAATTTGATAGTAACCGGAC+TGG 0.592751 tig0022765:-60720 None:intergenic
GCGCAACTAATCTTCAAGTA+GGG 0.602481 tig0022765:-61997 None:intergenic
TAGGAATTCAACTTCAACAC+AGG 0.603549 tig0022765:-61369 None:intergenic
CCATTGAGCGACGTAACCCA+CGG 0.604930 tig0022765:-60360 None:intergenic
GGATGTGTGGATAAAACTGT+GGG 0.608090 tig0022765:-60414 None:intergenic
TAGTTGCGCTCAATATCTAG+TGG 0.608180 tig0022765:+62011 Msa1401850:CDS
CCATTCAAAGTGGAATCCGT+GGG 0.610952 tig0022765:+60344 Msa1401850:CDS
ATTGATAGAAATATGCAGCA+TGG 0.618155 tig0022765:-61972 None:intergenic
CGGCGTTAGTGACTTGAGAG+AGG 0.632778 tig0022765:+61894 Msa1401850:CDS
AAAGGCACATATCATTCTCG+AGG 0.636899 tig0022765:+60687 Msa1401850:intron
CGGATGTGTGGATAAAACTG+TGG 0.637469 tig0022765:-60415 None:intergenic
TGAAACTGATGCATTTAACT+GGG 0.643694 tig0022765:+61313 Msa1401850:CDS
ATATTCAAACATAGGTGACC+GGG 0.654376 tig0022765:-61710 None:intergenic
AAGGCACATATCATTCTCGA+GGG 0.667706 tig0022765:+60688 Msa1401850:intron
GCTTGATTGTAGCGTCTGTG+TGG 0.704769 tig0022765:-61639 None:intergenic
GATGTGTGGATAAAACTGTG+GGG 0.837656 tig0022765:-60413 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTATTTCAATTATATCATTA+AGG + tig0022765:60905-60924 Msa1401850:intron 10.0%
!!! ATAATTTTAGTATTGATATT+AGG + tig0022765:61496-61515 Msa1401850:intron 10.0%
!! TTATTTCAATTATATCATTA+AGG + tig0022765:60905-60924 Msa1401850:intron 10.0%
!!! ATAATTTTAGTATTGATATT+AGG + tig0022765:61496-61515 Msa1401850:intron 10.0%
!!! TGTTTAAATTTTTTGAATGA+AGG + tig0022765:61552-61571 Msa1401850:intron 15.0%
!!! TGTTTAAATTTTTTGAATGA+AGG + tig0022765:61552-61571 Msa1401850:intron 15.0%
!! CTCTAATCATAAAAATCATA+TGG + tig0022765:60543-60562 Msa1401850:intron 20.0%
!! TTACTTATAGAATTATGAGT+TGG + tig0022765:60873-60892 Msa1401850:intron 20.0%
!! ATTATAGGTATATATGTTTG+TGG - tig0022765:61049-61068 None:intergenic 20.0%
!! ATGCTTATATCTATATGAAT+AGG + tig0022765:61109-61128 Msa1401850:intron 20.0%
!! TGCTTATATCTATATGAATA+GGG + tig0022765:61110-61129 Msa1401850:intron 20.0%
!!! TTTCATAGACAAAGATTTTT+AGG - tig0022765:61172-61191 None:intergenic 20.0%
!! ATTGAATAAAACAAGACATA+TGG + tig0022765:61209-61228 Msa1401850:intron 20.0%
!!! TAAATTTTTTGAATGAAGGA+TGG + tig0022765:61556-61575 Msa1401850:intron 20.0%
!! CTCTAATCATAAAAATCATA+TGG + tig0022765:60543-60562 Msa1401850:intron 20.0%
