AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1411640

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1411640 MtrunA17_Chr4g0063391 81.517 211 38 1 1 211 1 210 1.42e-126 358
Msa1411640 MtrunA17_Chr4g0063371 71.587 271 28 3 1 263 1 230 2.39e-124 353
Msa1411640 MtrunA17_Chr4g0063401 54.000 250 73 7 1 210 1 248 1.38e-78 238
Msa1411640 MtrunA17_Chr4g0063361 51.037 241 84 4 1 208 1 240 2.77e-76 232
Msa1411640 MtrunA17_Chr3g0098251 48.634 183 68 5 31 208 15 176 6.10e-47 155
Msa1411640 MtrunA17_Chr8g0360671 44.966 149 53 6 77 218 41 167 4.39e-30 110
Msa1411640 MtrunA17_Chr8g0366501 25.648 347 152 9 16 264 36 374 1.97e-26 105
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1411640 AT3G19360.1 38.926 298 113 10 31 264 81 373 3.20e-50 169
Msa1411640 AT3G19360.2 38.926 298 113 10 31 264 81 373 3.20e-50 169
Msa1411640 AT1G32360.1 26.351 296 130 8 54 264 90 382 8.90e-24 99.4

Find 72 sgRNAs with CRISPR-Local

Find 224 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AACCATTGAAACTAATAATT+TGG 0.147683 tig0023824:-53105 Msa1411640:CDS
CCAACTAGCTTCTGCGAATT+TGG 0.173447 tig0023824:+53320 None:intergenic
AGCCAGATCAATCGAATTTA+CGG 0.290260 tig0023824:-50728 Msa1411640:CDS
TCAATCGAATTTACGGTGAT+TGG 0.313071 tig0023824:-50721 Msa1411640:CDS
ATGAAGATCCTGCACAGTTT+AGG 0.341189 tig0023824:-53166 Msa1411640:CDS
CCAACTAAGAAGCGTATACT+TGG 0.350951 tig0023824:-50761 Msa1411640:CDS
TTCCAAATTATTAGTTTCAA+TGG 0.356757 tig0023824:+53103 None:intergenic
ATTTGTACTAAGTTCAGATT+TGG 0.359410 tig0023824:-53407 Msa1411640:CDS
GAAGCACTCTCTTGATTTCA+AGG 0.359778 tig0023824:+53133 None:intergenic
ATCGGCTGCAGCTGGAAAAT+CGG 0.361010 tig0023824:-53283 Msa1411640:CDS
CGACTAAACTGTGTCGCAAT+TGG 0.369653 tig0023824:-53037 Msa1411640:CDS
TGAACAACAATGACTTCAAA+AGG 0.386314 tig0023824:-53526 Msa1411640:CDS
TGCTATGGCAACTGCAGCTT+CGG 0.389652 tig0023824:+50799 None:intergenic
AAATCAGAGTTACAAGTTCC+TGG 0.400098 tig0023824:-50860 Msa1411640:intron
AGATTTGGTGCTTGTAGAAA+CGG 0.437088 tig0023824:-53392 Msa1411640:CDS
AACTGCAGCATCAGCTTCAA+TGG 0.437500 tig0023824:+50832 None:intergenic
AACTGCAGCTTCGGCTTCAA+TGG 0.440184 tig0023824:+50808 None:intergenic
TTAGAGGTTGGAATATCATA+TGG 0.441570 tig0023824:+53575 None:intergenic
TTGCGGCGGTGACATCTTAG+AGG 0.444480 tig0023824:+53559 None:intergenic
CTGGTGCTCTTCCTTTGCTA+TGG 0.445302 tig0023824:+50784 None:intergenic
CCAAGTATACGCTTCTTAGT+TGG 0.448187 tig0023824:+50761 None:intergenic
TAACGATGATCGGCTGCAGC+TGG 0.448905 tig0023824:-53291 Msa1411640:CDS
AGCAAGCAAGGGGCACTTAT+CGG 0.455209 tig0023824:-53061 Msa1411640:CDS
CACCGTAAATTCGATTGATC+TGG 0.457971 tig0023824:+50726 None:intergenic
TGTGCAAGCATTATTGCAAT+GGG 0.460308 tig0023824:-53226 Msa1411640:CDS
TCGGCTGCAGCTGGAAAATC+GGG 0.462737 tig0023824:-53282 Msa1411640:CDS
CTCTTTGCTCATGGTGAAGA+AGG 0.463092 tig0023824:-52978 Msa1411640:intron
CTGATCTGTTTGATTGAGGA+AGG 0.475163 tig0023824:+53453 None:intergenic
AATATACACTTATCTCCATA+TGG 0.477192 tig0023824:+53194 None:intergenic
GAGACAGGATATTGTTCCTA+TGG 0.480467 tig0023824:-53011 Msa1411640:CDS
