AlfalfaGEDB | Msa1416400

M. sativa cultivar zhongmu-4 / Msa1416400

KEGG: K08065|K14799
GO: GO:0000976 GO:0000981 GO:0001067 GO:0001654 GO:0001932 GO:0001934 GO:0003674 GO:0003676 GO:0003677 GO:0003690 GO:0003700 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005667 GO:0006139 GO:0006351 GO:0006355 GO:0006357 GO:0006366 GO:0006725 GO:0006807 GO:0007275 GO:0007399 GO:0007423 GO:0008134 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009889 GO:0009891 GO:0009893 GO:0009966 GO:0009967 GO:0009987 GO:0010033 GO:0010467 GO:0010468 GO:0010556 GO:0010557 GO:0010562 GO:0010604 GO:0010628 GO:0010646 GO:0010647 GO:0010720 GO:0010941 GO:0016070 GO:0016602 GO:0018130 GO:0019216 GO:0019218 GO:0019219 GO:0019220 GO:0019222 GO:0019438 GO:0022008 GO:0022603 GO:0023051 GO:0023056 GO:0030154 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031399 GO:0031401 GO:0031974 GO:0031981 GO:0032268 GO:0032270 GO:0032501 GO:0032502 GO:0032774 GO:0032991 GO:0032993 GO:0034097 GO:0034641 GO:0034645 GO:0034654 GO:0042221 GO:0042325 GO:0042327 GO:0042981 GO:0043066 GO:0043067 GO:0043069 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043408 GO:0043410 GO:0043565 GO:0044212 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0044798 GO:0044877 GO:0045540 GO:0045595 GO:0045597 GO:0045664 GO:0045666 GO:0045676 GO:0045678 GO:0045893 GO:0045935 GO:0045937 GO:0046483 GO:0046532 GO:0046534 GO:0046890 GO:0048513 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048583 GO:0048584 GO:0048699 GO:0048731 GO:0048749 GO:0048856 GO:0048869 GO:0050767 GO:0050769 GO:0050789 GO:0050793 GO:0050794 GO:0050810 GO:0050896 GO:0051094 GO:0051171 GO:0051173 GO:0051174 GO:0051239 GO:0051240 GO:0051246 GO:0051247 GO:0051252 GO:0051254 GO:0051716 GO:0051960 GO:0051962 GO:0060255 GO:0060284 GO:0060548 GO:0062012 GO:0065007 GO:0070013 GO:0070491 GO:0070887 GO:0071310 GO:0071345 GO:0071704 GO:0080090 GO:0090181 GO:0090304 GO:0090575 GO:0097159 GO:0097659 GO:0106118 GO:0140110 GO:1901360 GO:1901362 GO:1901363 GO:1901576 GO:1902531 GO:1902533 GO:1902680 GO:1902930 GO:1903506 GO:1903508 GO:1990823 GO:1990830 GO:1990837 GO:2000026 GO:2000027 GO:2000112 GO:2001141
PFAM: PF00808.26

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1416400	sp\|O82248\|NFYB5_ARATH	72.277	101	24	2	8	105	46	145	1.85e-48	155

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1416400	G7JKC3	89.815	108	7	2	14	118	23	129	2.08e-63	198

TFs/TRs:

Gene ID	Type	Classification
Msa1416400	TF	NF-YB

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1416400	MtrunA17_Chr2g0296321	81.308	107	14	3	14	117	67	170	2.59e-55	172
Msa1416400	MtrunA17_Chr4g0067091	90.217	92	5	2	14	102	23	113	1.65e-54	169
Msa1416400	MtrunA17_Chr1g0195851	77.895	95	17	2	14	105	37	130	2.10e-50	158
Msa1416400	MtrunA17_Chr2g0290491	60.185	108	39	2	14	118	18	124	1.13e-41	135
Msa1416400	MtrunA17_Chr4g0076321	66.316	95	28	2	14	105	20	113	1.50e-41	134
Msa1416400	MtrunA17_Chr1g0191981	68.085	94	26	2	15	105	6	98	3.45e-41	132
Msa1416400	MtrunA17_Chr3g0102351	63.542	96	31	2	14	106	28	122	3.05e-40	132
Msa1416400	MtrunA17_Chr1g0185911	67.778	90	25	2	14	100	30	118	7.54e-40	130
Msa1416400	MtrunA17_Chr5g0446491	61.386	101	35	2	9	106	19	118	1.00e-39	130
Msa1416400	MtrunA17_Chr7g0263601	64.130	92	29	2	14	102	27	117	3.15e-39	129
Msa1416400	MtrunA17_Chr8g0382931	56.604	106	42	2	8	110	23	127	4.49e-39	129
Msa1416400	MtrunA17_Chr8g0384451	63.158	95	31	2	14	105	41	134	7.44e-39	129
Msa1416400	MtrunA17_Chr4g0076381	56.075	107	43	2	14	117	59	164	3.07e-37	126
Msa1416400	MtrunA17_Chr1g0165041	59.574	94	34	2	14	104	7	99	9.14e-34	115
Msa1416400	MtrunA17_Chr5g0446601	43.182	88	46	2	16	100	27	113	3.65e-18	75.9
Msa1416400	MtrunA17_Chr1g0159271	41.379	87	47	2	27	110	1	86	8.10e-16	67.8
Msa1416400	MtrunA17_Chr1g0158951	45.070	71	38	1	34	104	13	82	4.37e-15	65.9
Msa1416400	MtrunA17_Chr2g0307551	30.612	98	64	2	5	99	3	99	1.01e-13	63.5
Msa1416400	MtrunA17_Chr1g0159291	35.802	81	48	2	18	95	7	86	1.16e-12	58.9
Msa1416400	MtrunA17_Chr4g0062271	28.571	98	66	2	5	99	3	99	2.90e-12	59.7

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1416400	AT2G47810.1	72.277	101	24	2	8	105	46	145	1.88e-49	155
Msa1416400	AT1G09030.1	69.792	96	25	2	14	106	4	98	7.78e-44	140
Msa1416400	AT5G47640.1	66.337	101	30	2	9	106	23	122	7.65e-42	136
Msa1416400	AT4G14540.1	62.745	102	34	2	11	109	19	119	1.20e-41	135
Msa1416400	AT3G53340.3	60.952	105	37	2	8	109	24	127	1.67e-41	134
Msa1416400	AT3G53340.2	60.952	105	37	2	8	109	24	127	1.67e-41	134
Msa1416400	AT3G53340.5	63.265	98	32	2	8	102	24	120	5.49e-40	131
Msa1416400	AT3G53340.1	63.265	98	32	2	8	102	24	120	5.49e-40	131
Msa1416400	AT2G38880.3	67.391	92	26	2	14	102	22	112	5.74e-40	130
Msa1416400	AT2G38880.2	67.391	92	26	2	14	102	22	112	7.78e-40	130
Msa1416400	AT2G38880.1	67.391	92	26	2	14	102	22	112	7.78e-40	130
Msa1416400	AT2G38880.5	67.391	92	26	2	14	102	22	112	7.78e-40	130
Msa1416400	AT3G53340.4	62.887	97	32	2	8	101	24	119	2.21e-39	128
Msa1416400	AT2G37060.2	62.245	98	33	2	8	102	25	121	5.48e-39	129
Msa1416400	AT2G37060.3	62.245	98	33	2	8	102	25	121	5.48e-39	129
Msa1416400	AT2G37060.1	62.245	98	33	2	8	102	25	121	5.48e-39	129
Msa1416400	AT2G38880.4	65.934	91	27	2	14	101	22	111	1.00e-38	126
Msa1416400	AT2G38880.7	66.667	90	26	2	14	100	22	110	1.80e-38	126
Msa1416400	AT2G13570.1	64.211	95	30	2	14	105	37	130	2.65e-38	128
Msa1416400	AT2G38880.11	65.934	91	27	2	14	101	22	111	2.91e-38	127
Msa1416400	AT2G38880.8	65.934	91	27	2	14	101	22	111	2.91e-38	127
Msa1416400	AT2G38880.9	65.934	91	27	2	14	101	22	111	3.46e-38	126
Msa1416400	AT2G38880.10	65.934	91	27	2	14	101	22	111	3.46e-38	126
Msa1416400	AT2G38880.6	65.934	91	27	2	14	101	22	111	3.46e-38	126
Msa1416400	AT5G47670.2	61.053	95	33	2	14	105	30	123	2.65e-36	123
Msa1416400	AT5G47670.1	61.053	95	33	2	14	105	59	152	4.05e-36	123
Msa1416400	AT5G47670.3	61.053	95	33	2	14	105	59	152	4.05e-36	123
Msa1416400	AT1G21970.1	53.684	95	40	2	14	105	60	153	4.24e-31	110
Msa1416400	AT5G23090.4	28.037	107	71	3	5	106	3	108	6.34e-12	59.3
Msa1416400	AT5G23090.5	28.155	103	68	3	5	102	3	104	7.94e-11	56.2
Msa1416400	AT5G23090.2	28.155	103	68	3	5	102	3	104	7.94e-11	56.2
Msa1416400	AT5G23090.1	28.155	103	68	3	5	102	3	104	7.94e-11	56.2