!! TTACTTATAGAATTATGAGT+TGG + tig0022765:60873-60892 Msa1401850:intron 20.0%
!! ATTATAGGTATATATGTTTG+TGG - tig0022765:61049-61068 None:intergenic 20.0%
!! ATGCTTATATCTATATGAAT+AGG + tig0022765:61109-61128 Msa1401850:intron 20.0%
!! TGCTTATATCTATATGAATA+GGG + tig0022765:61110-61129 Msa1401850:intron 20.0%
!!! TTTCATAGACAAAGATTTTT+AGG - tig0022765:61172-61191 None:intergenic 20.0%
!! ATTGAATAAAACAAGACATA+TGG + tig0022765:61209-61228 Msa1401850:intron 20.0%
!!! TAAATTTTTTGAATGAAGGA+TGG + tig0022765:61556-61575 Msa1401850:intron 20.0%
! TTTAGATATCTTGAAAGCTT+CGG - tig0022765:60438-60457 None:intergenic 25.0%
! AAGCTTTCAAGATATCTAAA+TGG + tig0022765:60438-60457 Msa1401850:CDS 25.0%
! TCAAGATATCTAAATGGAAT+TGG + tig0022765:60444-60463 Msa1401850:CDS 25.0%
! ATCATAAAAATCATATGGTC+TGG + tig0022765:60548-60567 Msa1401850:intron 25.0%
!! GCATATAATTTGATAGTAAC+CGG - tig0022765:60728-60747 None:intergenic 25.0%
! CTTTCAGAATGTAACAAATT+AGG - tig0022765:61026-61045 None:intergenic 25.0%
!!! GTCTATGAAAATGTTGTTTT+TGG + tig0022765:61182-61201 Msa1401850:intron 25.0%
! AACAAGACATATGGTTAAAT+AGG + tig0022765:61218-61237 Msa1401850:intron 25.0%
! GCAAACATATATTCAAACAT+AGG - tig0022765:61721-61740 None:intergenic 25.0%
! ACAGAAAAAATATACTTGAG+AGG + tig0022765:61947-61966 Msa1401850:CDS 25.0%
! TTGGTAAAATACATGAAATC+TGG + tig0022765:62037-62056 Msa1401850:CDS 25.0%
! TGGTAAAATACATGAAATCT+GGG + tig0022765:62038-62057 Msa1401850:CDS 25.0%
! TTTAGATATCTTGAAAGCTT+CGG - tig0022765:60438-60457 None:intergenic 25.0%
! AAGCTTTCAAGATATCTAAA+TGG + tig0022765:60438-60457 Msa1401850:CDS 25.0%
! TCAAGATATCTAAATGGAAT+TGG + tig0022765:60444-60463 Msa1401850:CDS 25.0%
! ATCATAAAAATCATATGGTC+TGG + tig0022765:60548-60567 Msa1401850:intron 25.0%
!! GCATATAATTTGATAGTAAC+CGG - tig0022765:60728-60747 None:intergenic 25.0%
! CTTTCAGAATGTAACAAATT+AGG - tig0022765:61026-61045 None:intergenic 25.0%
!!! GTCTATGAAAATGTTGTTTT+TGG + tig0022765:61182-61201 Msa1401850:intron 25.0%
! AACAAGACATATGGTTAAAT+AGG + tig0022765:61218-61237 Msa1401850:intron 25.0%
! GCAAACATATATTCAAACAT+AGG - tig0022765:61721-61740 None:intergenic 25.0%
! ACAGAAAAAATATACTTGAG+AGG + tig0022765:61947-61966 Msa1401850:CDS 25.0%
! TTGGTAAAATACATGAAATC+TGG + tig0022765:62037-62056 Msa1401850:CDS 25.0%
! TGGTAAAATACATGAAATCT+GGG + tig0022765:62038-62057 Msa1401850:CDS 25.0%