CTCATGGAGCTGAAGAGATA+AGG 0.489689 tig0023824:-53352 Msa1411640:CDS
AATAATTGCAACTTTGCTCA+TGG 0.494959 tig0023824:-53368 Msa1411640:CDS
GATTGAGGAAGGGTCATCCT+TGG 0.496966 tig0023824:+53464 None:intergenic
CTTGGAGGAGGGCATATCAA+TGG 0.505795 tig0023824:+53482 None:intergenic
AGCTTCTGCGAATTTGGTGG+TGG 0.508361 tig0023824:+53326 None:intergenic
GGAATGTGAACAAGCAAGCA+AGG 0.508839 tig0023824:-53073 Msa1411640:CDS
CTAATAATTTGGAAGGCAGC+AGG 0.521839 tig0023824:-53094 Msa1411640:CDS
GTATACGCTTCTTAGTTGGC+TGG 0.524014 tig0023824:+50765 None:intergenic
AGGAGGGCATATCAATGGAT+TGG 0.527979 tig0023824:+53487 None:intergenic
CCAAATTCGCAGAAGCTAGT+TGG 0.528628 tig0023824:-53320 Msa1411640:CDS
GGAAGGGTCATCCTTGGAGG+AGG 0.531078 tig0023824:+53470 None:intergenic
TATTCATTCCTAAACTGTGC+AGG 0.531321 tig0023824:+53158 None:intergenic
CTGTGCAAGCATTATTGCAA+TGG 0.534714 tig0023824:-53227 Msa1411640:CDS
TGTCGCAATTGGAAGGAGAC+AGG 0.541370 tig0023824:-53026 Msa1411640:CDS
ACTAGCTTCTGCGAATTTGG+TGG 0.541605 tig0023824:+53323 None:intergenic
ATTGAAACTAATAATTTGGA+AGG 0.547647 tig0023824:-53101 Msa1411640:CDS
GAACAACAATGACTTCAAAA+GGG 0.550138 tig0023824:-53525 Msa1411640:CDS
AAAGAGACAGTTCTTTCCAT+AGG 0.554060 tig0023824:+52995 None:intergenic
TGAGGAAGGGTCATCCTTGG+AGG 0.556539 tig0023824:+53467 None:intergenic
CATTGATATGCCCTCCTCCA+AGG 0.556864 tig0023824:-53481 Msa1411640:CDS
GGCGGTGACATCTTAGAGGT+TGG 0.560713 tig0023824:+53563 None:intergenic
TTGGTCCGTGTAACGATGAT+CGG 0.560743 tig0023824:-53301 Msa1411640:CDS
AACAATGACTTCAAAAGGGA+AGG 0.561307 tig0023824:-53521 Msa1411640:CDS
TGGTCTGATCTGTTTGATTG+AGG 0.566957 tig0023824:+53449 None:intergenic
AAGAACTGTCTCTTTGCTCA+TGG 0.580294 tig0023824:-52987 Msa1411640:CDS
AATGGGGAGGAATGTCCATA+TGG 0.583975 tig0023824:-53209 Msa1411640:CDS
TCAGCTTCAATGGCTCCACC+AGG 0.589035 tig0023824:+50842 None:intergenic
TAAACTGTGTCGCAATTGGA+AGG 0.596328 tig0023824:-53033 Msa1411640:CDS
GAATGTGAACAAGCAAGCAA+GGG 0.597583 tig0023824:-53072 Msa1411640:CDS
AGCTGCAGTTGCCATAGCAA+AGG 0.598992 tig0023824:-50795 Msa1411640:CDS
ACTGTGCAGGATCTTCATGA+AGG 0.599205 tig0023824:+53171 None:intergenic
TGATCTGTTTGATTGAGGAA+GGG 0.618914 tig0023824:+53454 None:intergenic
GAAGGGTCATCCTTGGAGGA+GGG 0.622595 tig0023824:+53471 None:intergenic
AATATCATATGGAAAAGCCA+TGG 0.637338 tig0023824:+53586 None:intergenic
CAACTAAGAAGCGTATACTT+GGG 0.638379 tig0023824:-50760 Msa1411640:CDS
TTGTTCAATGAAGTGAATTG+CGG 0.641834 tig0023824:+53542 None:intergenic
AATGTGAACAAGCAAGCAAG+GGG 0.656040 tig0023824:-53071 Msa1411640:CDS
TTCAATGAAGTGAATTGCGG+CGG 0.657596 tig0023824:+53545 None:intergenic
CAAGCATTATTGCAATGGGG+AGG 0.662472 tig0023824:-53222 Msa1411640:CDS
TGCAGCCGATCATCGTTACA+CGG 0.666217 tig0023824:+53296 None:intergenic
TCAGAGTTACAAGTTCCTGG+TGG 0.718686 tig0023824:-50857 Msa1411640:intron
GTGCAAGCATTATTGCAATG+GGG 0.746686 tig0023824:-53225 Msa1411640:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAATTGAAATAAAATACTAA+AGG + tig0023824:51885-51904 None:intergenic 10.0%
!! AATATCAAAAAATCATACAA+AGG - tig0023824:51037-51056 Msa1411640:intron 15.0%
