AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1409400


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1409400 MtrunA17_Chr7g0251181 92.035 113 8 1 41 153 18 129 8.18e-56 172
Msa1409400 MtrunA17_Chr8g0341671 73.723 137 33 2 1 136 10 144 1.29e-54 171
Msa1409400 MtrunA17_Chr8g0374111 64.198 81 29 0 1 81 17 97 4.31e-33 117
Msa1409400 MtrunA17_Chr2g0328021 59.000 100 35 2 5 99 54 152 2.68e-32 116
Msa1409400 MtrunA17_Chr4g0010151 46.457 127 51 4 4 115 74 198 5.25e-29 109
Msa1409400 MtrunA17_Chr8g0374251 76.364 55 13 0 10 64 65 119 6.78e-28 105
Msa1409400 MtrunA17_Chr4g0031411 54.023 87 39 1 8 94 70 155 8.80e-28 105
Msa1409400 MtrunA17_Chr7g0223311 80.000 55 11 0 14 68 78 132 1.85e-27 105
Msa1409400 MtrunA17_Chr4g0061761 79.245 53 11 0 11 63 38 90 8.42e-27 102
Msa1409400 MtrunA17_Chr2g0308011 47.967 123 62 2 13 134 37 158 8.55e-27 100
Msa1409400 MtrunA17_Chr4g0047001 60.000 80 30 1 14 91 56 135 1.87e-26 102
Msa1409400 MtrunA17_Chr7g0237921 49.167 120 49 4 8 116 70 188 1.98e-26 102
Msa1409400 MtrunA17_Chr4g0030051 71.429 63 16 1 2 64 32 92 2.01e-26 101
Msa1409400 MtrunA17_Chr8g0346551 48.485 99 35 2 10 108 21 103 2.49e-26 100
Msa1409400 MtrunA17_Chr5g0411951 69.643 56 17 0 8 63 38 93 4.37e-26 100
Msa1409400 MtrunA17_Chr6g0453321 57.971 69 29 0 13 81 136 204 4.74e-26 102
Msa1409400 MtrunA17_Chr3g0127201 67.188 64 19 2 8 70 21 83 2.37e-25 98.2
Msa1409400 MtrunA17_Chr2g0326001 71.186 59 16 1 6 64 34 91 6.53e-25 97.1
Msa1409400 MtrunA17_Chr8g0367161 59.459 74 28 1 13 84 32 105 7.15e-25 97.8
Msa1409400 MtrunA17_Chr7g0217581 62.903 62 23 0 15 76 234 295 7.66e-25 99.8
Msa1409400 MtrunA17_Chr2g0282511 79.592 49 10 0 13 61 45 93 8.94e-25 97.1
Msa1409400 MtrunA17_Chr3g0126201 64.179 67 20 1 15 77 53 119 2.02e-24 96.7
Msa1409400 MtrunA17_Chr2g0283811 67.925 53 17 0 13 65 47 99 2.22e-24 92.8
Msa1409400 MtrunA17_Chr1g0188851 63.934 61 22 0 7 67 37 97 3.34e-24 95.1
Msa1409400 MtrunA17_Chr3g0117601 71.429 56 16 0 8 63 56 111 3.53e-24 95.9
Msa1409400 MtrunA17_Chr7g0254161 50.685 73 36 0 13 85 101 173 3.64e-24 97.1
Msa1409400 MtrunA17_Chr2g0282441 66.102 59 16 1 6 64 22 76 1.13e-23 94.7
Msa1409400 MtrunA17_Chr1g0177251 67.742 62 18 1 15 76 14 73 1.14e-23 94.0
Msa1409400 MtrunA17_Chr3g0092111 60.000 65 26 0 13 77 136 200 2.02e-23 95.1
Msa1409400 MtrunA17_Chr4g0047761 69.811 53 16 0 11 63 70 122 2.76e-23 93.2
Msa1409400 MtrunA17_Chr5g0417551 51.087 92 37 2 13 96 127 218 3.45e-23 93.6
Msa1409400 MtrunA17_Chr2g0282051 70.370 54 15 1 11 64 44 96 3.58e-23 92.4
