AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1411630

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1411630 MtrunA17_Chr4g0063401 94.355 248 13 1 1 247 1 248 2.79e-177 490
Msa1411630 MtrunA17_Chr4g0063361 64.173 254 70 5 1 246 1 241 1.12e-115 334
Msa1411630 MtrunA17_Chr4g0063391 56.800 250 66 7 1 248 1 210 3.41e-87 261
Msa1411630 MtrunA17_Chr4g0063371 47.130 331 68 9 1 325 1 230 5.59e-75 230
Msa1411630 MtrunA17_Chr3g0098251 57.635 203 54 5 44 246 7 177 9.51e-74 226
Msa1411630 MtrunA17_Chr8g0360671 54.777 157 41 4 99 255 41 167 2.28e-47 157
Msa1411630 MtrunA17_Chr8g0366501 34.106 302 140 10 71 326 86 374 4.97e-41 147
Msa1411630 MtrunA17_Chr4g0063411 89.024 82 9 0 245 326 9 90 1.98e-33 118
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1411630 AT3G19360.1 44.753 324 131 10 42 326 59 373 1.42e-76 240
Msa1411630 AT3G19360.2 44.753 324 131 10 42 326 59 373 1.42e-76 240
Msa1411630 AT1G32360.1 32.550 298 159 8 71 326 85 382 1.93e-36 135
Msa1411630 AT2G35430.1 30.392 204 108 8 138 326 66 250 2.50e-16 77.8
Msa1411630 AT2G35430.1 35.849 106 46 4 77 168 71 168 2.50e-11 63.5

Find 101 sgRNAs with CRISPR-Local

Find 213 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATTGGTTCTCCAAAGTCTTT+TGG 0.221074 tig0023824:-39795 Msa1411630:CDS
GCAATAAGCATTGGAAATAT+TGG 0.228785 tig0023824:-39813 Msa1411630:CDS
ATGAGGATCCTGCTAGGTTT+AGG 0.244285 tig0023824:-39866 Msa1411630:CDS
TGGGGATGGTTCTAACAATT+TGG 0.253072 tig0023824:-39775 Msa1411630:CDS
CTAACAATTTGGAAGGCATT+AGG 0.254194 tig0023824:-39764 Msa1411630:CDS
AAGAAGATCAATGGTATTTA+TGG 0.269407 tig0023824:-37556 Msa1411630:CDS
CACTTACTTAGGTTGAAATT+AGG 0.275359 tig0023824:-37580 Msa1411630:CDS
CAAGGCAGCCACCACCAAAT+TGG 0.283685 tig0023824:-39983 Msa1411630:CDS
TGAGGATCCTGCTAGGTTTA+GGG 0.310738 tig0023824:-39865 Msa1411630:CDS
CTAGGTTTAGGGATGATTCT+TGG 0.317545 tig0023824:-39854 Msa1411630:CDS
GCAACACCTGTTGAGGCTTT+TGG 0.324209 tig0023824:+37673 None:intergenic
TTCCAATAAGCGCTCCTTGC+TGG 0.328577 tig0023824:+39729 None:intergenic
AGCCAGCAAGGAGCGCTTAT+TGG 0.331978 tig0023824:-39731 Msa1411630:CDS
TCAATGGTATTTATGGTGAT+TGG 0.333200 tig0023824:-37549 Msa1411630:CDS
CACTTCAATGATATCGTTGT+TGG 0.339985 tig0023824:+40310 None:intergenic
ATACAGGGAGCTGTCCCTTT+GGG 0.341839 tig0023824:-39683 Msa1411630:CDS
ACCTGAACTTAGTGCAGATT+CGG 0.365276 tig0023824:+40129 None:intergenic
TAACAATTTGGAAGGCATTA+GGG 0.381170 tig0023824:-39763 Msa1411630:CDS
TAAGGCAGCCACCGCGGAAT+TGG 0.383871 tig0023824:-40052 Msa1411630:CDS
ATGCTGCTTGTTCCTCGGTT+TGG 0.390724 tig0023824:+40164 None:intergenic
TCAGAGTGATCGTTATTGAT+TGG 0.398911 tig0023824:+40275 None:intergenic
CAGAGTGATCGTTATTGATT+GGG 0.400279 tig0023824:+40276 None:intergenic
AACGGTGAGGAATGTCCTTA+TGG 0.402090 tig0023824:-39909 Msa1411630:CDS
AGGTTTGGTACTTGTAGAAA+TGG 0.405516 tig0023824:-40110 Msa1411630:CDS
CCCGAATCTGCACTAAGTTC+AGG 0.412306 tig0023824:-40130 Msa1411630:CDS
ATCTGCACTAAGTTCAGGTT+TGG 0.426557 tig0023824:-40125 Msa1411630:CDS
CCTGAACTTAGTGCAGATTC+GGG 0.429469 tig0023824:+40130 None:intergenic
CATACAGGGAGCTGTCCCTT+TGG 0.435906 tig0023824:-39684 Msa1411630:CDS
GATTGATATGCCCTCCTTCA+AGG 0.436742 tig0023824:-40202 Msa1411630:CDS
CTGCTTGTTCCTCGGTTTGG+TGG 0.444819 tig0023824:+40167 None:intergenic