!! TATACTTTGATTATTTGTTT+CGG - tig0023824:51347-51366 Msa1411640:intron 15.0%
!!! TTTTTTATTTCTTTTAATCC+TGG - tig0023824:52234-52253 Msa1411640:intron 15.0%
!!! CAAGAGTTTTTTTAAATATA+AGG + tig0023824:53281-53300 None:intergenic 15.0%
!! AACCATTGAAACTAATAATT+TGG - tig0023824:51188-51207 Msa1411640:intron 20.0%
!!! TTCCAAATTATTAGTTTCAA+TGG + tig0023824:51193-51212 None:intergenic 20.0%
!! ATTGAAACTAATAATTTGGA+AGG - tig0023824:51192-51211 Msa1411640:intron 20.0%
!!! GTTTTGTACTAAATAAAGTT+TGG - tig0023824:52735-52754 Msa1411640:intron 20.0%
! ATTTGTACTAAGTTCAGATT+TGG - tig0023824:50886-50905 Msa1411640:intron 25.0%
! AATATACACTTATCTCCATA+TGG + tig0023824:51102-51121 None:intergenic 25.0%
! TTATCTTGCAAAAATGAGTT+GGG - tig0023824:51585-51604 Msa1411640:intron 25.0%
! AAAATACTAAAGGAAGAAGT+AGG + tig0023824:51875-51894 None:intergenic 25.0%
! CATGGCAATTAAAAATTCAA+TGG + tig0023824:52260-52279 None:intergenic 25.0%
! TGATCATTAATCATACATGA+AGG + tig0023824:52427-52446 None:intergenic 25.0%
! TATCAAATTTCTGCCATAAA+TGG - tig0023824:52461-52480 Msa1411640:intron 25.0%
! ATCAAATTTCTGCCATAAAT+GGG - tig0023824:52462-52481 Msa1411640:intron 25.0%
! AAAAATCTGGCATATAAACA+TGG + tig0023824:52567-52586 None:intergenic 25.0%
! AAGTTATCATAAGCAAAATG+CGG + tig0023824:52606-52625 None:intergenic 25.0%
! TTGTACTAAATAAAGTTTGG+TGG - tig0023824:52738-52757 Msa1411640:intron 25.0%
! TGTACTAAATAAAGTTTGGT+GGG - tig0023824:52739-52758 Msa1411640:intron 25.0%
! TATAAAAATTCCTGACAATG+CGG + tig0023824:52846-52865 None:intergenic 25.0%
! ATGATCCATTAGAATAAAGA+GGG + tig0023824:52911-52930 None:intergenic 25.0%
! AATGATCCATTAGAATAAAG+AGG + tig0023824:52912-52931 None:intergenic 25.0%
! AATGGATCATTACTATTGTA+TGG - tig0023824:52921-52940 Msa1411640:intron 25.0%
! TCTTAACAAATGTAAAACTG+TGG + tig0023824:52971-52990 None:intergenic 25.0%
!!! TACTAGAATTTGTTGTAGTT+TGG - tig0023824:52991-53010 Msa1411640:CDS 25.0%
!! ACTTTAAGAAGAAACAAAGT+TGG + tig0023824:53066-53085 None:intergenic 25.0%
!! TGACATGAACAATAATTTTG+AGG - tig0023824:53118-53137 Msa1411640:CDS 25.0%
TTAGAGGTTGGAATATCATA+TGG + tig0023824:50721-50740 None:intergenic 30.0%
TTGTTCAATGAAGTGAATTG+CGG + tig0023824:50754-50773 None:intergenic 30.0%
TGAACAACAATGACTTCAAA+AGG - tig0023824:50767-50786 Msa1411640:CDS 30.0%
GAACAACAATGACTTCAAAA+GGG - tig0023824:50768-50787 Msa1411640:CDS 30.0%
! TTCGTGTTTTGTAGAAAATG+TGG + tig0023824:50867-50886 None:intergenic 30.0%
AATAATTGCAACTTTGCTCA+TGG - tig0023824:50925-50944 Msa1411640:intron 30.0%
TGCAAATTACAGTGTTTGAA+TGG - tig0023824:51466-51485 Msa1411640:intron 30.0%
TGTGTATGATGTTTCATGTT+TGG - tig0023824:51521-51540 Msa1411640:intron 30.0%
! TTTTTGCAAGATAAGGCAAT+GGG + tig0023824:51579-51598 None:intergenic 30.0%
! ATTTTTGCAAGATAAGGCAA+TGG + tig0023824:51580-51599 None:intergenic 30.0%
!! CAACTCATTTTTGCAAGATA+AGG + tig0023824:51586-51605 None:intergenic 30.0%
CTTATCTTGCAAAAATGAGT+TGG - tig0023824:51584-51603 Msa1411640:intron 30.0%
AGTGGCGCATAAAAAATAAA+TGG - tig0023824:51665-51684 Msa1411640:intron 30.0%
