Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1409900 | XP_013447966.1 | 89.545 | 220 | 23 | 0 | 1 | 220 | 1 | 220 | 1.25e-139 | 399 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1409900 | A0A072TYR6 | 89.545 | 220 | 23 | 0 | 1 | 220 | 1 | 220 | 5.98e-140 | 399 |
| Gene ID | Type | Classification |
|---|---|---|
| Msa1409900 | TF | C2H2 |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1409900 | MtrunA17_Chr7g0225091 | 89.545 | 220 | 23 | 0 | 1 | 220 | 1 | 220 | 1.15e-143 | 399 |
| Msa1409900 | MtrunA17_Chr7g0255511 | 81.197 | 234 | 30 | 1 | 1 | 220 | 1 | 234 | 3.35e-131 | 369 |
| Msa1409900 | MtrunA17_Chr7g0225121 | 71.795 | 234 | 30 | 2 | 1 | 220 | 1 | 212 | 1.67e-112 | 320 |
| Msa1409900 | MtrunA17_Chr7g0222361 | 74.257 | 202 | 37 | 1 | 34 | 220 | 1 | 202 | 1.42e-106 | 305 |
| Msa1409900 | MtrunA17_Chr7g0222371 | 66.942 | 121 | 25 | 1 | 19 | 124 | 1 | 121 | 3.66e-52 | 164 |
| Msa1409900 | MtrunA17_Chr4g0072571 | 30.714 | 140 | 78 | 4 | 83 | 220 | 47 | 169 | 6.83e-12 | 61.6 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 38 sgRNAs with CRISPR-Local
Find 40 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAGAAATGGAGAAATTTCTT+TGG | 0.293893 | tig0023577:+4589 | None:intergenic |
| CTCCTTCCTCTTGTTGCACA+TGG | 0.338528 | tig0023577:+4769 | None:intergenic |
| TATATATAAATCAAAGCTTA+AGG | 0.356106 | tig0023577:+4117 | None:intergenic |
| GGGTGTGGATATCAATATAA+TGG | 0.359691 | tig0023577:-4383 | Msa1409900:CDS |
| TGGTGCATTGCAACATATTC+CGG | 0.362580 | tig0023577:-4303 | Msa1409900:CDS |
| CACCATCACTCCATTTCATA+TGG | 0.364608 | tig0023577:-4405 | Msa1409900:CDS |
| CTTAAGGGTCAAATCAATCT+TGG | 0.396784 | tig0023577:+4133 | None:intergenic |
| AACAATATGATTCAACATGC+AGG | 0.449556 | tig0023577:-4356 | Msa1409900:CDS |
| TATCCACACCCCATATGAAA+TGG | 0.452870 | tig0023577:+4395 | None:intergenic |
| AGTAGACAATTTGAAGATGA+TGG | 0.466141 | tig0023577:-4729 | Msa1409900:CDS |
| TTCACTAGTCCACAGGCATT+AGG | 0.474635 | tig0023577:-4494 | Msa1409900:CDS |
| TTGGATTCATTGTTGATCAC+AGG | 0.479894 | tig0023577:-4562 | Msa1409900:CDS |
| TGAAGTTGGTAAAGTTTCAG+AGG | 0.484639 | tig0023577:-4216 | Msa1409900:CDS |
| ACTATGTCAAAGATTGAAGT+TGG | 0.488225 | tig0023577:-4230 | Msa1409900:CDS |
| ACCATCACTCCATTTCATAT+GGG | 0.489513 | tig0023577:-4404 | Msa1409900:CDS |
| TGGTGTTTAGACTTATCCCT+TGG | 0.491881 | tig0023577:+4423 | None:intergenic |
| TGTTAGTGATGAAGTTGATC+AGG | 0.506770 | tig0023577:-4186 | Msa1409900:CDS |
| CAAGCGAAAGGCAATTGTGC+TGG | 0.510108 | tig0023577:-4687 | Msa1409900:CDS |
| GAAGTTGGTAAAGTTTCAGA+GGG | 0.513957 | tig0023577:-4215 | Msa1409900:CDS |
| ATATATAAATCAAAGCTTAA+GGG | 0.520066 | tig0023577:+4118 | None:intergenic |