Find 22 sgRNAs with CRISPR-Local

Find 25 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CACTCTTGCATTGTTTCCTT+TGG	0.194156	tig0024351:+17043	None:intergenic
GCATAGATATAGAGAATTAG+AGG	0.332962	tig0024351:-16870	Msa1416400:CDS
ACTCTTGCATTGTTTCCTTT+GGG	0.333372	tig0024351:+17044	None:intergenic
CGATGATATATGTTGGGCTT+TGG	0.361762	tig0024351:-16939	Msa1416400:CDS
GATGATATATGTTGGGCTTT+GGG	0.371039	tig0024351:-16938	Msa1416400:CDS
TGAATATTATTAGTAGTAGT+TGG	0.374870	tig0024351:+16836	None:intergenic
ATCCTCCCAACATTTGCAAT+TGG	0.423560	tig0024351:+17100	None:intergenic
TGTTGGGCTTTGGGGACACT+TGG	0.433132	tig0024351:-16929	Msa1416400:CDS
TTGTTACCAATTGCAAATGT+TGG	0.438960	tig0024351:-17106	Msa1416400:CDS
TGTTACCAATTGCAAATGTT+GGG	0.447901	tig0024351:-17105	Msa1416400:CDS
TCCTTTAATAACGTTGAAAA+CGG	0.453836	tig0024351:-17143	Msa1416400:CDS
ACTAATAATATTCAAAATAG+AGG	0.455896	tig0024351:-16827	Msa1416400:CDS
GAAAGGAGAAAGACAGTGAA+TGG	0.488041	tig0024351:-16965	Msa1416400:CDS
TGAAAACGGAGGCACAAGAT+AGG	0.505295	tig0024351:-17129	Msa1416400:CDS
CAGAGAAATGCAGGAAGGAA+AGG	0.518431	tig0024351:-16982	Msa1416400:CDS
ATGATATATGTTGGGCTTTG+GGG	0.541154	tig0024351:-16937	Msa1416400:CDS
GTGACGCATCAGAGAAATGC+AGG	0.541912	tig0024351:-16991	Msa1416400:CDS
TGGTGACGATGATATATGTT+GGG	0.557816	tig0024351:-16945	Msa1416400:CDS
TTTAATAACGTTGAAAACGG+AGG	0.598517	tig0024351:-17140	Msa1416400:CDS
TACCAATTGCAAATGTTGGG+AGG	0.600846	tig0024351:-17102	Msa1416400:CDS
CGCATCAGAGAAATGCAGGA+AGG	0.623973	tig0024351:-16987	Msa1416400:CDS
ATGGTGACGATGATATATGT+TGG	0.624511	tig0024351:-16946	Msa1416400:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	ACTAATAATATTCAAAATAG+AGG	-	tig0024351:17147-17166	Msa1416400:CDS	15.0%
!!	TGAATATTATTAGTAGTAGT+TGG	+	tig0024351:17141-17160	None:intergenic	20.0%
!	TCCTTTAATAACGTTGAAAA+CGG	-	tig0024351:16831-16850	Msa1416400:CDS	25.0%
!	TCCGTTTTCAACGTTATTAA+AGG	+	tig0024351:16835-16854	None:intergenic	30.0%
	TTTAATAACGTTGAAAACGG+AGG	-	tig0024351:16834-16853	Msa1416400:CDS	30.0%
	TTGTTACCAATTGCAAATGT+TGG	-	tig0024351:16868-16887	Msa1416400:CDS	30.0%
	TGTTACCAATTGCAAATGTT+GGG	-	tig0024351:16869-16888	Msa1416400:CDS	30.0%
	GCATAGATATAGAGAATTAG+AGG	-	tig0024351:17104-17123	Msa1416400:CDS	30.0%
!!	TTTGGGATTTTTGCATTCTG+TGG	+	tig0024351:16916-16935	None:intergenic	35.0%
	ACAGAATGCAAAAATCCCAA+AGG	-	tig0024351:16915-16934	Msa1416400:CDS	35.0%
	ACTCTTGCATTGTTTCCTTT+GGG	+	tig0024351:16933-16952	None:intergenic	35.0%
!	ATGGTGACGATGATATATGT+TGG	-	tig0024351:17028-17047	Msa1416400:CDS	35.0%
!	TGGTGACGATGATATATGTT+GGG	-	tig0024351:17029-17048	Msa1416400:CDS	35.0%
	GATGATATATGTTGGGCTTT+GGG	-	tig0024351:17036-17055	Msa1416400:CDS	35.0%
	ATGATATATGTTGGGCTTTG+GGG	-	tig0024351:17037-17056	Msa1416400:CDS	35.0%
	TACCAATTGCAAATGTTGGG+AGG	-	tig0024351:16872-16891	Msa1416400:CDS	40.0%
	ATCCTCCCAACATTTGCAAT+TGG	+	tig0024351:16877-16896	None:intergenic	40.0%
	CACTCTTGCATTGTTTCCTT+TGG	+	tig0024351:16934-16953	None:intergenic	40.0%
	GAAAGGAGAAAGACAGTGAA+TGG	-	tig0024351:17009-17028	Msa1416400:CDS	40.0%
	CGATGATATATGTTGGGCTT+TGG	-	tig0024351:17035-17054	Msa1416400:CDS	40.0%
!	TGAAAACGGAGGCACAAGAT+AGG	-	tig0024351:16845-16864	Msa1416400:CDS	45.0%
	CAGAGAAATGCAGGAAGGAA+AGG	-	tig0024351:16992-17011	Msa1416400:CDS	45.0%
	GTGACGCATCAGAGAAATGC+AGG	-	tig0024351:16983-17002	Msa1416400:CDS	50.0%
	CGCATCAGAGAAATGCAGGA+AGG	-	tig0024351:16987-17006	Msa1416400:CDS	50.0%
!!	TGTTGGGCTTTGGGGACACT+TGG	-	tig0024351:17045-17064	Msa1416400:CDS	55.0%

