AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1408810


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1408810 MtrunA17_Chr8g0337271 77.551 196 32 1 1 184 1 196 9.10e-110 312
Msa1408810 MtrunA17_Chr8g0358731 31.000 200 89 7 1 186 1 165 3.99e-16 72.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 54 sgRNAs with CRISPR-Local

Find 122 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CATCCAAGGATGTGCTTATT+TGG 0.063042 tig0023416:+49551 None:intergenic
TTCAGTGTGTTATTCAAATT+TGG 0.105357 tig0023416:-49716 Msa1408810:CDS
CTGACTTAAACTTGAGTATA+TGG 0.197296 tig0023416:-49435 Msa1408810:CDS
CATGACACTGATTGTGAATT+TGG 0.306396 tig0023416:-49771 Msa1408810:CDS
TCAGTGTGTTATTCAAATTT+GGG 0.308325 tig0023416:-49715 Msa1408810:CDS
CAAATTCCCCATCTTACTTT+GGG 0.318196 tig0023416:+48946 None:intergenic
ATCCAAGGATGTGCTTATTT+GGG 0.326625 tig0023416:+49552 None:intergenic
TCAAATTCCCCATCTTACTT+TGG 0.345284 tig0023416:+48945 None:intergenic
TCTTGGACACCAATTGGAGC+TGG 0.391570 tig0023416:-49578 Msa1408810:CDS
AAAGTCTTCATCCTCATTAC+TGG 0.397078 tig0023416:+48970 None:intergenic
TTCATCAGCCACCATTCCCT+TGG 0.409411 tig0023416:+49027 None:intergenic
TGTCCAAGAAAACCCCGATC+TGG 0.424943 tig0023416:+49592 None:intergenic
TTTGGGCACCCAGCTCCAAT+TGG 0.428496 tig0023416:+49569 None:intergenic
GGGAATGGTGGCTGATGAAA+AGG 0.430935 tig0023416:-49023 Msa1408810:CDS
TGACTTAAACTTGAGTATAT+GGG 0.443514 tig0023416:-49434 Msa1408810:intron
CTTGGACACCAATTGGAGCT+GGG 0.456606 tig0023416:-49577 Msa1408810:CDS
TTGGCGAAGTAACCCAGATC+GGG 0.470404 tig0023416:-49605 Msa1408810:CDS
TGCCCAAATAAGCACATCCT+TGG 0.475102 tig0023416:-49554 Msa1408810:CDS
AGCAATTTCAATTTCATCCA+AGG 0.478006 tig0023416:+49537 None:intergenic
CATATACTCAAGTTTAAGTC+AGG 0.479889 tig0023416:+49436 None:intergenic
TCTCCTCTTTCACAAGTACA+TGG 0.497829 tig0023416:+49185 None:intergenic
TCCTTCTGTGAGAAGCGAAT+CGG 0.511515 tig0023416:+49633 None:intergenic
TACTGAACACATGGGTGACA+TGG 0.514848 tig0023416:-49237 Msa1408810:CDS
GCCGATTCGCTTCTCACAGA+AGG 0.517177 tig0023416:-49634 Msa1408810:CDS
ATTCCATGTACTTGTGAAAG+AGG 0.523001 tig0023416:-49188 Msa1408810:CDS
TGTACACAGTAATGCATCCA+AGG 0.526625 tig0023416:-49044 Msa1408810:intron
GTCCAAGAAAACCCCGATCT+GGG 0.526848 tig0023416:+49593 None:intergenic
CTGAAAGTCAGCATGCCTAC+TGG 0.528158 tig0023416:+49734 None:intergenic
TGTGCCGACATATGAGAAGA+AGG 0.531308 tig0023416:-49794 Msa1408810:CDS
GTGGCTGATGAAAAGGGAAA+AGG 0.537842 tig0023416:-49016 Msa1408810:CDS