Msa1409400 MtrunA17_Chr8g0355261 60.000 60 23 1 5 64 111 169 4.39e-23 94.0
Msa1409400 MtrunA17_Chr5g0417561 50.549 91 37 2 13 95 119 209 5.48e-23 93.2
Msa1409400 MtrunA17_Chr5g0417601 61.905 63 24 0 15 77 142 204 8.71e-23 92.8
Msa1409400 MtrunA17_Chr4g0042481 58.065 62 26 0 13 74 122 183 1.03e-22 93.2
Msa1409400 MtrunA17_Chr5g0417571 59.322 59 24 0 6 64 131 189 1.27e-22 91.7
Msa1409400 MtrunA17_Chr6g0460731 48.649 74 38 0 13 86 96 169 1.95e-22 91.7
Msa1409400 MtrunA17_Chr6g0460721 46.512 86 40 1 13 92 89 174 8.69e-22 89.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1409400 AT1G51700.1 81.944 72 12 1 2 72 21 92 1.79e-37 127
Msa1409400 AT3G50410.1 69.863 73 18 2 1 69 14 86 1.28e-30 111
Msa1409400 AT5G66940.1 71.875 64 18 0 6 69 25 88 2.36e-30 110
Msa1409400 AT5G60850.1 62.963 81 26 1 9 89 47 123 7.17e-29 108
Msa1409400 AT3G45610.1 63.514 74 26 1 6 78 33 106 1.19e-28 106
Msa1409400 AT3G21270.1 80.702 57 10 1 13 68 29 85 1.61e-28 104
Msa1409400 AT1G07640.1 66.667 66 22 0 8 73 16 81 2.16e-28 106
Msa1409400 AT5G60200.1 55.056 89 38 1 6 92 48 136 4.20e-28 105
Msa1409400 AT1G07640.2 65.714 70 23 1 4 73 69 137 4.20e-28 106
Msa1409400 AT1G07640.3 66.667 66 22 0 8 73 80 145 5.45e-28 106
Msa1409400 AT5G65590.1 71.186 59 17 0 9 67 37 95 2.77e-27 104
Msa1409400 AT2G28810.2 68.966 58 18 0 7 64 71 128 6.86e-27 103
Msa1409400 AT2G28810.1 68.966 58 18 0 7 64 88 145 8.85e-27 103
Msa1409400 AT4G38000.1 58.824 85 33 2 6 89 33 116 1.41e-26 100
Msa1409400 AT1G21340.1 65.217 69 21 1 9 74 31 99 1.11e-25 99.0
Msa1409400 AT2G37590.1 66.667 63 20 1 2 64 79 140 2.66e-25 99.4
Msa1409400 AT3G55370.1 61.765 68 25 1 6 73 70 136 7.95e-25 97.8
Msa1409400 AT3G52440.1 48.421 95 42 1 15 109 27 114 8.31e-25 96.3
Msa1409400 AT3G55370.3 61.765 68 25 1 6 73 115 181 8.75e-25 98.6
Msa1409400 AT3G61850.2 50.000 92 37 2 10 99 59 143 8.98e-25 97.1
Msa1409400 AT3G61850.3 50.000 92 37 2 10 99 59 143 8.98e-25 97.1
Msa1409400 AT3G55370.2 61.765 68 25 1 6 73 70 136 9.04e-25 98.2
Msa1409400 AT2G28510.1 70.968 62 17 1 11 72 46 106 1.15e-24 96.7
Msa1409400 AT3G61850.1 50.000 92 37 2 10 99 71 155 1.22e-24 97.1
Msa1409400 AT3G61850.5 50.000 92 37 2 10 99 59 143 1.23e-24 97.1
Msa1409400 AT3G52440.2 48.421 95 42 1 15 109 46 133 1.23e-24 96.3
Msa1409400 AT1G28310.1 76.923 52 12 0 13 64 27 78 1.31e-24 97.1
Msa1409400 AT3G61850.4 50.000 92 37 2 10 99 71 155 1.41e-24 97.1
Msa1409400 AT1G28310.2 76.923 52 12 0 13 64 51 102 1.59e-24 97.4
Msa1409400 AT4G24060.1 68.966 58 17 1 8 64 47 104 2.45e-24 97.1