GCAGCCACCGCGGAATTGGC+AGG 0.444919 tig0023824:-40048 Msa1411630:CDS
AAGGCAGCCACCACCAAATT+GGG 0.448398 tig0023824:-39982 Msa1411630:CDS
CTGTGTCAATGGCAGCATAC+AGG 0.450029 tig0023824:-39699 Msa1411630:CDS
GAACCATCCCCAAAAGACTT+TGG 0.450065 tig0023824:+39786 None:intergenic
CTTGTTCCTCGGTTTGGTGG+AGG 0.456539 tig0023824:+40170 None:intergenic
CACTTTGCTCATGGAGAAGC+AGG 0.457533 tig0023824:-39651 Msa1411630:intron
ATAAGAAGAAGCACTTACTT+AGG 0.466516 tig0023824:-37591 Msa1411630:CDS
AGCAATTCTCGCCCTCTTGA+AGG 0.471550 tig0023824:+40244 None:intergenic
AATATCGTCTGGAAAATTCA+TGG 0.472650 tig0023824:+40370 None:intergenic
CTACAGGTTGGAATATCGTC+TGG 0.476700 tig0023824:+40359 None:intergenic
AAAATTGCAATTACGCTCAC+GGG 0.487194 tig0023824:-40085 Msa1411630:CDS
ACTTCACCAAAAGCCTCAAC+AGG 0.492958 tig0023824:-37679 Msa1411630:CDS
TCGTTATTGATTGGGATTTG+AGG 0.495130 tig0023824:+40284 None:intergenic
GGAGGCTGAATCATCCTTGA+AGG 0.501461 tig0023824:+40188 None:intergenic
GAGACCTGTCACTTTGCTCA+TGG 0.502581 tig0023824:-39660 Msa1411630:CDS
CTCACGGGGCTGATGAGATA+AGG 0.504892 tig0023824:-40070 Msa1411630:CDS
TTGATCCTCGCAACGACGAA+CGG 0.512205 tig0023824:-40019 Msa1411630:CDS
CATGACAAAGGAAGAGGAGC+AGG 0.513127 tig0023824:-37614 Msa1411630:CDS
TGATCTTCATCCCAATTTGG+TGG 0.514081 tig0023824:+39972 None:intergenic
GAGTTACAAGCTCCTGGTGG+AGG 0.514447 tig0023824:-37757 Msa1411630:CDS
AAGCTACACTTATCACCATA+AGG 0.515098 tig0023824:+39894 None:intergenic
GAAGGTAGGAGTAGAATCAT+TGG 0.517961 tig0023824:+37651 None:intergenic
CTTTGTCATGAGTAGACTTG+TGG 0.519083 tig0023824:+37627 None:intergenic
TTCTCCATGAGCAAAGTGAC+AGG 0.523166 tig0023824:+39656 None:intergenic
TTCTTCATGAGGATCCTGCT+AGG 0.523992 tig0023824:-39872 Msa1411630:CDS
GGAAGACAAAGCTGTGTCAA+TGG 0.532349 tig0023824:-39710 Msa1411630:CDS
ATCATTGGCAACACCTGTTG+AGG 0.534873 tig0023824:+37666 None:intergenic
GAATCATCCCTAAACCTAGC+AGG 0.537317 tig0023824:+39858 None:intergenic
TCTTCATCCCAATTTGGTGG+TGG 0.537541 tig0023824:+39975 None:intergenic
GATGGTTCTAACAATTTGGA+AGG 0.539743 tig0023824:-39771 Msa1411630:CDS
GAGTGTTCTGCAATAAGCAT+TGG 0.542316 tig0023824:-39822 Msa1411630:CDS
AAGTGACAGGTCTCCCCAAA+GGG 0.549845 tig0023824:+39669 None:intergenic
TGTGTCAATGGCAGCATACA+GGG 0.552109 tig0023824:-39698 Msa1411630:CDS
CTGCGGTGGTGACATCCTAC+AGG 0.555913 tig0023824:+40343 None:intergenic
GAAAATTGCAATTACGCTCA+CGG 0.559384 tig0023824:-40086 Msa1411630:CDS
AAATCAGAGTTACAAGCTCC+TGG 0.561857 tig0023824:-37763 Msa1411630:intron
AAATTAGGCAAGAAGATCAA+TGG 0.564267 tig0023824:-37565 Msa1411630:CDS
AGTAGACTTGTGGTGAAGGT+AGG 0.573300 tig0023824:+37637 None:intergenic
CAACGACGAACGGCTTCAGA+TGG 0.574258 tig0023824:-40009 Msa1411630:CDS
TCAGCTTCAATGCCTCCACC+AGG 0.581762 tig0023824:+37745 None:intergenic
GGCTGAATCATCCTTGAAGG+AGG 0.587591 tig0023824:+40191 None:intergenic
TCTACTCATGACAAAGGAAG+AGG 0.589668 tig0023824:-37620 Msa1411630:CDS
CTCGCCCTCTTGAAGGAATG+TGG 0.590307 tig0023824:+40251 None:intergenic
TACAGGGAGCTGTCCCTTTG+GGG 0.596640 tig0023824:-39682 Msa1411630:CDS
CATGAGTAGACTTGTGGTGA+AGG 0.601665 tig0023824:+37633 None:intergenic
GGAGGGCATATCAATCCACT+AGG 0.602999 tig0023824:+40209 None:intergenic
CTGAGCCACATTCCTTCAAG+AGG 0.603957 tig0023824:-40256 Msa1411630:CDS