CCAACGTTATGATGAATAAT+CGG - tig0022765:60577-60596 None:intergenic 30.0%
!!! TTTTTTACTTCCCAACACAA+AGG + tig0022765:60669-60688 Msa1401850:intron 30.0%
AATTTGAAAGATGCAATTGG+TGG - tig0022765:60770-60789 None:intergenic 30.0%
TGAAACTGATGCATTTAACT+GGG + tig0022765:61313-61332 Msa1401850:CDS 30.0%
TTCTCTTAACATTCGAACAA+GGG - tig0022765:61614-61633 None:intergenic 30.0%
TTTCTCTTAACATTCGAACA+AGG - tig0022765:61615-61634 None:intergenic 30.0%
TATATTCAAACATAGGTGAC+CGG - tig0022765:61714-61733 None:intergenic 30.0%
TATGTTTGCACAAGATTCTA+AGG + tig0022765:61732-61751 Msa1401850:CDS 30.0%
ATTGATAGAAATATGCAGCA+TGG - tig0022765:61975-61994 None:intergenic 30.0%
! TTTTCTTGCCTTCCTAAAAT+TGG + tig0022765:62065-62084 Msa1401850:CDS 30.0%
CCAACGTTATGATGAATAAT+CGG - tig0022765:60577-60596 None:intergenic 30.0%
!!! TTTTTTACTTCCCAACACAA+AGG + tig0022765:60669-60688 Msa1401850:intron 30.0%
AATTTGAAAGATGCAATTGG+TGG - tig0022765:60770-60789 None:intergenic 30.0%
TGAAACTGATGCATTTAACT+GGG + tig0022765:61313-61332 Msa1401850:CDS 30.0%
TTCTCTTAACATTCGAACAA+GGG - tig0022765:61614-61633 None:intergenic 30.0%
TTTCTCTTAACATTCGAACA+AGG - tig0022765:61615-61634 None:intergenic 30.0%
TATATTCAAACATAGGTGAC+CGG - tig0022765:61714-61733 None:intergenic 30.0%
TATGTTTGCACAAGATTCTA+AGG + tig0022765:61732-61751 Msa1401850:CDS 30.0%
ATTGATAGAAATATGCAGCA+TGG - tig0022765:61975-61994 None:intergenic 30.0%
! TTTTCTTGCCTTCCTAAAAT+TGG + tig0022765:62065-62084 Msa1401850:CDS 30.0%
! GTTTTACTCTCCATTCAAAG+TGG + tig0022765:60334-60353 Msa1401850:CDS 35.0%
CCGATTATTCATCATAACGT+TGG + tig0022765:60574-60593 Msa1401850:intron 35.0%
ATGATATGTGCCTTTGTGTT+GGG - tig0022765:60682-60701 None:intergenic 35.0%
AATGATATGTGCCTTTGTGT+TGG - tig0022765:60683-60702 None:intergenic 35.0%
! TAATTTGATAGTAACCGGAC+TGG - tig0022765:60723-60742 None:intergenic 35.0%
!! TGCAATTGGTGGATTATTTG+TGG - tig0022765:60759-60778 None:intergenic 35.0%
CGGAATTTGAAAGATGCAAT+TGG - tig0022765:60773-60792 None:intergenic 35.0%
TTCCGTATTCTTGACTCTAT+CGG + tig0022765:60788-60807 Msa1401850:intron 35.0%
TTCCGATAGAGTCAAGAATA+CGG - tig0022765:60793-60812 None:intergenic 35.0%
CTTGACTCTATCGGAAAAAA+TGG + tig0022765:60797-60816 Msa1401850:intron 35.0%
TATGAATAGGGACTATGAGT+TGG + tig0022765:61122-61141 Msa1401850:intron 35.0%