Msa1409400 AT3G47500.1 60.938 64 25 0 13 76 110 173 2.72e-24 97.8
Msa1409400 AT1G29160.1 69.231 52 16 0 13 64 62 113 2.77e-24 93.2
Msa1409400 AT5G02460.1 67.213 61 19 1 4 64 87 146 3.37e-24 97.1
Msa1409400 AT2G34140.1 69.231 52 16 0 13 64 58 109 3.93e-24 92.8
Msa1409400 AT1G69570.1 69.231 52 16 0 13 64 132 183 5.01e-24 96.7
Msa1409400 AT5G62940.1 71.429 56 16 0 8 63 68 123 1.05e-23 95.5
Msa1409400 AT2G46590.1 72.000 50 14 0 10 59 65 114 2.53e-23 94.4
Msa1409400 AT2G46590.2 72.000 50 14 0 10 59 77 126 2.84e-23 94.4
Msa1409400 AT4G00940.1 67.241 58 19 0 10 67 65 122 4.47e-23 92.8
Msa1409400 AT4G00940.3 67.241 58 19 0 10 67 65 122 4.47e-23 92.8
Msa1409400 AT4G00940.2 67.241 58 19 0 10 67 65 122 4.47e-23 92.8
Msa1409400 AT1G64620.1 69.643 56 16 1 8 62 43 98 5.43e-23 93.2
Msa1409400 AT1G47655.1 63.333 60 18 1 3 62 23 78 5.84e-23 90.5
Msa1409400 AT5G39660.3 61.290 62 24 0 13 74 138 199 6.20e-23 94.4
Msa1409400 AT5G39660.1 61.290 62 24 0 13 74 138 199 6.20e-23 94.4
Msa1409400 AT5G39660.2 61.290 62 24 0 13 74 138 199 6.20e-23 94.4
Msa1409400 AT4G21050.1 62.687 67 18 1 15 81 26 85 1.66e-22 89.4
Msa1409400 AT1G26790.1 65.385 52 18 0 13 64 111 162 4.24e-22 91.3
Msa1409400 AT5G62430.1 48.864 88 42 2 13 97 54 141 7.92e-22 89.4
Msa1409400 AT4G21040.1 58.462 65 21 2 15 79 27 85 2.49e-20 84.3
Msa1409400 AT4G21080.1 60.000 65 20 2 15 79 27 85 1.52e-19 82.4
Msa1409400 AT4G21030.1 61.364 44 17 0 15 58 23 66 9.66e-12 60.8

Find 34 sgRNAs with CRISPR-Local

Find 35 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGTTCTGGTTCTGGAAAATT+TGG 0.150101 tig0023519:+70387 Msa1409390:intron
ATCTGGGTCGGGTCAGTTTC+TGG 0.166656 tig0023519:+70099 Msa1409390:intron
GGACAAAAGGTGGTGCTTTA+AGG 0.182533 tig0023519:-70254 Msa1409400:CDS
AGTTGTTCTTGTTCTGGTTC+TGG 0.240682 tig0023519:+70378 Msa1409390:intron
GACCCGATTGTGAGAAATTT+TGG 0.247972 tig0023519:-70069 Msa1409400:CDS
CATTTGAGTTGTTCTTGTTC+TGG 0.278893 tig0023519:+70372 Msa1409390:intron
TTCTGGAAAATTTGGTTTCA+TGG 0.282386 tig0023519:+70395 Msa1409390:intron
CAATCGGGTCAACATGAATC+TGG 0.332686 tig0023519:+70082 Msa1409390:CDS
CACCTTACTCTTTGTCTTCG+TGG 0.369682 tig0023519:+70014 Msa1409390:CDS
TAGCTTCCGTGCACCTTCCT+TGG 0.374095 tig0023519:+69941 Msa1409390:CDS
TCACCAAAATTTCTCACAAT+CGG 0.384976 tig0023519:+70066 Msa1409390:CDS
TTCGTTCTGCATGTTATAAT+CGG 0.385397 tig0023519:-69982 Msa1409400:CDS
TTTCTTGTTCCAACACCAAC+TGG 0.415369 tig0023519:+70225 Msa1409390:CDS
TGCGAAAGATATTGGACAAA+AGG 0.445100 tig0023519:-70267 Msa1409400:CDS
CACCAAAATTTCTCACAATC+GGG 0.458876 tig0023519:+70067 Msa1409390:CDS