GGGCTGTGAACAAGCCAGCA+AGG 0.614710 tig0023824:-39743 Msa1411630:CDS
TAAGTGTAGCTTTCTTCATG+AGG 0.621800 tig0023824:-39883 Msa1411630:CDS
TGAAGCCGTTCGTCGTTGCG+AGG 0.623112 tig0023824:+40014 None:intergenic
AAAGTGACAGGTCTCCCCAA+AGG 0.626382 tig0023824:+39668 None:intergenic
CAGACGATATTCCAACCTGT+AGG 0.630277 tig0023824:-40358 Msa1411630:CDS
AGCTCCTGCCAATTCCGCGG+TGG 0.631980 tig0023824:+40044 None:intergenic
CACAAGTCTACTCATGACAA+AGG 0.632619 tig0023824:-37626 Msa1411630:CDS
GAGGGCATATCAATCCACTA+GGG 0.647490 tig0023824:+40210 None:intergenic
AACGGCTTCAGATGGAGACA+AGG 0.650858 tig0023824:-40001 Msa1411630:CDS
GCTGAATCATCCTTGAAGGA+GGG 0.652254 tig0023824:+40192 None:intergenic
TGAGCCACATTCCTTCAAGA+GGG 0.655888 tig0023824:-40255 Msa1411630:CDS
ACTAGCTCCTGCCAATTCCG+CGG 0.663260 tig0023824:+40041 None:intergenic
GGTGGTGACATCCTACAGGT+TGG 0.681473 tig0023824:+40347 None:intergenic
ATGAGATAAGGCAGCCACCG+CGG 0.683865 tig0023824:-40058 Msa1411630:CDS
TCAGAGTTACAAGCTCCTGG+TGG 0.696701 tig0023824:-37760 Msa1411630:intron
GGGCATATCAATCCACTAGG+GGG 0.701239 tig0023824:+40212 None:intergenic
ACAACGATATCATTGAAGTG+AGG 0.706867 tig0023824:-40307 Msa1411630:CDS
ATTCAGCCTCCACCAAACCG+AGG 0.710090 tig0023824:-40176 Msa1411630:CDS
CAAGAAGTATTACAACGGTG+AGG 0.717786 tig0023824:-39922 Msa1411630:CDS
AAATTGCAATTACGCTCACG+GGG 0.722741 tig0023824:-40084 Msa1411630:CDS
AGGGCATATCAATCCACTAG+GGG 0.727577 tig0023824:+40211 None:intergenic
CTGTGCAAGAAGTATTACAA+CGG 0.729247 tig0023824:-39927 Msa1411630:CDS
TTGGATGAAGCAAACTGCGG+TGG 0.740311 tig0023824:+40329 None:intergenic
TTGTTGGATGAAGCAAACTG+CGG 0.811729 tig0023824:+40326 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATAATTTTTTTTTGCATATT+TGG - tig0023824:40021-40040 Msa1411630:CDS 10.0%
!! GTGATTGAATATTTAAATTT+AGG + tig0023824:39067-39086 None:intergenic 15.0%
!!! TTCTAATTCAAAAGTAATTT+TGG + tig0023824:39295-39314 None:intergenic 15.0%
!! CAAAACCATTAATCAAAATA+GGG + tig0023824:38315-38334 None:intergenic 20.0%
!! TCAAAACCATTAATCAAAAT+AGG + tig0023824:38316-38335 None:intergenic 20.0%
!! AAATAAAAATTCCTGACAAT+GGG + tig0023824:39024-39043 None:intergenic 20.0%
!! AATCATCCATTATAATATGA+AGG + tig0023824:39089-39108 None:intergenic 20.0%
!!! TTATATGTCATGCTTATTTT+TGG - tig0023824:39494-39513 Msa1411630:intron 20.0%
!! ACAATTACAATAGATAAGAA+AGG - tig0023824:40048-40067 Msa1411630:CDS 20.0%
! ATTAATCAAAATAGGGAGAA+TGG + tig0023824:38308-38327 None:intergenic 25.0%
!!! ATTCTCCCTATTTTGATTAA+TGG - tig0023824:38307-38326 Msa1411630:intron 25.0%
!!! TTCTTTCAGTTTTGAAGTAA+TGG - tig0023824:38573-38592 Msa1411630:intron 25.0%
! AAAAATCTGGTATAGAAACA+TGG + tig0023824:38726-38745 None:intergenic 25.0%
!!! ATGTTTCTATACCAGATTTT+TGG - tig0023824:38725-38744 Msa1411630:intron 25.0%
! AATAAAAATTCCTGACAATG+GGG + tig0023824:39023-39042 None:intergenic 25.0%
! CAAATAAAAATTCCTGACAA+TGG + tig0023824:39025-39044 None:intergenic 25.0%
! CAATCACCTTCATATTATAA+TGG - tig0023824:39080-39099 Msa1411630:intron 25.0%
!!! AAGTAATTTTGGTACATACT+TGG + tig0023824:39284-39303 None:intergenic 25.0%
! AATGTTTGACCGTAAAATTA+AGG + tig0023824:39883-39902 None:intergenic 25.0%
! TTATAACAATTGAAGCTGTT+TGG - tig0023824:40074-40093 Msa1411630:CDS 25.0%
! AAGAAGATCAATGGTATTTA+TGG - tig0023824:40349-40368 Msa1411630:CDS 25.0%