Chromosome	Type	Strat	End	Strand	Name
tig0024351	gene	16820	17176	16820	ID=Msa1416400;Name=Msa1416400
tig0024351	mRNA	16820	17176	16820	ID=Msa1416400-mRNA-1;Parent=Msa1416400;Name=Msa1416400-mRNA-1;_AED=0.02;_eAED=0.08;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|118
tig0024351	exon	16820	17176	16820	ID=Msa1416400-mRNA-1:exon:22954;Parent=Msa1416400-mRNA-1
tig0024351	CDS	16820	17176	16820	ID=Msa1416400-mRNA-1:cds;Parent=Msa1416400-mRNA-1

Gene Sequence	>Msa1416400 TTGGAGGAAGTTCCTTTAATAACGTTGAAAACGGAGGCACAAGATAGGTTGTTACCAATTGCAAATGTTGGGAGGATAATGAAGCAGATATTACCACAGAATGCAAAAATCCCAAAGGAAACAATGCAAGAGTGTGTGTCAGAGTTTATAAGCTTTGTGACAAGTGACGCATCAGAGAAATGCAGGAAGGAAAGGAGAAAGACAGTGAATGGTGACGATGATATATGTTGGGCTTTGGGGACACTTGGTTTTGATGATTATGCTGAACAAATAAGAAAGTATTTGCATAGATATAGAGAATTAGAGGTAGAAAGAACACCAACTACTACTAATAATATTCAAAATAGAGGAAATTAA
Protein sequence	>Msa1416400 LEEVPLITLKTEAQDRLLPIANVGRIMKQILPQNAKIPKETMQECVSEFISFVTSDASEKCRKERRKTVNGDDDICWALGTLGFDDYAEQIRKYLHRYRELEVERTPTTTNNIQNRGN*