ACAAAGAGTAAGGTGGAGAG+TGG 0.467255 tig0023519:-70006 Msa1409400:CDS
TCTGGAAAATTTGGTTTCAT+GGG 0.482955 tig0023519:+70396 Msa1409390:intron
GAAGAAGTGGATGATGTTGA+TGG 0.514146 tig0023519:+70147 Msa1409390:CDS
GAGAATGCGGCCGTCGTCCA+AGG 0.526115 tig0023519:-69958 Msa1409400:CDS
GGTCAACATGAATCTGGGTC+GGG 0.544687 tig0023519:+70088 Msa1409390:intron
CACCACGAAGACAAAGAGTA+AGG 0.558695 tig0023519:-70016 Msa1409400:CDS
GGGTCAACATGAATCTGGGT+CGG 0.570528 tig0023519:+70087 Msa1409390:intron
AGGAACATACCAGTTGGTGT+TGG 0.571016 tig0023519:-70234 Msa1409400:CDS
AATCGGGTCAACATGAATCT+GGG 0.579145 tig0023519:+70083 Msa1409390:CDS
CGTGCACCTTCCTTGGACGA+CGG 0.582438 tig0023519:+69948 Msa1409390:CDS
ATGCGGCCGTCGTCCAAGGA+AGG 0.583986 tig0023519:-69954 Msa1409400:CDS
TGTTATAATCGGCGAGAATG+CGG 0.605263 tig0023519:-69971 Msa1409400:CDS
CTTACTCTTTGTCTTCGTGG+TGG 0.610675 tig0023519:+70017 Msa1409390:CDS
GTTGATGGAGAAGATGAAGA+TGG 0.610885 tig0023519:+70162 Msa1409390:CDS
GAAAGATATTGGACAAAAGG+TGG 0.625233 tig0023519:-70264 Msa1409400:CDS
GCTTTAAGGAACATACCAGT+TGG 0.625252 tig0023519:-70240 Msa1409400:CDS
CGTCGTCCAAGGAAGGTGCA+CGG 0.657720 tig0023519:-69947 Msa1409400:CDS
CACGAAGACAAAGAGTAAGG+TGG 0.663000 tig0023519:-70013 Msa1409400:CDS
GTGTTGCTTGATTCACAACG+TGG 0.731087 tig0023519:+70348 Msa1409390:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
GCAAAAATTGCGAAAGATAT+TGG - tig0023519:70048-70067 Msa1409400:CDS 30.0%
TCACCAAAATTTCTCACAAT+CGG + tig0023519:70260-70279 Msa1409390:intron 30.0%
!! ATTGTGAGAAATTTTGGTGA+AGG - tig0023519:70260-70279 Msa1409400:CDS 30.0%
TTCGTTCTGCATGTTATAAT+CGG - tig0023519:70341-70360 Msa1409400:CDS 30.0%
!! TGTTCTGGTTCTGGAAAATT+TGG + tig0023519:69939-69958 Msa1409390:CDS 35.0%
!! CATTTGAGTTGTTCTTGTTC+TGG + tig0023519:69954-69973 Msa1409390:CDS 35.0%
TGCGAAAGATATTGGACAAA+AGG - tig0023519:70056-70075 Msa1409400:CDS 35.0%
GAAAGATATTGGACAAAAGG+TGG - tig0023519:70059-70078 Msa1409400:CDS 35.0%
CACCAAAATTTCTCACAATC+GGG + tig0023519:70259-70278 Msa1409390:intron 35.0%
!! AGTTGTTCTTGTTCTGGTTC+TGG + tig0023519:69948-69967 Msa1409390:CDS 40.0%
!! GCTTTAAGGAACATACCAGT+TGG - tig0023519:70083-70102 Msa1409400:CDS 40.0%
TTTCTTGTTCCAACACCAAC+TGG + tig0023519:70101-70120 Msa1409390:intron 40.0%
! GTTGATGGAGAAGATGAAGA+TGG + tig0023519:70164-70183 Msa1409390:CDS 40.0%
GAAGAAGTGGATGATGTTGA+TGG + tig0023519:70179-70198 Msa1409390:CDS 40.0%
! AACTTTTGCTGCTGAAGAAG+TGG + tig0023519:70192-70211 Msa1409390:CDS 40.0%
AATCGGGTCAACATGAATCT+GGG + tig0023519:70243-70262 Msa1409390:intron 40.0%