! TTTTGATCTTCATCCCAATT+TGG + tig0023824:37939-37958 None:intergenic 30.0%
!! GCAATAAGCATTGGAAATAT+TGG - tig0023824:38092-38111 Msa1411630:intron 30.0%
!! TAACAATTTGGAAGGCATTA+GGG - tig0023824:38142-38161 Msa1411630:intron 30.0%
TTCTATGATGATTGTGTAGA+AGG - tig0023824:38380-38399 Msa1411630:intron 30.0%
TGTTGTGTATGATATTTCCA+TGG - tig0023824:38461-38480 Msa1411630:intron 30.0%
ATATCATACACAACAACGAT+AGG + tig0023824:38554-38573 None:intergenic 30.0%
TCTTAGTGTTGTCAAATTCA+TGG - tig0023824:38628-38647 Msa1411630:intron 30.0%
! TATCCTAAAAAGCATATCAC+TGG - tig0023824:38893-38912 Msa1411630:intron 30.0%
TTGTTAAATAGCGACTATAG+CGG + tig0023824:38957-38976 None:intergenic 30.0%
! AATGGATGATTGCTATTGTA+TGG - tig0023824:39098-39117 Msa1411630:intron 30.0%
AATCAACAAATGTAAAGCTG+TGG + tig0023824:39146-39165 None:intergenic 30.0%
! CTTTACATTTGTTGATTGAC+TGG - tig0023824:39149-39168 Msa1411630:intron 30.0%
!! GATACACTAGGATAGTTTTA+CGG - tig0023824:39334-39353 Msa1411630:intron 30.0%
ATGATACCAAGTACGAATAT+CGG + tig0023824:39591-39610 None:intergenic 30.0%
AAAGATAAAAGCAGTCACTT+CGG + tig0023824:39655-39674 None:intergenic 30.0%
TGTCCGACTCATAAAAAAAT+TGG - tig0023824:39809-39828 Msa1411630:CDS 30.0%
!!! CCTCCAATTTTTTTATGAGT+CGG + tig0023824:39815-39834 None:intergenic 30.0%
AATATAAAACCTCGCAAATG+AGG + tig0023824:39844-39863 None:intergenic 30.0%
! TGTCGCAATCCTTAATTTTA+CGG - tig0023824:39871-39890 Msa1411630:CDS 30.0%
! TCAAACATTTTTAATGCAGC+TGG - tig0023824:39894-39913 Msa1411630:CDS 30.0%
!! ATAAGAAGAAGCACTTACTT+AGG - tig0023824:40314-40333 Msa1411630:CDS 30.0%
!! CACTTACTTAGGTTGAAATT+AGG - tig0023824:40325-40344 Msa1411630:CDS 30.0%
AAATTAGGCAAGAAGATCAA+TGG - tig0023824:40340-40359 Msa1411630:CDS 30.0%
TCAATGGTATTTATGGTGAT+TGG - tig0023824:40356-40375 Msa1411630:CDS 30.0%
CACTTCAATGATATCGTTGT+TGG + tig0023824:37598-37617 None:intergenic 35.0%
ACAACGATATCATTGAAGTG+AGG - tig0023824:37598-37617 Msa1411630:CDS 35.0%
! TCGTTATTGATTGGGATTTG+AGG + tig0023824:37624-37643 None:intergenic 35.0%
! CAGAGTGATCGTTATTGATT+GGG + tig0023824:37632-37651 None:intergenic 35.0%
TCAGAGTGATCGTTATTGAT+TGG + tig0023824:37633-37652 None:intergenic 35.0%
! AAAAAATGCTGCTTGTTCCT+CGG + tig0023824:37749-37768 None:intergenic 35.0%
! AGGTTTGGTACTTGTAGAAA+TGG - tig0023824:37795-37814 Msa1411630:intron 35.0%
GAAAATTGCAATTACGCTCA+CGG - tig0023824:37819-37838 Msa1411630:intron 35.0%
AAAATTGCAATTACGCTCAC+GGG - tig0023824:37820-37839 Msa1411630:intron 35.0%
!! CTGTGCAAGAAGTATTACAA+CGG - tig0023824:37978-37997 Msa1411630:intron 35.0%
AAGCTACACTTATCACCATA+AGG + tig0023824:38014-38033 None:intergenic 35.0%
TAAGTGTAGCTTTCTTCATG+AGG - tig0023824:38022-38041 Msa1411630:intron 35.0%
!! ATTGGTTCTCCAAAGTCTTT+TGG - tig0023824:38110-38129 Msa1411630:intron 35.0%
!! TTGGTTCTCCAAAGTCTTTT+GGG - tig0023824:38111-38130 Msa1411630:intron 35.0%
!! GATGGTTCTAACAATTTGGA+AGG - tig0023824:38134-38153 Msa1411630:intron 35.0%
!! CTAACAATTTGGAAGGCATT+AGG - tig0023824:38141-38160 Msa1411630:intron 35.0%
ATCATACACAACAACGAAAG+TGG + tig0023824:38454-38473 None:intergenic 35.0%
GTGTATGATATTTCCATGGT+TGG - tig0023824:38465-38484 Msa1411630:intron 35.0%
AGTTTATGTCAAACCAACCA+TGG + tig0023824:38481-38500 None:intergenic 35.0%