! CTTTTTCTTCTGCATCATTG+TGG - tig0022765:61277-61296 None:intergenic 35.0%
GTTAAATGCATCAGTTTCAC+TGG - tig0022765:61312-61331 None:intergenic 35.0%
GTGAAACTGATGCATTTAAC+TGG + tig0022765:61312-61331 Msa1401850:CDS 35.0%
TAGGAATTCAACTTCAACAC+AGG - tig0022765:61372-61391 None:intergenic 35.0%
GATTGAATGCACGTACATTT+GGG - tig0022765:61412-61431 None:intergenic 35.0%
TGATTGAATGCACGTACATT+TGG - tig0022765:61413-61432 None:intergenic 35.0%
ATGGAGAAACTAACTCAACT+AGG + tig0022765:61575-61594 Msa1401850:CDS 35.0%
TACAATCAAGCTCGTATCAT+CGG + tig0022765:61651-61670 Msa1401850:CDS 35.0%
ATATTCAAACATAGGTGACC+GGG - tig0022765:61713-61732 None:intergenic 35.0%
ATGAATGCTTGACAAACTTC+AGG - tig0022765:61796-61815 None:intergenic 35.0%
GAATCATAGTTTCGAGCTAT+TGG + tig0022765:61855-61874 Msa1401850:CDS 35.0%
GCTCAATATCTAGTGGAATT+TGG + tig0022765:62018-62037 Msa1401850:CDS 35.0%
!! ATCATCTGCCAATTTTAGGA+AGG - tig0022765:62076-62095 None:intergenic 35.0%
! CATCATCATCTGCCAATTTT+AGG - tig0022765:62080-62099 None:intergenic 35.0%
! GTTTTACTCTCCATTCAAAG+TGG + tig0022765:60334-60353 Msa1401850:CDS 35.0%
CCGATTATTCATCATAACGT+TGG + tig0022765:60574-60593 Msa1401850:intron 35.0%
ATGATATGTGCCTTTGTGTT+GGG - tig0022765:60682-60701 None:intergenic 35.0%
AATGATATGTGCCTTTGTGT+TGG - tig0022765:60683-60702 None:intergenic 35.0%
! TAATTTGATAGTAACCGGAC+TGG - tig0022765:60723-60742 None:intergenic 35.0%
!! TGCAATTGGTGGATTATTTG+TGG - tig0022765:60759-60778 None:intergenic 35.0%
CGGAATTTGAAAGATGCAAT+TGG - tig0022765:60773-60792 None:intergenic 35.0%
TTCCGTATTCTTGACTCTAT+CGG + tig0022765:60788-60807 Msa1401850:intron 35.0%
TTCCGATAGAGTCAAGAATA+CGG - tig0022765:60793-60812 None:intergenic 35.0%
CTTGACTCTATCGGAAAAAA+TGG + tig0022765:60797-60816 Msa1401850:intron 35.0%
TATGAATAGGGACTATGAGT+TGG + tig0022765:61122-61141 Msa1401850:intron 35.0%
! CTTTTTCTTCTGCATCATTG+TGG - tig0022765:61277-61296 None:intergenic 35.0%
GTTAAATGCATCAGTTTCAC+TGG - tig0022765:61312-61331 None:intergenic 35.0%
GTGAAACTGATGCATTTAAC+TGG + tig0022765:61312-61331 Msa1401850:CDS 35.0%
TAGGAATTCAACTTCAACAC+AGG - tig0022765:61372-61391 None:intergenic 35.0%
GATTGAATGCACGTACATTT+GGG - tig0022765:61412-61431 None:intergenic 35.0%
TGATTGAATGCACGTACATT+TGG - tig0022765:61413-61432 None:intergenic 35.0%