CAATTAAAAATTCAATGGCC+AGG + tig0023824:52255-52274 None:intergenic 30.0%
TAAATGGACAGACATAGAAA+TGG - tig0023824:52307-52326 Msa1411640:intron 30.0%
! ATTTTCCATTCAGAACCTTA+GGG + tig0023824:52363-52382 None:intergenic 30.0%
! ATGATTCCATCCTTGTTTAT+AGG - tig0023824:52381-52400 Msa1411640:intron 30.0%
! ATGGCAGAAATTTGATAACA+AGG + tig0023824:52458-52477 None:intergenic 30.0%
! GTACTAAATAAAGTTTGGTG+GGG - tig0023824:52740-52759 Msa1411640:intron 30.0%
! ATGTAAGTGCTGTTAAATAG+CGG + tig0023824:52787-52806 None:intergenic 30.0%
TGATCCATTAGAATAAAGAG+GGG + tig0023824:52910-52929 None:intergenic 30.0%
GATGAACATAATACAATAGC+TGG + tig0023824:53039-53058 None:intergenic 30.0%
ATGTCAGACATCAAATTTGT+AGG + tig0023824:53104-53123 None:intergenic 30.0%
TTAAAACAACTGCAACTGTT+TGG - tig0023824:53365-53384 Msa1411640:CDS 30.0%
AACAATGACTTCAAAAGGGA+AGG - tig0023824:50772-50791 Msa1411640:CDS 35.0%
TGATCTGTTTGATTGAGGAA+GGG + tig0023824:50842-50861 None:intergenic 35.0%
!! AGATTTGGTGCTTGTAGAAA+CGG - tig0023824:50901-50920 Msa1411640:intron 35.0%
TGTGCAAGCATTATTGCAAT+GGG - tig0023824:51067-51086 Msa1411640:intron 35.0%
TATTCATTCCTAAACTGTGC+AGG + tig0023824:51138-51157 None:intergenic 35.0%
AAAGAGACAGTTCTTTCCAT+AGG + tig0023824:51301-51320 None:intergenic 35.0%
CATACATCTAAGCAAAGTAC+CGG + tig0023824:51642-51661 None:intergenic 35.0%
TTGCTTAGATGTATGACAAG+TGG - tig0023824:51647-51666 Msa1411640:intron 35.0%
GGCGCATAAAAAATAAATGG+TGG - tig0023824:51668-51687 Msa1411640:intron 35.0%
TGAGAAGAAGAACAGTGAAA+GGG + tig0023824:51714-51733 None:intergenic 35.0%
TTGAGAAGAAGAACAGTGAA+AGG + tig0023824:51715-51734 None:intergenic 35.0%
TTGTGGTTTCACAAATCTAC+CGG - tig0023824:52014-52033 Msa1411640:intron 35.0%
! CATTTTCCATTCAGAACCTT+AGG + tig0023824:52364-52383 None:intergenic 35.0%
ATAGTCTATACCATGATACC+CGG - tig0023824:52516-52535 Msa1411640:intron 35.0%
! AAAATGCGGATTAAGGTATC+TGG + tig0023824:52592-52611 None:intergenic 35.0%
! CATAAGCAAAATGCGGATTA+AGG + tig0023824:52599-52618 None:intergenic 35.0%
! ATGTTGTTAATTGCTGTTCG+CGG - tig0023824:52648-52667 Msa1411640:intron 35.0%
! TGTAAGTGCTGTTAAATAGC+GGG + tig0023824:52786-52805 None:intergenic 35.0%
TAACCCCCTCTTTATTCTAA+TGG - tig0023824:52903-52922 Msa1411640:intron 35.0%
GATCCATTAGAATAAAGAGG+GGG + tig0023824:52909-52928 None:intergenic 35.0%
CATATTTGACCCCAATATTG+AGG + tig0023824:53189-53208 None:intergenic 35.0%
CTCAATATTGGGGTCAAATA+TGG - tig0023824:53187-53206 Msa1411640:CDS 35.0%
AGGAGTCAGAACAATATACA+TGG + tig0023824:53394-53413 None:intergenic 35.0%
AAATCAGAGTTACAAGTTCC+TGG - tig0023824:53433-53452 Msa1411640:CDS 35.0%
! CAACTAAGAAGCGTATACTT+GGG - tig0023824:53533-53552 Msa1411640:CDS 35.0%
AGCCAGATCAATCGAATTTA+CGG - tig0023824:53565-53584 Msa1411640:CDS 35.0%
TCAATCGAATTTACGGTGAT+TGG - tig0023824:53572-53591 Msa1411640:CDS 35.0%
TTCAATGAAGTGAATTGCGG+CGG + tig0023824:50751-50770 None:intergenic 40.0%
CTGATCTGTTTGATTGAGGA+AGG + tig0023824:50843-50862 None:intergenic 40.0%
TGGTCTGATCTGTTTGATTG+AGG + tig0023824:50847-50866 None:intergenic 40.0%