! GGAAAACTTGTTTGTACGAT+CGG - tig0023824:38594-38613 Msa1411630:intron 35.0%
TATGGTAGCTGGAACATAAT+CGG + tig0023824:38654-38673 None:intergenic 35.0%
! TTATGATAATGCTGCCCTAT+TGG - tig0023824:38773-38792 Msa1411630:intron 35.0%
ATACACAGTGTTGTCAATTG+TGG - tig0023824:38800-38819 Msa1411630:intron 35.0%
AGCTATTACACTGTTGCATA+GGG + tig0023824:38856-38875 None:intergenic 35.0%
!! TGTCCAGTGATATGCTTTTT+AGG + tig0023824:38899-38918 None:intergenic 35.0%
TGTTAAATAGCGACTATAGC+GGG + tig0023824:38956-38975 None:intergenic 35.0%
! GACTGGAACTTTTAGATGTT+TGG - tig0023824:39166-39185 Msa1411630:intron 35.0%
TTGTATCCGATATTCGTACT+TGG - tig0023824:39582-39601 Msa1411630:intron 35.0%
TAACCTTATCCATGCAACAT+AGG - tig0023824:39612-39631 Msa1411630:intron 35.0%
GAGTCGGACATTAAATTTAC+AGG + tig0023824:39799-39818 None:intergenic 35.0%
CCGACTCATAAAAAAATTGG+AGG - tig0023824:39812-39831 Msa1411630:CDS 35.0%
TGTCAAAAACCTTAACGTCT+TGG - tig0023824:39941-39960 Msa1411630:CDS 35.0%
! GGTTTCCATGTATTTTGTTC+TGG - tig0023824:40095-40114 Msa1411630:CDS 35.0%
ATCAGCCAGAACAAAATACA+TGG + tig0023824:40103-40122 None:intergenic 35.0%
! AGCTTTTGTTGAGCTTACTA+TGG + tig0023824:40197-40216 None:intergenic 35.0%
TTGTTGGATGAAGCAAACTG+CGG + tig0023824:37582-37601 None:intergenic 40.0%
ACCTGAACTTAGTGCAGATT+CGG + tig0023824:37779-37798 None:intergenic 40.0%
! ATCTGCACTAAGTTCAGGTT+TGG - tig0023824:37780-37799 Msa1411630:intron 40.0%
AAATTGCAATTACGCTCACG+GGG - tig0023824:37821-37840 Msa1411630:intron 40.0%
TGATCTTCATCCCAATTTGG+TGG + tig0023824:37936-37955 None:intergenic 40.0%
!! CAAGAAGTATTACAACGGTG+AGG - tig0023824:37983-38002 Msa1411630:intron 40.0%
CTAGGTTTAGGGATGATTCT+TGG - tig0023824:38051-38070 Msa1411630:intron 40.0%
GAGTGTTCTGCAATAAGCAT+TGG - tig0023824:38083-38102 Msa1411630:intron 40.0%
! TGGTTCTCCAAAGTCTTTTG+GGG - tig0023824:38112-38131 Msa1411630:intron 40.0%
!! TGGGGATGGTTCTAACAATT+TGG - tig0023824:38130-38149 Msa1411630:intron 40.0%
CATACACAACAACGATAGGA+AGG + tig0023824:38550-38569 None:intergenic 40.0%
GATTATGTTCCAGCTACCAT+AGG - tig0023824:38653-38672 Msa1411630:intron 40.0%
ATAATAGCCATTGCAGCCTA+TGG + tig0023824:38672-38691 None:intergenic 40.0%
GCTGCAATGGCTATTATATC+TGG - tig0023824:38675-38694 Msa1411630:intron 40.0%
GCATTATCATAAGTGGAAGG+TGG + tig0023824:38765-38784 None:intergenic 40.0%
GCAGCATTATCATAAGTGGA+AGG + tig0023824:38768-38787 None:intergenic 40.0%
TAGGGCAGCATTATCATAAG+TGG + tig0023824:38772-38791 None:intergenic 40.0%
CACTGTGTATACCTCCAATA+GGG + tig0023824:38790-38809 None:intergenic 40.0%
ACACTGTGTATACCTCCAAT+AGG + tig0023824:38791-38810 None:intergenic 40.0%
CAGCTATTACACTGTTGCAT+AGG + tig0023824:38857-38876 None:intergenic 40.0%
GTTAAATAGCGACTATAGCG+GGG + tig0023824:38955-38974 None:intergenic 40.0%
CTGCATAGTCGATCAAAAGA+AGG + tig0023824:39211-39230 None:intergenic 40.0%
! TTTTGATCGACTATGCAGCA+TGG - tig0023824:39213-39232 Msa1411630:intron 40.0%
TCACACTCTTACTGTACTTG+TGG - tig0023824:39251-39270 Msa1411630:intron 40.0%
ATACTCCACTTGGATACACT+AGG - tig0023824:39322-39341 Msa1411630:intron 40.0%
ACTATCCTAGTGTATCCAAG+TGG + tig0023824:39330-39349 None:intergenic 40.0%
ACACCTATGTTGCATGGATA+AGG + tig0023824:39618-39637 None:intergenic 40.0%
TATCCATGCAACATAGGTGT+CGG - tig0023824:39618-39637 Msa1411630:intron 40.0%