ATGGAGAAACTAACTCAACT+AGG + tig0022765:61575-61594 Msa1401850:CDS 35.0%
TACAATCAAGCTCGTATCAT+CGG + tig0022765:61651-61670 Msa1401850:CDS 35.0%
ATATTCAAACATAGGTGACC+GGG - tig0022765:61713-61732 None:intergenic 35.0%
ATGAATGCTTGACAAACTTC+AGG - tig0022765:61796-61815 None:intergenic 35.0%
GAATCATAGTTTCGAGCTAT+TGG + tig0022765:61855-61874 Msa1401850:CDS 35.0%
GCTCAATATCTAGTGGAATT+TGG + tig0022765:62018-62037 Msa1401850:CDS 35.0%
!! ATCATCTGCCAATTTTAGGA+AGG - tig0022765:62076-62095 None:intergenic 35.0%
! CATCATCATCTGCCAATTTT+AGG - tig0022765:62080-62099 None:intergenic 35.0%
GATGTGTGGATAAAACTGTG+GGG - tig0022765:60416-60435 None:intergenic 40.0%
GGATGTGTGGATAAAACTGT+GGG - tig0022765:60417-60436 None:intergenic 40.0%
! AAAGGCACATATCATTCTCG+AGG + tig0022765:60687-60706 Msa1401850:intron 40.0%
AAGGCACATATCATTCTCGA+GGG + tig0022765:60688-60707 Msa1401850:intron 40.0%
TCTGCATCATTGTGGAAAAG+AGG - tig0022765:61269-61288 None:intergenic 40.0%
ACACAGGATGTTGTAACATC+AGG - tig0022765:61356-61375 None:intergenic 40.0%
TTGGTTGCTGCTGAGAAATA+CGG + tig0022765:61874-61893 Msa1401850:CDS 40.0%
GCGCAACTAATCTTCAAGTA+GGG - tig0022765:62000-62019 None:intergenic 40.0%
AGCGCAACTAATCTTCAAGT+AGG - tig0022765:62001-62020 None:intergenic 40.0%
TAGTTGCGCTCAATATCTAG+TGG + tig0022765:62011-62030 Msa1401850:CDS 40.0%
GATGTGTGGATAAAACTGTG+GGG - tig0022765:60416-60435 None:intergenic 40.0%
GGATGTGTGGATAAAACTGT+GGG - tig0022765:60417-60436 None:intergenic 40.0%
! AAAGGCACATATCATTCTCG+AGG + tig0022765:60687-60706 Msa1401850:intron 40.0%
AAGGCACATATCATTCTCGA+GGG + tig0022765:60688-60707 Msa1401850:intron 40.0%
TCTGCATCATTGTGGAAAAG+AGG - tig0022765:61269-61288 None:intergenic 40.0%
ACACAGGATGTTGTAACATC+AGG - tig0022765:61356-61375 None:intergenic 40.0%
TTGGTTGCTGCTGAGAAATA+CGG + tig0022765:61874-61893 Msa1401850:CDS 40.0%
GCGCAACTAATCTTCAAGTA+GGG - tig0022765:62000-62019 None:intergenic 40.0%
AGCGCAACTAATCTTCAAGT+AGG - tig0022765:62001-62020 None:intergenic 40.0%
TAGTTGCGCTCAATATCTAG+TGG + tig0022765:62011-62030 Msa1401850:CDS 40.0%
TCCATTCAAAGTGGAATCCG+TGG + tig0022765:60343-60362 Msa1401850:CDS 45.0%
CCATTCAAAGTGGAATCCGT+GGG + tig0022765:60344-60363 Msa1401850:CDS 45.0%
CGGATGTGTGGATAAAACTG+TGG - tig0022765:60418-60437 None:intergenic 45.0%
TCTTGAAAGCTTCGGATGTG+TGG - tig0022765:60430-60449 None:intergenic 45.0%