! GACCCGATTGTGAGAAATTT+TGG - tig0023519:70254-70273 Msa1409400:CDS 40.0%
TGTTATAATCGGCGAGAATG+CGG - tig0023519:70352-70371 Msa1409400:CDS 40.0%
!! GTGTTGCTTGATTCACAACG+TGG + tig0023519:69978-69997 Msa1409390:CDS 45.0%
GGACAAAAGGTGGTGCTTTA+AGG - tig0023519:70069-70088 Msa1409400:CDS 45.0%
! AGGAACATACCAGTTGGTGT+TGG - tig0023519:70089-70108 Msa1409400:CDS 45.0%
CAATCGGGTCAACATGAATC+TGG + tig0023519:70244-70263 Msa1409390:intron 45.0%
CTTACTCTTTGTCTTCGTGG+TGG + tig0023519:70309-70328 Msa1409390:intron 45.0%
CACCACGAAGACAAAGAGTA+AGG - tig0023519:70307-70326 Msa1409400:CDS 45.0%
CACCTTACTCTTTGTCTTCG+TGG + tig0023519:70312-70331 Msa1409390:intron 45.0%
CACGAAGACAAAGAGTAAGG+TGG - tig0023519:70310-70329 Msa1409400:CDS 45.0%
ACAAAGAGTAAGGTGGAGAG+TGG - tig0023519:70317-70336 Msa1409400:CDS 45.0%
GGTCAACATGAATCTGGGTC+GGG + tig0023519:70238-70257 Msa1409390:intron 50.0%
GGGTCAACATGAATCTGGGT+CGG + tig0023519:70239-70258 Msa1409390:intron 50.0%
ATCTGGGTCGGGTCAGTTTC+TGG + tig0023519:70227-70246 Msa1409390:CDS 55.0%
TAGCTTCCGTGCACCTTCCT+TGG + tig0023519:70385-70404 Msa1409390:intron 55.0%
CGTGCACCTTCCTTGGACGA+CGG + tig0023519:70378-70397 Msa1409390:intron 60.0%
! CGTCGTCCAAGGAAGGTGCA+CGG - tig0023519:70376-70395 Msa1409400:CDS 60.0%
GAGAATGCGGCCGTCGTCCA+AGG - tig0023519:70365-70384 Msa1409400:CDS 65.0%
ATGCGGCCGTCGTCCAAGGA+AGG - tig0023519:70369-70388 Msa1409400:CDS 65.0%
Chromosome Type Strat End Strand Name
tig0023519 gene 69930 70415 69930 ID=Msa1409400;Name=Msa1409400
tig0023519 mRNA 69930 70415 69930 ID=Msa1409400-mRNA-1;Parent=Msa1409400;Name=Msa1409400-mRNA-1;_AED=0.03;_eAED=0.03;_QI=0|-1|0|1|-1|1|1|0|161
tig0023519 exon 69930 70415 69930 ID=Msa1409400-mRNA-1:exon:26022;Parent=Msa1409400-mRNA-1
tig0023519 CDS 69930 70415 69930 ID=Msa1409400-mRNA-1:cds;Parent=Msa1409400-mRNA-1
Gene Sequence

>Msa1409400

ATGAAACCAAATTTTCCAGAACCAGAACAAGAACAACTCAAATGTCCACGTTGTGAATCAAGCAACACAAAATTATGCTACTACAACAACTACAATCTCTCACAACGACGTCATTTTTGCAAAAATTGCGAAAGATATTGGACAAAAGGTGGTGCTTTAAGGAACATACCAGTTGGTGTTGGAACAAGAAAAGTTACAAAACGTTCATCAAATTCTAAACGTTTAACAACACCATCTTCATCTTCTCCATCAACATCATCCACTTCTTCAGCAGCAAAAGTTTCAGCTTCAGTTCCAGAAACTGACCCGACCCAGATTCATGTTGACCCGATTGTGAGAAATTTTGGTGAAGGTTTTCTTCTTACGCTTCTTAGCTCCACCACGAAGACAAAGAGTAAGGTGGAGAGTGGATTCGTTCTGCATGTTATAATCGGCGAGAATGCGGCCGTCGTCCAAGGAAGGTGCACGGAAGCTACGAGATCTTGA

Protein sequence

>Msa1409400

MKPNFPEPEQEQLKCPRCESSNTKLCYYNNYNLSQRRHFCKNCERYWTKGGALRNIPVGVGTRKVTKRSSNSKRLTTPSSSSPSTSSTSSAAKVSASVPETDPTQIHVDPIVRNFGEGFLLTLLSSTTKTKSKVESGFVLHVIIGENAAVVQGRCTEATRS*