AlfalfaGEDB | Msa1407280

M. sativa cultivar zhongmu-4 / Msa1407280

KEGG: -
GO: -
PFAM: PF07714.20

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1407280	ACJ85716.1	74.138	58	15	0	127	184	140	197	5.27e-19	95.1
Msa1407280	ACJ85716.1	74.138	58	15	0	51	108	140	197	5.27e-19	95.1

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1407280	B7FLT3	74.138	58	15	0	127	184	140	197	2.52e-19	95.1
Msa1407280	B7FLT3	74.138	58	15	0	51	108	140	197	2.52e-19	95.1

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1407280	MtrunA17_Chr3g0111431	61.972	71	27	0	38	108	258	328	1.23e-22	94.7
Msa1407280	MtrunA17_Chr3g0111431	61.972	71	27	0	114	184	258	328	1.23e-22	94.7

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Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
Msa1407280	AT5G37790.1	66.667	48	16	61	108	313	360	8.37e-16	75.5
Msa1407280	AT5G37790.1	66.667	48	16	137	184	313	360	8.37e-16	75.5
Msa1407280	AT1G66460.2	64.583	48	17	61	108	258	305	3.54e-15	73.2
Msa1407280	AT1G66460.2	64.583	48	17	137	184	258	305	3.54e-15	73.2
Msa1407280	AT1G66460.3	64.583	48	17	61	108	258	305	4.47e-15	73.2
Msa1407280	AT1G66460.3	64.583	48	17	137	184	258	305	4.47e-15	73.2
Msa1407280	AT1G66460.1	64.583	48	17	61	108	231	278	4.51e-15	73.2
Msa1407280	AT1G66460.1	64.583	48	17	137	184	231	278	4.51e-15	73.2

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Find 49 sgRNAs with CRISPR-Local

Find 197 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AATTGGGATTGCAGAAGCTA+TGG	0.512027	tig0023279:+64666	Msa1407280:CDS
AATTGGGATTGCAGAAGCTA+TGG	0.512027	tig0023279:+65014	Msa1407280:CDS
ACCTTGTACCTCACAGACCA+AGG	0.588300	tig0023279:-64640	None:intergenic
ACCTTGTACCTCACAGACCA+AGG	0.588300	tig0023279:-64988	None:intergenic
AGAAGAAAGAAGAACGCTAA+AGG	0.495359	tig0023279:-64392	None:intergenic
AGAAGAAAGAAGAACGCTAA+AGG	0.495359	tig0023279:-64740	None:intergenic
AGAAGAAAGAAGAACGCTAA+AGG	0.495359	tig0023279:-65088	None:intergenic
AGGCTCCGAAGATGCGATTT+AGG	0.263115	tig0023279:+64419	Msa1407280:CDS
AGGCTCCGAAGATGCGATTT+AGG	0.263115	tig0023279:+64767	Msa1407280:CDS
AGGCTCCGAAGATGCGATTT+AGG	0.263115	tig0023279:+65115	Msa1407280:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
ATAACCAAATGTTCCTTTGA+CGG	-	tig0023279:64492-64511	None:intergenic	30.0%
CAAAGGAACATTTGGTTATT+TGG	+	tig0023279:64493-64512	Msa1407280:intron	30.0%
ATAACCAAATGTTCCTTTGA+CGG	-	tig0023279:64492-64511	None:intergenic	30.0%
CAAAGGAACATTTGGTTATT+TGG	+	tig0023279:64493-64512	Msa1407280:intron	30.0%
ATAACCAAATGTTCCTTTGA+CGG	-	tig0023279:64492-64511	None:intergenic	30.0%
CAAAGGAACATTTGGTTATT+TGG	+	tig0023279:64493-64512	Msa1407280:intron	30.0%
ATAACCAAATGTTCCTTTGA+CGG	-	tig0023279:64492-64511	None:intergenic	30.0%
CAAAGGAACATTTGGTTATT+TGG	+	tig0023279:64493-64512	Msa1407280:intron	30.0%
CTCTATGAACACATGTCTTT+CGG	-	tig0023279:64363-64382	None:intergenic	35.0%
GAAGAAAGAAGAACGCTAAA+GGG	-	tig0023279:64394-64413	None:intergenic	35.0%

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Chromosome	Type	Strat	End	Strand	Name
tig0023279	gene	64337	65182	64337	ID=Msa1407280;Name=Msa1407280
tig0023279	mRNA	64337	65182	64337	ID=Msa1407280-mRNA-1;Parent=Msa1407280;Name=Msa1407280-mRNA-1;_AED=0.36;_eAED=0.53;_QI=0\|0\|0\|1\|1\|1\|3\|0\|201
tig0023279	exon	64337	64469	64337	ID=Msa1407280-mRNA-1:exon:25004;Parent=Msa1407280-mRNA-1
tig0023279	exon	64590	64817	64590	ID=Msa1407280-mRNA-1:exon:25005;Parent=Msa1407280-mRNA-1
tig0023279	exon	64938	65182	64938	ID=Msa1407280-mRNA-1:exon:25006;Parent=Msa1407280-mRNA-1
tig0023279	CDS	64337	64469	64337	ID=Msa1407280-mRNA-1:cds;Parent=Msa1407280-mRNA-1
tig0023279	CDS	64590	64817	64590	ID=Msa1407280-mRNA-1:cds;Parent=Msa1407280-mRNA-1
tig0023279	CDS	64938	65182	64938	ID=Msa1407280-mRNA-1:cds;Parent=Msa1407280-mRNA-1

Gene Sequence

>Msa1407280

ATGGCTTATCTGCATAATGGAAACCGAAAGACATGTGTTCATAGAGACATTAATCCCTTTAGCGTTCTTCTTTCTTCTAAGGAGGCTCCGAAGATGCGATTTAGGATTAGCTACATGGACTTGAACACCTTCGGAGGGTATGTTTTTGTGAGGAAGAACGGTAGGAATGGTAGTTCAACACTCCCTTGGTCTGTGAGGTACAAGGTTGCAATTGGGATTGCAGAAGCTATGGCTTATCTGCATAATGGAAACCGAAAGACATGTGTTCATAGAGACATTAATCCCTTTAGCGTTCTTCTTTCTTCTAAGGAGGCTCCGAAGATGCGATTTAGGATTAGCTACATGGACTTGAACACCTTCGGAGGGTATGTTTTTGTGAGGAAGAACGGTAGGAATGGTAGTTCAACACTCCCTTGGTCTGTGAGGTACAAGGTTGCAATTGGGATTGCAGAAGCTATGGCTTATCTGCATAATGGAAACCGAAAGACATGTGTTCATAGAGACATTAATCCCTTTAGCGTTCTTCTTTCTTCTAAGGAGGCTCCGAAGATGCGATTTAGGATTAGCTACATGGACTTGAACACCTTCGGTTCCTTTCCTTTGTAA

Protein sequence

>Msa1407280

MAYLHNGNRKTCVHRDINPFSVLLSSKEAPKMRFRISYMDLNTFGGYVFVRKNGRNGSSTLPWSVRYKVAIGIAEAMAYLHNGNRKTCVHRDINPFSVLLSSKEAPKMRFRISYMDLNTFGGYVFVRKNGRNGSSTLPWSVRYKVAIGIAEAMAYLHNGNRKTCVHRDINPFSVLLSSKEAPKMRFRISYMDLNTFGSFPL*