CTTGGCGAAGTAACCCAGAT+CGG 0.540526 tig0023416:-49606 Msa1408810:CDS
CATGCCTTCTTCTCATATGT+CGG 0.541385 tig0023416:+49790 None:intergenic
GTCTTCATCCTCATTACTGG+TGG 0.547436 tig0023416:+48973 None:intergenic
GCAACAATTTGTCTTTGATG+TGG 0.547669 tig0023416:+49655 None:intergenic
TTCTCACAGAAGGAGAAGCT+TGG 0.549947 tig0023416:-49624 Msa1408810:CDS
GTGCAAGAAGGCATGGAAGA+AGG 0.556829 tig0023416:+49466 None:intergenic
CAGTGTGTTATTCAAATTTG+GGG 0.561228 tig0023416:-49714 Msa1408810:intron
CAGTAATGCATCCAAGGGAA+TGG 0.575632 tig0023416:-49038 Msa1408810:CDS
AATTTGAGTGCAAGAAGGCA+TGG 0.581783 tig0023416:+49459 None:intergenic
AAATGCATCAGAGAAATACA+AGG 0.593981 tig0023416:-49833 Msa1408810:CDS
TAATGCATCCAAGGGAATGG+TGG 0.611462 tig0023416:-49035 Msa1408810:CDS
ACAAGGACCCACCAGTAATG+AGG 0.613595 tig0023416:-48981 Msa1408810:CDS
CTTGTGAAAGAGGAGAATCA+AGG 0.617523 tig0023416:-49178 Msa1408810:intron
GGAATGGTGGCTGATGAAAA+GGG 0.618900 tig0023416:-49022 Msa1408810:CDS
CATGGGTGACATGGACATCA+CGG 0.621521 tig0023416:-49228 Msa1408810:CDS
TCAGGAATTTGAGTGCAAGA+AGG 0.621914 tig0023416:+49454 None:intergenic
TTAATAGAGTACTGAACACA+TGG 0.626411 tig0023416:-49246 Msa1408810:intron
GTACACAGTAATGCATCCAA+GGG 0.631300 tig0023416:-49043 Msa1408810:intron
TCTTCATCCTCATTACTGGT+GGG 0.637097 tig0023416:+48974 None:intergenic
AAAGGAATTCTTAATGAACA+AGG 0.657272 tig0023416:-48998 Msa1408810:CDS
TGGCGAAGTAACCCAGATCG+GGG 0.667644 tig0023416:-49604 Msa1408810:CDS
ATCACGGAACAAGACATGCA+AGG 0.684891 tig0023416:-49212 Msa1408810:CDS
AATGCATCAGAGAAATACAA+GGG 0.687321 tig0023416:-49832 Msa1408810:CDS
TAATAGAGTACTGAACACAT+GGG 0.740993 tig0023416:-49245 Msa1408810:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTGAAAAGAATGATAGATTT+TGG + tig0023416:49460-49479 None:intergenic 20.0%
!!! TTGAAAAGAATGATAGATTT+TGG + tig0023416:49460-49479 None:intergenic 20.0%
!! TTCAGTGTGTTATTCAAATT+TGG - tig0023416:49034-49053 Msa1408810:CDS 25.0%
!! TCAGTGTGTTATTCAAATTT+GGG - tig0023416:49035-49054 Msa1408810:CDS 25.0%
! TTAATTGCATAATTCTCATC+AGG + tig0023416:49070-49089 None:intergenic 25.0%
! TGACTTAAACTTGAGTATAT+GGG - tig0023416:49316-49335 Msa1408810:intron 25.0%
! AAAGGAATTCTTAATGAACA+AGG - tig0023416:49752-49771 Msa1408810:CDS 25.0%
!! TTCAGTGTGTTATTCAAATT+TGG - tig0023416:49034-49053 Msa1408810:CDS 25.0%
!! TCAGTGTGTTATTCAAATTT+GGG - tig0023416:49035-49054 Msa1408810:CDS 25.0%
! TTAATTGCATAATTCTCATC+AGG + tig0023416:49070-49089 None:intergenic 25.0%
! TGACTTAAACTTGAGTATAT+GGG - tig0023416:49316-49335 Msa1408810:intron 25.0%