! TCCAATCCTGTTTCGTCTTT+TGG - tig0023824:39757-39776 Msa1411630:CDS 40.0%
ACCAAAAGACGAAACAGGAT+TGG + tig0023824:39761-39780 None:intergenic 40.0%
CAGAAACCAAAAGACGAAAC+AGG + tig0023824:39766-39785 None:intergenic 40.0%
!!! CGTCTTTTGGTTTCTGCTAA+TGG - tig0023824:39770-39789 Msa1411630:CDS 40.0%
AAGGTATGCGACAAAAACTG+TGG + tig0023824:39967-39986 None:intergenic 40.0%
! TGTTCTGGCTGATGAAGTAT+TGG - tig0023824:40110-40129 Msa1411630:CDS 40.0%
AAATCAGAGTTACAAGCTCC+TGG - tig0023824:40142-40161 Msa1411630:CDS 40.0%
GAAGGTAGGAGTAGAATCAT+TGG + tig0023824:40257-40276 None:intergenic 40.0%
CTTTGTCATGAGTAGACTTG+TGG + tig0023824:40281-40300 None:intergenic 40.0%
CACAAGTCTACTCATGACAA+AGG - tig0023824:40279-40298 Msa1411630:CDS 40.0%
TCTACTCATGACAAAGGAAG+AGG - tig0023824:40285-40304 Msa1411630:CDS 40.0%
! CTACAGGTTGGAATATCGTC+TGG + tig0023824:37549-37568 None:intergenic 45.0%
CAGACGATATTCCAACCTGT+AGG - tig0023824:37547-37566 Msa1411630:CDS 45.0%
TGAGCCACATTCCTTCAAGA+GGG - tig0023824:37650-37669 Msa1411630:CDS 45.0%
AGGGCATATCAATCCACTAG+GGG + tig0023824:37697-37716 None:intergenic 45.0%
! GAGGGCATATCAATCCACTA+GGG + tig0023824:37698-37717 None:intergenic 45.0%
GATTGATATGCCCTCCTTCA+AGG - tig0023824:37703-37722 Msa1411630:CDS 45.0%
GCTGAATCATCCTTGAAGGA+GGG + tig0023824:37716-37735 None:intergenic 45.0%
CCTGAACTTAGTGCAGATTC+GGG + tig0023824:37778-37797 None:intergenic 45.0%
! TCTTCATCCCAATTTGGTGG+TGG + tig0023824:37933-37952 None:intergenic 45.0%
AACGGTGAGGAATGTCCTTA+TGG - tig0023824:37996-38015 Msa1411630:intron 45.0%
TTCTTCATGAGGATCCTGCT+AGG - tig0023824:38033-38052 Msa1411630:intron 45.0%
ATGAGGATCCTGCTAGGTTT+AGG - tig0023824:38039-38058 Msa1411630:intron 45.0%
TGAGGATCCTGCTAGGTTTA+GGG - tig0023824:38040-38059 Msa1411630:intron 45.0%
GAATCATCCCTAAACCTAGC+AGG + tig0023824:38050-38069 None:intergenic 45.0%
!! TCTCCAAAGTCTTTTGGGGA+TGG - tig0023824:38116-38135 Msa1411630:intron 45.0%
GAACCATCCCCAAAAGACTT+TGG + tig0023824:38122-38141 None:intergenic 45.0%
GGAAGACAAAGCTGTGTCAA+TGG - tig0023824:38195-38214 Msa1411630:intron 45.0%
TGTGTCAATGGCAGCATACA+GGG - tig0023824:38207-38226 Msa1411630:intron 45.0%
TTCTCCATGAGCAAAGTGAC+AGG + tig0023824:38252-38271 None:intergenic 45.0%
!! ATCTGGCTTGTTTCCACCAT+AGG - tig0023824:38692-38711 Msa1411630:intron 45.0%
AAACATGGCATTGCAGCCTA+TGG + tig0023824:38711-38730 None:intergenic 45.0%
ACAGCAGCTAGCCAAAAATC+TGG + tig0023824:38739-38758 None:intergenic 45.0%
! TGATAATGCTGCCCTATTGG+AGG - tig0023824:38776-38795 Msa1411630:intron 45.0%
! TTCCTGACAATGGGGTTGAT+GGG + tig0023824:39015-39034 None:intergenic 45.0%
ATTCCTGACAATGGGGTTGA+TGG + tig0023824:39016-39035 None:intergenic 45.0%
GAAACGCTAGATACTCCACT+TGG - tig0023824:39312-39331 Msa1411630:intron 45.0%
TTACCGACACCTATGTTGCA+TGG + tig0023824:39624-39643 None:intergenic 45.0%
! AAAAGCAGTCACTTCGGTGT+GGG + tig0023824:39649-39668 None:intergenic 45.0%
TAAAAGCAGTCACTTCGGTG+TGG + tig0023824:39650-39669 None:intergenic 45.0%
AGGTACATTCCTCATTTGCG+AGG - tig0023824:39832-39851 Msa1411630:CDS 45.0%
AAACTGTGGCCAAGACGTTA+AGG + tig0023824:39953-39972 None:intergenic 45.0%
! GTTGAGGCTTTTGGTGAAGT+TGG + tig0023824:40226-40245 None:intergenic 45.0%
ACTTCACCAAAAGCCTCAAC+AGG - tig0023824:40226-40245 Msa1411630:CDS 45.0%
