AlfalfaGEDB | Msa1416020

M. sativa cultivar zhongmu-4 / Msa1416020

KEGG: K13464
GO: GO:0000003 GO:0001101 GO:0003006 GO:0003674 GO:0003712 GO:0003714 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006950 GO:0006952 GO:0007154 GO:0007165 GO:0007275 GO:0008150 GO:0009555 GO:0009605 GO:0009607 GO:0009611 GO:0009617 GO:0009719 GO:0009725 GO:0009753 GO:0009755 GO:0009791 GO:0009867 GO:0009889 GO:0009890 GO:0009892 GO:0009908 GO:0009966 GO:0009987 GO:0010033 GO:0010556 GO:0010558 GO:0010605 GO:0010646 GO:0019219 GO:0019222 GO:0022414 GO:0023051 GO:0023052 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0031347 GO:0032501 GO:0032502 GO:0032870 GO:0042221 GO:0042742 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464 GO:0045934 GO:0048229 GO:0048367 GO:0048519 GO:0048523 GO:0048583 GO:0048608 GO:0048731 GO:0048856 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051172 GO:0051252 GO:0051253 GO:0051704 GO:0051707 GO:0051716 GO:0060255 GO:0061458 GO:0065007 GO:0070887 GO:0071229 GO:0071310 GO:0071395 GO:0071495 GO:0080090 GO:0080134 GO:0090567 GO:0098542 GO:0140110 GO:1900067 GO:1901700 GO:1901701 GO:1902679 GO:1903506 GO:1903507 GO:2000022 GO:2001141
PFAM: PF06200.17

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1416020	sp\|Q8H395\|TI10B_ORYSJ	36.810	163	65	6	1	129	71	229	7.01e-20	84.7

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1416020	G7JS77	74.419	129	22	2	1	121	1	126	9.24e-55	177

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TFs/TRs:

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Gene ID	Type	Classification
Msa1416020	TF	Tify

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1416020	MtrunA17_Chr4g0070271	70.000	130	38	1	1	129	67	196	3.58e-55	171
Msa1416020	MtrunA17_Chr4g0070241	90.625	64	6	0	1	64	1	64	3.26e-36	119
Msa1416020	MtrunA17_Chr2g0301131	37.569	181	54	6	1	129	72	245	3.50e-27	101
Msa1416020	MtrunA17_Chr5g0399851	36.434	129	58	3	25	129	11	139	8.02e-20	79.3
Msa1416020	MtrunA17_Chr5g0399881	41.176	119	51	4	30	129	39	157	1.47e-18	77.0
Msa1416020	MtrunA17_Chr5g0399861	37.288	118	56	2	30	129	39	156	8.94e-18	74.7

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1416020	AT1G19180.2	40.000	120	45	3	30	122	53	172	8.06e-17	73.2
Msa1416020	AT1G19180.1	40.000	120	45	3	30	122	119	238	1.28e-16	73.6
Msa1416020	AT1G19180.3	40.000	120	45	3	30	122	119	238	1.28e-16	73.6

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Find 23 sgRNAs with CRISPR-Local