! AAAGGAATTCTTAATGAACA+AGG - tig0023416:49752-49771 Msa1408810:CDS 25.0%
! CAGTGTGTTATTCAAATTTG+GGG - tig0023416:49036-49055 Msa1408810:CDS 30.0%
AGCAATTTCAATTTCATCCA+AGG + tig0023416:49216-49235 None:intergenic 30.0%
CATATACTCAAGTTTAAGTC+AGG + tig0023416:49317-49336 None:intergenic 30.0%
CTGACTTAAACTTGAGTATA+TGG - tig0023416:49315-49334 Msa1408810:intron 30.0%
ATGTGTTCAGTACTCTATTA+AGG + tig0023416:49505-49524 None:intergenic 30.0%
!! TTAATAGAGTACTGAACACA+TGG - tig0023416:49504-49523 Msa1408810:CDS 30.0%
!! TAATAGAGTACTGAACACAT+GGG - tig0023416:49505-49524 Msa1408810:CDS 30.0%
! CAGTGTGTTATTCAAATTTG+GGG - tig0023416:49036-49055 Msa1408810:CDS 30.0%
AGCAATTTCAATTTCATCCA+AGG + tig0023416:49216-49235 None:intergenic 30.0%
CATATACTCAAGTTTAAGTC+AGG + tig0023416:49317-49336 None:intergenic 30.0%
CTGACTTAAACTTGAGTATA+TGG - tig0023416:49315-49334 Msa1408810:intron 30.0%
ATGTGTTCAGTACTCTATTA+AGG + tig0023416:49505-49524 None:intergenic 30.0%
!! TTAATAGAGTACTGAACACA+TGG - tig0023416:49504-49523 Msa1408810:CDS 30.0%
!! TAATAGAGTACTGAACACAT+GGG - tig0023416:49505-49524 Msa1408810:CDS 30.0%
! CATGACACTGATTGTGAATT+TGG - tig0023416:48979-48998 Msa1408810:CDS 35.0%
GCAACAATTTGTCTTTGATG+TGG + tig0023416:49098-49117 None:intergenic 35.0%
ATCCAAGGATGTGCTTATTT+GGG + tig0023416:49201-49220 None:intergenic 35.0%
ATTCCATGTACTTGTGAAAG+AGG - tig0023416:49562-49581 Msa1408810:CDS 35.0%
AAAGTCTTCATCCTCATTAC+TGG + tig0023416:49783-49802 None:intergenic 35.0%
! AGACTTTTCCCAAAGTAAGA+TGG - tig0023416:49796-49815 Msa1408810:CDS 35.0%
! GACTTTTCCCAAAGTAAGAT+GGG - tig0023416:49797-49816 Msa1408810:CDS 35.0%
! ACTTTTCCCAAAGTAAGATG+GGG - tig0023416:49798-49817 Msa1408810:CDS 35.0%
CAAATTCCCCATCTTACTTT+GGG + tig0023416:49807-49826 None:intergenic 35.0%
TCAAATTCCCCATCTTACTT+TGG + tig0023416:49808-49827 None:intergenic 35.0%
! CATGACACTGATTGTGAATT+TGG - tig0023416:48979-48998 Msa1408810:CDS 35.0%
GCAACAATTTGTCTTTGATG+TGG + tig0023416:49098-49117 None:intergenic 35.0%
ATCCAAGGATGTGCTTATTT+GGG + tig0023416:49201-49220 None:intergenic 35.0%
ATTCCATGTACTTGTGAAAG+AGG - tig0023416:49562-49581 Msa1408810:CDS 35.0%
AAAGTCTTCATCCTCATTAC+TGG + tig0023416:49783-49802 None:intergenic 35.0%
! AGACTTTTCCCAAAGTAAGA+TGG - tig0023416:49796-49815 Msa1408810:CDS 35.0%
! GACTTTTCCCAAAGTAAGAT+GGG - tig0023416:49797-49816 Msa1408810:CDS 35.0%
! ACTTTTCCCAAAGTAAGATG+GGG - tig0023416:49798-49817 Msa1408810:CDS 35.0%
CAAATTCCCCATCTTACTTT+GGG + tig0023416:49807-49826 None:intergenic 35.0%