CTGTGCAAGCATTATTGCAA+TGG - tig0023824:51066-51085 Msa1411640:intron 40.0%
GTGCAAGCATTATTGCAATG+GGG - tig0023824:51068-51087 Msa1411640:intron 40.0%
ATGAAGATCCTGCACAGTTT+AGG - tig0023824:51127-51146 Msa1411640:intron 40.0%
! GAAGCACTCTCTTGATTTCA+AGG + tig0023824:51163-51182 None:intergenic 40.0%
CTAATAATTTGGAAGGCAGC+AGG - tig0023824:51199-51218 Msa1411640:intron 40.0%
GAATGTGAACAAGCAAGCAA+GGG - tig0023824:51221-51240 Msa1411640:intron 40.0%
AATGTGAACAAGCAAGCAAG+GGG - tig0023824:51222-51241 Msa1411640:intron 40.0%
TAAACTGTGTCGCAATTGGA+AGG - tig0023824:51260-51279 Msa1411640:intron 40.0%
GAGACAGGATATTGTTCCTA+TGG - tig0023824:51282-51301 Msa1411640:intron 40.0%
AAGAACTGTCTCTTTGCTCA+TGG - tig0023824:51306-51325 Msa1411640:intron 40.0%
AAACTGGAGGAAATCACTAC+TGG + tig0023824:51618-51637 None:intergenic 40.0%
! AGTGATTTCCTCCAGTTTTC+CGG - tig0023824:51620-51639 Msa1411640:intron 40.0%
!! TAAGCAAAGTACCGGAAAAC+TGG + tig0023824:51634-51653 None:intergenic 40.0%
GTTGTATGTTGTTAGCGTTG+TGG - tig0023824:51997-52016 Msa1411640:intron 40.0%
AGATGAGTCTCCTTTGTTCA+CGG - tig0023824:52190-52209 Msa1411640:intron 40.0%
CTTTACAGATGCATGACACA+TGG + tig0023824:52278-52297 None:intergenic 40.0%
CATGCATCTGTAAAGAGAAC+CGG - tig0023824:52283-52302 Msa1411640:intron 40.0%
ATGCATCTGTAAAGAGAACC+GGG - tig0023824:52284-52303 Msa1411640:intron 40.0%
TGTAAAGAGAACCGGGTAAA+TGG - tig0023824:52291-52310 Msa1411640:intron 40.0%
TCTATGTCTGTCCATTTACC+CGG + tig0023824:52305-52324 None:intergenic 40.0%
ACATAGAAATGGAGCAGATC+TGG - tig0023824:52318-52337 Msa1411640:intron 40.0%
TGCAAAAATGTACCTCCCTA+AGG - tig0023824:52345-52364 Msa1411640:intron 40.0%
GCTATGCCTATAAACAAGGA+TGG + tig0023824:52390-52409 None:intergenic 40.0%
! TCCTTGTTTATAGGCATAGC+AGG - tig0023824:52390-52409 Msa1411640:intron 40.0%
ACCTGCTATGCCTATAAACA+AGG + tig0023824:52394-52413 None:intergenic 40.0%
! AAGGTATCTGGCGAAAAATC+TGG + tig0023824:52580-52599 None:intergenic 40.0%
TCTATAGCACTATTGCAGAG+CGG + tig0023824:52696-52715 None:intergenic 40.0%
ATGCCGTGATCCTCAATATT+GGG - tig0023824:53176-53195 Msa1411640:CDS 40.0%
TGTTCTGACTCCTGAAGTAT+CGG - tig0023824:53401-53420 Msa1411640:CDS 40.0%
CCAAGTATACGCTTCTTAGT+TGG + tig0023824:53535-53554 None:intergenic 40.0%
! CCAACTAAGAAGCGTATACT+TGG - tig0023824:53532-53551 Msa1411640:CDS 40.0%
CACCGTAAATTCGATTGATC+TGG + tig0023824:53570-53589 None:intergenic 40.0%
! AGGAGGGCATATCAATGGAT+TGG + tig0023824:50809-50828 None:intergenic 45.0%
CTCATGGAGCTGAAGAGATA+AGG - tig0023824:50941-50960 Msa1411640:intron 45.0%
! ACTAGCTTCTGCGAATTTGG+TGG + tig0023824:50973-50992 None:intergenic 45.0%
! CCAACTAGCTTCTGCGAATT+TGG + tig0023824:50976-50995 None:intergenic 45.0%
CCAAATTCGCAGAAGCTAGT+TGG - tig0023824:50973-50992 Msa1411640:intron 45.0%
TTGGTCCGTGTAACGATGAT+CGG - tig0023824:50992-51011 Msa1411640:intron 45.0%
CAAGCATTATTGCAATGGGG+AGG - tig0023824:51071-51090 Msa1411640:intron 45.0%
AATGGGGAGGAATGTCCATA+TGG - tig0023824:51084-51103 Msa1411640:intron 45.0%
ACTGTGCAGGATCTTCATGA+AGG + tig0023824:51125-51144 None:intergenic 45.0%