| ACTCCATTTCATATGGGGTG+TGG | 0.533278 | tig0023577:-4398 | Msa1409900:CDS |
| GGAGATAGATGAAGCGAATG+AGG | 0.566081 | tig0023577:-4282 | Msa1409900:CDS |
| TCATTCGCTTCATCTATCTC+CGG | 0.569516 | tig0023577:+4284 | None:intergenic |
| CAGAAAGTTCACTAGTCCAC+AGG | 0.575345 | tig0023577:-4501 | Msa1409900:CDS |
| ACAATATGATTCAACATGCA+GGG | 0.581032 | tig0023577:-4355 | Msa1409900:CDS |
| CTGATGCCATGTGCAACAAG+AGG | 0.598560 | tig0023577:-4775 | None:intergenic |
| TAGAATTCTGAGTTTCAACA+TGG | 0.602831 | tig0023577:+4657 | None:intergenic |
| TGCCATGTGCAACAAGAGGA+AGG | 0.602996 | tig0023577:-4771 | None:intergenic |
| GTTGGTAAAGTTTCAGAGGG+AGG | 0.624522 | tig0023577:-4212 | Msa1409900:CDS |
| CCATCACTCCATTTCATATG+GGG | 0.630153 | tig0023577:-4403 | Msa1409900:CDS |
| GGAAGACAAAGAGACTCAAG+AGG | 0.634668 | tig0023577:-4165 | Msa1409900:CDS |
| GTGCAACAAGAGGAAGGAGA+AGG | 0.635140 | tig0023577:-4765 | Msa1409900:CDS |
| CCCCATATGAAATGGAGTGA+TGG | 0.640487 | tig0023577:+4403 | None:intergenic |
| GAACAATGAGAACAAGCGAA+AGG | 0.645636 | tig0023577:-4699 | Msa1409900:CDS |
| ACTAGTCCACAGGCATTAGG+TGG | 0.661075 | tig0023577:-4491 | Msa1409900:CDS |
| TGATGACCACCTAATGCCTG+TGG | 0.664402 | tig0023577:+4485 | None:intergenic |
| AGAATTCTGAGTTTCAACAT+GGG | 0.673130 | tig0023577:+4658 | None:intergenic |
| AGACAAAGAGACTCAAGAGG+AGG | 0.673262 | tig0023577:-4162 | Msa1409900:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | AAGAAATGGAGAAATTTCTT+TGG | + | tig0023577:4308-4327 | None:intergenic | 25.0% |
| !! | ATTTCTCCATTTCTTCTTTT+TGG | - | tig0023577:4313-4332 | Msa1409900:CDS | 25.0% |
| ! | ATGAATCCAAAAAGAAGAAA+TGG | + | tig0023577:4322-4341 | None:intergenic | 25.0% |
| !! | TTTTATGCAGGAAATTTGTA+TGG | - | tig0023577:4571-4590 | Msa1409900:CDS | 25.0% |
| AGTAGACAATTTGAAGATGA+TGG | - | tig0023577:4165-4184 | Msa1409900:CDS | 30.0% | |
| AGAATTCTGAGTTTCAACAT+GGG | + | tig0023577:4239-4258 | None:intergenic | 30.0% | |
| TAGAATTCTGAGTTTCAACA+TGG | + | tig0023577:4240-4259 | None:intergenic | 30.0% | |
| AGTCTCAATAAAAAGATCCA+AGG | - | tig0023577:4454-4473 | Msa1409900:CDS | 30.0% | |
| GTCTCAATAAAAAGATCCAA+GGG | - | tig0023577:4455-4474 | Msa1409900:CDS | 30.0% | |
| AACAATATGATTCAACATGC+AGG | - | tig0023577:4538-4557 | Msa1409900:CDS | 30.0% | |
| ACAATATGATTCAACATGCA+GGG | - | tig0023577:4539-4558 | Msa1409900:CDS | 30.0% | |
| ACTATGTCAAAGATTGAAGT+TGG | - | tig0023577:4664-4683 | Msa1409900:CDS | 30.0% | |
| ! | CTTCTTCAGCAACATCTTTT+TGG | + | tig0023577:4270-4289 | None:intergenic | 35.0% |
| TTGGATTCATTGTTGATCAC+AGG | - | tig0023577:4332-4351 | Msa1409900:CDS | 35.0% | |