TCAAATTCCCCATCTTACTT+TGG + tig0023416:49808-49827 None:intergenic 35.0%
CATGCCTTCTTCTCATATGT+CGG + tig0023416:48963-48982 None:intergenic 40.0%
! GTCAATACTTTTCGACCAGT+AGG - tig0023416:49001-49020 Msa1408810:CDS 40.0%
CATCCAAGGATGTGCTTATT+TGG + tig0023416:49202-49221 None:intergenic 40.0%
AATTTGAGTGCAAGAAGGCA+TGG + tig0023416:49294-49313 None:intergenic 40.0%
TCAGGAATTTGAGTGCAAGA+AGG + tig0023416:49299-49318 None:intergenic 40.0%
TCTCCTCTTTCACAAGTACA+TGG + tig0023416:49568-49587 None:intergenic 40.0%
CTTGTGAAAGAGGAGAATCA+AGG - tig0023416:49572-49591 Msa1408810:CDS 40.0%
TGTACACAGTAATGCATCCA+AGG - tig0023416:49706-49725 Msa1408810:intron 40.0%
GTACACAGTAATGCATCCAA+GGG - tig0023416:49707-49726 Msa1408810:intron 40.0%
TCTTCATCCTCATTACTGGT+GGG + tig0023416:49779-49798 None:intergenic 40.0%
CATGCCTTCTTCTCATATGT+CGG + tig0023416:48963-48982 None:intergenic 40.0%
! GTCAATACTTTTCGACCAGT+AGG - tig0023416:49001-49020 Msa1408810:CDS 40.0%
CATCCAAGGATGTGCTTATT+TGG + tig0023416:49202-49221 None:intergenic 40.0%
AATTTGAGTGCAAGAAGGCA+TGG + tig0023416:49294-49313 None:intergenic 40.0%
TCAGGAATTTGAGTGCAAGA+AGG + tig0023416:49299-49318 None:intergenic 40.0%
TCTCCTCTTTCACAAGTACA+TGG + tig0023416:49568-49587 None:intergenic 40.0%
CTTGTGAAAGAGGAGAATCA+AGG - tig0023416:49572-49591 Msa1408810:CDS 40.0%
TGTACACAGTAATGCATCCA+AGG - tig0023416:49706-49725 Msa1408810:intron 40.0%
GTACACAGTAATGCATCCAA+GGG - tig0023416:49707-49726 Msa1408810:intron 40.0%
TCTTCATCCTCATTACTGGT+GGG + tig0023416:49779-49798 None:intergenic 40.0%
! TGTGCCGACATATGAGAAGA+AGG - tig0023416:48956-48975 Msa1408810:CDS 45.0%
TCCTTCTGTGAGAAGCGAAT+CGG + tig0023416:49120-49139 None:intergenic 45.0%
TTCTCACAGAAGGAGAAGCT+TGG - tig0023416:49126-49145 Msa1408810:intron 45.0%
! GGGTTTTCTTGGACACCAAT+TGG - tig0023416:49166-49185 Msa1408810:intron 45.0%
TGCCCAAATAAGCACATCCT+TGG - tig0023416:49196-49215 Msa1408810:CDS 45.0%
TACTGAACACATGGGTGACA+TGG - tig0023416:49513-49532 Msa1408810:CDS 45.0%
ATCACGGAACAAGACATGCA+AGG - tig0023416:49538-49557 Msa1408810:CDS 45.0%
CAGTAATGCATCCAAGGGAA+TGG - tig0023416:49712-49731 Msa1408810:intron 45.0%
TAATGCATCCAAGGGAATGG+TGG - tig0023416:49715-49734 Msa1408810:CDS 45.0%
GGAATGGTGGCTGATGAAAA+GGG - tig0023416:49728-49747 Msa1408810:CDS 45.0%
GTGGCTGATGAAAAGGGAAA+AGG - tig0023416:49734-49753 Msa1408810:CDS 45.0%
GTCTTCATCCTCATTACTGG+TGG + tig0023416:49780-49799 None:intergenic 45.0%
! TGTGCCGACATATGAGAAGA+AGG - tig0023416:48956-48975 Msa1408810:CDS 45.0%