GGAATGTGAACAAGCAAGCA+AGG - tig0023824:51220-51239 Msa1411640:intron 45.0%
CGACTAAACTGTGTCGCAAT+TGG - tig0023824:51256-51275 Msa1411640:intron 45.0%
CTCTTTGCTCATGGTGAAGA+AGG - tig0023824:51315-51334 Msa1411640:intron 45.0%
AAGGCAATGGGACAACAAGT+AGG + tig0023824:51567-51586 None:intergenic 45.0%
GACACACCATTACAGAAGGA+GGG + tig0023824:51760-51779 None:intergenic 45.0%
TGACACACCATTACAGAAGG+AGG + tig0023824:51761-51780 None:intergenic 45.0%
!!! AAGAGTGACGGGTTTTCACA+GGG + tig0023824:51787-51806 None:intergenic 45.0%
AATATCCCTGTCACACAGCA+TGG - tig0023824:52110-52129 Msa1411640:intron 45.0%
ATGGAAGCTGTGACATGTGA+AGG - tig0023824:52129-52148 Msa1411640:intron 45.0%
TGTGACATGTGAAGGCCAAA+CGG - tig0023824:52137-52156 Msa1411640:intron 45.0%
AGTCTCCTTTGTTCACGGAA+TGG - tig0023824:52195-52214 Msa1411640:intron 45.0%
GTCTCCTTTGTTCACGGAAT+GGG - tig0023824:52196-52215 Msa1411640:intron 45.0%
TCTCCTTTGTTCACGGAATG+GGG - tig0023824:52197-52216 Msa1411640:intron 45.0%
AAACCCCATTCCGTGAACAA+AGG + tig0023824:52203-52222 None:intergenic 45.0%
!! TACCTCCCTAAGGTTCTGAA+TGG - tig0023824:52355-52374 Msa1411640:intron 45.0%
TTCCATTCAGAACCTTAGGG+AGG + tig0023824:52360-52379 None:intergenic 45.0%
!! TTTCTGCCATAAATGGGCTG+TGG - tig0023824:52468-52487 Msa1411640:intron 45.0%
GGCATATAAACATGGAGCTG+CGG + tig0023824:52559-52578 None:intergenic 45.0%
! TTGCTGTTCGCGGAAAAATG+TGG - tig0023824:52658-52677 Msa1411640:intron 45.0%
TTCCTGACAATGCGGTTGAT+GGG + tig0023824:52838-52857 None:intergenic 45.0%
ATTCCTGACAATGCGGTTGA+TGG + tig0023824:52839-52858 None:intergenic 45.0%
CAATATTGAGGATCACGGCA+TGG + tig0023824:53177-53196 None:intergenic 45.0%
CATGCCGTGATCCTCAATAT+TGG - tig0023824:53175-53194 Msa1411640:CDS 45.0%
TGCCGTGATCCTCAATATTG+GGG - tig0023824:53177-53196 Msa1411640:CDS 45.0%
GACCCCAATATTGAGGATCA+CGG + tig0023824:53182-53201 None:intergenic 45.0%
TGCAAGACAACCGATACTTC+AGG + tig0023824:53414-53433 None:intergenic 45.0%
TCAGAGTTACAAGTTCCTGG+TGG - tig0023824:53436-53455 Msa1411640:CDS 45.0%
AACTGCAGCATCAGCTTCAA+TGG + tig0023824:53464-53483 None:intergenic 45.0%
GTATACGCTTCTTAGTTGGC+TGG + tig0023824:53531-53550 None:intergenic 45.0%
! CTTGGAGGAGGGCATATCAA+TGG + tig0023824:50814-50833 None:intergenic 50.0%
CATTGATATGCCCTCCTCCA+AGG - tig0023824:50812-50831 Msa1411640:CDS 50.0%
GATTGAGGAAGGGTCATCCT+TGG + tig0023824:50832-50851 None:intergenic 50.0%
! AGCTTCTGCGAATTTGGTGG+TGG + tig0023824:50970-50989 None:intergenic 50.0%
TGCAGCCGATCATCGTTACA+CGG + tig0023824:51000-51019 None:intergenic 50.0%
ATCGGCTGCAGCTGGAAAAT+CGG - tig0023824:51010-51029 Msa1411640:intron 50.0%
!! AGCAAGCAAGGGGCACTTAT+CGG - tig0023824:51232-51251 Msa1411640:intron 50.0%
TGTCGCAATTGGAAGGAGAC+AGG - tig0023824:51267-51286 Msa1411640:intron 50.0%
GCAATGGGACAACAAGTAGG+AGG + tig0023824:51564-51583 None:intergenic 50.0%
!! GCAAAGTACCGGAAAACTGG+AGG + tig0023824:51631-51650 None:intergenic 50.0%
CCATTACAGAAGGAGGGAGA+AGG + tig0023824:51754-51773 None:intergenic 50.0%
CCTTCTCCCTCCTTCTGTAA+TGG - tig0023824:51751-51770 Msa1411640:intron 50.0%