| ACCATCACTCCATTTCATAT+GGG | - | tig0023577:4490-4509 | Msa1409900:CDS | 35.0% | |
| GGGTGTGGATATCAATATAA+TGG | - | tig0023577:4511-4530 | Msa1409900:CDS | 35.0% | |
| !! | TGAAGTTGGTAAAGTTTCAG+AGG | - | tig0023577:4678-4697 | Msa1409900:CDS | 35.0% |
| !! | GAAGTTGGTAAAGTTTCAGA+GGG | - | tig0023577:4679-4698 | Msa1409900:CDS | 35.0% |
| ! | TGTTAGTGATGAAGTTGATC+AGG | - | tig0023577:4708-4727 | Msa1409900:CDS | 35.0% |
| CTTAAGGGTCAAATCAATCT+TGG | + | tig0023577:4764-4783 | None:intergenic | 35.0% | |
| GAACAATGAGAACAAGCGAA+AGG | - | tig0023577:4195-4214 | Msa1409900:CDS | 40.0% | |
| TGGTGTTTAGACTTATCCCT+TGG | + | tig0023577:4474-4493 | None:intergenic | 40.0% | |
| CACCATCACTCCATTTCATA+TGG | - | tig0023577:4489-4508 | Msa1409900:CDS | 40.0% | |
| CCATCACTCCATTTCATATG+GGG | - | tig0023577:4491-4510 | Msa1409900:CDS | 40.0% | |
| TATCCACACCCCATATGAAA+TGG | + | tig0023577:4502-4521 | None:intergenic | 40.0% | |
| ! | GGGTCATCAAACTTTTATGC+AGG | - | tig0023577:4559-4578 | Msa1409900:CDS | 40.0% |
| TGGTGCATTGCAACATATTC+CGG | - | tig0023577:4591-4610 | Msa1409900:CDS | 40.0% | |
| TCATTCGCTTCATCTATCTC+CGG | + | tig0023577:4613-4632 | None:intergenic | 40.0% | |
| CAGAAAGTTCACTAGTCCAC+AGG | - | tig0023577:4393-4412 | Msa1409900:CDS | 45.0% | |
| TTCACTAGTCCACAGGCATT+AGG | - | tig0023577:4400-4419 | Msa1409900:CDS | 45.0% | |
| CCCCATATGAAATGGAGTGA+TGG | + | tig0023577:4494-4513 | None:intergenic | 45.0% | |
| ACTCCATTTCATATGGGGTG+TGG | - | tig0023577:4496-4515 | Msa1409900:CDS | 45.0% | |
| GGAGATAGATGAAGCGAATG+AGG | - | tig0023577:4612-4631 | Msa1409900:CDS | 45.0% | |
| ! | GTTGGTAAAGTTTCAGAGGG+AGG | - | tig0023577:4682-4701 | Msa1409900:CDS | 45.0% |
| GGAAGACAAAGAGACTCAAG+AGG | - | tig0023577:4729-4748 | Msa1409900:CDS | 45.0% | |
| AGACAAAGAGACTCAAGAGG+AGG | - | tig0023577:4732-4751 | Msa1409900:CDS | 45.0% | |
| GTGCAACAAGAGGAAGGAGA+AGG | - | tig0023577:4129-4148 | Msa1409900:CDS | 50.0% | |
| CAAGCGAAAGGCAATTGTGC+TGG | - | tig0023577:4207-4226 | Msa1409900:CDS | 50.0% | |
| ACTAGTCCACAGGCATTAGG+TGG | - | tig0023577:4403-4422 | Msa1409900:CDS | 50.0% | |
| TGATGACCACCTAATGCCTG+TGG | + | tig0023577:4412-4431 | None:intergenic | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| tig0023577 | gene | 4127 | 4789 | 4127 | ID=Msa1409900;Name=Msa1409900 |
| tig0023577 | mRNA | 4127 | 4789 | 4127 | ID=Msa1409900-mRNA-1;Parent=Msa1409900;Name=Msa1409900-mRNA-1;_AED=0.00;_eAED=0.00;_QI=0|-1|0|1|-1|1|1|0|220 |
| tig0023577 | exon | 4127 | 4789 | 4127 | ID=Msa1409900-mRNA-1:exon:26305;Parent=Msa1409900-mRNA-1 |
| tig0023577 | CDS | 4127 | 4789 | 4127 | ID=Msa1409900-mRNA-1:cds;Parent=Msa1409900-mRNA-1 |
| Gene Sequence |
| Protein sequence |