TCCTTCTGTGAGAAGCGAAT+CGG + tig0023416:49120-49139 None:intergenic 45.0%
TTCTCACAGAAGGAGAAGCT+TGG - tig0023416:49126-49145 Msa1408810:intron 45.0%
! GGGTTTTCTTGGACACCAAT+TGG - tig0023416:49166-49185 Msa1408810:intron 45.0%
TGCCCAAATAAGCACATCCT+TGG - tig0023416:49196-49215 Msa1408810:CDS 45.0%
TACTGAACACATGGGTGACA+TGG - tig0023416:49513-49532 Msa1408810:CDS 45.0%
ATCACGGAACAAGACATGCA+AGG - tig0023416:49538-49557 Msa1408810:CDS 45.0%
CAGTAATGCATCCAAGGGAA+TGG - tig0023416:49712-49731 Msa1408810:intron 45.0%
TAATGCATCCAAGGGAATGG+TGG - tig0023416:49715-49734 Msa1408810:CDS 45.0%
GGAATGGTGGCTGATGAAAA+GGG - tig0023416:49728-49747 Msa1408810:CDS 45.0%
GTGGCTGATGAAAAGGGAAA+AGG - tig0023416:49734-49753 Msa1408810:CDS 45.0%
GTCTTCATCCTCATTACTGG+TGG + tig0023416:49780-49799 None:intergenic 45.0%
CTGAAAGTCAGCATGCCTAC+TGG + tig0023416:49019-49038 None:intergenic 50.0%
CTTGGCGAAGTAACCCAGAT+CGG - tig0023416:49144-49163 Msa1408810:intron 50.0%
TTGGCGAAGTAACCCAGATC+GGG - tig0023416:49145-49164 Msa1408810:intron 50.0%
! AACCCAGATCGGGGTTTTCT+TGG - tig0023416:49155-49174 Msa1408810:intron 50.0%
GTCCAAGAAAACCCCGATCT+GGG + tig0023416:49160-49179 None:intergenic 50.0%
TGTCCAAGAAAACCCCGATC+TGG + tig0023416:49161-49180 None:intergenic 50.0%
TCTTGGACACCAATTGGAGC+TGG - tig0023416:49172-49191 Msa1408810:intron 50.0%
CTTGGACACCAATTGGAGCT+GGG - tig0023416:49173-49192 Msa1408810:intron 50.0%
! GTGCAAGAAGGCATGGAAGA+AGG + tig0023416:49287-49306 None:intergenic 50.0%
CATGGGTGACATGGACATCA+CGG - tig0023416:49522-49541 Msa1408810:CDS 50.0%
TTCATCAGCCACCATTCCCT+TGG + tig0023416:49726-49745 None:intergenic 50.0%
GGGAATGGTGGCTGATGAAA+AGG - tig0023416:49727-49746 Msa1408810:CDS 50.0%
ACAAGGACCCACCAGTAATG+AGG - tig0023416:49769-49788 Msa1408810:CDS 50.0%
CTGAAAGTCAGCATGCCTAC+TGG + tig0023416:49019-49038 None:intergenic 50.0%
CTTGGCGAAGTAACCCAGAT+CGG - tig0023416:49144-49163 Msa1408810:intron 50.0%
TTGGCGAAGTAACCCAGATC+GGG - tig0023416:49145-49164 Msa1408810:intron 50.0%
! AACCCAGATCGGGGTTTTCT+TGG - tig0023416:49155-49174 Msa1408810:intron 50.0%
GTCCAAGAAAACCCCGATCT+GGG + tig0023416:49160-49179 None:intergenic 50.0%
TGTCCAAGAAAACCCCGATC+TGG + tig0023416:49161-49180 None:intergenic 50.0%
TCTTGGACACCAATTGGAGC+TGG - tig0023416:49172-49191 Msa1408810:intron 50.0%
CTTGGACACCAATTGGAGCT+GGG - tig0023416:49173-49192 Msa1408810:intron 50.0%
! GTGCAAGAAGGCATGGAAGA+AGG + tig0023416:49287-49306 None:intergenic 50.0%
CATGGGTGACATGGACATCA+CGG - tig0023416:49522-49541 Msa1408810:CDS 50.0%