Find 142 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAGTATGGAGTGGATTCAGT+AGG	0.584677	tig0024312:-14093	Msa1416010:intergenic
AATGTCAACTGTGCAGTTTC+CGG	0.323565	tig0024312:-12946	Msa1416020:intergenic
AGCTCCTCAACTTTGTCAGC+AGG	0.455494	tig0024312:-13006	Msa1416020:intergenic
AGCTTCACTTCTTCGATTCA+TGG	0.237608	tig0024312:+13239	Msa1416020:CDS
CAACCACCTCCTACACTAAC+TGG	0.374106	tig0024312:+13084	Msa1416020:CDS
CAATTACCAGTTAGTGTAGG+AGG	0.522487	tig0024312:-13090	Msa1416020:intergenic
CACCTAAAACAAGTATGGAG+TGG	0.594681	tig0024312:-14103	Msa1416010:intergenic
CATTTGCAAGCACTCAAACT+CGG	0.560353	tig0024312:+13034	Msa1416020:CDS
CTCATATCAGGAATAACTGA+AGG	0.598557	tig0024312:-13057	Msa1416010:intergenic
CTGTGGATTCTTCGTCTCCA+AGG	0.562813	tig0024312:+12771	Msa1416020:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
TAACTCAATGGTTAACAAAC+CGG	+	tig0024312:12927-12946	Msa1416020:CDS	30.0%
GTTGTCGTAACTTTGTAATT+TGG	+	tig0024312:13678-13697	Msa1416020:intron	30.0%
TAGAATTGGATAGAATCAGT+TGG	-	tig0024312:13810-13829	Msa1416020:intergenic	30.0%
TAACTCAATGGTTAACAAAC+CGG	+	tig0024312:12927-12946	Msa1416020:CDS	30.0%
GTTGTCGTAACTTTGTAATT+TGG	+	tig0024312:13678-13697	Msa1416020:intron	30.0%
TAGAATTGGATAGAATCAGT+TGG	-	tig0024312:13810-13829	Msa1416020:intergenic	30.0%
CTCATATCAGGAATAACTGA+AGG	-	tig0024312:13060-13079	Msa1416020:intergenic	35.0%
TTCTTCGATTCATGGAGAAA+AGG	+	tig0024312:13247-13266	Msa1416020:CDS	35.0%
ACTGCAAAGTAAGTAACAAG+AGG	-	tig0024312:13945-13964	Msa1416020:intergenic	35.0%
ATTTCCTGAGTGATGTTGTT+TGG	+	tig0024312:13965-13984	Msa1416020:intron	35.0%

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Chromosome	Type	Strat	End	Strand	Name
tig0024312	gene	12728	14122	12728	ID=Msa1416020;Name=Msa1416020
tig0024312	mRNA	12728	14122	12728	ID=Msa1416020-mRNA-1;Parent=Msa1416020;Name=Msa1416020-mRNA-1;_AED=0.28;_eAED=0.29;_QI=0\|0\|0\|0.75\|1\|1\|4\|0\|130
tig0024312	exon	12728	12792	12728	ID=Msa1416020-mRNA-1:exon:22729;Parent=Msa1416020-mRNA-1
tig0024312	exon	12921	13105	12921	ID=Msa1416020-mRNA-1:exon:22730;Parent=Msa1416020-mRNA-1
tig0024312	exon	13220	13277	13220	ID=Msa1416020-mRNA-1:exon:22731;Parent=Msa1416020-mRNA-1
tig0024312	exon	14038	14122	14038	ID=Msa1416020-mRNA-1:exon:22732;Parent=Msa1416020-mRNA-1
tig0024312	CDS	12728	12792	12728	ID=Msa1416020-mRNA-1:cds;Parent=Msa1416020-mRNA-1
tig0024312	CDS	12921	13105	12921	ID=Msa1416020-mRNA-1:cds;Parent=Msa1416020-mRNA-1
tig0024312	CDS	13220	13277	13220	ID=Msa1416020-mRNA-1:cds;Parent=Msa1416020-mRNA-1
tig0024312	CDS	14038	14122	14038	ID=Msa1416020-mRNA-1:cds;Parent=Msa1416020-mRNA-1

Gene Sequence

>Msa1416020

ATGGACTTATTTCCCACAAACGTGACACCAAAAAACGTTACACCTGTGGATTCTTCGTCTCCAAGAACTAATAACTCAATGGTTAACAAACCGGAAACTGCACAGTTGACATTGTTTTATAATGGAGAAGTTATTGTATTGGATGATTTTCCTGCTGACAAAGTTGAGGAGCTTAAGTCATTTGCAAGCACTCAAACTCGGCCTTCAGTTATTCCTGATATGAGTCTTCAACCACCTCCTACACTAACTGATATGGTAATTGCTAGAAAAGCTTCACTTCTTCGATTCATGGAGAAAAGGAAAGCCAGAGTTTCTGCCAAATCACCATATCAAAGAAGCGATTCCGACTCTGCTCCAAAAAAACCTACTGAATCCACTCCATACTTGTTTTAG

Protein sequence

>Msa1416020

MDLFPTNVTPKNVTPVDSSSPRTNNSMVNKPETAQLTLFYNGEVIVLDDFPADKVEELKSFASTQTRPSVIPDMSLQPPPTLTDMVIARKASLLRFMEKRKARVSAKSPYQRSDSDSAPKKPTESTPYLF*