ATCATTGGCAACACCTGTTG+AGG + tig0023824:40242-40261 None:intergenic 45.0%
AGTAGACTTGTGGTGAAGGT+AGG + tig0023824:40271-40290 None:intergenic 45.0%
CATGAGTAGACTTGTGGTGA+AGG + tig0023824:40275-40294 None:intergenic 45.0%
!!! GATTTTATTTTTTTAAATAA+AGG + tig0023824:39986-40005 None:intergenic 5.0%
TTGGATGAAGCAAACTGCGG+TGG + tig0023824:37579-37598 None:intergenic 50.0%
CTGAGCCACATTCCTTCAAG+AGG - tig0023824:37649-37668 Msa1411630:CDS 50.0%
AGCAATTCTCGCCCTCTTGA+AGG + tig0023824:37664-37683 None:intergenic 50.0%
GCTGATAACAATCCCCCTAG+TGG - tig0023824:37681-37700 Msa1411630:CDS 50.0%
GGGCATATCAATCCACTAGG+GGG + tig0023824:37696-37715 None:intergenic 50.0%
! GGAGGGCATATCAATCCACT+AGG + tig0023824:37699-37718 None:intergenic 50.0%
GGCTGAATCATCCTTGAAGG+AGG + tig0023824:37717-37736 None:intergenic 50.0%
GGAGGCTGAATCATCCTTGA+AGG + tig0023824:37720-37739 None:intergenic 50.0%
! ATGCTGCTTGTTCCTCGGTT+TGG + tig0023824:37744-37763 None:intergenic 50.0%
CCCGAATCTGCACTAAGTTC+AGG - tig0023824:37775-37794 Msa1411630:intron 50.0%
TTGATCCTCGCAACGACGAA+CGG - tig0023824:37886-37905 Msa1411630:intron 50.0%
AACGGCTTCAGATGGAGACA+AGG - tig0023824:37904-37923 Msa1411630:intron 50.0%
AAGGCAGCCACCACCAAATT+GGG - tig0023824:37923-37942 Msa1411630:intron 50.0%
TTCCAATAAGCGCTCCTTGC+TGG + tig0023824:38179-38198 None:intergenic 50.0%
CTGTGTCAATGGCAGCATAC+AGG - tig0023824:38206-38225 Msa1411630:intron 50.0%
ATACAGGGAGCTGTCCCTTT+GGG - tig0023824:38222-38241 Msa1411630:intron 50.0%
AAGTGACAGGTCTCCCCAAA+GGG + tig0023824:38239-38258 None:intergenic 50.0%
AAAGTGACAGGTCTCCCCAA+AGG + tig0023824:38240-38259 None:intergenic 50.0%
!! GAGACCTGTCACTTTGCTCA+TGG - tig0023824:38245-38264 Msa1411630:intron 50.0%
CACTTTGCTCATGGAGAAGC+AGG - tig0023824:38254-38273 Msa1411630:intron 50.0%
CAAAGGGGCAACAAACAGGA+GGG + tig0023824:38507-38526 None:intergenic 50.0%
AAGGCAAAGGGGCAACAAAC+AGG + tig0023824:38511-38530 None:intergenic 50.0%
TTCCGCGAAGACAAGGCAAA+GGG + tig0023824:38523-38542 None:intergenic 50.0%
TTTCCGCGAAGACAAGGCAA+AGG + tig0023824:38524-38543 None:intergenic 50.0%
! ATAGCGGGGCTACTGCATAT+AGG + tig0023824:38941-38960 None:intergenic 50.0%
TTCCCATCAACCCCATTGTC+AGG - tig0023824:39010-39029 Msa1411630:intron 50.0%
TCAGAGTTACAAGCTCCTGG+TGG - tig0023824:40145-40164 Msa1411630:CDS 50.0%
! GCAACACCTGTTGAGGCTTT+TGG + tig0023824:40235-40254 None:intergenic 50.0%
CATGACAAAGGAAGAGGAGC+AGG - tig0023824:40291-40310 Msa1411630:CDS 50.0%
GGTGGTGACATCCTACAGGT+TGG + tig0023824:37561-37580 None:intergenic 55.0%
CTCGCCCTCTTGAAGGAATG+TGG + tig0023824:37657-37676 None:intergenic 55.0%
ATTCAGCCTCCACCAAACCG+AGG - tig0023824:37729-37748 Msa1411630:CDS 55.0%
!! CTTGTTCCTCGGTTTGGTGG+AGG + tig0023824:37738-37757 None:intergenic 55.0%
!! CTGCTTGTTCCTCGGTTTGG+TGG + tig0023824:37741-37760 None:intergenic 55.0%
CTCACGGGGCTGATGAGATA+AGG - tig0023824:37835-37854 Msa1411630:intron 55.0%
ATGAGATAAGGCAGCCACCG+CGG - tig0023824:37847-37866 Msa1411630:intron 55.0%
ACTAGCTCCTGCCAATTCCG+CGG + tig0023824:37867-37886 None:intergenic 55.0%
CAACGACGAACGGCTTCAGA+TGG - tig0023824:37896-37915 Msa1411630:intron 55.0%
CAAGGCAGCCACCACCAAAT+TGG - tig0023824:37922-37941 Msa1411630:intron 55.0%
!! AGCCAGCAAGGAGCGCTTAT+TGG - tig0023824:38174-38193 Msa1411630:intron 55.0%