! GATAGTAACCGGACTGGAAA+GGG - tig0022765:60717-60736 None:intergenic 45.0%
! TGATAGTAACCGGACTGGAA+AGG - tig0022765:60718-60737 None:intergenic 45.0%
TGGAATAACCTTCGCAGAGA+CGG + tig0022765:60817-60836 Msa1401850:intron 45.0%
!!! TTTTTTACCCGTCTCTGCGA+AGG - tig0022765:60828-60847 None:intergenic 45.0%
GGTTTGCAGTCTTGAGATCT+AGG - tig0022765:61391-61410 None:intergenic 45.0%
TCCATTCAAAGTGGAATCCG+TGG + tig0022765:60343-60362 Msa1401850:CDS 45.0%
CCATTCAAAGTGGAATCCGT+GGG + tig0022765:60344-60363 Msa1401850:CDS 45.0%
CGGATGTGTGGATAAAACTG+TGG - tig0022765:60418-60437 None:intergenic 45.0%
TCTTGAAAGCTTCGGATGTG+TGG - tig0022765:60430-60449 None:intergenic 45.0%
! GATAGTAACCGGACTGGAAA+GGG - tig0022765:60717-60736 None:intergenic 45.0%
! TGATAGTAACCGGACTGGAA+AGG - tig0022765:60718-60737 None:intergenic 45.0%
TGGAATAACCTTCGCAGAGA+CGG + tig0022765:60817-60836 Msa1401850:intron 45.0%
!!! TTTTTTACCCGTCTCTGCGA+AGG - tig0022765:60828-60847 None:intergenic 45.0%
GGTTTGCAGTCTTGAGATCT+AGG - tig0022765:61391-61410 None:intergenic 45.0%
!!! TTTATTTTGATTAAAATTAT+AGG - tig0022765:61064-61083 None:intergenic 5.0%
!!! TTTATTTTGATTAAAATTAT+AGG - tig0022765:61064-61083 None:intergenic 5.0%
CCCACGGATTCCACTTTGAA+TGG - tig0022765:60347-60366 None:intergenic 50.0%
GGAATAACCTTCGCAGAGAC+GGG + tig0022765:60818-60837 Msa1401850:intron 50.0%
GCTTGATTGTAGCGTCTGTG+TGG - tig0022765:61642-61661 None:intergenic 50.0%
ATCTAGCTGTTCTTGCTGCC+CGG + tig0022765:61692-61711 Msa1401850:CDS 50.0%
CCCACGGATTCCACTTTGAA+TGG - tig0022765:60347-60366 None:intergenic 50.0%
GGAATAACCTTCGCAGAGAC+GGG + tig0022765:60818-60837 Msa1401850:intron 50.0%
GCTTGATTGTAGCGTCTGTG+TGG - tig0022765:61642-61661 None:intergenic 50.0%
ATCTAGCTGTTCTTGCTGCC+CGG + tig0022765:61692-61711 Msa1401850:CDS 50.0%
CCATTGAGCGACGTAACCCA+CGG - tig0022765:60363-60382 None:intergenic 55.0%
GAGGGATTCCCTTTCCAGTC+CGG + tig0022765:60706-60725 Msa1401850:CDS 55.0%
CGGCGTTAGTGACTTGAGAG+AGG + tig0022765:61894-61913 Msa1401850:CDS 55.0%
CCATTGAGCGACGTAACCCA+CGG - tig0022765:60363-60382 None:intergenic 55.0%
GAGGGATTCCCTTTCCAGTC+CGG + tig0022765:60706-60725 Msa1401850:CDS 55.0%
CGGCGTTAGTGACTTGAGAG+AGG + tig0022765:61894-61913 Msa1401850:CDS 55.0%
! CCGTGGGTTACGTCGCTCAA+TGG + tig0022765:60360-60379 Msa1401850:CDS 60.0%