TTCATCAGCCACCATTCCCT+TGG + tig0023416:49726-49745 None:intergenic 50.0%
GGGAATGGTGGCTGATGAAA+AGG - tig0023416:49727-49746 Msa1408810:CDS 50.0%
ACAAGGACCCACCAGTAATG+AGG - tig0023416:49769-49788 Msa1408810:CDS 50.0%
!! GCCGATTCGCTTCTCACAGA+AGG - tig0023416:49116-49135 Msa1408810:intron 55.0%
TGGCGAAGTAACCCAGATCG+GGG - tig0023416:49146-49165 Msa1408810:intron 55.0%
TTTGGGCACCCAGCTCCAAT+TGG + tig0023416:49184-49203 None:intergenic 55.0%
!! GCCGATTCGCTTCTCACAGA+AGG - tig0023416:49116-49135 Msa1408810:intron 55.0%
TGGCGAAGTAACCCAGATCG+GGG - tig0023416:49146-49165 Msa1408810:intron 55.0%
TTTGGGCACCCAGCTCCAAT+TGG + tig0023416:49184-49203 None:intergenic 55.0%
Chromosome Type Strat End Strand Name
tig0023416 gene 48919 49853 48919 ID=Msa1408810;Name=Msa1408810
tig0023416 mRNA 48919 49853 48919 ID=Msa1408810-mRNA-1;Parent=Msa1408810;Name=Msa1408810-mRNA-1;_AED=0.08;_eAED=0.08;_QI=0|0|0|1|0.66|0.75|4|0|202
tig0023416 exon 49715 49853 49715 ID=Msa1408810-mRNA-1:exon:25738;Parent=Msa1408810-mRNA-1
tig0023416 exon 49435 49679 49435 ID=Msa1408810-mRNA-1:exon:25737;Parent=Msa1408810-mRNA-1
tig0023416 exon 49179 49261 49179 ID=Msa1408810-mRNA-1:exon:25736;Parent=Msa1408810-mRNA-1
tig0023416 exon 48919 49057 48919 ID=Msa1408810-mRNA-1:exon:25735;Parent=Msa1408810-mRNA-1
tig0023416 CDS 49715 49853 49715 ID=Msa1408810-mRNA-1:cds;Parent=Msa1408810-mRNA-1
tig0023416 CDS 49435 49679 49435 ID=Msa1408810-mRNA-1:cds;Parent=Msa1408810-mRNA-1
tig0023416 CDS 49179 49261 49179 ID=Msa1408810-mRNA-1:cds;Parent=Msa1408810-mRNA-1
tig0023416 CDS 48919 49057 48919 ID=Msa1408810-mRNA-1:cds;Parent=Msa1408810-mRNA-1
Gene Sequence

>Msa1408810

ATGCATCAGAGAAATACAAGGGCTATTACATGTCTTTTGTGCCGACATATGAGAAGAAGGCATGACACTGATTGTGAATTTGGTCAATACTTTTCGACCAGTAGGCATGCTGACTTTCAGTGTGTTATTCAAATTTGGGAACCACATCAAAGACAAATTGTTGCCGATTCGCTTCTCACAGAAGGAGAAGCTTGGCGAAGTAACCCAGATCGGGGTTTTCTTGGACACCAATTGGAGCTGGGTGCCCAAATAAGCACATCCTTGGATGAAATTGAAATTGCTAGAAGACTTTTAGCTTTTTGCATTGATAATGCAAATTCAAACAATGCACCTTCTTCCATGCCTTCTTGCACTCAAATTCCTGACTTAAACTTGAGTATATGGAGTACTGAACACATGGGTGACATGGACATCACGGAACAAGACATGCAAGGTATTCCATGTACTTGTGAAAGAGGAGAATCAAGTAATGCATCCAAGGGAATGGTGGCTGATGAAAAGGGAAAAGGAATTCTTAATGAACAAGGACCCACCAGTAATGAGGATGAAGACTTTTCCCAAAGTAAGATGGGGAATTTGAAGACTAGAAATATATATGAAATCCAT

Protein sequence

>Msa1408810

MHQRNTRAITCLLCRHMRRRHDTDCEFGQYFSTSRHADFQCVIQIWEPHQRQIVADSLLTEGEAWRSNPDRGFLGHQLELGAQISTSLDEIEIARRLLAFCIDNANSNNAPSSMPSCTQIPDLNLSIWSTEHMGDMDITEQDMQGIPCTCERGESSNASKGMVADEKGKGILNEQGPTSNEDEDFSQSKMGNLKTRNIYEIH