CATACAGGGAGCTGTCCCTT+TGG - tig0023824:38221-38240 Msa1411630:intron 55.0%
TACAGGGAGCTGTCCCTTTG+GGG - tig0023824:38223-38242 Msa1411630:intron 55.0%
GCAAAGGGGCAACAAACAGG+AGG + tig0023824:38508-38527 None:intergenic 55.0%
TCCGCGAAGACAAGGCAAAG+GGG + tig0023824:38522-38541 None:intergenic 55.0%
AGGCACTTTCCGCGAAGACA+AGG + tig0023824:38530-38549 None:intergenic 55.0%
CCATTGCAGCCTATGGTAGC+TGG + tig0023824:38665-38684 None:intergenic 55.0%
CCAGCTACCATAGGCTGCAA+TGG - tig0023824:38662-38681 Msa1411630:intron 55.0%
CATGGCATTGCAGCCTATGG+TGG + tig0023824:38708-38727 None:intergenic 55.0%
GAGTTACAAGCTCCTGGTGG+AGG - tig0023824:40148-40167 Msa1411630:CDS 55.0%
TCAGCTTCAATGCCTCCACC+AGG + tig0023824:40163-40182 None:intergenic 55.0%
CTGCGGTGGTGACATCCTAC+AGG + tig0023824:37565-37584 None:intergenic 60.0%
TAAGGCAGCCACCGCGGAAT+TGG - tig0023824:37853-37872 Msa1411630:intron 60.0%
TGAAGCCGTTCGTCGTTGCG+AGG + tig0023824:37894-37913 None:intergenic 60.0%
GGGCTGTGAACAAGCCAGCA+AGG - tig0023824:38162-38181 Msa1411630:intron 60.0%
GCCCCTTTGCCTTGTCTTCG+CGG - tig0023824:38518-38537 Msa1411630:intron 60.0%
AGCTCCTGCCAATTCCGCGG+TGG + tig0023824:37864-37883 None:intergenic 65.0%
GCAGCCACCGCGGAATTGGC+AGG - tig0023824:37857-37876 Msa1411630:intron 70.0%
Chromosome Type Strat End Strand Name
tig0023824 gene 37537 40390 37537 ID=Msa1411630;Name=Msa1411630
tig0023824 mRNA 37537 40390 37537 ID=Msa1411630-mRNA-1;Parent=Msa1411630;Name=Msa1411630-mRNA-1;_AED=0.04;_eAED=0.04;_QI=0|0|0|1|1|1|2|0|326
tig0023824 exon 39652 40390 39652 ID=Msa1411630-mRNA-1:exon:20227;Parent=Msa1411630-mRNA-1
tig0023824 exon 37537 37778 37537 ID=Msa1411630-mRNA-1:exon:20226;Parent=Msa1411630-mRNA-1
tig0023824 CDS 39652 40390 39652 ID=Msa1411630-mRNA-1:cds;Parent=Msa1411630-mRNA-1
tig0023824 CDS 37537 37778 37537 ID=Msa1411630-mRNA-1:cds;Parent=Msa1411630-mRNA-1
Gene Sequence

>Msa1411630

ATGAATTTTCCAGACGATATTCCAACCTGTAGGATGTCACCACCGCAGTTTGCTTCATCCAACAACGATATCATTGAAGTGAGGCCTCAAATCCCAATCAATAACGATCACTCTGAGCCACATTCCTTCAAGAGGGCGAGAATTGCTGATAACAATCCCCCTAGTGGATTGATATGCCCTCCTTCAAGGATGATTCAGCCTCCACCAAACCGAGGAACAAGCAGCATTTTTTTCAAGACCCGAATCTGCACTAAGTTCAGGTTTGGTACTTGTAGAAATGGCGAAAATTGCAATTACGCTCACGGGGCTGATGAGATAAGGCAGCCACCGCGGAATTGGCAGGAGCTAGTTGATCCTCGCAACGACGAACGGCTTCAGATGGAGACAAGGCAGCCACCACCAAATTGGGATGAAGATCAAAAAATCATTCACAAGATGAAGCTGTGCAAGAAGTATTACAACGGTGAGGAATGTCCTTATGGTGATAAGTGTAGCTTTCTTCATGAGGATCCTGCTAGGTTTAGGGATGATTCTTGGAAAACAAGAGAGTGTTCTGCAATAAGCATTGGAAATATTGGTTCTCCAAAGTCTTTTGGGGATGGTTCTAACAATTTGGAAGGCATTAGGGCTGTGAACAAGCCAGCAAGGAGCGCTTATTGGAAGACAAAGCTGTGTCAATGGCAGCATACAGGGAGCTGTCCCTTTGGGGAGACCTGTCACTTTGCTCATGGAGAAGCAGAGTTACAAGCTCCTGGTGGAGGCATTGAAGCTGAAGCTGCAGTTGCCATAGTAAGCTCAACAAAAGCTATCATTCCAACTTCACCAAAAGCCTCAACAGGTGTTGCCAATGATTCTACTCCTACCTTCACCACAAGTCTACTCATGACAAAGGAAGAGGAGCAGGATAAGAAGAAGCACTTACTTAGGTTGAAATTAGGCAAGAAGATCAATGGTATTTATGGTGATTGGATTGATGATTGA
Protein sequence

>Msa1411630

MNFPDDIPTCRMSPPQFASSNNDIIEVRPQIPINNDHSEPHSFKRARIADNNPPSGLICPPSRMIQPPPNRGTSSIFFKTRICTKFRFGTCRNGENCNYAHGADEIRQPPRNWQELVDPRNDERLQMETRQPPPNWDEDQKIIHKMKLCKKYYNGEECPYGDKCSFLHEDPARFRDDSWKTRECSAISIGNIGSPKSFGDGSNNLEGIRAVNKPARSAYWKTKLCQWQHTGSCPFGETCHFAHGEAELQAPGGGIEAEAAVAIVSSTKAIIPTSPKASTGVANDSTPTFTTSLLMTKEEEQDKKKHLLRLKLGKKINGIYGDWIDD*