! CCGTGGGTTACGTCGCTCAA+TGG + tig0022765:60360-60379 Msa1401850:CDS 60.0%
Chromosome Type Strat End Strand Name
tig0022765 gene 60332 62134 60332 ID=Msa1401850;Name=Msa1401850
tig0022765 mRNA 60332 62134 60332 ID=Msa1401850-mRNA-1;Parent=Msa1401850;Name=Msa1401850-mRNA-1;_AED=0.03;_eAED=0.03;_QI=0|0|0|1|0.33|0.75|4|0|307
tig0022765 exon 60332 60465 60332 ID=Msa1401850-mRNA-1:exon:18609;Parent=Msa1401850-mRNA-1
tig0022765 exon 60691 60791 60691 ID=Msa1401850-mRNA-1:exon:18610;Parent=Msa1401850-mRNA-1
tig0022765 exon 61288 61415 61288 ID=Msa1401850-mRNA-1:exon:18611;Parent=Msa1401850-mRNA-1
tig0022765 exon 61574 62134 61574 ID=Msa1401850-mRNA-1:exon:18612;Parent=Msa1401850-mRNA-1
tig0022765 CDS 60332 60465 60332 ID=Msa1401850-mRNA-1:cds;Parent=Msa1401850-mRNA-1
tig0022765 CDS 60691 60791 60691 ID=Msa1401850-mRNA-1:cds;Parent=Msa1401850-mRNA-1
tig0022765 CDS 61288 61415 61288 ID=Msa1401850-mRNA-1:cds;Parent=Msa1401850-mRNA-1
tig0022765 CDS 61574 62134 61574 ID=Msa1401850-mRNA-1:cds;Parent=Msa1401850-mRNA-1
Gene Sequence

>Msa1401850

ATGTTTTACTCTCCATTCAAAGTGGAATCCGTGGGTTACGTCGCTCAATGGAGACTCAGCTTTAAGAGCATGAATATGATTCCCCACAGTTTTATCCACACATCCGAAGCTTTCAAGATATCTAAATGGAATTGGCACATATCATTCTCGAGGGATTCCCTTTCCAGTCCGGTTACTATCAAATTATATGCAGAAGAGTCCACAAATAATCCACCAATTGCATCTTTCAAATTCCAAGAAAAAGACAAGCTTTTCACCAGTGAAACTGATGCATTTAACTGGGAGTTTGATGTTCGACTACCTGATGTTACAACATCCTGTGTTGAAGTTGAATTCCTAGATCTCAAGACTGCAAACCCAAATGATGGAGAAACTAACTCAACTAGGAAAAGATCAACAACCCTTGTTCGAATGTTAAGAGAAAGTATCCACACAGACGCTACAATCAAGCTCGTATCATCGGATAAAATCATAAGAGTTCATCTAGCTGTTCTTGCTGCCCGGTCACCTATGTTTGAATATATGTTTGCACAAGATTCTAAGGAGAATAACATGTCTGTTATTAACATCTCAGATATGTCACCTGAAGTTTGTCAAGCATTCATAGATTATCTATATGACAACATCGACGATGAAGAACTTGTGAATCATAGTTTCGAGCTATTGGTTGCTGCTGAGAAATACGGCGTTAGTGACTTGAGAGAGGAGTGTGAAAAGAATCTTCAAGAAGATATCAACAGAAAAAATATACTTGAGAGGCTCCATGCTGCATATTTCTATCAATTGCCCTACTTGAAGATTAGTTGCGCTCAATATCTAGTGGAATTTGGTAAAATACATGAAATCTGGGATGATTTTCTTGCCTTCCTAAAATTGGCAGATGATGATGTAATTTGCGAAATTTTCCGTGAATTTCTTCCTTAG
Protein sequence

>Msa1401850

MFYSPFKVESVGYVAQWRLSFKSMNMIPHSFIHTSEAFKISKWNWHISFSRDSLSSPVTIKLYAEESTNNPPIASFKFQEKDKLFTSETDAFNWEFDVRLPDVTTSCVEVEFLDLKTANPNDGETNSTRKRSTTLVRMLRESIHTDATIKLVSSDKIIRVHLAVLAARSPMFEYMFAQDSKENNMSVINISDMSPEVCQAFIDYLYDNIDDEELVNHSFELLVAAEKYGVSDLREECEKNLQEDINRKNILERLHAAYFYQLPYLKISCAQYLVEFGKIHEIWDDFLAFLKLADDDVICEIFREFLP*