CGGTGACACACCATTACAGA+AGG + tig0023824:51764-51783 None:intergenic 50.0%
!!! CAAGAGTGACGGGTTTTCAC+AGG + tig0023824:51788-51807 None:intergenic 50.0%
! TGGTGACTTGCAAGAGTGAC+GGG + tig0023824:51798-51817 None:intergenic 50.0%
! GTGGTGACTTGCAAGAGTGA+CGG + tig0023824:51799-51818 None:intergenic 50.0%
CCATTGGGGGATTTAAGTGG+TGG + tig0023824:51818-51837 None:intergenic 50.0%
CCACCACTTAAATCCCCCAA+TGG - tig0023824:51815-51834 Msa1411640:intron 50.0%
! GTGCCATTGGGGGATTTAAG+TGG + tig0023824:51821-51840 None:intergenic 50.0%
! ACTCGTAACGACGTGCCATT+GGG + tig0023824:51833-51852 None:intergenic 50.0%
AACTCGTAACGACGTGCCAT+TGG + tig0023824:51834-51853 None:intergenic 50.0%
! CCTACGACGGAACACTTTTC+CGG + tig0023824:52061-52080 None:intergenic 50.0%
!! GAAAAGTGTTCCGTCGTAGG+TGG - tig0023824:52061-52080 Msa1411640:intron 50.0%
AGCTTCCATGCTGTGTGACA+GGG + tig0023824:52118-52137 None:intergenic 50.0%
TTCACGGAATGGGGTTTCAC+AGG - tig0023824:52206-52225 Msa1411640:intron 50.0%
!! ATAAATGGGCTGTGGCATGG+CGG - tig0023824:52476-52495 Msa1411640:intron 50.0%
!! ATGGCGGACTTTGTGCTAGT+CGG - tig0023824:52492-52511 Msa1411640:intron 50.0%
TGGAAACAAGCCGGGTATCA+TGG + tig0023824:52529-52548 None:intergenic 50.0%
! GCATATGGTGGAAACAAGCC+GGG + tig0023824:52537-52556 None:intergenic 50.0%
! GGCATATGGTGGAAACAAGC+CGG + tig0023824:52538-52557 None:intergenic 50.0%
AAACATGGAGCTGCGGCATA+TGG + tig0023824:52552-52571 None:intergenic 50.0%
TTCCCATCAACCGCATTGTC+AGG - tig0023824:52833-52852 Msa1411640:intron 50.0%
AACTGCAGCTTCGGCTTCAA+TGG + tig0023824:53488-53507 None:intergenic 50.0%
TGCTATGGCAACTGCAGCTT+CGG + tig0023824:53497-53516 None:intergenic 50.0%
AGCTGCAGTTGCCATAGCAA+AGG - tig0023824:53498-53517 Msa1411640:CDS 50.0%
CTGGTGCTCTTCCTTTGCTA+TGG + tig0023824:53512-53531 None:intergenic 50.0%
GGCGGTGACATCTTAGAGGT+TGG + tig0023824:50733-50752 None:intergenic 55.0%
TTGCGGCGGTGACATCTTAG+AGG + tig0023824:50737-50756 None:intergenic 55.0%
GAAGGGTCATCCTTGGAGGA+GGG + tig0023824:50825-50844 None:intergenic 55.0%
TGAGGAAGGGTCATCCTTGG+AGG + tig0023824:50829-50848 None:intergenic 55.0%
TAACGATGATCGGCTGCAGC+TGG - tig0023824:51002-51021 Msa1411640:intron 55.0%
TCGGCTGCAGCTGGAAAATC+GGG - tig0023824:51011-51030 Msa1411640:intron 55.0%
CAGAAGGAGGGAGAAGGAGA+AGG + tig0023824:51748-51767 None:intergenic 55.0%
!!! AGTGACGGGTTTTCACAGGG+CGG + tig0023824:51784-51803 None:intergenic 55.0%
! TCGTAACGACGTGCCATTGG+GGG + tig0023824:51831-51850 None:intergenic 55.0%
! CTCGTAACGACGTGCCATTG+GGG + tig0023824:51832-51851 None:intergenic 55.0%
!! CCGGAAAAGTGTTCCGTCGT+AGG - tig0023824:52058-52077 Msa1411640:intron 55.0%
AAGTGTTCCGTCGTAGGTGG+CGG - tig0023824:52064-52083 Msa1411640:intron 55.0%
CAGCTTCCATGCTGTGTGAC+AGG + tig0023824:52119-52138 None:intergenic 55.0%
GACATGTGAAGGCCAAACGG+CGG - tig0023824:52140-52159 Msa1411640:intron 55.0%
!! GCCATAAATGGGCTGTGGCA+TGG - tig0023824:52473-52492 Msa1411640:intron 55.0%
GCCATGCCACAGCCCATTTA+TGG + tig0023824:52477-52496 None:intergenic 55.0%
TCTCAGCCACACCACAGTCA+TGG - tig0023824:53248-53267 Msa1411640:CDS 55.0%
AAGGTCTGCGACCATGACTG+TGG + tig0023824:53262-53281 None:intergenic 55.0%
TCAGCTTCAATGGCTCCACC+AGG + tig0023824:53454-53473 None:intergenic 55.0%
GGAAGGGTCATCCTTGGAGG+AGG + tig0023824:50826-50845 None:intergenic 60.0%
!! CAAATCTACCGGCTCCGCTG+CGG - tig0023824:52025-52044 Msa1411640:intron 60.0%
AGTGTTCCGTCGTAGGTGGC+GGG - tig0023824:52065-52084 Msa1411640:intron 60.0%
CATGGAGCTGCGGCATATGG+TGG + tig0023824:52549-52568 None:intergenic 60.0%
CTGCGACCATGACTGTGGTG+TGG + tig0023824:53257-53276 None:intergenic 60.0%
CCGGATTCCGATCACCGCAG+CGG + tig0023824:52042-52061 None:intergenic 65.0%
CCGCTGCGGTGATCGGAATC+CGG - tig0023824:52039-52058 Msa1411640:intron 65.0%
GTGTTCCGTCGTAGGTGGCG+GGG - tig0023824:52066-52085 Msa1411640:intron 65.0%
CCTTTCCCCGCCACCTACGA+CGG + tig0023824:52074-52093 None:intergenic 65.0%
AAGGCCAAACGGCGGCGATG+CGG - tig0023824:52148-52167 Msa1411640:intron 65.0%
GCGGCGATGCGGTGGCTAAA+CGG - tig0023824:52159-52178 Msa1411640:intron 65.0%
! ACCGGCTCCGCTGCGGTGAT+CGG - tig0023824:52032-52051 Msa1411640:intron 70.0%
TCCGATCACCGCAGCGGAGC+CGG + tig0023824:52036-52055 None:intergenic 70.0%
!! CCGTCGTAGGTGGCGGGGAA+AGG - tig0023824:52071-52090 Msa1411640:intron 70.0%
GCCAAACGGCGGCGATGCGG+TGG - tig0023824:52151-52170 Msa1411640:intron 75.0%
GCCACCGCATCGCCGCCGTT+TGG + tig0023824:52155-52174 None:intergenic 75.0%
Chromosome Type Strat End Strand Name
tig0023824 gene 50709 53606 50709 ID=Msa1411640;Name=Msa1411640
tig0023824 mRNA 50709 53606 50709 ID=Msa1411640-mRNA-1;Parent=Msa1411640;Name=Msa1411640-mRNA-1;_AED=0.25;_eAED=0.25;_QI=0|0|0|1|1|1|2|0|264
tig0023824 exon 52979 53606 52979 ID=Msa1411640-mRNA-1:exon:20235;Parent=Msa1411640-mRNA-1
tig0023824 exon 50709 50875 50709 ID=Msa1411640-mRNA-1:exon:20234;Parent=Msa1411640-mRNA-1
tig0023824 CDS 52979 53606 52979 ID=Msa1411640-mRNA-1:cds;Parent=Msa1411640-mRNA-1
tig0023824 CDS 50709 50875 50709 ID=Msa1411640-mRNA-1:cds;Parent=Msa1411640-mRNA-1
Gene Sequence

>Msa1411640

ATGGCTTTTCCATATGATATTCCAACCTCTAAGATGTCACCGCCGCAATTCACTTCATTGAACAACAATGACTTCAAAAGGGAAGGAACTTCTCATTCCAATCCATTGATATGCCCTCCTCCAAGGATGACCCTTCCTCAATCAAACAGATCAGACCACATTTTCTACAAAACACGAATTTGTACTAAGTTCAGATTTGGTGCTTGTAGAAACGGAAATAATTGCAACTTTGCTCATGGAGCTGAAGAGATAAGGCAACCACCACCAAATTCGCAGAAGCTAGTTGGTCCGTGTAACGATGATCGGCTGCAGCTGGAAAATCGGGCTGAATATCAAAAAATCATACAAAGGATGAAACTGTGCAAGCATTATTGCAATGGGGAGGAATGTCCATATGGAGATAAGTGTATATTCCTTCATGAAGATCCTGCACAGTTTAGGAATGAATATTCCTTGAAATCAAGAGAGTGCTTCGCAATAACCATTGAAACTAATAATTTGGAAGGCAGCAGGAATGTGAACAAGCAAGCAAGGGGCACTTATCGGACGACTAAACTGTGTCGCAATTGGAAGGAGACAGGATATTGTTCCTATGGAAAGAACTGTCTCTTTGCTCATGGTGAAGAAGAGTTACAAGTTCCTGGTGGAGCCATTGAAGCTGATGCTGCAGTTGCCATTGAAGCCGAAGCTGCAGTTGCCATAGCAAAGGAAGAGCACCAGCCAACTAAGAAGCGTATACTTGGGCTGAAATTCAGCCAGATCAATCGAATTTACGGTGATTGGATTGATGATTGA
Protein sequence

>Msa1411640

MAFPYDIPTSKMSPPQFTSLNNNDFKREGTSHSNPLICPPPRMTLPQSNRSDHIFYKTRICTKFRFGACRNGNNCNFAHGAEEIRQPPPNSQKLVGPCNDDRLQLENRAEYQKIIQRMKLCKHYCNGEECPYGDKCIFLHEDPAQFRNEYSLKSRECFAITIETNNLEGSRNVNKQARGTYRTTKLCRNWKETGYCSYGKNCLFAHGEEELQVPGGAIEADAAVAIEAEAAVAIAKEEHQPTKKRILGLKFSQINRIYGDWIDD*